Anti-candida activity of Lacticaseibacillus rhamnosus R-2002 and its possible application in candidiasis prevention.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI:10.18502/ijm.v17i3.18827

Aleksanyan Tigran, Hakobyan Lusine, Harutyunyan Ruzanna, Semerjyan Inesa, Nahapetyan Nelli, Gharibyan Edita, Haertlé Thomas, Bazukyan Inga

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引用次数: 0

Abstract

Background and objectives: Probiotics are used for the treatment of yeast infections, they restore the balance in vaginal microbiome, adhere to epithelial cells, compete against pathogenic bacteria, acidify the environment, produce bacteriocins and modulate the immunity. The aim of the study was to investigate the anti-yeast activity (AYA) of the strain Lacticaseibacillus rhamnosus R-2002 against different Candida species.

Materials and methods: From 20 strains of lactic acid bacteria examined, only L. rhamnosus R-2002 strain demonstrated beneficial properties against yeast. The effects of temperature and pH on AYA and its relation to cell wall were revealed by bi-layer agar assay. The connection of AYA to the cell wall was determined with the sonicated cells.

Results: R-2002 inhibited the growth of C. albicans ATCC 10291, C. tropicalis G 31 and C. albicans G4 (both isolated from vaginal samples). R-2002 maintained its AYA between a wide range of pH and its anti-yeast component/s are extracellular. The tested strain demonstrated stability against the high concentrations of progesterone and metronidazole, making it a suitable candidate for the mitigation of vaginitis.

Conclusion: The present study summarizes all the positive features of the strain R-2002 and its potential as a therapeutic agent in the treatment of candidiasis.

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鼠李糖乳杆菌R-2002抗念珠菌活性及其在念珠菌病防治中的应用前景。

背景和目的：益生菌用于治疗酵母菌感染，它们恢复阴道微生物群的平衡，粘附上皮细胞，与致病菌竞争，酸化环境，产生细菌素，调节免疫力。研究了鼠李糖乳杆菌R-2002对不同念珠菌的抑菌活性。材料与方法：在20株乳酸菌中，只有L. rhamnosus R-2002菌株显示出对酵母菌有益的特性。通过双层琼脂实验揭示了温度和pH对AYA的影响及其与细胞壁的关系。用超声细胞测定了AYA与细胞壁的连接。结果：R-2002抑制白色念珠菌ATCC 10291、热带念珠菌g31和白色念珠菌G4（均从阴道样本中分离）的生长。R-2002在较宽的pH范围内维持其AYA，其抗酵母成分/s在细胞外。该菌株对高浓度黄体酮和甲硝唑表现出稳定性，使其成为缓解阴道炎的合适人选。结论：本研究总结了菌株R-2002的所有阳性特征及其作为治疗念珠菌病药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.