Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara.

IF 1.7 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI:10.18502/ijm.v17i1.17812

Inès Quadri, Imene Ikram Hassani, Hocine Hacène

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引用次数: 0

Abstract

Background and objectives: Halophilic archaea commonly produce antimicrobial peptides (halocins), but only a few studies have been conducted on these molecules. This study explores the antimicrobial potential of two strains belonging to Halopiger and Natrialba genera, isolated from hypersaline environments in the Algerian Sahara. Antimicrobial compounds produced by these genera have rarely been studied before.

Materials and methods: The antimicrobial activity of the strains was evaluated, along with the effects of UV radiation and culture conditions on growth and compound production. Stability assays and the effects of extracted compounds on target cells and peripheral blood mononuclear cells (PBMC) were assessed.

Results: The strains exhibited high anti-archaeal activities and cross-domain interactions. Producing extracellular compounds associated with halocin, in the cell-free supernatant (CFS). These compounds remained stable at different temperatures (4°C, 60°C, 80°C, and 100°C) and different pH ranges (4-10 and 5-11), with antimicrobial profiles changed in response to UV light. The active compounds resembled known halocins but displayed unique features suggesting the discovery of new halocins. Additionally, Natrialba extracts showed significant activity against PBMC.

Conclusion: This investigation confirms that Algerian saline soils are a promising source of interesting antimicrobial compounds.

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查看原文本刊更多论文

探索从阿尔及利亚撒哈拉分离的Halopiger属和Natrialba属两种盐古菌的抗菌潜力。

背景和目的：嗜盐古细菌通常产生抗菌肽（halocins），但对这些分子的研究很少。本研究探索了从阿尔及利亚撒哈拉高盐环境中分离出来的两种属于Halopiger和Natrialba属的菌株的抗菌潜力。这些属产生的抗菌化合物以前很少被研究过。材料与方法：研究了菌株的抑菌活性，以及紫外辐射和培养条件对其生长和合成的影响。稳定性试验及提取化合物对靶细胞和外周血单核细胞（PBMC）的影响。结果：菌株具有较高的抗古细菌活性和跨域相互作用。在无细胞上清（CFS）中产生与halocin相关的细胞外化合物。这些化合物在不同温度（4°C、60°C、80°C和100°C）和不同pH范围（4-10和5-11）下保持稳定，抗菌特性随紫外光的变化而变化。活性化合物类似于已知的盐碱素，但显示出独特的特征，表明发现了新的盐碱素。此外，Natrialba提取物对PBMC具有显著的活性。结论：本研究证实阿尔及利亚盐碱地是一个有前景的抗菌化合物来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.