Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Enhancing caries-affected dentin bonding with a mussel-inspired primer.","authors":"Yuntong Hu, Yi He, Dingjie Wang, Yingjing Wei, Xiaodong Xing, Yuhong Xiao","doi":"10.3389/fbioe.2025.1574562","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1574562","url":null,"abstract":"<p><strong>Introduction: </strong>Dental caries is the most common oral disease. In caries-affected dentin (CAD), excessive mineral loss, extensive collagen exposure and collapse, increased enzyme activity, and bacterial residues result in significantly lower resin bonding strength and durability compared to sound dentin (SD). Currently, there are no effective clinical strategies to enhance CAD bonding. Inspired by the excellent wet adhesion capability and collagen affinity of marine mussels, this study aimed to evaluate a mussel-inspired polymerizable monomer (catechol-Lys-methacrylate [CLM]) as a primer to improve CAD bonding performance.</p><p><strong>Methods: </strong>The interactions between CLM and collagen were analyzed <i>via</i> Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). Microtensile bond strength, nanoleakage, <i>in-situ</i> zymography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to assess the bond strength and interface stability. Furthermore, the antibacterial properties of CLM were evaluated using colony-forming units counts, live/dead bacterial staining, and bacterial morphology observation.</p><p><strong>Results: </strong>FTIR and NMR results showed that CLM was successfully grafted onto CAD collagen through its catechol groups, facilitating subsequent chemical bonding with resin. CLM increased the immediate CAD bond strength by approximately 30% and reduced immediate nanoleakage by approximately 24%, maintaining effectiveness after aging. Moreover, collagen chemical modification by CLM promoted collagen crosslinking, inhibited endogenous enzymatic activity, and conferred antibacterial properties, further enhancing bonding interface stability.</p><p><strong>Discussion: </strong>In summary, this study reports the application of a mussel-inspired monomer, CLM, in CAD bonding. During the wet bonding process, CLM not only improves collagen stability but also serves as a molecular bridge between inorganic resin and organic collagen, thereby enhancing both immediate and aged bonding performance. These findings showing promising clinical application potential.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1574562"},"PeriodicalIF":4.3,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in insulin delivery: the potential of natural polymers for improved diabetes management.","authors":"Mohammed Ghazwani, Umme Hani, Ashishkumar Kyada, Suhas Ballal, Bahjat Saeed Issa, Munthar Kadhim Abosaoda, Abhayveer Singh, A Sabarivani, Subhashree Ray","doi":"10.3389/fbioe.2025.1566743","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1566743","url":null,"abstract":"<p><p>Diabetes is a growing global health issue, with millions of people affected by the condition. While insulin therapy is vital for managing both Type 1 and Type 2 diabetes, traditional methods such as subcutaneous injections have notable drawbacks, including pain, discomfort, and difficulty in maintaining stable blood sugar levels. To improve insulin delivery, research is increasingly focused on the use of natural polymers-substances derived from plants, animals, and microorganisms. These polymers, including materials like alginate, chitosan, and hyaluronic acid, have promising properties such as biocompatibility, biodegradability, and the ability to provide controlled, sustained insulin release. By encapsulating insulin in polymers, it is protected from degradation and released in a manner that more closely mirrors the body's natural insulin production. Furthermore, the development of non-invasive delivery methods, such as oral and transdermal systems, is gaining momentum, offering the potential for more patient-friendly treatment options. This review discusses the role of natural polymers in insulin delivery, examining their mechanisms, types, and current research efforts. It also addresses the challenges that remain in advancing these technologies into practical clinical use, aiming to provide more efficient, comfortable, and effective solutions for diabetes management.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1566743"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive biocompatibility profiling of human pancreas-derived biomaterial.","authors":"Amish Asthana, Amanda Gallego, Quentin Perrier, Tamara Lozano, Lori N Byers, Jun Ho-Heo, Wonwoo Jeong, Riccardo Tamburrini, Arunkumar Rengaraj, Deborah Chaimov, Alice Tomei, Christopher A Fraker, Sang Jin Lee, Giuseppe Orlando","doi":"10.3389/fbioe.2025.1518665","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1518665","url":null,"abstract":"<p><strong>Introduction: </strong>The importance of the extracellular matrix (ECM) to pancreatic islets has been clearly demonstrated, as isolated islets grown in culture or transplanted, quickly lose viability and function after their matrix associations have been stripped away during the isolation process. Therefore, recapitulating the islet niche is a critical objective to move the field of islet transplantation forward.</p><p><strong>Methods: </strong>As a first step to recreating the islet microenvironment, we have recently developed a detergent-free decellularization method to obtain a decellularized solubilized ECM (dsECM) powder from human pancreas. We have also shown that this gentler method (compared to traditional detergent-based methods) allows for thorough preservation of the molecular fingerprint of the innate organ. Furthermore, incorporation of dsECM in alginate-microencapsulated human islets, showed a significant increase in insulin secretion, compared to both free and alginate-only encapsulated islets. However, it is also essential to test the interaction of dsECM with multiple cell types to establish its safety for transplantation.</p><p><strong>Results and discussion: </strong>Herein, we present a comprehensive <i>in vitro</i> evaluation of the cytotoxicity, hemocompatibility and immunocompatibility of dsECM to establish a concentration range where it deemed safe and biocompatible. Furthermore, dsECM-based bioinks were coaxially bioprinted and the resulting construct's biocompatibility and vascularization potential were also evaluated <i>in vivo</i>.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1518665"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Better models, better treatment? a systematic review of current three dimensional (3D) <i>in vitro</i> models for implant-associated infections.","authors":"Neele Brümmer, Katharina Doll-Nikutta, Patrik Schadzek, Carina Mikolai, Andreas Kampmann, Dagmar Wirth, Andrea Hoffmann, Philipp-Cornelius Pott, Oliver Karras, Sören Auer, Meike Stiesch","doi":"10.3389/fbioe.2025.1569211","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1569211","url":null,"abstract":"<p><strong>Introduction: </strong>Understanding the biology of implant-associated infections is essential in order to provide adequate detection, prevention and therapeutic strategies. Advanced 3D in vitro models offer valuable insights into the complex interactions between cells and bacteria in the presence of implant materials. This review aims to give a comprehensive overview of current 3D in vitro models that mimic implant-associated infections.</p><p><strong>Methods: </strong>The structured literature search initially identified 258 publications, seven of which fitted the inclusion criteria.</p><p><strong>Results: </strong>The included 3D models were established either to mimic the in vivo situation (organotypic model) or to investigate future implant materials. In three studies, organotypic models for dental implants were created and one study described an organotypic model containing immune cells. In the remaining three studies, biomaterials for constructing future orthopedic implants were developed and tested. All authors included specific cells and bacteria suitable for the respective implants. The dental implant models used fibroblasts and keratinocytes; the orthopedic implant models used stem cells and fibroblast-like cells; the model containing immune cells incorporated co-cultivation of fibroblasts and THP-1 derived macrophages. For bacterial challenge, most authors used Gram positive bacteria, but three studies employed Gram negative bacterial species. A wide variety of analytical methods of different complexity were applied after co-culture of cells and bacteria and between one and five different methods were used.</p><p><strong>Discussion: </strong>All models could be employed to provide answers to specific scientific questions regarding implant-associated infections. Nonetheless, this review reveals the limitations of current 3D models for the investigation of implant-associated infections and highlights the opportunities for further development in this scientific field.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1569211"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proof-of-concept study of the TriBot: a robot-based test setup for biotribological analyses of curved articular surfaces.","authors":"Luisa de Roy, Moritz Roderigo, Jonas Schwer, Klaus Schlickenrieder, Anita Ignatius, Andreas Martin Seitz","doi":"10.3389/fbioe.2025.1546060","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1546060","url":null,"abstract":"<p><strong>Introduction: </strong>Investigations on the articular cartilage (AC) frictional properties contribute to a better understanding of knee joint functionality. We identified the need for a tribological setup that allows for friction measurements on curved AC surfaces, without disrupting its structural integrity, under orthogonal contact conditions and controlled normal force application. Therefore, a robotic-based tribometer-the TriBot-was developed and validated in a two-part proof-of-concept study.</p><p><strong>Methods: </strong>First, the friction coefficients of polyoxymethylene pins on three different polyamide (PA) shapes were determined for validation purposes. Second, the frictional properties on porcine tibial plateaus were investigated. Trajectories on the medial and the lateral tibial surface were tested in the intact cartilage state and after inducing an anteromedial local defect.</p><p><strong>Results: </strong>No significant differences in the friction coefficients of the PA samples were found. Inducing an anteromedial cartilage defect significantly increased friction on the affected trajectories (+30%, p < 0.05).</p><p><strong>Discussion: </strong>Our findings showed that the robotic tribometer is suitable for friction measurements on complexly shaped samples and that the system can detect differences in cartilage friction due to structural tissue damage. Overall, the robotic tribometer has the potential to advance our understanding of the knee joint's friction-related functionality.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1546060"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thoraco-abdominal biomechanical model and dual-layer control method for soft robotic system with application to respiratory assistance.","authors":"Wenzhuo Zhi, Wei Zhao, Yan Zhang, Enming Shi, Yangfan Zhou, Bi Zhang","doi":"10.3389/fbioe.2025.1581402","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1581402","url":null,"abstract":"<p><strong>Introduction: </strong>Respiratory dysfunction remains a critical challenge for patients transitioning from intensive care. However, existing assistive devices often fail to address both human-robot dynamic synchronization and patient safety due to the lack of interaction force control. Therefore, this study proposes a human-robot force interaction control strategy by integrating a flexible force sensor, aimed at achieving precise alignment between assistive forces and natural respiratory rhythms.</p><p><strong>Methods: </strong>In this study, a wearable respiratory assistive robotic system was developed to provide respiratory assistance by applying compressive force to the user's abdomen through soft origami actuators. A thoracoabdominal biomechanical transmission analysis was conducted to reveal the cross-domain force transfer mechanisms. To improve the human-robot adaptability, a dual-layer control architecture for force-pressure coordinated control was designed. Besides, through hardware integration and system building, along with the implementation of interaction force control, the respiratory assistive robot achieves effective respiratory assistance control.</p><p><strong>Results: </strong>Within the 12-40 breaths/min effective respiratory rate range, PEF, MTV, and MV improved significantly. PEF had a 20.12% average increase, MTV a 19.69% average boost, and MV a 15.5% average rise. Statistically, PEF and MV improvements were highly significant across this range, while MTV was highly significant at 20 breaths/min. Respiratory MV measurements segmented by phase showed that the robot enhanced expiratory function and improved inspiratory function at certain rates within 12-40 breaths/min.</p><p><strong>Discussion: </strong>The proposed human-robot interaction control system integrates hardware and control systems. Tests on healthy subjects in the effective operating range show that the robotic system can enhance subjects' overall respiratory function and ventilation function, offering a technical reference for future respiratory-assist robot development.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1581402"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiles of volatiles in microalgae depend on the extraction and analytical methods.","authors":"Carla C C R de Carvalho, Kilian Neves, Sebastião V T F de Almeida, Pia Steinrücken, Dorinde M M Kleinegris","doi":"10.3389/fbioe.2025.1589776","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1589776","url":null,"abstract":"<p><strong>Introduction: </strong>The incorporation of microalgae in food products is dependent on their volatile profiles, which significantly influence their organoleptic characteristics and, consequently, consumer acceptance. However, microalgae contain a myriad of volatile compounds, and their precise impact on olfactory and gustatory perception is not easily inferred. Nonetheless, volatiles imparting a fish-like odour are generally considered undesirable. To develop enzyme processes or other methods targeting malodour compounds, they must be identified and quantified in the microalgal biomass. However, no standard method allowing the extraction of all volatiles is available.</p><p><strong>Methods: </strong>In the present study, the volatile profiles of the freshwater microalgal species <i>Chlorella vulgaris</i> and <i>Arthrospira platensis</i> and of the marine microalgal species <i>Microchloropsis gaditana</i>, <i>Tetraselmis chui</i>, and <i>Phaeodactylum tricornutum</i> were determined by gas chromatography.</p><p><strong>Results: </strong>A total of five fractions were obtained by sequential elution of increasingly polar solvents and different methods to break the cells were tested. Additionally, the lipid composition of each species was determined and compared.</p><p><strong>Discussion: </strong>The study clearly shows that extraction and analytical methods have a strong impact on the reported volatile and lipid profile of the cells.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1589776"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Bioimaging applications in biosensors and biomolecular electronics.","authors":"Sai Kumar Tammina, Abuelmagd Mahmoud Abdelmonem, Naveen Kumar Reddy Bogireddy","doi":"10.3389/fbioe.2025.1568124","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1568124","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1568124"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Emerging advances in exploiting pulmonary administration for treatment of thoracic diseases.","authors":"Lina Wu, Gregory M Lanza, Jin Xie, Dipanjan Pan","doi":"10.3389/fbioe.2025.1612172","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1612172","url":null,"abstract":"","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1612172"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted and intelligent nano-drug delivery systems for colorectal cancer treatment.","authors":"Meihong Chai, Shihua Wang, Yuxin Chen, Xing Pei, Xueyan Zhen","doi":"10.3389/fbioe.2025.1582659","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1582659","url":null,"abstract":"<p><p>Colorectal cancer (CRC) remains a highly heterogeneous malignancy with significant morbidity and mortality worldwide. Despite advancements in surgery, chemotherapy, immunotherapy, and targeted therapy, treatment efficacy is often hampered by drug resistance and systemic toxicity. In recent years, nano-drug delivery systems (NDDS) have emerged as a promising strategy to enhance therapeutic precision, reduce adverse effects, and overcome resistance in CRC treatment. This review discusses the recent advancements in NDDS for CRC treatment, focusing on the optimization of oral drug delivery systems, the development of tumor-specific targeting strategies, and the design of intelligent delivery systems responsive to the tumor microenvironment (TME). Furthermore, we summarize current challenges in NDDS translation and explore future research directions for enhancing their clinical feasibility and therapeutic impact.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1582659"},"PeriodicalIF":4.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12061940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}