Mahtab Hosseinzadeh, Monir Doudi, Ali Mohammad Ahadi
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引用次数: 0
Abstract
Background and objectives: Bacteriocins are interested as antibacterial and anticancer agents due to their high specificity and low side effects. This study aimed to isolate bacteria which produce bacteriocins of the lacticin family from whey and to investigate their antibacterial and anticancer effects.
Materials and methods: Lactic acid bacteria were isolated from different whey samples. The presence of the lacticin gene in the isolates was checked using PCR and then the inhibitory effects of their bacteriocin was investigated on Staphylococcus aureus utilizing well plate method. The protein content was separated by dialysis. The presence of lacticin was checked with the help of SDS-PAGE. The lacticin producing bacterium was identified through the sequencing of the 16S rRNA gene. Finally, the cytotoxicity of the obtained protein was studied on the MCF-7 breast cancer cells using MTT and scratching tests.
Results: The isolated lacticin-producing Lactococcus lactis was able to grow in acidic conditions (pH = 2.5 for 3 h) and in bile salts (0.3% for 24 h). The bacterium produced 4.2 μg/μl bacteriocin with a molecular weight of 3.1 KD. The lacticin showed antibacterial effect against S. aureus. The cancerous cells treated with lacticin had slower growth than the control in Scratch test. Based on the MTT results, more than 80% of cancerous cells were inhibited at a concentration of 7 μg/ml lacticin with IC = 5.2 μg/ml.
Conclusion: The bacteriocin produced in this study is a promising antibacterial and anticancer agent.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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