Frontiers in Bioengineering and Biotechnology最新文献

{"title":"FixThePig: a custom 3D-printed femoral intramedullary nailing for preclinical research applications.","authors":"Julie Manon, Alexandre Englebert, Robin Evrard, Thomas Schubert, Olivier Cornu","doi":"10.3389/fbioe.2024.1478676","DOIUrl":"10.3389/fbioe.2024.1478676","url":null,"abstract":"<p><strong>Background: </strong>Critical-size bone defects (CSBDs) pose significant challenges in clinical orthopaedics and traumatology. Developing reliable preclinical models that accurately simulate human conditions is crucial for translational research. This study addresses the need for a reliable preclinical model by evaluating the design and efficacy of a custom-made 3D-printed intramedullary nail (IMN) specifically for CSBDs in minipigs. The study aims to answer the following questions: Can a custom-made 3D-printed IMN be designed for femoral osteosynthesis in minipigs? Does the use of the custom-made IMN result in consistent and reproducible surgical procedure, particularly in the creation and fixation of CSBDs? Can the custom-made IMN effectively treat and promote bone consolidation of CSBDs?</p><p><strong>Hypothesis: </strong>The custom-made 3D-printed IMN can be designed to effectively create, fix and treat CSBDs in minipigs, resulting in consistent surgical outcomes.</p><p><strong>Materials and methods: </strong>The IMN was designed based on CT scans of minipig femurs, considering factors such as femoral curvature, length, and medullary canal diameters. It was 3D-printed in titanium and evaluated through both <i>in vitro</i> and <i>in vivo</i> testing. Female Aachen minipigs underwent bilateral femoral surgeries to create and fix CSBDs using the custom-made IMN. Post-operative follow-up included X-rays and CT scans every 2 weeks, with manual examination of explanted femurs to assess consolidation and mechanical stability after 3 months.</p><p><strong>Results: </strong>The custom-made IMN effectively fitted the minipig femoral anatomy and facilitated reproducible surgical outcomes. Symmetric double osteotomies were successfully performed, and allografts showed minimal morphological discrepancies. However, proximal fixation faced challenges, leading to non-union in several cases, while most distal osteotomy sites achieved stable consolidation.</p><p><strong>Discussion: </strong>The custom-made 3D-printed IMN demonstrated potential in modelling and treating CSBDs in minipigs. While the design effectively supported distal bone healing, issues with proximal fixation highlight the need for further refinements. Potential improvements include better screw placement, additional mechanical support, and adaptations such as a reduction clamp or a cephalic screw to enhance stability and distribute forces more effectively.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1478676"},"PeriodicalIF":4.3,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current advances in the development of microRNA-integrated tissue engineering strategies: a cornerstone of regenerative medicine.","authors":"Luis Germán Castañón-Cortés, Luis Alberto Bravo-Vázquez, Grecia Santoyo-Valencia, Sara Medina-Feria, Padmavati Sahare, Asim K Duttaroy, Sujay Paul","doi":"10.3389/fbioe.2024.1484151","DOIUrl":"10.3389/fbioe.2024.1484151","url":null,"abstract":"<p><p>Regenerative medicine is an innovative scientific field focused on repairing, replacing, or regenerating damaged tissues and organs to restore their normal functions. A central aspect of this research arena relies on the use of tissue-engineered scaffolds, which serve as structural supports that mimic the extracellular matrix, providing an environment that orchestrates cell growth and tissue formation. Remarkably, the therapeutic efficacy of these scaffolds can be improved by harnessing the properties of other molecules or compounds that have crucial roles in healing and regeneration pathways, such as phytochemicals, enzymes, transcription factors, and non-coding RNAs (ncRNAs). In particular, microRNAs (miRNAs) are a class of tiny (20-24 nt), highly conserved ncRNAs that play a critical role in the regulation of gene expression at the post-transcriptional level. Accordingly, miRNAs are involved in a myriad of biological processes, including cell differentiation, proliferation, and apoptosis, as well as tissue regeneration, angiogenesis, and osteogenesis. On this basis, over the past years, a number of research studies have demonstrated that miRNAs can be integrated into tissue-engineered scaffolds to create advanced therapeutic platforms that precisely modulate cellular behavior and offer a controlled and targeted release of miRNAs to optimize tissue repair and regeneration. Therefore, in this current review, we discuss the most recent advances in the development of miRNA-loaded tissue-engineered scaffolds and provide an overview of the future outlooks that should be aborded in this area of study in order to lay the groundwork for the clinical translation of these tissue engineering approaches.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1484151"},"PeriodicalIF":4.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-Cas systems and applications for crop bioengineering.","authors":"Mireia Uranga, Ana Montserrat Martín-Hernández, Nico De Storme, Fabio Pasin","doi":"10.3389/fbioe.2024.1483857","DOIUrl":"10.3389/fbioe.2024.1483857","url":null,"abstract":"<p><p>CRISPR-Cas technologies contribute to enhancing our understanding of plant gene functions, and to the precise breeding of crop traits. Here, we review the latest progress in plant genome editing, focusing on emerging CRISPR-Cas systems, DNA-free delivery methods, and advanced editing approaches. By illustrating CRISPR-Cas applications for improving crop performance and food quality, we highlight the potential of genome-edited crops to contribute to sustainable agriculture and food security.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1483857"},"PeriodicalIF":4.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective and new technologies in kidney tissue engineering.","authors":"Hossein Rayat Pisheh, Mobin Haghdel, Mahboube Jahangir, Monireh Sadat Hoseinian, Shaghayegh Rostami Yasuj, Ali Sarhadi Roodbari","doi":"10.3389/fbioe.2024.1476510","DOIUrl":"10.3389/fbioe.2024.1476510","url":null,"abstract":"<p><p>Kidney disease encompasses a wide spectrum of conditions, ranging from simple infections to chronic kidney disease. When the kidneys are unable to filter blood and remove waste products, these abnormalities can lead to kidney failure. In severe cases of kidney failure, kidney transplantation is considered the only definitive treatment. Worldwide, the World Health Organization (WHO) repeatedly emphasizes the importance of organ donation and increasing transplantation rates. Many countries implement national programs to promote the culture of organ donation and improve patient access to kidney transplantation. The extent to which this procedure is performed varies across countries and is influenced by several factors, including the volume of organ donation, medical infrastructure, access to technology and health policies. However, a kidney transplant comes with challenges and problems that impact its success. Kidney tissue engineering is a new approach that shows promise for repairing and replacing damaged kidney tissue. This article reviews recent advances in kidney tissue engineering, focusing on engineered structures such as hydrogels, electrospinning, 3D bioprinting, and microfluidic systems. By mimicking the extracellular environment of the kidney, these structures provide suitable conditions for the growth and development of kidney cells. The role of these structures in the formation of blood vessels, the mimicry of kidney functions and the challenges in this field were also discussed. The results of this study show that kidney tissue engineering has high potential for treating kidney diseases and reducing the need for kidney transplantation. However, to achieve clinical application of this technology, further research is required to improve the biocompatibility, vascularization and long-term performance of engineered tissues.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1476510"},"PeriodicalIF":4.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions in <i>Aspergillus niger</i>.","authors":"Guoliang Yuan, Shuang Deng, Jeffrey J Czajka, Ziyu Dai, Beth A Hofstad, Joonhoon Kim, Kyle R Pomraning","doi":"10.3389/fbioe.2024.1452496","DOIUrl":"10.3389/fbioe.2024.1452496","url":null,"abstract":"<p><p>CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas systems, Cas9 and Cas12a are widely used in genetic and metabolic engineering. In filamentous fungi, both Cas9 and Cas12a have been utilized as CRISPR nucleases. In this work we first compared efficacies and types of genetic edits for CRISPR-Cas9 and -Cas12a systems at the polyketide synthase (<i>albA</i>) gene locus in <i>Aspergillus niger</i>. By employing a tRNA-based gRNA polycistronic cassette, both Cas9 and Cas12a have demonstrated equally remarkable editing efficacy. Cas12a showed potential superiority over Cas9 protein when one gRNA was used for targeting, achieving an editing efficiency of 86.5% compared to 31.7% for Cas9. Moreover, when employing two gRNAs for targeting, both systems achieved up to 100% editing efficiency for single gene editing. In addition, the CRISPR-Cas9 system has been reported to induce large genomic deletions in various species. However, its use for engineering large chromosomal segments deletions in filamentous fungi still requires optimization. Here, we engineered Cas9 and -Cas12a-induced large genomic fragment deletions by targeting various genomic regions of <i>A</i>. <i>niger</i> ranging from 3.5 kb to 40 kb. Our findings demonstrate that targeted engineering of large chromosomal segments can be achieved, with deletions of up to 69.1% efficiency. Furthermore, by targeting a secondary metabolite gene cluster, we show that fragments over 100 kb can be efficiently and specifically deleted using the CRISPR-Cas9 or -Cas12a system. Overall, in this paper, we present an efficient multi-gRNA genome editing system utilizing Cas9 or Cas12a that enables highly efficient targeted editing of genes and large chromosomal regions in <i>A</i>. <i>niger</i>.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1452496"},"PeriodicalIF":4.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Human digital twins for medical and product engineering.","authors":"Jörg Miehling, Julie Choisne, Anne D Koelewijn","doi":"10.3389/fbioe.2024.1489975","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1489975","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1489975"},"PeriodicalIF":4.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Novel computational fluid dynamics methods for diagnosis, monitoring, prediction, and personalized treatment for cardiovascular disease and cancer metastasis.","authors":"Zahra Keshavarz Motamed, Nima Maftoon, Lakshmi Prasad Dasi, John F LaDisa","doi":"10.3389/fbioe.2024.1491950","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1491950","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1491950"},"PeriodicalIF":4.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the precision of continuum robots in orthopedic surgery based on mechanical principles.","authors":"Tongtao Pang, Jinkui Liang, Zechen Lin, Xubin Zhang, Finxin Du","doi":"10.3389/fbioe.2024.1470069","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1470069","url":null,"abstract":"<p><strong>Introduction: </strong>In the field of orthopedic surgery, the notched continuum robot has garnered significant attention due to its passive compliance, making it particularly suitable for procedures in complex and delicate bone and joint regions. However, accurately modeling the notched continuum robot remains a significant challenge.</p><p><strong>Methods: </strong>This paper proposes a high-precision mechanical modeling method for the notched continuum robot to address this issue. The flexible beam deflection prediction model based on the beam constraint model is established. The force balance friction model considering internal friction is established. An accurate static model is obtained, which can accurately estimate the deformation and deflection behavior of the robot according to the input driving force. The kinematic model of the notched continuum robot based on the static model is established. This method achieves high accuracywhile ensuring computational efficiency.</p><p><strong>Results: </strong>Experimental results demonstrate that the static model's error is only 0.1629 mm, which corresponds to 0.25% of the total length of the continuum robot, which is 66 mm.</p><p><strong>Discussion: </strong>This research provides valuable insights into the modeling and control of continuum robots and holds significant implications for advancing precision in orthopedic surgery.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1470069"},"PeriodicalIF":4.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of human cornea finite element modeling: geometry modeling, constitutive modeling, and outlooks.","authors":"Guobao Pang, Chenyan Wang, Xiaojun Wang, Xiaona Li, Qiaoyu Meng","doi":"10.3389/fbioe.2024.1455027","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1455027","url":null,"abstract":"<p><p>The cornea is a vital tissue of the human body. The health status of the cornea has a great impact on the quality life of person. There has been a great deal of research on the human cornea biomechancis. However, the difficulty in obtaining the human cornea has greatly limited the research of cornea biomechancis. Using finite element modelling has become a very effective and economical means for studying mechanical properties of human cornea. In this review, the geometrical and constitutive models of the cornea are summarised and analysed, respectively. Some factors affecting of the finite element calculation are discussed. In addition, prospects and challenges for the finite element model of the human cornea are presented. This review will be helpful to researchers performing studies in the relevant fields of human cornea finite element analysis.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1455027"},"PeriodicalIF":4.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142550051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of anterior knee displacement on knee joint load during the forward bow step in Tai Chi.","authors":"Lijun Hua, Gengchao Bi, Yanlong Zhang, Kai Wang, Jiao Liu","doi":"10.3389/fbioe.2024.1458737","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1458737","url":null,"abstract":"<p><strong>Background: </strong>While the forward bow step is a crucial component of Tai Chi (TC) practice, little research has been conducted on its impact on knee joint load and muscle coordination. This study aims to investigate the effects of three different knee forward positions during the TC forward bow step on knee joint loading.</p><p><strong>Methods: </strong>Twenty TC practitioners were recruited, and motion capture systems, force platforms, and surface electromyography were utilized to synchronously collect biomechanical parameters of three types of forward bow steps: knee joint not exceeding the tip of the foot (NETT), knee joint forward movement level with the tip of the foot (LTT), and knee joint forward movement exceeding the tip of the foot (ETT). Ligament and muscle forces were calculated using OpenSim software for musculoskeletal modeling and simulation. One-way ANOVA was used to analyze the variations of the indicators during the peak anterior displacement of the knee joint in three movements. Additionally, spm1d one-way ANOVA was employed to examine the variations in the one-dimensional curve of the indicators throughout the entire movement process.</p><p><strong>Results: </strong>Compared with LTT and ETT, the NETT posture was associated with significantly decreased knee flexion angle (F = 27.445, <i>p</i> = 0.001), knee anterior-posterior translation (F = 36.07, <i>p</i> < 0.001), flexion-extension torque (F = 22.232, <i>p</i> = 0.001), ligament force (F = 9.055, <i>p</i> = 0.011). Additionally, there was also a significant reduction in muscle strength, including quadriceps (F = 62.9, <i>p</i> < 0.001), long biceps femoris (F = 18.631, <i>p</i> = 0.002), lateral gastrocnemius (F = 24.933, <i>p</i> = 0.001) and soleus (F = 7.637, <i>p</i> = 0.017).</p><p><strong>Conclusion: </strong>This study further confirms that in the forward lunge movement of Tai Chi, the knee joint load is mainly concentrated during the forward movement phase. Compared to the knee joint load at the NETT position, the load is greater at the LTT position; and compared to the LTT position, the load is even greater at the ETT position.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1458737"},"PeriodicalIF":4.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}