Journal of biomedical materials research. Part B, Applied biomaterials最新文献

{"title":"Comparison of Outcomes After Anterior Cervical Discectomy and Fusion Using Bioactive Glass–Ceramic Spacer-7 (NOVOMAX-FUSION) and an Allograft Spacer: A Retrospective, Case-Matched, Multicenter Study","authors":"Gi-Wuk Jang,&nbsp;Kyung-Hyun Kim,&nbsp;Dong-Ah Shin,&nbsp;Hyun-Joon Jang,&nbsp;Chang-Kyu Lee,&nbsp;Dong-Kyu Chin,&nbsp;Jeong-Yoon Park,&nbsp;Seong-Wook Koo,&nbsp;Bong-Ju Moon","doi":"10.1002/jbm.b.35521","DOIUrl":"10.1002/jbm.b.35521","url":null,"abstract":"<div>\u0000 \u0000 <p>The bioactive glass–ceramic spacer (BGS)-7, a biosynthetic intervertebral fusion material introduced in 2014, has not been the subject of comparative clinical studies on anterior cervical discectomy and fusion (ACDF) surgery. This study, for the first time, aims to compare the radiological and clinical outcomes of the renewed BGS-7, released in 2019, with those of an allograft spacer. The comparison includes a finite element analysis of the biomechanical properties of each implant, adding a novel dimension to the research. We prospectively followed up on 29 patients who underwent ACDF using BGS-7 as the experimental group. To select a control group for comparison, 253 patients with level 1 ACDF with an allograft spacer between 2012 and 2022 were selected from our hospital. Using propensity score matching, 27 and 54 patients in the BGS-7 and allograft groups, respectively, were selected. The average subsidence length was 1.02 ± 1.44 mm per level in the BGS-7 group and 2.27 ± 2.25 mm per level in the allograft group. Subsidence was observed in 14 of 54 patients (25.9%) in the allograft group and one of 27 patients (3.7%) in the BGS-7 group (<i>p</i> = 0.016). In the allograft group, 16 of the 54 patients (29.6%) monitored for 6 months achieved satisfactory fusion outcomes with grades 4 and 5. Thirty-eight of 54 patients (70.4%) followed up for &gt; 1 year in the allograft group achieved adequate fusion outcomes with grades 4 and 5. In the BGS-7 group, 17 of the 27 patients (63.0%) monitored for 6 months achieved satisfactory fusion results with grades 4 and 5. Twenty-three of the 27 patients (85.2%) followed up for &gt; 1 year obtained adequate fusion outcomes with grades 4 and 5. There was a significant difference in the fusion rates between the two groups at 6 months (<i>p</i> = 0.008). BGS-7 is a reliable instrument for ACDF with no instances of instrumental failure. The BGS-7 group had positive clinical outcomes after surgery without any untoward events, and an early fusion rate with the creation of a bone bridge was noted during the 6-month follow-up period. Our findings not only indicate the safety of BGS-7 but also its practicality as a substitute for allografts in ACDF, instilling confidence in its application.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Workshop on the Latest Advances in Biomedical Applications of Octacalcium Phosphate","authors":"Nicola Döbelin,&nbsp;Osamu Suzuki,&nbsp;Christophe Drouet,&nbsp;Jānis Ločs,&nbsp;Gerard Insley,&nbsp;Philip Procter","doi":"10.1002/jbm.b.35500","DOIUrl":"10.1002/jbm.b.35500","url":null,"abstract":"<p>The first workshop on the “latest advances in biomedical applications of octacalcium phosphate (OCP)” was organized as a satellite symposium to the Bioceramics33 conference in Solothurn, Switzerland, in October 2023. The event brought together leading researchers and industry representatives to present and discuss their latest groundbreaking research aimed at developing and commercializing advanced OCP-based biomaterials for bone regeneration. The topics presented by the six invited speakers ranged from a fundamental understanding of the OCP crystal chemistry to advanced processing and characterization methods, functionalization, biomineralization, and commercialization. With this summary report, we are laying the foundation for a continuation of a series of workshops on the subject of OCP biomaterials in order to promote the exchange between researchers and industry representatives and to drive forward the development and commercialization of new improved synthetic bone substitute materials.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35500","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finite Element and Density Functional Theory Modeling Effectively Predict Pitting Degradation of Hydroxyapatite-Coated Pure Magnesium","authors":"Reese A. Dunne,&nbsp;Doyl E. Dickel,&nbsp;Addison M. Green,&nbsp;Dam Kim,&nbsp;Lauren B. Priddy,&nbsp;Matthew W. Priddy","doi":"10.1002/jbm.b.35519","DOIUrl":"10.1002/jbm.b.35519","url":null,"abstract":"<div>\u0000 \u0000 <p>The emergence of degradable orthopedic implants for fracture fixation may abrogate the need for implant removal surgery and minimize pain associated with permanent implants. Magnesium (Mg) and its alloys are being explored as a biomaterial for degradable implants due to mechanical properties similar to those of bone. Previous in vitro studies have determined the degradation rate of pure Mg to be relatively fast when compared to bone regeneration. Hydroxyapatite (HA), the mineral component of bone, may serve as a surface coating on Mg-based implants to effectively slow and control the degradation rate. The objective of this work was to develop and implement a finite element (FE) model that utilizes a damage evolution law for pitting corrosion to predict the degradation of pure Mg (non-coated) and HA-coated pure Mg (coated) materials simulated in physiological conditions. Finite element analysis (FEA) was performed on a cylindrical Mg specimen (25.4 mm diameter, 8 mm height) through Abaqus/Standard software to incrementally monitor the damage value of each Mg element and subsequently delete fully-degraded elements from the simulation. A Fortran user-material (UMAT) subroutine assigned each element a pitting parameter, controlling the rate of degradation throughout the simulation and providing necessary inputs of elastic material properties and degradation model parameters for pure Mg and HA into Abaqus. The simulations allowed for the visualization of both pure Mg and HA-coated pure Mg degradation over a 120-day period, displaying expected degradation trends such as lower corrosion rates for HA-coated Mg and degradation propagating from the edges inward. Simulation results were calibrated with our prior results from a 30-day experimental degradation study via direct comparison with mass loss over time. Additionally, lower length scale, density functional theory (DFT) simulations were performed to provide physical meaning for the model pitting parameter. The FE simulation was extended to model resin-enclosed pure Mg and HA-coated pure Mg degradation, where only the top surface of the specimen was exposed to the corrosion surface, for investigating changes in Mg surface roughness (height) over time. The impacts of this work include the establishment of a computational model of pure Mg and HA-coated pure Mg degradation calibrated using in vitro degradation data to advance the use of Mg-based biomaterials, and more broadly, to predict degradation rates of next-generation orthopedic implants.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Systematic Enhancement of Microbial Decontamination Efficiency in Bone Graft Processing by Means of High Hydrostatic Pressure Using Escherichia coli as a Model Organism","authors":"","doi":"10.1002/jbm.b.35505","DOIUrl":"10.1002/jbm.b.35505","url":null,"abstract":"<p>H. Loeffler, J. Waletzko-Hellwig, R. Fischer, et al., “Systematic Enhancement of Microbial Decontamination Efficiency in Bone Graft Processing by Means of High Hydrostatic Pressure Using Escherichia coli as a Model Organism,” Journal of Biomedical Materials Research Part B 112 (2024): e35383. https://doi.org/10.1002/jbm.b.35383.</p><p>A recalibration of the high hydrostatic pressure device after the publication of this paper revealed incorrect pressure levels stated in this article. As this is a systematic error, it is necessary to correct the pressure levels by the factor of 0.7. Accordingly, 250 MPa now corresponds to 175 MPa, 350 MPa was corrected to 245 MPa and 600 MPa is now 420 MPa, suggesting that the efficiency of the treatment was higher than initially assumed.</p><p>A detailed listing of the affected text passages can be found below. Figures as well as Figure captions were corrected and attached, accordingly.</p><p>We apologize for this error.\u0000 </p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of Empty Lacunae in Tissue Sections of Osteonecrosis of the Femoral Head Using YOLOv8 Artificial Intelligence Model","authors":"Issei Shinohara,&nbsp;Atsuyuki Inui,&nbsp;Masatoshi Murayama,&nbsp;Yosuke Susuki,&nbsp;Qi Gao,&nbsp;Simon Kwoon-Ho Chow,&nbsp;Yutaka Mifune,&nbsp;Tomoyuki Matsumoto,&nbsp;Ryosuke Kuroda,&nbsp;Stuart B. Goodman","doi":"10.1002/jbm.b.35512","DOIUrl":"https://doi.org/10.1002/jbm.b.35512","url":null,"abstract":"<div>\u0000 \u0000 <p>Histomorphometry is an important technique in the evaluation of non-traumatic osteonecrosis of the femoral head (ONFH). Quantification of empty lacunae and pyknotic cells on histological images is the most reliable measure of ONFH pathology, yet it is time and manpower consuming. This study focused on the application of artificial intelligence (AI) technology to tissue image evaluation. The aim of this study is to establish an automated cell counting platform using YOLOv8 as an object detection model on ONFH tissue images and to evaluate and validate its accuracy. From 30 ONFH model rabbits, 270 tissue images were prepared; based on evaluations by three researchers, ground truth labels were created to classify each cell in the image into two classes (osteocytes and empty lacunae) or three classes (osteocytes, pyknotic cells, and empty lacunae). Two and three classes were then annotated on each image. Transfer learning based on annotated data (80% for training and 20% for validation) was performed using YOLOv8n and YOLOv8x with different parameters. To evaluate the detection accuracy of the training model, the mean average precision (mAP (50)) and precision-recall curve were identified. In addition, the reliability of cell counting by YOLOv8 relative to manual cell counting was evaluated by linear regression analysis using five histological images unused in previous experiments. The mAP (50) for the detection of empty lacunae was 0.868 for the YOLOv8n and 0.883 for the YOLOv8x. The mAP (50) for the three classes was 0.735 for the YOLOv8n model and 0.750 for the YOLOv8x model. The quantification of empty lacunae by automated cell counting obtained in the learning was highly correlated with the manual counting data. The development of an AI-applied automated cell counting platform will significantly reduce the time and effort of manual cell counting in histological analysis.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titanium Implant Modified With Zinc-Doped Carbon Dot Layer as an Innovative Coating for the Development of Local Drug Delivery System for Ciprofloxacin","authors":"Jakub Reczkowski,&nbsp;Łukasz Ławniczak,&nbsp;Maria Ratajczak,&nbsp;Adam Voelkel,&nbsp;Mariusz Sandomierski","doi":"10.1002/jbm.b.35517","DOIUrl":"10.1002/jbm.b.35517","url":null,"abstract":"<div>\u0000 \u0000 <p>This study presents a new innovative drug delivery system for ciprofloxacin, which is based on the formation of a zinc-doped carbon dots layer on the surface of a titanium alloy (TiAl4V6). In the study, the effectiveness of the synthesis method of a zinc-doped carbon dots layer was determined. The distribution of the layer of carbon dots on the surface of the titanium alloy was investigated using the FT-IR mapping technique, which confirmed the efficiency of the synthesis. The effective synthesis of carbon dots and the coordination of zinc ions on their surface opens the possibility of sorption of ciprofloxacin, which results in a high application potential of the obtained biomaterial. The introduction of zinc cations on the surface of the carbon dots layer resulted in high sorption results of the active substance (40 μg of drug per 1 cm² of implant). The release profile of ciprofloxacin from the modified surface of the titanium alloy indicates that this active substance can be released for up to 4 h. The biomaterial obtained in this work is also hydrophilic (about 40°), which was shown by the contact angle tests. This is an important feature and indicates a high application potential of the performed modification. The resulting layer has antibacterial properties. Growth inhibition for microorganisms such as <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Bacillus cereus</i>, and <i>Candida albicans</i> ranged from 74% to 96%. The creation of such a layer on the titanium alloy may reduce the risk of infection during the implantation procedure.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation and Fatigue Behavior of 3D-Printed Bioresorbable Tracheal Splints","authors":"Jenna M. Wahbeh,&nbsp;John Lama,&nbsp;Sang-Hyun Park,&nbsp;Edward Ebramzadeh,&nbsp;Scott J. Hollister,&nbsp;Sophia N. Sangiorgio","doi":"10.1002/jbm.b.35501","DOIUrl":"10.1002/jbm.b.35501","url":null,"abstract":"<p>Severe infantile tracheobronchomalacia (TBM) is often treated with invasive surgery and fixed-size implants to support the trachea during respiration. A novel 3D-printed extra-luminal splint has been developed as a flexible and bioresorbable alternative. Therefore, the goal of the present study was to use an in vitro breathing simulator model to comprehensively evaluate the structural stiffness and failure modes of two sizes of a novel bioresorbable 3D-printed splint design under a range of physiological degradation conditions. Two thicknesses, 2 mm and 3 mm, of a novel 3D-printed bioresorbable splint were evaluated under two different degradation conditions, phosphate-buffered saline (PBS) and sodium hydroxide (NaOH). The splints were subjected to simulated breathing loading, involving a cyclic opening and closing of the splint by 2 mm, for a targeted duration of 7.5 to 30 million cycles. A separate new set of splints were statically soaked in their respective degradation condition for a comparative analysis of the effects of cyclic loading by the degradation medium. After successfully simulated breathing or static soaking, non-destructive tensile and compressive strengths were evaluated, and overall stiffness was calculated from destructive tensile testing. The present study indicates that the splints were more significantly degraded under simulated breathing conditions than under soaking. Cyclic simulated breathing specimens failed far earlier than the intended duration of loading. Over time, both 2 mm and 3 mm splints became increasingly more flexible when subjected to the static degradation conditions. Interestingly, there was little difference in the compressive and tensile strengths of the 2 mm and 3 mm thickness splints. The bioresorbable nature of PCL offers a valuable advantage as it eliminates the need for splint removal surgery and increases device flexibility over time with degradation. This increased flexibility is crucial because it allows for uninhibited growth and development of the infant's trachea over the intended use period of 2 years. The results of this study confirm that the splints were able to withstand tensile forces to prevent tracheal collapse. This study further supports the successful use of 3D-printed splints in the treatment of infantile TBM.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbm.b.35501","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An In Vivo Assessment of Different Mesenchymal Stromal Cell Tissue Types and Their Differentiation State on a Shape Memory Polymer Scaffold for Bone Regeneration","authors":"Teja Guda,&nbsp;Jessica M. Stukel Shah,&nbsp;Bridney D. Lundquist,&nbsp;Joseph M. Macaitis,&nbsp;Mística Lozano Pérez,&nbsp;Michaela R. Pfau-Cloud,&nbsp;Felipe O. Beltran,&nbsp;Connie W. Schmitt,&nbsp;Emily M. Corbin,&nbsp;Melissa A. Grunlan,&nbsp;Wen Lien,&nbsp;Heuy-Ching Wang,&nbsp;Alexander J. Burdette","doi":"10.1002/jbm.b.35516","DOIUrl":"10.1002/jbm.b.35516","url":null,"abstract":"<div>\u0000 \u0000 <p>A combined biomaterial and cell-based solution to heal critical size bone defects in the craniomaxillofacial area is a promising alternative therapeutic option to improve upon autografting, the current gold standard. A shape memory polymer (SMP) scaffold, composed of biodegradable poly(ε-caprolactone) and coated with bioactive polydopamine, was evaluated with mesenchymal stromal cells (MSCs) derived from adipose (ADSC), bone marrow (BMSC), or umbilical cord (UCSC) tissue in their undifferentiated state or pre-differentiated toward osteoblasts for bone healing in a rat calvarial defect model. Pre-differentiating ADSCs and UCSCs resulted in higher new bone volume fraction (15.69% ± 1.64%) compared to empty (i.e., untreated) defects and scaffold-only (i.e., unseeded) groups (4.41% ± 1.11%). Notably, only differentiated UCSCs exhibited a significant increase in new bone volume, surpassing both undifferentiated UCSCs and unseeded scaffolds. Further, differentiated ADSCs and UCSCs had significantly higher trabecular numbers than their undifferentiated counterparts, unseeded scaffolds, and untreated defects. Although the mineral density regenerated within the unseeded scaffold surpassed that achieved with cell seeding, the connectivity of this bone was diminished, as the regenerated tissue confined itself to the spherical morphology of the scaffold pores. The SMP scaffold alone, with undifferentiated BMSCs, with undifferentiated and differentiated ADSCs, and differentiated UCSCs (29.72 ± 1.49 N) demonstrated significant osseointegration compared to empty defects (14.34 ± 2.21 N) after 12 weeks of healing when assessed by mechanical push-out testing. Based on these results and tissue availability to obtain the cells, pre-differentiated ADSCs and UCSCs emerge as particularly promising candidates when paired with the SMP scaffold for repairing critical size bone defects in the craniofacial skeleton.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shape Memory Polymer Scaffolds—Utility for In Vitro Osteogenesis of Canine Multipotent Stromal Cells","authors":"Shelby B. Gasson,&nbsp;Lauren K. Dobson,&nbsp;Michaela R. Pfau-Cloud,&nbsp;Felipe O. Beltran,&nbsp;Carl A. Gregory,&nbsp;Melissa A. Grunlan,&nbsp;W. Brian Saunders","doi":"10.1002/jbm.b.35503","DOIUrl":"10.1002/jbm.b.35503","url":null,"abstract":"<div>\u0000 \u0000 <p>A biodegradable, shape memory polymer (SMP) scaffold based on poly(ε-caprolactone) (PCL) represents an attractive alternative therapy for the repair of critically sized bone defects given its ability to press-fit within irregular defects. Clinical translation of SMP scaffolds requires successful movement beyond proof-of-concept rodent studies through a relevant large-animal model and into the clinical setting. In addition to representing a clinical veterinary population, the canine species is a strong translational model for humans due to similarities in orthopedic disorders, biomechanics, and bone healing. The present study was performed to assess in vitro cytocompatibility and osteogenic differentiation of canine multipotent stromal cells (cMSCs) cultured on SMP scaffolds in preparation for future canine in vivo studies. Two different SMP scaffold compositions were utilized: a “PCL-only” scaffold prepared from PCL-diacrylate (PCL-DA) and a semi-interpenetrating network (semi-IPN) formed from PCL-DA and poly(L-lactic acid) (PCL:PLLA). The PCL:PLLA scaffolds degrade faster and are more mechanically rigid versus the PCL scaffolds. Canine bone marrow–derived MSCs (cMSCs) were evaluated in terms of attachment, proliferation, and osteogenic differentiation. cMSCs exhibited excellent cytocompatibility, attachment, and proliferation on both SMP scaffold compositions. PCL scaffolds were more conducive to both early- and late-stage in vitro osteogenesis of cMSCs versus PCL:PLLA scaffolds. However, cMSCs deposited mineralized extracellular matrix over 21 days when cultured on both SMP scaffold compositions. These results demonstrate that the SMP scaffolds are suitable for in vitro cMSC attachment, proliferation, and osteogenic differentiation, representing a significant step toward canine in vivo studies and potential translation to human patients.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melt-Blown Polypropylene Membrane Modification for Enhanced Hydrophilicity","authors":"M. Lam,&nbsp;M. Baudoin,&nbsp;B. Mougin,&nbsp;C. Falentin-Daudré","doi":"10.1002/jbm.b.35509","DOIUrl":"10.1002/jbm.b.35509","url":null,"abstract":"<div>\u0000 \u0000 <p>Melt-blown, an environmentally friendly technique, is widely used to create high-quality non-woven fabrics by extruding molten polymer resins into interlaced fibers. In the realm of biomedical textiles, its unique microstructure makes it ideal for filtration and wound dressings. Our study focuses on modifying the surfaces of polypropylene melt-blown membranes. An effective, one-step, suitable, and reliable method to graft a bioactive polymer, sodium polystyrene sulfonate—PolyNaSS, onto the membranes has been developed. The process involves UV irradiation to initiate direct and progressive growth of NaSS over the surface through radical polymerization. To assess the efficiency of the grafting, techniques like colorimetry, water contact angle measurements, Fourier-transformed infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) were used. Outcomes related to the grafting were demonstrated by a change in wettability and quantitatively calculated sulfonate groups. Subsequently, grafted PolyNaSS promoted cell adhesion, as evidenced by improved cell morphology. On grafted membranes, fibroblasts exhibited a stretched shape, while non-grafted ones showed inactive round shapes. These findings underscore the chemical and biological reactivity of polypropylene materials, opening exciting possibilities for various applications of melt-blown materials.</p>\u0000 </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"112 12","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}