成核是一种与凋亡相关的应激诱导蛋白,对脑神经炎症中的小胶质细胞极化/激活很重要。

IF 1.7 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Tuan Anh Pham, Takashi Sakai, Huy Van Dang, Diem Hong Tran, Yuji Shishido, Nam Hoang Tran, Kiyoshi Fukui
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引用次数: 0

摘要

小胶质细胞是中枢神经系统的巨噬细胞,对免疫防御至关重要,在感染期间保护神经元。它们在出生后大脑发育中的作用,特别是在受伤后,仍不清楚。成核蛋白是心肌分化过程中上调的一种蛋白,它调节NF-κB,影响细胞凋亡和细胞增殖。在这项研究中,我们使用野生型(WT)和核敲除(KO)新生小鼠进行聚(I:C)病毒模拟,研究了成核在小胶质细胞激活中的作用。Poly(I:C)处理增加了两个基因型的iba1阳性小胶质细胞;然而,KO小鼠在皮质和海马区均表现出明显夸张的反应。此外,虽然促炎M1标记物(iNOS、CD86、TNFα、IL-6)在WT和KO小鼠中均上调,但抗炎M2标记物精氨酸酶1 (Arg1)在WT中被诱导,而在KO小鼠中被显著抑制,表明M2极化受损。这些发现表明,成核对于维持小胶质细胞极化、支持免疫过程对抗病原体和帮助中枢神经系统发育至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nucling, a stress-inducible protein associated with apoptosomes, is important for microglial polarization/activation in the brain neuroinflamation.

Microglia, the central nervous system's resident macrophages, are critical for immune defense, protecting neurons during infection. Their role in postnatal brain development, particularly after injury, remains unclear. Nucling, a protein up-regulated during cardiac muscle differentiation, regulates NF-κB, influencing apoptosis and cell proliferation. In this study, we examined the role of Nucling in microglial activation using wild-type (WT) and Nucling-knockout (KO) neonatal mice subjected to poly(I:C), a viral mimic. Poly(I:C) treatment increased Iba1-positive microglia in both genotypes; however, KO mice showed a significantly exaggerated response in both cortical and hippocampal regions. Furthermore, while pro-inflammatory M1 markers (iNOS, CD86, TNFα, IL-6) were upregulated in both WT and KO mice, the anti-inflammatory M2 marker Arginase 1 (Arg1) was induced in WT but significantly suppressed in KO mice, indicating impaired M2 polarization. These findings suggest Nucling is essential for maintaining microglial polarization, supporting immunological processes against pathogens, and aiding central nervous system development.

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来源期刊
Journal of biochemistry
Journal of biochemistry 生物-生化与分子生物学
CiteScore
4.80
自引率
3.70%
发文量
101
审稿时长
4-8 weeks
期刊介绍: The Journal of Biochemistry founded in 1922 publishes the results of original research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written in English in the form of Regular Papers or Rapid Communications. A Rapid Communication is not a preliminary note, but it is, though brief, a complete and final publication. The materials described in Rapid Communications should not be included in a later paper. The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial Board.
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