Frontiers in Bioengineering and Biotechnology最新文献

{"title":"The application of dental mesenchymal stem cells (DMSCs) in the repair of oral and maxillofacial defects.","authors":"Yiqi Chen, Xikun Ma, Huaqing Mai, Pengyu Lai, Renlong Huang, Adili Alimujiang","doi":"10.3389/fbioe.2025.1677422","DOIUrl":"10.3389/fbioe.2025.1677422","url":null,"abstract":"<p><p>Dental mesenchymal stem cells (DMSCs) are important seed cells for oral and maxillofacial tissue regeneration due to their wide availability, multi-directional differentiation potential, strong proliferation ability, maintenance of stemness after multiple proliferations, and immunomodulatory capacity. DMSCs have a wide range of sources, and different subtypes have different properties. They can promote tissue regeneration through direct differentiation into tissue cells, improvement of the local regeneration microenvironment through immunomodulatory functions, and release of various bioactive components. The article reviews the classification of DMSCs, their performance comparisons in recent years, methods of promoting tissue regeneration, and their applications in maxillofacial bone and soft tissue. Furthermore, the review explores DMSCs' excellent abilities to promote bone tissue repair when supported by various materials and repair maxillofacial soft tissue defects by promoting the regeneration of special structures of teeth such as dentin, cementum, and dental pulp vessels and nerves.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1677422"},"PeriodicalIF":4.8,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12531237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L5 spondylolysis increases segmental mobility at the cranial adjacent level without altering intervertebral disc contact pressure.","authors":"Zhilin Ge, Bingde Zhao, Xu Xu, Lin Chen, Dongzhu Liang, Qingyang Kang, Zibo Gao, Junhua Luo, Jiheng Zhan, Jianquan Chen, Bo Zhang","doi":"10.3389/fbioe.2025.1653918","DOIUrl":"10.3389/fbioe.2025.1653918","url":null,"abstract":"<p><strong>Objective: </strong>While lumbar spondylolysis has been biomechanically associated with subsequent spondylolisthesis and disc degeneration, its implications on cranial adjacent segments remain unclear. This <i>in vitro</i> experiment aims to quantify the segmental alterations in kinematics and contact mechanics at both L5/S1 and L4/L5 levels induced by L5 pars defects.</p><p><strong>Methods: </strong>Six fresh-frozen human lumbar cadaveric specimens (L1-S2) underwent pure moment loading (4 Nm) in flexion-extension, lateral bending, and axial rotation. Sequential testing compared intact specimens with simulated L5 bilateral spondylolysis models. Intervertebral kinematics were quantified using optical motion tracking, while L4/L5 disc contact parameters were measured using Tekscan pressure sensors.</p><p><strong>Results: </strong>L5/S1 segmental mobility increased in lateral bending (1.66°, p = 0.002) and axial rotation (1.45°, p = 0.007) in spondylolysis models. Motion increases were observed at the cranial adjacent L4/L5 segment: flexion-extension (1.89°, p < 0.001), lateral bending (2.15°, p = 0.002), and axial rotation (1.89°, p = 0.022). However, no significant differences were detected in the L4/L5 disc contact parameters for peak contact pressure, contact area, and contact force.</p><p><strong>Conclusion: </strong>Isthmic defects induce segmental hypermobility at the cranial adjacent segment. This kinematic alteration may accelerate disc degeneration.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1653918"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12529100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanotransduction of autotransplants: remodeling potential of heart valves from autologous pericardial tissue.","authors":"Marvin Steitz, Mahamuda Badhon Khan, Alexander Breitenstein-Attach, Boris Warnack, Frank Edelmann, Felix Berger, Boris Schmitt","doi":"10.3389/fbioe.2025.1680107","DOIUrl":"10.3389/fbioe.2025.1680107","url":null,"abstract":"<p><p>Current commercial heart valve prostheses are non-living structures, either derived from artificial materials (mechanical valves) or foreign biological materials (xeno- or homo-graft). Since the use of viable tissue with native-like properties is essential for a heart valve with self-regulation properties, autologous collagen-based tissue can be considered a promising alternative material. While the extracellular matrix of pericardial tissue offers a solid foundation, it is the interstitial cells that play a crucial role in ensuring long-term durability. This review explores the mechanotransduction capabilities of autologous tissue as a replacement material for living heart valves with regenerative potential.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1680107"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12528101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Nanotechnology-based devices and systems for enhanced sensitivity and efficiency in biomedical applications.","authors":"Donato Conteduca, Casper Kunstmann-Olsen, Steven D Quinn","doi":"10.3389/fbioe.2025.1688121","DOIUrl":"10.3389/fbioe.2025.1688121","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1688121"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12528177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep learning on brain metastasis for predicting EGFR genotype and EGFR-TKI therapy response in metastatic NSCLC: a multicenter study.","authors":"Shuailin You, Ying Fan, Zhiguang Yang, Chunna Yang, Yiyao Sun, Yahong Luo, Zekun Wang, Bo Sun, Wenyan Jiang","doi":"10.3389/fbioe.2025.1637095","DOIUrl":"10.3389/fbioe.2025.1637095","url":null,"abstract":"<p><strong>Background: </strong>Brain metastases are common in patients with advanced non-small cell lung cancer (NSCLC), particularly those harboring EGFR mutations, and accurate prediction of EGFR mutation status and therapeutic response is crucial for guiding targeted therapy. This study aims to conduct a deep learning (DL) approach to automatically predict epidermal growth factor receptor (EGFR) genotype and response to EGFR-tyrosine kinase inhibitor (TKI) therapy in NSCLC patients with brain metastatic tumor (BM).</p><p><strong>Methods: </strong>For training and validating the DL models, 388 patients were enrolled from three centers between Jul. 2014 and Dec.2022 (230 from center 1, 80 from center 2 and 78 from center 3). Contrast-enhanced T1-weighted (T1CE) and T2-weighted (T2W) brain MRI images before treatment for each patient were obtained for analyses. We developed an EGFR-TKI system (ETS) for automated detection of brain metastatic (BM) lesions and to differentiate EGFR mutation status and predict response to EGFR-TKI therapy. The models underwent rigorous evaluation through receiver operating characteristic (ROC) curve analyses, where metrics such as area under the curve (AUC), sensitivity, and specificity were examined.</p><p><strong>Results: </strong>For prediction of EGFR mutation status, the ETS integrating radiological-based features and clinical factors achieved AUCs of 0.842, 0.833 and 0.832 on the internal validation, external validation 1 and external validation 2 cohort, respectively. For forecasting response to EGFR-TKI therapy, the fusion model created by amalgamating MRI with clinical factors generated AUCs of 0.747, 0.726 and 0.728 on the internal validation, external validation 1, and external validation 2 cohort, respectively.</p><p><strong>Conclusion: </strong>The ETS may have the potential to work as a non-invasive tool for predicting EGFR mutation status and response to EGFR-TKI therapy, which holds promise as a non-invasive tool to assist clinicians in making decisions about personalized treatment strategies.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1637095"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12528153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of external load magnitude and carriage techniques on dynamic stability and work performance during fatigued rescue sprint tasks in firefighters.","authors":"Enmeng Jiang, Yan Shi, Xinxin Zhang, Weiguo Liu","doi":"10.3389/fbioe.2025.1671642","DOIUrl":"10.3389/fbioe.2025.1671642","url":null,"abstract":"<p><strong>Background: </strong>This study aims to investigate the effects of different external load magnitudes and carrying techniques on dynamic stability and work performance during fatigued rescue sprint tasks in firefighters, thereby providing theoretical and practical support for injury prevention and performance enhancement in firefighting rescues.</p><p><strong>Methods: </strong>A total of 126 professional healthy male firefighters performed 30-m rescue sprints under simulated fatigue in a 3 × 3 two-factor experimental design combining three external load magnitudes (10 kg, 20 kg, 30 kg) and three carrying techniques (shoulder-carry, bosom-carry, hand-carry).</p><p><strong>Results: </strong>External load magnitude had no significant effect on margin of stability in the medial and lateral directions (MoS_ml) (P > 0.05), but the margin of stability in the anterior and posterior directions (MoS_ap) was significantly lower under 10 kg load tasks compared to 20 and 30 kg loads (P < 0.05). The hand-carry technique significantly increased MoS_ap (P < 0.05) but significantly reduced MoS_ml (P < 0.05). For 20 kg loads, hand-carrying resulted in significantly increased MoS_ml compared to 10 and 30 kg tasks (P < 0.05), while bosom-carrying with 10 kg loads or shoulder-carrying with 20 kg loads significantly reduced MoS_ml (P < 0.05). Center of mass (CoM) work was significantly higher under 20 kg load tasks (P < 0.05). It was also significantly higher when using the hand-carry technique (P < 0.05), particularly when hand-carrying 20 kg loads (P < 0.05). Both external load magnitude and carrying technique significantly affected hip joint work (P < 0.05). Hip joint work was significantly higher under 10 kg loads (P < 0.05), and significantly greater when using hand-carrying compared to bosom-carrying (P < 0.05).</p><p><strong>Conclusion: </strong>It is recommended that firefighters choose carrying techniques based on specific load conditions: avoiding hand-carrying moderate loads, while shoulder carry for moderate to large loads, and bosom carry for small loads. Additionally, hip joint function training should be emphasised in daily routines to improve body control, reduce injury risk during rescue tasks, and enhance overall task performance.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1671642"},"PeriodicalIF":4.8,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12528044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical comparison of the anterior reverse PHILOS and locking compression plate extra-articular distal humerus plates for extra-articular distal humeral fractures.","authors":"Apipop Kritsaneephaiboon, Sujin Wanchat, Thammaphong Khongkanin, Atichart Kwanyuang, Satta Srewaradachpisal, Kantapon Dissaneewate, Wich Orapiriyakul","doi":"10.3389/fbioe.2025.1672989","DOIUrl":"10.3389/fbioe.2025.1672989","url":null,"abstract":"<p><strong>Background: </strong>The locking compression plate extra-articular distal humeral plate (EADHP) is an anatomically pre-contoured plate that is used for extra-articular distal humeral fractures. However, there is currently no standard criterion for the internal fixation of this type of fracture. Moreover, the anterior reverse proximal humeral internal locking system (PHILOS) plate (ARPP) has been clinically applied as a new internal-fixation plate without testing in biomechanical studies. We aimed to compare the biomechanical properties of ARPP and EADHP for the definitive fixation of extra-articular distal humeral fractures.</p><p><strong>Methods: </strong>Eighteen composite humerus bones were cut at the distal humerus using an electrical saw to generate a fracture gap. Internal fixation via the ARPP or EADHP was performed following standard techniques. An Instron testing machine (Instron 8872) was used to evaluate biomechanical properties by applying bending torque, axial force, and torsional torque.</p><p><strong>Results: </strong>Fixations with both ARPP and EADHP could withstand forces that exceeded the physiological forces (200 N). Under axial compression, ARPP constructs demonstrated greater stiffness (668.9 ± 120.7 N/mm vs 171.2 ± 45.4 N/mm) and higher maximal load-to-failure (2,092.6 ± 305.2 N vs 907.0 ± 56.5 N) compared with EADHP, although these differences were not statistically significant. During anterior bending, ARPP provided significantly higher stiffness (17.8 ± 2.0 N/mm vs 13.9 ± 1.0 N/mm, <i>p</i> = 0.041), whereas EADHP showed a higher but non-significant load-to-failure. Under torsional loading, ARPP tended to exhibit greater stiffness in both external and internal rotation, as well as higher load-to-failure (31.1 ± 0.8 N m vs 26.0 ± 4.4 N m), but without statistical significance.</p><p><strong>Conclusion: </strong>ARPP demonstrated superior bending stiffness compared with the EADHP, while both constructs performed equivalently in axial compression and torsion. Therefore, ARPP can serve as an alternative internal-fixation method for extra-articular distal humeral fractures.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1672989"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of platelet-rich plasma injection on anterior cruciate ligament reconstruction: a systematic review and meta-analysis.","authors":"Yiran Zhang, Zhikang Xiao, Zhe Fan, Yingxin Zhang, Jianzhong Xu, Kun Wang","doi":"10.3389/fbioe.2025.1625271","DOIUrl":"10.3389/fbioe.2025.1625271","url":null,"abstract":"<p><strong>Purpose: </strong>This systematic review and meta-analysis evaluates platelet-rich plasma (PRP) efficacy in anterior cruciate ligament reconstruction (ACLR) through 15-year Randomized controlled trials (RCTs) data, focusing on postoperative recovery, rehabilitation acceleration, and functional outcomes optimization.</p><p><strong>Methods: </strong>We conducted an extensive systematic search in PubMed, Embase, and Web of Science to find relevant studies on using PRP in ACLR. Randomized controlled trials analyzing the comparative effectiveness of PRP compared to control interventions in individuals undergoing ACLR were systematically identified. The focus was on studies that provided reliable outcome measures, encompassing validated clinical assessments and objective imaging results. Outcome indicators included the Visual Analog Scale (VAS) for pain perception, the International Knee Documentation Committee (IKDC) score, Lysholm score, Tegner activity scale, KT-1000 side-to-side difference, graft characteristics, and associated complications. Additionally, subgroup analyses were categorized based on evaluation timelines, distinguishing between preoperative and postoperative assessments.</p><p><strong>Results: </strong>This meta-analysis of 24 studies demonstrated time-dependent effects of PRP supplementation following ACLR. The PRP group exhibited significant improvement in IKDC scores at 12 months post-operatively (mean difference: 2.09, P = 0.01, I² = 23%), while Lysholm scores showed significant enhancement at 6 months (mean difference: 3.33, P = 0.03, I² = 58%). Pain reduction, assessed by VAS scores, was significantly greater in the PRP group at 3 months (mean difference: -1.33, P < 0.01, I² = 38%) with borderline significance at 6 months (mean difference: -0.78, P = 0.05). Notably, PRP intervention significantly reduced anterior tibial translation compared to controls (mean difference: -1.34 mm, 95% CI: -1.56 to -1.13, P < 0.01, I² = 73%), indicating improved knee stability. Pre-operative KT-1000 measurements suggested a trend toward reduced knee laxity in the PRP group (mean difference: -0.70 mm, 95% CI: -1.45 to 0.05, P = 0.07), though this effect did not persist post-operatively. No significant between-group differences were observed in Tegner activity scores, Signal-to-Noise Quotient, or Pivot Shift Test results at any follow-up interval.</p><p><strong>Conclusion: </strong>This meta-analysis indicates that PRP application during and shortly after ACLR offers limited clinical benefits. Although there is notable short-term pain relief, long-term efficacy remains unclear, with improvements not meeting minimal clinically important differences (MCID) and no significant changes in knee stability or graft maturation. Further research is needed to establish optimal PRP protocols and standardization for ACLR.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1625271"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism and application of injectable hydrogel as carrier system in the treatment of osteoarthritis.","authors":"Bing-Gang Zhang, Qiang Liu, Tao Ma, Jian-Jun Liu, Yan Zhang, Fang Liu, Xiao-Ming Wen, Duo-Xian Wang, Wei Jiang, Wen-Bo An","doi":"10.3389/fbioe.2025.1636518","DOIUrl":"10.3389/fbioe.2025.1636518","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a disabling degenerative disease that affects synovial joints and leads to cartilage degeneration, which can cause progressive joint damage, chronic pain and functional loss. Because its specific pathogenesis is still unclear, conventional treatment methods are still difficult to achieve satisfactory therapeutic effects. Therefore, finding alternative new methods for treating OA remains a formidable challenge. Hydrogel is a kind of polymer material with good biocompatibility and biodegradability, and it is a new method for the treatment of osteoarthritis. Injectable hydrogel drug delivery platforms have shown many advantages in the treatment of OA, including improved biocompatibility, biodegradability, and low immunogenicity. Injectable hydrogels, as delivery systems, can deliver drugs to the joint cavity in a controlled manner and continuously release them, enhancing drug loading capacity and increasing sensitivity to improve therapeutic effects. This article summarizes the types of injectable hydrogels, analyzes their application as delivery systems in OA, and discusses the mechanisms of injectable hydrogels in the treatment of OA, such as anti-inflammation, anti-oxidative stress, and promotion of articular cartilage regeneration. Meanwhile, the deficiencies of injectable hydrogel drug delivery platforms in the OA field were summarized, and the future research directions in this field were discussed. Overall, injectable hydrogel drug delivery platforms show great potential in the treatment of OA. These innovative methods have brought new hope for the future treatment of OA and pointed out the direction for clinical application.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1636518"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing cell therapy manufacturing: an image-based solution for accurate confluency estimation.","authors":"John Mason, Martin Kraft, Jakob Hueckels, Hao Wei, Konstantinos Spetsieris","doi":"10.3389/fbioe.2025.1651144","DOIUrl":"10.3389/fbioe.2025.1651144","url":null,"abstract":"<p><p>Cell therapies represent a transformative approach for treating diseases resistant to conventional therapies, yet their development and manufacturing face significant hurdles within the biopharmaceutical sector. A critical parameter in the production of these therapies is cell confluency, which serves as both an indicator of biomass in adherent cultures and a determinant of product quality. However, existing methods for measuring confluency are often inadequate for the large-scale cultivation systems used in industry, and current software solutions lack comprehensive automation capabilities necessary for a manufacturing environment. This article introduces a novel image-based software application designed for accurate cell confluency estimation, integrated with a high-throughput microscopy system. Utilizing a machine-learning model for pixel classification, the application facilitates efficient image and metadata processing in a cloud environment, delivering results through an interactive web interface. By incorporating methods from process analytical technologies, manufacturing data digitalization, and data science, this platform enables automated image acquisition, storage, analysis, and reporting in near-real time. The proposed solution aims to streamline the manufacturing process of cell therapeutics, ultimately enhancing the reliability and speed of delivering these innovative treatments to patients.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1651144"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}