Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Recombinant humanized collagen combined with nicotinamide increases the expression level of rat basement membrane proteins and promotes hair growth.","authors":"Youshiqi Zhou, Boyu Chen, Shijia Ye, Haoyu Sun, Shuyue Wang, Xiaozhen Diao, Wenhui Wu","doi":"10.3389/fbioe.2025.1546779","DOIUrl":"10.3389/fbioe.2025.1546779","url":null,"abstract":"<p><strong>Background: </strong>Hair follicle stem cells (HFSCs) play crucial roles in hair growth and are expected to be potential targets in regenerative medicine and tissue engineering.</p><p><strong>Method: </strong>This study aims to investigate the positive effect on hair growth by the recombinant human collagen complex (RHC complex), composed of rhCOL III, rhCOL XVII, and rhCOL XXI, along with nicotinamide, both <i>in vitro</i> and <i>in vivo</i>, by HFSCs and rat models. The survival rate, function, and differentiation of HFSCs were investigated.</p><p><strong>Results: </strong>The CCK-8 experiment showed that the RHC complex was non-toxic to HFSCs, and the cell survival rate exceeded 80% after 8 and 16 h of treatment. The ELISA method showed that the RHC complex significantly increased the intracellular vascular endothelial growth factor (VEGF) levels. In addition, the increase in the content of trichohyalin (a key structural protein of hair) indicates that the structure and function of hair follicles may be enhanced. The expression levels of β-integrin and p63 were significantly upregulated, which are crucial for cell adhesion, migration, and maintenance of HFSCs homeostasis. In the rat model, significant hair growth was observed after a 7-day treatment period. The period of vigorous hair growth in rats was selected for immunofluorescence, enzyme-linked immunosorbent assay (ELISA) and hematoxylin-eosin (HE) staining analysis. The results showed that the RHC complex could promote the expression of Integrin, Laminin and Perlecan, which were conducive to maintaining the stability of the microenvironment of HFSCs. Additionally it facilitated the migration and differentiation of HFSCs, as evidenced by an increased number of hair follicles in HE-stained skin tissues. In conclusion, the RHC complex exhibited high HFSCs survival rates and enhanced the expression of HFSCs-associated factors and basement membrane proteins. These properties make the RHC complex a promising novel ingredient for promoting hair growth, preventing hair loss, and maintaining hair health.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1546779"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pluripotency genes of mammals: a network at work.","authors":"Ranieri Cancedda, Maddalena Mastrogiacomo","doi":"10.3389/fbioe.2025.1578499","DOIUrl":"10.3389/fbioe.2025.1578499","url":null,"abstract":"<p><p>Pluripotency, i.e., the ability to differentiate into cells of all three germ layers, is a transient state of early embryonic cells. In mammals, during progression from pre-implantation to post-implantation stage, pluripotent cells undergo different state transitions characterized by changes in gene expression and development potential. These developmental states include: (i) a naive pluripotency (pre-implantation embryonic stem cells, or ESCs), (ii) an intermediate condition (formative state), and (iii) a primed pluripotency (late post-implantation ESCs derived from epiblasts also named EpiSCs). The transitions are regulated by an interconnected network of pluripotency-related genes. Transcription of genes such as <i>Oct4, Sox2</i>, and <i>Nanog</i> is crucial for obtaining and maintaining pluripotency. These three factors form an autoregulatory loop by binding to each other's promoters to activate their transcription. Other factors play a significant ancillary role in the transcription factor network preserving cell pluripotency. In the review, we will also mention some of the more relevant cytokines, molecules, signaling pathways, and epigenetic modifications that induce and control pluripotency gene expression. The main goal of this review is to bridge the gap between the fields of genetics and stem cell biology and to set the ground for the application of this knowledge to the development of strategies and drugs to be used in a clinical environment.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1578499"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Proteomic analysis of silk fibroin reveals diverse biological function of different degumming processing from different origin.","authors":"Yaling Wang, Yunyun Liang, Jiacen Huang, Yisheng Gao, Zhixin Xu, Xuejun Ni, Yumin Yang, Xiaoming Yang, Yahong Zhao","doi":"10.3389/fbioe.2025.1612862","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1612862","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fbioe.2021.777320.].</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1612862"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel.","authors":"Yong Qi, Shuyun Zhang, Yanni He, Shuanji Ou, Yang Yang, Yudun Qu, Jiaxuan Li, Wanmin Lian, Guitao Li, Junzhang Tian, Changpeng Xu","doi":"10.3389/fbioe.2025.1636750","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1636750","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fbioe.2022.1070566.].</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1636750"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12199717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tunable methacrylated decellularized heart matrix: a versatile scaffold for cardiac tissue engineering.","authors":"Valinteshley Pierre, Douglas H Wu, Chao Liu, Elif Ertugral, Chandrasekhar Kothapalli, Samuel E Senyo","doi":"10.3389/fbioe.2025.1579246","DOIUrl":"10.3389/fbioe.2025.1579246","url":null,"abstract":"<p><p>Therapeutic tissue regeneration remains a significant unmet need in heart failure and cardiovascular disease treatment, which are among the leading causes of death globally. Decellularized heart matrix (DHM) offer promising advantages for tissue engineering, including low immunogenicity and seamless integration into biological processes, facilitating biocompatibility. However, DHM is challenged by weak mechanical properties that limit its utility to biomedical applications like tissue engineering. To address this limitation, we functionalized DHM with methacryloyl functional groups (DHMMA) that support UV-induced crosslinking to enhance mechanical properties. By modulating the degree of methacryloyl substitution, a broad range of stiffness was achieved while maintaining cell viability on crosslinked DHMMA. Additionally, we show that increasing UV exposure time and pH increases DHMMA stiffness. Furthermore, topographical features transferred on DHMMA via soft lithography facilitated physical orientation of cells in culture. We demonstrate DHMMA as a scaffold with tunable stiffness and matrix-degradation properties suitable for cell survival and microfabrication for cardiac tissue engineering applications.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1579246"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methods for processing and analyzing images of vascularized micro-organ and tumor systems.","authors":"Stephanie J Hachey, Christopher J Hatch, Daniela Gaebler, Alexander G Forsythe, Makena L Ewald, Alexander L Chopra, Zhangying Chen, Kapil Thapa, Melvin Hodanu, Jennifer S Fang, Christopher C W Hughes","doi":"10.3389/fbioe.2025.1585003","DOIUrl":"10.3389/fbioe.2025.1585003","url":null,"abstract":"<p><p>Our group has developed and validated an advanced microfluidic platform to improve preclinical modeling of healthy and disease states, enabling extended culture and detailed analysis of tissue-engineered miniaturized organ constructs, or \"organs-on-chips.\" Within this system, diverse cell types self-organize into perfused microvascular networks under dynamic flow within tissue chambers, effectively mimicking the structure and function of native tissues. This setup facilitates physiological intravascular delivery of nutrients, immune cells, and therapeutic agents, and creates a realistic microenvironment to study cellular interactions and tissue responses. Known as the vascularized micro-organ (VMO), this adaptable platform can be customized to represent various organ systems or tumors, forming a vascularized micro-tumor (VMT) for cancer studies. The VMO/VMT system closely simulates <i>in vivo</i> nutrient exchange and drug delivery within a 3D microenvironment, establishing a high-fidelity model for drug screening and mechanistic studies in vascular biology, cancer, and organ-specific pathologies. Furthermore, the optical transparency of the device supports high-resolution, real-time imaging of fluorescently labeled cells and molecules within the tissue construct, providing key insights into drug responses, cell interactions, and dynamic processes such as epithelial-mesenchymal transition. To manage the extensive imaging data generated, we created standardized, high-throughput workflows for image analysis. This manuscript presents our image processing and analysis pipeline, utilizing a suite of tools in Fiji/ImageJ to streamline data extraction from the VMO/VMT model, substantially reducing manual processing time. Additionally, we demonstrate how these tools can be adapted for analyzing imaging data from traditional <i>in vitro</i> models and microphysiological systems developed by other researchers.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1585003"},"PeriodicalIF":4.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finite element analysis-assisted surgical planning and evaluation of flap design in hand surgery.","authors":"Guang Yang, Hui Shen, Yewon Jang, Xiangyi Cheng","doi":"10.3389/fbioe.2025.1611993","DOIUrl":"10.3389/fbioe.2025.1611993","url":null,"abstract":"<p><p>Given the anatomical variability among patients and the intricate geometry of the hand, the shape and size of the skin flap have traditionally relied heavily on the surgeon's experience and subjective judgment. This dependence can lead to inconsistent and sometimes suboptimal results, particularly in complex cases such as web reconstruction in syndactyly surgery. Finite element analysis (FEA) provides a quantitative method to simulate and optimize skin flap design during surgery. However, existing FEA studies in this field are scattered across a wide range of seemingly unrelated topics. To address this, we present a comprehensive review focused on the application of FEA in skin flap design since 2000, with attention to all aspects of preprocessing and postprocessing. The primary objective is to evaluate the potential of FEA to generate patient-specific models by integrating individualized anatomical and biomechanical data while identifying key advancements, analyzing methodological challenges, exploring emerging technologies, and outlining future research directions. A critical finding is that the mechanical modeling of skin remains a major limitation in current FEA applications. To address this, future studies should focus on the development and refinement of non-invasive techniques for acquiring patient-specific skin properties. We also recommend several additional research directions based on our findings. These include exploring techniques to unfold 3D wound surfaces into 2D representations, which can improve mesh quality and computational efficiency; validating FEA simulations through large-scale, multicenter clinical studies to ensure robustness and generalizability; developing real-time AR/MR systems that integrate simulation or optimization results into surgical workflows; and creating AI-powered platforms that learn from clinical data to provide adaptive and personalized flap design recommendations. These findings offer a pathway to bridge the gap between simulation and clinical practice, ultimately aiming to improve surgical outcomes.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1611993"},"PeriodicalIF":4.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive photoelectrochemical immunosensor based on floral cluster SnS₂/ZnCdS heterostructure for the detection of CA199.","authors":"Hui Zhou, Qingqing Guo, Xin Zhang, Tingting Chu, Wen Zhang, Qing Liu, Linlin Cao","doi":"10.3389/fbioe.2025.1584456","DOIUrl":"10.3389/fbioe.2025.1584456","url":null,"abstract":"<p><p>The early and accurate detection of tumor markers is crucial for cancer diagnosis, prognosis, and treatment monitoring. Carbohydrate antigen 199 (CA199), as a key biomarker of pancreatic, gastric, and colorectal cancers, is widely used in the clinical management. The development of sensitive, rapid and cost-effective detection methods for CA199 is of paramount importance in improving early detection rates and patient outcomes. In this study, we present a novel photoelectrochemical (PEC) immunosensor based on a SnS₂/ZnCdS heterostructure designed for the ultrasensitive detection of CA199. The unique heterojunction between SnS₂ and ZnCdS enhances photocurrent generation by effectively suppressing charge recombination and improving charge separation. Furthermore, the flower-like morphology of the heterostructure further boosts light absorption and photogenerated carrier transport, resulting in significantly enhanced sensor performance. This label-free PEC immunosensor exhibits outstanding stability, reproducibility and selectivity, with a broad detection range from 0.01 to 1000 U/mL and an ultra-low detection limit of 1.00 × 10^-3 U/mL. These features demonstrate the potential of this sensor as a powerful tool for sensitive CA199 detection, offering promising applications in cancer diagnostics and monitoring.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1584456"},"PeriodicalIF":4.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Applications of digital twin technology in dentistry.","authors":"Sandipan Roy, Raphaël Richert, João Manuel R S Tavares, Pierre Lahoud","doi":"10.3389/fbioe.2025.1624734","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1624734","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1624734"},"PeriodicalIF":4.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a global forearm reconstruction system for post-tumor resection defects of the radius or ulna: a proof-of-concept study.","authors":"Haijie Liang, Jie Zang, Siyi Huang, Boyang Wang, Shun Tang, Zhiye Du, Feiyang Qi, Wei Guo, Jichuan Wang, Xiaodong Tang","doi":"10.3389/fbioe.2025.1547652","DOIUrl":"10.3389/fbioe.2025.1547652","url":null,"abstract":"<p><strong>Background: </strong>Bone defects resulting from sarcoma resection in the forearm present significant challenges for reconstruction, with limited guidance available in the literature.</p><p><strong>Methods: </strong>We developed a novel series of 3D-printed endoprostheses, called the Global Forearm Reconstruction System (GFRS), to reconstruct defects of the proximal radius (PR), distal ulna (DU), total ulna (TU), and total radius (TR). Finite element analysis (FEA) was performed to determine the mechanical support function of the GFRS endoprostheses. We also tested the rotatory function of the endoprostheses <i>ex vivo</i> using a resin model. Finally, we summarized the preliminary outcomes of three pediatric cases using the GFRS endoprostheses for reconstruction.</p><p><strong>Results: </strong>Resection of PR, DU, TU and TR leads to stress concentration in the remaining structures, which can be mitigated by the corresponding GFRS endoprostheses. The novel endoprostheses demonstrated full supination capability and approximately 50% of pronation in the <i>ex vivo</i> model. All of the three clinical cases achieved satisfactory functional status (MSTS-93:28-29; MEPS: 95-100) without complications during mid-term follow-up (32-42 months).</p><p><strong>Conclusion: </strong>In this proof-of-concept study, we demonstrated that the GFRS endoprostheses not only meet the theoretical reconstruction requirements but also exhibit a good safety profile and produce satisfactory functional outcomes in a preliminary cohort with mid-term follow-up.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1547652"},"PeriodicalIF":4.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}