Evaluation of the relatedness between the biofilm-associated genes and antimicrobial resistance among Acinetobacter baumannii isolates in the southwest Iran.

IF 1.7 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI:10.18502/ijm.v17i1.17804

Nafiseh Hosseinzadeh Shakib, Zahra Hashemizadeh, Abolfazl Rafati Zomorodi, Reza Khashei, Yeganeh Sadeghi, Abdollah Bazargani

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Abstract

Background and objectives: Increasing antimicrobial resistance among Acinetobacter baumannii (A. baumannii) strains poses a significant challenge, particularly in intensive care units (ICUs) where these bacteria are common causes of hospital infections. Biofilm production is recognized as a key mechanism contributing to this resistance. This study aims to explore the relationship between biofilm production, the presence of biofilm-associated genes, and antibiotic resistance patterns in A. baumannii isolates obtained from ICU patients.

Materials and methods: We collected 100 A. baumannii isolates from ICU patients at Nemazee Hospital in Shiraz, Iran. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method, and biofilm production potential was assessed through the tissue culture plate (TCP) method. Additionally, we investigated eleven biofilm-related genes (ompA, bap, csuE, epsA, bla _per-1 , bfmS, pgaB, csgA, fimH, ptk, and kpsMII) in all isolates using polymerase chain reaction (PCR). The REP-PCR technique was utilized to analyze the genetic relatedness of the isolates (Fig. 4).

Results: All isolates displayed multi-drug resistance, with the highest resistance rates observed against ceftazidime, cefotaxime, and trimethoprim/sulfamethoxazole (100%). Gentamicin and amikacin showed the lowest resistance rates at 70% and 84%, respectively. A total of 98% of the isolates were capable of biofilm production, with 32% categorized as strong biofilm producers. The most frequently detected biofilm-associated genes included csuE (99%), bfmS (98%), ompA (97%), and pgaB (89%).

Conclusion: Biofilm production significantly contributes to the prevalence of multi-drug resistant A. baumannii strains. It is essential to implement effective antimicrobial stewardship and develop innovative anti-biofilm strategies to address this global health issue.

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伊朗西南部鲍曼不动杆菌分离株生物膜相关基因与耐药性关系的评价

背景和目的：鲍曼不动杆菌（鲍曼不动杆菌）菌株日益增加的抗菌素耐药性构成了重大挑战，特别是在重症监护病房（icu），这些细菌是医院感染的常见原因。生物膜的产生被认为是促成这种耐药性的关键机制。本研究旨在探讨从ICU患者中获得的鲍曼不动杆菌分离株的生物膜生成、生物膜相关基因的存在和抗生素耐药性模式之间的关系。材料与方法：从伊朗设拉子Nemazee医院ICU患者中分离出100株鲍曼不动杆菌。采用Kirby-Bauer纸片扩散法进行抗菌药敏试验（AST），采用组织培养平板法（TCP）评估生物膜生产潜力。此外，我们利用聚合酶链反应（PCR）研究了所有分离株的11个生物膜相关基因（ompA、bap、csuE、epsA、bla per-1、bfmS、pgaB、csgA、fimH、ptk和kpsMII）。利用REP-PCR技术分析分离株的遗传亲缘关系（图4）。结果：所有分离株均显示多重耐药，对头孢他啶、头孢噻肟和甲氧苄啶/磺胺甲恶唑的耐药率最高（100%）。庆大霉素和阿米卡星的耐药率最低，分别为70%和84%。共有98%的分离株能够产生生物膜，其中32%被归类为强生物膜生产者。最常检测到的生物膜相关基因包括csuE（99%）、bfmS（98%）、ompA（97%）和pgaB（89%）。结论：生物膜的产生是多重耐药鲍曼不动杆菌流行的重要因素。必须实施有效的抗微生物药物管理并制定创新的抗生物膜战略，以解决这一全球卫生问题。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.