Iranian Journal of Microbiology最新文献_第2页

New nano-chemotherapeutic chitosans-garlic oil-antibiotics against diabetic foot virulent Proteus spp. 新型纳米化学治疗壳聚糖-大蒜油-抗生素对糖尿病足带毒变形杆菌的作用

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16802

Khouloud M Barakat, Yousry M Gohar, Hasnaa E-B Ghonam, Ghada A Bashir

{"title":"New nano-chemotherapeutic chitosans-garlic oil-antibiotics against diabetic foot virulent Proteus spp.","authors":"Khouloud M Barakat, Yousry M Gohar, Hasnaa E-B Ghonam, Ghada A Bashir","doi":"10.18502/ijm.v16i5.16802","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16802","url":null,"abstract":"Background and objectives: Diabetes foot ulcer is recognized to have a major side effect that raises the risk of amputation. Diabetic ulcer bacterial infections caused by virulent and resistant bacteria like Proteus mirabilis lead to serious wounds that are incurable with conventional medications.Materials and methods: In this study, we evaluated the antibacterial activity of a natural product nanochitosan - garlic oil against ten diabetic foot isolates of Proteus mirabilis. Various chitosans (Crab (CScr) - shrimp (CSsh) - squilla (CSsq)) in nano form were prepared and coated with garlic oil (GO). GC-MS analysis was carried out to determine the main components of the essential garlic oil. The physicochemical properties of GO-NCSsq were analyzed using dynamic light scattering (DLS), zeta potentials (ZP) and subsequently scan electron microscope (SEM). Additionally, the minimum inhibitory concentration (MIC) and fraction inhibitory concentration index (FICI) were determined.Results: GC-MS analysis revealed the presence of major palmitic fatty acid. (GO) loaded on nanochitosan squilla (NCSsq) showed high activity. Although SEM showed lower nano-size, average size of the GO-NCSsq was 330.8 nm by DLS and its zeta potential formulation was +39.6 mV. The final formulation represented by GO-NCSsq + Pipercillin (Pi) inhibited the virulence factor of P. mirabilis and reduced the MIC value (p-value > 0.001). Moreover, the killing time at 9 h was found to be significantly higher for GO-NCSsq + pipercillin (Pi) against P. mirabilis.Conclusion: In order to manage diabetic foot infections, GO-NCSsq is a legitimate antibacterial agent that can be coupled with other antibiotics.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"666-675"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of chitosan nanogels loaded with vancomycin and gamma interferon on TNF-α gene expression in macrophage cell line activated with methicillin-resistant Staphylococcus aureus (MRSA). 载入万古霉素和γ干扰素的壳聚糖纳米凝胶对耐甲氧西林金黄色葡萄球菌（MRSA）激活的巨噬细胞系中 TNF-α 基因表达的影响。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16794

Sahar Arkiya, Ardeshir Hesampour, Ali Esrafili, Javad Arasteh

{"title":"Effect of chitosan nanogels loaded with vancomycin and gamma interferon on TNF-α gene expression in macrophage cell line activated with methicillin-resistant Staphylococcus aureus (MRSA).","authors":"Sahar Arkiya, Ardeshir Hesampour, Ali Esrafili, Javad Arasteh","doi":"10.18502/ijm.v16i5.16794","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16794","url":null,"abstract":"Background and objectives: Staphylococcus aureus is an opportunistic pathogen that frequently leads to asymptomatic infections. Methicillin-resistant strains (MRSA) pose a significant threat as they are resistant to most commonly used antibiotics, complicating treatment efforts. This study aimed to develop chitosan nanogels loaded with vancomycin and IFN-γ and to assess the expression of the TNF-α gene in a cell line infected with MRSA.Materials and methods: Following the synthesis and confirmation of the chitosan nanogels, vancomycin and IFN-γ were incorporated into these nanogels. The synthesis was validated using DLS, FTIR, TEM, and SEM. Subsequently, the anti-bacterial efficacy of the nanogels was assessed. Finally, four groups of cell lines were designed: control, MRSA, chitosan nanogels and IFN-γ-vancomycin chitosan nanogels. After infection of the groups (except control) with MRSA, 5 μg/mL of nanogels, and nanogels (drug and IFN-γ) were added to groups 3 and 4, respectively. Then the expression of TNF-α gene in each group was analyzed by RT-PCR at 6 and 24 hours.Results: At pH 6.5 and 7.4, the MIC of 1 μg/mL was obtained for free vancomycin, whereas that of IFN-γ-vancomycin nanogels at both pHs was respectively 8 and 64 μg/mL. The IC50 of chitosan nanogels and nanogels loaded with vancomycin-IFN-γ on RAW264.7 cells were 2.37 and 4.15 μg/mL in 24 hours, respectively. In group 4 in comparison to the MRSA group, TNF-α expression decreased significantly following 24 hours.Conclusion: Loading of vancomycin and IFN-γ in the chitosan nanogel can reduce TNF-α gene expression on MRSA infected cell lines.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"614-623"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MIC trends of vancomycin and teicoplanin among methicillin resistant CoNS isolates from new born blood cultures in a tertiary care centre in Southern India. 印度南部一家三级医疗中心新生儿血液培养物中耐甲氧西林的 CoNS 分离物中万古霉素和替考拉宁的 MIC 变化趋势。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16792

Neethu John, Chinnu Sajeev, Greeshma Hareendranath, Tanya Tonny Mampilly, Aswathy Varghese, Lancy Justus

{"title":"MIC trends of vancomycin and teicoplanin among methicillin resistant CoNS isolates from new born blood cultures in a tertiary care centre in Southern India.","authors":"Neethu John, Chinnu Sajeev, Greeshma Hareendranath, Tanya Tonny Mampilly, Aswathy Varghese, Lancy Justus","doi":"10.18502/ijm.v16i5.16792","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16792","url":null,"abstract":"Background and objectives: Coagulase-negative-Staphylococci (CoNS) are important etiological agent of bacteraemia in newborn babies. Methicillin resistant CoNS infections have only limited treatment options. The antimicrobial susceptibility pattern of Methicillin Resistant CoNS isolates from newborn blood cultures was studied with special reference to MICs of Vancomycin and Teicoplanin.Materials and methods: The study population included Methicillin Resistant Coagulase Negative Staphylococcal isolates (MRCoNS) from newborn blood cultures, during a one-year period. Minimum inhibitory concentration (MIC) of Vancomycin and Teicoplanin in Methicillin resistant CoNS isolates was determined by macrobroth dilution method as per CLSI guidelines and by automated methods.Results: Coagulase Negative Staphylococci were the etiological agent in 73.7% (n=56) cases of neonatal bacteremia. Methicillin resistance in newborn CoNS was found to be 58.9%. All the MRCoNS isolates had vancomycin and teicoplanin MICs in the susceptible range. There were MRCoNS isolates with MICs in the upper limit of susceptible range for both vancomycin and teicoplanin, which can result in poor clinical response.Conclusion: Continuous large scale multi-centre surveillance studies with special attention to study the MIC pattern of the high-end anti-MRSA agents like vancomycin, teicoplanin, linezolid are to be carried out. This will help the clinicians to judiciously prescribe the antibiotics, which is very essential for antimicrobial stewardship.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"598-604"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142619644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus dysgalactiae subsp.-equisimilis as an emerging secondary pathogen in leprosy foot ulcers. 麻风脚溃疡中新出现的继发病原体--赤痢链球菌亚种（Streptococcus dysgalactiae subsp.-equisimilis）。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16795

Kumar Ebineshan, Michael Sukumar Pallapati, Aparna Srikantam

{"title":"Streptococcus dysgalactiae subsp.-equisimilis as an emerging secondary pathogen in leprosy foot ulcers.","authors":"Kumar Ebineshan, Michael Sukumar Pallapati, Aparna Srikantam","doi":"10.18502/ijm.v16i5.16795","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16795","url":null,"abstract":"Background and objectives: Leprosy foot ulcers (LFU) tend to become chronic due to secondary bacterial infections, leading to subsequent disfigurement and disability. Treatment modality for infected plantar ulcers thus so far is majorly based on conventional approach of empirical antibacterial therapy. However, this approach tends to overlook unconventional pathogens which are likely to be present in the LFU.Materials and methods: Twenty-six leprosy patients (17 males and 9 females) who had completed multidrug therapy (MDT) and those are suffering from foot ulcer were included. Using sterile cotton swabs, two wound swabs were collected, of these; one for bacterial culture and another for NGS (Next Generation Sequencing).Results: Out of 26 samples tested on conventional bacterial culture, Streptococcus spp. (50%) was predominant organism. On NGS, 09/26 (34.61%) showed Streptococcus-dysgalactiae-subsp.-equisimilis-GGS 12 as the most abundant single organism, along with some unknown and unclassified organisms; 03/26 (11.5%) were Arcanobacterium-haemolyticum-DSM-20595 alone and 02/26 (7.69%) were Streptococcus-pyogenes alone.Acombination of Arcanobacterium-haemolyticum-DSM-20595 and Streptococcus-dysgalactiae-subsp.-equisimilis-GGS 124 was found in nine (34.61%) specimens.Conclusion: Polymicrobial infection with conventional and unconventional pathogenic bacteria is another notable finding suggesting appropriate interventions. The study findings also reiterate the need for understanding the polymicrobial infections and their role in the clinical progression of the LFU.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"624-630"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adenovirus-5 and adenovirus-37 seropositivity in obese patients. 肥胖患者的腺病毒-5 和腺病毒-37 血清阳性反应。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16804

Nur Gamze Bostan, Ozge Altinok, Buyukasik Süleyman, Halil Alis, Yasar Ali Oner

{"title":"Adenovirus-5 and adenovirus-37 seropositivity in obese patients.","authors":"Nur Gamze Bostan, Ozge Altinok, Buyukasik Süleyman, Halil Alis, Yasar Ali Oner","doi":"10.18502/ijm.v16i5.16804","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16804","url":null,"abstract":"Background and objectives: Obesity is a major health issue linked to conditions like diabetes, hypertension, and hyperlipidemia. Infectobesity suggests that certain microorganisms may contribute to obesity. Human adenovirus serotypes, particularly Human adenovirus type-36 (HAdV-36), Human adenovirus type-5 (HAdV-5), and Human adenovirus type-37 (HAdV-37), are thought to influence body fat regulation. This study investigates the relationship between Immunoglobulin G (IgG) positivity for HAdV-5 and HAdV-37 and obesity, aiming to provide data on the infectious etiology of obesity.Materials and methods: Blood samples separated into serums from obese (BMI ≥30) and non-obese (BMI 18.5-25) individuals were tested for HAdV-5 and HAdV-37 seropositivity using ELISA kits and seropositivity rates between the groups were compared.Results: HAdV-37 antibody positivity was significantly higher in obese patients (39/48) compared to the control group (24/42) (p=0.011). For HAdV-5, antibody positivity was similar in both groups (26 individuals each) with no significant difference (p=0.461). No significant gender-related differences were found for either serotype.Conclusion: The study suggests HAdV-37 may be associated with obesity, while no such relationship was found for HAdV-5. There was no gender association for either serotype. These results align with existing literature on HAdV-37, but further research is needed to confirm the link between adenoviruses and obesity and explore potential treatment options.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"684-688"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth response of Vibrio cholerae O1 and V. cholerae non O1/non O139 strains to algae extracts from stream water in far north Cameroon. 霍乱弧菌O1和霍乱弧菌非O1/非O139菌株对喀麦隆最北部溪水中藻类提取物的生长反应。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI: 10.18502/ijm.v16i5.16796

Moussa Djaouda, Roméo Wakayansam Bouba, Pierre Nestor Nguimbous, Pagoui Ehbiakbo, Eric Moïse Bakwo Fils, Céline Nguefeu Nkenfou

{"title":"Growth response of Vibrio cholerae O1 and V. cholerae non O1/non O139 strains to algae extracts from stream water in far north Cameroon.","authors":"Moussa Djaouda, Roméo Wakayansam Bouba, Pierre Nestor Nguimbous, Pagoui Ehbiakbo, Eric Moïse Bakwo Fils, Céline Nguefeu Nkenfou","doi":"10.18502/ijm.v16i5.16796","DOIUrl":"https://doi.org/10.18502/ijm.v16i5.16796","url":null,"abstract":"Background and objectives: Vibrio cholerae O1 or non O1/non O139 is found in water ecosystems where it colonizes phytoplankton and has different lifestyle. This study aimed to investigate the impact of some algae extracts on the survival/growth of both V. cholerae strains.Materials and methods: Algae extracts consisting of three fractions, F1 containing chlorophyll-a, F2 containing chlorophyll-b, and F3 containing carotenoids, and raw extract (RAE) were obtained from the algal bloom collected in the Kaliao stream (Maroua, Cameroon). The survival and growth of V. cholerae O1 and V. cholerae non O1/non O139, in microcosms consisting of sterile saline with these extracts and peptone (PEP) respectively added at concentrations of 0.01, 0.05 and 0.1 mg/L, and 50/50 mixtures F1+F2, F2+F3, and F2+PEP at a concentration 0.05 mg/L, were compared during a 24h experiment.Results: The microcosms F2 and RAE did not support the growth of O1 strain; V. cholerae non O1/non O139 count in all algae extract microcosms ranging from 3.97 log (CFU/mL) to 5.2 log (CFU/mL). In all PEP microcosms, the counts of both strains reached an uncountable value. Microcosms F1+F2 and F2+F3 supported the growth of V. cholerae O1 and V. cholerae non-O1/nonO139 strains.Conclusion: The algae compounds showed strain-specific effect on the growth of V. cholerae.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 5","pages":"631-638"},"PeriodicalIF":1.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of Nigella sativa oil loaded to liposomal nanocarriers on Candida parapsilosis isolates. 负载于脂质体纳米载体的黑升麻油对副丝状念珠菌分离物的抑制作用。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-08-01 DOI: 10.18502/ijm.v16i4.16316

Ardalan Ghiaee Shamloo, Hossein Zarrinfar, Mahmoud Reza Jaafari, Mohammad Hossein Yadegari

{"title":"Inhibitory effect of Nigella sativa oil loaded to liposomal nanocarriers on Candida parapsilosis isolates.","authors":"Ardalan Ghiaee Shamloo, Hossein Zarrinfar, Mahmoud Reza Jaafari, Mohammad Hossein Yadegari","doi":"10.18502/ijm.v16i4.16316","DOIUrl":"https://doi.org/10.18502/ijm.v16i4.16316","url":null,"abstract":"Background and objectives: Candida parapsilosis is the second most common species causing infectious diseases and can lead to biofilm resistance. This study aims to adjust and synthesize a liposomal compound of Nigella sativa and evaluate its antifungal properties against C. parapsilosis isolates.Materials and methods: The liposomal formulation of N. sativa was optimized through the utilization of transmission electron microscopy (TEM), particle size analysis, zeta potential measurement, and UV-visible spectrophotometry. Furthermore, an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was conducted on peripheral blood mononuclear cells (PBMCs). The antifungal efficacy was evaluated in accordance with the M27-A3 guideline.Results: The minimum inhibitory concentrations (MICs) of N. sativa oil and the liposomal formulation on C. parapsilosis isolates ranged from 128 to 8 µg/mL and from 250 to 31.25 µg/mL, respectively. The MIC50 and MIC90 values of N. sativa oil and the liposomal formulation were 125, 187, and 32, 96 µg/mL, respectively. The viability percentage of cells treated with the liposomal formulation and free N. sativa oil was 91% and 85%, respectively.Conclusion: The cytotoxicity of free N. sativa was significantly reduced when using nanoliposomes. The liposomal form of N. sativa showed greater antifungal properties compared to the free N. sativa extract against C. parapsilosis isolates.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 4","pages":"560-568"},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and diversity of enteric Helicobacter spp. in healthy and diarrheic cats. 健康猫和腹泻猫肠道螺旋杆菌的流行率和多样性。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-08-01 DOI: 10.18502/ijm.v16i4.16317

Reza Ahmadi, Farnoosh Arfaee, Shahram Jamshidi, Pejman Mortazavi, Mahmoud Jamshidian

{"title":"Prevalence and diversity of enteric Helicobacter spp. in healthy and diarrheic cats.","authors":"Reza Ahmadi, Farnoosh Arfaee, Shahram Jamshidi, Pejman Mortazavi, Mahmoud Jamshidian","doi":"10.18502/ijm.v16i4.16317","DOIUrl":"https://doi.org/10.18502/ijm.v16i4.16317","url":null,"abstract":"Background and objectives: Helicobacters are gastric and enterohepatic and live in the gut. The role of enterohepatic Helicobacters as intestinal pathogens is uncertain, while stomach Helicobacters are well-known. The prevalence of Helicobacter species in cat feces helps us understand their impact on cat health and human disease transmission. This study used PCR to identify Helicobacter spp. in feces samples from healthy and diarrhoeic cats, independent of the reason. The study also compared intestinal and stomach Helicobacter species.Materials and methods: PCR analysis was performed on fecal samples from 40 cats, with 20 cats having diarrhea and 20 cats showing no symptoms. The PCR analysis aimed to detect Helicobacter's presence using a method that identifies the bacteria through the 16S rRNA gene.Results: The diarrhoeic group had a greater prevalence of infection (17:9 ratio), with an overall 65% infection rate detected. Cats that were older than 2 years showed a higher incidence of disease. H. canis had the highest occurrence rate (69.2%), followed by H. bilis, H. bizzozeronii, and H. salomonis. Significantly, H. pylori, H. felis, and H. heilmannii were not reported.Conclusion: H. canis was the predominant species found in both healthy and diarrheic cats, indicating the need for more investigation. The detection of the gastric species H. salomonis and H. bizzozeronii further complicates the classification. This highlights the complex nature of Helicobacter infections in cats, emphasizing the need for further investigation to guide the development of preventative measures and treatment techniques for both veterinary and public health purposes.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 4","pages":"569-575"},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic susceptibility and biofilm forming ability of Staphylococcus aureus isolated from Jordanian patients with diabetic foot ulcer. 从约旦糖尿病足溃疡患者中分离出的金黄色葡萄球菌的抗生素敏感性和生物膜形成能力。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-08-01 DOI: 10.18502/ijm.v16i4.16303

Dima Owais, Rania M Al-Groom, Tareq Nayef AlRamadneh, Laila Alsawalha, Mohd Sajjad Ahmad Khan, Omar H Yousef, Shereen Z Burjaq

{"title":"Antibiotic susceptibility and biofilm forming ability of Staphylococcus aureus isolated from Jordanian patients with diabetic foot ulcer.","authors":"Dima Owais, Rania M Al-Groom, Tareq Nayef AlRamadneh, Laila Alsawalha, Mohd Sajjad Ahmad Khan, Omar H Yousef, Shereen Z Burjaq","doi":"10.18502/ijm.v16i4.16303","DOIUrl":"https://doi.org/10.18502/ijm.v16i4.16303","url":null,"abstract":"Background and objectives: Microbial biofilm is characterized by the irreversible attachment of planktonic cells to a surface and is usually associated with high antimicrobial resistance with worsening the wound healing. The objective of the study was to determine the prevalence of Staphylococcus aureus in diabetic foot ulcers (DFUs) of diabetic patients and to investigate antibiotic susceptibility patterns of these isolates. In addition to screen biofilm forming ability of isolated S. aureus.Materials and methods: A total of 112 non-healing wound swabs of diabetic foot patients were collected and cultured on different culture media to identify and characterize 98 isolates. The S. aureus isolates were examined for their antibiotic susceptibility to different antimicrobial agents. Furthermore, S. aureus isolates were evaluated for their biofilm production capability using the Tissue Culture Plate Method (TPC). The level of icaA gene expression was determined by RT-PCR.Results: The results of this study showed that these non-healing wounds yield positive cultures, with an average of 1.67 organisms per sample. The isolates showed highest resistance against oxacillin (95.2%) and lowest resistance against linezolid (3.7%). All isolates were biofilm producers and a significant association with the icaA gene expression level was recorded.Conclusion: This study showed that S. aureus isolates have a great ability to produce biofilms that are associated with the chronicity of wounds in diabetic patients. Routine screening for biofilm formers in chronic wounds and their antibiotic susceptibility testing will help in early treatment and prevent any other complications.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 4","pages":"450-458"},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus associated with pyogenic infections. 与化脓性感染有关的耐甲氧西林金黄色葡萄球菌的表型和基因型特征。

IF 1.3

Iranian Journal of Microbiology Pub Date : 2024-08-01 DOI: 10.18502/ijm.v16i4.16302

Sharanya Krishnakumar, Abdul Azeez Mohamed Khalid, Jothipandian Sowndarya, Lakshmi Krishnasamy, Paramasivam Nithyanand

{"title":"Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus associated with pyogenic infections.","authors":"Sharanya Krishnakumar, Abdul Azeez Mohamed Khalid, Jothipandian Sowndarya, Lakshmi Krishnasamy, Paramasivam Nithyanand","doi":"10.18502/ijm.v16i4.16302","DOIUrl":"https://doi.org/10.18502/ijm.v16i4.16302","url":null,"abstract":"Background and objectives: Staphylococcal infections are one of the major infectious diseases affecting globally in spite of advances in development of antimicrobial agents. Knowledge and awareness about the local pattern and prevalence of MRSA infections plays a key role in treatment. The aim of this study was to identify MRSA strains by phenotypic and genotypic methods and to analyze the antibiotic susceptibility pattern of MRSA strains from patients attending a tertiary care hospital.Materials and methods: This study was conducted over a period of 1 year, where 296 isolates of Staphylococcus aureus were isolated from various clinical specimens. The isolated strains were examined for antibiotic susceptibility by the modified Kirby Bauer disc diffusion method. Methicillin resistance was detected by cefoxitin disk diffusion test.Results: A total of 104 isolates were found to be MRSA and 192 were found to be MSSA. Among the 104 MRSA isolates, 10 strains that were multidrug resistant were subjected to 16S rRNA gene sequencing analysis. All the 10 strains had a 99% match with S. aureus strains that were responsible for causing some serious biofilm mediated clinical manifestations like cystic fibrosis and device mediated infections. The biofilms were quantified using crystal violet staining and their ability to produce biofilms was analyzed using scanning electron microscopy and matched with the Genbank.Conclusion: Hence these phylogenetic analysis aid in treating the patients and combating resistance to antibiotics.","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 4","pages":"443-449"},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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