Iranian Journal of Microbiology最新文献_第4页

In vitro inhibition of Streptococcus mutans by probiotic yogurt fortified with Lactobacillus paracasei and Sargassum angustifolium protein hydrolysate: a functional yogurt for teeth decay prevention. 副干酪乳杆菌和马尾藻蛋白水解物强化益生菌酸奶对变形链球菌的体外抑制作用：一种预防蛀牙的功能性酸奶。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI: 10.18502/ijm.v17i3.18825

Sara Jafarirad, Leila Nateghi, Masoumeh Moslemi, Kian Pahlevan Afshari, Ali Ahmadi Hassan Abad, Kianoush Khosravi-Darani

{"title":"In vitro inhibition of Streptococcus mutans by probiotic yogurt fortified with Lactobacillus paracasei and Sargassum angustifolium protein hydrolysate: a functional yogurt for teeth decay prevention.","authors":"Sara Jafarirad, Leila Nateghi, Masoumeh Moslemi, Kian Pahlevan Afshari, Ali Ahmadi Hassan Abad, Kianoush Khosravi-Darani","doi":"10.18502/ijm.v17i3.18825","DOIUrl":"10.18502/ijm.v17i3.18825","url":null,"abstract":"Background and objectives: Probiotic yogurts enriched with Lactobacillus paracasei (L. paracasei), protein hydrolysates derived from Sargassum angustifolium macroalgae (SAPH), and encapsulated SAPH were formulated to inhibit Streptococcus mutans (S. mutans), the primary bacterium responsible for dental caries.Materials and methods: The yogurt samples were evaluated for physicochemical, microbiological, and sensory characteristics.Results: On day 21, the yogurt supplemented with L. paracasei demonstrated the greatest titratable acidity (97.35°D), the lowest pH value (4.24), reduced syneresis, and enhanced antioxidant, antihypertensive, and rheological properties. In terms of antibacterial activity, the lowest S. mutans count was detected in formulations containing free SAPH, either alone or in combination with L. paracasei. Conversely, yogurts formulated with encapsulated SAPH exhibited higher survival rates of both L. paracasei and S. mutans compared to those containing the free form of SAPH.Conclusion: The findings indicated that although the probiotic yogurt containing free SAPH was more effective in reducing S. mutans levels within the yogurt matrix, the encapsulated form achieved an acceptable level of antibacterial activity while contributing to improved sensory acceptance.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"420-433"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotypic diversity of Mycobacterium tuberculosis strains collected from immigrant patients in Mashhad, Iran using MIRU-VNTR method. 应用MIRU-VNTR方法检测伊朗马什哈德移民患者结核分枝杆菌的基因型多样性

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI: 10.18502/ijm.v17i3.18817

Mahbubeh Jangi, Kiarash Ghazvini, Saman Soleimanpour, Mahdis Ghavidel, Gholamreza Hashemitabar

{"title":"Genotypic diversity of Mycobacterium tuberculosis strains collected from immigrant patients in Mashhad, Iran using MIRU-VNTR method.","authors":"Mahbubeh Jangi, Kiarash Ghazvini, Saman Soleimanpour, Mahdis Ghavidel, Gholamreza Hashemitabar","doi":"10.18502/ijm.v17i3.18817","DOIUrl":"10.18502/ijm.v17i3.18817","url":null,"abstract":"Background and objectives: This research aimed to explore the genetic diversity and phylogenetic relationships of Mycobacterium tuberculosis (Mtb) strains, as well as to assess their drug susceptibility, specifically in strains isolated from immigrant patients attending the Referral Tuberculosis Laboratory in Mashhad.Materials and methods: A total of 52 sputum samples isolated from patients were examined utilizing the Mycobacterial Interspersed Repetitive-Unit Variable Number of Tandem Repeats (MIRU-VNTR). Drug-susceptibility testing against rifampin (RIF) and isoniazid (INH) was measured utilizing the proportional strategy. Thereafter, for more examination, Xpert MTB/RIF and multiplex allele-specific PCR (MAS-PCR) was performed to determine RIF and INH-resistance within the Mtb strains.Results: Among 52 Mtb isolates, 2 (3.8%) were resistant to rifampin and one isolate was resistant to both INH and RIF and considered as multidrug-resistance (MDR) isolate. According to MIRU-VNTR, the most prominent genetic-variation patterns of these samples, were related to NEW-1 (n=18, 34.6%), followed by CAS/Delhi (n=17, 32.7%), Haarlem (n=12, 23%), Uganda I (n=2, 3.8%), S (n=1, 1.9%), Beijing (n=1, 1.9%), and unknown (n=1, 1.9%) genotypes. The statistical analysis showed that the estimated percentage of the recent TB-transmission in this study was 0.21%.Conclusion: The result of this study indicated a great diversity of MTBC circulating among Afghan-immigrants which might be one of the reasons for the infection to become active. The relatively high percentage of resistant isolates in the studied population shows the importance of screening the immigrants especially at the entry borders and treatment and follow up of patients, to control TB-incidence in country.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"358-365"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized isolation and purification of native glycoprotein B from herpes simplex virus 1: a streamlined approach. 单纯疱疹病毒1型天然糖蛋白B的优化分离纯化：一种简化的方法。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI: 10.18502/ijm.v17i3.18833

Mohammad Yasaghi, Ahad Yamchi, Alijan Tabarraei, Sara Salari, Abdolvahab Moradi, Seyedeh Delafruz Hosseini

{"title":"Optimized isolation and purification of native glycoprotein B from herpes simplex virus 1: a streamlined approach.","authors":"Mohammad Yasaghi, Ahad Yamchi, Alijan Tabarraei, Sara Salari, Abdolvahab Moradi, Seyedeh Delafruz Hosseini","doi":"10.18502/ijm.v17i3.18833","DOIUrl":"10.18502/ijm.v17i3.18833","url":null,"abstract":"Background and objectives: Viral membrane glycoproteins are essential for host cell recognition, membrane fusion and immune evasion, making them critical targets for antiviral therapies and vaccine development. However, their isolation in native conformation is challenging due to structural complexity and limitations of conventional purification methods. The aim of current study was to develop a cost-effective, reproducible method for the isolation and purification of glycoprotein B (gB) from Herpes Simplex Virus type 1 (HSV-1) while maintaining its native conformation for functional and interaction studies.Materials and methods: HSV-1 particles were concentrated via ultracentrifugation and membrane proteins were extracted using a modified protocol of the Mem-PER™ Plus Membrane Protein Extraction Kit. Native PAGE with a 4-8% gradient gel was employed to isolate multimeric gB (~300 kDa), followed by electroelution to extract the protein from the gel. The purity and integrity of gB were validated using SDS-PAGE and Western blot analysis.Results: The method successfully isolated glycoprotein B in its native multimeric form with high purity and adequate concentration (0.157 mg/mL). The pH of the native gel (8.3) and the high molecular weight of gB facilitated separation from other viral surface proteins. SDS-PAGE and Western blot confirmed the specificity and structural integrity of the purified protein.Conclusion: This study introduces a cost-effective and reliable method for isolating viral glycoproteins in their native conformation. The approach offers significant advantages over traditional chromatography-based techniques, making it ideal for research-scale applications, including functional and interaction studies.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"503-510"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outpatient crystalluria: prevalence, crystal types, and associations with comorbidities and urinary tract infections at a provincial hospital. 门诊结晶尿：某省医院结晶尿患病率、结晶类型及其与合并症和尿路感染的关系

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI: 10.18502/ijm.v17i3.18820

Samira Natoubi, Rim Jamal, Nezha Baghdad

{"title":"Outpatient crystalluria: prevalence, crystal types, and associations with comorbidities and urinary tract infections at a provincial hospital.","authors":"Samira Natoubi, Rim Jamal, Nezha Baghdad","doi":"10.18502/ijm.v17i3.18820","DOIUrl":"10.18502/ijm.v17i3.18820","url":null,"abstract":"Background and objectives: Crystalluria refers to the occurrence of crystals in urine resulting from urinary supersaturation, which disrupts the balance between factors that promote and those that inhibit crystal formation in urine. This study aimed to assess the prevalence of crystalluria, identify crystal types, determine associated comorbidities, and assess links with bacterial urinary tract infections in outpatients at Hassan II Hospital in Settat.Materials and methods: A retrospective study was conducted from January 2022 to May 2023 at Hassan II Hospital. Urine samples from patients suspected of urinary tract infections, who underwent cytobacteriological urine examinations, were analyzed.Results: Among 1,025 urine samples, 22.04% showed crystalluria. The mean age of patients was 51.3 with a standard deviation of 18.1 years. The most common crystal types were calcium oxalate (46.4%), uric acid (23.5%), urates (15.1%) and struvite (9.3%). Comorbidities including, diabetes, kidney failure, prostatitis, and nephrotic syndrome was associated with urinary crystal formation. The prevalence of urinary tract infections in patients with urinary crystals was 10.6%. Struvite crystals were specifically associated with bacterial infections, especially with Proteus mirabilis, Escherichia coli, Citrobacter freundii, Citrobacter koseri, and Enterobacter cloacae.Conclusion: Monitoring urinary crystals is essential for preventing the formation of kidney calculi and crystal-associated infections, especially in high-risk individuals.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"382-389"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of Helicobacter pylori infection among dyspeptic patients attending Baghdad medical city complex. 在巴格达医疗城综合医院就诊的消化不良患者中幽门螺杆菌感染的流行情况。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI: 10.18502/ijm.v17i3.18822

Basim M Ibrahim, Huda Saad Salman, Mohammed Mazin Mohammed, Hala Mohammed Mjeed

{"title":"Prevalence of Helicobacter pylori infection among dyspeptic patients attending Baghdad medical city complex.","authors":"Basim M Ibrahim, Huda Saad Salman, Mohammed Mazin Mohammed, Hala Mohammed Mjeed","doi":"10.18502/ijm.v17i3.18822","DOIUrl":"10.18502/ijm.v17i3.18822","url":null,"abstract":"Background and objectives: Dyspepsia is a disorder characterized by difficulty in digestion and represents a major health concern. Therefore, it is crucial to identify functional dyspepsia linked to Helicobacter pylori (H. pylori). This research aimed to determine the prevalence of H. pylori among patients with dyspepsia and to examine the potential risk factors associated with the infection.Materials and methods: From August 14th to September 21st, 2024, a total of 105 patients with dyspepsia, who attended the Central Laboratory of Baghdad Medical City Complex (Iraq), were enrolled in this study. Data on nonsteroidal anti-inflammatory drugs (NSAIDs), smoking, family history, fasting habits and frequent fast food consumption were collected through participant interviews.Results: Based on the urea breath test results, dyspeptic patients were categorized into infected (63.8%) and non-infected (36.2%) groups. Factors that influenced these patients included the intake of NSAIDs (48.6%), smoking (21.9%), family history (29.5%), fasting habits (36.2%) and regular consumption of fast food (57.1%).Conclusion: Dyspeptic patients exhibit a high prevalence of H. pylori infection, indicating the significant impact of H. pylori on this population. However, the intake of NSAIDs, smoking, family history, fasting habits and regular fast food consumption have no significant effects on the presence of H. pylori.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"397-403"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The inhibitory effect of the methanolic extract and the essence of Anvillea garcinii on expression of the genes related to Staphylococcus aureus biofilm formation. 黄芪甲醇提取物和黄芪精华对金黄色葡萄球菌生物膜形成相关基因表达的抑制作用。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI: 10.18502/ijm.v17i3.18830

Maryam Ansari, Mohammad Kazemi, Maryam Mohammadi Sichani, Vajihe Karbasizade

{"title":"The inhibitory effect of the methanolic extract and the essence of Anvillea garcinii on expression of the genes related to Staphylococcus aureus biofilm formation.","authors":"Maryam Ansari, Mohammad Kazemi, Maryam Mohammadi Sichani, Vajihe Karbasizade","doi":"10.18502/ijm.v17i3.18830","DOIUrl":"10.18502/ijm.v17i3.18830","url":null,"abstract":"Background and objectives: Staphylococcus aureus (S. aureus) is a pathogenic bacterium whose virulence is attributed to its extracellular compounds and biofilm-forming ability. This study aimed to evaluate the inhibitory effects of the methanolic extract (AGME) and the essential oil (AGEO) of Anvillea garcinii on the growth and the biofilm formation of S. aureus.Materials and methods: The antibacterial and antibiofilm activities of AGME and AGEO against S. aureus ATCC 6538 were assessed using the microbroth dilution method and the Crystal Violet Staining Assay, respectively. The expression levels of sarA, spa, and icaA, genes involved in biofilm formation, were analyzed using real-time PCR.Results: AGME and AGEO inhibited S. aureus growth at minimum inhibitory concentrations (MIC) of 1 mg/ml and 0.6 mg/ml, respectively. AGME exhibited a 72% inhibition of biofilm formation at ¼ MIC, whereas AGEO showed no significant antibiofilm activity. AGME downregulated the expression of sarA, a key regulator of biofilm formation, as well as spa, and icaA genes.Conclusion: This study demonstrated that A. garcinii essential oil (AGEO) exhibits significant antimicrobial activity, while its methanolic extract (AGME) effectively inhibits biofilm formation in S. aureus. These findings suggest the potential application of AGEO and AGME as antimicrobial and antibiofilm agents. Further investigations on their efficacy against other bacterial pathogens are recommended.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"470-479"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro effects of purified lacticin from whey isolated Lactococcus lactis culture on Staphylococcus aureus and MCF-7 breast cancer cells. 乳清分离乳酸乳球菌培养物纯化乳蛋白对金黄色葡萄球菌和MCF-7乳腺癌细胞的体外作用

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI: 10.18502/ijm.v17i3.18828

Mahtab Hosseinzadeh, Monir Doudi, Ali Mohammad Ahadi

{"title":"In vitro effects of purified lacticin from whey isolated Lactococcus lactis culture on Staphylococcus aureus and MCF-7 breast cancer cells.","authors":"Mahtab Hosseinzadeh, Monir Doudi, Ali Mohammad Ahadi","doi":"10.18502/ijm.v17i3.18828","DOIUrl":"10.18502/ijm.v17i3.18828","url":null,"abstract":"Background and objectives: Bacteriocins are interested as antibacterial and anticancer agents due to their high specificity and low side effects. This study aimed to isolate bacteria which produce bacteriocins of the lacticin family from whey and to investigate their antibacterial and anticancer effects.Materials and methods: Lactic acid bacteria were isolated from different whey samples. The presence of the lacticin gene in the isolates was checked using PCR and then the inhibitory effects of their bacteriocin was investigated on Staphylococcus aureus utilizing well plate method. The protein content was separated by dialysis. The presence of lacticin was checked with the help of SDS-PAGE. The lacticin producing bacterium was identified through the sequencing of the 16S rRNA gene. Finally, the cytotoxicity of the obtained protein was studied on the MCF-7 breast cancer cells using MTT and scratching tests.Results: The isolated lacticin-producing Lactococcus lactis was able to grow in acidic conditions (pH = 2.5 for 3 h) and in bile salts (0.3% for 24 h). The bacterium produced 4.2 μg/μl bacteriocin with a molecular weight of 3.1 KD. The lacticin showed antibacterial effect against S. aureus. The cancerous cells treated with lacticin had slower growth than the control in Scratch test. Based on the MTT results, more than 80% of cancerous cells were inhibited at a concentration of 7 μg/ml lacticin with IC = 5.2 μg/ml.Conclusion: The bacteriocin produced in this study is a promising antibacterial and anticancer agent.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"451-459"},"PeriodicalIF":1.3,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and assessment of a multiplex real-time PCR method for simultaneous detection and differentiation of COVID-19 and Influenza A/B. 新型冠状病毒病与甲型流感/乙型流感同时检测与鉴别的多重实时PCR方法设计与评价

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI: 10.18502/ijm.v17i2.18381

Nafiseh Fotros, Reihaneh Bashiri, Samira Mohammadi-Yeganeh, Mahdi Paryan

{"title":"Design and assessment of a multiplex real-time PCR method for simultaneous detection and differentiation of COVID-19 and Influenza A/B.","authors":"Nafiseh Fotros, Reihaneh Bashiri, Samira Mohammadi-Yeganeh, Mahdi Paryan","doi":"10.18502/ijm.v17i2.18381","DOIUrl":"https://doi.org/10.18502/ijm.v17i2.18381","url":null,"abstract":"Background and objectives: Viral infections of the respiratory system are a major public problem due to their ease of spread, pandemic potential, and significant rate of death. Diagnosing these infections requires laboratory testing, as clinical symptoms alone are often insufficient. Influenza A, Influenza B, and COVID-19 are common infections that burden the population, especially during winter. We developed a multiplex real-time PCR method to simultaneously detect Influenza A and B, as well as COVID-19. Compared to existing detection kits, it offers higher accuracy, lower costs, and faster results, making it an efficient diagnostic tool.Materials and methods: We designed primer/TaqMan probes for the M2 gene of Influenza A, N gene of SARS-CoV-2, and NS1 gene of Influenza B. Reaction components were optimized and functional parameters were tested using standard samples with known viral copy numbers.Results: The method's detection limit is 10 copies for Influenza A and B, and 60 for SARS-CoV-2. Sensitivity and specificity for Influenza A are 88% and 100%, for Influenza B, 95.6% and 100%, and for SARS-CoV-2, 90.4% and 100%.Conclusion: This multiplex real-time PCR method can accurately detect and distinguish SARS-CoV-2, Influenza B, and Influenza A infections.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"204-210"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the susceptibility to ceftazidime-avibactam in clinical isolates of Klebsiella pneumoniae and Pseudomonas aeruginosa recovered from an apex medical hospital in north India. 评价印度北部某顶级医院临床分离肺炎克雷伯菌和铜绿假单胞菌对头孢他啶-阿维巴坦的敏感性。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI: 10.18502/ijm.v17i2.18385

Nargis Bali, Tufail Ahmed, Biswajyoti Borkakoty, Roseleen Bali, Anjum Ara Mir, Zubair Teli, Qounser Nisar, Tantray Faisal

{"title":"Evaluating the susceptibility to ceftazidime-avibactam in clinical isolates of Klebsiella pneumoniae and Pseudomonas aeruginosa recovered from an apex medical hospital in north India.","authors":"Nargis Bali, Tufail Ahmed, Biswajyoti Borkakoty, Roseleen Bali, Anjum Ara Mir, Zubair Teli, Qounser Nisar, Tantray Faisal","doi":"10.18502/ijm.v17i2.18385","DOIUrl":"10.18502/ijm.v17i2.18385","url":null,"abstract":"Background and objectives: We assessed the susceptibility of ceftazidime+avibactam (CZA/AVI) in Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from intensive care units of our hospital.Materials and methods: Clinical samples from Jan 2022 to Dec 2023 at SKIMS Soura, were processed for the recovery of K. pneumoniae and P. aeruginosa. Susceptibility testing was done by disc diffusion (DD) method and minimum inhibitory concentration (MIC) for CZA/AVI and meropenem was assessed using E-test strips. Categorical agreement (CA), very major errors (VME), major errors (ME) and minor errors (mE) between DD and MIC were measured. Statistical analyses were performed using SPSS version 22.0.Results: A total of 111 K. pneumoniae and 81 P. aeruginosa were part of the study. Of these, 56.8% K. pneumoniae and 45.7% P. aeruginosa isolates were susceptible to CZA/AVI. MIC of CZA/AVI for K. pneumoniae ranged from 0.125 to ≥ 256 μg/ml and for P. aeruginosa it ranged from 0.032 to 128 μg/ml. CA was 97.29% between DD and E-Test for CZA/AVI in K. pneumoniae isolates, with a ME of 2.70%. For P. aeruginosa CA between DD and E-Test for CZA/AVI was 98.76% with a VME of 1.23%. MIC values of meropenem were higher than CZA/AVI even in sensitive isolates.Conclusion: CZA/AVI shows good in-vitro activity against clinical isolates of K. pneumoniae and P. aeruginosa and can be part of empirical therapy for treating infections caused by these bacteria.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"253-260"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and genotypic characterization of carbapenemase-producing Escherichia coli clinical isolates in Thi-Qar, Iraq. 伊拉克Thi-Qar产碳青霉烯酶大肠杆菌临床分离株的表型和基因型特征

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI: 10.18502/ijm.v17i2.18387

Moslim Mohsin Khalaf, Firas Srhan Abd Al-Mayahi

{"title":"Phenotypic and genotypic characterization of carbapenemase-producing Escherichia coli clinical isolates in Thi-Qar, Iraq.","authors":"Moslim Mohsin Khalaf, Firas Srhan Abd Al-Mayahi","doi":"10.18502/ijm.v17i2.18387","DOIUrl":"https://doi.org/10.18502/ijm.v17i2.18387","url":null,"abstract":"Background and objectives: The emergence of carbapenem resistance in Escherichia coli (E. coli) poses an urgent threat. The study aims to assess carbapenem resistance and the presence of carbapenemase genes in E. coli clinical isolates from Thi-Qar Hospital, Iraq.Materials and methods: A total of 2203 specimens were collected from patients at two hospitals between January and October 2024. E. coli was identified via biochemical tests and confirmed with the Vitek2® system. Antibiotic sensitivity was evaluated using disc diffusion, and carbapenemase production was investigated through combined disc tests (CDT) and modified Hodge tests (MHT). PCR was used to detect carbapenemase genes.Results: Out of 2203 specimens, 1212 (55.02%) exhibited bacterial growth, with E. coli accounting for 15.35% (186/1212) of isolates. Among these, 40 (21.51%) were resistant to at least one carbapenem. CDT identified 10, and MHT identified 1 as a carbapenemase producer. The most detected gene was bla NDM (60.00%), followed by bla OXA (40.00%) and bla OXA-48 (15.00%). bla OXA-51 and bla VIM were found in 5.00% of isolates each. No bla KPC, bla NMC, bla IMI, bla GES, bla SPM, bla GIM, or bla SIM was detected.Conclusion: The high prevalence of carbapenem resistance and the corresponding encoding genes in E. coli in Thi-Qar province pose a concerning challenge for managing serious infections caused by this pathogen.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"268-277"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0