Iranian Journal of Microbiology最新文献

{"title":"Determine phenotypical patterns of resistance to antibiotics in COVID-19 patients with associated bacterial infection: largest medical center in Iran.","authors":"Esmaeil Mohammadnejad,&nbsp;Arash Seifi,&nbsp;Reza Ghanei Gheshlagh,&nbsp;Amir Aliramezani,&nbsp;Samrand Fattah Ghazi,&nbsp;Mohammadreza Salehi,&nbsp;Seyed Ali Dehghan Manshadi,&nbsp;Amirhossein Orandi","doi":"10.18502/ijm.v15i3.12893","DOIUrl":"https://doi.org/10.18502/ijm.v15i3.12893","url":null,"abstract":"<p><strong>Background and objectives: </strong>Antibacterial resistance (AMR) is a serious threat and major concern, especially in developing countries. Therefore, we aimed to determine phenotypical patterns of resistance to antibiotics in COVID-19 patients with associated bacterial infection in intensive care units.</p><p><strong>Materials and methods: </strong>In this cross-sectional study, 6524 COVID-19 patients admitted for more than 48 h in the ICUs of Imam Khomeini Complex Hospital (IKCH) in Tehran from March 2020 to January 2022 were included in the study with initial diagnosis of COVID-19 (PCR test and chest imaging). Data were collected regarding severity of the illness, primary reason for ICU admission, presence of risk factors, presence of infection, length of ICU and hospital stay, microbial type, and antibiotic resistance. In this study, the pattern of antibiotic resistance was determined using the Kirby-Bauer disk diffusion method.</p><p><strong>Results: </strong>In this study, 439 (37.5%) were ventilator-related events (VAEs), and 46% of all hospitalized patients had an underlying disease. The most common microorganisms in COVID-19 patients were carbapenem resistant <i>Klebsiella pneumoniae</i> (KPCs) (31.6%), <i>Escherichia coli</i> (<i>E. coli</i>) (15.8%), and <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) (15.7%), respectively. Prevalence of vancomycin-resistant enterococci (VRE) and KPCs were 88% and 82%, respectively.</p><p><strong>Conclusion: </strong>A study on AMR surveillance is the need of the hour as it will help centers to generate local antibiograms that will further help formulate national data. It will guide doctors to choose the appropriate empiric treatment, and these studies will be the basis for establishing antimicrobial surveillance and monitoring and regulating of the use of antimicrobials.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"336-342"},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/af/23/IJM-15-336.PMC10336291.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9822956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of genetic and environmental variables on biofilm development dynamics in <i>Achromobacter mucicolens</i>.","authors":"Rusul Al-Kahachi,&nbsp;Sura Al-Asadi,&nbsp;Zainab Oun Ali,&nbsp;Jamila Rampurawala","doi":"10.18502/ijm.v15i3.12902","DOIUrl":"https://doi.org/10.18502/ijm.v15i3.12902","url":null,"abstract":"<p><strong>Background and objectives: </strong>The study aimed to investigate whether <i>Achromobacter mucicolens</i> IA strain biofilm formation, which contributes to antibiotic resistance, could be enhanced by readily available nutrient sources like carbohydrates and environmental factors such as pH and NaCl. Additionally, the study aimed to identify any inherent genes that support biofilm formation in this strain, which is an opportunistic pathogen that affects immunocompromised patients and is resistant to many antibiotics.</p><p><strong>Materials and methods: </strong>Biofilm growth in different carbohydrate, pH, and NaCl concentrated media was measured using crystal violet microtiter assay. All the treatments were subjected to biostatistics analysis for normality, Test of Homogeneity, one way ANOVA analysis. Whole-genome sequencing of our IA strain was conducted to identify various gene sequences.</p><p><strong>Results: </strong>Biofilm formation was measured at different carbohydrate concentrations, and the optimum biofilm formation was observed at 3M glucose and 0.5M NaCl, while the lowest results were seen at 2M maltose concentration. Whole-genome sequencing identified potential genes involved in biofilm formation, pathogenicity, protein metabolism, flagellar motility, cell wall component synthesis, and a multidrug efflux pump.</p><p><strong>Conclusion: </strong>These findings suggest that biofilm formation is influenced by extrinsic and intrinsic factors, which could aid in the development of effective treatments for resistant infections.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"414-424"},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bd/c3/IJM-15-414.PMC10336289.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10180916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological characteristics and HPV status in cervical biopsy specimens diagnosed as flat condyloma.","authors":"Fatemeh Nategh,&nbsp;Mitra Mohit,&nbsp;Minoo Saatian,&nbsp;Zohreh Farahmand,&nbsp;Hoorieh Soleimanjahi","doi":"10.18502/ijm.v15i3.12909","DOIUrl":"https://doi.org/10.18502/ijm.v15i3.12909","url":null,"abstract":"<p><strong>Background and objectives: </strong>HPV infections cause a wide spectrum of pathological changes in lower anogenital epithelium. The aim of this study was to investigate the HPV DNA status and histological findings in cervical biopsy specimens diagnosed as flat condyloma.</p><p><strong>Materials and methods: </strong>This study included 20 cervical biopsy specimens diagnosed as flat condyloma. The histopathological criteria and presence of HPV DNA were evaluated. HPV genotyping was determined in HPV-positive specimens using BioEdit software and the results were analyzed in SPSS software.</p><p><strong>Results: </strong>HPV DNA was not found in 30% of specimens and relative frequency of HPV genotypes was: 15% HPV6, 15% HPV11, 5% HPV16, 5% HPV18, 5% HPV53, 5% HPV68, 5% HPV84, 10% HPV45. Relative frequency of histopathological criteria was as below: 100% of specimens had koilocytosis, 100% acanthosis, 15% nuclear immaturity, 100% atypia, 15% mitotic activity, 50% dyskeratosis, 35% parakeratosis and 10% hyperkeratosis.</p><p><strong>Conclusion: </strong>There were significant differences between HPV positivity and two pathologic criteria; multinucleation and hyperkeratosis (P Value: 0.02). Nuclear immaturity was significantly more prevalent in high risk HPV-positive specimens (P Value: 0.03).</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"468-474"},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/46/96/IJM-15-468.PMC10336288.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10199029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequent detection and genotyping of human rhinovirus in SARS-CoV-2 negative patients; a study from south of Iran.","authors":"Amirhossein Darabi,&nbsp;Behrang Sarshari,&nbsp;Katayoun Vahdat,&nbsp;Seyed Alireza Mousavi,&nbsp;Mohsen Keshavarz","doi":"10.18502/ijm.v15i3.12908","DOIUrl":"https://doi.org/10.18502/ijm.v15i3.12908","url":null,"abstract":"<p><strong>Background and objectives: </strong>Human rhinovirus (HRV), a major cause of common cold, was associated to the hospitalization of children and adults. This cross-sectional study aimed to determine the prevalence, and genotype distribution of HRV in the patients with mild to severe respiratory infections who were negative for SARS-Cov-2.</p><p><strong>Materials and methods: </strong>Nasopharyngeal swab specimens (n = 356) from the patients aged 29 days to 82 years, received for the respiratory virus detection from January to December 2021, were analyzed for human rhinovirus (HRV) by RT-PCR. As a final step, genotyping was performed on obtained sequences.</p><p><strong>Results: </strong>A total of 37 HRV infections were identified (37/356, 10%). The highest rates of positive HRV tests were observed in February (21.6%), and January (18.9%), compared with June and August (0%). HRV-positive cases mainly appeared in winter. Among the age groups, those 2-<5 years of age had the highest detection rate (21%), however, those >55 years of age had the lowest detection rate (3%). Among HRV-positive samples, 30 (81%) were identified as type HRV-A, 5 (13.5%) as HRV-B, and 2 (5.5%) as HRV-C.</p><p><strong>Conclusion: </strong>Our results suggested that HRV frequency gradually decreased with the age of patients which is more active in Iran, especially in the cold months.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"462-467"},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bf/da/IJM-15-462.PMC10336278.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10199023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The high cross-transmission in methicillin resistant <i>Staphylococcus aureus</i> between healthy and patient communities.","authors":"Behnood Haji Sheikhzadeh,&nbsp;Leila Rahbarnia,&nbsp;Alireza Dehnad,&nbsp;Behrooz Naghili,&nbsp;Parvaneh Saffarian","doi":"10.18502/ijm.v15i3.12901","DOIUrl":"https://doi.org/10.18502/ijm.v15i3.12901","url":null,"abstract":"Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of high mortality and morbidity in hospitals. This study was aimed to examine virulence factors, molecular typing, and the antibiotic resistance pattern of MRSA isolates in hemodialysis patients and healthy communities. Materials and Methods: Total of 231 and 400 nasal samples were obtained from hemodialysis patients and healthy communities, respectively. Virulence factors profile was examined in two groups by PCR reaction. Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) was used as a molecular typing approach. Results: Overall, 35.49% (82/231) of hemodialysis patients were positive for S. aureus, and 47.56% (39/82) of isolates were positive for mecA. In a healthy community, 15% (60/400) of samples were positive for S. aureus, and 36.66% (22/60) were positive for mecA. The frequency of MDR was significantly higher in patients group (p-value < 0.00001). The frequency of pvl (p.value = 0.003932, P<0.05) and tsst-1 (p.value = 0.003173, p < .05) were significantly higher in patients group. The highest frequency virulence factors in healthy individuals were related to hla (68.33%, 41/60), hlb (53.33%, 32/60), and Acme/arcA (46.66%) genes. Two groups were clustered by the ERIC-PCR method into 7 clusters and 2 single isolate with a 0.74 similarity index. Based on the results, each cluster was combination with healthy and patient isolates. Conclusion: Our findings indicate a notable variation in the frequency of virulence factors between S. aureus isolates obtained from dialysis patients and the healthy community.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"408-413"},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f2/6f/IJM-15-408.PMC10336293.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9819962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High burden of MDR, XDR, PDR, and MBL producing Gram negative bacteria causing infections in Kermanshah health centers during 2019-2020.","authors":"Sepide Kadivarian,&nbsp;Mosayeb Rostamian,&nbsp;Shirin Dashtbin,&nbsp;Sara Kooti,&nbsp;Zahra Zangeneh,&nbsp;Ramin Abiri,&nbsp;Amirhooshang Alvandi","doi":"10.18502/ijm.v15i3.12896","DOIUrl":"https://doi.org/10.18502/ijm.v15i3.12896","url":null,"abstract":"<p><strong>Background and objectives: </strong>Microorganisms producing Metallo-Beta-Lactamase (MBL) are a threat and cause of concern as they have become one of the most feared resistance mechanisms. This study was designed to explore the prevalence of MBL production in clinical isolates of Gram negative bacteria using phenotypic MBL detection.</p><p><strong>Materials and methods: </strong>A total of 248 isolates were collected from various clinical samples and were evaluated for carbapenem resistance and MBL production. All strains were screened for MBL production using Double Disk Confirmatory Test (DDCT).</p><p><strong>Results: </strong>The results of screening for MBL production using phenotypic disk diffusion method showed that in the 85 isolates were carbapenemase positive; including, 10 (16.1%) <i>Klebsiella pneumoniae</i>, 9 (14.5%) <i>Escherichia coli</i>, 58 (93.6%) <i>Acinetobacter baumannii</i>, and 8 (12.9%) <i>Pseudomonas aeruginosa</i> isolates. Also, 83 (97.6) Carbapenemase-producing isolates were resistant to at least four classes of antimicrobials (MDR).</p><p><strong>Conclusion: </strong><i>A. baumannii</i> was the most common carbapenem resistant bacterium in medical centers in Kermanshah. Significant multiple drug resistance (MDR) incidence was observed compared to different classes of antibiotics.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"359-372"},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/97/ea/IJM-15-359.PMC10336284.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9813414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal and bacterial co-infection in the superficial and deep sternal wound after open cardiac surgery.","authors":"Gholamali Riahi,&nbsp;Ebrahimeh Koohsari,&nbsp;Seyed Sedigheh Hosseini","doi":"10.18502/ijm.v15i3.12899","DOIUrl":"https://doi.org/10.18502/ijm.v15i3.12899","url":null,"abstract":"Background and Objectives: Sternum infection increases the time of the patients stay in the hospital and, as a result, increases the treatment costs. This study aimed to evaluate the fungal and bacterial co-infection in the superficial and deep sternal wounds after open cardiac surgery and its relationship with risk factors, as sternal infection increases the time of the patient's stay in the hospital and, as a result, increases the treatment costs. Materials and Methods: Data were collected using a questionnaire and sampling with two swabs after open heart surgery and hospitalization from 21 March 2018 to 20 March 2019 and sent to the laboratory for diagnosis of microorganisms effective in wound infection. Susceptibility testing for fluconazole and specific antibiotics was performed by the disk diffusion method. Results: Out of 210 patients studied, 2% of patients had deep sternal wound infections. The most common coinfection fungal and bacterial agents in sternal wounds were caused by Staphylococcus aureus with Candida glabrata 4% and Escherichia coli with Candida albicans 2%. S. aureus and E. coli showed the highest antibiotic susceptibility to the antibiotics ciprofloxacin, norfloxacin, meropenem, and imipenem. Candida glabrata and Candida albicans had the highest rate of resistance to fluconazole. Conclusion: According to the results of this study, patients on the 7th day in the cardiac care unit (CCU) and the 28th day are at higher risk of getting confection of fungi with bacteria in the sternal wound. Therefore, timely and appropriate antibiotic therapy, including the use of appropriate antibiotics, can be an important step in the patient's recovery.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"392-400"},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/63/a3/IJM-15-392.PMC10336292.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9819963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of 17 β-estradiol by <i>Serratia marcescens</i> and <i>Stenotrophomonas tumulicola</i> co-culture isolated from a sewage treatment plant in Upper Egypt.","authors":"Ibrahim Mahmoud Ali,&nbsp;Hamada Mohamed Mohamed Halby,&nbsp;Bahaa-Eldin Anwar Abd-Elrady,&nbsp;Mohamed Taha Salim,&nbsp;Heba Ahmed Mohamed","doi":"10.18502/ijm.v15i3.12906","DOIUrl":"https://doi.org/10.18502/ijm.v15i3.12906","url":null,"abstract":"<p><strong>Background and objectives: </strong>17 β-estradiol (E2) is an important pollutant of the aquatic system. It is responsible for sexual disruptions in the majority of aquatic organisms. This study aimed to search for bacteria with high potential degradation of E2 as an important method for bioremediation.</p><p><strong>Materials and methods: </strong>Sewage water samples were collected and treated to isolate bacterial strains which were identified by conventional methods and 16S ribosomal RNA gene sequence analysis. The biodegradation of E2 by the isolated strains was evaluated under different environmental conditions.</p><p><strong>Results: </strong>Two bacterial strains were recovered from sewage water samples and identified as <i>Stenotrophomonas tumulicola</i> and <i>Serratia marcescens</i>, (named ASc2 and ASc5 respectively). Co-culture of the two strains showed biodegradation of approximately 93.6 % of E2 (50 mg. L^-1) within 48 hours. However, the biodegradation capacity of the same E2 concentration was 69.4% and 71.2% for ASc2 and ASc5 each alone, respectively. The optimum cultivation conditions for efficient E2 biodegradation by co-culture were 5% (v/v) inoculation volume with 50 mg. L^-1 of E2 as the initial concentration at pH 7 and 30°C within 48 hours inoculation period.</p><p><strong>Conclusion: </strong>This study detected new bacterial strains that are capable of rapid degradation of estrogen as an environmental pollutant.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 3","pages":"448-455"},"PeriodicalIF":1.4,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/86/c5/IJM-15-448.PMC10336294.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9819958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant growth promoting and antagonistic traits of bacteria isolated from forest soil samples.","authors":"Lavudya Bindu Chowhan,&nbsp;Mohammad Imran Mir,&nbsp;Mayada A Sabra,&nbsp;Ahmed A El-Habbab,&nbsp;B Kiran Kumar","doi":"10.18502/ijm.v15i2.12480","DOIUrl":"https://doi.org/10.18502/ijm.v15i2.12480","url":null,"abstract":"<p><strong>Background and objectives: </strong>Sustainability in agricultural systems without compromising the environmental quality and conservation is one of the major concerns of today's world. The excessive use of agrochemicals is posing serious threats to the environment. Therefore identification of efficient plant growth promoting (PGP) bacteria as an alternative to chemically synthesized fertilizers is of great interest.</p><p><strong>Materials and methods: </strong>In the present investigation, forest soil samples collected were used for isolation of efficient plant growth promoting bacteria.</p><p><strong>Results: </strong>Total of 14 bacteria were isolated, and tested for various PGP properties. Out of the 14 isolates, four isolates labelled as BKOU-1, BKOU-8, BKOU-13 and BKOU-14 showed significant plant growth promoting traits, hydrolytic enzyme production and effectively restricted the mycelial development of phyto-pathogenic fungi (<i>Fusarium oxysporum</i> and <i>Macrophomina phaseolina</i>). 16 S rRNA gene sequences of the bacterial isolates BKOU-1, BKOU-8, BKOU-13 and BKOU-14 were found to have maximum identity with <i>Bacillus aerius, Bacillus infantis, Alcaligenes faecalis</i> and <i>Klebsiella Oxytoca</i> respectively. All four bacterial isolates nucleotide sequences were submitted to GenBank and NCBI accession numbers were generated as follows: OL721916, OL721918, OL721919 and OL721926.</p><p><strong>Conclusion: </strong>According to the findings of the study, these PGPR could be employed as biofertilizers/biopesticides to boost crop yield of different crops in sustainable manner.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 2","pages":"278-289"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f3/50/IJM-15-278.PMC10183078.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9540871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The acute effects of nonstructural-1 protein dengue virus type 2 on wet liver weight, zonulin expression and serum zonulin.","authors":"Safari Wahyu Jatmiko,&nbsp;Hartono Hartono,&nbsp;Tonang Dwi Ardyanto,&nbsp;Dono Indarto","doi":"10.18502/ijm.v15i2.12483","DOIUrl":"https://doi.org/10.18502/ijm.v15i2.12483","url":null,"abstract":"<p><strong>Background and objectives: </strong>Intestinal leakage commonly occurs in severe dengue infection with zonulin as a biomarker. The aim of this study was to determine the effects of NS1 on liver weight, zonulin expression and serum zonulin levels.</p><p><strong>Materials and methods: </strong>This laboratory experiment used 18 ddY mice, which were randomly divided into control (C), PBS (T1), and PBS + NS1 (T2) groups. Mice in the T1 and T2 groups were intravenously injected with 500 μl PBS only and 50 μg NS1 respectively. Mice blood samples were collected before and after three-day treatment for measurement of zonulin level. The fresh liver was weighted directly and were then used for immunostaining.</p><p><strong>Results: </strong>The C group had lower wet liver weight compared to the T groups (p=0.001). Increased expression of liver zonulin was found in the T2 group, significant different from the C (p=0.014) and T1 groups (p=0.020). After treatment, serum zonulin levels in the T1 group was higher than that of the T1 group before treatment (p=0.035) but not in control (p=0.753) and T2 groups (p=0.869).</p><p><strong>Conclusion: </strong>Administration of 50 μg NS 1 increases wet liver weight and zonulin expression in hepatocytes, but did not increase serum zonulin levels in ddY mice.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 2","pages":"311-317"},"PeriodicalIF":1.4,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/42/85/IJM-15-311.PMC10183082.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9488241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}