Iranian Journal of Microbiology最新文献_第9页

Investigation of the effect of dimethyl sulfoxide on growth and biofilm formation of Pseudomonas aeruginosa. 二甲基亚砜对铜绿假单胞菌生长及生物膜形成影响的研究。

IF 1.7

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20362

Hafez Al-Momani, Safaa Mashal, Hadeel AlGhawrie

{"title":"Investigation of the effect of dimethyl sulfoxide on growth and biofilm formation of Pseudomonas aeruginosa.","authors":"Hafez Al-Momani, Safaa Mashal, Hadeel AlGhawrie","doi":"10.18502/ijm.v17i6.20362","DOIUrl":"10.18502/ijm.v17i6.20362","url":null,"abstract":"Background and objectives: The antimicrobial resistance of Pseudomonas aeruginosa bacteria limits the spectrum of effective antibiotics. Considerable focus has been placed on the identification of more contemporary and cost-effective antimicrobial drugs. In this study, the antibacterial properties of a commonly used solvent, dimethyl sulfoxide (DMSO), against P. aeruginosa were investigated.Materials and methods: The microtiter broth dilution technique was employed to establish the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of DMSO. The solvent's impact on bacterial growth, biofilm formation and eradication was assessed. A quantitative polymerase chain reaction (qPCR) was carried out to assess the effect of varying DMSO concentrations ranging from 1% to 8% (v/v) on quorum sensing gene expression.Results: All P. aeruginosa strains exhibited a DMSO MIC of 25% v/v and MBC of 50% v/v. DMSO caused significant growth inhibition and suppression of biofilm formation in all P. aeruginosa strains at sub-inhibitory concentrations, i.e. 1%-8% v/v. At these concentrations, the samples showed a reduction in biomass and reduced metabolic activity. These effects were concentration-dependent. A DMSO strength of 8% v/v was associated with a statistically significant downregulation of most of the quorum sensing genes; at a DMSO titer of 1% v/v, this effect was modest with only a few genes being significantly affected.Conclusion: DMSO is a potential therapeutic agent against P. aeruginosa as it has been demonstrated that it exhibits antimicrobial characteristics. Moreover, the impact of DMSO on bacterial growth and biofilm formation complicates its use as a solvent in biologic and clinical research.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"942-953"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimation of second line anti-tubercular drug susceptibility to Mycobacterium tuberculosis in clinical isolates. 临床分离株对结核分枝杆菌二线抗结核药物敏感性评价。

IF 1.7

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20361

Harisree Sudersanan, Nikhilesh Menon Ravikumar, Baijayanti Mishra

{"title":"Estimation of second line anti-tubercular drug susceptibility to Mycobacterium tuberculosis in clinical isolates.","authors":"Harisree Sudersanan, Nikhilesh Menon Ravikumar, Baijayanti Mishra","doi":"10.18502/ijm.v17i6.20361","DOIUrl":"10.18502/ijm.v17i6.20361","url":null,"abstract":"Background and objectives: Tuberculosis (TB) is an infectious disease that is among the most common in the world and is a leading cause of high mortality and morbidity. In India, there is very limited data on second line drug susceptibility testing. The aim of the study was to find out the prevalence of Multi Drug Resistant (MDR) isolates among Mycobacterium tuberculosis (MTB) complex strains and to assess the sensitivity pattern to four commonly used 2nd line anti-tubercular drugs irrespective of their MDR status.Materials and methods: A 61 culture-positive strains of the tuberculosis complex (smear positive or negative) in Mycobacterium Growth Indicator Tube (MGIT) and Lowenstein Jensen (LJ) from various clinical samples were included. We performed MGIT 1st and 2nd line susceptibility testing for tuberculosis.Results: Among the 61 isolates, 12 (19.6%) were multi drug resistant. Capreomycin resistance was observed in 17 (27.8%) isolates, kanamycin resistance in 30 (49.1%), ofloxacin resistance in 5 (8.1%), and ethionamide resistance in 6 (9.8%) isolates. Resistance to kanamycin and ethionamide was more common among patients with multi drug resistant tuberculosis (MDR-TB) than among those with non-MDR-TB.Conclusion: The MGIT system has surpassed solid culture and is an excellent method for performing culture and drug sensitivity testing for tuberculosis. However, its use remains limited by economic and logistical challenges. The high prevalence of aminoglycoside resistance suggests the need to preserve these drugs for treating patients with MDR-TB.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"936-941"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tight junctions expression is affected by active, inactive, and derivatives of Akkermansia muciniphila. 紧密连接的表达受活性、非活性和衍生物的影响。

IF 1.7

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20355

Farinaz Ghaderi, Zahra Hajebrahimi, Abolfazl Fateh, Fattah Sotoodehnejadnematalahi, Sara Ahmadi Badi, Somayeh Vaezijoze, Seyed Davar Siadat

{"title":"Tight junctions expression is affected by active, inactive, and derivatives of Akkermansia muciniphila.","authors":"Farinaz Ghaderi, Zahra Hajebrahimi, Abolfazl Fateh, Fattah Sotoodehnejadnematalahi, Sara Ahmadi Badi, Somayeh Vaezijoze, Seyed Davar Siadat","doi":"10.18502/ijm.v17i6.20355","DOIUrl":"10.18502/ijm.v17i6.20355","url":null,"abstract":"Background and objectives: Tight junctions (TJs) in the gastrointestinal tract are comprised of various junctional proteins including Occludin and Zonula Occludens (ZO-1) that have a critical role in epithelial barrier function. Gut microbiota and their derived metabolites can maintain and regulate gut epithelial barrier integrity.Materials and methods: In the present study, the effects of active, heat-inactivated, cell-free supernatant, and outer membrane vesicles (OMVs) of Akkermansia muciniphila were evaluated on the expression of occludin and ZO-1 genes in Caco-2 cell line by quantitative real-time PCR.Results: Data have shown that both forms of the active (metabolically active, growing, and dividing state), and heat inactivated (by exposure to 56°C for 20 minutes) forms of the bacteria and the cell-free supernatant could affect the expression of occludin and ZO-1 genes (P < 0.05). OMVs significantly increased the expression of the occludin gene but had no effects on the expression of ZO-1.Conclusion: Akkermansia muciniphila and its derived metabolites might have the potential to be used in the pharmaceutical and medicinal fields as probiotic, paraprobiotic and postbiotic agents to prevent metabolic and inflammatory diseases; Although, further research is needed to understand their interactions within the complex gut microbiome and to evaluate potential side effects or risks associated with their use.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"885-892"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The occurrences of E. coli strains with multi-drug resistance profiles and virulence genes from poultry slaughterhouse waste in Abidjan (côte d'ivoire). 阿比让（côte科特迪瓦）家禽屠宰场废物中出现具有多重耐药谱和毒力基因的大肠杆菌菌株。

IF 1.9

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20367

Ainyakou-Sanga Aiza Monique, Goualie Gblossi Bernadette, Kipre Christ Romuald, Kra Kouassi Daniel, Sylla Aboubakar, Yavo Konan Albert, Kakou-Ngazoa Solange, Karou Germain

{"title":"The occurrences of E. coli strains with multi-drug resistance profiles and virulence genes from poultry slaughterhouse waste in Abidjan (côte d'ivoire).","authors":"Ainyakou-Sanga Aiza Monique, Goualie Gblossi Bernadette, Kipre Christ Romuald, Kra Kouassi Daniel, Sylla Aboubakar, Yavo Konan Albert, Kakou-Ngazoa Solange, Karou Germain","doi":"10.18502/ijm.v17i6.20367","DOIUrl":"10.18502/ijm.v17i6.20367","url":null,"abstract":"Background and objectives: Poultry production generates huge quantities of waste, mainly from slaughterhouses, which can be major reservoirs of pathogenic microorganisms. Escherichia coli is of particular concern due to its ability to acquire antibiotic resistance and virulence factors. This study aimed to characterise E. coli contamination in poultry slaughterhouse waste from ten municipalities in the district of Abidjan (Côte d'Ivoire).Materials and methods: E. coli strains were isolated from poultry slaughterhouse waste and identified using morphological and biochemical methods. Antibiotic susceptibility was assessed by the disk diffusion method, and virulence genes, including eae and stx1, were detected using a duplex PCR assay.Results: Between January and April 2023, waste samples were collected, and E. coli strains were isolated and identified. Of 90 isolates, high resistance rates were observed against β-lactams (88.88%), aminoglycosides (77%), and fluoroquinolones (88.87%). MDR was detected in 11.11% of isolates, while 20% produced ESBL. The eae and stx1 genes were detected in 14.47 and 6.57% of isolates, respectively.Conclusion: These results highlight significant antimicrobial resistance and virulence potential in E. coli from poultry slaughterhouse waste, underscoring the need to improve management strategies.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"991-997"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of gut microbiota composition in the fecal samples from type 2 diabetes mellitus patients and healthy individuals: a case control study. 2型糖尿病患者和健康人粪便中肠道菌群组成的比较分析：病例对照研究。

IF 1.7

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20354

Elmira Saiedi, Reza Shapouri, Fakhri Haghi, Habib Zeighami

{"title":"Comparative analysis of gut microbiota composition in the fecal samples from type 2 diabetes mellitus patients and healthy individuals: a case control study.","authors":"Elmira Saiedi, Reza Shapouri, Fakhri Haghi, Habib Zeighami","doi":"10.18502/ijm.v17i6.20354","DOIUrl":"10.18502/ijm.v17i6.20354","url":null,"abstract":"Background and objectives: Insulin resistance and elevated blood glucose levels are the hallmarks of Type 2 Diabetes Mellitus (T2DM), a chronic metabolic condition. Emerging research suggests that gut microbiota may play a causal role in T2DM. This study compares T2DM patients' gut microbiota to healthy controls, focusing on Lactobacillus, Bifidobacterium, Akkermansia muciniphila, Prevotella, Bacteroidetes, and Firmicutes.Materials and methods: This case-control research involved 50 T2DM patients and 50 healthy controls, aged 39-75. Quantitative real-time PCR (qPCR) employing 16S rRNA gene primers was used to detect and quantify bacterial diversity in fecal samples. Statistical analyses were performed to compare the microbiota composition between groups.Results: The gut microbiome of patients with Type 2 Diabetes Mellitus differed significantly from that of healthy controls. In T2DM patients, Lactobacillus spp. and the Firmicutes phylum had higher relative fold differences, while A. muciniphila had lower abundance. No substantial alterations were seen in Bifidobacterium spp., Prevotella, or Bacteroidetes. T2DM patients had more Lactobacillus spp. and Firmicutes and less A. muciniphila in their gut microbiome.Conclusion: While gut microbiota is linked to T2DM, this study analyzes the bacterial composition to identify taxa that change significantly. Further research is essential to unravel the complex relationships between gut microbiota and T2DM pathogenesis, particularly through species-level analysis and genomic studies to identify the primary associated clades.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"875-884"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary investigation of changes in pathogen presence in the vaginal microbiome in association with age. 阴道微生物组中病原体存在变化与年龄相关的初步调查。

IF 1.7

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20357

Subha Maneesha, Borawake Arman, Dubli Kirti, Balasundaram Preethi, Chaudhari Rinku, Jayaprakash Teenus, Kapoor Raman, Singh Raja, Kapoor Anmol, Borkar-Tripathi Minal

{"title":"Preliminary investigation of changes in pathogen presence in the vaginal microbiome in association with age.","authors":"Subha Maneesha, Borawake Arman, Dubli Kirti, Balasundaram Preethi, Chaudhari Rinku, Jayaprakash Teenus, Kapoor Raman, Singh Raja, Kapoor Anmol, Borkar-Tripathi Minal","doi":"10.18502/ijm.v17i6.20357","DOIUrl":"10.18502/ijm.v17i6.20357","url":null,"abstract":"Background and objectives: The vaginal microbiome represents a dynamic ecosystem that undergoes significant transformations throughout a woman's lifespan, influenced by hormonal fluctuations and physiological changes. Interpreting pathogen distribution and developing suitable therapeutic care techniques for women's reproductive health depends on an understanding of these age-related patterns. This study aims to thoroughly describe age-related changes in the makeup of the vaginal microbiome and the distribution of pathogenic species.Materials and methods: Vaginal swab samples were collected from 29 subjects, categorized into different age groups (A: 15-30 years, B: 31-40 years, C: 41-50 years, and D: 51-60 years old females). Microbiome DNA was extracted from the collected vaginal swabs and shotgun next generation sequencing was performed. Post-sequencing, data was analysed using in-house pipeline followed by statistical analysis using R programming.Results: The results showed that microbial diversity varied significantly with age. Group C displayed the most severe pathogenic burden; Group A had the highest overall species diversity with 350 bacterial species. Group D displayed the greatest overall relative abundance levels of microorganisms, primarily due to Lactobacillus rhamnosus dominance.Conclusion: This study shows that the composition of the vaginal microbiome changes fundamentally over the course of a woman's life, with each stage bringing with it its own set of microbial signatures, pathogenic risks, and therapeutic prospects.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"901-911"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human milk as a source of next-generation probiotics: quantifying Akkermansia muciniphila and microbial contamination risks in donor milk. 人乳作为下一代益生菌的来源：定量测定嗜粘阿克曼氏菌和供体乳中的微生物污染风险。

IF 1.7

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20356

Sima Taheri, Morteza Khomeiri, Hesamaddin Shirzad Aski, Hamid Bahador Ghoddusi, Seid Mahdi Jafari, Ezzat Allah Ghaemi

{"title":"Human milk as a source of next-generation probiotics: quantifying Akkermansia muciniphila and microbial contamination risks in donor milk.","authors":"Sima Taheri, Morteza Khomeiri, Hesamaddin Shirzad Aski, Hamid Bahador Ghoddusi, Seid Mahdi Jafari, Ezzat Allah Ghaemi","doi":"10.18502/ijm.v17i6.20356","DOIUrl":"10.18502/ijm.v17i6.20356","url":null,"abstract":"Background and objectives: Human milk provides nutrients, prebiotics, and probiotics that support infants' physical and mental growth. Human milk microbiota, as a potential source of probiotics and an indicator of the safety of donor milk, is of great importance. Akkermansia muciniphila, a core member of next-generation probiotics (NGPs), with the ability to degrade human milk oligosaccharides (HMOs), may be present in human milk. This study was carried out to assess the total bacterial count and presence of A. muciniphila in raw freshly expressed mothers' milk and pasteurized donor milk from human milk banks (HMBs).Materials and methods: 15 raw and 20 pasteurized milk samples were collected and analyzed using a real-time PCR technique with specific primers for A. muciniphila and universal 16S rRNA bacterial primers.Results: Results showed that the average total bacterial count was 4.95 log CFU/ml, which is lower than the normal range reported for human milk. Samples with bacterial count over the standard range were related to the HMBs. Prevalence of A. muciniphila in human milk was 35% and was higher in raw milk samples than in pasteurized samples.Conclusion: In conclusion, raw human milk can serve as a potential source for A. muciniphila isolation as a candidate for NGPs.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"893-900"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of inflammatory scoring tools with spirometry indices in COVID-19 patients: a single center cohort study. COVID-19患者炎症评分工具与肺量测定指标的关联：一项单中心队列研究

IF 1.7

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20375

Mohsen Farrokhpour, Fahimeh Safarnezhad Tameshkel, Niloufar Sadat Kalaki, Azra Asghari Marzidareh, Aliarash Anoushirvani, Neda Rahimian, Mohammad Hadi Karbalaie Niya

{"title":"Association of inflammatory scoring tools with spirometry indices in COVID-19 patients: a single center cohort study.","authors":"Mohsen Farrokhpour, Fahimeh Safarnezhad Tameshkel, Niloufar Sadat Kalaki, Azra Asghari Marzidareh, Aliarash Anoushirvani, Neda Rahimian, Mohammad Hadi Karbalaie Niya","doi":"10.18502/ijm.v17i6.20375","DOIUrl":"10.18502/ijm.v17i6.20375","url":null,"abstract":"Background and objectives: Patients with COVID-19 have spirometry parameters linked to various biological markers, including CRP, NLR, MPV, RDW, and APACHE II score. The objective of this study was to investigate the association of inflammatory scoring tools with spirometry indices in a three-month follow-up of COVID-19 patients.Materials and methods: Spirometry records of 369 COVID-19 cases with complications were analyzed at baseline and three months after discharge. Generalized linear models and logistic regression analysis were performed to compare the variables using SPSS version 25 software.Results: The baseline NLR was 3.20 (95% CI: 2.96, 3.43); PCT was 0.26 (95% CI: 0.25, 0.27); and MPV was 7.23 (95% CI: 7.10, 7.35). We found that the effects of NLR, CRP, and APACHE II score on the respiratory indices FEV1 and FEV1/FVC three months after discharge had an inverse relationship. Patients with asthma had significantly lower FEV1 and FEV1/FVC values, and the level of FVC did not have any significant difference between people with asthma and COPD patients.Conclusion: CRP, NLR, and APACHE II score are among the main factors that are directly related to respiratory indices and they are considered to be appropriate indicators of prognosis for these conditions in COVID-19 patients.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"1042-1048"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular identification and diagnostic challenges of Kodamaea ohmeri fungemia in a neonate - first report from Pakistan. 巴基斯坦首次报道新生儿奥氏小檗真菌病的分子鉴定和诊断挑战。

IF 1.7

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20369

Humaira Farooq, Summiya Nizamuddin, Romena Qazi, Nasrullah Malik, Faisal Sultan

{"title":"Molecular identification and diagnostic challenges of Kodamaea ohmeri fungemia in a neonate - first report from Pakistan.","authors":"Humaira Farooq, Summiya Nizamuddin, Romena Qazi, Nasrullah Malik, Faisal Sultan","doi":"10.18502/ijm.v17i6.20369","DOIUrl":"10.18502/ijm.v17i6.20369","url":null,"abstract":"Background and objectives: Kodamaea ohmeri is an uncommon opportunistic yeast increasingly recognized as a cause of invasive infections, particularly in immunocompromised individuals and neonates. We report the first case of Kodamaea ohmeri fungemia in a neonate from Pakistan, highlighting the diagnostic challenges posed by its close resemblance to more common Candida species.Materials and methods: A one-month-old female neonate with suspected fungal sepsis yielded a yeast isolate. Initial identification was performed using API 20C AUX, followed by colony morphology assessment on Sabouraud Dextrose Agar. Molecular investigations included PCR amplification of the ITS1-5.8S rRNA-ITS2 region, restriction fragment length polymorphism (RFLP) with MspI, and definitive Sanger sequencing. Antifungal susceptibility testing was conducted using standard methods.Results: The isolate was initially misidentified as Candida guilliermondii by API 20C AUX. Colony characteristics suggested an unusual yeast, prompting molecular analysis. PCR produced a ~400 bp amplicon, and RFLP yielded an undigested band, initially suggestive of Candida auris or Candida haemulonii. Sanger sequencing confirmed the organism as K. ohmeri with 99.8% identity. Antifungal testing showed low minimum inhibitory concentrations (MICs) for echinocandins and azoles, while fluconazole demonstrated a higher MIC.Conclusion: This case emphasizes the limitations of conventional methods in identifying rare fungal pathogens, the critical role of molecular confirmation, and the importance of antifungal stewardship in guiding treatment. Reporting such cases contributes to global awareness, surveillance, and improved management of emerging yeast infections.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"1005-1009"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of supplementation on biofilms and antibiotic efficacy against Pseudomonas aeruginosa. 对生物膜的影响及对铜绿假单胞菌的抗菌效果。

IF 1.9

Iranian Journal of Microbiology Pub Date : 2025-12-01 DOI: 10.18502/ijm.v17i6.20353

Mana Salehibarmi, Maryam Siroosi

{"title":"Effect of supplementation on biofilms and antibiotic efficacy against Pseudomonas aeruginosa.","authors":"Mana Salehibarmi, Maryam Siroosi","doi":"10.18502/ijm.v17i6.20353","DOIUrl":"10.18502/ijm.v17i6.20353","url":null,"abstract":"Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, is one of the leading causes of nosocomial infections, particularly in vulnerable patients. However, treating these infections is challenging due to its various antibiotic resistance mechanisms and biofilm formation ability. As traditional antibiotic development struggles to keep pace with evolving resistance, this review explores a promising alternative strategy which is enhancing existing antibiotic efficacy by combining them with nutritional supplements that modulate P. aeruginosa physiology. Specifically, it focuses on studies investigating the effects of diverse carbon, nitrogen, and iron sources on bacterial response to antibiotics, and the mechanisms underlying observed synergy. To achieve this, published literature on P. aeruginosa metabolism, antibiotic resistance, and nutritional influences was comprehensively analyzed and summarized. The findings highlight specific carbon, nitrogen, and iron sources that can enhance various antibiotic classes against P. aeruginosa. These include supplements capable of disrupting biofilm formation, reducing efflux pump activity, or interfering with other resistance mechanisms, thereby increasing antibiotic susceptibility. The specific mechanisms by which these supplements interact with bacterial physiology and antibiotic action are thoroughly discussed. Ultimately, modulating P. aeruginosa physiology through strategic supplementation alongside existing antibiotics offers a promising approach to overcome drug resistance in this pathogen.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 6","pages":"861-874"},"PeriodicalIF":1.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12777386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0