{"title":"约旦尿路感染妇女中分离出的产生扩展谱β -内酰胺酶（ESBL）的大肠杆菌的发病率","authors":"Rania Al-Groom","doi":"10.18502/ijm.v17i1.17800","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>Urinary tract infections are one of the world's major health problems. In addition, clinical disorders may result from the presence of bacteria or fungi in urine. The aim of this study was to isolate <i>Escherichia coli (E. coli)</i> strains from midstream urine samples, and to determine molecular characterization of encoded Extended Spectrum Beta-Lactamase (ESBL) genes.</p><p><strong>Materials and methods: </strong>Collected urine samples were streaked on MacConkey, blood and EMB agar plates, then identifying <i>E. coli</i> isolates by using antibiotic susceptibility tests. ESBL production was measured using double disc diffusion. Furthermore, uniplex PCR was performed to identify two ESBL genes (<i>bla</i> <sub>CTX</sub> and <i>bla</i> <sub>TEM</sub>).</p><p><strong>Results: </strong>Among 412 isolates, 198 (48.1%) were <i>E. coli</i> strains, followed by <i>Staphylococcus saprophyticus, Klebsiella</i> sp., <i>Serratia</i> sp., <i>Enterococci</i> sp. and <i>Proteus</i> sp. with frequency of 132 (32.0%), 51 (12.4%), 15 (3.6%), 10 (2.4%), and 6 (1.5%) respectively. Female participants who were between the ages of 40 and 49 years old, married, and pregnant were more likely to develop urinary tract infections (UTIs). <i>E. coli</i> species were present in 189 (95.5) of the recurrent UTIs. Regarding antimicrobial susceptibility testing of <i>E. coli</i> isolates, the highest percentage of resistance and susceptible rates were found for nalidixic acid (75.8%) and gentamicin (64.1%) respectively. Among the <i>E. coli</i> isolates, 25 (12.6%) were ESBL-producers. The <i>bla</i> <sub>CTX-M</sub> gene was genetically confirmed in 20 (10.1%) of the isolates.</p><p><strong>Conclusion: </strong><i>E. coli</i> is the most common cause of UTI and ESBL production leads to increased resistance to common antibiotics and complicates treatment strategies.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 1","pages":"41-50"},"PeriodicalIF":1.7000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049747/pdf/","citationCount":"0","resultStr":"{\"title\":\"Incidence of extended spectrum beta-lactamase (ESBL) producing <i>Escherichia coli</i> isolated from women with urinary tract infections in Jordan.\",\"authors\":\"Rania Al-Groom\",\"doi\":\"10.18502/ijm.v17i1.17800\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>Urinary tract infections are one of the world's major health problems. In addition, clinical disorders may result from the presence of bacteria or fungi in urine. The aim of this study was to isolate <i>Escherichia coli (E. coli)</i> strains from midstream urine samples, and to determine molecular characterization of encoded Extended Spectrum Beta-Lactamase (ESBL) genes.</p><p><strong>Materials and methods: </strong>Collected urine samples were streaked on MacConkey, blood and EMB agar plates, then identifying <i>E. coli</i> isolates by using antibiotic susceptibility tests. ESBL production was measured using double disc diffusion. Furthermore, uniplex PCR was performed to identify two ESBL genes (<i>bla</i> <sub>CTX</sub> and <i>bla</i> <sub>TEM</sub>).</p><p><strong>Results: </strong>Among 412 isolates, 198 (48.1%) were <i>E. coli</i> strains, followed by <i>Staphylococcus saprophyticus, Klebsiella</i> sp., <i>Serratia</i> sp., <i>Enterococci</i> sp. and <i>Proteus</i> sp. with frequency of 132 (32.0%), 51 (12.4%), 15 (3.6%), 10 (2.4%), and 6 (1.5%) respectively. Female participants who were between the ages of 40 and 49 years old, married, and pregnant were more likely to develop urinary tract infections (UTIs). <i>E. coli</i> species were present in 189 (95.5) of the recurrent UTIs. Regarding antimicrobial susceptibility testing of <i>E. coli</i> isolates, the highest percentage of resistance and susceptible rates were found for nalidixic acid (75.8%) and gentamicin (64.1%) respectively. Among the <i>E. coli</i> isolates, 25 (12.6%) were ESBL-producers. The <i>bla</i> <sub>CTX-M</sub> gene was genetically confirmed in 20 (10.1%) of the isolates.</p><p><strong>Conclusion: </strong><i>E. coli</i> is the most common cause of UTI and ESBL production leads to increased resistance to common antibiotics and complicates treatment strategies.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"17 1\",\"pages\":\"41-50\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049747/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v17i1.17800\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v17i1.17800","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Incidence of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from women with urinary tract infections in Jordan.
Background and objectives: Urinary tract infections are one of the world's major health problems. In addition, clinical disorders may result from the presence of bacteria or fungi in urine. The aim of this study was to isolate Escherichia coli (E. coli) strains from midstream urine samples, and to determine molecular characterization of encoded Extended Spectrum Beta-Lactamase (ESBL) genes.
Materials and methods: Collected urine samples were streaked on MacConkey, blood and EMB agar plates, then identifying E. coli isolates by using antibiotic susceptibility tests. ESBL production was measured using double disc diffusion. Furthermore, uniplex PCR was performed to identify two ESBL genes (blaCTX and blaTEM).
Results: Among 412 isolates, 198 (48.1%) were E. coli strains, followed by Staphylococcus saprophyticus, Klebsiella sp., Serratia sp., Enterococci sp. and Proteus sp. with frequency of 132 (32.0%), 51 (12.4%), 15 (3.6%), 10 (2.4%), and 6 (1.5%) respectively. Female participants who were between the ages of 40 and 49 years old, married, and pregnant were more likely to develop urinary tract infections (UTIs). E. coli species were present in 189 (95.5) of the recurrent UTIs. Regarding antimicrobial susceptibility testing of E. coli isolates, the highest percentage of resistance and susceptible rates were found for nalidixic acid (75.8%) and gentamicin (64.1%) respectively. Among the E. coli isolates, 25 (12.6%) were ESBL-producers. The blaCTX-M gene was genetically confirmed in 20 (10.1%) of the isolates.
Conclusion: E. coli is the most common cause of UTI and ESBL production leads to increased resistance to common antibiotics and complicates treatment strategies.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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