{"title":"伊朗西南部鲍曼不动杆菌分离株生物膜相关基因与耐药性关系的评价","authors":"Nafiseh Hosseinzadeh Shakib, Zahra Hashemizadeh, Abolfazl Rafati Zomorodi, Reza Khashei, Yeganeh Sadeghi, Abdollah Bazargani","doi":"10.18502/ijm.v17i1.17804","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>Increasing antimicrobial resistance among <i>Acinetobacter baumannii (A. baumannii)</i> strains poses a significant challenge, particularly in intensive care units (ICUs) where these bacteria are common causes of hospital infections. Biofilm production is recognized as a key mechanism contributing to this resistance. This study aims to explore the relationship between biofilm production, the presence of biofilm-associated genes, and antibiotic resistance patterns in <i>A. baumannii</i> isolates obtained from ICU patients.</p><p><strong>Materials and methods: </strong>We collected 100 <i>A. baumannii</i> isolates from ICU patients at Nemazee Hospital in Shiraz, Iran. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method, and biofilm production potential was assessed through the tissue culture plate (TCP) method. Additionally, we investigated eleven biofilm-related genes (<i>ompA, bap, csuE, epsA, bla</i> <i><sub>per-1</sub></i> <i>, bfmS, pgaB, csgA, fimH, ptk,</i> and <i>kpsMII</i>) in all isolates using polymerase chain reaction (PCR). The REP-PCR technique was utilized to analyze the genetic relatedness of the isolates (Fig. 4).</p><p><strong>Results: </strong>All isolates displayed multi-drug resistance, with the highest resistance rates observed against ceftazidime, cefotaxime, and trimethoprim/sulfamethoxazole (100%). Gentamicin and amikacin showed the lowest resistance rates at 70% and 84%, respectively. A total of 98% of the isolates were capable of biofilm production, with 32% categorized as strong biofilm producers. The most frequently detected biofilm-associated genes included <i>csuE</i> (99%), <i>bfmS</i> (98%), <i>ompA</i> (97%), and <i>pgaB</i> (89%).</p><p><strong>Conclusion: </strong>Biofilm production significantly contributes to the prevalence of multi-drug resistant <i>A. baumannii</i> strains. It is essential to implement effective antimicrobial stewardship and develop innovative anti-biofilm strategies to address this global health issue.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"80-91"},"PeriodicalIF":1.7000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049743/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the relatedness between the biofilm-associated genes and antimicrobial resistance among <i>Acinetobacter baumannii</i> isolates in the southwest Iran.\",\"authors\":\"Nafiseh Hosseinzadeh Shakib, Zahra Hashemizadeh, Abolfazl Rafati Zomorodi, Reza Khashei, Yeganeh Sadeghi, Abdollah Bazargani\",\"doi\":\"10.18502/ijm.v17i1.17804\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>Increasing antimicrobial resistance among <i>Acinetobacter baumannii (A. baumannii)</i> strains poses a significant challenge, particularly in intensive care units (ICUs) where these bacteria are common causes of hospital infections. Biofilm production is recognized as a key mechanism contributing to this resistance. This study aims to explore the relationship between biofilm production, the presence of biofilm-associated genes, and antibiotic resistance patterns in <i>A. baumannii</i> isolates obtained from ICU patients.</p><p><strong>Materials and methods: </strong>We collected 100 <i>A. baumannii</i> isolates from ICU patients at Nemazee Hospital in Shiraz, Iran. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method, and biofilm production potential was assessed through the tissue culture plate (TCP) method. Additionally, we investigated eleven biofilm-related genes (<i>ompA, bap, csuE, epsA, bla</i> <i><sub>per-1</sub></i> <i>, bfmS, pgaB, csgA, fimH, ptk,</i> and <i>kpsMII</i>) in all isolates using polymerase chain reaction (PCR). The REP-PCR technique was utilized to analyze the genetic relatedness of the isolates (Fig. 4).</p><p><strong>Results: </strong>All isolates displayed multi-drug resistance, with the highest resistance rates observed against ceftazidime, cefotaxime, and trimethoprim/sulfamethoxazole (100%). Gentamicin and amikacin showed the lowest resistance rates at 70% and 84%, respectively. A total of 98% of the isolates were capable of biofilm production, with 32% categorized as strong biofilm producers. The most frequently detected biofilm-associated genes included <i>csuE</i> (99%), <i>bfmS</i> (98%), <i>ompA</i> (97%), and <i>pgaB</i> (89%).</p><p><strong>Conclusion: </strong>Biofilm production significantly contributes to the prevalence of multi-drug resistant <i>A. baumannii</i> strains. It is essential to implement effective antimicrobial stewardship and develop innovative anti-biofilm strategies to address this global health issue.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"17 1\",\"pages\":\"80-91\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049743/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v17i1.17804\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v17i1.17804","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Evaluation of the relatedness between the biofilm-associated genes and antimicrobial resistance among Acinetobacter baumannii isolates in the southwest Iran.
Background and objectives: Increasing antimicrobial resistance among Acinetobacter baumannii (A. baumannii) strains poses a significant challenge, particularly in intensive care units (ICUs) where these bacteria are common causes of hospital infections. Biofilm production is recognized as a key mechanism contributing to this resistance. This study aims to explore the relationship between biofilm production, the presence of biofilm-associated genes, and antibiotic resistance patterns in A. baumannii isolates obtained from ICU patients.
Materials and methods: We collected 100 A. baumannii isolates from ICU patients at Nemazee Hospital in Shiraz, Iran. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method, and biofilm production potential was assessed through the tissue culture plate (TCP) method. Additionally, we investigated eleven biofilm-related genes (ompA, bap, csuE, epsA, blaper-1, bfmS, pgaB, csgA, fimH, ptk, and kpsMII) in all isolates using polymerase chain reaction (PCR). The REP-PCR technique was utilized to analyze the genetic relatedness of the isolates (Fig. 4).
Results: All isolates displayed multi-drug resistance, with the highest resistance rates observed against ceftazidime, cefotaxime, and trimethoprim/sulfamethoxazole (100%). Gentamicin and amikacin showed the lowest resistance rates at 70% and 84%, respectively. A total of 98% of the isolates were capable of biofilm production, with 32% categorized as strong biofilm producers. The most frequently detected biofilm-associated genes included csuE (99%), bfmS (98%), ompA (97%), and pgaB (89%).
Conclusion: Biofilm production significantly contributes to the prevalence of multi-drug resistant A. baumannii strains. It is essential to implement effective antimicrobial stewardship and develop innovative anti-biofilm strategies to address this global health issue.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
