{"title":"伊拉克Thi-Qar产碳青霉烯酶大肠杆菌临床分离株的表型和基因型特征","authors":"Moslim Mohsin Khalaf, Firas Srhan Abd Al-Mayahi","doi":"10.18502/ijm.v17i2.18387","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>The emergence of carbapenem resistance in <i>Escherichia coli (E. coli)</i> poses an urgent threat. The study aims to assess carbapenem resistance and the presence of carbapenemase genes in <i>E. coli</i> clinical isolates from Thi-Qar Hospital, Iraq.</p><p><strong>Materials and methods: </strong>A total of 2203 specimens were collected from patients at two hospitals between January and October 2024. <i>E. coli</i> was identified via biochemical tests and confirmed with the Vitek2® system. Antibiotic sensitivity was evaluated using disc diffusion, and carbapenemase production was investigated through combined disc tests (CDT) and modified Hodge tests (MHT). PCR was used to detect carbapenemase genes.</p><p><strong>Results: </strong>Out of 2203 specimens, 1212 (55.02%) exhibited bacterial growth, with <i>E. coli</i> accounting for 15.35% (186/1212) of isolates. Among these, 40 (21.51%) were resistant to at least one carbapenem. CDT identified 10, and MHT identified 1 as a carbapenemase producer. The most detected gene was <i>bla</i> <sub>NDM</sub> (60.00%), followed by <i>bla</i> <sub>OXA</sub> (40.00%) and <i>bla</i> <sub>OXA-48</sub> (15.00%). <i>bla</i> <sub>OXA-51</sub> and <i>bla</i> <sub>VIM</sub> were found in 5.00% of isolates each. No <i>bla</i> <sub>KPC</sub>, <i>bla</i> <sub>NMC</sub>, <i>bla</i> <sub>IMI</sub>, <i>bla</i> <sub>GES</sub>, <i>bla</i> <sub>SPM</sub>, <i>bla</i> <sub>GIM</sub>, or <i>bla</i> <sub>SIM</sub> was detected.</p><p><strong>Conclusion: </strong>The high prevalence of carbapenem resistance and the corresponding encoding genes in <i>E. coli</i> in Thi-Qar province pose a concerning challenge for managing serious infections caused by this pathogen.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"268-277"},"PeriodicalIF":1.3000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phenotypic and genotypic characterization of carbapenemase-producing <i>Escherichia coli</i> clinical isolates in Thi-Qar, Iraq.\",\"authors\":\"Moslim Mohsin Khalaf, Firas Srhan Abd Al-Mayahi\",\"doi\":\"10.18502/ijm.v17i2.18387\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>The emergence of carbapenem resistance in <i>Escherichia coli (E. coli)</i> poses an urgent threat. The study aims to assess carbapenem resistance and the presence of carbapenemase genes in <i>E. coli</i> clinical isolates from Thi-Qar Hospital, Iraq.</p><p><strong>Materials and methods: </strong>A total of 2203 specimens were collected from patients at two hospitals between January and October 2024. <i>E. coli</i> was identified via biochemical tests and confirmed with the Vitek2® system. Antibiotic sensitivity was evaluated using disc diffusion, and carbapenemase production was investigated through combined disc tests (CDT) and modified Hodge tests (MHT). PCR was used to detect carbapenemase genes.</p><p><strong>Results: </strong>Out of 2203 specimens, 1212 (55.02%) exhibited bacterial growth, with <i>E. coli</i> accounting for 15.35% (186/1212) of isolates. Among these, 40 (21.51%) were resistant to at least one carbapenem. CDT identified 10, and MHT identified 1 as a carbapenemase producer. The most detected gene was <i>bla</i> <sub>NDM</sub> (60.00%), followed by <i>bla</i> <sub>OXA</sub> (40.00%) and <i>bla</i> <sub>OXA-48</sub> (15.00%). <i>bla</i> <sub>OXA-51</sub> and <i>bla</i> <sub>VIM</sub> were found in 5.00% of isolates each. No <i>bla</i> <sub>KPC</sub>, <i>bla</i> <sub>NMC</sub>, <i>bla</i> <sub>IMI</sub>, <i>bla</i> <sub>GES</sub>, <i>bla</i> <sub>SPM</sub>, <i>bla</i> <sub>GIM</sub>, or <i>bla</i> <sub>SIM</sub> was detected.</p><p><strong>Conclusion: </strong>The high prevalence of carbapenem resistance and the corresponding encoding genes in <i>E. coli</i> in Thi-Qar province pose a concerning challenge for managing serious infections caused by this pathogen.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"17 2\",\"pages\":\"268-277\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v17i2.18387\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v17i2.18387","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Phenotypic and genotypic characterization of carbapenemase-producing Escherichia coli clinical isolates in Thi-Qar, Iraq.
Background and objectives: The emergence of carbapenem resistance in Escherichia coli (E. coli) poses an urgent threat. The study aims to assess carbapenem resistance and the presence of carbapenemase genes in E. coli clinical isolates from Thi-Qar Hospital, Iraq.
Materials and methods: A total of 2203 specimens were collected from patients at two hospitals between January and October 2024. E. coli was identified via biochemical tests and confirmed with the Vitek2® system. Antibiotic sensitivity was evaluated using disc diffusion, and carbapenemase production was investigated through combined disc tests (CDT) and modified Hodge tests (MHT). PCR was used to detect carbapenemase genes.
Results: Out of 2203 specimens, 1212 (55.02%) exhibited bacterial growth, with E. coli accounting for 15.35% (186/1212) of isolates. Among these, 40 (21.51%) were resistant to at least one carbapenem. CDT identified 10, and MHT identified 1 as a carbapenemase producer. The most detected gene was blaNDM (60.00%), followed by blaOXA (40.00%) and blaOXA-48 (15.00%). blaOXA-51 and blaVIM were found in 5.00% of isolates each. No blaKPC, blaNMC, blaIMI, blaGES, blaSPM, blaGIM, or blaSIM was detected.
Conclusion: The high prevalence of carbapenem resistance and the corresponding encoding genes in E. coli in Thi-Qar province pose a concerning challenge for managing serious infections caused by this pathogen.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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