Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Cancer immunogenic cell death via pyroptosis with CXCR4-targeted nanotoxins in hepatocellular carcinoma.","authors":"Yingbin Huang, Yihu Li, Rui He, Shuyi Dong, Zheng Zhao, Xingyuan Jiao","doi":"10.3389/fbioe.2024.1433126","DOIUrl":"10.3389/fbioe.2024.1433126","url":null,"abstract":"<p><strong>Introduction: </strong>Cytotoxic agents have shown limited benefits in hepatocellular carcinoma (HCC), mediated in part by the lack of targeting. As cell-penetrating peptides (CPPs) are capable of delivering various biologically active molecules into cells, including protein, peptides, small chemo-drugs, and nucleic acid with or without targeting, we developed T22-PE24, a CXCR4-targeted self-assembling cytotoxic nanotoxin, to effectively induce HCC pyroptosis.</p><p><strong>Methods: </strong>T22 incorporating <i>enhanced green fluorescent protein (</i>EGFP) or PE24 was purified from DE3 bacterial cells and characterized using transmission electron microscopy, the Zetasizer Nano^®, and SEC-HPLC. The internalization effect of T22-EGFP was detected by flow cytometry system (FCS) in CXCR4⁺/LM3(^CXCR4-) HCC cells. The CCK8, lactate dehydrogenase (LDH) release, Western blot, and nude mice HCC models were used to estimate the cell viability of T22-PE24. The complete-immunity HCC tumor-bearing mice model was used to assess the immune response of T22-PE24.</p><p><strong>Results: </strong>The round shape under transmission electron microscopy, 49.4 nm hydrodynamic diameter, and -33.33 mV zeta potential indicated that T22-PE24 self-assembled into nanoparticles. T22 incorporating EGFP selectively internalized in CXCR4⁺ HCC cells and showed no accumulation in CXCR4-knockout HCC cells. The T22-PE24 nanotoxin induced HCC pyroptosis via the caspase-3/GSDME signaling pathway and suppressed tumor growth in the absence of histological alterations in normal organs. Using the complete-immunity HCC tumor-bearing mice model, we found that T22-PE24 nanotoxin effectively induces the global reprogramming of cell components of the immune tumor microenvironment, leading to enhanced antitumor effects compared to those observed in immunodeficient mice.</p><p><strong>Conclusion: </strong>Our findings demonstrate the activation of the innate immune response in HCC by inducing pyroptosis with T22-PE24 nanotoxin treatment and support an implementation of this strategy for HCC treatment.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1433126"},"PeriodicalIF":4.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11570815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key factors for increased tip-apex distance when treating intertrochanteric fractures with InterTAN nails.","authors":"Naifeng Zhu, Lianxia Wu, Xiaofeng Han, Zhonglai Qian","doi":"10.3389/fbioe.2024.1426307","DOIUrl":"10.3389/fbioe.2024.1426307","url":null,"abstract":"<p><strong>Background: </strong>The tip-apex distance is a key factor in predicting implant cut-out after intramedullary fixation for intertrochanteric fractures. This study aimed to evaluate the factors associated with an increased tip-apex distance when treating intertrochanteric fractures using an InterTAN nail.</p><p><strong>Methods and material: </strong>We retrospectively analyzed patients with intertrochanteric fractures who underwent InterTAN nail insertion between January 2017 and March 2022 at our hospital. Medical and radiological data were collected. Measurements of preoperative factors and postoperative factors were performed accordingly. Multivariate logistic regression analysis was performed to determine the statistically significant variables of the tip-apex distance.</p><p><strong>Results: </strong>This study included 102 patients with intertrochanteric fractures. The average tip-apex distance measured 22.4 ± 7.1 mm, ranging from 9.3 to 48.0 mm. The length of the femoral neck on the non-fractured side, lag screw placement in the sagittal plane (center-inferior, superior) and coronal plane (posterior), and the angle between the line of the proximal nail axis and the femoral long axis were identified to be statistically significant factors for the tip-apex distance.</p><p><strong>Conclusion: </strong>To obtain a shorter tip-apex distance, we recommend a medial trochanteric entry point to minimize the angle between the line of the proximal nail axis and the femoral long axis. Additionally, sufficiently deep central insertion of the lag screw was advised in both the sagittal and coronal planes.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1426307"},"PeriodicalIF":4.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly parallel bending tests for fungal hyphae enabled by two-photon polymerization of microfluidic mold.","authors":"Steffen Brinkmann, Marcel Schrader, Sven Meinen, Ingo Kampen, Arno Kwade, Andreas Dietzel","doi":"10.3389/fbioe.2024.1449167","DOIUrl":"10.3389/fbioe.2024.1449167","url":null,"abstract":"<p><p>Filamentous microorganisms exhibit a complex macro-morphology constituted of branched and cross-linked hyphae. Fully resolved mechanical models of such mycelial compounds rely heavily on accurate input data for mechanical properties of individual hyphae. Due to their irregular shape and high adaptability to environmental factors, the measurement of these intrinsic properties remains challenging. To overcome previous shortcomings of microfluidic bending tests, a novel system for the precise measurement of the individual bending stiffness of fungal hyphae is presented in this study. Utilizing two-photon polymerization, microfluidic molds were fabricated with a multi-material approach, enabling the creation of 3D cell traps for spore immobilization. Unlike previous works applying the methodology of microfluidic bending tests, the hyphae were deflected in the vertical center of the microfluidic channel, eliminating the adverse influence of nearby walls on measurements. This lead to a significant increase in measurement yield compared to the conventional design. The accuracy and reproducibility of bending tests was ensured through validation of the measurement flow using micro-particle image velocimetry. Our results revealed that the bending stiffness of hyphae of <i>Aspergillus niger</i> is approximately three to four times higher than that reported for <i>Candida albicans</i> hyphae. At the same time, the derived longitudinal Young's Modulus of the hyphal cell wall yields a comparable value for both organisms. The methodology established in this study provides a powerful tool for studying the effects of cultivation conditions on the intrinsic mechanical properties of single hyphae. Applying the results to resolved numerical models of mycelial compounds promises to shed light on their response to hydrodynamic stresses in biotechnological cultivation, which influences their expressed macro-morphology and in turn, product yields.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1449167"},"PeriodicalIF":4.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attachment promoting compounds significantly enhance cell proliferation and purity of bovine satellite cells grown on microcarriers in the absence of serum.","authors":"Vincent Bodiou, Anitha Ajith Kumar, Edoardo Massarelli, Tessa van Haaften, Mark J Post, Panagiota Moutsatsou","doi":"10.3389/fbioe.2024.1443914","DOIUrl":"10.3389/fbioe.2024.1443914","url":null,"abstract":"<p><strong>Introduction: </strong>To bring cultivated beef to the market, a scalable system that can support growth of bovine satellite cells (bSCs) in a serum-free and preferably also animal-free medium is of utmost importance. The use of microcarriers (MCs) is, at the moment, one of the most promising technologies for scaling up. MCs offer a large surface to volume ratio, they can be used in scalable stirred tank bioreactors, where the culture conditions can be tightly controlled to meet the cells' requirements (temperature, pH, dissolved oxygen). The inherent capacity of the cells to migrate from one MC to another, also known as bead-to-bead transfer, facilitates a scale-up strategy involving MCs. Previous studies have shown growth of bSCs on three commercially available MCs in serum containing media. Unfortunately there is currently no information available regarding their growth on MCs in serum-free conditions.</p><p><strong>Methods: </strong>In this study, we aimed to find suitable serum-free media, MCs and attachment promoting compounds (APCs) supporting the growth of bSCs. Initially, six commercial MCs and three serum-free media were evaluated. The effects of three APCs were compared (vitronectin, laminin and fibronectin). Subsequently, the effects of different concentrations and modes of addition of the best performing APC were investigated.</p><p><strong>Results and discussion: </strong>Our results showed that Cytodex 1, Synthemax II and CellBIND supported bSCs' growth in all serum-free media. Overall, better growth was observed with Cytodex 1 in serum-free proliferation media. We showed that the use of laminin or vitronectin with Cytodex 1 can significantly improve cell growth and purity. Laminin also allowed attachment and growth of bSCs on Plastic MCs which had been previously unsuccessful without APCs. Finally, we optimized the use of vitronectin from a sustainability and process perspective, and showed that it can be used solely as a coating for Cytodex 1 (16-100 ng/cm²) MCs, instead of as a medium supplement, enhancing cell attachment and proliferation.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1443914"},"PeriodicalIF":4.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degree of twist in the Achilles tendon interacts with its length and thickness in affecting local strain magnitude: a finite element analysis.","authors":"Shota Enomoto, Shunya Furuuchi, Tatsuki Ishibashi, Shu Yamada, Toshiaki Oda","doi":"10.3389/fbioe.2024.1445364","DOIUrl":"10.3389/fbioe.2024.1445364","url":null,"abstract":"<p><strong>Introduction: </strong>The relationship between the twisting of the three subtendons of the Achilles tendon (AT) and local strain has received attention in recent years. The present study aimed to elucidate how the degree of twist in the AT affects strain using finite element (FE) analysis, while also considering other geometries (e.g., length, thickness, and width) and their combinations.</p><p><strong>Methods: </strong>A total of 59 FE models with different degrees of twist and geometries were created. A lengthening force (<i>z</i>-axis) of 1,000 N was applied to each subtendon (total: 3,000 N). The average value of the first principal Lagrange strain was calculated for the middle third of the total length of the model.</p><p><strong>Results: </strong>Statistical (stepwise) analysis revealed the effects of the degree of twist, other geometries, and their combinations on AT strain. The main findings were as follows: (1) a greater degree of twist resulted in higher average strains (<i>t</i> = 9.28, <i>p</i> < 0.0001) and (2) the effect of the degree of twist on the strain depended on dimensions of thickness of the most distal part of the AT (<i>t</i> = -4.49, <i>p</i> < 0.0001) and the length of the AT (<i>t</i> = -3.82, <i>p</i> = 0.0005). Specifically, when the thickness of the most distal part and length were large, the degree of twist had a small effect on the first principal Lagrange strain; however, when the thickness of the most distal part and length were small, a greater degree of twist results in higher first principal Lagrange strain.</p><p><strong>Conclusion: </strong>These results indicate that the relationship between the degree of twist and local strain is complex and may not be accurately assessed by FE simulation using a single geometry.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1445364"},"PeriodicalIF":4.3,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of neuropeptides in cutaneous wound healing: a focus on mechanisms and neuropeptide-derived treatments.","authors":"Liwei Xing, Bing Chen, Yuliang Qin, Xinyao Li, Sitong Zhou, Kai Yuan, Rong Zhao, Dongdong Qin","doi":"10.3389/fbioe.2024.1494865","DOIUrl":"10.3389/fbioe.2024.1494865","url":null,"abstract":"<p><p>An extensive network of cutaneous nerves, neuropeptides, and specific receptors richly innervates the skin and influences a variety of physiological and pathological processes. The sensory and autonomic nerve fibers secrete a variety of neuropeptides that are essential to the different phases of wound healing. In addition to initiating a neurogenic inflammatory response in the early stages of healing, neuropeptides also control wound healing by influencing immune cells, repair cells, and the growth factor network. However, the precise mechanism by which they accomplish these roles in the context of cutaneous wound healing is still unknown. Investigating the mechanisms of action of neuropeptides in wound healing and potential therapeutic applications is therefore urgently necessary. The present review discusses the process of wound healing, types of neuropeptides, potential mechanisms underlying the role of neuropeptides in cutaneous wound healing, as well as some neuropeptide-derived treatment strategies, such as hydrogels, new dressings, electro stimulation, and skin-derived precursors. Future in-depth mechanistic studies of neuropeptides in cutaneous wound healing may provide opportunities to develop therapeutic technologies that harness the roles of neuropeptides in the wound healing process.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1494865"},"PeriodicalIF":4.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical analysis of different techniques for residual bone defect from tibial plateau bone cyst in total knee arthroplasty.","authors":"Dehua Liu, Zhuang Miao, Wenfei Zhang, Chuanwen Liu, Longzhuo Du, Yuanlong Zhu, Yange Luo, Weibo Zheng, Jianli Zhou, Peilai Liu, Xuezhou Li, Ming Li","doi":"10.3389/fbioe.2024.1498882","DOIUrl":"10.3389/fbioe.2024.1498882","url":null,"abstract":"<p><strong>Background: </strong>In patients with tibial plateau bone cysts undergoing total knee arthroplasty (TKA), bone defects commonly occur following tibial plateau resection. Current strategies for addressing these defects include bone grafting, bone cement filling, and the cement-screw technique. However, there remains no consensus on the optimal approach to achieve the best surgical outcomes. This study aims to evaluate the most effective repair method for residual bone defects following tibial plateau bone cyst repair during TKA from a biomechanical perspective.</p><p><strong>Methods: </strong>The treatment options for tibial plateau bone defects were classified into four categories: no treatment, cancellous bone filling, bone cement filling, and the cement-screw technique. Finite-element analysis (FEA) was employed to evaluate stress distribution and displacement across the models for each treatment group. In addition, static compression mechanical tests were used to assess the displacement of the models within each group.</p><p><strong>Results: </strong>FEA results indicate that when employing the cement-screw technique to repair tibial plateau bone defects, the maximum stress on the prosthesis and the cement below the prosthesis is minimized, while the maximum stress on the cancellous bone is maximized. And the displacement of each component is minimized. Biomechanical tests results further demonstrate that the displacement of the model is minimized when utilizing the cement-screw technique for tibial plateau bone defects.</p><p><strong>Conclusion: </strong>Using cement-screw technique in treating residual tibial bone defects due to bone cysts in TKA offers optimal biomechanical advantages.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1498882"},"PeriodicalIF":4.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boron in wound healing: a comprehensive investigation of its diverse mechanisms.","authors":"Nasrin Sedighi-Pirsaraei, Amirhossein Tamimi, Faraz Sadeghi Khamaneh, Sana Dadras-Jeddi, Naz Javaheri","doi":"10.3389/fbioe.2024.1475584","DOIUrl":"10.3389/fbioe.2024.1475584","url":null,"abstract":"<p><p>Chronic wounds present a significant clinical challenge due to their prolonged healing time and susceptibility to infection. Boron, a trace element with diverse biological functions, has emerged as a promising therapeutic agent in wound healing. This review article comprehensively investigates the mechanisms underlying the beneficial effects of boron compounds in wound healing. Boron exerts its healing properties through multiple pathways, including anti-inflammatory, antimicrobial, antioxidant, and pro-proliferative effects. Inflammation is a crucial component of the wound-healing process, and boron has been shown to modulate inflammatory responses by inhibiting pro-inflammatory cytokines and promoting the resolution of inflammation. Furthermore, boron exhibits antimicrobial activity against a wide range of pathogens commonly associated with chronic wounds, thereby reducing the risk of infection and promoting wound closure. The antioxidant properties of boron help protect cells from oxidative stress, a common feature of chronic wounds that can impair healing. Additionally, boron stimulates cell proliferation and migration, as well as essential tissue regeneration and wound closure processes. Overall, this review highlights the potential of boron as a novel therapeutic approach for treating chronic wounds, offering insights into its diverse mechanisms of action and clinical implications.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1475584"},"PeriodicalIF":4.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility of 3D-printed vs. thermoformed and heat-cured intraoral appliances.","authors":"Joanna Weżgowiec, Andrzej Małysa, Wojciech Szlasa, Julita Kulbacka, Agnieszka Chwiłkowska, Marek Ziętek, Mieszko Więckiewicz","doi":"10.3389/fbioe.2024.1453888","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1453888","url":null,"abstract":"<p><strong>Objectives: </strong>The development of additive manufacturing has the potential to revolutionize the fabrication of medical devices. This technology, also known as 3D printing, offers precise, cost-effective, and personalized approaches, which could be particularly beneficial in the production of intraoral appliances. Despite its promise, research on the biocompatibility of 3D-printed intraoral devices is still limited. Our study aims to address this gap.</p><p><strong>Methods: </strong>We examined the cytotoxicity of materials processed via three techniques commonly used for the fabrication of different intraoral appliances: 3D printing (Dental LT Clear), thermoforming (Duran adjusted with Durasplint LC), and conventional heat-curing (Villacryl H Plus). We also investigated the impact of chemical or UVC disinfection on the biocompatibility of these materials. We assessed the biological effects induced in human gingival fibroblasts (HGFs) through both direct contact tests (MTT and LDH assays) and extract tests (PrestoBlue, DCF, and cell death type assays). Additionally, we observed changes in cellular morphology and migration rate under an inverted light microscope. The surface roughness of materials was evaluated using contact profilometry. Statistical analysis was conducted using two-way analysis of variance.</p><p><strong>Results: </strong>Our findings suggest that all three fabrication techniques induced a slight cytotoxic effect in HGFs, as evidenced by both direct contact and extract tests. However, these materials could be considered nontoxic according to the ISO 10993-5:2009 norm, as the decrease in metabolic activity observed was always less than 30% compared to the untreated control.</p><p><strong>Conclusion: </strong>This novel study confirms that 3D printing may be a safe alternative to conventional methods for fabricating intraoral appliances. However, further tests assessing the long-term intraoral usage are still needed.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1453888"},"PeriodicalIF":4.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthy but not osteoarthritic human meniscus-derived matrix scaffolds promote meniscus repair.","authors":"Saman Firoozi, Jon C Ley, Dawn A D Chasse, David E Attarian, Samuel S Wellman, Annunziato Amendola, Amy L McNulty","doi":"10.3389/fbioe.2024.1495015","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1495015","url":null,"abstract":"<p><p>Meniscus tissue is commonly injured due to sports-related injuries and age-related degeneration and approximately 50% of individuals with a meniscus tear will develop post-traumatic osteoarthritis (PTOA). Given that the meniscus has limited healing potential, new therapeutic strategies are required to enhance meniscus repair. Porcine meniscus-derived matrix (MDM) scaffolds improve meniscus integrative repair, but sources of human meniscus tissue have not been investigated. Therefore, the objectives of this study were to generate healthy and osteoarthritic (OA) MDM scaffolds and to compare meniscus cellular responses and integrative repair. Meniscus cells showed high viability on both healthy and OA scaffolds. While DNA content was higher in cell-seeded OA scaffolds than cell-seeded healthy scaffolds, CCK-8, and both sGAG and collagen content were similar between scaffold types. After 28 days in an <i>ex vivo</i> meniscus defect model, healthy and OA scaffolds had similar DNA, sGAG, and collagen content. However, the shear strength of repair was reduced in defects containing OA scaffolds compared to healthy scaffolds. In conclusion, healthy human allograft tissue is a useful source for generating MDM scaffolds that can support cellular growth, ECM production, and <i>ex vivo</i> integrative repair of the meniscus, highlighting the potential suitability for tissue engineering approaches to improve meniscus repair.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1495015"},"PeriodicalIF":4.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}