Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Design of novel graded bone scaffolds based on triply periodic minimal surfaces with multi-functional pores.","authors":"Rongwu Lai, Jian Jiang, Yi Huo, Hao Wang, Sergei Bosiakov, Yongtao Lyu, Lei Li","doi":"10.3389/fbioe.2025.1503582","DOIUrl":"10.3389/fbioe.2025.1503582","url":null,"abstract":"<p><strong>Background: </strong>Various mechanical and biological requirements on bone scaffolds were proposed due to the clinical demands of human bone implants, which remains a challenge when designing appropriate bone scaffolds.</p><p><strong>Methods: </strong>In this study, novel bone scaffolds were developed by introducing graded multi-functional pores onto Triply Periodic Minimal Surface (TPMS) structures through topology optimization of unit cell. The performance of these scaffolds was evaluated using finite element (FE) analysis and computational fluid dynamics (CFD) method.</p><p><strong>Results: </strong>The results from FE analysis indicated that the novel scaffold exhibited a lower elastic modulus, potentially mitigating the issue of stress shielding. Additionally, the results from CFD demonstrated that the mass transport capacity of the novel scaffold was significantly improved compared to conventional TPMS scaffolds.</p><p><strong>Conclusion: </strong>In summary, the novel TPMS scaffolds with graded multi-functional pores presented in this paper exhibited enhanced mechanical properties and mass transport capacity, making them ideal candidates for bone repair. A new design framework was provided for the development of high-performance bone scaffolds.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1503582"},"PeriodicalIF":4.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facial expression recognition through muscle synergies and estimation of facial keypoint displacements through a skin-musculoskeletal model using facial sEMG signals.","authors":"Lun Shu, Victor R Barradas, Zixuan Qin, Yasuharu Koike","doi":"10.3389/fbioe.2025.1490919","DOIUrl":"10.3389/fbioe.2025.1490919","url":null,"abstract":"<p><p>The development of facial expression recognition (FER) and facial expression generation (FEG) systems is essential to enhance human-robot interactions (HRI). The facial action coding system is widely used in FER and FEG tasks, as it offers a framework to relate the action of facial muscles and the resulting facial motions to the execution of facial expressions. However, most FER and FEG studies are based on measuring and analyzing facial motions, leaving the facial muscle component relatively unexplored. This study introduces a novel framework using surface electromyography (sEMG) signals from facial muscles to recognize facial expressions and estimate the displacement of facial keypoints during the execution of the expressions. For the facial expression recognition task, we studied the coordination patterns of seven muscles, expressed as three muscle synergies extracted through non-negative matrix factorization, during the execution of six basic facial expressions. Muscle synergies are groups of muscles that show coordinated patterns of activity, as measured by their sEMG signals, and are hypothesized to form the building blocks of human motor control. We then trained two classifiers for the facial expressions based on extracted features from the sEMG signals and the synergy activation coefficients of the extracted muscle synergies, respectively. The accuracy of both classifiers outperformed other systems that use sEMG to classify facial expressions, although the synergy-based classifier performed marginally worse than the sEMG-based one (classification accuracy: synergy-based 97.4%, sEMG-based 99.2%). However, the extracted muscle synergies revealed common coordination patterns between different facial expressions, allowing a low-dimensional quantitative visualization of the muscle control strategies involved in human facial expression generation. We also developed a skin-musculoskeletal model enhanced by linear regression (SMSM-LRM) to estimate the displacement of facial keypoints during the execution of a facial expression based on sEMG signals. Our proposed approach achieved a relatively high fidelity in estimating these displacements (NRMSE 0.067). We propose that the identified muscle synergies could be used in combination with the SMSM-LRM model to generate motor commands and trajectories for desired facial displacements, potentially enabling the generation of more natural facial expressions in social robotics and virtual reality.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1490919"},"PeriodicalIF":4.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Functional biomaterials for drug delivery.","authors":"Yi Chen, Zhengwei Cai","doi":"10.3389/fbioe.2025.1550444","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1550444","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1550444"},"PeriodicalIF":4.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861077/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in regenerative medicine-based approaches for skin regeneration and rejuvenation.","authors":"Nathalia Silva Dutra Alves, Gustavo Roncoli Reigado, Mayara Santos, Izabela Daniel Sardinha Caldeira, Henrique Dos Santos Hernandes, Bruna Leticia Freitas-Marchi, Elina Zhivov, Felipe Santiago Chambergo, Viviane Abreu Nunes","doi":"10.3389/fbioe.2025.1527854","DOIUrl":"10.3389/fbioe.2025.1527854","url":null,"abstract":"<p><p>Significant progress has been made in regenerative medicine for skin repair and rejuvenation. This review examines core technologies including stem cell therapy, bioengineered skin substitutes, platelet-rich plasma (PRP), exosome-based therapies, and gene editing techniques like CRISPR. These methods hold promise for treating a range of conditions, from chronic wounds and burns to age-related skin changes and genetic disorders. Challenges remain in optimizing these therapies for broader accessibility and ensuring long-term safety and efficacy.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1527854"},"PeriodicalIF":4.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Humanising nanotoxicology: replacement of animal-derived products in the application of integrated approaches to testing and assessment of nanomaterial inhalation hazard.","authors":"Roma Fraser, Keira Campbell, Pawel Pokorski, Eve MacKinnon, Katie McAllister, Karla B Neves, Fiona Murphy","doi":"10.3389/fbioe.2025.1526808","DOIUrl":"10.3389/fbioe.2025.1526808","url":null,"abstract":"<p><p>Over the past decade, the development of nanomaterials (NMs) has surged, highlighting their potential benefits across multiple industries. However, concerns regarding human and environmental exposure remain significant. Traditional <i>in vivo</i> models for safety assessments are increasingly viewed as unfeasible and unethical due to the diverse forms and biological effects of NMs. This has prompted the design of Novel Approach Methods (NAMs) to streamline risk assessment and predict human hazards without relying on animal testing. A critical aspect of advancing NAMs is the urgent need to replace animal-derived products in assay protocols. Incorporating human or synthetic alternatives can significantly reduce the ethical burden of animal use while enhancing the relevance of toxicity testing. This study evaluates the impact of removing animal-derived products from standard acellular and <i>in vitro</i> assays recommended in a published Integrated Approaches to Testing and Assessment (IATA) for inhaled NMs. We specifically assessed the effects of replacing fetal bovine serum with human platelet lysate in acellular reactivity tests and <i>in vitro</i> toxicity testing using a panel of well-characterized NMs. Significant differences in acellular NM reactivity and dramatic changes in A549 cell growth rates and responses to NMs were observed under different media conditions. Our findings demonstrate that variations in experimental setup can fundamentally impact NM hazard assessment, influencing the interpretation of results within specific assays and across tiered testing strategies. Further investigation is needed to support a shift toward more ethical toxicity testing that does not rely on animal-derived materials.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1526808"},"PeriodicalIF":4.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of different carbon-fiber plate shapes in shoes on lower limb biomechanics following running-induced fatigue.","authors":"Yufan Xu, Chengyuan Zhu, Yufei Fang, Zhenghui Lu, Yang Song, Chen Hu, Dong Sun, Yaodong Gu","doi":"10.3389/fbioe.2025.1539976","DOIUrl":"10.3389/fbioe.2025.1539976","url":null,"abstract":"<p><p>Different shapes of carbon-fiber plates (CFPs) are likely to affect lower limb biomechanics, particularly under conditions of running-induced fatigue, and potentially impact runners' performance and risk of injury. However, no studies have yet elucidated the precise effects of CFP shapes on the lower limb biomechanical characteristics subsequent to running-induced fatigue. The purpose of this study was to investigate the effects of different CFP shapes in running shoes on the lower limb biomechanics of runners following running-induced fatigue. 12 male runners (aged 21.8 ± 1.3 years, mass 59.1 ± 4.1 kg, height 168.9 ± 2.2 cm, weekly running distance 68.8 ± 5.5 km/week) were recruited for this study. Two-way repeated measures ANOVA was used to compare kinematic and kinetic data, while SPM (Statistical Parametric Mapping) was used to assess the activation levels of lower limb muscles. Compared to wearing flat CFP shoes (\"Flat\"), wearing curved CFP shoes (\"Curve\") resulted in a significant reduction in the hip (p = 0.034) and knee contact angle (p < 0.000), as well as a significant decrease in the hip flexion moment (p = 0.008). The activation level of the tibialis anterior (TA) was significantly higher when wearing \"Curve\" in pre-fatigue compared to \"Flat\", whereas the opposite was observed post-fatigue. The curved CFP altered the bending angle of the forefoot, thereby significantly reducing the joint angles and joint moments of the hip and knee.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1539976"},"PeriodicalIF":4.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative 3D printing technologies and advanced materials revolutionizing orthopedic surgery: current applications and future directions.","authors":"Bo Cong, Haiguang Zhang","doi":"10.3389/fbioe.2025.1542179","DOIUrl":"10.3389/fbioe.2025.1542179","url":null,"abstract":"<p><p>Three-dimensional (3D) printing has rapidly become a transformative force in orthopedic surgery, enabling the creation of highly customized and precise medical implants and surgical tools. This review aims to provide a more systematic and comprehensive perspective on emerging 3D printing technologies-ranging from extrusion-based methods and bioink printing to powder bed fusion-and the broadening array of materials, including bioactive agents and cell-laden inks. We highlight how these technologies and materials are employed to fabricate patient-specific implants, surgical guides, prosthetics, and advanced tissue engineering scaffolds, significantly enhancing surgical outcomes and patient recovery. Despite notable progress, the field faces challenges such as optimizing mechanical properties, ensuring structural integrity, addressing regulatory complexities across different regions, and considering environmental impacts and cost barriers, especially in low-resource settings. Looking ahead, innovations in smart materials and functionally graded materials (FGMs), along with advancements in bioprinting, hold promise for overcoming these obstacles and expanding the capabilities of 3D printing in orthopedics. This review underscores the pivotal role of interdisciplinary collaboration and ongoing research in harnessing the full potential of additive manufacturing, ultimately paving the way for more effective, personalized, and durable orthopedic solutions that improve patient quality of life.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1542179"},"PeriodicalIF":4.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese-pyrochloric acid photosensitizer nanocomplexes against osteosarcoma: achieving both high activatability and high effectiveness.","authors":"Xuran Zhang, Jian Wang, Qun Feng, Li Lei, Zhiyong Zhu","doi":"10.3389/fbioe.2024.1485549","DOIUrl":"10.3389/fbioe.2024.1485549","url":null,"abstract":"<p><strong>Introduction: </strong>The application of photodynamic therapy (PDT) is limited by unsatisfactory therapeutic efficacy and dose-dependent phototoxicity in clinical settings. Intravenous nano-drug delivery systems (NDDSs) hold promise for enhancing the delivery efficiency of photosensitive drugs, but often result in aggregation-caused quenching (ACQ) effects, preventing site-specific activation.</p><p><strong>Methods: </strong>We exploited manganese (Mn²⁺)-pyrochloric acid (PPa) nanocomplexes coordinated using the photosensitizer PPa and metal Mn ion for the treatment of osteosarcoma. The nanocomplexes were precisely co-assembled in water to stably co-deliver Mn²⁺ and PPa, enabling tumor-specific release and fluorescence recovery.</p><p><strong>Results: </strong>Following laser irradiation, the activated PPa significantly enhanced the killing effects on primary cancer cells. Additionally, Mn²⁺ ions activated the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, promoting maturation of dendritic cells (DCs) and augmenting CD8⁺-mediated antitumor immune responses.</p><p><strong>Discussion: </strong>This study advances the on-demand activation of photosensitive drugs and photodynamic immunotherapy toward clinical applicability by exploiting Mn²⁺-PPa nanocomplexes with high activatability and effectiveness for targeted PDT and immunotherapy.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1485549"},"PeriodicalIF":4.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluid-structure interaction analysis for abdominal aortic aneurysms: the role of multi-layered tissue architecture and intraluminal thrombus.","authors":"Xinhai Yue, Jiayi Huang, Ju Liu","doi":"10.3389/fbioe.2025.1519608","DOIUrl":"10.3389/fbioe.2025.1519608","url":null,"abstract":"<p><strong>Introduction: </strong>Abdominal aortic aneurysm (AAA) is a life-threatening disease marked by localized dilatations of the infrarenal aortic wall. While clinical guidelines often use the aneurysm diameter as an indicator for surgical intervention, this metric alone may not reliably predict rupture risks, underscoring the need for detailed biomechanical analyses to improve risk assessments.</p><p><strong>Methods: </strong>We investigate the effects of the multi-layered tissue architecture and the intraluminal thrombus (ILT) on the wall stress distribution of AAA. Using fluid-structure interaction, we analyze the biomechanical responses of fusiform and saccular AAAs under three conditions: without ILT, with ILT but no tissue degradation, and with both ILT and tissue degradation.</p><p><strong>Results: </strong>The findings show that the media is the primary load-bearing layer, and the multi-layered model yields a more accurate stress profile than the single-layered tissue model. The ILT substantially reduces overall stress levels in the covered tissue, although its impact on the location of peak stress varies across different scenarios. Media degradation increases the stress in the intima and adventitia, but the cushioning effect of ILT largely mitigates this impact.</p><p><strong>Discussion: </strong>The results underscore the importance of incorporating the multi-layered tissue architecture and ILT in patient-specific analyses of AAA. These factors may improve the predictive capabilities of biomechanical assessments for rupture risk.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1519608"},"PeriodicalIF":4.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143499815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological assessment of a novel restorative coronary artery bypass graft in a chronic ovine model.","authors":"Yu Sato, Matthew Kutnya, Biniyam Abebe, Mohammed S El Kurdi, Martijn Cox, Richard W Bianco, Bart Meuris, Yoshinobu Onuma, Patrick W Serruys, Renu Virmani","doi":"10.3389/fbioe.2025.1488794","DOIUrl":"10.3389/fbioe.2025.1488794","url":null,"abstract":"<p><strong>Background: </strong>Although prosthetic conduits for coronary artery bypass grafting (CABG) are increasingly needed because of the limited availability and patency of autologous conduits, no alternatives have succeeded.</p><p><strong>Methods: </strong>Sixteen sheep underwent CABG. Thirteen received a bioabsorbable polymer graft with an incorporated nitinol microskeleton (Xeltis coronary artery bypass graft [XABG]), and three received autologous saphenous vein grafts (SVG). Pathological evaluation was conducted at 12 months.</p><p><strong>Results: </strong>In the XABG group, two sheep died perioperatively; two were sacrificed at 3 months (1 occluded, 1 patent) and two at 6 months (both patent). Two more died from occlusion at 9-10 months, and five survived with patent grafts at 12 months. All SVGs remained patent for 12 months. Histology demonstrated near-complete luminal endothelialization in XABG, with increased polymer adsorption and matrix deposition. The cross-sectional area of the SVG lumen was significantly larger than XABGs (48.2 mm² vs 12.9 mm², p = 0.0018), consistent with a reduced angiographic flow velocity in SVG. The neointimal area was greater in SVGs than XABGs (19.6 vs. 6.7 mm², p = 0.0005), especially at the distal ends of SVGs due to thrombus formation.</p><p><strong>Conclusion: </strong>XABG demonstrated 1-year feasibility with consistent endothelialization and polymer absorption. While SVGs had better patency, they showed greater diametrical irregularity and subsequent neointimal proliferation.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1488794"},"PeriodicalIF":4.3,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}