Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Computational simulations of endocrine bone diseases related to pathological glandular PTH secretion using a multi-scale bone cell population model.","authors":"Corinna Modiz, Natalia M Castoldi, Stefan Scheiner, Javier Martínez-Reina, Jose L Calvo-Gallego, Vittorio Sansalone, Saulo Martelli, Peter Pivonka","doi":"10.3389/fbioe.2025.1619276","DOIUrl":"10.3389/fbioe.2025.1619276","url":null,"abstract":"<p><strong>Introduction: </strong>Bone diseases significantly impact global health by compromising skeletal integrity and quality of life. In disease states linked to parathyroid hormone (PTH) glandular secretion, disrupted PTH patterns typically promote osteoclast proliferation, leading to increased bone resorption.</p><p><strong>Methods: </strong>While mathematical modeling has proven valuable in analyzing bone remodeling, current bone cell population models oversimplify PTH secretion by assuming constant levels, limiting their ability to represent disorders characterized by variations in PTH pulse characteristics. To address this, we present a novel semi-coupled approach integrating a two-state PTH receptor model with an established bone cell population model. Instead of conventional Hill-type functions, we implement a cellular activity function derived from the receptor model, incorporating pulsatile PTH patterns, cell dynamics, and intracellular communication pathways.</p><p><strong>Results: </strong>Our numerical simulations demonstrate the model's capability to reproduce various catabolic bone diseases, providing realistic changes in bone volume fraction over a 1-year period. Notably, while direct implementation of PTH disease progression in the bone cell population model fails to capture diseases only characterized by altered pulse duration and baseline, such as glucocorticoid-induced osteoporosis, our semi-coupled approach successfully models these conditions.</p><p><strong>Discussion: </strong>This physiologically more realistic approach to endocrine disease modeling offers potential implications for optimizing therapeutic interventions and understanding disease progression mechanisms.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1619276"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism design and human-machine coupling analysis for a lumbar rehabilitation robot.","authors":"Yuan Tian, Fengping Chen, Zixu Zhao, Dawei Jiang, Wenjing Ji, Jiasheng Dai","doi":"10.3389/fbioe.2025.1678755","DOIUrl":"10.3389/fbioe.2025.1678755","url":null,"abstract":"<p><strong>Introduction: </strong>Superman Exercise is highly recommended by healthcare professionals for patients with low back pain. However, performing this treatment can be challenging due to patients' difficulty in maintaining the training maneuvers and the lack of standardization in these maneuvers.</p><p><strong>Methods and procedures: </strong>A low back rehabilitation robot, designed based on swallow movement rehabilitation training, was fully modeled for patients with low back motor dysfunction. The robot underwent kinematic analysis, including forward and inverse solutions, as well as kinetic analysis to verify its accuracy. By studying human waist mobility and muscle force, a human-machine coupling model was developed to determine the range of human waist joint angles and muscle force size. This was achieved by importing three-dimensional motion capture data into the OpenSim human motion analysis environment and comparing it with the human body's natural lumbar rehabilitation movements. Additionally, comparisons were made with the human body during lumbar rehabilitation training maneuvers.</p><p><strong>Conclusion: </strong>The rehabilitation robot is well-designed to meet patients' rehabilitation training needs and uncover the rehabilitation patterns of the human waist. This study serves as a reference for future parameter optimization and control system design.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1678755"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly imprinted polymer/CdTe quantum dots-decorated polymer optic fiber microprobes for Amoxicillin detection.","authors":"Jianfeng Liang, Qixuan Wu, Ran Xiao, Ruijie Yu, Hao Chen, Yangjie Tang, Jie Zhang, Chenchen Liu, Guowei Yang, Hongxiang Lei","doi":"10.3389/fbioe.2025.1700654","DOIUrl":"10.3389/fbioe.2025.1700654","url":null,"abstract":"<p><p>Amoxicillin (AMX) is a widely used antibiotic for infectious diseases. However, excessive residues of AMX in the food chain and environment pose serious threats to public health, making precise monitoring of AMX crucial. Among various detection methods, fluorescence spectroscopy has garnered significant attention due to its unique advantages. Nevertheless, conventional fluorescence probes based on organic dyes or quantum dots (QDs) suffer from limitations such as difficult separation, easy pollution, poor biocompatibility and safety, lack of specificity and <i>in situ</i> detection. To address these challenges, we developed a novel sensor based on a molecularly imprinted polymer (MIP)/CdTe quantum dots-decorated polymer optical fiber microprobe (POF MP) for AMX detection. This sensor offers multiple advantages, including good specificity, reusability and stability, excellent biocompatibility and safety, <i>in situ</i> monitoring, and residue-free operation. The proposed sensor demonstrates a linear detection range of 0.5-50 μg/L with a limit of detection (LOD) of 0.31 μg/L. This innovative sensor provides a promising solution for monitoring AMX concentrations in biological and environmental systems, contributing to advancements in microenvironmental monitoring, pharmaceutical sensing, and biomedical therapeutics.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1700654"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Pilot-scale cultivation of the snow alga <i>Chloromonas typhlos</i> in a photobioreactor.","authors":"Floris Schoeters, Jornt Spit, Rahmasari Nur Azizah, Sabine Van Miert","doi":"10.3389/fbioe.2025.1697072","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1697072","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/fbioe.2022.896261.].</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1697072"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12522050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The versatility of algae in addressing the global sustainability challenges.","authors":"Bishnu Dev Das, Ajaya Bhattarai","doi":"10.3389/fbioe.2025.1621817","DOIUrl":"10.3389/fbioe.2025.1621817","url":null,"abstract":"<p><p>Algae have developed into a sustainable and adaptable resource that can help with several global issues, such as resource depletion, environmental degradation, food security, climate change, and energy security. It explores the multifaceted potential of algae in addressing key global sustainability challenges-including climate change, resource depletion, environmental pollution, food insecurity, and energy demands-through biotechnological innovations. Chlorella, Nannochloropsis, Botryococcus, and Spirulina demonstrate exceptional efficiency in biomass production, carbon sequestration, nutrient recycling, and bioenergy generation. Objectives of this review include evaluating recent advances in algal-based wastewater remediation, biodiesel production, and circular bioeconomy strategies, with a focus on the integration of industrial waste streams like abattoir wastewater and crude glycerol. Notably, Chlorella sorokiniana has shown high potential for phycoremediation and biodiesel yield when cultivated in abattoir wastewater digestate (AWD), achieving up to 90% BBM replacement with enhanced lipid and carotenoid content. Similarly, the valorization of crude glycerol via microbial and insect-based systems underscores algae's role in supporting low-carbon bioeconomies. In agriculture, macroalgae such as Asparagopsis taxiformis have significantly reduced enteric methane emissions in livestock, highlighting their utility in climate-smart farming. Despite these advances, the scalability and economic viability of algal technologies remain constrained by high production costs, energy-intensive processing, contamination risks, and regulatory limitations-especially in food and feed sectors. It advocates for targeted research into cost reduction, process optimization, and harmonized policy frameworks to unlock algae's full potential. By addressing these challenges, algae can become central to sustainable development strategies, enabling effective transitions toward cleaner energy, healthier ecosystems, and resilient food systems.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1621817"},"PeriodicalIF":4.8,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinematic and mechanical assessment of seated lumbar rotation manipulation: force, velocity and orientation in three dimensions.","authors":"Changxiao Han, Jiali Chen, Jinghua Gao, Congcong Wen, Haibao Wen, Xunlu Yin, Bochen Peng, Guangwei Liu, Liguo Zhu, Minshan Feng","doi":"10.3389/fbioe.2025.1651760","DOIUrl":"10.3389/fbioe.2025.1651760","url":null,"abstract":"<p><strong>Background: </strong>Seated lumbar rotation manipulation is widely used for low back pain, but lacks detailed biomechanical analysis. Understanding its biomechanical characteristics is crucial for therapists to improve comprehension and support education and research. The purpose of this study was to analyze the kinematic and mechanical parameters of Seated lumbar rotation manipulation.</p><p><strong>Methods: </strong>Sixty healthy volunteers underwent manipulation by experienced therapists. Three-dimensional movements, thrust velocity, and acceleration were measured using motion capture technology. Force parameters were recorded using pressure sensing gloves mounted on the therapist's hands. Subgroup comparisons were conducted based on body mass index, and linear regression was used to analyse the relationship between force parameters and BMI (Body Mass Index). Finally, Pearson's correlation test was employed to examine the correlation between the forces exerted by both hands during each procedure.</p><p><strong>Results: </strong>Kinematic analysis indicated that the angles in three directions were greatest for rotation, followed by lateral bending and flexion. Similarly, rotation was the dominant angular velocities, greater than lateral flexion and anteflexion. Furthermore, Preload duration (2.72 ± 0.10 s) and thrust duration (0.48 ± 0.04 s) were recorded. In terms of force, four key force metrics were calculated: preload force (58.99 ± 9.76 N), valley force (23.25 ± 6.24 N), thrust force (50.54 ± 9.63 N), and peak force (73.77 ± 11.06 N). While the preload rate (21.73 ± 4.66 N/s), thrust rate (106.30 ± 11.72 N/s), and the maximum torque (51.86 ± 7.52 N m) were determined. Subgroup analysis showed significant differences in force parameters by body types (P < 0.01). Linear regression revealed a positive correlation between BMI and force parameters (P < 0.05), and Pearson analysis indicated a significant correlation between forces exerted by both hands (P < 0.05).</p><p><strong>Conclusion: </strong>Seated lumbar rotation manipulation is characterized by long-lever, three-dimensional coupled movements with high-velocity, low-amplitude thrusts. Additionally, the force parameters are positively influenced by somatotype, and bilateral hand force exerts a synergistic effect. This valuable biomechanical quantification help comprehending the technique and supporting its educational and experimental settings.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1651760"},"PeriodicalIF":4.8,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12518278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145299551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterials in the diagnosis and therapy of osteoarthritis: current research advances and future clinical prospects.","authors":"Xin Li, Zeyan Shen, Songou Zhang, Yuke Chen, Xuanyuan Lu, Xujun Hu","doi":"10.3389/fbioe.2025.1664799","DOIUrl":"10.3389/fbioe.2025.1664799","url":null,"abstract":"<p><p>Osteoarthritis (OA), the most prevalent degenerative joint disorder, is characterized by progressive cartilage degradation, synovial inflammation, and functional impairment. Current treatments mainly alleviate symptoms without halting disease progression, and systemic drug administration often leads to poor absorption, short half-life, off-target effects, and adverse reactions. Recent advances in nanotechnology provide innovative solutions through nanomaterials with superior physicochemical properties, enabling targeted delivery, sustained release, and reduced toxicity. Compared with conventional therapies, nanotherapeutic strategies enhance treatment efficacy, support cartilage regeneration, and offer diagnostic potential. This review summarizes recent progress in nanomaterials for OA therapy, including liposomes, polymeric and inorganic nanoparticles, exosomes, gene delivery systems, and multifunctional platforms. We highlight their mechanisms, advantages, limitations, and translational potential, aiming to provide a comprehensive reference for future nanomedicine development in OA treatment.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1664799"},"PeriodicalIF":4.8,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12518342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145299539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different induction methods and post-activation potentiation on lower limb muscle activation and explosive power.","authors":"Xingchen Zhang, Yuan Gao, Yang Sun, Enjing Li","doi":"10.3389/fbioe.2025.1674571","DOIUrl":"10.3389/fbioe.2025.1674571","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the acute effects of a different-intensity resistance warm-up on lower limb isokinetic strength, muscle activation, and exercise performance under blood flow restriction.</p><p><strong>Methods: </strong>Using an isokinetic dynamometer, surface electromyography (sEMG) system, and force platform, lower limb isokinetic strength characteristics, electromyographic parameters, jump kinetics, kinematics, and other relevant parameters were assessed in 15 healthy males following different warm-up induction protocols.</p><p><strong>Results: </strong>Isokinetic strength testing:HBFR produced higher knee extension torque than LLRT at 3,6,12 min (<i>P</i> = 0.012, <i>P</i> = 0.028, <i>P</i> = 0.019) and surpassed LBFR at 9 min (<i>P</i> = 0.015). LBFR increased torque immediately post-warm-up (0 min vs pre: <i>P</i> = 0.049), while HBFR peaked at 3 min (<i>P</i> = 0.040). Jump performance: HBFR achieved greater flight height than LBFR (<i>P</i> = 0.002). At 6 min, LLRT showed lower peak power vs LBFR/HBFR (<i>P</i> = 0.046, <i>P</i> = 0.034). LBFR increased flight height at 3/6 min (<i>P</i> = 0.049, <i>P</i> = 0.045), HBFR at 0/3 min (<i>P</i> = 0.048, <i>P</i> = 0.020). EMG data: LBFR exhibited higher vastus lateralis RMS than HLRT at 9 min (<i>P</i> = 0.035). MPF differed significantly between groups across timepoints (<i>P</i> = 0.031, <i>P</i> = 0.026, <i>P</i> = 0.000, <i>P</i> = 0.047). HBFR increased vastus medialis RMS at 6 min (<i>P</i> = 0.032), while HLRT decreased MPF at 6/12 min (<i>P</i> = 0.019, <i>P</i> = 0.045).</p><p><strong>Conclusion: </strong>HBFR warm-up amplifies regional ischemia by superimposing intrinsic and extrinsic constraints, synergistically enhancing neuromuscular recruitment and metabolic stress. This mechanism sustains elevated force output and potentiates PAP, albeit with elevated load-associated injury risks. LBFR warm-up achieves muscle activation comparable to high-intensity training under reduced mechanical loading. The temporal manifestation of PAP exhibits task-specific variability across performance metrics, necessitating individualized BFR protocol optimization and precise recovery time modulation based on target outcomes. Collectively, LBFR represents an efficacious warm-up strategy with minimized injury risk, as evidenced by the present findings.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1674571"},"PeriodicalIF":4.8,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12518325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145299541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical study of different internal fixation devices for femoral neck fractures: finite element analysis based on different reduction qualities.","authors":"Zhexi Zhu, Zhongjian Tang, Yafei Lu, Tao Yang, Jizhe Lu, Chenglong Pang, Bin Wang","doi":"10.3389/fbioe.2025.1678294","DOIUrl":"10.3389/fbioe.2025.1678294","url":null,"abstract":"<p><strong>Objective: </strong>To compare and analyze the biomechanical advantages and disadvantages of four cannulated screws (4CCS) internal fixation device, biplanar double support screw (BDSF) internal fixation device, and dynamic hip screw (DHS) internal fixation device in anatomical reduction, positive reduction, and negative reduction of femoral neck fractures with a Pauwels angle of 50° using the finite element method.</p><p><strong>Methods: </strong>Based on the finite element method, a femoral neck fracture model with a Pauwels angle of 50° was established. Anatomical reduction, positive reduction, and negative reduction femoral neck fracture models were respectively constructed on this basis. Then, 4CCS, BDSF, and DHS internal fixation devices were implanted into the three types of femoral neck models, resulting in a total of 9 groups of finite element models. Each fracture group was tested under an axial load of 2100 N, and the displacement of the femur and internal fixation devices, as well as the distribution of Von Mises stress (VMS), were measured.</p><p><strong>Results: </strong>In the femoral neck fracture model with a 50° Pauwels angle, maximum femoral displacement in the BDSF group was 3.412 mm for anatomical reduction, 3.459 mm for positive reduction, and 3.962 mm for negative reduction-all smaller than those in the 4CCS and DHS groups. For femoral head stress, the 4CCS group showed higher stress (42.90 Mpa) than BDSF (38.21 Mpa) and DHS (35.91 Mpa) under anatomical reduction. However, under positive and negative reduction, BDSF (23.75 Mpa and 27.9 Mpa) outperformed 4CCS (50.40 Mpa and 34.6 Mpa) and DHS (44.92 Mpa and 44.6 Mpa). Negative reduction models had significantly greater overall stress than positive reduction models, with positive reduction showing better stability. Under anatomical reduction, BDSF's internal fixation stress (222.3 Mpa) and displacement (3.611 mm) differed notably from DHS (322.2 Mpa, 3.009 mm) and 4CCS (276.0 Mpa, 3.346 mm). Under positive and negative reduction, BDSF (247.4 Mpa/3.370 mm and 292.1 Mpa/3.865 mm) performed better than 4CCS (250.4 Mpa/3.480 mm and 293.1 Mpa/3.897 mm). BDSF and 4CCS had significantly lower internal fixation stress than DHS under positive and negative reduction, while no significant difference in internal fixation displacement was found between BDSF and DHS under these conditions.</p><p><strong>Conclusion: </strong>For femoral neck fractures with a Pauwels angle of 50° under anatomical reduction, positive reduction, and negative reduction, the BDSF internal fixation device has better biomechanical performance than the 4CCS and DHS internal fixation devices. Except for anatomical reduction, positive reduction can achieve better biomechanical results. The BDSF internal fixation technique can be considered a reliable closed reduction internal fixation technique for treating femoral neck fractures with different anatomical reductions.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1678294"},"PeriodicalIF":4.8,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12518237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145299501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing the predictive techno-economic and lifecycle assessment of prairie grass and manure co-digestion for renewable natural gas applications.","authors":"Katherine Wild, Juan Carlos Dominguez, Lisa Schulte Moore, Mark Mba Wright","doi":"10.3389/fbioe.2025.1651510","DOIUrl":"10.3389/fbioe.2025.1651510","url":null,"abstract":"<p><p>Prairie grass remains an underutilized agricultural resource that could provide economic, environmental, and ecological benefits to the bioeconomy. Prairie grass and manure anaerobic digestion is a promising pathway for renewable natural gas (RNG) production, but there is limited information on how co-digestion ratios impact RNG performance. This study integrates the Anaerobic Digestion Model No. 1 (ADM1) into a techno-economic analysis (TEA) and life cycle assessment (LCA) framework to evaluate RNG production via co-digestion of prairie biomass and cattle manure. Simulations across eleven feedstock ratios showed that co-digestion can increase methane yields compared to mono-digestion of prairie biomass. The highest methane production rate (227 mL/gVS) and the lowest minimum fuel selling price (MFSP) of $41.88/GJ occurred at a 1:9 prairie-to-manure volatile solids (VS) ratio. RNG yields reached 10.1 GJ/dry tonne for this configuration-39% higher than prairie-only digestion. LCA results revealed that manure-based scenarios had the lowest global warming potential (-16.0 kg CO₂-eq/GJ), while prairie-based scenarios reduced ecotoxicity (-190 kg 2,4-D-eq/GJ). Economic and environmental benefits were further improved by accounting for biochar coproducts via system expansion and allocation. Results underscore the value of ADM1 in optimizing AD system design for both profitability and sustainability.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1651510"},"PeriodicalIF":4.8,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12518338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145299557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}