mecA and PVL genes in methicillin-resistant Staphylococcus aureus from clinical specimens: a cross-sectional hospital based study from Nepal.

IF 1.7 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI:10.18502/ijm.v17i1.17806

Sirjana Adhikari, Supriya Sharma, Sanjib Adhikari, Sanjit Shrestha, Dwij Raj Bhatta

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引用次数: 0

Abstract

Background and objectives: Staphylococcus aureus has increasingly been associated with community and healthcare-associated infections worldwide and contributes to treatment failures due to the emergence of multidrug-resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) strains. We aimed to determine the prevalence and antibiotic susceptibility pattern of MRSA isolated from patients attending a burn center and to detect mecA and PVL genes among MRSA isolates.

Materials and methods: A cross-sectional hospital based study was conducted on 1950 clinical samples collected from hospital inpatients and outpatients of Kirtipur Hospital, which is a burn specialist hospital in Kathmandu, Nepal. Each sample underwent conventional cultural methods for bacterial isolates identification.

Results: Out of 1950 samples, 452 (23.2%) samples showed bacterial growth, of which 109 isolates (24.1%) were identified as Gram positive and 343 (75.9%) as Gram negative bacteria. Among the Gram positive bacteria, 53 (48.62%) were Staphylococcus aureus. Of the total S. aureus isolates, 40 (75.5%) were MRSA and 48 (90.6%) were MDR. Of the 40 MRSA isolates, 29 (72.5%) carried the mecA gene and 3 (7.5%) harbored PVL gene.

Conclusion: The high prevalence of MRSA in a burn unit underscores the need for more rigorous infection control practices that follow standard protocols to reduce MRSA transmission in both individuals and the hospital environment.

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临床标本中耐甲氧西林金黄色葡萄球菌的mecA和PVL基因：尼泊尔一项基于医院的横断面研究

背景和目的：金黄色葡萄球菌在世界范围内越来越多地与社区和卫生保健相关感染相关，并且由于耐多药（MDR）和耐甲氧西林金黄色葡萄球菌（MRSA）菌株的出现导致治疗失败。我们的目的是确定在烧伤中心就诊的患者中分离的MRSA的患病率和抗生素敏感性模式，并检测MRSA分离株中的mecA和PVL基因。材料和方法：对尼泊尔加德满都一家烧伤专科医院Kirtipur医院的住院和门诊患者1950例临床样本进行了横断面医院研究。每个样品都采用常规培养方法进行细菌分离鉴定。结果：1950份样品中有452株（23.2%）细菌生长，其中革兰阳性109株（24.1%），革兰阴性343株（75.9%）。革兰氏阳性菌中，金黄色葡萄球菌53株（48.62%）；其中MRSA 40株（75.5%），MDR 48株（90.6%）。40株MRSA分离株中，29株（72.5%）携带mecA基因，3株（7.5%）携带PVL基因。结论：MRSA在烧伤病房的高流行率强调需要更严格的感染控制实践，遵循标准方案，以减少MRSA在个人和医院环境中的传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.