Iranian Journal of Microbiology最新文献_第3页

Prevalence, antimicrobial resistance, virulence gene distribution and SCCmec typing of methicillin-resistant Staphylococcus aureus isolated from raw milk and dairy products. 从生乳和乳制品中分离出的耐甲氧西林金黄色葡萄球菌的流行率、抗菌药耐药性、毒力基因分布和 SCCmec 分型。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16793

Najmeh Vahed Dehkordi, Ebrahim Rahimi, Noosha Zia Jahromi

{"title":"Prevalence, antimicrobial resistance, virulence gene distribution and SCCmec typing of methicillin-resistant Staphylococcus aureus isolated from raw milk and dairy products.","authors":"Najmeh Vahed Dehkordi, Ebrahim Rahimi, Noosha Zia Jahromi","doi":"10.18502/ijm.v16i5.16793","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16793","url":null,"abstract":"Background and objectives: Researchers have focused on Staphylococcus aureus because it is transmitted through food, such as milk and dairy products, and causes human diseases. Prevalence, antimicrobial resistance, presence, and distribution of methicillin-resistant S. aureus (MRSA) virulence genes isolated from raw milk and dairy products were evaluated.Materials and methods: 300 samples of dairy products were collected from Shahrekord, Iran. S. aureus was identified using biochemical tests and screened for sensitivity to 13 antibiotics to identify resistance genes. In addition, SCCmec typing was performed.Results: Out of 300, S. aureus was found in 82 samples. Raw milk had the highest contamination with S. aureus (60 of 82), followed by cheese (15 of 82), and butter (7 of 82). At least one resistance gene was present in every isolate of S. aureus. Virulence factors and enterotoxin-coding genes, such as sea, seb, sec, and sed were highly distributed.Conclusion: The results of this study revealed the presence of toxin-producing MRSA strains in raw milk and dairy products. MRSA in dairy farms is an important risk factor for the spread of staphylococcal infections; therefore, further studies are needed to find strategies for controlling the presence of S. aureus, especially MRSA, in dairy products.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"605-613"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is routine screening for Candida auris necessary in ICU? 重症监护病房是否有必要对念珠菌进行常规筛查？

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16807

Arun Sachu, Harisree Sudersanan, Sanjo Sunny, Philip Mathew, Ajeesh Kumar, Alice David

{"title":"Is routine screening for Candida auris necessary in ICU?","authors":"Arun Sachu, Harisree Sudersanan, Sanjo Sunny, Philip Mathew, Ajeesh Kumar, Alice David","doi":"10.18502/ijm.v16i5.16807","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16807","url":null,"abstract":"Background and objectives: The capability to cause invasive infection, multi-drug resistance, and health care-associated outbreaks of Candida auris have made it a pathogen of great concern. Estimating how many patients in our intensive care unit had C. auris colonization and what characteristics put patients at risk for having Candida spp. colonization were the primary goals of the study.Materials and methods: Swabs from axilla and groin were collected from 229 patients getting admitted to the ICU. Samples were inoculated into CHROMagarTM Candida Plus medium. Colonies presumptively identified as C. auris by the presence of light blue with blue halo and were confirmed by VITEK-2.Results: Our study showed that only one patient was colonized with C. auris. A total of 47 (20.5%) patients were colonized with Candida spp., of which Candida parapislosis was the predominant organism. History of antibiotic use and cerebrovascular accident were independent risk factors in Candida colonization.Conclusion: Active screening for Candida auris in all patients is not required in our hospital as the prevalence was very low and not cost-effective. Therefore we plan to modify our screening strategy and use risk factors based surveillance strategy as it may serve as an ideal strategy.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"708-715"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased incidence of methicillin resistant Staphylococcus aureus and methicillin resistant Staphylococcus epidermidis in the skin and nasal carriage among healthcare workers and inanimate hospital surfaces after the COVID-19 pandemic. COVID-19 大流行后，医护人员和医院无生命表面的皮肤和鼻腔携带物中耐甲氧西林金黄色葡萄球菌和耐甲氧西林表皮葡萄球菌的发病率增加。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16791

Esraa H Al-Nsour, Hadeel T Al-Hadithi, Rania Mhammad Al-Groom, Saqr Abushattal, Abdallah Y Naser, Ahmad H Al Nsour, Rawand A Sallam, Lara M Kollab, Laila Alswalha, Mohd Sajjad Ahmad Khan

{"title":"Increased incidence of methicillin resistant Staphylococcus aureus and methicillin resistant Staphylococcus epidermidis in the skin and nasal carriage among healthcare workers and inanimate hospital surfaces after the COVID-19 pandemic.","authors":"Esraa H Al-Nsour, Hadeel T Al-Hadithi, Rania Mhammad Al-Groom, Saqr Abushattal, Abdallah Y Naser, Ahmad H Al Nsour, Rawand A Sallam, Lara M Kollab, Laila Alswalha, Mohd Sajjad Ahmad Khan","doi":"10.18502/ijm.v16i5.16791","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16791","url":null,"abstract":"Background and objectives: Healthcare workers in hospitals are exposed to infectious diseases that occur in the hospital making them a source of infection for the patients. It is interfaced as cross-contamination agents for MRSA and MR-CoNS, and preventive measures need to be adapted accordingly. The study aimed to assess Methicillin-Resistant Staphylococcus (MRS) on the skin and nasal cavities of healthcare workers (HCWs) and identifying isolates to the species level.Materials and methods: Swab samples were cultured on mannitol salt agar (MSA) to obtain MRS and determine their ability to produce coagulase. Their susceptibility to antibiotics were determined by agar screening and disk diffusion methods and further identification was done at the species level.Results: The highest percentage of methicillin resistant coagulase positive Staphylococci (MRCoPS) was reported among skins of male HCWs, (71.4%) were identified as MRSA. The highest levels of methicillin resistant coagulase negative Staphylococci (MRCoNS) were mainly detected in both nasal cavities, (75%) were identified as MRSE. MRSA was reported from doctors (p-value 0.033), whereas the highest incidence of MRSE was obtained from the nurses (p-value 0.048).Conclusion: This study highlighted that incidence of MRSA was mainly detected in doctors and MRCoNS in both nasal cavities. The highest percentage of MRCoNS was recovered from the patients' room followed by the reception table. Moreover, vancomycin is suggested to be highly effective in managing and controlling S. aureus, MRSA- and MRSE related infections.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"584-597"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of Epstein-Barr virus in paraffin-embedded tissue samples of patients suffering gastric cancer in Ahvaz, Iran: a case-control study. 伊朗阿瓦士胃癌患者石蜡包埋组织样本中 Epstein-Barr 病毒的分子检测：一项病例对照研究。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16805

Mehdi Gharibzadeh, Shahram Jalilian, Manoochehr Makvandi, Seyed Saeid Seyedian, Azarakhsh Azaran

{"title":"Molecular detection of Epstein-Barr virus in paraffin-embedded tissue samples of patients suffering gastric cancer in Ahvaz, Iran: a case-control study.","authors":"Mehdi Gharibzadeh, Shahram Jalilian, Manoochehr Makvandi, Seyed Saeid Seyedian, Azarakhsh Azaran","doi":"10.18502/ijm.v16i5.16805","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16805","url":null,"abstract":"Background and objectives: Gastric cancer (GC) is the third most common cause of cancer-related mortality. Epstein-Barr virus (EBV) is associated with several human tumors. The present research was performed to investigate the prevalence of EBV-associated gastric cancer (EBVaGC) among Iranian patients.Materials and methods: Seventy cases of gastric cancer and 30 cases of gastric ulcer, all preserved in formalin-fixed paraffin-embedded (FFPE), were examined in a case-control study conducted between 2011 and 2018. The specimens underwent analysis to detect the presence of the EBV genome using a Nested-PCR method targeting EBNA1. Subsequently, samples testing positive for the EBNA1 underwent further testing for the presence of the EBER gene using PCR. Finally, Positive samples were subjected to sequencing.Results: Five out of 70 cases (7%) were found to be positive for EBV based on EBNA1 testing, while all EBNA1 positive samples were negative for EBER. Notably, EBV was not detected in patients with gastric ulcer. The mean age of EBV-positive gastric carcinomas pateints was 64.5 years. Within this group, 60% were male and 40% were female. A higher prevalence of EBV association was observed in diffuse-type cases, with 60% (3 out of 24) testing positive, compared to intestinal-type cases where 40% (2 out of 46) were EBV-positive. Most cases of EBVaGC belonged to grade Ⅰ.Conclusion: This research demonstrates a low prevalence of EBVaGC in Iran. Discrepancies in EBVaGC occurrence among countries could be attributed to epidemiological variables and dietary practices. A comprehensive studies will provide significant contributions to understanding of its etiology.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"689-697"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust contact tracing and screening needed for leprosy control and protection of vulnerable children. 控制麻风病和保护弱势儿童需要强有力的接触者追踪和筛查。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16808

Khyathi Reddy Pilaka, Michael Sukumar Pallapati, Zakaraiah Jaladi, Kumar Ebineshan

引用次数: 0

Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients. 造血干细胞移植患者的肠道微生物群与移植物抗宿主疾病。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16800

Pegah Panahi, Amir Hossein Hashemian, Mehrdad Payandeh, Mahdi Taghadosi, Bizhan Nomanpour

{"title":"Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients.","authors":"Pegah Panahi, Amir Hossein Hashemian, Mehrdad Payandeh, Mahdi Taghadosi, Bizhan Nomanpour","doi":"10.18502/ijm.v16i5.16800","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16800","url":null,"abstract":"Background and objectives: Graft-versus-host disease (GvHD) frequently complicates hematopoietic stem cell transplantation (HSCT). Emerging evidence suggests a correlation between gut microbiota and GvHD risk. This study aims to elucidate the microbiota profiles in HSCT patients before and after transplantation and their association with GvHD.Materials and methods: This study, conducted from December 2022 to December 2023, involved the collection of 15 stool samples from HSCT patients. Bacterial content was quantified using real-time PCR, while interleukin-6 levels were assessed via ELISA.Results: Among the 15 participants (8 male, 7 female), 9 underwent allogeneic HSCT (allo-HSCT) and 6 received autologous HSCT. In the aGvHD group, there was a significant reduction in the abundance of Bacteroides and Bifidobacterium compared to those without aGvHD. Additionally, declines were observed in Clostridium and Firmicutes populations. The genus Blautia also showed reduced prevalence in the aGvHD group, whereas no significant differences were noted in the uncomplicated group. ELISA analysis revealed that interleukin-6 levels remained within the normal range (30-960 pg/ml) with no significant elevation in the aGvHD group.Conclusion: The study highlights a notable association between alterations in gut microbiota, specifically reductions in certain bacterial populations and the development of aGvHD following allo-HSCT.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"648-654"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New nano-chemotherapeutic chitosans-garlic oil-antibiotics against diabetic foot virulent Proteus spp. 新型纳米化学治疗壳聚糖-大蒜油-抗生素对糖尿病足带毒变形杆菌的作用

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16802

Khouloud M Barakat, Yousry M Gohar, Hasnaa E-B Ghonam, Ghada A Bashir

{"title":"New nano-chemotherapeutic chitosans-garlic oil-antibiotics against diabetic foot virulent Proteus spp.","authors":"Khouloud M Barakat, Yousry M Gohar, Hasnaa E-B Ghonam, Ghada A Bashir","doi":"10.18502/ijm.v16i5.16802","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16802","url":null,"abstract":"Background and objectives: Diabetes foot ulcer is recognized to have a major side effect that raises the risk of amputation. Diabetic ulcer bacterial infections caused by virulent and resistant bacteria like Proteus mirabilis lead to serious wounds that are incurable with conventional medications.Materials and methods: In this study, we evaluated the antibacterial activity of a natural product nanochitosan - garlic oil against ten diabetic foot isolates of Proteus mirabilis. Various chitosans (Crab (CScr) - shrimp (CSsh) - squilla (CSsq)) in nano form were prepared and coated with garlic oil (GO). GC-MS analysis was carried out to determine the main components of the essential garlic oil. The physicochemical properties of GO-NCSsq were analyzed using dynamic light scattering (DLS), zeta potentials (ZP) and subsequently scan electron microscope (SEM). Additionally, the minimum inhibitory concentration (MIC) and fraction inhibitory concentration index (FICI) were determined.Results: GC-MS analysis revealed the presence of major palmitic fatty acid. (GO) loaded on nanochitosan squilla (NCSsq) showed high activity. Although SEM showed lower nano-size, average size of the GO-NCSsq was 330.8 nm by DLS and its zeta potential formulation was +39.6 mV. The final formulation represented by GO-NCSsq + Pipercillin (Pi) inhibited the virulence factor of P. mirabilis and reduced the MIC value (p-value > 0.001). Moreover, the killing time at 9 h was found to be significantly higher for GO-NCSsq + pipercillin (Pi) against P. mirabilis.Conclusion: In order to manage diabetic foot infections, GO-NCSsq is a legitimate antibacterial agent that can be coupled with other antibiotics.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"666-675"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of chitosan nanogels loaded with vancomycin and gamma interferon on TNF-α gene expression in macrophage cell line activated with methicillin-resistant Staphylococcus aureus (MRSA). 载入万古霉素和γ干扰素的壳聚糖纳米凝胶对耐甲氧西林金黄色葡萄球菌（MRSA）激活的巨噬细胞系中 TNF-α 基因表达的影响。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16794

Sahar Arkiya, Ardeshir Hesampour, Ali Esrafili, Javad Arasteh

{"title":"Effect of chitosan nanogels loaded with vancomycin and gamma interferon on TNF-α gene expression in macrophage cell line activated with methicillin-resistant Staphylococcus aureus (MRSA).","authors":"Sahar Arkiya, Ardeshir Hesampour, Ali Esrafili, Javad Arasteh","doi":"10.18502/ijm.v16i5.16794","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16794","url":null,"abstract":"Background and objectives: Staphylococcus aureus is an opportunistic pathogen that frequently leads to asymptomatic infections. Methicillin-resistant strains (MRSA) pose a significant threat as they are resistant to most commonly used antibiotics, complicating treatment efforts. This study aimed to develop chitosan nanogels loaded with vancomycin and IFN-γ and to assess the expression of the TNF-α gene in a cell line infected with MRSA.Materials and methods: Following the synthesis and confirmation of the chitosan nanogels, vancomycin and IFN-γ were incorporated into these nanogels. The synthesis was validated using DLS, FTIR, TEM, and SEM. Subsequently, the anti-bacterial efficacy of the nanogels was assessed. Finally, four groups of cell lines were designed: control, MRSA, chitosan nanogels and IFN-γ-vancomycin chitosan nanogels. After infection of the groups (except control) with MRSA, 5 μg/mL of nanogels, and nanogels (drug and IFN-γ) were added to groups 3 and 4, respectively. Then the expression of TNF-α gene in each group was analyzed by RT-PCR at 6 and 24 hours.Results: At pH 6.5 and 7.4, the MIC of 1 μg/mL was obtained for free vancomycin, whereas that of IFN-γ-vancomycin nanogels at both pHs was respectively 8 and 64 μg/mL. The IC50 of chitosan nanogels and nanogels loaded with vancomycin-IFN-γ on RAW264.7 cells were 2.37 and 4.15 μg/mL in 24 hours, respectively. In group 4 in comparison to the MRSA group, TNF-α expression decreased significantly following 24 hours.Conclusion: Loading of vancomycin and IFN-γ in the chitosan nanogel can reduce TNF-α gene expression on MRSA infected cell lines.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"614-623"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MIC trends of vancomycin and teicoplanin among methicillin resistant CoNS isolates from new born blood cultures in a tertiary care centre in Southern India. 印度南部一家三级医疗中心新生儿血液培养物中耐甲氧西林的 CoNS 分离物中万古霉素和替考拉宁的 MIC 变化趋势。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16792

Neethu John, Chinnu Sajeev, Greeshma Hareendranath, Tanya Tonny Mampilly, Aswathy Varghese, Lancy Justus

{"title":"MIC trends of vancomycin and teicoplanin among methicillin resistant CoNS isolates from new born blood cultures in a tertiary care centre in Southern India.","authors":"Neethu John, Chinnu Sajeev, Greeshma Hareendranath, Tanya Tonny Mampilly, Aswathy Varghese, Lancy Justus","doi":"10.18502/ijm.v16i5.16792","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16792","url":null,"abstract":"Background and objectives: Coagulase-negative-Staphylococci (CoNS) are important etiological agent of bacteraemia in newborn babies. Methicillin resistant CoNS infections have only limited treatment options. The antimicrobial susceptibility pattern of Methicillin Resistant CoNS isolates from newborn blood cultures was studied with special reference to MICs of Vancomycin and Teicoplanin.Materials and methods: The study population included Methicillin Resistant Coagulase Negative Staphylococcal isolates (MRCoNS) from newborn blood cultures, during a one-year period. Minimum inhibitory concentration (MIC) of Vancomycin and Teicoplanin in Methicillin resistant CoNS isolates was determined by macrobroth dilution method as per CLSI guidelines and by automated methods.Results: Coagulase Negative Staphylococci were the etiological agent in 73.7% (n=56) cases of neonatal bacteremia. Methicillin resistance in newborn CoNS was found to be 58.9%. All the MRCoNS isolates had vancomycin and teicoplanin MICs in the susceptible range. There were MRCoNS isolates with MICs in the upper limit of susceptible range for both vancomycin and teicoplanin, which can result in poor clinical response.Conclusion: Continuous large scale multi-centre surveillance studies with special attention to study the MIC pattern of the high-end anti-MRSA agents like vancomycin, teicoplanin, linezolid are to be carried out. This will help the clinicians to judiciously prescribe the antibiotics, which is very essential for antimicrobial stewardship.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"598-604"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus dysgalactiae subsp.-equisimilis as an emerging secondary pathogen in leprosy foot ulcers. 麻风脚溃疡中新出现的继发病原体--赤痢链球菌亚种（Streptococcus dysgalactiae subsp.-equisimilis）。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16795

Kumar Ebineshan, Michael Sukumar Pallapati, Aparna Srikantam

{"title":"Streptococcus dysgalactiae subsp.-equisimilis as an emerging secondary pathogen in leprosy foot ulcers.","authors":"Kumar Ebineshan, Michael Sukumar Pallapati, Aparna Srikantam","doi":"10.18502/ijm.v16i5.16795","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16795","url":null,"abstract":"Background and objectives: Leprosy foot ulcers (LFU) tend to become chronic due to secondary bacterial infections, leading to subsequent disfigurement and disability. Treatment modality for infected plantar ulcers thus so far is majorly based on conventional approach of empirical antibacterial therapy. However, this approach tends to overlook unconventional pathogens which are likely to be present in the LFU.Materials and methods: Twenty-six leprosy patients (17 males and 9 females) who had completed multidrug therapy (MDT) and those are suffering from foot ulcer were included. Using sterile cotton swabs, two wound swabs were collected, of these; one for bacterial culture and another for NGS (Next Generation Sequencing).Results: Out of 26 samples tested on conventional bacterial culture, Streptococcus spp. (50%) was predominant organism. On NGS, 09/26 (34.61%) showed Streptococcus-dysgalactiae-subsp.-equisimilis-GGS 12 as the most abundant single organism, along with some unknown and unclassified organisms; 03/26 (11.5%) were Arcanobacterium-haemolyticum-DSM-20595 alone and 02/26 (7.69%) were Streptococcus-pyogenes alone.Acombination of Arcanobacterium-haemolyticum-DSM-20595 and Streptococcus-dysgalactiae-subsp.-equisimilis-GGS 124 was found in nine (34.61%) specimens.Conclusion: Polymicrobial infection with conventional and unconventional pathogenic bacteria is another notable finding suggesting appropriate interventions. The study findings also reiterate the need for understanding the polymicrobial infections and their role in the clinical progression of the LFU.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"624-630"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0