Iranian Journal of Microbiology最新文献_第8页

Design of ELISA-based diagnostic system for detection of enterohaemorrhagic Escherichia coli. 肠出血性大肠杆菌elisa诊断系统的设计。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI: 10.18502/ijm.v17i2.18388

Mohammad Javad Rezaei, Maryam Eidi, Seyed Ali Mirhosseini, Rouhollah Kazemi, Mohammad Javad Motamedi, Soghra Khani, Jafar Amani

{"title":"Design of ELISA-based diagnostic system for detection of enterohaemorrhagic Escherichia coli.","authors":"Mohammad Javad Rezaei, Maryam Eidi, Seyed Ali Mirhosseini, Rouhollah Kazemi, Mohammad Javad Motamedi, Soghra Khani, Jafar Amani","doi":"10.18502/ijm.v17i2.18388","DOIUrl":"https://doi.org/10.18502/ijm.v17i2.18388","url":null,"abstract":"Background and objectives: Escherichia coli (E. coli) O157:H7 is an intestinal pathogen of humans and animals, which causes serious gastrointestinal, urinary tract infection and hemolytic uremic syndrome. Connecting to the host cell is important in pathogenesis. EspA, Intimin and Tir proteins (EIT) are the most important bacterial features in the process of binding. These antigens can be very useful in detecting these bacteria. The aim of this study was to produce recombinant EspA, Intimin and Tir proteins (rEIT) to detect pathogenic E. coli O157:H7 by means of ELISA method.Materials and methods: The eit recombinant gene was expressed using IPTG in E. coli BL21 (DE3) and evaluated by western blotting. The purified rEIT protein was injected to rabbits and mice subcutaneously. Purified antibody was evaluated using indirect, competitive and sandwich ELISA confirming the precise detection of E. coli O157: H7.Results: Indirect, competitive and sandwich ELISA specifically detected E. coli O157:H7 and each methods had the ability to identify more than 104, 104, 103 bacteria. The specificity of this method was evaluated by Entroheamoragic E. coli, enterotoxygenic E. coli, Klebsiella pneumoniae, Vibrio cholera and Acinetobacter.Conclusion: These methods are the fastest, most accurate and cost effective methods for diagnosis of E. coli O157: H7, comparing to the conventional methods.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"278-286"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of Staphylococcal Enterotoxin B (SEB) toxoid on lung and liver tissue integrity in rats during systemic infection. 葡萄球菌肠毒素B （SEB）类毒素对全身感染大鼠肺和肝组织完整性的保护作用。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI: 10.18502/ijm.v17i2.18396

Dhafer Rasheed Al-Fetly, Atiaf Ghanim Rhyaf, Hala Abbas Naji

{"title":"Protective effects of Staphylococcal Enterotoxin B (SEB) toxoid on lung and liver tissue integrity in rats during systemic infection.","authors":"Dhafer Rasheed Al-Fetly, Atiaf Ghanim Rhyaf, Hala Abbas Naji","doi":"10.18502/ijm.v17i2.18396","DOIUrl":"https://doi.org/10.18502/ijm.v17i2.18396","url":null,"abstract":"Background and objectives: Staphylococcal enterotoxin B (SEB), a potent superantigenic toxin produced by Staphylococcus aureus (S. aureus), plays a crucial role in S. aureus systemic infection. This investigation sought to determine whether immunising animals with SEB toxoid could protect against an experimental acute systemic infection caused by S. aureus.Materials and methods: This study involved three groups of animals: one group was administered with SEB toxoid, and the second group was administered with intramuscular injections of normal saline, after which both were subjected to systemic S. aureus infection. The third group served as the negative control. After two weeks, the outcomes of the experimental systemic infection demonstrated that SEB immunisation significantly shielded organs (lung and liver) from damage in comparison to the control group.Results: Regarding the histopathological analysis of liver and lung tissues, the control group showed minimal alterations, indicating a normal tissue state. Infected individuals exhibited severe pathology, including inflammation, necrosis, and fibrosis. The immunised group displayed a mixed profile with elevated inflammation but lower necrosis and fibrosis. Immunisation mitigated pathological changes induced by infection, fostering a more controlled response.Conclusion: SEB plays an important role in S. aureus pathogenesis and immunisation, and this toxoid might protect against fatal infections of S. aureus.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"220-228"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the ability of Saccharomyces cerevisiae and Lactobacillus plantarum on the reduction of aflatoxin B₁, ochratoxin A, and zearalenone in dough and toast Bread. 研究了酿酒酵母和植物乳杆菌对面团和烤面包中黄曲霉毒素B1、赭曲霉毒素A和玉米赤霉烯酮的还原能力。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI: 10.18502/ijm.v17i2.18393

Alireza Haji Amiri, Leila Nateghi, Nazanin Zand

{"title":"Investigating the ability of Saccharomyces cerevisiae and Lactobacillus plantarum on the reduction of aflatoxin B1, ochratoxin A, and zearalenone in dough and toast Bread.","authors":"Alireza Haji Amiri, Leila Nateghi, Nazanin Zand","doi":"10.18502/ijm.v17i2.18393","DOIUrl":"10.18502/ijm.v17i2.18393","url":null,"abstract":"Background and objectives: Wheat and its derived products are high-risk commodities for aflatoxin contamination. The objective of this study was to investigate the effect of using Saccharomyces cerevisiae, Lactobacillus plantarum, and the dough fermentation and baking periods on reducing aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEA) toxins.Materials and methods: Toast bread flour contaminated with AFB1, OTA and ZEA (10,10 and 400 ng/g) were separately treated with S. cerevisiae and L. plantarum (at a concentration of 108 CFU/g). The reduction of mycotoxins was examined immediately after dough preparation, at the end of fermentation, and after baking.Results: The type of microorganism, fermentation and baking significantly affected the reduction of mycotoxins (AFB1, OTA, and ZEA). After baking, neither AFB1 nor OTA were detected in any of the toast bread samples, with a 100% reduction observed in all treatments. In contrast, the percentage reduction of ZEA after baking compared with immediately after dough preparation ranged from 98.90% to 100%, and the percentage reduction of ZEA at the end of fermentation compared with immediately after dough preparation ranged from 97.80% to 99.57%.Conclusion: The findings of this study suggest that L. plantarum and S. cerevisiae can be used as additives or processing agents to decrease mycotoxins in fermented wheat foods.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"328-341"},"PeriodicalIF":1.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from women with urinary tract infections in Jordan. 约旦尿路感染妇女中分离出的产生扩展谱β -内酰胺酶（ESBL）的大肠杆菌的发病率

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI: 10.18502/ijm.v17i1.17800

Rania Al-Groom

{"title":"Incidence of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from women with urinary tract infections in Jordan.","authors":"Rania Al-Groom","doi":"10.18502/ijm.v17i1.17800","DOIUrl":"https://doi.org/10.18502/ijm.v17i1.17800","url":null,"abstract":"Background and objectives: Urinary tract infections are one of the world's major health problems. In addition, clinical disorders may result from the presence of bacteria or fungi in urine. The aim of this study was to isolate Escherichia coli (E. coli) strains from midstream urine samples, and to determine molecular characterization of encoded Extended Spectrum Beta-Lactamase (ESBL) genes.Materials and methods: Collected urine samples were streaked on MacConkey, blood and EMB agar plates, then identifying E. coli isolates by using antibiotic susceptibility tests. ESBL production was measured using double disc diffusion. Furthermore, uniplex PCR was performed to identify two ESBL genes (bla CTX and bla TEM).Results: Among 412 isolates, 198 (48.1%) were E. coli strains, followed by Staphylococcus saprophyticus, Klebsiella sp., Serratia sp., Enterococci sp. and Proteus sp. with frequency of 132 (32.0%), 51 (12.4%), 15 (3.6%), 10 (2.4%), and 6 (1.5%) respectively. Female participants who were between the ages of 40 and 49 years old, married, and pregnant were more likely to develop urinary tract infections (UTIs). E. coli species were present in 189 (95.5) of the recurrent UTIs. Regarding antimicrobial susceptibility testing of E. coli isolates, the highest percentage of resistance and susceptible rates were found for nalidixic acid (75.8%) and gentamicin (64.1%) respectively. Among the E. coli isolates, 25 (12.6%) were ESBL-producers. The bla CTX-M gene was genetically confirmed in 20 (10.1%) of the isolates.Conclusion: E. coli is the most common cause of UTI and ESBL production leads to increased resistance to common antibiotics and complicates treatment strategies.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"41-50"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical components and antibacterial activity of two populations of Senecio vulgaris L. essential oils as traditional medicine plant. 两种药用植物荆芥精油的化学成分及抑菌活性研究。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI: 10.18502/ijm.v17i1.17815

Fatemeh Hajmoradi, Hamed Fathi, Foozieh Moghadami

{"title":"Phytochemical components and antibacterial activity of two populations of Senecio vulgaris L. essential oils as traditional medicine plant.","authors":"Fatemeh Hajmoradi, Hamed Fathi, Foozieh Moghadami","doi":"10.18502/ijm.v17i1.17815","DOIUrl":"https://doi.org/10.18502/ijm.v17i1.17815","url":null,"abstract":"Background and objectives: Senecio vulgaris L., a member of the Asteraceae family, has been widely employed in traditional Iranian herbal practices for centuries. This research seeks to analyze and compare the essential oil compositions and antibacterial characteristics of two distinct populations of S. vulgaris.Materials and methods: Essential oils were obtained from the above-ground parts of these populations through hydrodistillation, and their chemical constituents were examined using gas chromatography-mass spectrometry. The antibacterial effectiveness of the essential oils against both Gram-positive and Gram-negative bacteria was evaluated employing the agar well diffusion technique.Results: Monoterpene hydrocarbons were found to be dominated in both populations, with Humulene epoxide II being the primary constituent, constituting 17.87% in the first population and 21.55% in the second one. The agar-well diffusion method revealed significant antibacterial effects of the S. vulgaris essential oils. The findings indicated that the essential oil displayed heightened activity against Escherichia coli in both populations. Furthermore, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests indicated that Pseudomonas aeruginosa with concentrations of 400 μg/mL for both tests, was the most susceptible bacteria, while Streptococcus pyogenes with MIC = 800 and MBC>800 μg/mL was the most resistant in both populations of S. vulgaris.Conclusion: This research highlights the significance of S. vulgaris as a valuable reservoir of monoterpene-rich oil exhibiting robust antibacterial characteristics, suggesting its potential use in the development of novel and naturally derived therapeutics for bacterial diseases.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"171-179"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive review of preclinical evaluation strategies for COVID-19 vaccine candidates: assessing immunogenicity, toxicology, and safety profiles. COVID-19候选疫苗临床前评估策略的综合综述：评估免疫原性、毒理学和安全性概况

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI: 10.18502/ijm.v17i1.17796

Delaram Doroud, Mojtaba Daneshi, Fatemeh Kazemi-Lomedash, Zohre Eftekhari

{"title":"Comprehensive review of preclinical evaluation strategies for COVID-19 vaccine candidates: assessing immunogenicity, toxicology, and safety profiles.","authors":"Delaram Doroud, Mojtaba Daneshi, Fatemeh Kazemi-Lomedash, Zohre Eftekhari","doi":"10.18502/ijm.v17i1.17796","DOIUrl":"https://doi.org/10.18502/ijm.v17i1.17796","url":null,"abstract":"Following the worldwide spread of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is a vital requirement for safe and effective vaccines against Coronavirus disease 2019 (COVID-19). Therefore, several vaccine-candidate platforms have been designed, tested, and developed. Based on guidelines, preclinical studies are recommended to assess the safety and potency of COVID-19 vaccines in appropriate in vitro and in vivo settings. These studies provide essential information to describe the potential toxic properties of a vaccine and the formulation of vaccine agents during the preclinical trial phase. In toxicology studies, several factors must be considered, such as the appropriate animal species and strains, dosing timetable, mode of administration, time of sampling for biochemistry and antibody evaluation, and necropsy. Pharmacokinetic/ biodistribution studies are not usually required for infectious disease prophylaxis vaccines unless the vaccine contains a novel substance. Evaluating their biodistribution is crucial for newly developed vaccines, such as lipid nanoparticles -messenger RNA (LNP-mRNA), DNA, and Viral vectors in non-replicated (VVnr), or recombinant virus vaccines. The review highlights the importance of preclinical studies in assessing the safety and efficacy of vaccine candidates. This guidance is essential for researchers and manufacturers to design effective vaccines that can progress to clinical trials safely.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"1-18"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns. 脓毒性关节炎中的大肠杆菌：患病率和抗生素敏感性模式。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI: 10.18502/ijm.v17i1.17798

Masroora Ali Khan, Muhammad Kamran Taj, Zahid Khan, Imran Taj, Muhammad Naeem, Saima Azam

{"title":"Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns.","authors":"Masroora Ali Khan, Muhammad Kamran Taj, Zahid Khan, Imran Taj, Muhammad Naeem, Saima Azam","doi":"10.18502/ijm.v17i1.17798","DOIUrl":"https://doi.org/10.18502/ijm.v17i1.17798","url":null,"abstract":"Background and objectives: Septic arthritis (SA) is an orthopedic emergency mainly caused by bacteria. SA due to Escherichia coli (E. coli) is rare with a poor prognosis. This study aimed to assess the occurrence and antibiotic resistance patterns of E. coli in SA patients in Quetta, Balochistan, Pakistan.Materials and methods: A cross-sectional study was conducted from March 2021 to December 2023. 220 samples were collected from SA patients from tertiary care hospitals. Joint aspirates (2ml) and blood (5ml) were analyzed for microbial and hematological examination.Results: There were 5.45% samples positive, and 94.5% negative for E. coli. SA due to E. coli was more common in male (6.2%) than female (4.6%) patients with the knee being the most affected joint (6.3%). E. coli was more common in patients aged 41-60 years (7.7%), lower socioeconomic (6.9%), and illiterate (8.6%) patients. Suspected patients showed a significant increase in the levels of white blood cells (WBC), C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR), notably, these levels were further elevated in E. coli-positive patients. The polymerase chain reaction (PCR) based identification of E. coli showed clear bands of 204bp of the 16S rRNA gene. Sequence analysis using the Basic Local Alignment Search Tool found high similarity with pathogenic E. coli from Egypt and China. The identified E. coli strain showed significant resistance to common antibiotics: amoxicillin, amoxicillin-clavulanate, ceftriaxone, sulfamethoxazole/trimethoprim, gentamicin, tetracycline, and erythromycin.Conclusion: Antibiotic resistance in E. coli from SA patients suggests the need for accurate antibiotic selection to ensure prompt treatment.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"25-31"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of Rhizobium, Bacillus and Arbuscular Mycorrhizal fungi on enhancing cotton growth. 根瘤菌、芽孢杆菌和丛枝菌根真菌对棉花生长的协同效应。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI: 10.18502/ijm.v17i1.17816

Tasleem Sultana, Pavan Kumar Pindi

{"title":"Synergistic effects of Rhizobium, Bacillus and Arbuscular Mycorrhizal fungi on enhancing cotton growth.","authors":"Tasleem Sultana, Pavan Kumar Pindi","doi":"10.18502/ijm.v17i1.17816","DOIUrl":"https://doi.org/10.18502/ijm.v17i1.17816","url":null,"abstract":"Background and objectives: Telangana district is renowned for its prominence in cotton production, a crop vital to the livelihoods of local farmers. For years, synthetic fertilizers have been relied upon to bolster yields, but escalating costs have shifted focus towards biofertilizers as a cost-effective and sustainable alternative.Materials and methods: A microbial consortium comprising Rhizobium sp. PKS, Bacillus sp. PU-7, and Funneliformis mosseae AMF was employed. Microbial identification was performed using 16S rRNA gene sequencing. Biochemical evaluations of consortium-inoculated plants included measurements of protein, sugar, proline, and chlorophyll levels, along with IAA quantification.Results: A consortium of Bacillus sp. PU-7, Rhizobium sp. Pks [NCBI OK663003, NCMR-MCC4960], and Funneliformis mosseae enhanced Mahyco cultivar growth. Treatment increased plant height, fresh and dry weight, and improved biochemical profiles (reduced proline, elevated IAA, protein, chlorophyll, and sugars). Soil field trials were undertaken in four cotton-producing regions of Mahabubnagar region confirmed for efficacy, with deep black soil promoting phytohormone synthesis (IAA-917.66 ± 2.51) and light black soil (IAA-802 ± 2) enhancing plant growth.Conclusion: Given these outcomes, the application of the tested bioinoculants and AMF spores is suggested as an effective strategy to enhance cotton development and yield in the soils of Mahabubnagar, potentially revolutionizing the district's agricultural practices.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"180-193"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro investigations of coelomic fluid of Eisenia fetida: protein analysis, antioxidant activities and antibacterial effects on diabetic wounds' bacteria. 飞天爱森菌体腔液的体外研究：蛋白质分析、抗氧化活性及对糖尿病创面细菌的抗菌作用。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI: 10.18502/ijm.v17i1.17814

Negar Danafar, Marzieh Shokoohi

{"title":"In vitro investigations of coelomic fluid of Eisenia fetida: protein analysis, antioxidant activities and antibacterial effects on diabetic wounds' bacteria.","authors":"Negar Danafar, Marzieh Shokoohi","doi":"10.18502/ijm.v17i1.17814","DOIUrl":"https://doi.org/10.18502/ijm.v17i1.17814","url":null,"abstract":"Background and objectives: Diabetes is a metabolic disorder characterized by elevated glucose levels, leading to complications such as infections and impaired wound healing. Diabetic wounds are prone to bacterial infections, with common pathogens including Staphylococcus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa. Coelomic fluid of Eisenia fetida (CFEF) exhibits antimicrobial properties, making it a potential alternative to traditional antibiotics. This study aims to evaluate the in vitro antibacterial effects of CFEF on diabetic wound pathogens, alongside analyzing its protein content and antioxidant activities.Materials and methods: This study used bacterial strains Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 19659, and Pseudomonas aeruginosa ATCC 27853. CFEF was extracted using warm water and electric shock methods. Protein concentration was determined using the Bradford method, and protein analysis was conducted via Tricine SDS-PAGE. Antioxidant activities were evaluated using DPPH, FRAP, superoxide dismutase, and catalase assays. Antibacterial activities were tested by disc diffusion, MIC, and MBC methods.Results: The study showed that CFEF exhibited significant antibacterial and antioxidant activities against common bacteria found in diabetic wound infections. The warm water shock method yielded superior results compared to the electric shock method.Conclusion: CFEF demonstrates promising antibacterial and antioxidant properties, suggesting its potential as a natural alternative for treating diabetic wound infections. Further research is needed to evaluate its clinical application and safety.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"163-170"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serological epidemiology analysis of Cytomegalovirus infection in pregnant women in Diwaniyah, Iraq. 伊拉克迪瓦尼耶孕妇巨细胞病毒感染的血清学流行病学分析

IF 1.3

Iranian Journal of Microbiology Pub Date : 2025-02-01 DOI: 10.18502/ijm.v17i1.17810

Mohsen Abdul Khadhur Al-Zubaidi, Seyed Masoud Hosseini, Mohsen Abdul Nemah Al-Roudhan, Maather Baqer Hussein Al-Harmooshee

{"title":"Serological epidemiology analysis of Cytomegalovirus infection in pregnant women in Diwaniyah, Iraq.","authors":"Mohsen Abdul Khadhur Al-Zubaidi, Seyed Masoud Hosseini, Mohsen Abdul Nemah Al-Roudhan, Maather Baqer Hussein Al-Harmooshee","doi":"10.18502/ijm.v17i1.17810","DOIUrl":"https://doi.org/10.18502/ijm.v17i1.17810","url":null,"abstract":"Background and objectives: Human cytomegalovirus (HCMV) infection is the most common cause of congenital infection during pregnancy. It is a major concern worldwide with a wide range of clinical outcomes in fetuses and newborns due to HCMV reactivation or reinfection during pregnancy. Primary maternal infection is best diagnosed by examining IgM and IgG antibodies. The current study aimed epidemiology survey of congenital HCMV infection in pregnant women in Diwaniyah.Materials and methods: 600 blood samples were collected from pregnant women, between 18-45 years old, in Diwaniyah Governorate for 12 months, from January to December 2022, in regards to their place of residence (urban or rural). All samples were monitored for both IgG and IgM antibodies against HCMV using rapid test and ELISA.Results: Our findings showed a high positive rate for IgG (95.7%) and (96.2%) and a positive rate for IgM (1.5%) and (1.8%) for rapid test and ELISA, respectively. The highest IgG positive rate was in the age group 26-35 years (43.33%), while the lowest rate (13.0%) was in the age group 36-45 years. The HCMV infection rate in rural and urban areas were (96.48%) and (95.26%), respectively, with no significant differences (P value>0.05). Also, the rate of miscarriages among pregnant women infected with HCMV was 28.83%, and the highest infection rate (30.51%) was recorded in the age group 26-35 years.Conclusion: The prevalence of HCMV infection and its related miscarriage among the studied population is relatively high with the highest rate in the age group of 26-35 years.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"128-136"},"PeriodicalIF":1.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0