Journal of biochemistry最新文献_第10页

Mitochondrial lipid dynamics regulated by MITOL-mediated ubiquitination. 线粒体脂质动态受 MITOL 介导的泛素化调控。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad117

Koji Yamano, Hiroki Kinefuchi, Waka Kojima

引用次数: 0

Dephosphorylation of NFAT by Calcineurin inhibits Skp2-mediated degradation. 钙调磷酸酶对NFAT的去磷酸化抑制skp2介导的降解。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad103

Shunsuke Hanaki, Makoto Habara, Yuki Sato, Haruki Tomiyasu, Yosei Miki, Shusaku Shibutani, Midori Shimada

{"title":"Dephosphorylation of NFAT by Calcineurin inhibits Skp2-mediated degradation.","authors":"Shunsuke Hanaki, Makoto Habara, Yuki Sato, Haruki Tomiyasu, Yosei Miki, Shusaku Shibutani, Midori Shimada","doi":"10.1093/jb/mvad103","DOIUrl":"10.1093/jb/mvad103","url":null,"abstract":"The transcription factor NFAT plays key roles in multiple biological activities, such as immune responses, tissue development and malignant transformation. NFAT is dephosphorylated by calcineurin, which is activated by intracellular calcium levels, and translocated into the nucleus, resulting in transcriptional activation. Calcineurin dephosphorylates various target proteins and regulates their functions. However, the regulation of NFAT degradation is largely unknown, and it is unclear whether calcineurin contributes to the stability of NFAT. We investigated the effect of calcineurin inhibition on NFAT protein stability and found that the dephosphorylation of NFAT by calcineurin promotes the NFAT stabilization, whereas calcineurin mutant that is defective in phosphatase activity was unable to stabilize NFAT. Increased intracellular calcium ion concentration, which is essential for calcineurin activation, also induced NFAT stability. In addition, we identified S-phase kinase associated protein 2 (Skp2), an F-box protein of the SCF ubiquitin ligase complex, as a factor mediating degradation of NFAT when calcineurin was depleted. In summary, these findings revealed that the dephosphorylation of NFAT by calcineurin protects NFAT from degradation by Skp2 and promotes its protein stability.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"235-244"},"PeriodicalIF":2.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138460129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An oscillating magnetic field suppresses ice-crystal growth during rapid freezing of muscle tissue of mice. 在小鼠肌肉组织的快速冷冻过程中，振荡磁场抑制冰晶生长。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad087

Kana Okuda, Kunitani Kaori, Aiko Kawauchi, Ishii Miyu, Kentaro Yomogida

{"title":"An oscillating magnetic field suppresses ice-crystal growth during rapid freezing of muscle tissue of mice.","authors":"Kana Okuda, Kunitani Kaori, Aiko Kawauchi, Ishii Miyu, Kentaro Yomogida","doi":"10.1093/jb/mvad087","DOIUrl":"10.1093/jb/mvad087","url":null,"abstract":"Regenerative medicine would benefit from a safe and efficient cryopreservation method to prevent the structural disruption caused by ice-crystal formation in cells and tissue. Various attempts have been made to overcome this problem, one of which is the use of an oscillating magnetic field (OMF). However, the underlying mechanism is unclear. In this study, to evaluate the effect of an OMF on ice-crystal formation in the leg muscles of mice, we used to use the frozen-section method with a slower freezing rate than is, usual which resulted in ice crystals forming in the tissue. We assessed the mean size and number per unit area of intracellular ice holes in sections of muscle tissue, with and without OMF. Ice-crystal growth was reduced in frozen tissue subjected to OMF. Furthermore, we evaluated the structure and function of proteins in frozen tissue subjected to OMF by immunostaining using an anti-dystrophin antibody and by enzymatic histochemistry for NADH-TR and myosin ATPase. The results imply that the ability of OMF to suppress ice-crystal growth might be related to their stabilization of bound water in biomolecules during freezing.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"245-252"},"PeriodicalIF":2.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72209440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Overexpression of Sall1 in vivo leads to reduced body weightwithout affecting kidney development. 更正为体内过表达 Sall1 会导致体重减轻，但不影响肾脏发育。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad108

引用次数: 0

NRIP1 regulates cell proliferation in lung adenocarcinoma cells. NRIP1 可调节肺腺癌细胞的增殖。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad107

Fumihiko Watanabe, Shigemitsu Sato, Takuo Hirose, Moe Endo, Akari Endo, Hiroki Ito, Koji Ohba, Takefumi Mori, Kazuhiro Takahashi

{"title":"NRIP1 regulates cell proliferation in lung adenocarcinoma cells.","authors":"Fumihiko Watanabe, Shigemitsu Sato, Takuo Hirose, Moe Endo, Akari Endo, Hiroki Ito, Koji Ohba, Takefumi Mori, Kazuhiro Takahashi","doi":"10.1093/jb/mvad107","DOIUrl":"10.1093/jb/mvad107","url":null,"abstract":"Nuclear receptor interacting protein 1 (NRIP1) is a transcription cofactor that regulates the activity of nuclear receptors and transcription factors. Functional expression of NRIP1 has been identified in multiple cancers. However, the expression and function of NRIP1 in lung adenocarcinoma have remained unclear. Thus, we aimed to clarify the NRIP1 expression and its functions in lung adenocarcinoma cells. NRIP1 and Ki-67 were immunostained in the tissue microarray section consisting of 64 lung adenocarcinoma cases, and the association of NRIP1 immunoreactivity with clinical phenotypes was examined. Survival analysis was performed in lung adenocarcinoma data from The Cancer Genome Atlas (TCGA). Human A549 lung adenocarcinoma cell line with an NRIP1-silencing technique was used in vitro study. Forty-three of 64 cases were immunostained with NRIP1. Ki-67-positive cases were more frequent in NRIP1-positive cases as opposed to NRIP1-negative cases. Higher NRIP1 mRNA expression was associated with poor prognosis in the TCGA lung adenocarcinoma data. NRIP1 was mainly located in the nucleus of A549 cells. NRIP1 silencing significantly reduced the number of living cells, suppressed cell proliferation, and induced apoptosis. These results suggest that NRIP1 participates in the progression and development of lung adenocarcinoma. Targeting NRIP1 may be a possible therapeutic strategy against lung adenocarcinoma.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"323-333"},"PeriodicalIF":2.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138802039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel synthetic biological study on intracellular distribution of human GlcNAc-1-phosphotransferase expressed in insect cells. 昆虫细胞中表达的人GlcNAc-1-磷酸转移酶细胞内分布的新合成生物学研究。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad090

Kei Kiriyama, Keisuke Fujioka, Kaito Kawai, Teru Mizuno, Yasuo Shinohara, Kohji Itoh

{"title":"Novel synthetic biological study on intracellular distribution of human GlcNAc-1-phosphotransferase expressed in insect cells.","authors":"Kei Kiriyama, Keisuke Fujioka, Kaito Kawai, Teru Mizuno, Yasuo Shinohara, Kohji Itoh","doi":"10.1093/jb/mvad090","DOIUrl":"10.1093/jb/mvad090","url":null,"abstract":"Many lysosomal enzymes contain N-glycans carrying mannose 6-phosphate (M6P) residues. Modifying lysosomal enzymes by M6P residues requires a two-step process in the Golgi apparatus. Then the lysosomal enzymes with M6P residues are transported from the trans-Golgi network to endosomes and lysosomes by M6P receptors. In insect cells, M6P residues are not added to N-glycans. Therefore, many insect lysosomal enzymes are transported to lysosomes by the M6P-independent pathway. The expression and subcellular distribution of M6P-modifying enzymes were examined by amplifying DNA fragments of M6P-modifying enzymes, generating the corresponding plasmid constructs, and transfection each construct into Sf9 cells, an insect cell line. The human GlcNac-1-phosphotransferase α/β subunit, one of the M6P-modifying enzymes, was found to differ in maturation and localization between mammalian and insect cells. In mammalian cells, newly biosynthesized α/β subunit localized in the cis-Golgi. In Sf9 cells, most of the α/β subunit was localized in the endoplasmic reticulum, and few mature forms of α/β subunit were observed. However, by the co-expression of the human site-1 protease, the mature forms were observed significantly and co-localization with each protein. Our study indicates new insights into regulating the intracellular distribution of the human GlcNac-1-phosphotransferase α/β subunit in insect cells.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"265-274"},"PeriodicalIF":2.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72209450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Merlin/NF2 regulates SLC7A11/xCT expression and cell viability under glucose deprivation at high cell density in glioblastoma cells. Merlin/NF2调控胶质母细胞瘤细胞在高密度细胞葡萄糖剥夺条件下的SLC7A11/xCT表达和细胞活力。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad105

Itsuki Yamaguchi, Hironori Katoh

引用次数: 0

Comparative study of the steady-state subcellular distribution of lysosome-associated membrane glycoprotein-2 (LAMP-2) isoforms with GYXXΦ-type tyrosine-based motifs that interact differently with four adaptor protein (AP) complexes. 溶酶体相关膜糖蛋白-2 (LAMP-2)亚型与GYXXΦ-type酪氨酸基序的稳态亚细胞分布的比较研究，这些基序与四种接头蛋白(AP)复合物的相互作用不同。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad096

Fumiaki Yamaguchi, Hiroshi Sakane, Kenji Akasaki

{"title":"Comparative study of the steady-state subcellular distribution of lysosome-associated membrane glycoprotein-2 (LAMP-2) isoforms with GYXXΦ-type tyrosine-based motifs that interact differently with four adaptor protein (AP) complexes.","authors":"Fumiaki Yamaguchi, Hiroshi Sakane, Kenji Akasaki","doi":"10.1093/jb/mvad096","DOIUrl":"10.1093/jb/mvad096","url":null,"abstract":"Lysosome-associated membrane protein-1 and -2 (LAMP-1 and LAMP-2, respectively) are type I transmembrane proteins. LAMP-2 comprises three splice isoforms (LAMP-2A, -B and-C) with different cytoplasmic tails (CTs). These three CTs possess different tyrosine-based motifs (GYXXΦ, where Φ is a bulky hydrophobic amino acid) at their C-termini. Interactions between tyrosine-based motifs and μ-subunits of four tetrameric adaptor protein (AP) complexes are necessary for their vesicular transport to lysosomes. Little is known about how the interaction strengths of these tyrosine motifs with μ-subunits affect the localization of isoforms to lysosomes. The interactions were first investigated using a yeast two-hybrid system to address this question. LAMP-2A-CT interacted with all four μ-subunits (μ1, μ2, μ3A and μ4 of AP-1, AP-2, AP-3 and AP-4, respectively). The interaction with μ3A was more robust than that with other μ-subunits. LAMP-2B-CT interacted exclusively and moderately with μ3A. LAMP-2C-CT did not detectably interact with any of the four μ-subunits. Immunofluorescence microscopy showed that all isoforms were localized in late endosomes and lysosomes. LAMP-2C was present in the plasma membrane and early endosomes; however, LAMP-2A and -2B were barely detectable in these organelles. In cell fractionation, LAMP-2A was the most abundant in the dense lysosomes, whereas LAMP-2C was significantly present in the low-density fraction containing the plasma membrane and early endosomes, in addition to the dense lysosomes. LAMP-2B considerably existed in the low-density late endosomal fraction. These data strongly suggest that the LAMP-2 isoforms are distributed differently in endocytic organelles depending on their interaction strengths with AP-3.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"275-287"},"PeriodicalIF":2.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The incorporation of extracellular vesicle markers varies among vesicles with distinct surface charges. 细胞外囊泡标记物的结合在具有不同表面电荷的囊泡中是不同的。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad097

Koki Maeda, Simon Goto, Koya Miura, Koki Saito, Eiji Morita

{"title":"The incorporation of extracellular vesicle markers varies among vesicles with distinct surface charges.","authors":"Koki Maeda, Simon Goto, Koya Miura, Koki Saito, Eiji Morita","doi":"10.1093/jb/mvad097","DOIUrl":"10.1093/jb/mvad097","url":null,"abstract":"Extracellular vesicles (EVs) are important mediators of intercellular communication. However, the methods available for distinguishing the heterogeneity of secreted EVs and isolating and purifying them are limited. This study introduced a HiBiT-tag to detect various EV markers, including CD63, CD9, Epidermal Growth Factor Receptor (EGFR), Flotilin1, and Syndecan-1, and investigated whether these marker-containing vesicles were capable of binding to differently charged column carriers. Four column carriers, Diethylaminoethyl (DEAE), Capto Adhere, Blue and Heparin, showed affinity for CD63 containing EVs, but their elution patterns varied. Furthermore, we observed that the elution patterns of the EV markers differed among vesicles with distinct surface charges when a DEAE column was used. This suggests that the incorporation of EV markers varied between these vesicles. The markers showed different subcellular localizations, indicating that the site of vesicle formation may contribute to the production of vesicles with varying charges and marker incorporation. These findings may have implications for the development of methods to purify homogeneous EVs, which could be useful in EV-mediated drug delivery systems.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"299-312"},"PeriodicalIF":2.7,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138460130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring protein lipidation by mass spectrometry-based proteomics. 基于质谱的蛋白质组学探索蛋白质脂化。

IF 2.7 4区生物学

Journal of biochemistry Pub Date : 2024-03-04 DOI: 10.1093/jb/mvad109

Kazuya Tsumagari, Yosuke Isobe, Koshi Imami, Makoto Arita

引用次数: 0