Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Comparing sparse inertial sensor setups for sagittal-plane walking and running reconstructions.","authors":"Eva Dorschky, Marlies Nitschke, Matthias Mayer, Ive Weygers, Heiko Gassner, Thomas Seel, Bjoern M Eskofier, Anne D Koelewijn","doi":"10.3389/fbioe.2025.1507162","DOIUrl":"10.3389/fbioe.2025.1507162","url":null,"abstract":"<p><p>Estimating spatiotemporal, kinematic, and kinetic movement variables with little obtrusion to the user is critical for clinical and sports applications. One possible approach is using a sparse inertial sensor setup, where sensors are not placed on all relevant body segments. Here, we investigated if movement variables can be estimated similarly accurate from sparse sensor setups as from a full lower-body sensor setup. We estimated the variables by solving optimal control problems with sagittal plane lower-body musculoskeletal models, in which we minimized an objective that combined tracking of accelerometer and gyroscope data with minimizing muscular effort. We created simulations for 10 participants at three walking and three running speeds, using seven sensor setups with between two and seven sensors located at the feet, shank, thighs, and/or pelvis. We found that differences between variables estimated from inertial sensors and those from optical motion capture were small for all sensor setups. Including all sensors did not necessarily lead to the smallest root mean square deviations (RMSDs) and highest coefficients of determination ( <math> <mrow> <msup><mrow><mtext>R</mtext></mrow> <mrow><mn>2</mn></mrow> </msup> </mrow> </math> ). Setups without a pelvis sensor led to too much forward trunk lean and inaccurate spatiotemporal variables. Mean RMSDs were highest for the setup with two foot-worn inertial sensors (largest error in knee angle during running: 18 deg vs. 11 deg for the full lower-body setup), and ranged between 4.8-18 deg for the joint angles, between 1.0-5.4 BW BH% for the joint moments, and between 0.03 BW-0.49 BW for the ground reaction forces. We found strong or moderate relationships ( <math> <mrow> <msup><mrow><mtext>R</mtext></mrow> <mrow><mn>2</mn></mrow> </msup> <mo>></mo> <mn>0.5</mn></mrow> </math> ) on average for all kinematic and kinetic variables, except for the hip and knee moment for five out of the seven setups. The large range of the coefficient of determination for most kinetic variables indicated individual differences in simulation quality. Therefore, we conclude that we can perform a comprehensive sagittal-plane motion analysis with sparse sensor setups as accurately as with a full sensor setup with sensors on the feet and on either the pelvis or the thighs. Such a sparse sensor setup enables comprehensive movement analysis outside the laboratory, by increasing usability of inertial sensors.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1507162"},"PeriodicalIF":4.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in extracellular vesicles biomanufacturing: a comprehensive overview of large-scale production and clinical research.","authors":"Ziqian Li, Junyu Yan, Xiang Li, Hui Chen, Chen Lin, Yuhang Zhang, Tian Gao, Yabo Zhang, Yue Shu, Shuyuan Pan, Yuntao Zhang","doi":"10.3389/fbioe.2025.1487627","DOIUrl":"10.3389/fbioe.2025.1487627","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are nano-sized, membranous structures secreted by cells into the extracellular space, have attracted considerable attention in the field of biosciences for their role in intercellular communication in various physiological and pathological processes. Their ubiquitous presence in bodily fluids and cell-specific characteristics make them promising candidates as biomarkers. Additionally, their ability to transport biological therapeutics across different biological barriers to specific target cells underscores their significant translational potential for diagnostic and therapeutic purposes. Significant progress has been achieved in the translation of EVs research to clinical applications, however, challenges persist in the large-scale production of EVs, particularly in the areas of scalable manufacturing, efficient isolation methods, drug loading techniques, and advanced characterization technology. This review critically examines the complex processes involved in EVs biogenesis and explores recent developments in large-scale EVs production. By synthesizing knowledge from these fields, this review aims to provide a holistic perspective on the evolving landscape of EVs research and its applications, underscoring both the accomplishments and the obstacles that lie ahead in fully realizing the potential of EVs in biomedicine.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1487627"},"PeriodicalIF":4.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Recent advances in gold nanostructure-based biosensors in detecting diabetes biomarkers.","authors":"Tahereh Jamshidnejad-Tosaramandani, Soheila Kashanian, Kobra Omidfar, Helgi B Schiöth","doi":"10.3389/fbioe.2025.1526911","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1526911","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fbioe.2024.1446355.].</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1526911"},"PeriodicalIF":4.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical impact of cortical bone vs. traditional pedicle screw trajectories: a finite element study on lumbar spinal instrumentation.","authors":"Xishan Li, Khaled H A Abdel-Latif, Jefrem Schwab, Xiang Zhou, Jie Yang, Zully M Ritter, Arndt F Schilling, Maximilian Reinhold","doi":"10.3389/fbioe.2025.1541114","DOIUrl":"10.3389/fbioe.2025.1541114","url":null,"abstract":"<p><strong>Background: </strong>Pedicle screw fixation using the cortical bone trajectory (CBT) enhances stability by engaging cortical bone, offering a valuable alternative to the traditional pedicle screw trajectory (TT). This study used finite element analysis to compare L4-5 instrumentation with CBT and TT screws, investigating whether the increased cortical bone engagement in CBT improves stability but makes it more susceptible to fatigue failure.</p><p><strong>Methods: </strong>A L3-sacrum model was generated using anonymized CT patient data, validated against existing studies, showing consistent ROM (range of motion) values. A mono-segmental L4-5 instrumentation with an interbody fusion cage was configured with both TT and CBT models, differentiated for healthy and osteoporotic bone (reduced Young's modulus). Both models were exposed to simulated biomechanical loading conditions (compression, flexion, extension, lateral bending, and rotation) to calculate screw loosening and breakage risk. Screw loosening was assessed by measuring micro-movements within the screw hole, while screw breakage was evaluated based on maximum stress values and their frequency at the same locations.</p><p><strong>Results: </strong>In both healthy and osteoporotic bone, the CBT model exhibited smaller micro-movements compared to the TT model across all motions. For maximum stress in healthy bone, CBT showed lower stress during right rotation but higher stress in the other six motions. In osteoporotic bone, CBT stress exceeded TT stress in all conditions. The TT model in healthy bone showed stress concentrations at three locations, while CBT distributed stress across five sites. In osteoporotic bone, CBT showed stress at three locations, while TT distributed stress at four. Notably, in the TT model, maximum stress occurred at the screw head in six of seven movements, whereas in the CBT model, three movements showed maximum stress at the screw head and three at the screw tail.</p><p><strong>Conclusion: </strong>CBT screws, by traversing three cortical layers, achieve greater integration with the vertebral bone compared to TT screws, thus reducing the risk of screw loosening. Although this increases the maximum stress on the screws, the stress is more evenly distributed, with the screw tail helping to reduce the risk of breakage.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1541114"},"PeriodicalIF":4.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876142/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent applications of stimulus-responsive smart hydrogels for osteoarthritis therapy.","authors":"Zhuoming Xu, Jintao Liu, Hanyin Hu, Jun Ma, Haiyang Yang, Jiayi Chen, Hongwei Xu, Haodong Hu, Huanhuan Luo, Gang Chen","doi":"10.3389/fbioe.2025.1539566","DOIUrl":"10.3389/fbioe.2025.1539566","url":null,"abstract":"<p><p>Osteoarthritis is one of the most common degenerative joint diseases, which seriously affects the life of middle-aged and elderly people. Traditional treatments such as surgical treatment and systemic medication, often do not achieve the expected or optimal results, which leads to severe trauma and a variety of side effects. Therefore, there is an urgent need to develop novel therapeutic options to overcome these problems. Hydrogels are widely used in biomedical tissue repairing as a platform for loading drugs, proteins and stem cells. In recent years, smart-responsive hydrogels have achieved excellent results as novel drug delivery systems in the treatment of osteoarthritis. This review focuses on the recent advances of endogenous stimuli (including enzymes, pH, reactive oxygen species and temperature, etc.) responsive hydrogels and exogenous stimuli (including light, shear, ultrasound and magnetism, etc.) responsive hydrogels in osteoarthritis treatment. Finally, the current limitations of application and future prospects of smart responsive hydrogels are summarized.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1539566"},"PeriodicalIF":4.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced biomaterials in pressure ulcer prevention and care: from basic research to clinical practice.","authors":"Shaoqiang Tian, Wei Bian","doi":"10.3389/fbioe.2025.1535588","DOIUrl":"10.3389/fbioe.2025.1535588","url":null,"abstract":"<p><p>Pressure ulcers are a common and serious medical condition. Conventional treatment methods often fall short in addressing the complexities of prevention and care. This paper provides a comprehensive review of recent advancements in advanced biomaterials for pressure ulcer management, emphasizing their potential to overcome these limitations. The study highlights the roles of biomaterials in enhancing wound healing, preventing infections, and accelerating recovery. Specific focus is placed on the innovation and application of multi-functional composite materials, intelligent systems, and personalized solutions. Future research should prioritize interdisciplinary collaboration to facilitate the clinical translation of these materials, providing more effective and tailored treatment approaches. These advancements aim to improve the quality of life and health outcomes for patients by offering more reliable, efficient, and patient-specific therapeutic options.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1535588"},"PeriodicalIF":4.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intensification of Vero cell adherence to microcarrier particles during cultivation in a wave bioreactor.","authors":"Z K Mazhed, V E Vasilenko, A A Siniugina, K V Kaa, A S Motov, K O Pokidova, Y Y Ivin, A N Piniaeva, Y K Khapchaev, K A Chernov, A A Ishmukhametov","doi":"10.3389/fbioe.2025.1542060","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1542060","url":null,"abstract":"<p><p>Vaccination is the most effective strategy for fighting viral diseases, with both live and inactivated vaccines remaining crucial despite advancements in subunit vaccine technologies. A key player in vaccine production is the Vero cell line, derived from the kidney cells of the African green monkey, which is essential for manufacturing vaccines against diseases like polio, rabies, yellow fever, and COVID-19. The efficiency of Vero cell cultivation directly impacts vaccine production, often utilizing bioreactors ranging from small (1-10 L) to large (up to several thousand liters). Wave-type bioreactors are commonly employed for initial cell propagation due to their simplicity. However, achieving uniform cell distribution on microcarriers in these systems poses challenges. This study aims to evaluate intermittent stirring during the early cultivation stages to enhance Vero cell distribution and growth, potentially improving overall cultivation efficiency.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1542060"},"PeriodicalIF":4.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Recent advances of additively manufactured noninvasive kinematic biosensors.","authors":"Jeonghoon Lee, Sangmin Park, Jaehoon Lee, Namjung Kim, Min Ku Kim","doi":"10.3389/fbioe.2025.1560319","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1560319","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fbioe.2023.1303004.].</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1560319"},"PeriodicalIF":4.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinting for the construction of drug testing models-development strategies and regulatory concerns.","authors":"Divya Mallya, Mrunmayi Ashish Gadre, S Varadharajan, Kirthanashri S Vasanthan","doi":"10.3389/fbioe.2025.1457872","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1457872","url":null,"abstract":"<p><p>A drug to be successfully launched in the market requires a significant amount of capital, resources and time, where the unsuccessful results in the last stages lead to catastrophic failure for discovering drugs. This is the very reason which calls for the invention of innovative models that can closely mimic the human <i>in vivo</i> model for producing reliable results. Throughout the innovation line, there has been improvement in the rationale <i>in silico</i> designing but yet there is requirement for <i>in vitro-in vivo</i> correlations. During the evolving of the drug testing models, the 3D models produced by different methods have been proven to produce better results than the traditional 2D models. However, the <i>in vitro</i> fabrications of live tissues are still bottleneck in realizing their complete potential. There is an urgent need for the development of single, standard and simplified <i>in vitro</i> 3D tissue models that can be reliable for investigating the biological and pathological aspects of drug discovery, which is yet to be achieved. The existing pre-clinical models have considerable drawbacks despite being the gold standard in pre-clinical research. The major drawback being the interspecies differences and low reliability on the generated results. This gap could be overcome by the fabrication of bioengineered human disease models for drug screening. The advancement in the fabrication of 3D models will provide a valuable tool in screening drugs at different stages as they are one step closer to bio-mimic human tissues. In this review, we have discussed on the evolution of preclinical studies, and different models, including mini tissues, spheroids, organoids, bioengineered three dimensional models and organs on chips. Furthermore, we provide details of different disease models fabricated across various organs and their applications. In addition to this, the review also focuses on the limitations and the current prospects of the role of three dimensionally bioprinted models in drug screening and development.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1457872"},"PeriodicalIF":4.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A chronological history of heart valve prostheses to offer perspectives of their limitations.","authors":"Raquel Ahnert Aguiar Evangelista, Ana Luiza Resende Pires, Breno Valentim Nogueira","doi":"10.3389/fbioe.2025.1533421","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1533421","url":null,"abstract":"<p><p>Prosthetic heart valves (PHV) have been studied for around 70 years. They are the best alternative to save the life of patients with cardiac valve diseases. However, current PHVs may still cause significant disadvantages to patients. In general, native heart valves show complex structures and reproducing their functions challenges scientists. Valve repair and replacement are the options to heal heart valve diseases (VHDs), such as stenosis and regurgitation, which show high morbidity and mortality worldwide. Valve repair contributes to the performance of cardiac cycles. However, it fails to restore valve anatomy to its normal condition. On the other hand, replacement is the only alternative to treat valve degeneration. It may do so by mechanical or bioprosthetic valves. Although prostheses may restructure patients' cardiac cycle, both prostheses may show limitations and potential disadvantages, such as mechanical valves causing thrombogenicity or bioprosthetic valves, calcification. Thus, prostheses require constant improvements to remedy these limitations. Although the design of mechanical valve structures has improved, their raw materials cause great disadvantages, and alternatives for this problem remain scarce. Cardiac valve tissue engineering emerged 30 years ago and has improved over time, e.g., xenografts and fabricated heart valves serving as scaffolds for cell seeding. Thus, this review describes cardiac valve substitutes, starting with the history of valvular prosthesis transplants and ending with some perspectives to alleviate the limitations of artificial valves.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1533421"},"PeriodicalIF":4.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}