Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Piezoelectric poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) microspheres for collagen regeneration and skin rejuvenation.","authors":"Zeyu Fu, Yingwei Qu, Yinghao Wu, Yuan Xu, Hengdi Zhang, Yaozong Tang, Ziying Jin, Jia Zhao, Chang Tan","doi":"10.3389/fbioe.2025.1554825","DOIUrl":"10.3389/fbioe.2025.1554825","url":null,"abstract":"<p><strong>Introduction: </strong>Skin aging is an inevitable physiological process driven by factors like cellular senescence, ultraviolet radiation (UV) radiation, and environmental pollutants. A key feature is the accelerated collagen degradation in the dermal extracellular matrix, leading to visible signs such as sagging, wrinkles, and hyperpigmentation. Traditional fillers, such as hyaluronic acid and collagen-based fillers, offer only temporary volume enhancement without stimulating collagen regeneration. Studies have shown that electrical signals generated by piezoelectric materials can promote tissue regeneration.</p><p><strong>Methods: </strong>This study explored the potential of piezoelectric PHBHHx microspheres as an innovative skin filler for enhancing collagen regeneration and improving maxillofacial aesthetics, with the aid of low-intensity pulsed ultrasound (LIPUS) stimulation. A comprehensive characterizations of the piezoelectric PHBHHx microspheres were conducted, and their potential to stimulate collagen regeneration was assessed using a subcutaneous injection model in New Zealand white rabbits.</p><p><strong>Results: </strong>The results indicated that PHBHHx microspheres exhibited stable degradation properties, great piezoelectric properties and excellent biocompatibility. Moreover, when stimulated by LIPUS, the collagen-regenerating effect of PHBHHx microspheres was further enhanced, histological analysis revealed a denser and more organized collagen structures in LIPUS-stimulated PHBHHx group.</p><p><strong>Discussion: </strong>These findings highlight the potential of PHBHHx microspheres as an advanced biomaterial for applications in aesthetic medicine, particularly in promoting collagen regeneration and enhancing skin rejuvenation.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1554825"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustained release of ubiquitin-like protein ISG-15 enhances tendon-to-bone healing following anterior cruciate ligament reconstruction in a mouse model.","authors":"Jun-Cheng Yao, Jie-Xin Zhang, Xuan Wang, Yu-Hao Wu, Hao-Lin Ke, Jia-Rong Liang, Yan Shao, Jin-Tao Li, Yuan Liu, Dao-Zhang Cai, Jian-Ying Pan","doi":"10.3389/fbioe.2025.1550584","DOIUrl":"10.3389/fbioe.2025.1550584","url":null,"abstract":"<p><p>The process of tendon-to-bone healing is regulated by several proteins and cytokines that play critical roles in shaping biomechanical properties and functional recovery. Among these, the ubiquitin-like protein ISG-15 has been reported to have a beneficial effect on tissue repair. However, its specific function in tendon-to-bone interface regeneration has not been well characterized. This study investigated the function of ISG15 <i>in vitro</i> and addressed its <i>in vivo</i> effects on tendon and bone healing. In this study, wild-type C57/BL6 mice underwent anterior cruciate ligament (ACL) reconstruction surgery, with a sustained-release hydrogel containing ISG15 protein injected into the bone tunnels in the treatment group. To assess its therapeutic potential, bone-tendon interface growth was evaluated through histological staining, while micro-computed tomography (Micro-CT) was employed to quantify newly formed bone and bone density within the bone tunnels. Additionally, biomechanical testing was performed to measure the mechanical strength of the grafted tendons, and immunohistochemistry was conducted to detect the expression of Runx2 and osteocalcin (OCN) at the bone-tendon interface. <i>In vitro</i> results showed that an appropriate concentration of ISG-15 has the ability to promote osteogenic differentiation of bone marrow mesenchymal stem cells. Also, In the <i>in vivo</i> experiments, the local application of ISG15 protein significantly reduced inflammatory tissue growth during the early stages of healing and minimized bone resorption in the later stages. Furthermore, Micro-CT analysis showed an increased volume of newly formed bone in the treatment group, while biomechanical testing demonstrated enhanced mechanical strength of the grafted tendons. In summary, this study suggests that the localized sustained release of ISG15 protein during ACL reconstruction facilitates tendon-to-bone interface repair by promoting bone ingrowth, ultimately leading to improved biomechanical properties and functional recovery.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1550584"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using 3D and 4D digital human modeling in extended reality-based rehabilitation: a systematic review.","authors":"Mengdi Lu, Wim Saeys, Maria Maryam, Inva Gjeleshi, Hoda Nazarahari, Steven Truijen, Sofia Scataglini","doi":"10.3389/fbioe.2025.1496168","DOIUrl":"10.3389/fbioe.2025.1496168","url":null,"abstract":"<p><strong>Introduction: </strong>Extended reality (XR) is increasingly used in rehabilitation, showing potential to enhance clinical outcomes. Recently, integrating digital human modeling (DHM) with XR has gained attention. This systematic review aimed to evaluate the effectiveness of combining 3D and 4D DHM with XR in rehabilitation.</p><p><strong>Methods: </strong>A systematic literature search was conducted according to PRISMA 2020 guidelines on the 28^th of May 2024 in five databases (PubMed, IEEE Database, Cochrane Library, Web of Science, and Science Direct). All types of experimental studies investigating the effectiveness of XR using 3D and 4D DHM in rehabilitation were included. Consensus-based Standards for the selection of health Measurement Instruments (COSMIN) and Evidence-Based Guideline Development (EBRO) were used to evaluate the methodological quality of the studies included.</p><p><strong>Results: </strong>Of the 1048 articles found, 16 were included in this review. These studies focused on 3D DHM in XR-based rehabilitation across various conditions and demonstrated superior effectiveness, especially in individuals with neglect, anorexia nervosa, bulimia nervosa, and type 2 diabetes in comparison with conventional therapy. DHM, captured via 3D cameras and combined with motion analysis or Wii remotes, was integrated into XR systems like VR games and avatar therapy. The studies reveal positive impacts on functional (e.g., upper limb function, gait, balance, quality of life), physical (e.g., pain reduction, spasticity, joint range), psychological (e.g., depression, emotional regulation, body image), and general health outcomes (e.g., body composition, metabolic health).</p><p><strong>Conclusion: </strong>Despite variability in study parameters, limited evidence suggests that 3D DHM in XR-based rehabilitation may enhance physical and psychological recovery across various pathologies. This review highlights the potential of DHM and XR integration but underscores the need for further research with larger samples, longer follow-ups, and standardized measures to confirm these technologies' reliability and effectiveness in rehabilitation.</p><p><strong>Systematic review registration: </strong>https://www.crd.york.ac.uk/PROSPERO/view/CRD42024553551, identifier CRD42024553551.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1496168"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Africa and zero hunger agenda: genome editing policy landscape, challenges and opportunities.","authors":"Olalekan Akinbo, Bongani Nkhabindze, Josephine Amedu, Rufus Ebegba, Agnes Asagbra, Billy Omboki Ratemo, Susan Angira Dada, Anne Muia, Roy Mugiira, Lilian Chimphepo, Leeford Oko Wristberg, Mathurin W Rouamba, Koussao Some, Valter N A Nuaila, Alsácia Atanásio, Olufunke Bolatito Shittu, Charles Oluwaseun Adetunji, Loubna Hamidi, Elinasi Monga, Silas Obukosia, Muyiwa Seyi Adegbaju, Samuel Acheampong, Estherine Fotabong","doi":"10.3389/fbioe.2025.1526851","DOIUrl":"10.3389/fbioe.2025.1526851","url":null,"abstract":"<p><p>Africa has historically struggled to adopt innovative agricultural technologies, which has significantly hindered efforts to ensure food security and improve livelihoods over the past century. A major obstacle in this regard has been the persistent skepticism surrounding the potential benefits of agricultural biotechnology. The challenges contributing to this skepticism include a notable knowledge gap among stakeholders, widespread technophobia, or fear of technology, as well as inconsistencies with global agreements such as the Convention on Biological Diversity (CBB). Although these challenges are not exclusive to Africa, they disproportionately impact the continent, making the need for effective solutions even more urgent. This paper investigates the national government policy landscape in five African countries that are poised to create a regulatory environment conducive to deploying genome editing technology for improved agricultural productivity. This exploration aligns with the continental agricultural policy initiatives, notably the \"CAADP Malabo Declaration\" and the soon-to-be-signed \"CAADP Kampala Declaration.\" Aligning with the African Union's continental agenda on agricultural transformation, as outlined in the Malabo Declaration and other key documents, is crucial for adopting innovative agricultural technologies like genome editing. Such alignment becomes increasingly critical for realizing the objectives set forth in the post-Malabo Declaration, with the Kampala Declaration playing a vital role in its implementation. This cohesive approach will not only foster agricultural innovation but also expedite development across the continent, addressing the pressing needs of food security and livelihoods in Africa.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1526851"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA vaccine platforms: linking infectious disease prevention and cancer immunotherapy.","authors":"Dariush Haghmorad, Majid Eslami, Niloufar Orooji, Iryna Halabitska, Iryna Kamyshna, Oleksandr Kamyshnyi, Valentyn Oksenych","doi":"10.3389/fbioe.2025.1547025","DOIUrl":"10.3389/fbioe.2025.1547025","url":null,"abstract":"<p><p>The advent of mRNA vaccines, accelerated by the global response to the COVID-19 pandemic, marks a transformative shift in vaccine technology. In this article, we discuss the development, current applications, and prospects of mRNA vaccines for both the prevention and treatment of infectious diseases and oncology. By leveraging the capacity to encode antigens within host cells directly, mRNA vaccines provide a versatile and scalable platform suitable for addressing a broad spectrum of pathogens and tumor-specific antigens. We highlight recent advancements in mRNA vaccine design, innovative delivery mechanisms, and ongoing clinical trials, with particular emphasis on their efficacy in combating infectious diseases, such as COVID-19, Zika, and influenza, as well as their emerging potential in cancer immunotherapy. We also address critical challenges, including vaccine stability, optimization of immune responses, and the broader issue of global accessibility. Finally, we review potential strategies for advancing next-generation mRNA vaccines, with the aim of overcoming current limitations in vaccine technology and enhancing both preventive and therapeutic approaches for infectious and oncological diseases.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1547025"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different reduction patterns on stress distribution in patients with intertrochanteric fractures with intramedullary nail fixation: a finite element analysis.","authors":"Jiajing Zhu, Zhipeng Du, Changpeng Cao, Yang Gao, Xinxiao Chen, Haiyang Xing, Gang Wang","doi":"10.3389/fbioe.2025.1507774","DOIUrl":"10.3389/fbioe.2025.1507774","url":null,"abstract":"<p><strong>Objective: </strong>Positive medial cortical support is a reliable fracture reduction pattern, but existing research on its use is mainly qualitative. We conducted this finite element analysis study to quantitatively evaluate the usefulness of positive cortical support for intertrochanteric fracture reduction.</p><p><strong>Methods: </strong>Twenty-five models of intramedullary nail fixation for AO type 31-A1.2 intertrochanteric fractures subjected to different reduction patterns were established. The peak von Mises stress at the femoral fracture surface, proximal intersection of the intramedullary nail, and distal intersection of the intramedullary nail, as well as the maximum fracture displacement, were determined by finite element analysis under the three working conditions of standing, walking, and walking stairs.</p><p><strong>Results: </strong>As the head-neck fragment moved forward, the von Mises stress at the fracture surface, the proximal intersection point of the intramedullary nail, and the distal intersection point gradually decreased. This resulted in reduced fracture displacement, a significant decrease in trabecular bone volume, and a slight increase in the risk of screw cut-out. As the head-neck fragment moved medially, the fracture gained positive support from the medial cortex, leading to a gradual decrease in von Mises stress at the fracture surface and the proximal intersection point of the intramedullary nail, as well as reduced fracture displacement. However, the von Mises stress at the distal intersection point gradually increased.</p><p><strong>Conclusion: </strong>The reduction pattern involving positive medial, anteromedial, and anterior cortical support may be an effective alternative to anatomical reduction for the treatment of difficult-to-reduce intertrochanteric fractures.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1507774"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response surface-based media optimization for astaxanthin production in <i>Corynebacterium glutamicum</i>.","authors":"Florian Meyer, Ina Schmitt, Volker F Wendisch, Nadja A Henke","doi":"10.3389/fbioe.2025.1516522","DOIUrl":"10.3389/fbioe.2025.1516522","url":null,"abstract":"<p><strong>Introduction: </strong>Astaxanthin is a C40 carotenoid that is used in animal feeds or cosmetics. Due to its high antioxidant property it is used for, e.g., anti-aging formulations and due to its intense red color it is used, e.g., in animal feed. While about 95% of commercial astaxanthin is currently chemically synthesized from fossil sources, the interest in natural and sustainable astaxanthin is growing. <i>Corynebacterium glutamicum</i>, an attractive host used in large-scale processes, e.g., industrial amino acid production, has been engineered for astaxanthin production.</p><p><strong>Methods: </strong>Here, a design of experiment (DoE) approach was applied to optimize the standard minimal medium for astaxanthin production. The concentrations of carbon, nitrogen and phosphorus sources, magnesium, calcium, the iron chelator protocatechuic acid, the vitamin biotin, and the trace metals were varied and astaxanthin production was evaluated.</p><p><strong>Results and discussion: </strong>By increasing the concentration of iron and decreasing that of manganese especially, it was possible to increase astaxanthin titers from 7.9 mg L^-1-39.6 mg L^-1 in a micro cultivation system and from 62 mg L^-1-176 mg L^-1 in a fed-batch fermentation.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1516522"},"PeriodicalIF":4.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A supervised machine-learning analysis of doxorubicin-loaded electrospun nanofibers and their anticancer activity capabilities.","authors":"Mohammadreza Rostami, Maliheh Gharibshahian, Mehrnaz Mostafavi, Ali Sufali, Mahsa Golmohammadi, Mohammad Reza Barati, Reza Maleki, Nima Beheshtizadeh","doi":"10.3389/fbioe.2025.1493194","DOIUrl":"10.3389/fbioe.2025.1493194","url":null,"abstract":"<p><p>Thanks to the diverse advantages of electrospun nanofibers, multiple drugs have been loaded in these nanoplatforms to be delivered healthily and effectively. Doxorubicin is a drug used in chemotherapy, and its various delivery and efficacy parameters encounter challenges, leading to the seeking of novel delivery methods. Researchers have conducted numerous laboratory investigations on the encapsulation of doxorubicin within nanofiber materials. This method encompasses various parameters for the production of fibers and drug loading, categorized into device-related, material-related, and study design parameters. This study employed a supervised machine-learning analysis to extract the influencing parameters of the input from quantitative data for doxorubicin-loaded electrospun nanofibers. The study also determined the significance coefficient of each parameter that influences the output results and identified the optimum points and intervals for each parameter. Our Support Vector Machine (SVM) analysis findings showed that doxorubicin-loaded electrospun nanofibers could be optimized through employing a machine learning-based investigation on the polymer solution parameters (such as density, solvent, electrical conductivity, and concentration of polymer), electrospinning parameters (such as voltage, flow rate, and distance between the needle tip and collector), and our study parameters, i.e., drug release and anticancer activity, which affect the properties of the drug-loaded nanofibers, such as the average diameter of fiber, anticancer activity, drug release percentage, and encapsulation efficiency. Our findings indicated the importance of factors like distance, polymer density, and polymer concentration, respectively, in optimizing the fabrication of drug-loaded electrospun nanofibers. The smallest diameter, highest encapsulation efficiency, highest drug release percentage, and highest anticancer activity are obtained at a molecular weight between 80 and 474 kDa and a doxorubicin concentration of at least 3.182 wt% with the polymer density in the range of 1.2-1.52 g/cm³, polymer concentration of 6.618-9 wt%, and dielectric constant of solvent more than 30. Also, the optimal distance of 14-15 cm, the flow rate of 3.5-5 mL/h, and the voltage in the range of 20-25 kV result in the highest release rate, the highest encapsulation efficiency, and the lowest average diameter for fibers. Therefore, to achieve optimal conditions, these values should be considered. These findings open up new roads for future design and production of drug-loaded polymeric nanofibers with desirable properties and performances by machine learning methods.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1493194"},"PeriodicalIF":4.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental investigation of a powered lifting assistance device introducing direct touch between the caregiver and the care receiver.","authors":"Mari Kurata, Ming Jiang, Kotaro Hoshiba, Yusuke Sugahara, Takahiro Uehara, Masato Kawabata, Ken Harada, Yukio Takeda","doi":"10.3389/fbioe.2025.1556501","DOIUrl":"10.3389/fbioe.2025.1556501","url":null,"abstract":"<p><strong>Introduction: </strong>Transferring a patient from one place to another is one of the most strenuous works in nursing care. To address this issue, we proposed a concept for a lifting assistance device that uses two cables to perform operations such as translation, rotation, and stay. It facilitates direct touch between the caregiver and the care receiver, allowing intuitive adjustments of position and posture based on the caregiver's intention, detected through variations in cable tension.</p><p><strong>Methods: </strong>To investigate the effectiveness of this concept, lifting experiments using a fabricated prototype were conducted. Twelve subjects, including four physical therapists (PTs) and eight subjects having no transfer experience, acted as caregivers, and a dummy was used as the care receiver.</p><p><strong>Results: </strong>Results show that regardless of the transfer experience, the caregiver's intention detection and adjustment of the care receiver's position and posture were successfully achieved with an accuracy of over 70%.</p><p><strong>Discussion: </strong>Survey feedback collected after the lifting experiments confirmed that utilizing direct touch between the caregiver and the care receiver was highly valued by all subjects, with a 5-point Likert scale rating both PTs (average score: 4.8 points) and non-experienced subjects (average score: 4.3 points).</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1556501"},"PeriodicalIF":4.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Noncontact optical 3D strain measurements in cervical soft tissues biomechanics by digital image correlation under tensile test: an experimental approach.","authors":"Fangzheng Lin, Yaoqian Cai, Jing Li, Jiheng Zhan, Zibo Gao, Xiaolong Zeng, Minshan Feng, Yongjin Li, Dingkun Lin, Ji Qi","doi":"10.3389/fbioe.2025.1493476","DOIUrl":"10.3389/fbioe.2025.1493476","url":null,"abstract":"<p><strong>Background: </strong>Digital image correlation (DIC) is widely used to measure surface strain in loaded objects. When studying the deformation of the cervical spine, the complexity and non-planarity of the structure complicate the speckle pattern required for applying DIC. While this non-invasive technique has shown promise in biomechanical testing, its application to cervical spine analysis presents unique challenges, particularly in achieving dynamic full-scale multi-aspect strain measurements. The aim of this paper is to introduce a method for exploring the stress-strain relationship on cervical cadaveric specimen by optical non-contact measurement system.</p><p><strong>Method: </strong>Cervical cadaveric specimens were selected as subjects. Before testing, anatomical exposure, embedding, and spraying were performed sequentially. Specimen preparation was optimized through transverse process removal to enhance visualization of key anatomical structures. The surface strain under tensile testing was analyzed by the Aramis non-contact measurement system.</p><p><strong>Result: </strong>High-quality three-dimensional strain images were obtained with improved inspection points across all aspects, particularly in the lateral aspect (5397.25 ± 723.76 vs. 3268.25 ± 573.17, <i>P</i> < 0.05). Under 60N tensile loading, strain distribution revealed concentration in soft tissue regions while preserving clear visualization of vertebral bodies, intervertebral discs, and foramina. Quantitative analysis shown consistent deformation patterns across cervical segments (C4-C7), with no significant differences in segmental parameters (<i>P</i> > 0.05).</p><p><strong>Conclusion: </strong>The application of an optical non-contact measurement system in this study of cervical spine biomechanics has been proven effective. This method potentially mitigates some of the limitations associated with previous DIC techniques when applied to cervical cadaveric specimens. As a result, it enables more available measurements of multidimensional strain, which may enhance our understanding of the mechanics of the cervical spine.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1493476"},"PeriodicalIF":4.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}