Simona Martano, Mariafrancesca Cascione, Livia Giotta, Loris Rizzello, Riccardo Di Corato, Stefano Leporatti, Rosaria Rinaldi, Valeria De Matteis
{"title":"表没食子儿茶素没食子酸酯负载BSA纳米颗粒作为未成熟巨噬细胞的创新抗炎剂。","authors":"Simona Martano, Mariafrancesca Cascione, Livia Giotta, Loris Rizzello, Riccardo Di Corato, Stefano Leporatti, Rosaria Rinaldi, Valeria De Matteis","doi":"10.3389/fbioe.2025.1666492","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The development of innovative anti-inflammatory therapies is critical for addressing chronic inflammatory diseases and cancer. Epigallocatechin gallate (EGCG), a polyphenolic compound with strong antioxidant and anti-inflammatory properties, suffers from limited stability and bioavailability. Bovine Serum Albumin Nanoparticles (BSA-NPs), due to their biodegradability, non-toxicity, and high binding capacity, represent a powerful delivery system for bioactive compounds.</p><p><strong>Methods: </strong>EGCG-loaded BSA nanoparticles (EGCG@BSA-NPs) were synthesized via the desolvation method. The nanoparticles were characterized by Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), ζ-potential analysis, Fourier-transform infrared spectroscopy (FTIR), and UV-Vis spectroscopy. Encapsulation efficiency and antioxidant capacity were assessed by Trolox equivalent antioxidant capacity (TEAC) assays. The anti-inflammatory potential was evaluated in immature macrophages (THP-1 cells) by assessing NF-κB nuclear translocation and the stimulation of proinflammatory cytokines IL-8 and TNF-α.</p><p><strong>Results: </strong>Morphological and physicochemical analyses confirmed the successful formation of spherical EGCG@BSA-NPs with improved size uniformity and controlled surface charge. Antioxidant assays demonstrated enhanced radical scavenging activity compared with unloaded BSA-NPs and free EGCG. Cellular studies showed that EGCG@BSA-NPs reduced NF-κB nuclear translocation and decreased IL-8/TNF-α secretion, highlighting their anti-inflammatory efficacy.</p><p><strong>Discussion: </strong>These findings suggest that EGCG@BSA-NPs are an effective nanoplatform for the controlled delivery of polyphenolic compounds. By improving stability and enhancing bioactivity, they hold significant promise in modulating macrophage function and reducing inflammation, thereby supporting their potential use in chronic inflammatory disease and cancer therapy.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1666492"},"PeriodicalIF":4.8000,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12537887/pdf/","citationCount":"0","resultStr":"{\"title\":\"Epigallocatechin-gallate loaded BSA nanoparticles as innovative anti-inflammatory agents in immature macrophages.\",\"authors\":\"Simona Martano, Mariafrancesca Cascione, Livia Giotta, Loris Rizzello, Riccardo Di Corato, Stefano Leporatti, Rosaria Rinaldi, Valeria De Matteis\",\"doi\":\"10.3389/fbioe.2025.1666492\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The development of innovative anti-inflammatory therapies is critical for addressing chronic inflammatory diseases and cancer. Epigallocatechin gallate (EGCG), a polyphenolic compound with strong antioxidant and anti-inflammatory properties, suffers from limited stability and bioavailability. Bovine Serum Albumin Nanoparticles (BSA-NPs), due to their biodegradability, non-toxicity, and high binding capacity, represent a powerful delivery system for bioactive compounds.</p><p><strong>Methods: </strong>EGCG-loaded BSA nanoparticles (EGCG@BSA-NPs) were synthesized via the desolvation method. The nanoparticles were characterized by Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), ζ-potential analysis, Fourier-transform infrared spectroscopy (FTIR), and UV-Vis spectroscopy. Encapsulation efficiency and antioxidant capacity were assessed by Trolox equivalent antioxidant capacity (TEAC) assays. The anti-inflammatory potential was evaluated in immature macrophages (THP-1 cells) by assessing NF-κB nuclear translocation and the stimulation of proinflammatory cytokines IL-8 and TNF-α.</p><p><strong>Results: </strong>Morphological and physicochemical analyses confirmed the successful formation of spherical EGCG@BSA-NPs with improved size uniformity and controlled surface charge. Antioxidant assays demonstrated enhanced radical scavenging activity compared with unloaded BSA-NPs and free EGCG. Cellular studies showed that EGCG@BSA-NPs reduced NF-κB nuclear translocation and decreased IL-8/TNF-α secretion, highlighting their anti-inflammatory efficacy.</p><p><strong>Discussion: </strong>These findings suggest that EGCG@BSA-NPs are an effective nanoplatform for the controlled delivery of polyphenolic compounds. 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Epigallocatechin-gallate loaded BSA nanoparticles as innovative anti-inflammatory agents in immature macrophages.
Introduction: The development of innovative anti-inflammatory therapies is critical for addressing chronic inflammatory diseases and cancer. Epigallocatechin gallate (EGCG), a polyphenolic compound with strong antioxidant and anti-inflammatory properties, suffers from limited stability and bioavailability. Bovine Serum Albumin Nanoparticles (BSA-NPs), due to their biodegradability, non-toxicity, and high binding capacity, represent a powerful delivery system for bioactive compounds.
Methods: EGCG-loaded BSA nanoparticles (EGCG@BSA-NPs) were synthesized via the desolvation method. The nanoparticles were characterized by Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), ζ-potential analysis, Fourier-transform infrared spectroscopy (FTIR), and UV-Vis spectroscopy. Encapsulation efficiency and antioxidant capacity were assessed by Trolox equivalent antioxidant capacity (TEAC) assays. The anti-inflammatory potential was evaluated in immature macrophages (THP-1 cells) by assessing NF-κB nuclear translocation and the stimulation of proinflammatory cytokines IL-8 and TNF-α.
Results: Morphological and physicochemical analyses confirmed the successful formation of spherical EGCG@BSA-NPs with improved size uniformity and controlled surface charge. Antioxidant assays demonstrated enhanced radical scavenging activity compared with unloaded BSA-NPs and free EGCG. Cellular studies showed that EGCG@BSA-NPs reduced NF-κB nuclear translocation and decreased IL-8/TNF-α secretion, highlighting their anti-inflammatory efficacy.
Discussion: These findings suggest that EGCG@BSA-NPs are an effective nanoplatform for the controlled delivery of polyphenolic compounds. By improving stability and enhancing bioactivity, they hold significant promise in modulating macrophage function and reducing inflammation, thereby supporting their potential use in chronic inflammatory disease and cancer therapy.
期刊介绍：
The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs.
In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.
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