Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Leveraging spatial dependencies and multi-scale features for automated knee injury detection on MRI diagnosis.","authors":"Jianhua Sun, Ye Cao, Ying Zhou, Baoqiao Qi","doi":"10.3389/fbioe.2025.1590962","DOIUrl":"10.3389/fbioe.2025.1590962","url":null,"abstract":"<p><strong>Background: </strong>The application of deep learning techniques in medical image analysis has shown great potential in assisting clinical diagnosis. This study focuses on the development and evaluation of deep learning models for the classification of knee joint injuries using Magnetic Resonance Imaging (MRI) data. The research aims to provide an efficient and reliable tool for clinicians to aid in the diagnosis of knee joint disorders, particularly focusing on Anterior Cruciate Ligament (ACL) tears.</p><p><strong>Methods: </strong>KneeXNet leverages the power of graph convolutional networks (GCNs) to capture the intricate spatial dependencies and hierarchical features in knee MRI scans. The proposed model consists of three main components: a graph construction module, graph convolutional layers, and a multi-scale feature fusion module. Additionally, a contrastive learning scheme is employed to enhance the model's discriminative power and robustness. The MRNet dataset, consisting of knee MRI scans from 1,370 patients, is used to train and validate KneeXNet.</p><p><strong>Results: </strong>The performance of KneeXNet is evaluated using the Area Under the Receiver Operating Characteristic Curve (AUC) metric and compared to state-of-the-art methods, including traditional machine learning approaches and deep learning models. KneeXNet consistently outperforms the competing methods, achieving AUC scores of 0.985, 0.972, and 0.968 for the detection of knee joint abnormalities, ACL tears, and meniscal tears, respectively. The cross-dataset evaluation further validates the generalization ability of KneeXNet, maintaining its superior performance on an independent dataset.</p><p><strong>Application: </strong>To facilitate the clinical application of KneeXNet, a user-friendly web interface is developed using the Django framework. This interface allows users to upload MRI scans, view diagnostic results, and interact with the system seamlessly. The integration of Grad-CAM visualizations enhances the interpretability of KneeXNet, enabling radiologists to understand and validate the model's decision-making process.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1590962"},"PeriodicalIF":4.3,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12088959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterials reshape the pulmonary mechanical microenvironment: novel therapeutic strategies for respiratory diseases.","authors":"Li-Zhen Chen, Peng-Fei Zheng, Qi Cai, Run-Nan Chen","doi":"10.3389/fbioe.2025.1597387","DOIUrl":"10.3389/fbioe.2025.1597387","url":null,"abstract":"<p><p>Respiratory diseases, including chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and lung cancer, exhibit elevated death rates and pathological intricacy, requiring advancements that surpass the constraints of traditional therapies. This study comprehensively outlines the novel applications of nanomaterials in respiratory medicine by accurately modulating the pulmonary mechanical microenvironment, encompassing alveolar surface tension, extracellular matrix rigidity, and the immune-fibroblast interaction network. The precise delivery, stimuli-responsive characteristics, and biomimetic design of nanomaterials markedly improve drug concentration at the lesion site and mitigate fibrosis, inflammation, and malignant tumor advancement by disrupting mechanical signaling pathways. The study clarifies their multifaceted benefits in treating COPD, IPF, and lung cancer, including decreased systemic toxicity and improved spatiotemporal control. Nonetheless, clinical translation continues to encounter obstacles, including impediments in large-scale production, inadequate compatibility with breathing devices, and disputes concerning long-term biosafety. In the future, the amalgamation of precision medicine, adaptive smart materials, and multi-omics artificial intelligence technologies will facilitate the development of individualized diagnostic and therapeutic systems, establishing a novel paradigm for the proactive management of respiratory disorders. This review offers essential theoretical foundations and technical approaches for the practical application of nanomaterials and the enhancement of therapeutic techniques in respiratory medicine.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1597387"},"PeriodicalIF":4.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights on poly(<i>cis</i>-1,4-isoprene) rubber degradation through enzymatic kinetics and process improvement.","authors":"Camila Guajardo-Flores, Josefa Rojas, Yvan Baldera-Moreno, Francisco Adasme-Carreño, Daisuke Kasai, Rodrigo Andler","doi":"10.3389/fbioe.2025.1593339","DOIUrl":"10.3389/fbioe.2025.1593339","url":null,"abstract":"<p><p>Latex clearing protein (Lcp) is a crucial enzyme in the oxidative degradation of poly(<i>cis</i>-1,4-isoprene), the main component of natural rubber (NR). Despite significant biochemical advances, to date, the kinetic behavior of Lcp from <i>Streptomyces</i> sp. K30 (Lcp_K30) has not been characterized, limiting the efficiency of NR conversion. In this work, Lcp_K30 was produced in <i>Escherichia coli</i> BL21 (DE3) + p4782.1::<i>lcp</i> _K30 with L-rhamnose as the inducer, yielding 6.05 mg/L of purified protein. Kinetic assays demonstrated a positive correlation between the initial reaction rate and poly(<i>cis</i>-1,4-isoprene) concentration, reaching a maximum rate of 7.05 nmol O₂/min at the enzyme's saturation point, corresponding to 5 μg Lcp/mg NR. The Michaelis-Menten constant (K_m) was determined to be 308.3 mg/mL, with the Hill model providing the best fit for the data. NR-to-oligoisoprenoid conversion reached 12.9 mg in 24 h, exceeding previously reported yields, while gel permeation chromatography analysis indicated conversion efficiencies over 80%, far exceeding the reports of previous studies where only 30%-40% conversions were achieved. Furthermore, Fukui function analysis suggested that the aldehyde terminal groups of the oligoisoprenoids may be less susceptible to enzymatic degradation, which would explain the mass distribution of the degradation products.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1593339"},"PeriodicalIF":4.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supramolecular nanofibers of natural asiaticoside for self-supporting gelation and enhanced transdermal delivery.","authors":"Xixi Hu, Shuang Tian, Jiao Wang, Weixi Luo, Jiangli Yao, Rui Zhu, Yiyuan Dai, Hongyun Li, Yuhua Ma, Chen Liu, Wenping Wang","doi":"10.3389/fbioe.2025.1589865","DOIUrl":"10.3389/fbioe.2025.1589865","url":null,"abstract":"<p><strong>Objectives: </strong>The study aimed to develop a supramolecular hydrogel of asiaticoside (AS) via self-assembly and evaluate its potential for enhanced transdermal delivery.</p><p><strong>Materials and methods: </strong>AS was dissolved in dimethyl sulfoxide (DMSO) and dispersed into a glycerol-water mixture (3:7 v/v) via ultrasonication to induce gelation. The critical gelation concentration (CGC) was determined through macroscopic and microscopic evaluation. Morphological analysis was performed using various microscopy techniques. Physicochemical properties were assessed using differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), Fourier-transform infrared (FTIR) spectroscopy, and UV-VIS spectroscopy. Molecular dynamics (MD) simulations with general AMBER force field (GAFF) parameters were used to analyze assembly dynamics. Rheological behavior and transdermal performance were tested using a rheometer and Franz diffusion cells, respectively.</p><p><strong>Results: </strong>The hydrogel formed at a CGC of 0.5% w/v, exhibiting pH-responsive gelation and a nanofibrous architecture. MD simulations revealed hydrogen bonding and π-π stacking as the dominant drivers of assembly, supported by FTIR peak shifts. The hydrogel demonstrated shear-thinning behavior (G' > G″) and thermal stability below 70°C. Compared to the AS suspension, the hydrogel enhanced transdermal flux by 1.73-fold and skin retention by 2.04-fold, attributed to supersaturated drug molecules and sustained release from the nanofiber network.</p><p><strong>Conclusion: </strong>This work pioneers the use of AS as a natural supramolecular gelator, addressing its bioavailability challenges through nanostructured self-assembly. The hydrogel's dual functionality (pH-responsive gelation and enhanced permeation) offers a sustainable platform for the transdermal delivery of hydrophobic phytochemicals, bridging phytochemistry and nanobiotechnology. This strategy expands the application of plant-derived saponins in advanced drug delivery systems.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1589865"},"PeriodicalIF":4.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of SWATH-MS methods for measurement of residual host cell proteins in adeno-associated virus preparations.","authors":"Thomas M Leibiger, Lie Min, Kelvin H Lee","doi":"10.3389/fbioe.2025.1579098","DOIUrl":"10.3389/fbioe.2025.1579098","url":null,"abstract":"<p><strong>Introduction: </strong>Analysis of residual host cell proteins in adeno-associated virus (AAV) preparations is challenging due to low availability and high complexity of samples. One strategy to address these challenges is through development of improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods with greater sensitivity and reduced sample requirement.</p><p><strong>Methods: </strong>In this work, we compare the performance of four sequential window acquisition of all theoretical fragment ion mass spectra (SWATH-MS) methods for identification and quantitation of residual HCPs in rAAV2, -5, -8, and -9 preparations produced with human embryonic kidney 293 (HEK293) cells and purified using immunoaffinity chromatography. Key SWATH-MS parameters including spectral library construction (data dependent vs. <i>in silico</i>), data processing software (DIA-NN vs. Skyline), and mass spectrometer instrument (Sciex TripleTOF 6600 vs. Sciex ZenoTOF 7600) were assessed. Method attributes including sample requirement and processing time, and method outputs including protein and precursor identifications, host cell protein quantitation comparisons across methods, and quantitation coefficients of variance (CV) were considered to help establish a SWATH-MS workflow well-suited for rAAV HCP analytics.</p><p><strong>Results: </strong>A 78% increase in HCP identifications, 80% reduction in sample requirement, and 70% reduction in instrument runtime was achieved with an <i>in silico</i> spectral library, data processing in DIA-NN, and data collection with the Sciex ZenoTOF 7600 instrument (DIA-NN-7600 method) compared to a previously established method using a DDA-derived spectral library, data processing in Skyline, and data collection with the Sciex TripleTOF 6600 instrument (Skyline-DDA-6600 method). Additionally, the DIA-NN-7600 method shows median HCP quantitation CV below 10% for triplicate data acquisitions, and comparable quantitation to other methods for a panel of highly abundant residual HCPs previously identified in rAAV downstream processing.</p><p><strong>Discussion: </strong>This work highlights a SWATH-MS method with data collection and processing specifically tailored for rAAV residual HCP analysis.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1579098"},"PeriodicalIF":4.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoarchitecting intelligently encapsulated designs for improved cancer therapy.","authors":"Ying-Tong Ye, Hong-Ying Xia, Jie Li, Shi-Bin Wang, Ai-Zheng Chen, Ranjith Kumar Kankala","doi":"10.3389/fbioe.2025.1587178","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1587178","url":null,"abstract":"<p><p>Despite the success in exploring various aspects of origination and therapeutic strategies, cancer has remained one of the most dreadful metabolic disorders due to failure to eradicate tumors comprehensively and frequent recurrence because of acquired resistance to the drugs. Recently, several advancements have been evidenced in the fabrication of various smart nanocarriers encapsulated with multiple components. Several reasons for smart nanoencapsulation include the enhancement of the bioavailability of drugs, precise targetability to reduce adverse effects on normal cells, and the ability to enable controlled drug release rates at the tumor sites. In addition, these smart nanocarriers protect encapsulated therapeutic cargo from deactivation, responsively delivering it based on the physiological or pathological characteristics of tumors. In this review, we present various smart approaches for cancer therapy, including organic materials, inorganic components, and their composites, as well as biomembrane-based nanoencapsulation strategies. These nanoencapsulation strategies, along with practical applications and their potential in cancer treatment, are discussed in depth, highlighting advantages and disadvantages, as well as aiming to reveal the ultimate prospects of nanoencapsulation in enhancing drug delivery efficiency and targeted cancer therapy.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1587178"},"PeriodicalIF":4.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering extracellular matrix-based hydrogels for intervertebral disc regeneration.","authors":"Mwafaq Kmail, Rusydi Razak, Isma Liza Mohd Isa","doi":"10.3389/fbioe.2025.1601154","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1601154","url":null,"abstract":"<p><p>Lower back pain (LBP) is a major health concern, especially in older adults. A key aetiological factor is intervertebral disc (IVD) degeneration. It is mediated by dysregulation of extracellular matrix (ECM) and inflammation. In recent years, regenerative therapies have garnered attention for their potential to restore disc function by addressing the underlying biological alterations within the IVD. This review focuses on the comprehensive understanding of the anatomy and physiology of the IVD, highlighting its life cycle from embryonic development, and maturation to degenerative phenotype. We describe current treatments for managing LBP caused by IVD degeneration. This review emphasizes on the recent advancements in hydrogel engineering, highlighting natural, synthetic, and composite hydrogels and their application in ECM-targeted regenerative therapy for IVD degeneration. By exploring innovations in hydrogel technology, including improvements in crosslinking techniques and controlled degradation rates-we discuss how these materials could enhance IVD regeneration and potentially be used for the management of LBP. With their enhanced biomimicry, hydrogel-based ECM mimics offer a promising pathway for developing effective, durable therapies that address the root causes of disc degeneration, providing new hope for individuals living with chronic LBP.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1601154"},"PeriodicalIF":4.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studying the effects of McRoberts and neonate-focused maneuvers on the neonatal brachial plexus during shoulder dystocia.","authors":"Joy A Iaconianni, Rania Bakhri, Bernard Gonik, Sriram Balasubramanian, Anita Singh","doi":"10.3389/fbioe.2025.1474154","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1474154","url":null,"abstract":"<p><p>This study investigates the effects of clinical delivery maneuvers on neonatal brachial plexus (BP) during complicated birthing scenarios such as shoulder dystocia. Shoulder dystocia occurs when the anterior shoulder of the neonate is obstructed behind the maternal symphysis pubis and prevents the delivery of the neonate. Maneuvers such as McRoberts, application of suprapubic pressure (SPP), oblique positioning, and posterior arm delivery are performed sequentially to alleviate the obstruction. This study used MADYMO, a computer software program, to simulate these maneuvers during shoulder dystocia while maternal endogenous forces (82N and 129N) were applied. The recorded outcomes were the magnitude of neonatal BP stretch during delivery and the amount of clinician-applied traction (CAT) force, if required, to achieve delivery. The lithotomy position was treated as the baseline and compared to the McRoberts position, at 82N and 129N maternal forces. Additionally, in McRoberts position, at 82N and 129N maternal forces, neonate-focused maneuvers were applied, and the clinician applied traction (CAT) force, if required, to achieve delivery was recorded along with the resulting neonatal BP stretch. The simulations, at 82N maternal force, reported a decrease in required CAT force in the McRoberts position compared to the lithotomy position. The results of the neonate-focused maneuvers reported a further decrease in the CAT force and the resulting BP stretch. Furthermore, increasing SPP from 40N to 100N reported no required CAT force for delivery along with decreased BP stretch. Oblique positioning further decreased the BP stretch, and the posterior arm delivery of the neonate resulted in the least amount of BP stretch. No CAT forces were required during these maneuvers. The simulations, at 129N maternal force, reported similar trends of reduced BP stretch during delivery except no CAT forces were required during any simulated conditions. Findings from this study help understand the effects of McRoberts position and neonate-focused maneuvers on neonatal brachial plexus during complicated shoulder dystocia delivery. The reported required delivery forces, both maternal and CAT also lay the groundwork for clinician training and education while guiding the development of preventative approaches that can limit neonatal injuries.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1474154"},"PeriodicalIF":4.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond clinical scales: an observational study on instrumental gait analysis and biomechanical patterns in patients with Parkinson's disease.","authors":"Paolo De Pasquale, Mirjam Bonanno, Cristiano De Marchis, Luca Pergolizzi, Antonino Lombardo Facciale, Giuseppe Paladina, Maria Grazia Maggio, Federica Impellizzeri, Irene Ciancarelli, Angelo Quartarone, Rocco Salvatore Calabrò","doi":"10.3389/fbioe.2025.1541240","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1541240","url":null,"abstract":"<p><strong>Introduction: </strong>Parkinson's disease (PD), a common neurodegenerative disorder affecting motor functions, is associated with abnormal gait patterns characterized by altered kinematic, kinetic, and electrophysiological parameters. This observational study aims to instrumentally identify and quantify these gait dysfunctions in PD patients compared to normal values from healthy subjects.</p><p><strong>Methods: </strong>Sixty-nine PD patients underwent clinical and instrumental evaluations to assess gait. Demographic and clinical data were collected before motor assessment. Clinical scales evaluated the level of impairment, gait, balance, risk of falls and ability to complete activities of daily living. Instrumental evaluations were conducted using optoelectronic, force plates and electromyographic (EMG) systems in a motion analysis laboratory. Statistical analysis involved a non-parametric test to compare pathological and normal data, clustering methods to identify groups based on clinical evaluations, and a combination of non-parametric analysis and linear models to assess dependencies on clinical scales.</p><p><strong>Results: </strong>The results showed that PD patients had significant gait kinematic differences compared to normal values, with increased temporal and shortened spatial parameters. In addition, PD patients were grouped into four clusters based on clinical scales. While some gait features were influenced by clinical scales reflecting impairment, gait and balance, and independence, others were more affected by the perceived fear of falling (FoF).</p><p><strong>Discussion: </strong>In conclusion, the study identified specific biomechanical gait dysfunctions in kinematic, kinetic, and electrophysiological parameters in PD patients, undetectable by standard clinical scales. Additionally, higher FoF was associated with dysfunctional biomechanical patterns, independent of impairment severity, gait and balance dysfunction, or overall independence.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1541240"},"PeriodicalIF":4.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intensification of 2'-Fucosyllactose biosynthesis pathway by using a novel fucosyltransferase from <i>Bacillus cereus</i>.","authors":"Kainuo Zhang, Miaomiao Gao, Chenqi Cao, Mengxin Zhang, Waqar Ahmad, Ahmed Rady, Badr Aldahmash, Tianze Zhu, Shahin Shah Khan, Luo Liu","doi":"10.3389/fbioe.2025.1569597","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1569597","url":null,"abstract":"<p><strong>Introduction: </strong>2'-Fucosyllactose (2'-FL) is an oligosaccharide that can be synthesized in the human body and is known for its health-promoting and prebiotic effects. The biosynthesis of 2'-FL using microorganisms has received attention recently due to its increased application in nutritional and medical infant formulations.</p><p><strong>Methods: </strong>This work attempts the new application of <i>Bacillus cereus</i> α-1,2-fucosyltransferase (FutCB) in the <i>de novo</i> synthesis of 2'-FL in <i>Escherichia coli</i> (<i>E. coli</i>). Additionally, knocking out the <i>LacZ</i> and <i>WaaF</i> genes alongside overexpression of the key gmd, manB, wcaG, and manC genes enhances the availability of the necessary precursors GDP-L-fucose and lactose for the synthesis of 2'-FL.</p><p><strong>Results and discussion: </strong>The use of constitutive promoters achieved better control over the production of 2'-FL during fed-batch fermentation. After 64 h of fermentation, the modified <i>E. coli</i> strains produced 121.4 g/L 2'-FL with a yield of 1.90 g/L/h, resulting in an impressive 2'-FL output. These results together indicate the potential of large-scale, high-yield production of 2'-FL and form a basis of much more refinement to be done. The next step will focus on maximum substrate utilization, alteration of gene regulation, and improvement of commercial-scale synthesis.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1569597"},"PeriodicalIF":4.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}