Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Multifunctional MeHA hydrogel for living materials delivery with enhanced cartilage regeneration.","authors":"Qunchao Chen, Lang Bai, Guoyang Wan, Yuefeng Hao, Xing Yang, Hongtao Zhang","doi":"10.3389/fbioe.2025.1545773","DOIUrl":"10.3389/fbioe.2025.1545773","url":null,"abstract":"<p><p>Particulated juvenile articular cartilage (PJAC) has emerged as a promising living material for articular defect treatment. However, the fragile nature of PJAC hinders its wide clinical application. Here, inspired by the chemical composition and hierarchical structure of natural cartilage, we developed a novel hydrogel carrier system for PJAC delivery. Our carrier system, MeHA@J@DM, utilized methacrylated hyaluronic acid (MeHA) to incorporate PJAC and coated it with a polymerized mixture of dopamine methacrylamide (DMA) and 2-methylacryloyloxyethyl phosphorylcholine (MPC), forming an adhesive lubricant, p(DMA-MPC). MeHA@J@DM exhibited excellent performance for PJAC protection with enhanced cell viability, bioactivity, and lubrication properties. We evaluated the effectiveness of MeHA@J@DM in cartilage cell migration, where juvenile cartilage showed greater efficiency and remodeling abilities. <i>In vivo</i> rabbit cartilage defect models demonstrated superior cartilage regeneration with the MeHA@J@DM hydrogel. Our findings suggest that MeHA@J@DM has translational potential for PJAC implantation to enhance cartilage regeneration and benefit patients with articular cartilage lesions.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1545773"},"PeriodicalIF":4.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive model establishment for forward-head posture disorder in primary-school-aged children based on multiple machine learning algorithms.","authors":"Hongjun Tao, Yang Wen, Rongfang Yu, Yining Xu, Fangliang Yu","doi":"10.3389/fbioe.2025.1607419","DOIUrl":"10.3389/fbioe.2025.1607419","url":null,"abstract":"<p><strong>Background: </strong>Forward head posture frequently occurs among primary school children, potentially due to prolonged sedentary behavior associated with academic demands and reduced physical activity. However, existing prevention and screening methods fail to accurately and promptly predict the onset of forward head posture.</p><p><strong>Objective: </strong>This study aims to identify highly sensitive predictive indicators for forward head posture in primary school children using the Least Absolute Shrinkage and Selection Operator (LASSO) regression algorithm. Multiple machine learning algorithms are applied to construct distinct risk prediction models, with the most effective model selected through comparative analysis. The Shapley Additive Explanations (SHAP) method is used to quantify the influence of each feature on model outcomes, ensuring enhanced model interpretability.</p><p><strong>Methods: </strong>Employing a cross-sectional study design, this research recruited 520 primary school-aged children, gathering data on demographics, anthropometrics, and physical activity levels. Univariate logistic regression was utilized to identify high-risk factors for forward head posture. The LASSO algorithm was subsequently applied to select key predictors. Six machine learning models-K-nearest neighbor (KNN), light gradient boosting machine (LGBM), extreme gradient boosting (XGBoost), random forest (RF), linear model (LM), and support vector machine (SVM)-were developed to predict risk. The performance of each model was evaluated, and the best-performing model was further interpreted using the Shapley Additive Explanations (SHAP) algorithm.</p><p><strong>Results: </strong>A total of 514 children were ultimately included in the study, of whom 300 exhibited forward head posture. LASSO analysis identified age, bodyweight, BMI, sex, and weekly total homework time as prominent risk indicators. Among the 6 predictive models, the random forest algorithm demonstrated the highest performance (AUC = 0.865), significantly outperforming the others. SHAP analysis revealed that BMI, bodyweight, and age were the most influential predictors, with BMI contributing the most.</p><p><strong>Conclusion: </strong>The random forest-based prediction model achieved superior predictive accuracy for forward head posture among Chinese primary school children, emphasizing the importance of monitoring BMI, bodyweight, and age for early intervention and prevention efforts.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1607419"},"PeriodicalIF":4.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical evaluation of the triangular support structure of the proximal femoral bionic nail compared to conventional long intramedullary nails for subtrochanteric fractures.","authors":"Yanjiang Yang, Dongwei Wu, Xiaodong Cheng, Wei He, Wei Chen, Yingze Zhang, Qi Zhang","doi":"10.3389/fbioe.2025.1579842","DOIUrl":"10.3389/fbioe.2025.1579842","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to compare the biomechanical results of long proximal femoral bionic nail (PFBN) and three conventional intramedullary nails in the treatment of subtrochanteric fractures (STFs).</p><p><strong>Methods: </strong>Using finite element analysis, we compared the therapeutic efficacy of four long intramedullary nails: the PFBN, reconstruction nail (RCN), InterTAN nail (ITN), and proximal femoral nail antirotation (PFNA) for the treatment of Seinsheimer type IIIA and type V STFs. The biomechanical stability of the implants was evaluated by calculating of von Mises stress (VMS), contact pressure and displacement for three loading scenarios.</p><p><strong>Results: </strong>The results showed that the PFBN group had the lowest VMS values under axial, bending and torsional loads. Under axial loading conditions, the VMS of PFBN was 480.04 MPa, followed by ITN (726.39 MPa), PFNA (730.48 MPa), and RCN (837.24 MPa) in the type V fracture groups. In the PFBN group, the contact pressure was 19.22 MPa and the tangential micromotion was 0.089 mm for the type IIIA group, 23.69 MPa and 0.08 mm for the type V group. Compared to the ITN, PFNA and RCN groups, the PFBN group exhibited the lowest contact pressure and tangential micromotion at the fracture sites.</p><p><strong>Conclusion: </strong>The superior biomechanical properties of the PFBN under axial, bending, and torsional loads not only reduced stress at the fracture site, but also improved structural stability.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1579842"},"PeriodicalIF":4.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon dots-based drug delivery for bone regeneration.","authors":"Christy Liu, Yingzi Li, Xiaohua Liu","doi":"10.3389/fbioe.2025.1613901","DOIUrl":"10.3389/fbioe.2025.1613901","url":null,"abstract":"<p><p>Carbon dots (CDs) are a class of nanobiomaterials with significant potential in bone regeneration. Their excellent biocompatibility, tunable fluorescence, high stability, low toxicity, and abundant functional groups make CDs promising candidates for efficient drug delivery and bone tissue regeneration. CDs contribute to targeted drug release, enhance osteogenic differentiation, and interact with cellular components to facilitate bone formation. Recent research highlights the roles of CDs in scaffold-based approaches, offering controlled drug delivery and real-time bioimaging capabilities. This review provides a comprehensive overview of CDs in bone regeneration, with a focus on their synthesis, functionalization, and biomedical applications. It begins by exploring CD synthesis methods, physicochemical properties, and mechanisms of action. Next, it discusses CD-based drug delivery systems and their applications in bone regeneration. Finally, the review highlights the challenges and future perspectives in optimizing CDs for enhanced therapeutic outcomes.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1613901"},"PeriodicalIF":4.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Image-based digital tools for diagnosis and surgical treatment: applications, challenges, and prospects.","authors":"Laura Cercenelli, Adrian Elmi-Terander, Thomas Maal, Virginia Mamone, Francesca Manni","doi":"10.3389/fbioe.2025.1597238","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1597238","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1597238"},"PeriodicalIF":4.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges in lentiviral vector production: retro-transduction of producer cell lines.","authors":"Maximilian Klimpel, Christin Obert, Monica Terrao, Parameswari Singh, Haiyan Wei, Chao-Guang Chen, Andreas Gille, Silke Wissing, Holger Laux","doi":"10.3389/fbioe.2025.1569298","DOIUrl":"10.3389/fbioe.2025.1569298","url":null,"abstract":"<p><p>The increasing demand for lentiviral vectors (LVs) has led to the development of several stable cell lines and production methods over the last 2 decades in order to increase titers and yields, reduce production costs and improve the safety of the vector product. However, the phenomenon of retro-transduction, which describes the transduction of LV producer cells by self-produced LVs, remains largely unaddressed in the context of LV production. Recent research has focused on various approaches to reduce the impact of retro-transduction on LV yield and process performance. This article reviews existing and new research data that highlights the impact of retro-transduction in LV manufacturing. In addition, a perspective on current advances to reduce retro-transduction is provided and a potential novel strategy called ENV-Y is presented, which could not only reduce the impact of retro-transduction but also facilitate the subsequent LV downstream process.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1569298"},"PeriodicalIF":4.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fully coupled fluid-structure interaction model for patient-specific analysis of bioprosthetic aortic valve haemodynamics.","authors":"Zhongjie Yin, Chlöe Armour, Selene Pirola, Harkamaljot Kandail, Xiaoxin Kan, Pankaj Garg, Rui Li, Toufan Bahrami, Saeed Mirsadraee, Xiao Yun Xu","doi":"10.3389/fbioe.2025.1584509","DOIUrl":"10.3389/fbioe.2025.1584509","url":null,"abstract":"<p><strong>Background: </strong>Bioprosthetic aortic valves (BPAV) have been increasingly used for surgical aortic valve replacement (SAVR), but long-term complications associated with structural valve deterioration remain a concern. The structural behaviour of the valve and its surrounding haemodynamics play a key role in the long-term outcome of SAVR, and these can be quantitively analysed by means of fluid-structure interaction (FSI) simulation. The aim of this study was to develop a fully coupled FSI model for patient-specific analysis of BPAV haemodynamics.</p><p><strong>Methods: </strong>Using the Edwards Magna Ease valve as an example, the workflow included reconstruction of the aortic root from CT images and the creation of valve geometric model based on available measurements made on the device. Two-way fully coupled FSI simulations were performed under patient-specific flow conditions derived from 4D flow magnetic resonance imaging (MRI), the latter also provided data for model validation.</p><p><strong>Results: </strong>The simulation results were in good agreement with haemodynamic features extracted from 4D flow MRI and relevant data in the literature. Furthermore, the FSI model provided additional information that cannot be measured <i>in vivo</i>, including wall shear stress and its derivatives on the valve leaflets and in the aortic root.</p><p><strong>Conclusion: </strong>The FSI workflow presented in this study offers a promising tool for patient-specific assessment of aortic valve haemodynamics, and the results may help elucidate the role of haemodynamics in structural valve deterioration.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1584509"},"PeriodicalIF":4.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12160531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed porous tantalum for acetabular reconstruction in complex primary arthroplasty and revision of hip.","authors":"Zonghan Wang, Ze Wang, Lingchuan Gu, Ying Zhang, Tiao Su, Jiangming Luo, Chengjun Huang, Xiaoyuan Gong, Yang Peng, Guangxing Chen","doi":"10.3389/fbioe.2025.1557882","DOIUrl":"10.3389/fbioe.2025.1557882","url":null,"abstract":"<p><strong>Introduction: </strong>In cases of hip joint damage, such as osteoarthritis (OA), rheumatoid arthritis (RA), avascular necrosis, or hip fractures, total hip arthroplasty (THA) is a critical surgical intervention. For individuals whose hip abnormalities stem from congenital issues, injuries, or previous operations, this procedure can encounter considerable obstacles, including complex bone defects, soft tissue deficiencies, and an increased risk of infections, which may result in poor alignment, joint instability, and higher need for revisions. This study explored the application of personalized, three-dimensional (3D)-printed porous tantalum buttresses designed specifically for acetabular reconstruction. Renowned for its compatibility with human biology, tantalum facilitates superior integration with natural bone.</p><p><strong>Methods: </strong>The development process started with the generation of meticulous computer-aided design (CAD) models, derived from preoperative imaging techniques such as computed tomography (CT) scans and (magnetic resonance imaging) MRIs, which allowed for the creation of components precisely matching each patient's unique anatomical structure. The 3D-printed porous tantalum buttresses were made by cutting-edge additive manufacturing methods. The porosity of the tantalum structure promoted the growth of new bone tissue into the implant, improving its stability and durability. During surgeries, the buttress was positioned to reconstruct the acetabulum, laying a solid foundation for the artificial hip joint.</p><p><strong>Results: </strong>The results of our study showed that all surgeries were successfully completed with no significant vascular or nerve damage. Postoperative evaluations showed that the buttress had excellent biomechanical function and firm fixation, with a large amount of bone ingrowth, improving the fitness and performance of the implant while reducing the possibility of subsequent problems such as loosening or dislocation.</p><p><strong>Discussion: </strong>This innovative technique has great potential in clinical practice for better outcomes and quality of life for patients with complex hip deformities.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1557882"},"PeriodicalIF":4.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of mental fatigue on biomechanical characteristics and risk associated with non-contact anterior cruciate ligament injuries during landing.","authors":"Bosong Zheng, Zeyang Zhang, Zeyi Zhang, Youping Sun, Yao Xiao, Mengjie Li","doi":"10.3389/fbioe.2025.1582873","DOIUrl":"10.3389/fbioe.2025.1582873","url":null,"abstract":"<p><strong>Objective: </strong>To investigate and compare the effects of mental fatigue (MF) on biomechanical characteristics associated with non-contact anterior cruciate ligament injury (NC-ACLI) in male college students during stop-jump (SJ) and single-leg landing (SL), and whether it increases NC-ACLI risk.</p><p><strong>Methods: </strong>MF was induced by a 45-min Stroop task and measured using the visual analogue scale for MF (VAS-MF), while motion capture, force platforms, and surface electromyography (SEMG) evaluated biomechanical variables before and after MF induction in thirty-six subjects. Kinematic, kinetic, and SEMG data were analyzed using two-factor repeated measures ANOVA and rank-based nonparametric ANOVA.</p><p><strong>Results: </strong>Following MF induction, VAS-MF scores increased significantly. The ANOVA showed that in both maneuvers, peak vertical ground reaction force increased, while ankle dorsiflexion angle and knee flexion moment decreased. In SJ, knee flexion and internal rotation angles and internal rotation moment decreased, whereas knee abduction moment increased; these parameters did not change significantly in SL. The median frequency of biceps femoris SEMG decreased in SL but remained unchanged in SJ. No significant differences were found in hip flexion angle, knee adduction angle, or SEMG measures of rectus femoris, tibialis anterior, gastrocnemius lateral head, or biceps femoris root mean square.</p><p><strong>Conclusion: </strong>MF partly influences NC-ACLI biomechanics and increases risk in both maneuvers-more pronounced in SJ than in SL-potentially due to MF's impact on central nervous system function, cognition, and attention. MF should be considered in NC-ACLI prevention strategies.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1582873"},"PeriodicalIF":4.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustained capillary enlargement induced by angiogenic gene therapy does not support post-ischemic muscle recovery of hyperlipidemic mice.","authors":"Galina Wirth, Greta Juusola, Hanne Laakso, Nihay Laham-Karam, Seppo Ylä-Herttuala, Petra Korpisalo","doi":"10.3389/fbioe.2025.1512962","DOIUrl":"10.3389/fbioe.2025.1512962","url":null,"abstract":"<p><strong>Background: </strong>Hyperlipidemia is known to impair endothelial function. We have recently shown that hyperlipidemia also blunts native post-ischemic capillary enlargement that is important for efficient skeletal muscle recovery from ischemia as it supports the recovery of arterial driving pressure and through intussusception increases capillary density. The correction of capillary reactivity under hyperlipidemia could, therefore, improve post-ischemic skeletal muscle recovery. This study tested the ability of adenoviral (Ad) vascular endothelial growth factor (VEGF) gene therapy to rescue capillary enlargement and improve post-ischemic muscle repair in hyperlipidemic mice.</p><p><strong>Methods: </strong>AdVEGF or AdLacZ-control vector were delivered into the calf muscles of aged, hyperlipidemic LDLR^-/-ApoB^100/100 mice (n = 58) after induction of acute ischemia. The effects of AdVEGF on capillary phenotype, tissue edema, restoration of blood flow parameters, microvascular hemoglobin oxygenation and tissue damage/regeneration were evaluated using immunohistological analyses, magnetic resonance imaging, contrast-enhanced ultrasound imaging, photoacoustic imaging and histological analyses, respectively, up to 29 days after induced ischemia and gene transfer.</p><p><strong>Results: </strong>It was found that AdVEGF gene therapy was able to promote capillary enlargement (P < 0.05) that led to recovery of arterial driving pressure in ischemic LDLR^-/-ApoB^100/100 muscles. However, capillary enlargement induced by AdVEGF in the hyperlipidemic mice was delayed, had a long-lasting effect (P < 0.05) and did not promote intussusception. Instead, side-effects of VEGF-induced capillary enlargement, i.e., tissue edema (P < 0.01) and subsequently delayed blood flow recovery (P < 0.05), aggravated ischemic tissue damage (P < 0.01).</p><p><strong>Conclusion: </strong>Hyperlipidemia or old age did not seem to impair AdVEGF-induced capillary enlargement. However, regarding the side-effects of capillary enlargement, therapies trying to promote post-ischemic skeletal muscle recovery through angiogenesis should consider not only capillary size or density but also timing and dynamics of the capillary changes.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1512962"},"PeriodicalIF":4.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}