Journal of biochemistry最新文献_第2页

Correction to: Innate immune signals triggered on organelle membranes. 修正：先天免疫信号触发细胞器膜。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-24 DOI: 10.1093/jb/mvaf035

引用次数: 0

Transcriptional control of brown adipocyte differentiation and function by NFIA: recent perspectives on deciphering metabolic diseases. NFIA对棕色脂肪细胞分化和功能的转录控制：解读代谢疾病的最新观点。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-23 DOI: 10.1093/jb/mvaf038

Yuta Hiraike

引用次数: 0

Mitochondrial DNA: leakage, recognition, and associated human diseases. 线粒体DNA：泄漏、识别和相关的人类疾病。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-20 DOI: 10.1093/jb/mvaf037

Hyota Takamatsu

引用次数: 0

Crystal structure of Klebsiella pneumoniae maltohexaose-producing α-amylase. 肺炎克雷伯菌产麦芽己糖α-淀粉酶的晶体结构。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-18 DOI: 10.1093/jb/mvaf034

Zui Fujimoto, Naomi Kishine, Mitsuru Momma

引用次数: 0

Maintaining a neutral range disperses myosin molecules under salt-free conditions. 在无盐条件下保持中性范围分散肌球蛋白分子。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-18 DOI: 10.1093/jb/mvaf036

Toru Hayakawa, Yu Shishido, Yuki Ikeuchi, Jun-Ichi Wakamatsu, Haruto Kumura

{"title":"Maintaining a neutral range disperses myosin molecules under salt-free conditions.","authors":"Toru Hayakawa, Yu Shishido, Yuki Ikeuchi, Jun-Ichi Wakamatsu, Haruto Kumura","doi":"10.1093/jb/mvaf036","DOIUrl":"https://doi.org/10.1093/jb/mvaf036","url":null,"abstract":"Skeletal muscle myosin is generally considered insoluble under physiological, low ionic strength, or salt-free conditions due to its tendency to self-assemble into filamentous polymers in vitro. Our previous study showed that myosin can be solubilized in low ionic strength solutions containing l-histidine. However, another report suggested that 1-methylhistidine could not solubilize myosin, and the factors essential for myosin solubilization remain unclear. To elucidate the role of l-histidine in the water solubilization of myosin, we examined myosin solubility and the molecular properties of its rod domain, l-meromyosin, using structurally related buffer compounds. Under salt-free conditions, solubility depended heavily on the acid dissociation constant of buffer, indicating that maintaining a neutral pH is critical. The rod domain showed molecular elongation regardless of the buffer type, yet surface charge and hydrophobicity remained comparable to conditions with high ionic strength. These results suggest that myosin is inherently soluble and maintains its structural integrity under neutral, salt-free conditions. The apparent insolubility under such conditions is likely to result from hydrochloric acid used for pH adjustment. Since l-histidine and imidazole achieve neutrality without acid addition, they are ideal buffers for myosin solubilization.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription and translation efficiency is reduced in cholesterol-containing liposomes. 在含胆固醇的脂质体中，转录和翻译效率降低。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-13 DOI: 10.1093/jb/mvaf032

Shota Fukuoka, Ayu Shimomura, Yuya Katsumura, Masaya Oki, Gakushi Tsuji

引用次数: 0

Bisecting GlcNAc modification of angiotensin-related glycoproteins in mouse kidney. GlcNAc对小鼠肾脏血管紧张素相关糖蛋白的修饰。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-13 DOI: 10.1093/jb/mvaf033

Haruka Kawade, Wanxue Bao, Yuko Tokoro, Yoshimasa Ito, Yudai Tsuji, Kazuo Takahashi, Kazuki Nakajima, Miyako Nakano, Yasuhiko Kizuka

{"title":"Bisecting GlcNAc modification of angiotensin-related glycoproteins in mouse kidney.","authors":"Haruka Kawade, Wanxue Bao, Yuko Tokoro, Yoshimasa Ito, Yudai Tsuji, Kazuo Takahashi, Kazuki Nakajima, Miyako Nakano, Yasuhiko Kizuka","doi":"10.1093/jb/mvaf033","DOIUrl":"https://doi.org/10.1093/jb/mvaf033","url":null,"abstract":"Structural variations of N-glycans critically regulate glycoprotein functions and are involved in various human diseases. N-Acetylglucosaminyltransferase-III (GnT-III or MGAT3) is highly expressed in the brain and kidney and is an N-glycan branching enzyme that biosynthesizes the unique N-glycan branch designated as bisecting GlcNAc. Its roles in Alzheimer's disease and cancer have been revealed, but the functions of bisecting GlcNAc in the kidney are poorly understood. Here, we show that kidneys in the GnT-III-knockout (KO) mouse exhibit impaired body fluid balance and present interstitial edema. To understand the molecular mechanisms further, we biochemically purified the glycoproteins modified by GnT-III in the mouse kidney and identified these proteins using proteomics. We found that the proteins involved in the pathway for angiotensin II (Ang II) metabolism are modified by GnT-III, and that the subcellular localization of angiotensin converting enzyme was altered in GnT-III-KO cells. Furthermore, the pathology in models of Ang II-related disease was slightly more severe in GnT-III-KO than in wild-type mice. Our data indicate a protective role for bisecting GlcNAc in the mouse kidney, highlighting a newly described link between specific N-glycan structures and renal functions.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential regulation between photosynthetic type and non-photosynthetic type Fd:FNRs in the negative cooperativity and pH dependency of the electron transfer activity. 光合型与非光合型FNRs对电子转移活性负协同性和pH依赖性的差异调控。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-10 DOI: 10.1093/jb/mvaf031

Yoko Kimata-Ariga, Shunsuke Miyake, Takato Murakami, Shunsuke Kuwano

{"title":"Differential regulation between photosynthetic type and non-photosynthetic type Fd:FNRs in the negative cooperativity and pH dependency of the electron transfer activity.","authors":"Yoko Kimata-Ariga, Shunsuke Miyake, Takato Murakami, Shunsuke Kuwano","doi":"10.1093/jb/mvaf031","DOIUrl":"https://doi.org/10.1093/jb/mvaf031","url":null,"abstract":"In higher plants, ferredoxin (Fd) and Fd-NADP+ reductase (FNR) are each present as distinct isoproteins of photosynthetic type and non-photosynthetic type, which exhibit differential function despite their similarity in the 3D structures. In this study, we addressed differential regulation of Fd/FNR reaction between the two types from two perspectives and investigated the amino acid residues of Fd responsible for the differences. Firstly, pH-dependent profile of Fd/FNR electron transfer activity varied among the combinations of the two types of Fd and FNR; non-photosynthetic type FNR showed similar pattern for the two types of Fds while photosynthetic type FNR was previously shown to exhibit opposite pattern which was explained by the different pH-dependent profile of Km for the two Fds. Secondly, the extent of the suppression of the affinity (in terms of Km value) between Fd and FNR by NADPH significantly varied among the combinations of the two types of Fd:FNR. The difference was shown to be mainly due to the different property of Fd between the two types. Kinetic analyses using site-directed mutants of Fd showed the contribution of C-terminal residues, together with that of 78th residue of Fd, on the differential profile of Fd/FNR reaction by pH and NADPH.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three glutamic acid residues in the cytoplasmic N-terminal tail of long-form GlcAT-P define Golgi-to-ER trafficking. 长形GlcAT-P的细胞质n端尾部的三个谷氨酸残基定义了高尔基到内质网的运输。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-06-09 DOI: 10.1093/jb/mvaf030

Ayaka Okada, Risa Harui, Tomonari Ishida, Katsuaki Higashi, Motohiro Nonaka, Shogo Oka, Jyoji Morise

{"title":"Three glutamic acid residues in the cytoplasmic N-terminal tail of long-form GlcAT-P define Golgi-to-ER trafficking.","authors":"Ayaka Okada, Risa Harui, Tomonari Ishida, Katsuaki Higashi, Motohiro Nonaka, Shogo Oka, Jyoji Morise","doi":"10.1093/jb/mvaf030","DOIUrl":"https://doi.org/10.1093/jb/mvaf030","url":null,"abstract":"Glucuronyltransferase GlcAT-P is a rate-limiting enzyme involved in the biosynthesis of the Human Natural Killer-1 carbohydrate and is essential for acquiring higher brain functions. Alternative splicing produces two isoforms, short-form GlcAT-P (sGlcAT-P) and long-form GlcAT-P (lGlcAT-P), which share identical peptide sequences except for an additional 13 amino acids (AA) in the cytoplasmic N-terminal tail of lGlcAT-P. Although sGlcAT-P localizes to the Golgi apparatus (GA), where many glycosyltransferases reside, lGlcAT-P is distributed in both the GA and endoplasmic reticulum (ER). However, the mechanisms responsible for this distinct intracellular distribution remain poorly understood. In this study, we explored the role of the 13 AA in the cytoplasmic N-tail of lGlcAT-P in trafficking between the GA and the ER using the Retention Using Selective Hooks system. Our findings revealed that lGlcAT-P undergoes enhanced retrograde trafficking from the GA to the ER, whereas its anterograde trafficking from the ER to the GA remains largely unaffected. In addition, three glutamic acid residues within the 13 AA of lGlcAT-P were identified as crucial for promoting retrograde trafficking. These results suggest that the ER distribution of lGlcAT-P is primarily governed by Golgi-to-ER trafficking regulated by specific sequences in its cytoplasmic N-tail.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suicide substrate reaction-like modification of mouse serine racemase with L-serine. l -丝氨酸修饰小鼠丝氨酸消旋酶的自杀底物样反应。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-05-30 DOI: 10.1093/jb/mvaf019

Akari Hata, Tomokazu Ito, Hitoshi Mori, Takuya Ogawa, Tatsuo Kurihara, Hisashi Hemmi, Tohru Yoshimura

{"title":"Suicide substrate reaction-like modification of mouse serine racemase with L-serine.","authors":"Akari Hata, Tomokazu Ito, Hitoshi Mori, Takuya Ogawa, Tatsuo Kurihara, Hisashi Hemmi, Tohru Yoshimura","doi":"10.1093/jb/mvaf019","DOIUrl":"10.1093/jb/mvaf019","url":null,"abstract":"A pyridoxal 5'-phosphate-dependent fold-type II serine racemase (SR) is responsible for the synthesis of D-Ser, which serves as a co-agonist of N-methyl-D-aspartate glutamate receptor. In addition to racemization, SR catalyzes the dehydration of D- and L-Ser. SR is suggested to be involved in the D-Ser degradation in vivo, but this has not been confirmed. In this study, we found that mouse SR (mSR) underwent a suicide substrate reaction-like modification with its substrate, resulting in a remarkable change in its reaction specificity. mSR gradually lost its activity by the incubation with L- and D-Ser, but not completely. mSR was labelled with [14C]-L-Ser. ESI-MS analysis revealed that the molecular mass of SR increased by 84 Da by the incubation with L-Ser. Taken together with the results of previous crystallographic studies of fission yeast SR, we concluded that the active site lysine residue of mSR was modified with an α-aminoacrylate intermediate generated from L-Ser and converted to a lysinoalanine residue. The modification significantly decreased the racemization and L-Ser dehydration activities, while dramatically increased the D-Ser dehydration activity by the ~100 times reduction of the Km value. This is probably advantageous for the D-Ser degradation by mSR under physiological conditions.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"437-445"},"PeriodicalIF":2.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0