{"title":"传统中草药水提物及其组分对HSV-1病毒UL46和US6基因表达水平的影响","authors":"Emad Asgari, Zohreh Sharifi, Farahnaz Bineshian, Mahsa Zamanian","doi":"10.18502/ijm.v16i6.17256","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>Recently, the anti-herpetic activities of different plant species have been investigated. This study evaluated the effects of <i>Teucrium stocksianum</i> aqueous extract on the HSV-1 virus-infected Vero cell.</p><p><strong>Materials and methods: </strong>The IC<sub>50</sub> of the aqueous extract was obtained by the maceration of the plant in boiling water and has been measured with the MTT method, also the q-PCR was used to study viral gene expression reduction.</p><p><strong>Results: </strong>Results of the MTT test indicated that the highest percentage of metabolic activity was observed in the 75 μg/ml concentration of <i>Teucrium stocksianum</i>'s aqueous extracts (IC<sub>50</sub>=45.5μg/ml). Time intervals of 24 and 48 hours after viral infection revealed that the cell viability is reduced by the viral infection time (MOI=0.1), log 10<sup>-3</sup>, p <0.001). Furthermore, the plant's aqueous extract concentration almost avoids cell viability reduction. Through Q-PCR results; the reduction of viral proliferation revealed that the low expression of genes UL46 and US6 were significant in the presence of different treatments utilized in the experiment.</p><p><strong>Conclusion: </strong><i>T. stocksianum</i>, has an anti-viral property and may be considered as a remedy for anti-HSV-1 agents.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 6","pages":"780-785"},"PeriodicalIF":1.3000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682553/pdf/","citationCount":"0","resultStr":"{\"title\":\"Efficacy of traditional herb aqua extract of <i>Teucrium stocksianum</i> and its fractions against HSV-1 virus expression levels of genes (UL46 and US6).\",\"authors\":\"Emad Asgari, Zohreh Sharifi, Farahnaz Bineshian, Mahsa Zamanian\",\"doi\":\"10.18502/ijm.v16i6.17256\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>Recently, the anti-herpetic activities of different plant species have been investigated. This study evaluated the effects of <i>Teucrium stocksianum</i> aqueous extract on the HSV-1 virus-infected Vero cell.</p><p><strong>Materials and methods: </strong>The IC<sub>50</sub> of the aqueous extract was obtained by the maceration of the plant in boiling water and has been measured with the MTT method, also the q-PCR was used to study viral gene expression reduction.</p><p><strong>Results: </strong>Results of the MTT test indicated that the highest percentage of metabolic activity was observed in the 75 μg/ml concentration of <i>Teucrium stocksianum</i>'s aqueous extracts (IC<sub>50</sub>=45.5μg/ml). Time intervals of 24 and 48 hours after viral infection revealed that the cell viability is reduced by the viral infection time (MOI=0.1), log 10<sup>-3</sup>, p <0.001). Furthermore, the plant's aqueous extract concentration almost avoids cell viability reduction. Through Q-PCR results; the reduction of viral proliferation revealed that the low expression of genes UL46 and US6 were significant in the presence of different treatments utilized in the experiment.</p><p><strong>Conclusion: </strong><i>T. stocksianum</i>, has an anti-viral property and may be considered as a remedy for anti-HSV-1 agents.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"16 6\",\"pages\":\"780-785\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682553/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v16i6.17256\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v16i6.17256","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Efficacy of traditional herb aqua extract of Teucrium stocksianum and its fractions against HSV-1 virus expression levels of genes (UL46 and US6).
Background and objectives: Recently, the anti-herpetic activities of different plant species have been investigated. This study evaluated the effects of Teucrium stocksianum aqueous extract on the HSV-1 virus-infected Vero cell.
Materials and methods: The IC50 of the aqueous extract was obtained by the maceration of the plant in boiling water and has been measured with the MTT method, also the q-PCR was used to study viral gene expression reduction.
Results: Results of the MTT test indicated that the highest percentage of metabolic activity was observed in the 75 μg/ml concentration of Teucrium stocksianum's aqueous extracts (IC50=45.5μg/ml). Time intervals of 24 and 48 hours after viral infection revealed that the cell viability is reduced by the viral infection time (MOI=0.1), log 10-3, p <0.001). Furthermore, the plant's aqueous extract concentration almost avoids cell viability reduction. Through Q-PCR results; the reduction of viral proliferation revealed that the low expression of genes UL46 and US6 were significant in the presence of different treatments utilized in the experiment.
Conclusion: T. stocksianum, has an anti-viral property and may be considered as a remedy for anti-HSV-1 agents.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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