Journal of biochemistry最新文献

Scaffold-mediated phosphatase regulation in EGFR family signaling. 支架介导的磷酸酶调控EGFR家族信号。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-10-19 DOI: 10.1093/jb/mvag075

Shigeki Higashiyama, Eilma Akter-Yasuoka, Hiroyuki Takeda, Jun Nakayama

{"title":"Scaffold-mediated phosphatase regulation in EGFR family signaling.","authors":"Shigeki Higashiyama, Eilma Akter-Yasuoka, Hiroyuki Takeda, Jun Nakayama","doi":"10.1093/jb/mvag075","DOIUrl":"https://doi.org/10.1093/jb/mvag075","url":null,"abstract":"The epidermal growth factor receptor (EGFR) family serves as an archetypal model of receptor tyrosine kinase signaling and plays essential roles in development, tissue homeostasis, and cancer. Although receptor phosphorylation has been extensively studied, the mechanisms governing dephosphorylation remain comparatively obscured. A limited repertoire of protein tyrosine phosphatases (PTPs) directly regulates EGFR family receptors, yet their signaling specificity cannot be explained by intrinsic catalytic activity alone. Accumulating evidence indicates that PTP function is governed by higher-order mechanisms that control subcellular localization, receptor accessibility, and integration into dynamic signaling networks. In this Review, we summarize the PTPs that directly regulate the EGFR family and examine how scaffold proteins, membrane organization, intracellular trafficking, and organelle-specific platforms coordinate receptor dephosphorylation. We propose a functional taxonomy comprising five categories: assembly, localization, membrane-organizing, organelle, and regulatory scaffolds. This framework provides a basis for understanding signaling specificity and suggests therapeutic strategies targeting the spatiotemporal organization of phosphatase function.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering of pH-regenerable anti-insulin single chain variable fragments (scFvs) for continuous insulin monitoring. 用于胰岛素连续监测的ph可再生抗胰岛素单链可变片段（scFvs）工程。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-10-12 DOI: 10.1093/jb/mvag071

Qianming Xu, Ellie D Wilson, Mai Hamasaki, Hirobumi Suzuki, Ayumi Tanaka, Junko Okuda-Shimazaki, Miho Oda, Daimei Miura, Koji Sode, Ryutaro Asano

{"title":"Engineering of pH-regenerable anti-insulin single chain variable fragments (scFvs) for continuous insulin monitoring.","authors":"Qianming Xu, Ellie D Wilson, Mai Hamasaki, Hirobumi Suzuki, Ayumi Tanaka, Junko Okuda-Shimazaki, Miho Oda, Daimei Miura, Koji Sode, Ryutaro Asano","doi":"10.1093/jb/mvag071","DOIUrl":"https://doi.org/10.1093/jb/mvag071","url":null,"abstract":"To realize the continuous monitoring of insulin, the development of a regenerable engineered antibody is expected, whose binding constant is modulated by the mild in situ stimulation for future in vivo application. In this study, we aimed to develop pH-responsive anti-insulin antibodies based on a clinically established analytical antibody, the HUI-018 anti-insulin antibody. Its high-resolution co-crystal structure with human insulin (PDB ID: 6Z7W) provides a uniquely precise blueprint for molecular engineering by preparing engineered HUI-018 single-chain variable fragment (scFv). Among the engineered variants, the N93H/E101K mutant exhibited the biggest pH-responsiveness, showing a 5.2-folds increased dissociation rate at pH 6.0 while maintaining binding affinity and specificity comparable to wild-type (WT) at pH 7.4. Repeated cycles of insulin binding at pH 7.4 and regeneration at pH 6.0 demonstrated that the N93H/E101K mutant achieved substantially higher regeneration and rebinding efficiencies than WT. These results demonstrate that engineered pH-responsive antibody enables efficient binding site regeneration through mild pH change. To our knowledge, this is the first report of an anti-insulin antibody capable of binding site regeneration at pH 6.0, representing an important step toward antibody-based continuous insulin monitoring and continuous sensing of protein biomarkers.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149166435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NGS-Guided Discovery of Cross-Reactive TfR1 VHH Antibodies with Distinct Brain Localization Profiles. ngs引导下发现具有不同脑定位谱的交叉反应性TfR1 VHH抗体。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-10-10 DOI: 10.1093/jb/mvag070

Muhammad Feisal Jatnika, Asaki Nagashima, Wakana Miyahara, Abdur Rafique, Yuji Ito

{"title":"NGS-Guided Discovery of Cross-Reactive TfR1 VHH Antibodies with Distinct Brain Localization Profiles.","authors":"Muhammad Feisal Jatnika, Asaki Nagashima, Wakana Miyahara, Abdur Rafique, Yuji Ito","doi":"10.1093/jb/mvag070","DOIUrl":"https://doi.org/10.1093/jb/mvag070","url":null,"abstract":"Antibody delivery across the blood-brain barrier (BBB) remains challenging. We combined phage display with next-generation sequencing (NGS)-guided clone sequence-diverse selection to identify transferrin receptor 1 (TfR1)-binding VHH antibodies. Following alpacas immunization with human and rat TfR1, representative clones from distinct phylogenetic groups were screened for human/mouse cross-reactivity. Three clones (A1, Y11, and Z1) were reformatted as human Fc-mutant fusion proteins and characterized by ELISA, flow cytometry, and fluorescence imaging. All three clones recognized human and mouse TfR1, with ELISA EC₅₀ >100 nM. After intravenous administration in mice, clone Y11 showed sustained brain-associated fluorescence up to 48 h, whereas A1 showed an initially high signal that progressively declined. Fluorescent microscopy revealed preferential localization of Y11 to NeuN-positive neuronal regions, whereas A1 to lectin-positive vascular structures, indicating distinct brain localization profiles among the isolated clones. These findings identify cross-reactive TfR1-targeting VHHs with distinct brain-localization profiles and potential utility as platforms for compartment-selective CNS drug delivery.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149147277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MIF Family Cytokines as Common Mediators of UV-Induced Photocarcinogenesis and Photoaging. MIF家族细胞因子在紫外线诱导的光致癌和光老化中的作用。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-10-07 DOI: 10.1093/jb/mvag069

Tadamichi Shimizu

引用次数: 0

Microenvironmental Extracellular Matrix Remodeling Driven by GATA2 and GATA3 Deficiency Impairs Lymphatic Recanalization. 由GATA2和GATA3缺乏驱动的微环境细胞外基质重塑损害淋巴再通。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-09-30 DOI: 10.1093/jb/mvag068

Tomomi Watanabe-Asaka, Moyuru Hayashi, Takuya Harada-Norikane, Mayu Nakamura, May Kouriki, Jun Takai, Satoshi Uemura, Takuo Hirose, Yoshiko Kawai

{"title":"Microenvironmental Extracellular Matrix Remodeling Driven by GATA2 and GATA3 Deficiency Impairs Lymphatic Recanalization.","authors":"Tomomi Watanabe-Asaka, Moyuru Hayashi, Takuya Harada-Norikane, Mayu Nakamura, May Kouriki, Jun Takai, Satoshi Uemura, Takuo Hirose, Yoshiko Kawai","doi":"10.1093/jb/mvag068","DOIUrl":"https://doi.org/10.1093/jb/mvag068","url":null,"abstract":"GATA2 is a transcription factor that is essential for vascular and lymphatic development. GATA2 deficiency causes lymphatic malformations and lymphedema. Delayed lymphatic recanalization after popliteal lymph node removal in Gata2 heterozygous (Gata2+/-) mice is associated with collagen accumulation in the surrounding tissue. In this study, we investigated whether extracellular matrix (ECM) alterations contribute to lymphatic recanalization by analyzing subcutaneous tissue from Gata2+/- and Gata2 and Gata3 double heterozygous (Gata2+/-::Gata3+/-) mice. RNA-seq analysis revealed changes in ECM-related and adhesion-associated genes in both genotypes. Histological analyses demonstrated collagen accumulation and disorganized collagen bundles in Gata2+/-::Gata3+/- mice. Immunostaining further revealed reduced elastin fibers and decreased numbers of fibroblasts in subcutaneous tissues of Gata2+/- and Gata2+/-::Gata3+/- mice. These findings suggest that abnormalities in ECM organization, including altered collagen and elastin structures and reduced fibroblast populations, may underlie collagen accumulation and impaired lymphatic recanalization. Notably, Gata2+/-::Gata3+/- mice exhibited a patchwork-like dermal phenotype reflecting features of both single mutants, possibly owing to mosaic transcriptional regulation within the GATA gene network. The microenvironmental ECM structure plays an important role in lymphatic recanalization following lymphatic injury.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149080928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intrinsic endonucleolytic activity of snake venom phosphodiesterase. 蛇毒磷酸二酯酶的内在溶核活性。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-09-29 DOI: 10.1093/jb/mvag067

Narumi Aoki-Shioi, Yoshifumi Zaitsu, Ryosuke Shikasho, I-Jin Lin, Wen-Guey Wu, Isao Kuraoka

{"title":"Intrinsic endonucleolytic activity of snake venom phosphodiesterase.","authors":"Narumi Aoki-Shioi, Yoshifumi Zaitsu, Ryosuke Shikasho, I-Jin Lin, Wen-Guey Wu, Isao Kuraoka","doi":"10.1093/jb/mvag067","DOIUrl":"https://doi.org/10.1093/jb/mvag067","url":null,"abstract":"Snake venoms contain enzymes whose biochemical activities cannot be inferred reliably from sequence or proteomic detection alone. Snake venom phosphodiesterase (svPDE), an extracellular ENPP-family glycoprotein, is generally regarded as a 3'-to-5' exonuclease, although endonucleolytic activity was reported in early preparations. Whether this activity is intrinsic to a molecularly defined svPDE and how DNA structure influences cleavage remained unresolved. Here, nuclease activity in Protobothrops flavoviridis venom was tracked by sequential chromatography, and LC-MS/MS identified svPDE in active fractions. Comparable activity was detected in a commercial Crotalus atrox PDE I preparation. Purified, sequence- and structure-defined Naja atra svPDE converted covalently closed circular plasmid DNA to open-circular and then linear forms in a concentration-dependent manner; higher concentrations caused extensive degradation. Moreover, N. atra svPDE converted a plasmid containing a single nick at a defined position into linear DNA, and restriction mapping was consistent with complementary-strand cleavage at or near the nick. These results establish endonucleolytic activity as an intrinsic property of svPDE and suggest that its nuclease action may be influenced by structural features associated with DNA topology and strand discontinuities. Thus, svPDE may facilitate double-strand breakage at or near sites of single-strand discontinuity, expanding the biochemical repertoire of this conserved venom enzyme.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149080856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Replication protein A prevents unregulated priming and Rad51 loading on single-stranded DNA in nuclear extracts of Xenopus eggs. 复制蛋白A可防止爪蟾卵核提取物单链DNA上的无调节引物和Rad51负载。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-09-25 DOI: 10.1093/jb/mvag066

Taisei Miyata, Naoki Tani, Yoshitaka Kawasoe, Kei-Ichiro Ishiguro, Tatsuro S Takahashi

{"title":"Replication protein A prevents unregulated priming and Rad51 loading on single-stranded DNA in nuclear extracts of Xenopus eggs.","authors":"Taisei Miyata, Naoki Tani, Yoshitaka Kawasoe, Kei-Ichiro Ishiguro, Tatsuro S Takahashi","doi":"10.1093/jb/mvag066","DOIUrl":"https://doi.org/10.1093/jb/mvag066","url":null,"abstract":"In eukaryotes, single-stranded DNA (ssDNA) generated during DNA replication, recombination, and repair is rapidly bound and protected by the major single-stranded DNA-binding protein replication protein A (RPA). RPA not only stabilizes ssDNA but also acts as a central platform that coordinates diverse DNA transactions. Exhaustion of RPA due to unregulated ssDNA production leads to replication fork breakage and replication catastrophe, underscoring its critical role in genome stability. However, the direct consequences of RPA limitation remain incompletely understood. Using Xenopus egg extracts, we show that excess ssDNA induces spontaneous priming, a reaction that is otherwise prevented in a physiological nuclear environment. We provide evidence that priming suppression is mediated by stoichiometric binding of RPA to ssDNA. Analysis of the ssDNA-binding proteome reveals that RPA promotes the association of ATR checkpoint factors, Polα-primase, and the RFWD3 ubiquitin ligase with ssDNA. In contrast, RPA depletion induces the recruitment of Rad51, Rad51 paralogs, and Fbh1, a DNA helicase that interacts with both RPA and Rad51 and promotes fork breakage under replication stress. Collectively, our findings suggest that RPA contributes to genome stability by protecting ssDNA from inappropriate DNA synthesis and unscheduled recruitment of recombination and fork-processing factors.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149038857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target-Site Selection by Transcription Factors: Roles of DNA, Chromatin, and Cofactor-Mediated Regulation. 转录因子的靶位点选择：DNA、染色质和辅因子介导的调控的作用。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-09-16 DOI: 10.1093/jb/mvag065

Mitsuru Okuwaki

{"title":"Target-Site Selection by Transcription Factors: Roles of DNA, Chromatin, and Cofactor-Mediated Regulation.","authors":"Mitsuru Okuwaki","doi":"10.1093/jb/mvag065","DOIUrl":"https://doi.org/10.1093/jb/mvag065","url":null,"abstract":"Transcription factors (TFs) are sequence-specific DNA-binding proteins that regulate gene-expression programs and cell fate. The ability of a defined combination of four TFs to reprogram differentiated cells into induced pluripotent stem cells illustrates the powerful role of TFs in determining cellular identity. However, TFs usually recognize short and degenerate DNA motifs of approximately 6-12 base pairs, generating thousands to millions of potential motif matches in mammalian genomes. In living cells, TFs occupy only a restricted subset of these sites, indicating that motif presence alone is insufficient for functional target selection. Several layers of regulation contribute to this selective occupancy, including DNA methylation, nucleosome organization, histone modifications, chromatin remodeling, TF oligomerization, TF availability and localization, and cofactors that regulate DNA-binding domains. This review outlines how DNA/chromatin features and TF-centered mechanisms contribute to target-site selection. The principal aim is to highlight DNA-binding domain-directed cofactor regulation as an underappreciated mechanism that modulates TF-DNA binding and may help explain selective genomic occupancy.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148976802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterization of a homocysteine S-methyltransferase from Leuconostoc suionicum. 一种同型半胱氨酸s -甲基转移酶的生化特性研究。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-09-10 DOI: 10.1093/jb/mvag064

Tadao Oikawa, Nanami Harada, Sumire Hanafusa, Kazuya Yamanaka, Shiro Kato

{"title":"Biochemical characterization of a homocysteine S-methyltransferase from Leuconostoc suionicum.","authors":"Tadao Oikawa, Nanami Harada, Sumire Hanafusa, Kazuya Yamanaka, Shiro Kato","doi":"10.1093/jb/mvag064","DOIUrl":"https://doi.org/10.1093/jb/mvag064","url":null,"abstract":"We identified and characterized a homocysteine S-methyltransferase (HMT) from the lactic acid bacterium Leuconostoc suionicum LT-38, representing the first characterization of an HMT from lactic acid bacteria. The enzyme exhibited a kcat/Km value of 15.7 mM-1 s-1 toward L-homocysteine. It showed optimal activity at 30 °C and pH 7.5 and remained stable over a broad range of temperatures (30-60 °C) and pH values (5-9). The enzyme accepted both L- and D-homocysteine as methyl acceptors but displayed markedly higher catalytic efficiency toward the L-form (~30-fold). In contrast, L- and D-cysteine were not utilized. Both S-methyl-L-methionine and S-methyl-D-methionine were consumed in reactions containing racemic S-methyl-DL-methionine, and S-adenosyl-L-methionine was also accepted, whereas betaine and related compounds were not utilized. Gel filtration analysis indicated a monomeric structure (~32 kDa). Phylogenetic analysis showed that Ls-HMT clustered with bacterial HMT homologs and was distinct from mammalian BHMT/BHMT2 proteins. Inductively coupled plasma-mass spectrometry revealed approximately one Zn atom per enzyme molecule, and the Zn-depleted apo enzyme showed no detectable activity. These findings support that Ls-HMT is a Zn-dependent HMT with relatively high activity and expand the functional diversity of microbial HMTs.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical regulation of Ca2+ dynamics during muscle stem cell activation. 肌肉干细胞活化过程中Ca2+动力学的生物力学调控。

IF 2.4 4区生物学

Journal of biochemistry Pub Date : 2026-09-09 DOI: 10.1093/jb/mvag062

Kotaro Hirano, Yudai Ishikawa, Norio Motohashi, Yujiro Kobata, Hiroto Watanabe, Mao Sasaki, Takuya Yokoyama, Yuto Yamada, Yuta Aoyagi, Kanako Takakura, Akira Murakami, Masaki Tsuchiya, Yusuke Ono, Keiko Nonomura, Yoshitsugu Aoki, Yuji Hara

{"title":"Biomechanical regulation of Ca2+ dynamics during muscle stem cell activation.","authors":"Kotaro Hirano, Yudai Ishikawa, Norio Motohashi, Yujiro Kobata, Hiroto Watanabe, Mao Sasaki, Takuya Yokoyama, Yuto Yamada, Yuta Aoyagi, Kanako Takakura, Akira Murakami, Masaki Tsuchiya, Yusuke Ono, Keiko Nonomura, Yoshitsugu Aoki, Yuji Hara","doi":"10.1093/jb/mvag062","DOIUrl":"https://doi.org/10.1093/jb/mvag062","url":null,"abstract":"Muscle satellite cells (MuSCs) are muscle-resident stem cells that are responsible for myofiber regeneration. Although the importance of calcium ions (Ca 2+) in muscle physiology has been well established, the mechanism by which Ca 2+ mobilization governs MuSC function remains poorly understood. In this study, we aimed to systematically characterize Ca2+ dynamics in MuSCs and to define the mechanisms regulating these signals during muscle regeneration. By employing modified protocols for mouse MuSC isolation and Ca2+ measurement, we observed spontaneous Ca2+ fluctuations in MuSCs isolated from regenerating muscle after cardiotoxin-induced myofiber injury. Our detailed analysis using chemical Ca2+ indicators and a genetically encoded Ca2+ indicator revealed that the frequency and amplitude of Ca2+ fluctuations increased significantly during the activated and proliferative stages of MuSCs. This effect was more pronounced in MuSCs isolated from dystrophic and aged mice. Mechanistically, these Ca2+ fluctuations were at least partially mediated by mechanosensitive ion channels, including PIEZO1 and TRPM7, which promote MuSC migration. Collectively, our findings demonstrate that Ca2+ fluctuations through mechanosensitive ion channels act as a key regulator of MuSC activation during muscle regeneration and may provide new insights into the role of Ca2+ influx in muscle biology and the pathogenesis of muscle diseases.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148912202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0