Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Non-invasive bio-electromagnetic monitoring of cerebrovascular function: a novel conductivity reactivity index (CRx) for optimal cerebral perfusion pressure in acute brain injury models.","authors":"Jia Xu, Haochen Li, Feng Wang, Lian Yan, Gui Jin, Mingsheng Chen, Gen Li, Mingxin Qin","doi":"10.3389/fbioe.2025.1564510","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1564510","url":null,"abstract":"<p><strong>Introduction: </strong>The pressure reactivity index (PRx) is a key predictor of cerebrovascular function, widely used to guide and optimize therapeutic strategies in patients with acute brain injury. This study investigates a non-invasive bio-electromagnetic technique for monitoring and maintaining cerebrovascular function in a rabbit model of acute brain injury.</p><p><strong>Methods: </strong>A coaxial parallel double-coil sensor was designed to detect changes in intracranial electromagnetic properties, measured as magnetic induction phase shifts (MIPS), which reflect cerebral blood volume fluctuations. A cerebrovascular function monitoring platform was constructed with this sensor, a vector network analyzer, a LabVIEW software platform, and a physiological signal acquisition device to record the MIPS and arterial blood pressure (ABP). In the animal experiment, a novel cerebrovascular function index Conductivity Reactivity index (CRx), established with MIPS and ABP, was to assess optimal cerebral blood perfusion pressure (CPP) for maintaining the cerebrovascular function in four gradients of CPP in acute brain injury model.</p><p><strong>Results: </strong>The results found that the CRx (-0.072 ± 0.203) was a significant negative correlation with the PRx (0.223 ± 0.203) (r = -0.447, P = 0.003). Under the optimal CPP determined by the CPP-CRx curve, the mean CRx (0.104 ± 0.170) indicated normal cerebrovascular function, which was significantly different from the other states (CRx = -0.127 ± 0.061, p = 0.009).</p><p><strong>Discussion: </strong>The study demonstrated that CRx has potential to reflect cerebrovascular function dynamics and assess optimal CPP, demonstrating the potential of bio-electromagnetic technology as a noninvasive indicator for monitoring cerebrovascular function.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1564510"},"PeriodicalIF":4.3,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olfactory mucosal mesenchymal stem cells delivered by gelatin sponge scaffolds promote functional recovery of spinal cord injury.","authors":"Wenshui Li, Xinchen Jiang, Shuo Lu, Wen Lu, Shanshan Ma, Yi Zhuo, Qingtao Gao, Yi Xiao, Binqian Wu, Junyang Xie, Yuhang Yu, Xiangxin Li, Que Deng, Ming Lu","doi":"10.3389/fbioe.2025.1628758","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1628758","url":null,"abstract":"<p><p>Spinal cord injury (SCI) is a pathological condition that damages the central nervous system. Due to the persistence of neuroinflammation after injury, the prognosis is often poor. Recent studies have found that local transplantation of mesenchymal stem cells (MSCs) can improve SCI. However, MSCs retain and engraft at the injured site limit, which may be the reason their effectiveness is greatly reduced. A gelatin sponge (GS), commonly used in clinical practice, was selected as a scaffold to deliver olfactory mucosal mesenchymal stem cells (OM-MSCs). This was done to to enhance local reparative of MSCs at the injury site. We also paid special attention to the biocompatibility of GS co-cultured with OM-MSCs <i>in vitro</i>, and then applied acellular GS and GS loaded with OM-MSCs to the rat SCI model, respectively. After the scaffold was transplanted into rat complete spinal cord injury, behavioral scores and hindlimb movement scores were improved evidently. Local inflammation in the spinal cords of transplanted rats was reduced, and the changes were related to cell pyroptosis. In addition, we found that gelatin sponges and OM-MSC transplantation did not damage other organs in rats. In conclusion, the GS scaffold loaded with OM-MSCs can reduce the local inflammatory microenvironment and facilitate neurological recovery, providing a potential and practical strategy for therapeutic approach of spinal cord injury.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1628758"},"PeriodicalIF":4.3,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electrochemical aptasensor for detection of <i>Helicobacter pylori</i> based on AuNPs and AgNPs-GO nanoparticles.","authors":"Xiaojuan You, Mingyi Shao, Huadong Wang, Rui Zhu, Xinwei Liu, Lei Dong, Yuesheng Gong, Yongwei Li","doi":"10.3389/fbioe.2025.1619336","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1619336","url":null,"abstract":"<p><p>Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of <i>H. pylori</i> is of great significance for the prevention of gastric cancer. Herein, a sensitive electrochemical aptasensor using AgNPs-GO as redox probes was established for the specific detection of <i>H. pylori</i> in blood serum and stool samples. Firstly, AgNPs-GO nanocomposites were obtained by <i>in-situ</i> reduction of AgNPs on graphene oxide (GO) surface with glucose as reducing agent, in which AgNPs showed good biocompatibility and chemical stability, as well as redox property, and GO provided a large surface area to assemble a large number of AgNPs. Subsequently, the electrodeposition of AuNPs further improved the conductivity of the aptasensor. Additionally, the streptavidin was introduced into the aptasensor to effectively bind the biotin-modified aptamers. In this way, aptamers could be tethered to the surface through SA-biotin linkage, and <i>H. pylori</i> was selectivly binded by the aptamers subsequently. Under optimal conditions, the aptasensor could detect <i>H. pylori</i> in a wide concentration range (10¹ CFU mL^-1-10⁸ CFU mL^-1) with a low detection limit of 3 CFU mL^-1. What's more, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other pathogens in clinical diagnosis.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1619336"},"PeriodicalIF":4.3,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent biosensors for human movement rehabilitation and intention recognition.","authors":"Mehrab Rafiq, Nouf Abdullah Almujally, Asaad Algarni, Mohammed Alshehri, Yahya AlQahtani, Ahmad Jalal, Hui Liu","doi":"10.3389/fbioe.2025.1558529","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1558529","url":null,"abstract":"<p><strong>Introduction: </strong>Advancements in sensing technologies have enabled the integration of inertial sensors, such as accelerometers and gyroscopes, into everyday devices like smartphones and wearables. These sensors, initially intended to enhance device functionality, are now pivotal in applications such as Human Locomotion Recognition (HLR), with relevance in sports, healthcare, rehabilitation, and context-aware systems. This study presents a robust system for accurately recognizing human movement and localization characteristics using sensor data.</p><p><strong>Methods: </strong>Two datasets were used: the Extrasensory dataset and the KU-HAR dataset. The Extrasensory dataset includes multimodal sensor data (IMU, GPS, and audio) from 60 participants, while the KU-HAR dataset provides accelerometer and gyroscope data from 90 participants performing 18 distinct activities. Raw sensor signals were first denoised using a second-order Butterworth filter, and segmentation was performed using Hamming windows. Feature extraction included Skewness, Energy, Kurtosis, Linear Prediction Cepstral Coefficients (LPCC), and Dynamic Time Warping (DTW) for locomotion, as well as Step Count and Step Length for localization. Yeo-Johnson power transformation was employed to optimize the extracted features.</p><p><strong>Results: </strong>The proposed system achieved 90% accuracy on the Extrasensory dataset and 91% on the KU-HAR dataset. These results surpass the performance of several existing state-of-the-art methods. Statistical analysis and additional testing confirmed the robustness and generalization capabilities of the model across both datasets.</p><p><strong>Discussion: </strong>The developed system demonstrates strong performance in recognizing human locomotion and localization across different sensor environments, even when dealing with noisy data. Its effectiveness in real-world scenarios highlights its potential for integration into healthcare monitoring, physical rehabilitation, and intelligent wearable systems. The model's scalability and high accuracy support its applicability for deployment on embedded platforms in future implementations.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1558529"},"PeriodicalIF":4.3,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lung-resident SARS-CoV-2 peptide-specific immune responses in perfused 3D human lung explant models.","authors":"Kayla F Goliwas, Anthony M Wood, Christopher S Simmons, Rabisa Khan, Saad A Khan, Yong Wang, Rekha Ramachandran, Joel L Berry, Mohammad Athar, James A Mobley, Young-Il Kim, Victor J Thannickal, Kevin S Harrod, James M Donahue, Jessy S Deshane","doi":"10.3389/fbioe.2025.1587080","DOIUrl":"10.3389/fbioe.2025.1587080","url":null,"abstract":"<p><strong>Introduction: </strong>Multi-specific and long-lasting T-cell immunity has been recognized to indicate long-term protection against pathogens, including the novel coronavirus, SARS-CoV-2, which is the causative agent of the COVID-19 pandemic. Functional significance of peripheral memory T cells in individuals recovered from COVID-19 (COVID-19⁺) is beginning to be appreciated; however, the role of lung tissue-resident memory (lung TRM) T cells in SARS-CoV-2 infection is still being investigated. This is, in part, due to the lack of preclinical tissue models available to follow the convalescence period.</p><p><strong>Methods: </strong>Here, we utilize a perfused three-dimensional (3D) human lung-tissue model and show pre-existing local T-cell immunity against SARS-CoV-2 proteins in lung tissues.</p><p><strong>Results: </strong>We report <i>ex vivo</i> maintenance of functional multi-specific IFN-γ-secreting lung TRM T cells in COVID-19⁺ and their induction in lung tissues of vaccinated COVID-19⁺ subjects. Importantly, we identify SARS-CoV-2 peptide-responding memory B cells and IgA⁺ plasma cells in <i>ex vivo</i> cultured lung tissues of COVID-19⁺. Furthermore, lung tissue IgA levels were increased in COVID-19⁺ and responded to peptide stimulation.</p><p><strong>Discussion: </strong>In our study, we highlight the importance of utilization of human lung-tissue models to understand the local antiviral immune response in the lung to protect against SARS-CoV-2 infection.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1587080"},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Nanodrug delivery strategies for enhanced cancer chemo-immunotherapy.","authors":"Yufen Xiao","doi":"10.3389/fbioe.2025.1653425","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1653425","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1653425"},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical evaluation of percutaneous cement discoplasty combined with percutaneous vertebroplasty: a finite element analysis.","authors":"Xu Zhou, Shenghua Qin, Chunhai Huang, Feiwen Li, Jin Liu, Ti Wu, Mingzheng Zhang","doi":"10.3389/fbioe.2025.1606709","DOIUrl":"10.3389/fbioe.2025.1606709","url":null,"abstract":"<p><strong>Introduction: </strong>Since the introduction of percutaneous cement discoplasty (PCD), numerous studies have confirmed its clinical efficacy in elderly patients. However, PCD is also associated with risks such as bone cement leakage and vertebral fractures. The purpose of this study was to present a biomechanical evaluation of two modified versions of PCD performed in combination with percutaneous vertebroplasty (PVP).</p><p><strong>Methods: </strong>Data from a CT scan of a healthy male's lumbosacral region were used to establish finite element (FE) models of nonsurgical treatment, PCD, L4/5PCD + L4L5PVP (modified technique 1, where the bone cement in the L4/5 disc space does not connect with the L4 and L5 vertebrae) and PCIF (modified technique 2, where the bone cement in the L4/5 disc space connects with the L4 and L5 vertebrae). A compressive of preload 150 N and a moment of 10 N·m were applied to recreate flexion, extension, lateral bending, and axial rotation. The range of motion (ROM) of L3/4 and L4/5, maximum stress on the L3 inferior endplate, L4 inferior endplate and L5 superior endplate, stress on the annulus fibrosus of L4/5, and displacement of the bone cement were evaluated.</p><p><strong>Results: </strong>Both modified techniques outperformed the simple PCD technique in reducing stress on the endplate, stress on the annulus fibrosus, and displacement of the bone cement. The L4/5PCD + L4L5PVP technique was more advantageous in terms of reducing the incidence of postoperative complications. The addition of the PVP technique significantly enhanced spinal stability by increasing support to adjacent vertebrae, thereby reducing the risk of postoperative endplate fractures and bone cement leakage.</p><p><strong>Conclusion: </strong>Modified PCD combined with PVP may be a safer and more effective option for treating degenerative disc diseases, providing important references for clinical treatment.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1606709"},"PeriodicalIF":4.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144689701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Harnessing nanotechnology for cancer treatment.","authors":"Merlis P Alvarez-Berrios, Chuang Liu, Jingjing Sun","doi":"10.3389/fbioe.2025.1651124","DOIUrl":"10.3389/fbioe.2025.1651124","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1651124"},"PeriodicalIF":4.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the molecular mechanisms of the \"Astragalus-Codonopsis\" herb pair in treating diabetes: a network pharmacology and bioinformatics approach with molecular docking validation.","authors":"Jinliang Yang, Mingyang Li, Ziyue Zhu, Fengling Han, Yanyan Ma, Jinbo Hou, Qingfeng Zhao, Hui Yuan, XiuMei Li","doi":"10.3389/fbioe.2025.1618575","DOIUrl":"10.3389/fbioe.2025.1618575","url":null,"abstract":"<p><p>Astragalus membranaceus and Codonopsis pilosula are widely used in traditional chinese medicine for the treatment of diabetes because of their notable hypoglycemic pharmacological effects. Studies have indicatedthat the active compounds in the Astragalus-Codonopsis herb pair may exert their hypoglycemic effects through the modulation of the insulin receptor (IRSP) signaling pathway. In this study, the rhamnolitrin and folic acid were confirmed as the key active components in the Astragalus-Codonopsis herb pair that regulate the IRSP, with their synergistic mechanisms in Type 2 Diabetes Mellitus (T2DM) being further systematically explored by network pharmacology combined with DFT theoretical calculation, molecular docking, molecular dynamics simulation and alanine scanning mutation technology. The results suggest that GSK3β is a critical target through which rhamnolitrin and folic acid exert their anti-diabetic effects. Subsequent molecular docking and molecular dynamics simulations confirmed that both active compounds selected in this study can bind stably with the GSK3β protein. Further alanine scanning mutagenesis experiments validated the importance of key amino acid residues in ligand-receptor interactions. Finally, DFT theoretical calculations provided a detailed elucidation of the binding mechanism between the core components (rhamnolitrin and folic acid) and the target protein GSK3β. This study not only revealed the molecular mechanism of Astragalus-Codonopsis for the treatment of type 2 diabetes, provided a theoretical basis for its clinical application, but also provided a potential molecular target for the development of new anti-diabetes drugs.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1618575"},"PeriodicalIF":4.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing 3D cell models and high-resolution imaging to unveil the mechanisms of nanoparticle-mediated drug delivery.","authors":"Alannah S Chalkley, Maëva T Lopez, Margaritha M Mysior, Madeleen C Brink, Suainibhe Kelly, Jeremy C Simpson","doi":"10.3389/fbioe.2025.1606573","DOIUrl":"10.3389/fbioe.2025.1606573","url":null,"abstract":"<p><p>Nanoparticles and nanosized materials offer huge potential in the field of drug delivery. One key aspect that dictates their successful development is the need to understand how they interact with cells at both the macro and molecular level. Delineating such interactions is vital if nanomaterials are to be targeted not only to particular organs and tissues, but also to individual cell types and ultimately specific subcellular locations. In this regard, the development of appropriate <i>in vitro</i> cell models is an essential prerequisite before animal and human trials. In recent years, as the methodology for their growth has been refined, there has been a huge expansion in the use of pre-clinical 3D cell culture models, particularly spheroids and organoids. These models are attractive because they can be combined with high-resolution fluorescence imaging to provide real-time information on how nanomaterials interact with cells. Confocal fluorescence microscopy and its associated modalities, along with high-content screening and analysis, are powerful techniques that allow researchers the possibility of extracting spatial and temporal information at multiple levels from cells and entire 3D assemblies. In this review, we summarise the state of this field, paying particular emphasis to how imaging of such models is now beginning to provide rich quantitative data about nanomaterial entry and trafficking in cells growing in 3D. We also offer a perspective on the challenges faced by such approaches, and the important questions that the drug delivery field still needs to address.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1606573"},"PeriodicalIF":4.3,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12277333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}