Versatile Biomaterial Additive: A Game-Changing Multifunctional Synthetic Peptide With Pro-Regenerative, Anti-Inflammatory, and Antibacterial Properties

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL
Mirosława Panasiuk, Milena Chraniuk, Piotr Bollin, Justyna Sawicka, Anna Sylla, Lilit Hovhannisyan, Monika Biernat, Sylwia Rodziewicz-Motowidło, Beata Gromadzka
{"title":"Versatile Biomaterial Additive: A Game-Changing Multifunctional Synthetic Peptide With Pro-Regenerative, Anti-Inflammatory, and Antibacterial Properties","authors":"Mirosława Panasiuk,&nbsp;Milena Chraniuk,&nbsp;Piotr Bollin,&nbsp;Justyna Sawicka,&nbsp;Anna Sylla,&nbsp;Lilit Hovhannisyan,&nbsp;Monika Biernat,&nbsp;Sylwia Rodziewicz-Motowidło,&nbsp;Beata Gromadzka","doi":"10.1002/jbm.b.35591","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The prevalence of bone disorders and deformities is increasing due to trauma, malignant neoplasms, infections, and degenerative and inflammatory diseases. Bone repair and replacement have evolved as a consequence of advancements in orthopedic technology and biomaterials with enhanced properties. The rapid growth of the bone tissue engineering field is being significantly influenced by biomaterials, such as polymer scaffolds with appropriate surface modifications. New additives are constantly being developed in response to the increasing demand for enhancing the bioactivity of biocomposites used for bone regeneration. We present the design and synthesis of a synthetic bioactive peptide UG46, which is multifunctional and consists of the fragment of human Cystatin C (CystC) and anoplin. In addition, the peptide is assessed as an additive that is employed to enhance the repair of bone by enriching porous chitosan (CH) scaffolds. Our results indicated that the UG46 peptide possesses pro-regenerative properties, while it did not exhibit any cytotoxic effects on human osteoblasts or human fibroblasts. Incubation of cells with CH and CH-UG46 extracts significantly increased cell proliferation, cell proliferation over 200% of control cells. Even though the proliferation assay revealed a significant inhibition of cell proliferation in cells seeded directly on the composites, the beneficial effect of the UG46 peptide was still noticeable. Additionally, the UG46 peptide exhibited dose-dependent anti-inflammatory properties in both its free form and as a biocomposite additive, qualifying it as a promising candidate for a bone biomaterial component. The working concentrations of UG46 peptide have been established at 40–80 μg/mL. The molecular structure analysis of the CH-UG46 biocomposites revealed that the majority of the pores were sufficiently large to enable osteoblast cells to infiltrate the scaffold, while concurrent microporosity (&lt; 20 μm) enabled cell infiltration, vascularization, and cell-matrix interactions. Additionally, the peptide alone exhibits limited antibacterial properties; however, the peptide released from the CH-UG46 biocomposite at high levels has been demonstrated to be capable of inhibiting the growth of the selected bacterial strains that are most frequently found infecting healing wounds by over 83% for all strains tested stains with A. baumani bacterial count reduction at 99.86%. Our findings indicate that the bioactive peptide we have proposed is a promising enhancement for porous scaffolds. It has the potential to facilitate the creation of specialized, custom-designed biomaterials with multifunctional properties, including healing, defense, and hygiene.</p>\n </div>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":"113 5","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical materials research. Part B, Applied biomaterials","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.35591","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0

Abstract

The prevalence of bone disorders and deformities is increasing due to trauma, malignant neoplasms, infections, and degenerative and inflammatory diseases. Bone repair and replacement have evolved as a consequence of advancements in orthopedic technology and biomaterials with enhanced properties. The rapid growth of the bone tissue engineering field is being significantly influenced by biomaterials, such as polymer scaffolds with appropriate surface modifications. New additives are constantly being developed in response to the increasing demand for enhancing the bioactivity of biocomposites used for bone regeneration. We present the design and synthesis of a synthetic bioactive peptide UG46, which is multifunctional and consists of the fragment of human Cystatin C (CystC) and anoplin. In addition, the peptide is assessed as an additive that is employed to enhance the repair of bone by enriching porous chitosan (CH) scaffolds. Our results indicated that the UG46 peptide possesses pro-regenerative properties, while it did not exhibit any cytotoxic effects on human osteoblasts or human fibroblasts. Incubation of cells with CH and CH-UG46 extracts significantly increased cell proliferation, cell proliferation over 200% of control cells. Even though the proliferation assay revealed a significant inhibition of cell proliferation in cells seeded directly on the composites, the beneficial effect of the UG46 peptide was still noticeable. Additionally, the UG46 peptide exhibited dose-dependent anti-inflammatory properties in both its free form and as a biocomposite additive, qualifying it as a promising candidate for a bone biomaterial component. The working concentrations of UG46 peptide have been established at 40–80 μg/mL. The molecular structure analysis of the CH-UG46 biocomposites revealed that the majority of the pores were sufficiently large to enable osteoblast cells to infiltrate the scaffold, while concurrent microporosity (< 20 μm) enabled cell infiltration, vascularization, and cell-matrix interactions. Additionally, the peptide alone exhibits limited antibacterial properties; however, the peptide released from the CH-UG46 biocomposite at high levels has been demonstrated to be capable of inhibiting the growth of the selected bacterial strains that are most frequently found infecting healing wounds by over 83% for all strains tested stains with A. baumani bacterial count reduction at 99.86%. Our findings indicate that the bioactive peptide we have proposed is a promising enhancement for porous scaffolds. It has the potential to facilitate the creation of specialized, custom-designed biomaterials with multifunctional properties, including healing, defense, and hygiene.

多功能生物材料添加剂:一种改变游戏规则的多功能合成肽,具有促再生、抗炎和抗菌特性
由于创伤、恶性肿瘤、感染以及退行性和炎症性疾病,骨疾病和畸形的患病率正在增加。随着骨科技术的进步和生物材料性能的提高,骨修复和骨置换技术也在不断发展。骨组织工程领域的快速发展受到生物材料的显著影响,例如具有适当表面修饰的聚合物支架。新的添加剂不断开发,以响应日益增长的需求,提高生物复合材料的生物活性用于骨再生。我们设计并合成了一种多功能的合成生物活性肽UG46,它由人类胱抑素C (CystC)片段和anoplin组成。此外,该肽被评估为一种添加剂,用于通过丰富多孔壳聚糖(CH)支架来增强骨修复。我们的研究结果表明,UG46肽具有促进再生的特性,而它对人成骨细胞或人成纤维细胞没有任何细胞毒性作用。CH和CH- ug46提取物孵育细胞后,细胞增殖能力显著提高,细胞增殖能力超过对照细胞的200%。尽管增殖实验显示直接接种在复合材料上的细胞对细胞增殖有明显的抑制作用,但UG46肽的有益作用仍然是明显的。此外,UG46肽在其自由形态和作为生物复合添加剂时均表现出剂量依赖性抗炎特性,使其成为骨生物材料成分的有希望的候选物。UG46肽的工作浓度为40 ~ 80 μg/mL。CH-UG46生物复合材料的分子结构分析表明,大多数孔隙足够大,可以使成骨细胞浸润支架,而同时存在的微孔隙(< 20 μm)可以使细胞浸润、血管形成和细胞-基质相互作用。此外,肽单独显示有限的抗菌性能;然而,从CH-UG46生物复合材料中释放的高水平肽已被证明能够抑制最常感染愈合伤口的特定细菌菌株的生长,在所测试的菌株中,所有菌株的细菌计数减少99.86%,抑制率超过83%。我们的研究结果表明,我们提出的生物活性肽是一种有希望的多孔支架增强剂。它有可能促进专门的、定制设计的生物材料的创造,这些材料具有多种功能,包括愈合、防御和卫生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信