{"title":"幽门螺杆菌源外膜囊泡（OMVs）对4T1乳腺癌细胞Snail/β-Catenin级联及转移相关蛋白的影响","authors":"Seyedeh Shadi Vaziri, Elahe Tajbakhsh, Faham Khamesipour, Hassan Momtaz","doi":"10.18502/ijm.v17i3.18824","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>This study investigates the impact of <i>Helicobacter pylori (H. pylori)</i>-derived outer membrane vesicles (OMVs) on the regulation of Snail/β-Catenin cascade and the production of metastasis-related proteins, such as E-cadherin and Vimentin, in the 4T1 cell line.</p><p><strong>Materials and methods: </strong>OMVs were purified from <i>H. pylori</i> (ATCC 700392) cultures and applied to 4T1 cells at concentrations of 1, 5, and 10 μg/mL, with untreated cells serving as controls. The MTT assay was employed to quantify cell viability. Expression profiles of +<i>vimentin, Snail, α-SMA</i>, and <i>β-catenin</i> genes were evaluated by qRT-PCR, while protein expression of E-cadherin and Vimentin was analyzed via immunohistochemistry. Data were analyzed using appropriate statistical methods with SPSS and GraphPad Prism software.</p><p><strong>Results: </strong>The MTT assay showed that 1 μg/mL OMVs were safe for normal cells. At this concentration, the expression of <i>vimentin, Snail, α-SMA</i>, and <i>β-catenin</i> genes significantly increased in the treatment group (P≤0.05). Additionally, Vimentin protein decreased, and E-cadherin protein increased (P≤0.05).</p><p><strong>Conclusion: </strong><i>H. pylori</i>-derived OMVs activate the Snail/β-Catenin cascade, influencing inflammatory responses and metastasis-related proteins, ultimately reducing migration in advanced cancer stages by modulating Vimentin and E-cadherin expression.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 3","pages":"411-419"},"PeriodicalIF":1.7000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218887/pdf/","citationCount":"0","resultStr":"{\"title\":\"Influence of <i>Helicobacter pylori</i>-derived outer membrane vesicles (OMVs) on Snail/β-Catenin cascade and metastasis-related proteins in 4T1 breast cancer cells.\",\"authors\":\"Seyedeh Shadi Vaziri, Elahe Tajbakhsh, Faham Khamesipour, Hassan Momtaz\",\"doi\":\"10.18502/ijm.v17i3.18824\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>This study investigates the impact of <i>Helicobacter pylori (H. pylori)</i>-derived outer membrane vesicles (OMVs) on the regulation of Snail/β-Catenin cascade and the production of metastasis-related proteins, such as E-cadherin and Vimentin, in the 4T1 cell line.</p><p><strong>Materials and methods: </strong>OMVs were purified from <i>H. pylori</i> (ATCC 700392) cultures and applied to 4T1 cells at concentrations of 1, 5, and 10 μg/mL, with untreated cells serving as controls. The MTT assay was employed to quantify cell viability. Expression profiles of +<i>vimentin, Snail, α-SMA</i>, and <i>β-catenin</i> genes were evaluated by qRT-PCR, while protein expression of E-cadherin and Vimentin was analyzed via immunohistochemistry. Data were analyzed using appropriate statistical methods with SPSS and GraphPad Prism software.</p><p><strong>Results: </strong>The MTT assay showed that 1 μg/mL OMVs were safe for normal cells. At this concentration, the expression of <i>vimentin, Snail, α-SMA</i>, and <i>β-catenin</i> genes significantly increased in the treatment group (P≤0.05). Additionally, Vimentin protein decreased, and E-cadherin protein increased (P≤0.05).</p><p><strong>Conclusion: </strong><i>H. pylori</i>-derived OMVs activate the Snail/β-Catenin cascade, influencing inflammatory responses and metastasis-related proteins, ultimately reducing migration in advanced cancer stages by modulating Vimentin and E-cadherin expression.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"17 3\",\"pages\":\"411-419\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218887/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v17i3.18824\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v17i3.18824","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Influence of Helicobacter pylori-derived outer membrane vesicles (OMVs) on Snail/β-Catenin cascade and metastasis-related proteins in 4T1 breast cancer cells.
Background and objectives: This study investigates the impact of Helicobacter pylori (H. pylori)-derived outer membrane vesicles (OMVs) on the regulation of Snail/β-Catenin cascade and the production of metastasis-related proteins, such as E-cadherin and Vimentin, in the 4T1 cell line.
Materials and methods: OMVs were purified from H. pylori (ATCC 700392) cultures and applied to 4T1 cells at concentrations of 1, 5, and 10 μg/mL, with untreated cells serving as controls. The MTT assay was employed to quantify cell viability. Expression profiles of +vimentin, Snail, α-SMA, and β-catenin genes were evaluated by qRT-PCR, while protein expression of E-cadherin and Vimentin was analyzed via immunohistochemistry. Data were analyzed using appropriate statistical methods with SPSS and GraphPad Prism software.
Results: The MTT assay showed that 1 μg/mL OMVs were safe for normal cells. At this concentration, the expression of vimentin, Snail, α-SMA, and β-catenin genes significantly increased in the treatment group (P≤0.05). Additionally, Vimentin protein decreased, and E-cadherin protein increased (P≤0.05).
Conclusion: H. pylori-derived OMVs activate the Snail/β-Catenin cascade, influencing inflammatory responses and metastasis-related proteins, ultimately reducing migration in advanced cancer stages by modulating Vimentin and E-cadherin expression.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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