Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Mesenchymal stem cells derived extracellular vesicles for chronic kidney disease: pleiotropic mechanisms of actions of a versatile therapy.","authors":"Elena Ceccotti, Marco Quaglia, Giovanni Camussi, Stefania Bruno","doi":"10.3389/fbioe.2025.1612193","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1612193","url":null,"abstract":"<p><p>Chronic kidney disease (CKD) has increasingly become a major health concern worldwide, globally affecting 10%-15% of adults, with significant implications for morbidity and mortality. This progressive condition can potentially evolve into end-stage renal disease (ESRD), requiring dialysis or renal transplant. However, the heaviest impact of CKD is due to an associated increased cardiovascular risk, due to frequently coexisting hypertension and diabetes and non-traditional risk factors, including accumulation of atherogenic toxins, alteration of calcium-phosphate balance, oxidative stress and chronic microinflammation. Mesenchymal stem cells (MSCs) have been proposed as a therapy for CKD due to their immunomodulating and tissue repairing properties. It has been proposed that extracellular vesicles (EVs) may mediate the therapeutic effects of the cells of origin and MSC-EVs have shown promise as treatment of different aspects of CKD in experimental settings. Their anti-fibrotic and anti-apoptotic properties may inhibit progression of CKD and promote healing of tubular and glomerular damage. MSC-EVs can prevent epithelial-mesenchymal transition, a key mechanism of evolution of acute kidney injury towards CKD. These actions may inhibit development of interstitial fibrosis and accumulation of the extracellular matrix components (ECM), key lesions which promote the progression of CKD. Furthermore, MSC-EVs also exert anti-inflammatory and anti-oxidant properties which may reduce vascular damage and cardiovascular risk associated with CKD. For example, Human Liver Stem Cell (HLSC)-derived EVs (HLSC-EVs) can reverse renal and cardiac alterations. As shown in a murine model of partial nephrectomy, HLSC-EVs shuttled proteases with ECM remodeling activity, lending support to the possibility of a simultaneous cardio-nephroprotective effect. Adipose, umbilical cord and inducible- MSCs are other possible sources of EVs potentially applicable to obtain reparative processes in CKD and ESRD. Overall, building experimental evidence suggests that MSC-EVs derived from different sources are a promising therapeutic tool to prevent development and progression of CKD and to reduce related cardiovascular risk. The strength of this therapy lies in its multi-level and pleiotropic actions which appear to interfere with many key etiopathogenetic mechanisms of CKD. Interesting future perspective is a combined therapy associating MSC-EVs with drugs to achieve synergistic effects and recent finding indicate the feasibility of this approach.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1612193"},"PeriodicalIF":4.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on static seating comfort of the Chinese population under different seat angle design parameters.","authors":"Lingbo Yan, Lingchun Yu, Na Li, Luozhe Shangguan, Muni Luo","doi":"10.3389/fbioe.2025.1592166","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1592166","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to deepen the understanding of seating comfort for the Chinese population by developing a human finite element (FE) model.</p><p><strong>Methods: </strong>This model was integrated with a specific vehicle seat FE model to construct a comprehensive human-seat FE model, and the mechanical responses of the human body were analyzed under varying seat angles. Body pressure distribution, intervertebral disc stress and strain, and vertebral body stress were examined to study the relationship between the internal reactions of the human body and surface contact conditions.</p><p><strong>Results: </strong>The results indicate that when the seat is flipped, the trends of disc stress, average pressure, and contact area are consistent, and the maximum strain closely aligns with the maximum pressure. When the backrest is adjusted, lumbar spine stress and surface pressure exhibit similar trends, while disc stress, strain, and the 1-SPD value show consistent patterns.</p><p><strong>Discussion: </strong>The study concludes that increasing the backrest angle does not necessarily enhance comfort. Moreover, the stress variations in the thoracic and lumbar spines correlate with spinal angle alterations, suggesting that spinal angle can serve as a reliable indicator of stress conditions. Finally, the study highlights the correlation between spinal force and body pressure distribution, underscoring the utility of body pressure distribution metrics as a valuable proxy for understanding spinal responses.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1592166"},"PeriodicalIF":4.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-controllable carbon monoxide nano-delivery systems for combined sonodynamic/gaseous therapies.","authors":"Chong Feng, Shuang Song, Xiaoyu Zhang, Jing Wang, Qingxin Meng, Tao Wang","doi":"10.3389/fbioe.2025.1615481","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1615481","url":null,"abstract":"<p><strong>Indroduction: </strong>The integration of sonodynamic therapy (SDT) and carbon monoxide (CO) presents a promising synergistic strategy in cancer therapy owing to the unique advantage of CO in SDT sensitization. However, the development of SDT-compatible CO-delivery nanosystems remain a substantial challenge.</p><p><strong>Methods: </strong>Here, we developed an ultrastable and controllable CO nanoreservoir system through the integration of chlorine e6 (Ce6)-loaded, cancer cell membrane coating and iron carbonyl (Fe₃CO₁₂)-bridged mesoporous silica bodies (Fe₃CO₁₂-MSNs), which was specifically engineered to simultaneously achieve SDT and ultrasound (US)-responsive sustained CO release. Owing to the stabilization of Fe₃CO₁₂ within the silica framework, Fe₃CO₁₂-MSNs not only decreased unwanted CO leakage during transport but also enabled US-responsive matrix degradation accompanied by sustained CO release at tumor sites, which prolongs the therapeutic window of CO and maximizes the synergy of SDT and CO therapy.</p><p><strong>Results and discussion: </strong>This nanoplatform-mediated combination therapies showed highly efficient antitumor effects and triggered a robust tumor-specific immune responses. When in combination with immune checkpoint blockers, the nanoplatform notably eradicate the breast cancer with low systematic toxicity. Overall, our work provides a promising nanoplatform with US-responsive and sustainable CO release for highly efficient and safe SDT/CO combined therapeis.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1615481"},"PeriodicalIF":4.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Hybrid films loaded with 5-fluorouracil and Reglan for synergistic treatment of colon cancer via asynchronous dual-drug delivery.","authors":"Hairong Mao, Jianfeng Zhou, Liang Yan, Shuping Zhang, Deng-Guang Yu","doi":"10.3389/fbioe.2025.1597014","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1597014","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fbioe.2024.1398730.].</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1597014"},"PeriodicalIF":4.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically modified microorganisms for agricultural use: an opportunity for the advancement of risk assessment criteria in Argentina.","authors":"Clara Rubinstein, Gabriela Levitus, Carmen Vicien, Natalia Andrea Modena, Sandra Ruzal, Facundo Vesprini, Dalia Marcela Lewi, Cecilia Caminoa, Maria Fabiana Malacarne, Nerina Francescutti, Juan Ignacio Amaturo","doi":"10.3389/fbioe.2025.1612226","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1612226","url":null,"abstract":"<p><p>The development and use of biologicals in agriculture is of growing interest globally. The potential of these tools to increase and protect yield complementing other tools has stimulated the interest of developers. Agricultural countries like Brazil and Argentina in Latin America have extensive experience with the use of biologicals for biocontrol and as seed inoculants. The last decade has seen the number of bio-based startups grow in the region, many of those dedicated to the development of microbial based bio-inputs. The potential for improving the efficacy and functionality of these products by means of gene technologies is very promising; however, the regulatory oversight of these innovations needs adaptation to become fit for purpose. The Biotechnology Working Group at ICCAS identified the need for a science-based discussion on this matter and considered alternatives to the current paradigm, developed over 30 years ago for transgenic plants.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1612226"},"PeriodicalIF":4.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of custom-designed oral stents in radiotherapy for head and neck malignancies.","authors":"Shihang Li, Guobao Pang, Rong Li, Qinying Shi, Yannan Xu, Ying Lu, Jianbo Song","doi":"10.3389/fbioe.2025.1544105","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1544105","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the 3D-printed oral stents (3D-OS) and personalised hand-made stents in dentistry (DentStent) for head and neck malignancies.</p><p><strong>Methods: </strong>Twelve patients with head and neck malignant tumours in the Cancer Centre of Shanxi Bethune Hospital from 1 August 2023 to 31 September 2024 were admitted to the study. After obtaining informed consent from the patients, 3D-OS and DentStent were designed for each patient, respectively, and the patients wore them to produce two groups of radiotherapy plans. The aim was to compare the homogeneity index (HI) and conformity index (CI) of the target areas, as well as the dose differences to the organs at risk (OAR) between the two plans. Additionally, the satisfaction of the patients with the stents after use was assessed.</p><p><strong>Results: </strong>The difference in HI, CI and OAR dose to the target area between the two groups was slight, but 3D-OS took less time (∼4 h) to fabricate and resulted in higher resolution and patient satisfaction.</p><p><strong>Conclusion: </strong>The proposed 3D-OS could obtain good HI and CI in radiotherapy for head and neck malignancies, and is a new method for rapid and satisfactory personalised oral stent.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1544105"},"PeriodicalIF":4.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal intelligent biosensors framework for fall disease detection and healthcare monitoring.","authors":"Iqra Aijaz Abro, Shuaa S Alharbi, Naif S Alshammari, Asaad Algarni, Nouf Abdullah Almujally, Ahmad Jalal, Hui Liu","doi":"10.3389/fbioe.2025.1544968","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1544968","url":null,"abstract":"<p><strong>Introduction: </strong>In the field of human action recognition, the fusion of multi-modal data from RGB and inertial modalities provides a valid technique for identifying activities of daily life and falls.</p><p><strong>Methods: </strong>Our approach uses two reference datasets: UR-Fall Detection and UMA_Fall Detection for ADL and Fall Events. First, data preprocessing is conducted for each sort of sensor individually, then the signals are windowed and segmented properly. Key features are then extracted, where from RGB data we get 2.5D point clouds, kinetic energy, angles, curve points, ridge features, and inertial signals, giving GCC, GMM, LPCC, and SSCE coefficients. The second method employed is Adam to improve the discriminant of the chosen features. For classification, we employed a Deep Neural Network (DNN) for ADL and fall detection over the UR-Fall dataset and the UMA_Fall dataset.</p><p><strong>Results: </strong>The classification accuracy achieved on the UMA_Fall dataset is 97% for ADL activities and 96% for fall activities, while for the UR-Fall dataset, it is 94% for ADL activities and 92% for fall activities. This diversified classifier setting compensates for the variety of data and optimizes the system for differentiating between ADL and fall events.</p><p><strong>Discussion: </strong>The above system provides outstanding results in recognizing these activities on both datasets and illustrates that the multimodal data fusion can boost the human activity identification system for health and safety purposes.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1544968"},"PeriodicalIF":4.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144527156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity.","authors":"Aleksandra Bandzerewicz, Anna Herman, Ewa Dutkowska, Klara Niebuda, Paweł Ruśkowski, Agnieszka Gadomska-Gajadhur","doi":"10.3389/fbioe.2025.1579630","DOIUrl":"10.3389/fbioe.2025.1579630","url":null,"abstract":"<p><p>Biomaterials with antimicrobial properties are a key research area due to the increasing threat of infections and the growing resistance of microorganisms to existing antibiotics. The aim of the study was to produce thermally cross-linked polymer films based on poly(1,5-pentanediol azelate) and poly(1,4-butanediol citrate) with antimicrobial activity for medical applications. Well-formed, cross-linked, flexible materials differing in appearance depending on the conditions of the cross-linking process were obtained. In general, a lower cross-linking temperature was found to promote less brittle and more flexible films with greater structure uniformity. The polymer films had hydrophilic surfaces (water contact angle 40°-60°). All polymer films maintained integrity after immersion in PBS buffer. Most likely, the lower hydrophilicity of the polyazelate phase limited their degradation. A modified time-kill procedure (ASTM E2315-23) was performed to test the antimicrobial properties of the films against <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, and <i>Candida albicans</i>. The antimicrobial activity of polycitrate-based films against <i>P</i>. <i>aeruginosa</i> has been reported with >90% reduction of the pathogen after 6 h of contact and 100% biocidal effect after 24 h. The antimicrobial activity of the film is pH-based. The biocidal effect of polycitrate film against <i>P. aeruginosa</i> is the most important and promising result, especially given the resistance of the pathogen to commonly used antibiotics.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1579630"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recombinant humanized collagen combined with nicotinamide increases the expression level of rat basement membrane proteins and promotes hair growth.","authors":"Youshiqi Zhou, Boyu Chen, Shijia Ye, Haoyu Sun, Shuyue Wang, Xiaozhen Diao, Wenhui Wu","doi":"10.3389/fbioe.2025.1546779","DOIUrl":"10.3389/fbioe.2025.1546779","url":null,"abstract":"<p><strong>Background: </strong>Hair follicle stem cells (HFSCs) play crucial roles in hair growth and are expected to be potential targets in regenerative medicine and tissue engineering.</p><p><strong>Method: </strong>This study aims to investigate the positive effect on hair growth by the recombinant human collagen complex (RHC complex), composed of rhCOL III, rhCOL XVII, and rhCOL XXI, along with nicotinamide, both <i>in vitro</i> and <i>in vivo</i>, by HFSCs and rat models. The survival rate, function, and differentiation of HFSCs were investigated.</p><p><strong>Results: </strong>The CCK-8 experiment showed that the RHC complex was non-toxic to HFSCs, and the cell survival rate exceeded 80% after 8 and 16 h of treatment. The ELISA method showed that the RHC complex significantly increased the intracellular vascular endothelial growth factor (VEGF) levels. In addition, the increase in the content of trichohyalin (a key structural protein of hair) indicates that the structure and function of hair follicles may be enhanced. The expression levels of β-integrin and p63 were significantly upregulated, which are crucial for cell adhesion, migration, and maintenance of HFSCs homeostasis. In the rat model, significant hair growth was observed after a 7-day treatment period. The period of vigorous hair growth in rats was selected for immunofluorescence, enzyme-linked immunosorbent assay (ELISA) and hematoxylin-eosin (HE) staining analysis. The results showed that the RHC complex could promote the expression of Integrin, Laminin and Perlecan, which were conducive to maintaining the stability of the microenvironment of HFSCs. Additionally it facilitated the migration and differentiation of HFSCs, as evidenced by an increased number of hair follicles in HE-stained skin tissues. In conclusion, the RHC complex exhibited high HFSCs survival rates and enhanced the expression of HFSCs-associated factors and basement membrane proteins. These properties make the RHC complex a promising novel ingredient for promoting hair growth, preventing hair loss, and maintaining hair health.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1546779"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pluripotency genes of mammals: a network at work.","authors":"Ranieri Cancedda, Maddalena Mastrogiacomo","doi":"10.3389/fbioe.2025.1578499","DOIUrl":"10.3389/fbioe.2025.1578499","url":null,"abstract":"<p><p>Pluripotency, i.e., the ability to differentiate into cells of all three germ layers, is a transient state of early embryonic cells. In mammals, during progression from pre-implantation to post-implantation stage, pluripotent cells undergo different state transitions characterized by changes in gene expression and development potential. These developmental states include: (i) a naive pluripotency (pre-implantation embryonic stem cells, or ESCs), (ii) an intermediate condition (formative state), and (iii) a primed pluripotency (late post-implantation ESCs derived from epiblasts also named EpiSCs). The transitions are regulated by an interconnected network of pluripotency-related genes. Transcription of genes such as <i>Oct4, Sox2</i>, and <i>Nanog</i> is crucial for obtaining and maintaining pluripotency. These three factors form an autoregulatory loop by binding to each other's promoters to activate their transcription. Other factors play a significant ancillary role in the transcription factor network preserving cell pluripotency. In the review, we will also mention some of the more relevant cytokines, molecules, signaling pathways, and epigenetic modifications that induce and control pluripotency gene expression. The main goal of this review is to bridge the gap between the fields of genetics and stem cell biology and to set the ground for the application of this knowledge to the development of strategies and drugs to be used in a clinical environment.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1578499"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}