Influence of Helicobacter pylori-derived outer membrane vesicles (OMVs) on Snail/β-Catenin cascade and metastasis-related proteins in 4T1 breast cancer cells.

IF 1.7 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2025-06-01 DOI:10.18502/ijm.v17i3.18824

Seyedeh Shadi Vaziri, Elahe Tajbakhsh, Faham Khamesipour, Hassan Momtaz

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引用次数: 0

Abstract

Background and objectives: This study investigates the impact of Helicobacter pylori (H. pylori)-derived outer membrane vesicles (OMVs) on the regulation of Snail/β-Catenin cascade and the production of metastasis-related proteins, such as E-cadherin and Vimentin, in the 4T1 cell line.

Materials and methods: OMVs were purified from H. pylori (ATCC 700392) cultures and applied to 4T1 cells at concentrations of 1, 5, and 10 μg/mL, with untreated cells serving as controls. The MTT assay was employed to quantify cell viability. Expression profiles of +vimentin, Snail, α-SMA, and β-catenin genes were evaluated by qRT-PCR, while protein expression of E-cadherin and Vimentin was analyzed via immunohistochemistry. Data were analyzed using appropriate statistical methods with SPSS and GraphPad Prism software.

Results: The MTT assay showed that 1 μg/mL OMVs were safe for normal cells. At this concentration, the expression of vimentin, Snail, α-SMA, and β-catenin genes significantly increased in the treatment group (P≤0.05). Additionally, Vimentin protein decreased, and E-cadherin protein increased (P≤0.05).

Conclusion: H. pylori-derived OMVs activate the Snail/β-Catenin cascade, influencing inflammatory responses and metastasis-related proteins, ultimately reducing migration in advanced cancer stages by modulating Vimentin and E-cadherin expression.

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幽门螺杆菌源外膜囊泡（OMVs）对4T1乳腺癌细胞Snail/β-Catenin级联及转移相关蛋白的影响

背景与目的：本研究探讨幽门螺杆菌（Helicobacter pylori， H. pylori）源性外膜囊泡（outer membrane vesicles, OMVs）对4T1细胞系Snail/β-Catenin级联反应以及E-cadherin和Vimentin等转移相关蛋白产生的影响。材料和方法：从幽门螺杆菌（ATCC 700392）培养物中纯化omv，分别以1、5和10 μg/mL的浓度作用于4T1细胞，未处理的细胞作为对照。MTT法测定细胞活力。qRT-PCR检测+vimentin、Snail、α-SMA、β-catenin基因表达谱，免疫组化检测E-cadherin、vimentin蛋白表达谱。采用SPSS和GraphPad Prism软件对数据进行统计学分析。结果：MTT试验显示，1 μg/mL的omv对正常细胞是安全的。在该浓度下，治疗组vimentin、Snail、α-SMA、β-catenin基因表达显著升高（P≤0.05）。Vimentin蛋白降低，E-cadherin蛋白升高（P≤0.05）。结论：幽门螺杆菌衍生的omv激活Snail/β-Catenin级联，影响炎症反应和转移相关蛋白，最终通过调节Vimentin和E-cadherin的表达减少晚期癌症的迁移。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.