Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Hydrogel and scalp/skin conductivities impact dose from tumor treating fields.","authors":"Edwin Lok, Olivia Liang, Monika Haack, Eric T Wong","doi":"10.3389/fbioe.2025.1484317","DOIUrl":"10.3389/fbioe.2025.1484317","url":null,"abstract":"<p><strong>Purpose: </strong>Tumor Treating Fields (TTFields) are delivered by transducer arrays applied to scalp or body surface for treatment of multiple malignancies. Dermatologic complications are thought to be related to hydrogel situated between the electrodes and scalp or skin to facilitate electric field penetration. High intensity of TTFields on these surfaces may also be a contributing factor. We explored conductivity changes in the hydrogel and skin to improve TTFields coverage and penetration.</p><p><strong>Methods: </strong>Magnetic resonance imaging datasets from 12 glioblastoma patients and attenuation-corrected positron emission tomography-computed tomography datasets from 3 non-small cell lung and 2 ovarian carcinoma patients were used to segment anatomic structures. Finite element mesh models were generated and solved for distribution of applied electric fields, rate of energy deposition, and current density at the gross tumor volume (GTV) and clinical target volume (CTV). Electric field-volume, specific absorption rate-volume, and current density-volume histograms were generated, by which plan quality metrics were used to evaluate relative differences in field coverage between models at various hydrogel and skin conductivities.</p><p><strong>Results: </strong>By varying conductivity of hydrogel, TTFields coverage at GTV or CTV increased up to 0.5 S/m for head and 1.0 S/m for thorax and pelvis models, and no additional increase was observed beyond these saturation points. Although scalp hotspots increased or decreased by +1.5%, -0.1%, and -0.9% in E_5%, SAR_5%, and CD_5%, the skin hotspots increased by as much as +23.5%, +45.7%, and +20.6%, respectively. When altering conductivity of the entire scalp, TTFields coverage peaked near 1 S/m at the GTV or CTV for the head models. TTFields coverage in both the GTV and scalp increased up to 1 S/m for the head models but plateaued thereafter. Contouring under the scalp increased scalp hotspots by +316% in E_5% at 1 S/m compared to altering the conductivity of the entire scalp. GTV hotspots decreased by +17% in E_5% at 1 S/m.</p><p><strong>Conclusion: </strong>TTFields delivery can be modulated by the conductivity of hydrogel and scalp/skin at the transducer-scalp or transducer-skin interface. Optimizing this aspect of TTFields delivery may increase tumor control while minimizing toxicity at the scalp or skin.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1484317"},"PeriodicalIF":4.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"There and turn back again: the application of phage serine integrases in eukaryotic systems.","authors":"Thais Torquato Sales, Marco Antônio de Oliveira, Lilian Hasegawa Florentino, Rayane Nunes Lima, Elibio Rech","doi":"10.3389/fbioe.2025.1478413","DOIUrl":"10.3389/fbioe.2025.1478413","url":null,"abstract":"<p><p>Serine integrases (Ints) have gained prominence and have been extensively used in Synthetic Biology due to their ability to modify DNA sequences. Ints are recombinases encoded by the phage genome and have been used to unidirectionally catalyze an insertion, excision, or inversion of a specific DNA sequence between the two attachment sites (<i>att</i>) <i>attB</i> (bacterial attachment site) and <i>attP</i> (phage attachment site). The entire process is highly specific and accurate; therefore, Ints are widely used in genetic engineering and have been extensively studied due to their unique site-specific recombination properties and potential genome editing applications. Furthermore, new recombinational factors (RDFs) and their determinants are constantly being discovered, underlining the need to update progress in research involving Ints in eukaryotic cells. In this way, this review aims to provide an overview of Ints in eukaryotic cells and highlight how Ints can be used in innovative ways to advance genetic engineering applications in health, agriculture, and environmental sciences.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1478413"},"PeriodicalIF":4.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic pathways to sustainability: review of purple non-sulfur bacteria potential in agri-food waste valorization.","authors":"Guillaume Bayon-Vicente, Laura Toubeau, Manon Gilson, Guillaume Gégo, Nishitha Landgey, Simone Krings, Baptiste Leroy","doi":"10.3389/fbioe.2025.1529032","DOIUrl":"10.3389/fbioe.2025.1529032","url":null,"abstract":"<p><p>Agri-food waste (AFW) represents a significant fraction of the material generated by the agri-food industry, which itself accounts for almost one-third of the annual global anthropogenic greenhouse gas (GHG) emissions. Considering the growing global population and the consequent rise in food demand, the management and valorization of this waste are essential to ensure the sustainability of the entire food chain for future generations. Recycling agri-food waste offers a promising strategy to mitigate the sector's environmental impact, particularly when the waste consists of food-grade materials that enhance its intrinsic value. Retaining such products within the agri-food chain by converting them into feed or food, a process referred to as \"waste upcycling,\" is therefore of critical importance. Purple non-sulfur bacteria (PNSB) are emerging as promising candidates for AFW upcycling due to their remarkable metabolic versatility, which allows them to metabolize a wide range of organic substrates, including carbohydrates, volatile fatty acids (VFAs), and alcohols, into valuable microbial biomass. This biomass is notably rich in superior quality proteins, vitamins, pigments, and other high-value compounds. The phototrophic metabolism of PNSB is particularly advantageous for organic matter valorization, as the carbon conversion yield approaches unity by utilizing light as an energy source. This review explores the potential of PNSB in upcycling AFW streams derived from various sources, such as fruit and vegetable residues, as well as effluents from the dairy, brewery, and sugar industries. The pre-treatment methods required to optimize substrate availability are also discussed. Furthermore, we examine the metabolic pathways utilized by PNSB under phototrophic conditions to assimilate the most common carbon substrates found in AFW, highlighting critical gaps in our understanding of their metabolism. Additionally, challenges and opportunities in AFW valorization, with a focus on PNSB applications, are identified. This review underscores recent advancements and ongoing challenges, emphasizing the potential role of PNSB in driving sustainable circular bioeconomy applications for AFW.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1529032"},"PeriodicalIF":4.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of running shoe stack height on running style and stability during level running at different running speeds.","authors":"Cagla Kettner, Bernd Stetter, Thorsten Stein","doi":"10.3389/fbioe.2025.1526752","DOIUrl":"10.3389/fbioe.2025.1526752","url":null,"abstract":"<p><p>The footwear market contains a wide variety of running shoe solutions aiming at optimizing performance and minimizing injuries. Stack height is one of the most highly discussed design features of running shoes, but its effects are not yet well understood. This study investigated the effects of different shoes differing mainly in their stack heights (High: 50 mm, Medium: 35 mm and Low: 27 mm) on running style and stability during treadmill running at 10 and 15 km/h. A total of 17 healthy experienced runners participated. The kinematic data were recorded with a 3D motion capturing system. The running style was investigated with duty factor (DF) and leg length normalized to step frequency (SF_norm). Additionally, the ratio of landing to take-off duration, the lower body joint angle time series in the sagittal and frontal planes, the vertical center of mass oscillation (COM_osc), and the stiffness parameters (k_ver and k_leg) were compared for different conditions. The stability was analyzed using linear (i.e., discrete frontal ankle parameters) and nonlinear methods (i.e., Maximum Lyapunov Exponent for local dynamic stability of head, trunk, hip, and foot, and detrended fluctuation analysis of stride time). High resulted in longer ground contact relative to stride time (i.e., DF) compared to Low. The higher the stack height, the higher was the COM_osc. Furthermore, High led to a longer foot eversion during stance compared to Medium. In addition, the local dynamic stability of the hip decreased with High in comparison with Low. The higher stack heights (≥35 mm) led to a lower SF_norm at 15 km/h but not at 10 km/h. The remaining shoe effects were independent of running speed. Findings showed that changes in stack height can affect running style. Furthermore, the highest stack height resulted in changes related with instabilities (i.e., longer foot eversion and lower hip dynamic stability) which may be a critical issue in terms of injuries and performance. However, this study did not include joint load analysis or running performance measures such as VO₂. Future studies may benefit from combination of analysis approaches to better understand stack height effects on running injuries and performance.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1526752"},"PeriodicalIF":4.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing biomaterial surface properties to improve orthopedic hip implant safety and function in a Safe-by-Design approach.","authors":"Anniek M C Gielen, Niels M Leijten, Payal P S Balraadjsing, Hedwig M Braakhuis, Hannah Abee, Jacobus J Arts, Annemarie P van Wezel, Agnes G Oomen, Nick R M Beijer","doi":"10.3389/fbioe.2025.1504883","DOIUrl":"10.3389/fbioe.2025.1504883","url":null,"abstract":"<p><p>Orthopedic hip implant failure due to adverse events, such as infection, are still a major problem leading to high morbidity and mortality. Over the years, various innovative biomaterials have been investigated to improve safety and functionality of implants. Although novel biomaterials show initial promising results, many fail at the (later) stages of safety testing. We performed a literature review serving as a first step in a Safe-by-Design (SbD) approach. SbD is a strategy which includes safety considerations at early development stages and that streamlines the pre-clinical safety assessment of innovative medical implants. In a SbD approach, the standard safety assessment of medical implants (e.g., ISO10993) is complemented with insights on cell-biomaterial interactions allowing for a better <i>in vivo</i> response prediction. As a first step, these insights are based on existing information from literature. Therefore, in this review, correlations between implant biomaterial surface properties and key biological processes, relevant for the success and safety of titanium hip implants, are investigated. In particular, the influence of biomaterial roughness, wettability and pore size on key biological processes for a hip implant (osseointegration, bacterial adhesion and the immune response) are examined. Although it was found that no ideal combination of properties exist to satisfy the key biological processes simultaneously, the gathered insights provide directions for the development of safe and functional biomaterials. Altogether, an assessment of the different aspects of safety at early development stages within an SbD approach can improve biomaterial functionality and thus safety.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1504883"},"PeriodicalIF":4.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lower limb biomechanical differences between forehand and backhand forward lunges in amateur female badminton players.","authors":"Zhonghao Xie, Jing Pan, Xingyu Wu, Huiting Liang, Bosi Chen, Dongping Tan, Meng Wu, Zhiguan Huang","doi":"10.3389/fbioe.2025.1558918","DOIUrl":"10.3389/fbioe.2025.1558918","url":null,"abstract":"<p><strong>Background: </strong>Forehand and backhand forward lunges are frequently performed in badminton, placing significant demands on the lower limbs. The purpose of this study was to examine the differences in lower limb biomechanics between these two lunge types in female amateur players.</p><p><strong>Methods: </strong>This study involved 17 female amateur badminton players performing forehand and backhand forward lunges. Lower limb kinematics and dynamics were recorded using an eight-camera Vicon motion capture system and two AMTI force plates. Variables such as joint angle, range of motion, stiffness, and ground reaction forces measured during the stance phase were analyzed using paired t-tests. To account for the one-dimensional nature of joint angles, moments, and ground reaction forces, the analysis was performed using paired sample t-tests in Statistical Parametric Mapping 1D.</p><p><strong>Results: </strong>The forehand lunge exhibited a smaller hip flexion angle, greater hip internal rotation angle, and increased hip stiffness compared to the backhand lunge. The backhand lunge, in contrast, demonstrated a higher ankle varus angle and greater transverse plane hip range of motion. SPM1D analysis revealed significant differences in both the early (0%-10%) and late (80%-100%) phases of the stance phase. In the early phase, the backhand lunge showed a larger internal rotation moment at the hip, an external rotation moment at the knee, and a smaller knee extension moment. In the late phase, the forehand lunge revealed greater internal rotation moments at the hip, external rotation moments at the knee, ankle valgus moments, and smaller knee flexion moments.</p><p><strong>Conclusion: </strong>The backhand lunge requires greater hip internal rotation than the forehand lunge. Additionally, it is associated with higher ankle varus angles, which may increase the risk of ankle injuries. In contrast, the forehand lunge demonstrates greater hip stiffness, potentially reflecting an adaptation of the lower limb to varying directional demands. These findings emphasize the importance of incorporating targeted ankle and hip training exercises into conditioning programs.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1558918"},"PeriodicalIF":4.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical effects of altered multifidus muscle morphology on cervical spine tissues.","authors":"Guangming Xu, Chenxing Li, Zhizhong Sheng","doi":"10.3389/fbioe.2025.1524844","DOIUrl":"10.3389/fbioe.2025.1524844","url":null,"abstract":"<p><strong>Background: </strong>Muscle fat infiltration and atrophy were common pathomorphologic changes in the paravertebral muscles. Some studies indicated that degeneration of paravertebral muscles may be one of the important causes of chronic neck pain. Therefore, we investigated the mechanical effects of multifidus muscle morphologic changes on cervical spine tissues by constructing cervical spine models of multfiidus muscle with different degrees of atrophy.</p><p><strong>Method: </strong>Three-dimensional finite element models of the cervical spine with 100%, 80%, and 50% with the multifidus muscle were constructed by referring to previous literature. According to the mechanical loading conditions in previous literature, the patient's head weight and 1 Nm of loading were considered to be applied to the cervical spine, and the mechanical differences in the cervical intervertebral discs, joint capsule, cartilage endplates and range of motion (ROM) due to the morphological changes of the multifidus muscle were recorded and analyzed.</p><p><strong>Result: </strong>Under anterior flexion loading, model C increasing by 55% and 22% at the C5-6 segment compared to A and B, respectively. Among the three model groups, the stresses in the discs of the lower segments (C4-C7) were significantly higher than those in the upper segments. Under posterior extension loading, the strain values of the joint capsule were higher in the lower cervical segments, with the maximum strain values in the C5-6 segments. The maximum strain values in the lower cartilage endplates were in the C5-6 segments in model group A, whereas the maximum values were in the C4-5 segments in both models B and C. The maximum values in the lower cervical segments were in the C4-6 and C4-5 segments. In addition, a similar trend described above occurs in lateral bending and axial rotation conditions. The ROM of the lower cervical was higher than that of the upper cervical vertebrae, except in lateral bending conditions.</p><p><strong>Conclusion: </strong>In this study, we constructed the morphology of the multifidus muscle to more realistically simulate the mechanical environment of the cervical spine <i>in vivo</i> and quantitatively explored the effects of multifidus muscle atrophy on cervical spine tissues. The results showed that volume atrophy of the multifidus muscle altered the mechanical response of cervical spine tissues. Volume atrophy of the multifidus muscle significantly increased the mechanical indexes of the cervical spine tissues, in which the cervical disc stresses, joint capsule strains, and cartilage endplates increased significantly. Compared with the mechanical changes in the upper cervical segments, the mechanical changes in the lower cervical segments were higher. Therefore, it is important to moderately increase the functional exercise of the multifidus muscle to prevent atrophy leading to abnormal stress concentrations in cervical tissues.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1524844"},"PeriodicalIF":4.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cofactor engineering for improved production of 2,4-dihydroxybutyric acid via the synthetic homoserine pathway.","authors":"Nadine Ihle, Laura Grüßner, Ceren Alkim, T A Stefanie Nguyen, Thomas Walther, Cláudio J R Frazão","doi":"10.3389/fbioe.2025.1504785","DOIUrl":"10.3389/fbioe.2025.1504785","url":null,"abstract":"<p><p>(L)-2,4-dihydroxybutyrate (DHB) is a versatile compound that can serve as a precursor for the synthesis of the methionine analog 2-hydroxy-4-(methylthio)butyrate and new advanced polymers. We previously implemented in <i>Escherichia coli</i> an artificial biosynthetic pathway for the aerobic production of DHB from glucose, which relies on the deamination of (L)-homoserine followed by the reduction of 2-oxo-4-hydroxybutyrate (OHB) and yields DHB by an enzyme-bearing NADH-dependent OHB reductase activity. Under aerobic conditions, using NADPH as a cofactor is more favorable for reduction processes. We report the construction of an NADPH-dependent OHB reductase and increased intracellular NADPH supply by metabolic engineering to improve DHB production. Key cofactor discriminating positions were identified in the previously engineered NADH-dependent OHB reductase (<i>E. coli</i> malate dehydrogenase I12V:R81A:M85Q:D86S:G179D) and tested by mutational scanning. The two point mutations D34G:I35R were found to increase the specificity for NADPH by more than three orders of magnitude. Using the new OHB reductase enzyme, replacing the homoserine transaminase with the improved variant Ec.AlaC A142P:Y275D and increasing the NADPH supply by overexpressing the <i>pntAB</i> gene encoding the membrane-bound transhydrogenase yielded a strain that produced DHB from glucose at a yield of 0.25 mol_DHB mol_Glucose ^-1 in shake-flask experiments, which corresponds to a 50% increase compared to previous producer strains. Upon 24 h of batch cultivation of the most advanced DHB producer strain constructed in this work, a volumetric productivity of 0.83 mmol_DHB L^-1 h^-1 was reached.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1504785"},"PeriodicalIF":4.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between pristine nC₆₀ and bovine serum albumin by fluorimetry: assessment of inner filter effect corrections.","authors":"Yu Zhang, Ye Zheng, Yuanjie Li, Shufang Liu","doi":"10.3389/fbioe.2025.1518698","DOIUrl":"10.3389/fbioe.2025.1518698","url":null,"abstract":"<p><strong>Introduction: </strong>Fluorescence spectrometry is widely used to investigate nanomaterial-protein interactions, a crucial component of nanomaterial safety evaluation. However, the inner filter effect (IFE) significantly distorts fluorescence data during the analysis of fullerene (nC₆₀) -protein interactions. Systematic correction methods for this system are rarely reported.</p><p><strong>Methods: </strong>In this study, bovine serum albumin (BSA) served as the protein model, four mathematical formulas (Lakowicz, Gauthier, Tucker, and Chen models) were comparatively evaluated for IFE correction in fluorescence analysis. The correction results were compared to propose an optimal correction method for the interaction between nC60 and BSA. Binding parameters were calculated from corrected data, and quenching mechanisms were analyzed using Stern-Volmer equations.</p><p><strong>Results: </strong>At room temperature with low nC₆₀ concentrations (<2.0 × 10^-5 mol/L), Chen's model demonstrated optimal IFE correction accuracy. Corrected data indicated static quenching between nC₆₀ and BSA, with a binding constant of K = 2.95 × 10⁹ L/mol and approximately two binding sites.</p><p><strong>Discussion: </strong>This study offers methodological guidance for IFE correction and accurate fluorescence analysis in the investigation of interactions between nanomaterials and biomolecules. Thus, it provides a reliable analytical method for the bio-safety assessment of nanomaterials.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1518698"},"PeriodicalIF":4.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-temperature cold plasma promotes wound healing by inhibiting skin inflammation and improving skin microbiome.","authors":"Jie Zhou, Zengkun Sun, Xiaoru Wang, Shouguo Wang, Wen Jiang, Dongqi Tang, Tao Xia, Fang Xiao","doi":"10.3389/fbioe.2025.1511259","DOIUrl":"10.3389/fbioe.2025.1511259","url":null,"abstract":"<p><p>Wound healing includes four consecutive and overlapping stages of hemostasis, inflammation, proliferation, and remodeling. Factors such as aging, infection, and chronic diseases can lead to chronic wounds and delayed healing. Low-temperature cold plasma (LTCP) is an emerging physical therapy for wound healing, characterized by its safety, environmental friendliness, and ease of operation. This study utilized a self-developed LTCP device to investigate its biological effects and mechanisms on wound healing in adult and elderly mice. Histopathological studies found that LTCP significantly accelerated the healing rate of skin wounds in mice, with particularly pronounced effects in elderly mice. LTCP can markedly inhibit the expression of pro-inflammatory cytokines (<i>TNF-α</i>, <i>IL-6</i>, <i>IL-1β</i>) and senescence-associated secretory phenotype factors (<i>MMP-3</i>, <i>MMP-9</i>), while significantly increasing the expression of tissue repair-related factors, such as <i>VEGF</i>, <i>bFGF</i>, <i>TGF-β</i>, <i>COL-I</i>, and <i>α-SMA</i>. It also regulated the expression of genes related to cell proliferation and migration (<i>Aqp5</i>, <i>Spint1</i>), inflammation response (<i>Nlrp3</i>, <i>Icam1</i>), and angiogenesis (<i>Ptx3</i>, <i>Thbs1</i>), promoting cell proliferation and inhibit apoptosis. Furthermore, LTCP treatment reduced the relative abundance of harmful bacteria such as <i>Delftia</i>, <i>Stenotrophomonas</i>, <i>Enterococcus</i>, and <i>Enterobacter</i> in skin wounds, while increasing the relative abundance of beneficial bacteria such as <i>Muribaculaceae</i>, <i>Acinetobacter</i>, <i>Lachnospiraceae NK4A136_group</i>, and <i>un_f__Lachnospiraceae</i>, thereby improving the microbial community structure of skin wounds. These research findings are of significant implications for understanding the mechanism of skin wound healing, as well as for the treatment and clinical applications of skin wounds, especially aging skin.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1511259"},"PeriodicalIF":4.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}