Unveiling Bone and Dental Regeneration Potential of Quince Seed Mucilage-Nanohydroxyapatite Scaffolds in Rabbit Mandibles

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Cigdem Cetin Genc, Hilal Deniz Yılmaz-Dagdeviren, Yesim Deniz, Burak Derkus, Alpin Degirmenci, Yavuz Emre Arslan
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Abstract

Donor-side morbidity of autografting for maxillofacial region defect regeneration has directed attention to bioengineered scaffolds. Composite scaffolds that mimic the bone extracellular matrix (ECM) are the potential candidates for defect reconstruction. Herein, a plant-based regenerative hydrogel, quince seed mucilage (QSM), was enriched with the nanohydroxyapatite (nHAp) particles to construct composite scaffolds (QSM/nHAp). The emerging scaffold is able to induce cellular spheroid formation and regenerate the critical-sized bilateral mandibular defects in rabbits. The macroscopic observations, histochemical (HC) and immunohistochemical (IHC) stainings, μ-computer tomography (CT) scanning, quantitative real time-polymerase chain reaction (qRT-PCR) analyses, and scanning electron microscopy (SEM) imaging revealed that all QSM/nHAp scaffolds were swelled with host blood, filled the whole cavity, and sustained cellular infiltration without adverse reactions. The gradual biodegradation profile of the scaffolds improved bone regeneration by releasing nHAp particles from the scaffold. Strikingly, co-development of dental and bone regeneration was observed for all QSM/nHAp groups beginning after day 21. Moreover, QSM/nHAp scaffolds induced expression (> 2-fold) of bone and dental-related gene and protein expressions at the grafted area and sustained a proper platform for maxillofacial remodeling. Therefore, we strongly believe that such biocompatible plant-based constructs, compared with conventional medical devices used in maxillofacial surgery, could support and induce simultaneous bone and dental regeneration due to the intrinsic dynamics of the material.

Abstract Image

揭示木瓜籽黏液-纳米羟基磷灰石支架在兔下颌骨的骨和牙再生潜力
自体骨移植修复颌面部缺损引起了人们对生物工程支架的关注。模拟骨细胞外基质(ECM)的复合支架是修复骨缺损的潜在候选材料。本研究采用纳米羟基磷灰石(nHAp)颗粒富集的植物再生水凝胶——木瓜籽胶(QSM)构建复合支架(QSM/nHAp)。新出现的支架能够诱导细胞球体形成并再生兔临界大小的双侧下颌骨缺损。宏观观察、组织化学(HC)和免疫组织化学(IHC)染色、μ-计算机断层扫描(CT)、定量实时聚合酶链反应(qRT-PCR)分析和扫描电镜(SEM)成像显示,所有QSM/nHAp支架均被宿主血液充盈,充满整个腔体,细胞持续浸润,无不良反应。支架的逐渐生物降解特性通过从支架中释放nHAp颗粒来改善骨再生。引人注目的是,从第21天开始,所有QSM/nHAp组都观察到牙齿和骨再生的共同发展。此外,QSM/nHAp支架在移植区域诱导了2倍的骨和牙相关基因和蛋白的表达,为颌面部重塑提供了合适的平台。因此,我们坚信,与用于颌面外科的传统医疗器械相比,由于材料的内在动力学,这种生物相容性的植物基结构可以支持和诱导骨和牙的同时再生。
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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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