Journal of biochemistry最新文献

Maintenance of the Golgi Ribbon Structure by the KASH Protein Jaw1. KASH 蛋白 Jaw1 维护高尔基体带状结构

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-18 DOI: 10.1093/jb/mvae081

Morihisa Fujita

引用次数: 0

Cellular senescence: mechanisms and relevance to cancer and aging. 细胞衰老：机制及其与癌症和衰老的关系。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-16 DOI: 10.1093/jb/mvae079

Shota Yamauchi, Akiko Takahashi

引用次数: 0

Bcl2l12, a novel protein interacting with Arf6, triggers Schwann cell differentiation program. Bcl2l12是一种与Arf6相互作用的新型蛋白质，可触发许旺细胞分化程序。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-07 DOI: 10.1093/jb/mvae078

Wakana Miyata, Naoko Sakaibara, Kentaro Yoshinaga, Asahi Honjo, Mikito Takahashi, Tatsuya Ooki, Hideji Yako, Kazunori Sango, Yuki Miyamoto, Junji Yamauchi

{"title":"Bcl2l12, a novel protein interacting with Arf6, triggers Schwann cell differentiation program.","authors":"Wakana Miyata, Naoko Sakaibara, Kentaro Yoshinaga, Asahi Honjo, Mikito Takahashi, Tatsuya Ooki, Hideji Yako, Kazunori Sango, Yuki Miyamoto, Junji Yamauchi","doi":"10.1093/jb/mvae078","DOIUrl":"https://doi.org/10.1093/jb/mvae078","url":null,"abstract":"Schwann cells are glial cells in the peripheral nervous system (PNS); they wrap neuronal axons with their differentiated plasma membranes called myelin sheaths. Although the physiological functions, such as generating saltatory conduction, have been well studied in the PNS, the molecular mechanisms by which Schwann cells undergo their differentiation program without apparent morphological changes before dynamic myelin sheath formation remain unclear. Here, for the first time, we report that Arf6, a small GTP/GDP-binding protein controlling morphological differentiation, and the guanine-nucleotide exchange factors cytohesin proteins are involved in the regulation of Schwann cell differentiation marker expression in primary Schwann cells. Specific inhibition of Arf6 and cytohesins by NAV-2729 and SecinH3, respectively, decreased expression of marker proteins 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and glial fibrillary acidic protein (GFAP). Similar results using promoter assays were observed using the IMS32 Schwann cell line. Furthermore, using an affinity-precipitation technique, we identified Bcl2-like 12 (Bcl2l12) as a novel GTP-bound Arf6-interacting protein. Knockdown of Bcl2l12 using a specific artificial miRNA decreased expression of marker proteins. The knockdown also led to decreased filamentous actin extents. These results suggest that Arf6 and Bcl2l12 can trigger Schwann cell differentiation, providing evidence for a molecular relay that underlies how Schwann cells differentiate.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The Hox-based positional memory in muscle stem cells. 更正：肌肉干细胞中基于 Hox 的位置记忆。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae066

引用次数: 0

Ciliary length variations impact cilia-mediated signaling and biological responses. 纤毛长度的变化会影响纤毛介导的信号传递和生物反应。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae057

Yuki Kobayashi, Akie Hamamoto, Yumiko Saito

{"title":"Ciliary length variations impact cilia-mediated signaling and biological responses.","authors":"Yuki Kobayashi, Akie Hamamoto, Yumiko Saito","doi":"10.1093/jb/mvae057","DOIUrl":"10.1093/jb/mvae057","url":null,"abstract":"Primary cilia are thin hair-like organelles that protrude from the surface of most mammalian cells. They act as specialized cell antennas that can vary widely in response to specific stimuli. However, the effect of changes in cilia length on cellular signaling and behavior remains unclear. Therefore, we aimed to characterize the elongated primary cilia induced by different chemical agents, lithium chloride (LiCl), cobalt chloride (CoCl2) and rotenone, using human retinal pigmented epithelial 1 (hRPE1) cells expressing ciliary G protein-coupled receptor (GPCR), melanin-concentrating hormone (MCH) receptor 1 (MCHR1). MCH induces cilia shortening mainly via MCHR1-mediated Akt phosphorylation. Therefore, we verified the proper functioning of the MCH-MCHR1 axis in elongated cilia. Although MCH shortened cilia that were elongated by LiCl and rotenone, it did not shorten CoCl2-induced elongated cilia, which exhibited lesser Akt phosphorylation. Furthermore, serum readdition was found to delay cilia shortening in CoCl2-induced elongated cilia. In contrast, rotenone-induced elongated cilia rapidly shortened via a chopping mechanism at the tip of the cilia. Conclusively, we found that each chemical exerted different effects on ciliary GPCR signaling and serum-mediated ciliary structure dynamics in cells with elongated cilia. These results provide a basis for understanding the functional consequences of changes in ciliary length.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"369-383"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of APBB1 as a substrate for anaplastic lymphoma kinase. 鉴定 APPB1 作为无性淋巴瘤激酶的底物。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae055

Yuji Suzuki, Shoma Tsubota, Kenji Kadomatsu, Kazuma Sakamoto

{"title":"Identification of APBB1 as a substrate for anaplastic lymphoma kinase.","authors":"Yuji Suzuki, Shoma Tsubota, Kenji Kadomatsu, Kazuma Sakamoto","doi":"10.1093/jb/mvae055","DOIUrl":"10.1093/jb/mvae055","url":null,"abstract":"Anaplastic lymphoma kinase (ALK) is a well-known oncogene involved in various malignancies such as anaplastic large cell lymphoma, lung cancer and neuroblastoma. Several substrates for fused ALK have been identified and their biological functions have been described. However, the lack of a comprehensive identification of ALK substrates limits our understanding of the biological roles of receptor ALK. Thus, this study aimed to identify novel ALK substrates and characterize their biological functions. We screened the interactors of the kinase domain of receptor ALK using proximity-dependent biotin identification and identified 43 interactors. We narrowed down the candidates by evaluating whether these interactors were downstream of ALK in a neuroblastoma cell line, NB-1. Amongst these, we identified amyloid beta precursor protein-binding family B member 1 (APBB1) as an ALK downstream molecule involved in NB-1 cell viability. Finally, we assessed the kinase-substrate relationship between ALK and APBB1 and found that ALK phosphorylated multiple tyrosine residues in APBB1 both in-cell and in-tube assays, with tyrosine 269 as a major target. In conclusion, we successfully identified a new substrate for receptor ALK. Our results may help further elucidate the molecular mechanism of ALK downstream signalling in neuroblastoma.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"395-403"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterizations of the central fragment of human Reelin and identification of amino acid residues involved in its secretion. 人类瑞林中心片段的生化特征以及参与其分泌的氨基酸残基的鉴定。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae058

Takao Kohno, Ikuma Nakagawa, Airi Taniguchi, Fang Heng, Mitsuharu Hattori

引用次数: 0

Dietary methionine functions in proliferative zone maintenance and egg production via sams-1 in Caenorhabditis elegans. 膳食蛋氨酸通过 sams-1 在秀丽隐杆线虫的增殖区维持和产卵过程中发挥作用。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae054

Keiko Hirota, Rieko Yamauchi, Mai Miyata, Mariko Kojima, Koichiro Kako, Akiyoshi Fukamizu

{"title":"Dietary methionine functions in proliferative zone maintenance and egg production via sams-1 in Caenorhabditis elegans.","authors":"Keiko Hirota, Rieko Yamauchi, Mai Miyata, Mariko Kojima, Koichiro Kako, Akiyoshi Fukamizu","doi":"10.1093/jb/mvae054","DOIUrl":"10.1093/jb/mvae054","url":null,"abstract":"The maintenance of germ cells is critical for the prosperity of offspring. The amount of food consumption is known to be closely related to reproduction, i.e. the number of eggs decreases under calorie-restricted conditions in various organisms. Previous studies in Caenorhabditis elegans have reported that calorie restriction reduces the number of eggs and the reduction can be rescued by methionine. However, the effect of methionine on the reproductive process has not been fully understood. In this study, to assess the gonadal function of methionine metabolism, we firstly demonstrated that a depletion in dietary methionine resulted in reduced levels of S-adenosyl-l-methionine (SAM) and S-adenosyl homocysteine in wild-type N2, but not in glp-1 mutants, which possess only a few germ cells. Second, we found no recovery in egg numbers upon methionine administration in SAM synthase (sams)-1 mutants. Furthermore, a reduced number of proliferative zone nuclei exhibited in the sams-1 mutants was not rescued via methionine. Thus, our results have shown that dietary methionine is required for the normal establishment of both the germline progenitor pool and fecundity, mediated by sams-1.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"359-367"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations associated with neurodevelopmental disorders affect ARF1 function and cortical development. 与神经发育障碍有关的变异会影响 ARF1 的功能和大脑皮层的发育。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae053

Tomoki Ishiguro, Mariko Noda, Masashi Nishikawa, Koh-Ichi Nagata, Hidenori Ito

{"title":"Variations associated with neurodevelopmental disorders affect ARF1 function and cortical development.","authors":"Tomoki Ishiguro, Mariko Noda, Masashi Nishikawa, Koh-Ichi Nagata, Hidenori Ito","doi":"10.1093/jb/mvae053","DOIUrl":"10.1093/jb/mvae053","url":null,"abstract":"ADP-ribosylation factors (ARFs) are a family of small GTPases that regulate vesicle trafficking and actin dynamics in cells. Recent genetic analyses have revealed associations between variations in ARF genes and neurodevelopmental disorders, although their pathophysiological significance remains unclear. In this study, we conducted biochemical, cell biological and in vivo analyses of ARF1 variants linked to neurodevelopmental disorders. The mant-GDP dissociation assay revealed that ARF1-p.R19C, -p.F51L, -p.R99C and -p.R99H exhibit higher GDP/GTP exchange activity compared to ARF1 wild type (WT). The GTPase-activating protein (GAP) increased the GTPase activity of WT, p.R19C, p.Y35H, p.F51L, p.P131L and p.P131R, but not of p.Y35D, p.T48I, p.R99C and p.R99H. The transient expression of p.R99C, p.R99H and p.K127E in mammalian cells resulted in the disruption of the Golgi apparatus. In utero electroporation-mediated gene transfer into the cortical neurons of embryonic mice demonstrated that p.R99C, p.R99H and p.K127E cause a migration defect. Expression of these variants resulted in the expansion of the Golgi apparatus in migrating cortical neurons. These findings suggest that the ARF1 variants linked to neurodevelopmental disorders, specifically p.R99C, p.R99H and p.K127E, disrupt the structure of the Golgi apparatus, thereby leading to a developmental defect of cortical neurons.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"347-357"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen Isolated from Human Adipose Tissue and Its Cellular Affinity. 从人体脂肪组织中分离出的胶原蛋白及其细胞亲和力

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-10-28 DOI: 10.1093/jb/mvae073

Hisayo Yamaoka, Keiko Yamaoka, Hidenori Ishii, Hideyuki Tanaka, Makoto Yasuda, Shigekazu Watanabe, Makoto Hosoyamada, Yuzo Komuro

{"title":"Collagen Isolated from Human Adipose Tissue and Its Cellular Affinity.","authors":"Hisayo Yamaoka, Keiko Yamaoka, Hidenori Ishii, Hideyuki Tanaka, Makoto Yasuda, Shigekazu Watanabe, Makoto Hosoyamada, Yuzo Komuro","doi":"10.1093/jb/mvae073","DOIUrl":"https://doi.org/10.1093/jb/mvae073","url":null,"abstract":"The use of collagen in cell cultures promotes cell proliferation and differentiation, and it has been commercialized. In this study, we separated and purified collagen from adipose tissue discarded during liposuction and prepared collagen-coated dishes. After collagen was identified from human adipose tissue, type identification and quantification were performed using SDS-PAGE and FPLC. Collagen type I was used to coat culture dishes. Human skin fibroblasts and human adipose tissue-derived stem cells were seeded at a density of 2.5×105 cells/mL on prepared dishes at a collagen concentration of 3 mg/mL and cultured for 7 days. Cell viability was then measured and analyzed. The WST-1 assay was used to evaluate the results. The amount of collagen in 300 g of adipose tissue was 25.5 mg for type I, 41.4 mg for type III, 10.6 mg for type IV, 6.5 mg for type V, and 15 mg for type VI. The highest rates were observed for adipose stem cells cultured on human adipose tissue-derived collagen-coated dishes. In cell cultures, cell affinity was higher when cells and the substrate used were of the same origin, and affinity was stronger when the tissue of origin was the same.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0