In Vitro Degradation and Cytocompatibility Study of Biodegradable Porous Zinc Scaffolds Fabricated by Fused Deposition Modeling Based Rapid Tooling Method

IF 3.2 4区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2025-05-12 DOI:10.1002/jbm.b.35593

Abhishek Kansal, Sangita Mahapatra, Akshay Dvivedi, Pradeep Kumar, Mayank Goswami

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Abstract

An integrative study is conducted on the static biodegradation behavior and cytocompatibility of organized porous network structured (OPNS) zinc scaffolds fabricated via the Fused Deposition Modeling based Rapid Tooling (FDM-RT) method. The degradation study investigates the corrosion mechanism over immersion time in simulated body fluid (SBF), whereas the cytocompatibility study incorporates MTT assay and direct cell counting tests. The results indicate that the scaffold morphology, including scaffold struts, interconnectivity, or porosity, along with the immersion period, significantly influence the degradation behavior. The static corrosion rates are determined to be 0.27 ± 0.006, 0.79 ± 0.01, and 1.35 ± 0.05 mm y⁻¹ for bulk and porous zinc samples after 28 days. The higher corrosion rate of porous zinc alloys is mainly due to their higher surface area, which enhances exposure to the solution. Over time, protective layers form on both porous and bulk samples, leading to a gradual decrease in corrosion rate. The XRD results reveal that the corrosion protective layer is primarily composed of ZnO, Ca₃(PO₄)₂, Zn (OH)₂, and Zn₃(PO₄)₂·4H₂O. Moreover, MTT assay and direct cell counting of mouse fibroblast 3T3 cells on bulk and porous zinc samples suggest that the prepared zinc samples have potential for tissue engineering applications.

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基于熔融沉积建模的快速模具法制备可生物降解多孔锌支架的体外降解及细胞相容性研究

采用基于熔融沉积建模的快速加工（FDM-RT）方法，对组织多孔网络结构（OPNS）锌支架的静态生物降解行为和细胞相容性进行了综合研究。降解研究研究了在模拟体液（SBF）中浸泡时间的腐蚀机制，而细胞相容性研究包括MTT试验和直接细胞计数试验。结果表明，支架的形态，包括支架支撑、互连性或孔隙度，随着浸泡时间的延长，对降解行为有显著影响。28天后，体积锌和多孔锌的静态腐蚀速率分别为0.27±0.006、0.79±0.01和1.35±0.05 mm y - 1。多孔锌合金较高的腐蚀速率主要是由于其较大的表面积，增加了与溶液的接触。随着时间的推移，多孔和散装样品上形成保护层，导致腐蚀速率逐渐降低。XRD结果表明，腐蚀保护层主要由ZnO、Ca3(PO4)2、Zn (OH)2和Zn3(PO4)2·4H2O组成。此外，MTT实验和小鼠成纤维细胞3T3细胞在散装和多孔锌样品上的直接细胞计数表明，制备的锌样品具有组织工程应用的潜力。

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来源期刊

Journal of biomedical materials research. Part B, Applied biomaterials 工程技术-材料科学：生物材料

CiteScore

7.50

自引率

2.90%

发文量

199

审稿时长

12 months

期刊介绍： Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.