Iranian Journal of Microbiology最新文献

{"title":"Anti-candida activity of <i>Lacticaseibacillus rhamnosus</i> R-2002 and its possible application in candidiasis prevention.","authors":"Aleksanyan Tigran, Hakobyan Lusine, Harutyunyan Ruzanna, Semerjyan Inesa, Nahapetyan Nelli, Gharibyan Edita, Haertlé Thomas, Bazukyan Inga","doi":"10.18502/ijm.v17i3.18827","DOIUrl":"10.18502/ijm.v17i3.18827","url":null,"abstract":"<p><strong>Background and objectives: </strong>Probiotics are used for the treatment of yeast infections, they restore the balance in vaginal microbiome, adhere to epithelial cells, compete against pathogenic bacteria, acidify the environment, produce bacteriocins and modulate the immunity. The aim of the study was to investigate the anti-yeast activity (AYA) of the strain <i>Lacticaseibacillus rhamnosus</i> R-2002 against different <i>Candida</i> species.</p><p><strong>Materials and methods: </strong>From 20 strains of lactic acid bacteria examined, only <i>L. rhamnosus</i> R-2002 strain demonstrated beneficial properties against yeast. The effects of temperature and pH on AYA and its relation to cell wall were revealed by bi-layer agar assay. The connection of AYA to the cell wall was determined with the sonicated cells.</p><p><strong>Results: </strong>R-2002 inhibited the growth of <i>C. albicans</i> ATCC 10291, <i>C. tropicalis</i> G 31 and <i>C. albicans</i> G4 (both isolated from vaginal samples). R-2002 maintained its AYA between a wide range of pH and its anti-yeast component/s are extracellular. The tested strain demonstrated stability against the high concentrations of progesterone and metronidazole, making it a suitable candidate for the mitigation of vaginitis.</p><p><strong>Conclusion: </strong>The present study summarizes all the positive features of the strain R-2002 and its potential as a therapeutic agent in the treatment of candidiasis.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"441-450"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the in vitro efficacy of antimicrobials against Enterobacterales with multiple carbapenemase enzymes.","authors":"Reena Rajan, Gopinathan Sasikala","doi":"10.18502/ijm.v17i3.18821","DOIUrl":"10.18502/ijm.v17i3.18821","url":null,"abstract":"<p><strong>Background and objectives: </strong>High-dose of carbapenems and combination therapies with new β-lactam/β-lactamase inhibitors and polymyxin B/tigecycline have been considered for treatment of carbapenem resistant Enterobacterales infection. The research was conducted to evaluate the in vitro potency of aminoglycosides, ceftazidime/avibactam/aztreonam and tigecycline against isolates of <i>Enterobacteriaceae</i> with multiple carbapenemase enzymes.</p><p><strong>Materials and methods: </strong>42 genotypically confirmed carbapenem resistant Enterobacterales (twenty-nine NDM producers, nine NDM and OXA-48 producers, three NDM and VIM producers and one NDM combined with VIM and OXA 48 producer) were included. Minimum inhibitory concentration for carbapenems, aminoglycosides and tigecycline was determined by Vitek 2. Ceftazidime/avibactam/aztreonam synergy was observed by disk diffusion methodology.</p><p><strong>Results: </strong>The in vitro efficacy of aminoglycosides was observed against <i>Escherichia coli (E. coli)</i> isolates with NDM and VIM genes. Low tigecycline susceptibility was observed among <i>Klebsiella pneumoniae (K. pneumoniae)</i> isolates with NDM and OXA-48 genes. Ceftazidime -avibactam/aztreonam combination displayed good in vitro activity against dual carbapenemase producers of <i>E. coli</i> isolates (NDM with OXA-48 and NDM with VIM genes) and <i>Klebsiella pneumoniae</i> (combination of NDM, VIM and OXA-48 genes).</p><p><strong>Conclusion: </strong>Ceftazidime/avibactam/aztreonam, aminoglycosides and tigecycline displayed in vitro activity against dual carbapenemase producers of <i>E. coli</i> and <i>K. pneumoniae.</i></p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"390-396"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy hearts: the impact of influenza on young lives, a rare case report.","authors":"Uksim Qadri, Sanam Wani, Saleem Javaid Wani, Bashir Ahmad Fomda, Umaya Majid, Iram Jan, Suraiya Mir","doi":"10.18502/ijm.v17i3.18834","DOIUrl":"10.18502/ijm.v17i3.18834","url":null,"abstract":"<p><p>Considerable information is available about the acute respiratory symptoms of influenza A and B. However, rarely, these viruses can adversely affect the cardiovascular system. Few cases of pericardial effusion and cardiac tamponade due to the Influenza virus have been reported. To the best of our knowledge, we present the first case of a 23-year-old unvaccinated woman having concurrent influenza A and B infection manifesting as pericardial effusion and cardiac tamponade. The patient was treated with oseltamivir 75 mg, resulting in significant clinical improvement. This case emphasizes the importance of considering influenza as a possible cause of cardiac tamponade.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"511-515"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic, genotypic characterization and antimicrobial resistance profiling of uropathogenic <i>Escherichia coli</i> in a tertiary care hospital, Puducherry, India.","authors":"Sherief Shebeena, Latha Ragunathan, Kavitha Kannaiyan, Thiyagarajan Sanjeevi, Pramodhini Subramanian, Aravind C Sasi, Jaishma Rajni, Jenifer Raj, Mohamed Asarudeen","doi":"10.18502/ijm.v17i3.18818","DOIUrl":"10.18502/ijm.v17i3.18818","url":null,"abstract":"<p><strong>Background and objectives: </strong>Uropathogenic <i>Escherichia coli</i> (<i>E. coli)</i> (UPEC) accounts for 70-95% of community-acquired urinary tract infections (UTIs) and a significant proportion of nosocomial UTIs. This study aimed to characterize the phenotypic and genotypic characteristics of <i>E. coli</i> isolates from symptomatic UTI patients and evaluate their antimicrobial susceptibility patterns.</p><p><strong>Materials and methods: </strong>A hospital-based observational study was conducted at Aarupadai Veedu Medical College and Hospital, Puducherry, India, from August 2022 to April 2024. A total of 106 UPEC isolates were obtained from symptomatic UTI patients. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer method, and virulence genes (<i>hlyA, fimH, papC</i>) were detected using PCR.</p><p><strong>Results: </strong>The mean age of patients was 49.7 years, with a female predominance (69.8%). Diabetes mellitus was the most common comorbidity (29.2%). Fever (60.4%) and dysuria (38.7%) were the most common symptoms. AST showed high susceptibility (>90%) to amikacin, nitrofurantoin, meropenem, and piperacillin/tazobactam, while >60% resistance was observed to cefotaxime and ceftazidime. Phenotypically, 30.2% of the isolates produced mannose-resistant hemagglutinins, and 17.9% produced hemolysin. ESBL production was found in 46.3%. Biofilm production was moderate in 65.1%, weak in 30.2% and strong in 4.7% and significantly correlated with multidrug resistance (p<0.05). Genotypically, 80.2% had <i>fimH</i>, 51.9% had <i>papC</i> and 20.8% had <i>hlyA. papC</i> was associated with reduced cefotaxime susceptibility (p<0.05).</p><p><strong>Conclusion: </strong>The study highlights the significance of phenotypic and genotypic characterization in understanding UPEC virulence and resistance patterns, and emphasizes the need for targeted empiric therapy to improve UTI management.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"366-375"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactivities of postbiotics in food applications: a review.","authors":"Saeedeh Jahedi, Safoora Pashangeh","doi":"10.18502/ijm.v17i3.18816","DOIUrl":"10.18502/ijm.v17i3.18816","url":null,"abstract":"<p><p>Postbiotics, which consist of beneficial compounds produced by probiotic bacteria, have emerged as promising natural preservatives in food applications. This article examines the health-promoting properties of postbiotics and their role in improving food preservation and formulating nutrient-enriched foods. An organized investigation of published works was carried out through key research databases, including ScienceDirect, Scopus, PubMed, and Google Scholar, using keywords such as \"Postbiotics,\" \"Biopreservation,\" \"Food Safety,\" \"Functional Foods,\" \"Antimicrobial Activity,\" \"Anti-inflammatory,\" and \"Bioactivities\". The findings reveal that postbiotics exert antimicrobial effects through multiple mechanisms, including the production of organic acids, bacteriocins, fatty acids, antimicrobial peptides, hydrogen peroxide, and vitamins. These bioactive substances actively suppress the proliferation of harmful and spoilage-causing microbes, consequently prolonging the preservation period of food items. Furthermore, postbiotics have been integrated into functional foods to modulate the host immune response and mitigate inflammatory conditions. Emerging applications of postbiotics also include their use in active food packaging systems, biofilm eradication, and cosmetic formulations. Although research on postbiotics is advancing, further investigations are required to elucidate the mechanisms of postbiotics and optimize their applications in both clinical and non-clinical contexts. This review emphasizes the potential of postbiotics to enhance food safety, improve nutritional quality, and contribute to overall health promotion.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"348-357"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological and molecular detection of biofilm formation and antibiotic resistance genes of <i>Pseudomonas aeruginosa</i> isolated from urinary tract.","authors":"Hatem Sahib Abed, Seyed Masoud Hosseini, Zainab Mohammed Jassim","doi":"10.18502/ijm.v17i3.18819","DOIUrl":"10.18502/ijm.v17i3.18819","url":null,"abstract":"<p><strong>Background and objectives: </strong><i>Pseudomonas aeruginosa (P. aeruginosa)</i> is one of the most common causes of hospital-acquired infections. It is associated with high morbidity and healthcare costs, especially when appropriate antibiotic treatment is delayed. Antibiotic selection for patients with <i>P. aeruginosa</i> infections is challenging due to the bacteria's inherent resistance to many commercially available antibiotics. This study investigated antibiotic-resistance genes in isolated bacteria, which play a key role in disease pathogenesis.</p><p><strong>Materials and methods: </strong>100 samples out of the 140 samples collected from urinary tract infections (UTIs) cases between December 15^th, 2022, and April 15^th, 2023, were included in the study. Identification of bacterial isolates was based on colony morphology, microscopic examination, biochemical tests, and the Vitek-2 system. Antibiotic resistance genes; <i>Aph(3)-llla, ParC, Tet/tet(M),</i> and <i>aac(6´)-Ib-cr</i> were tested by polymerase chain reaction (PCR).</p><p><strong>Results: </strong>The obtained results were based on bacterial identifications of 81 clinical samples. Only 26 (32%) of these isolates were <i>P. aeruginosa</i>, 21 (26%) were <i>Escherichia coli</i>, and 18 (22.2%) were other bacteria. These isolates were used to detect four genes including <i>tet(M), Aph(3)-llla, Par-c,</i> and <i>aac(6´)-Ib-cr.</i> Four types of primers were used for PCR detection. The results showed that 11/14 (78.57%) carried the <i>tet(M)</i> gene, 10/14 (71.42%) carried the <i>Aph(3)-llla</i> gene, 14/14 (100%) carried the <i>Par-c</i> gene, and 10/14 (71.42%) of the isolates carried the <i>aac(6´)-Ib-cr</i> gene. The biofilm formation examining the <i>esp</i> gene, showed that 9 (64.28) isolates carried this gene.</p><p><strong>Conclusion: </strong>The inability of antibiotics to penetrate biofilms is an important factor contributing to the antibiotic tolerance of bacterial biofilms.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"376-381"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and comparison of PCR-ELISA reaction with other available hybridization methods to identify types 11, 16, and 18 of the human papillomavirus.","authors":"Seyed Mohammad Amin Mousavi-Rad, Shohreh Zare Karizi, Hamid Sedighian, Seyed Ali Mirhosseini, Hadi Esmaeili Gouvarchin Ghaleh, Jafar Amani","doi":"10.18502/ijm.v17i3.18832","DOIUrl":"10.18502/ijm.v17i3.18832","url":null,"abstract":"<p><strong>Background and objectives: </strong>Human papillomavirus (HPV) is a significant etiological agent in cervical cancer. This study aimed to evaluate the performance of PCR-ELISA for detecting HPV genotypes 11, 16, and 18 compared to the conventional hybridization methods.</p><p><strong>Materials and methods: </strong>PCR-ELISA was designed and optimized to detect target HPV genotypes using biotin-labeled probes. Sensitivity, specificity and reproducibility were assessed through intra-assay and inter-assay variability tests. Additionally, a cost-benefit analysis was performed to compare PCR-ELISA with RT-PCR and gel electrophoresis.</p><p><strong>Results: </strong>PCR-ELISA demonstrated high sensitivity (HPV18: 94.92%, HPV16: 98.36%, HPV11: 93.75%) and specificity (100% for all genotypes), with Kappa values ranging from 0.84 to 0.92, indicating strong agreement with the reference standard. Reproducibility analysis showed intra-assay CVs below 5% for most samples and inter-assay CVs within acceptable limits. The cost-benefit analysis revealed significant reductions in reagent and equipment costs compared to RT-PCR, making PCR-ELISA a cost-effective alternative.</p><p><strong>Conclusion: </strong>PCR-ELISA offers a reliable, sensitive and cost-effective method for HPV detection, particularly in resource-limited settings. Its simplicity and compatibility with existing workflows makes it a promising tool for routine diagnostic applications.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"488-502"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the prevention, treatment and synergistic activity between <i>Helicobacter pylori</i> IgY antibodies and pantaprazole on AGS cell line and C57BL/6 Mice.","authors":"Sara Kamal Shahsavar, Zahra Esmaeili, Hamid Ariannejad, Mohammadreza Nassiri, Ali Beheshti Namdar, Amir Hossein Jafarian, Ramin Sadeghi, Vahid Reza Dabbagh, Kiarash Ghazvini","doi":"10.18502/ijm.v17i3.18823","DOIUrl":"10.18502/ijm.v17i3.18823","url":null,"abstract":"<p><strong>Background and objectives: </strong>The growing resistance of <i>Helicobacter pylori</i> to antibiotics poses significant challenges in managing gastric ulcers. The application of IgY antibodies against <i>H. pylori</i> is a promising strategy. The current study evaluated the preventive and synergistic effects of IgY antibodies in conjunction with pantoprazole for treating <i>H. pylori</i> infection.</p><p><strong>Materials and methods: </strong>This investigation specifically focused on the inhibitory effects of IgY in the AGS cell line infected with <i>H. pylori</i>. In addition, the synergistic activity of IgY with pantoprazole and its preventive and therapeutic effects were assessed in male C57BL/6 mice.</p><p><strong>Results: </strong>The findings indicated that IgY antibodies possess a substantial inhibitory effect on the adhesion of <i>H. pylori</i> to the AGS cell line. Furthermore, IgY antibodies resulted in a significant reduction (P<0.05) in the population of <i>H. pylori</i> and the severity of gastritis in infected C57BL/6 mice in both treatment and prevention groups. Notably, the optimal outcome was observed when IgY was administered alongside pantoprazole.</p><p><strong>Conclusion: </strong>The use of IgY has the potential to repair damaged cells and prevent infection by decreasing bacterial adherence to gastric epithelial cells. Given its synergistic effect with pantoprazole, IgY can be recommended as a suitable complementary treatment in conjunction with pantoprazole.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"404-410"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of <i>Helicobacter pylori</i>-derived outer membrane vesicles (OMVs) on Snail/β-Catenin cascade and metastasis-related proteins in 4T1 breast cancer cells.","authors":"Seyedeh Shadi Vaziri, Elahe Tajbakhsh, Faham Khamesipour, Hassan Momtaz","doi":"10.18502/ijm.v17i3.18824","DOIUrl":"10.18502/ijm.v17i3.18824","url":null,"abstract":"<p><strong>Background and objectives: </strong>This study investigates the impact of <i>Helicobacter pylori (H. pylori)</i>-derived outer membrane vesicles (OMVs) on the regulation of Snail/β-Catenin cascade and the production of metastasis-related proteins, such as E-cadherin and Vimentin, in the 4T1 cell line.</p><p><strong>Materials and methods: </strong>OMVs were purified from <i>H. pylori</i> (ATCC 700392) cultures and applied to 4T1 cells at concentrations of 1, 5, and 10 μg/mL, with untreated cells serving as controls. The MTT assay was employed to quantify cell viability. Expression profiles of +<i>vimentin, Snail, α-SMA</i>, and <i>β-catenin</i> genes were evaluated by qRT-PCR, while protein expression of E-cadherin and Vimentin was analyzed via immunohistochemistry. Data were analyzed using appropriate statistical methods with SPSS and GraphPad Prism software.</p><p><strong>Results: </strong>The MTT assay showed that 1 μg/mL OMVs were safe for normal cells. At this concentration, the expression of <i>vimentin, Snail, α-SMA</i>, and <i>β-catenin</i> genes significantly increased in the treatment group (P≤0.05). Additionally, Vimentin protein decreased, and E-cadherin protein increased (P≤0.05).</p><p><strong>Conclusion: </strong><i>H. pylori</i>-derived OMVs activate the Snail/β-Catenin cascade, influencing inflammatory responses and metastasis-related proteins, ultimately reducing migration in advanced cancer stages by modulating Vimentin and E-cadherin expression.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"411-419"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of lactic acid bacteria isolated from dairy products on lipid pattern of rats fed with a high fat diet.","authors":"Sahar Fouladi, Elham Moazamian","doi":"10.18502/ijm.v17i3.18826","DOIUrl":"10.18502/ijm.v17i3.18826","url":null,"abstract":"<p><strong>Background and objectives: </strong>A unique characteristic of probiotics is obesity and fatty liver control. In this study, the effect of lactic-acid-bacteria (LABs) isolated from dairy products was investigated on weight changes, blood biochemical indexes and liver tissue in mice fed a high-fat diet.</p><p><strong>Materials and methods: </strong>A total of 49 rats were assigned to 7 groups. The LAB-treated groups received the high-cholesterol diet supplemented with <i>Lactobacillus reuteri, Lactobacillus plantarum</i>, and <i>Bifidobacterium animalis</i> isolated from yogurt. At the end of 4 weeks, body weight changes, lipid factors and liver enzymes as well as liver lipid deposition and adipocyte size were measured.</p><p><strong>Results: </strong>Serum low-density lipoprotein, total cholesterol, triglyceride levels and hepatic lipid deposition were significantly decreased in rats treated with LABs. The maximum and minimum weights were observed in the first and fourth weeks after treating with <i>Lactobacillus</i> and <i>Bifidobacterium</i> isolates, respectively. Liver enzymes were significantly decreased by LABs, especially in the group receiving concomitant administration of <i>Lactobacillus</i> and <i>Bifidobacterium</i>. Fatty liver process was reduced in the fat-fed group treated with <i>L. reuteri.</i></p><p><strong>Conclusion: </strong>LABs caused decreases in body weight gain, liver function, and adipocyte size. Therefore, coadministration of <i>Lactobacillus</i> and <i>Bifidobacterium</i> in dairy products can significantly decrease lipid profile.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"434-440"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}