Frontiers in Bioengineering and Biotechnology最新文献

{"title":"More than just life and death: advances in imaging and analysis for 3D-bioprinted tissues.","authors":"Erin R Spiller, Daniela F Duarte Campos","doi":"10.3389/fbioe.2025.1600077","DOIUrl":"10.3389/fbioe.2025.1600077","url":null,"abstract":"<p><p>3D bioprinting is a fast-growing field with applications in both microphysiological systems and tissue engineering. However, the qualifications and definitions of success for 3D-bioprinted products are insufficient. We can further our characterization of 3D-bioprinting methods and finished products using new imaging techniques and analysis methods, including the use of AI tools. This multi-faceted approach can deepen our understanding of valuable technology by examining the effects of 3D bioprinting on cell identity, behavior and organelles. Defining a successful 3D-bioprinted product in addition to viability is crucial in the push toward using these models for drug screening or disease modeling, where robust and high-quality systems are required for meaningful data output.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1600077"},"PeriodicalIF":4.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation and finite element analysis of bone cement-augmented anterolateral screw fixation versus percutaneous bilateral pedicle screw fixation co-applied with oblique lumbar interbody fusion for single-level lumbar degenerative diseases with osteoporosis.","authors":"Xiaoping Mu, Xiaodong Wei, Jiahong Nong, Huabao Ye, Zhuhai Li, Minke Wei, Jianxun Wei","doi":"10.3389/fbioe.2025.1571849","DOIUrl":"10.3389/fbioe.2025.1571849","url":null,"abstract":"<p><strong>Background: </strong>As the population ages, there is an increasing trend in patients with lumbar degenerative diseases (LDD) complicated by osteoporosis seeking lumbar fusion surgery. However, standardized strategies for minimally invasive surgical procedures among these populations still need improvement in clinical practice.</p><p><strong>Purpose: </strong>This study was to integrate clinical and biomechanical approaches to investigate and demonstrate the effectiveness of oblique lateral interbody fusion combined with bone cement-augmented anterolateral screw (OLIF-BCAAS) in such patients.</p><p><strong>Study design: </strong>A single-center, retrospective case-controlled clinical study and finite element model (FEM) analysis.</p><p><strong>Methods: </strong>A single-center, retrospective case-controlled clinical study and finite element model (FEM) analysis were conducted. 48 cases were enrolled in the clinical study, then assigned to either OLIF-BCAAS or OLIF combined with posterior internal fixation with pedicle screws (OLIF-PIFPS). Clinical outcomes and radiological parameters were statistically analyzed. The FE models of intact lumbar spine, OLIF-BCAAS, and OLIF-PIFPS were constructed based on computed tomography (CT) scans of a healthy male. These FE models were analyzed under different loading conditions.</p><p><strong>Results: </strong>There were significant differences in the surgical time, blood loss, and lower back score within 1 year postoperatively between the two groups (p < 0.05). Moreover, both OLIF surgical techniques showed significant improvements in disc height (DH) postoperatively; however, the reduction in DH at postoperative 12 months was more pronounced in the OLIF-PIFPS group than in the OLIF-BCAAS group (p < 0.05). Five cases (5/23, 21.74%) of cage subsidence (CS) were detected in the OLIF-BCAAS group, with 4 out of 23 cases (17.39%) considered as mild CS. In contrast, the amount of CS was 12 cases (12/25, 48%) in the OLIF-PIFPS group, which included 3 cases of severe CS. However, there was a trend towards statistical difference in CS between the two groups (p = 0.057). The FEM analysis showed significant reductions in the local range of motion and L3 maximum displacement with respect to L4 under six motion patterns in the two OLIF surgical models. Moreover, stress on the endplate and cage in the OLIF-BCAAS model was higher than that in the OLIF-PIFPS model; however, stress on the supplemental fixation devices was significantly lower than that observed in the OLIF-PIFPS model.</p><p><strong>Conclusion: </strong>Both OLIF surgical techniques for treating LDD with osteoporosis can achieve favorable clinical outcomes. However, OLIF-BCAAS exhibits more significant advantages over OLIF-PIFPS by maximizing the benefits of minimally invasive surgery. Moreover, OLIF-BCAAS is associated with lower postoperative back pain and a reduced incidence of postoperative CS.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1571849"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A brief exploration of the physical properties of single living cells under dynamic loading conditions.","authors":"Dasen Xu, Chongyu Zhang, Ruining Peng, Ru Zhang, Haoyu Chen, Yulong Li, Hui Yang","doi":"10.3389/fbioe.2025.1574853","DOIUrl":"10.3389/fbioe.2025.1574853","url":null,"abstract":"<p><strong>Introduction: </strong>Single living cells exhibit both active biological functions and material-like mechanical behaviors. While extensive research has focused on static or quasi-static loading, the purely mechanical properties under high-rate impact remain underexplored. Investigating cell responses to dynamic loading can isolate rapid deformation characteristics, potentially clarifying how life activities modulate mechanical behavior.</p><p><strong>Methods: </strong>We developed a custom dynamic loading system to expose single adherent macrophage cells to transient compression-shear stresses in a controlled fluid environment. A Polymethyl Methacrylate chamber housed the cells, and impact pressures (156.48-3603.85 kPa) were measured in real time using a high-frequency sensor. High-speed imaging (up to 2×10⁵ fps) captured cellular area changes, providing insight into global deformation. In total, 198 valid experiments were performed, and statistical tests confirmed that initial perimeter and area followed normal-like distributions suitable for theoretical analysis.</p><p><strong>Results: </strong>Cells demonstrated a two-stage expansion under shock loading. At lower pressures, cytoplasmic regions rapidly spread into the focal plane, producing significant increases in projected area. As pressure rose further, deformation rate decreased, reflecting the constraining influence of the nucleus. By analyzing the final-to-initial area ratios across various pressures and initial cell sizes, we derived an incomplete state equation akin to Tait-like or Birch-Murnaghan models, indicating an inflection point of maximum deformation rate.</p><p><strong>Discussion: </strong>These findings highlight that fast impact loading effectively minimizes confounding biological processes, revealing intrinsic mechanical responses. The proposed state equation captures cell behavior within milliseconds, offering a path to integrate dynamic results with slower, life-activity-driven adaptations, and laying groundwork for more comprehensive biomechanical models of living cells.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1574853"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Considerations in the selection of patient-reported outcome measures for assessing function related to chronic ankle instability.","authors":"Lijiang Luan, Jeremy Witchalls, Phil Newman, Adrian Pranata, Charlotte Ganderton, Oren Tirosh, Doa El-Ansary, Roger David Adams, Gordon Waddington, Jia Han","doi":"10.3389/fbioe.2025.1602283","DOIUrl":"10.3389/fbioe.2025.1602283","url":null,"abstract":"<p><p>Patient-reported outcome measures (PROMs) are essential tools in evaluating chronic ankle instability (CAI), capturing subjective experiences such as \"giving way\" and instability. However, no standardized guidelines exist for selecting PROMs in CAI, resulting in limited comparability across studies and clinical settings. This paper highlights four key considerations for selecting PROMs in assessing CAI: recalibration in populations including individuals with CAI, identifiability of ankle instability, detectability of CAI characteristics, and cross-cultural adaptability. It emphasizes that CAI-specific PROMs should demonstrate high pertinence, accurately distinguishing CAI from other conditions, and effectively detecting symptom changes over time. Furthermore, widely adopted PROMs may offer greater credibility and applicability. Addressing these considerations is crucial for improving CAI diagnosis, treatment evaluation, and advancing patient-centered care.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1602283"},"PeriodicalIF":4.3,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12185538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144483770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical effects of loading methods on the patellofemoral joint during stair climbing: based on statistical parametric mapping analysis.","authors":"Hongwen Zhang, Xingchen Zhang, Jing Ma, Na Sun, Litai Zhang, Yuan Gao","doi":"10.3389/fbioe.2025.1617823","DOIUrl":"10.3389/fbioe.2025.1617823","url":null,"abstract":"<p><strong>Introduction: </strong>Stair negotiation with external loads imposes substantial demands on the structural and functional integrity of the patellofemoral joint. Current research predominantly focuses on singular loading modalities or level walking conditions, often employing discrete time-point comparisons. This study innovatively employs Statistical Parametric Mapping (SPM) to systematically analyze patellofemoral biomechanical characteristics during stair negotiation with different load-carrying strategies.</p><p><strong>Methods: </strong>Twenty healthy males performed stair negotiation tasks under shoulder-load carriage (SLC) and hand-carry carriage (HCC) conditions (15 kg). Kinematic (200 Hz), kinetic (2000 Hz), and electromyographic (2000 Hz) data were synchronized to compute patellofemoral joint stress(PFJS), center of pressure (COP) trajectories, and muscle co-activation indices across stair phases.</p><p><strong>Results: </strong>HCC generated significantly greater patellofemoral joint stress during most stair phases compared to SLC (<i>P</i> < 0.05), while SLC exhibited transient stress elevation only during initial double-support phase.</p><p><strong>Discussion: </strong>HCC particularly increased joint stress during single-support and second double-support phases, with concomitant increases in COP displacement distances and reduced lower-limb co-cativation indices (CCI) collectively compromising joint stability. Despite transient stress spikes during initial double-support, SLC maintained kinetic chain equilibrium through shorter external moment arms. These findings recommend prioritizing proximal symmetric loading modes complemented by targeted vastus medialis training to enhance patellar stability, thereby reducing both patellofemoral joint stress concentrations and low back pain risks.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1617823"},"PeriodicalIF":4.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing 10-HDA production of <i>Escherichia coli</i> by heterologous expression of MexHID transporter proteins.","authors":"Ziting Xu, Chaofan Du, Sheng Gao, Xinrui Yan, Pan Deng, Yuehan Liu, Junqing Wang, Ruiming Wang, Nan Li","doi":"10.3389/fbioe.2025.1590291","DOIUrl":"10.3389/fbioe.2025.1590291","url":null,"abstract":"<p><p>10-Hydroxy-2-decenoic acid (10-HDA) is a medium-chain α,β-unsaturated carboxylic acid that exists in royal jelly with terminal hydroxylation. It has a broad market value because of its antibacterial, anti-inflammatory, anti-tumor, anti-radiation, and other active functions. The one-step whole-cell catalytic synthesis of 10-HDA by constructing engineered strains has improved the reaction rate to a certain extent compared with the previous two-step method. However, the accumulation of 10-HDA to a certain concentration in engineered <i>Escherichia coli</i> strains will damage the structure and function of cells and even lead to death; this unique antibacterial and antimicrobial activity seriously constrains the production of 10-HDA. In this study, we mined a transporter protein from <i>Pseudomonas aeruginosa</i>, which possesses the ability to efficiently efflux 10-HDA, and constructed a transporter protein overexpression strain by using the multicopy chromosome integration technique, which further improved the efficiency of product efflux, weakened the feedback inhibition of 10-HDA to a certain degree, and increased the substrate conversion rate to 88.6%. 10-HDA was synthesized up to 0.94 g/L by the replenishment flow-addition technique, providing a simple and efficient pathway for the yield breakthrough of 10-HDA biosynthesis.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1590291"},"PeriodicalIF":4.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tooth image segmentation and root canal measurement based on deep learning.","authors":"Ziqing Chen, Qi Liu, Jialei Wang, Nuo Ji, Yuhang Gong, Bo Gao","doi":"10.3389/fbioe.2025.1565403","DOIUrl":"10.3389/fbioe.2025.1565403","url":null,"abstract":"<p><strong>Indroduction: </strong>This study aims to develop a automated method for tooth segmentation and root canal measurement based on cone beam computed tomography (CBCT) images, providing objective, efficient, and accurate measurement results to guide and assist clinicians in root canal diagnosis grading, instrument selection, and preoperative planning.</p><p><strong>Methods: </strong>We utilizes Attention U-Net to recognize tooth descriptors, crops regions of interest (ROIs) based on the center of mass of these descriptors, and applies an integrated deep learning method for segmentation. The segmentation results are mapped back to the original coordinates and position-corrected, followed by automatic measurement and visualization of root canal lengths and angles.</p><p><strong>Results: </strong>Quantitative evaluation demonstrated a segmentation Dice coefficient of 96.33%, Jaccard coefficient of 92.94%, Hausdorff distance of 2.04 mm, and Average surface distance of 0.24 mm - all surpassing existing methods. The relative error of root canal length measurement was 3.42% (less than 5%), and the effect of auto-correction was recognized by clinicians.</p><p><strong>Discussion: </strong>The proposed segmentation method demonstrates favorable performance, with a relatively low relative error between automated and manual measurements, providing valuable reference for clinical applications.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1565403"},"PeriodicalIF":4.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondroitinase ABC in spinal cord injury: advances in delivery strategies and therapeutic synergies.","authors":"Rachel Santana Cunha, Erik Aranha Rossi, Thaís Alves de Santana, Zaquer Suzana Munhoz Costa-Ferro, Bruno Solano de Freitas Souza","doi":"10.3389/fbioe.2025.1604502","DOIUrl":"10.3389/fbioe.2025.1604502","url":null,"abstract":"<p><p>Spinal cord injury (SCI) is a debilitating condition that leads to permanent neurological deficits due to the formation of a glial scar and the accumulation of chondroitin sulfate proteoglycans (CSPGs), which inhibit axonal regeneration. Chondroitinase ABC (ChABC), a bacterial enzyme capable of degrading CSPGs, has emerged as a promising therapeutic strategy for enhancing neural plasticity and functional recovery after SCI. However, clinical translation remains challenging due to the enzyme's thermal instability, short half-life, and limited penetration into the lesion site. This review provides a comprehensive overview of current strategies for ChABC delivery, including direct infusion, nanoparticles, hydrogels, scaffolds, viral vectors, and stem cell-based approaches. We highlight recent technological advances that improve enzyme stability, targeting, and sustained release, as well as combinatorial therapies that enhance tissue regeneration. Although ChABC monotherapy has shown limited efficacy, its association with other regenerative approaches has demonstrated significant potential in preclinical models. Finally, we discuss the translational challenges and future directions required to bring ChABC-based therapies closer to clinical application in SCI patients.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1604502"},"PeriodicalIF":4.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable ion-coordinated double-network conductive hydrogel for spinal cord injury repair.","authors":"Huan Yu, Fan Liu, Yaorui Hu, Weikang Wan, Qing Liu, Shuai Zhou, Luping Zhang, Liming Li, Fei Huang","doi":"10.3389/fbioe.2025.1618680","DOIUrl":"10.3389/fbioe.2025.1618680","url":null,"abstract":"<p><p>The mammalian central nervous system (CNS) demonstrates a severely limited capacity for spontaneous neural regeneration after traumatic spinal cord injury (SCI). Structural repair is also highly constrained due to the inhibitory microenvironment. This inherent limitation persists throughout the recovery phase and often leads to severe motor and sensory dysfunction, profoundly impairing patients' quality of life. Current clinical treatments, including surgical decompression, pharmacological interventions, and rehabilitation therapy, can only partially relieve symptoms. They are not enough to promote neural regeneration and functional recovery. There is an urgent need to develop novel therapeutic approaches to overcome this challenge. This study developed and created an injectable double-network conductive hydrogel, it coordinates iron ions (Fe³⁺) using dynamic Schiff base bonds and metal ion coordination. The conductive hydrogel aids in spinal cord injury repair through various mechanisms, such as reducing glial scar formation, promoting remyelination, and providing neuroprotection. This makes it an injection therapy with promising prospects for clinical translation in the field of nerve regeneration.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1618680"},"PeriodicalIF":4.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic behavior of the nucleus pulposus within the intervertebral disc loading: a systematic review and meta-analysis exploring the concept of dynamic disc model.","authors":"Jean-Philippe Deneuville, Maxime Billot, Alexandra Cervantes, Sylvain Peterlongo, Martin Meyer, Mezika Kolder, Marie Escande, Mathilde Bourgeois, Adrien Pallot, Romain David, Manuel Roulaud, Amine Ounajim, Mark Laslett, Mathieu Sarracanie, Najat Salameh, Arnaud Germaneau, Philippe Rigoard","doi":"10.3389/fbioe.2025.1582438","DOIUrl":"10.3389/fbioe.2025.1582438","url":null,"abstract":"<p><strong>Introduction: </strong>The dynamic disc model (DDM) is a theoretical framework in spine mechanics that theorizes the behavior of the <i>nucleus pulposus</i> within the intervertebral disc under various loads. The model predicts displacement of the <i>nucleus pulposus</i> away from the bending loads, for example backward displacement of the <i>nucleus pulposus</i> with a flexion load. These predictions are regularly used as a theoretical basis for explaining certain disc pathologies, such as disc herniation.</p><p><strong>Methods: </strong>We screened seven databases (CENTRAL, Embase, MEDLINE, CINAHL, ScienceDirect, Google Scholar, and HAL) up to July 2024, identifying studies through a PRISMA-guided approach that detailed the mechanical transformation (displacement and deformation) of the <i>nucleus pulposus</i> under bending load on the intervertebral disc. We conducted a double-blind data extraction and quality assessment of the body of evidence. Finally, we performed a meta-analysis of proportions.</p><p><strong>Results: </strong>From the 9,269 articles screened, 14 studies were included in the systematic review and meta-analysis. Magnetic Resonance Imaging (MRI) was employed in 92.8% of the studies, revealing four strategies for assessing <i>nucleus pulposus</i> transformation. The meta-analysis of asymptomatic subjects' data demonstrated that the <i>nucleus pulposus</i> behavior aligned with dynamic disc model predictions in 85.4% (95% CI = [79.4-91.4]) across spinal regions and bending directions. However, significant heterogeneity and low study quality were noted. Only one study used discography to assess the DDM in a discogenic pain population, identifying discrepancies in <i>nucleus pulposus</i> transformation and contrast agent leakage.</p><p><strong>Conclusion: </strong>Evidence for the dynamic disc model for intact discs is of low strength, whereas very limited evidence challenges the dynamic disc model for fissured discs. New multiparametric MRI studies may help guiding future clinical assessment protocols.</p><p><strong>Systematic review registration: </strong>CRD42022331774.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1582438"},"PeriodicalIF":4.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144474457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}