Frontiers in Bioengineering and Biotechnology最新文献

{"title":"The clinical prospects and challenges of photothermal nanomaterials in myocardium recovery after myocardial infarction.","authors":"Jiali Yang, Jian He, Tian Yue, Haifeng Pei, Shiqiang Xiong, Yue Tang, Jun Hou","doi":"10.3389/fbioe.2024.1491581","DOIUrl":"10.3389/fbioe.2024.1491581","url":null,"abstract":"<p><p>The high morbidity and mortality rates associated with myocardial infarction pose a serious threat to human health. Early diagnosis and appropriate treatment are crucial in saving the lives of patients. In recent years, nanomaterials-based technologies have played a significant role in developing new strategies for cardiac repair, particularly in the use of photothermal nanomaterials, which show great potential in treating myocardial infarction. This review aims to describe the characteristics of photothermal nanomaterials, their effects on cardiomyocyte proliferation and angiogenesis, and the mechanism of cardiac tissue repair. This review serves as a valuable reference for the application of photothermal nanomaterials in the treatment of myocardial infarction, with the ultimate goal of expediting the translation of these treatment strategies into clinical practice.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1491581"},"PeriodicalIF":4.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11558533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An observational study of lower limb muscle imbalance assessment and gait analysis of badminton players.","authors":"Ping Huang, Wenxin Xu, Zeyi Bai, Lin Yu, Qichang Mei, Yaodong Gu","doi":"10.3389/fbioe.2024.1439889","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1439889","url":null,"abstract":"<p><strong>Purpose: </strong>The imbalance of muscle strength indicators has a negative impact on players. Lower limb muscle imbalance can cause gait abnormalities and increase the risk of muscle injury or decreased performance in significantly asymmetrical situations. This study aims to assess the lower limb muscle imbalance and gait feature between the dominant and non-dominant sides of badminton players and the associations between the two variables.</p><p><strong>Methods: </strong>The study included 15 badminton players with years of training experience. Muscle strength and gait parameters were obtained from isokinetic muscle strength testing and plantar pressure analysis systems. The symmetry index was calculated based on formulas such as plantar pressure distribution and percentage of plantar contact area.</p><p><strong>Results: </strong>In the isokinetic muscle strength test, significant differences were found in bilateral knee flexors' average power and total work at 60°/s angular speed. The hamstring to quadriceps ratio (H/Q) range of knee joints of the dominant and non-dominant sides is 0.63-0.74 at low speed, while the H/Q range is 0.81-0.88 at fast speed. The H/Q of bilateral knees increases with increasing angular velocity. As the angular velocity increases, the peak torque to body weight ratio (PT/BW) of the participants' bilateral knee flexors and extensors shows a decreasing trend. The asymmetry score of H/Q at 180°/s angular speed is positively related with step time and stance time. There are varying degrees of differences in gait staging parameters, plantar pressure in each area, plantar contact area, and symmetry index between the dominant and non-dominant sides of badminton players when walking.</p><p><strong>Conclusion: </strong>Badminton players have weaker flexors of the knee joint, imbalanced muscle strength in flexors and extensors, decreased lower limb stability, and a risk of knee joint injury on the non-dominant side. The bending and stretching strength of the knee joint on the dominant side of the players is greater than that on the non-dominant side. The pressure in the first metatarsal region of the dominant side is higher, while that in the midfoot and heel regions is higher on the non-dominant side. badminton players have better forward foot force and heel cushioning ability. Long term badminton sports result in specialized changes in plantar pressure distribution and reduced symmetry.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1439889"},"PeriodicalIF":4.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-assembled lipid-based nanoparticles for chemotherapy against breast cancer.","authors":"Shan Liu","doi":"10.3389/fbioe.2024.1482637","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1482637","url":null,"abstract":"<p><p>Self-assembled lipid-based nanoparticles have been shown to have improved therapeutic efficacy and lower toxic side effects. Breast cancer is a common type of malignant tumor in women. Conventional drugs such as doxorubicin (DOX) have shown low therapeutic efficacy and high drug toxicity in antitumor therapy. This paper surveys research on self-assembled lipid-based nanoparticles by categorizing them under three groups: self-assembled liposomal nanostructures, self-assembled niosomes, and self-assembled lipid-polymer hybrid nanoparticles. Subsequently, the structural features and operating mechanisms of each group are summarized individually along with examples of representative drugs from each group.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1482637"},"PeriodicalIF":4.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11555772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultraviolet laser induced periodic surface structures positively influence osteogenic activity on titanium alloys.","authors":"Luiz Schweitzer, Janosch Schoon, Niklas Bläß, Katrin Huesker, Janine V Neufend, Nikolai Siemens, Sander Bekeschus, Rabea Schlüter, Peter Schneider, Eckart Uhlmann, Georgi Wassilew, Frank Schulze","doi":"10.3389/fbioe.2024.1462232","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1462232","url":null,"abstract":"<p><strong>Background/objective: </strong>Endoprostheses might fail due to complications such as implant loosening or periprosthetic infections. The surface topography of implant materials is known to influence osseointegration and attachment of pathogenic bacteria. Laser-Induced Periodic Surface Structures (LIPSS) can improve the surface topography of orthopedic implant materials. In this preclinical <i>in vitro</i> study, laser pulses with a wavelength in the ultraviolet (UV) spectrum were applied for the generation of LIPSS to positively influence formation of extracellular matrix by primary human Osteoblasts (hOBs) and to reduce microbial biofilm formation <i>in vitro</i>.</p><p><strong>Methods: </strong>Laser machining was employed for generating UV-LIPSS on sample disks made of Ti6Al4V and Ti6Al7Nb alloys. Sample disks with polished surfaces were used as controls. Scanning electron microscopy was used for visualization of surface topography and adherent cells. Metal ion release and cellular metal levels were investigated by inductively coupled plasma mass spectrometry. Cell culture of hOBs on sample disks with and without UV-LIPSS surface treatments was performed. Cells were investigated for their viability, proliferation, osteogenic function and cytokine release. Biofilm formation was facilitated by seeding <i>Staphylococcus aureus</i> on sample disks and quantified by wheat germ agglutinin (WGA) staining.</p><p><strong>Results: </strong>UV-LIPSS modification results in topographies with a periodicity of 223 nm ≤ λ ≤ 278 nm. The release of metal ions was found increased for UV-LIPSS on Ti6Al4V and decreased for UV-LIPSS on Ti6Al7Nb, while cellular metal levels remain unaffected. Cellular adherence was decreased for hOBs on UV-LIPSS Ti6Al4V when compared to controls while proliferation rate was unaffected. Metabolic activity was lower on UV-LIPSS Ti6Al7Nb when compared to the control. Alkaline phosphatase activity was upregulated for hOBs grown on UV-LIPSS on both alloys. Less pro-inflammatory cytokines were released for cells grown on UV-LIPSS Ti6Al7Nb when compared to polished surfaces. WGA signals were significantly lower on UV-LIPSS Ti6Al7Nb indicating reduced formation of a <i>S. aureus</i> biofilm.</p><p><strong>Conclusion: </strong>Our results suggest that UV-LIPSS texturing of Ti6Al7Nb positively influence bone forming function and cytokine secretion profile of hOBs <i>in vitro</i>. In addition, our results indicate diminished biofilm formation on UV-LIPSS treated Ti6Al7Nb surfaces. These effects might prove beneficial in the context of long-term arthroplasty outcomes.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1462232"},"PeriodicalIF":4.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Procollagen-lysine 2-oxoglutarate 5-dioxygenases are responsible for 5R-hydroxylysine modification of therapeutic T-cell bispecific monoclonal antibodies produced by Chinese hamster ovary cells.","authors":"Niels Bauer, Marco Boettger, Styliani Papadaki, Tanja Leitner, Stefan Klostermann, Hubert Kettenberger, Guy Georges, Vincent Larraillet, Dino Gluhacevic von Kruechten, Lars Hillringhaus, Annette Vogt, Simon Ausländer, Oliver Popp","doi":"10.3389/fbioe.2024.1414408","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1414408","url":null,"abstract":"<p><p>We present a detailed mass spectrometric analysis of three 2 + 1 T-cell bispecific monoclonal antibodies (TCB mAbs), where an unexpected +15.9950 Da mass shift in tryptic peptides was observed. This modification was attributed to the occurrence of 5R-hydroxylysine (Hyl) using a hybrid LC-MS/MS molecular characterization and CRISPR/Cas9 gene deletion approach. The modification was found at various sites within TCB mAbs, with a conspicuous hot spot motif mirroring a prior observation where Hyl was mapped to the C_H1-VH Fab domain interface of IgGs. In contrast to the preceding report, our structural modeling analysis on TCB mAbs unveiled substantial differences in the orientation and flexibility of motifs in immediate proximity and across the artificial C_H1-VL cross Fab interface and upstream elbow segment. Utilizing a hybrid database search, RNAseq, and a CRISPR/Cas9 knockout methodology in Chinese hamster ovary (CHO) production cell lines, procollagen-lysine, 2-oxoglutarate 5-dioxygenases (PLODs) were conclusively identified as the catalyzing enzymes accountable for the 5R-Hyl modification in TCB mAbs. To quantitatively inhibit Hyl formation in TCB mAbs, the activity of all three Chinese hamster PLOD isoenzymes needs to be depleted via CRISPR/Cas9 gene knockout. Moreover, our investigation identified cell culture iron availability, process duration, and clonal variability in CHO cells as elements influencing the levels of Hyl formation in TCB mAbs. This research offers a solution for circumventing Hyl formation in therapeutic complex mAb formats, such as TCB mAbs, produced in CHO cell culture processes, thereby addressing potential technical and biological challenges associated with unintended Hyl modification.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1414408"},"PeriodicalIF":4.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diacerein-loaded surface modified iron oxide microparticles (SMIOMPs): an emerging magnetic system for management of osteoarthritis via intra-articular injection.","authors":"Nouran Abdelmageed Ali, Nadia M Morsi, Shaimaa M Badr-Eldin, Rehab N Shamma","doi":"10.3389/fbioe.2024.1439085","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1439085","url":null,"abstract":"<p><strong>Introduction: </strong>Osteoarthritis (OA) is regarded as one of the most prevealent irreversible joint degenerative disorder worldwide. Recently, considerable interest in utilizing intra-articular (IA) injections for managing OA has been raised.</p><p><strong>Methods: </strong>In this study, IA injectable surface modified iron oxide microparticles (SMIOMPs) loaded with Diacerein (DCN) were developed. The effects of formulation parameters on particle size, entrapment efficiency, and zeta potential were explored using factorial design. The optimized formulation was characterized regarding morphology and in vitro release. Differential scanning calorimetry (DSC) and Fourier-transform infrared spectroscopy (FTIR) were done to assess interactions. Further, sterilization and <i>in vivo</i> performance in rats with induced arthritis has been performed for the optimized formulation.</p><p><strong>Results and discussion: </strong>The selected optimized system included 2M FeCL3 and 1% chitosan as a surface modifier achieved high drug entrapment of 85.25% with a PS of 1.54 µm and sustained DCN release. Morphological examination of the optimized formulation revealed spherical particles with chitosan coat. DSC and FTIR results indicated the absence of undesired interactions between DCN and the used components. No significant change in the measured parameters was observed following sterilization using gamma radiation. <i>In vivo</i> assessment revealed superior performance for the optimized formulation in reducing cartilage inflammation and degradation. Plasma levels of tumor necrosis factor α and Interleukin-1 beta, as well as knee diameter, were significantly reduced in the treated groups compared to the untreated ones.</p><p><strong>Conclusion: </strong>Overall, the results suggest that the proposed DCN-loaded SMIOMPs represent a promising advancement in the arena of cartilage regeneration.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1439085"},"PeriodicalIF":4.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing wound healing through innovative technologies: microneedle patches and iontophoresis.","authors":"Yong Xun Jin, Pham Ngoc Chien, Pham Thi Nga, Xin Rui Zhang, Nguyen Ngan Giang, Linh Thi Thuy Le, Thuy-Tien Thi Trinh, Shu Yi Zhou, Sun Young Nam, Chan Yeong Heo","doi":"10.3389/fbioe.2024.1468423","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1468423","url":null,"abstract":"<p><strong>Introduction: </strong>Wound healing is a complex process involving multiple stages, including inflammation, proliferation, and remodeling. Effective wound management strategies are essential for accelerating healing and improving outcomes. The CELLADEEP patch, incorporating iontophoresis therapy and microneedle technology, was evaluated for its potential to enhance the wound healing process.</p><p><strong>Methods: </strong>This study utilized a full-thickness skin defect model in Sprague-Dawley rats, researchers compared wound healing outcomes between rats treated with the CELLADEEP Patch and those left untreated. Various histological staining techniques were employed to examine and assess the wound healing process, such as H&E, MT and immunofluorescence staining. Furthermore, the anti-inflammatory and proliferative capabilities were further investigated using biochemical assays.</p><p><strong>Results: </strong>Macroscopic and microscopic analyses revealed that the CELLADEEP patch significantly accelerated wound closure, reduced wound width, and increased epidermal thickness and collagen deposition compared to an untreated group. The CELLADEEP patch decreased nitric oxide and reactive oxygen species levels, as well as pro-inflammatory cytokines IL-6 and TNF-α, indicating effective modulation of the inflammatory response. Immunofluorescence staining showed reduced markers of macrophage activity (CD68, F4/80, MCP-1) in the patch group, suggesting a controlled inflammation process. Increased levels of vimentin, α-SMA, VEGF, collagen I, and TGF-β1 were observed, indicating enhanced fibroblast activity, angiogenesis, and extracellular matrix production.</p><p><strong>Discussion: </strong>The CELLADEEP patch demonstrated potential in promoting effective wound healing by accelerating wound closure, modulating the inflammatory response, and enhancing tissue proliferation and remodeling. The CELLADEEP patch offers a promising non-invasive treatment option for improving wound healing outcomes.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1468423"},"PeriodicalIF":4.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and metabolic engineering approaches for enhanced biodesulfurization of petroleum fractions.","authors":"Asheemita Bagchi, Preeti Srivastava","doi":"10.3389/fbioe.2024.1482270","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1482270","url":null,"abstract":"<p><p>Sulfur, an abundant component of crude oil, causes severe damage to the environment, poses risks to human health, and poisons the catalysts used in combustion engines. Hydrodesulfurization, the conventionally used method, is not sufficient to remove thiophenes like dibenzothiophene (DBT) and other aromatic heterocyclic compounds. The push for \"ultra-clean\" fuels, with sulfur content less than 15 ppm, drives the need for deep desulfurization. Thus, in conjunction with hydrodesulfurization, efficient and eco-friendly methods of deep desulfurization, like biodesulfurization, are desirable. In biodesulfurization, naturally desulfurizing microorganisms are used, with genetic engineering and biotechnology, to reduce the sulfur content of crude oil to below 15 ppm. In this review, we describe genetic and metabolic engineering approaches reported to date to develop more efficient methods to carry out biodesulfurization, making it a practically applicable reality.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1482270"},"PeriodicalIF":4.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun fibers of zein and pea protein to create high-quality fibrous structures in meat analogs.","authors":"Letícia G da Trindade, Letícia Zanchet, Fabiana Perrechil Bonsanto, Anna Rafaela Cavalcante Braga","doi":"10.3389/fbioe.2024.1483966","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1483966","url":null,"abstract":"<p><strong>Introduction: </strong>The importance of developing plant-based meat similar to animal meat lies in the fact that sensory similarity is a crucial factor in encouraging consumers to adopt this alternative.</p><p><strong>Methodology: </strong>The present study reports the morphology, hydrophilicity, and thermal analysis of different fibers obtained by the electrospinning method. In the first step of this work, zein and zein/poly(ethylene oxide) (PEO) in 80% aqueous ethanol solution with varying concentrations of these polymers were investigated.</p><p><strong>Results and discussion: </strong>It was observed that the diameters of the electrospun fibers are related to the concentration and viscosity of the solutions. Moreover, the addition of small percentages of PEO makes the fibers more hydrophilic and leads to an increase in the polymeric solution viscosity. Because of its low toxicity, PEO is used in various edible products. In the second step of this work, an ideal zein/PEO combination was found to allow the pea protein (PP) to be electrospun. Adding PP to the zein/PEO blend (20:1) leads to a more hydrophilic fiber and improves thermal stability. The results suggest that the zein/PEO and zein/PEO/PP blends can offer an innovative solution to enhance the texture and appearance of plant-based meats. These simulated electrospun fibers can mimic the fibers in animal meat and are a potential alternative to provide a sensory experience as close to animal meat as possible.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1483966"},"PeriodicalIF":4.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11551025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerated innervation of biofabricated skeletal muscle implants containing a neurotrophic factor delivery system.","authors":"Vladimir Mashanov, Erika Billman, Aurelia Poerio, Mary Kaufmann, Dehui Lai, J William Vaughan, Ickhee Kim, Young Min Ju, Anthony Atala, James J Yoo, Ji Hyun Kim","doi":"10.3389/fbioe.2024.1476370","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1476370","url":null,"abstract":"<p><strong>Introduction: </strong>Volumetric muscle loss (VML) is one of the most severe and debilitating conditions in orthopedic and regenerative medicine. Current treatment modalities often fail to restore the normal structure and function of the damaged skeletal muscle. Bioengineered tissue constructs using the patient's own cells have emerged as a promising alternative treatment option, showing positive outcomes in fostering new muscle tissue formation. However, achieving timely and proper innervation of the implanted muscle constructs remains a significant challenge. In this study, we present a clinically relevant strategy aimed at enhancing and sustaining the natural regenerative response of peripheral nerves to accelerate the innervation of biofabricated skeletal muscle implants.</p><p><strong>Methods: </strong>We previously developed a controlled-release neurotrophic factor delivery system using poly (lactic-co-glycolic acid) (PLGA) microspheres encapsulating ciliary neurotrophic factor (CNTF) and glial cell line-derived neurotrophic factor (GDNF). Here, we incorporate this neurotrophic factor delivery system into bioprinted muscle constructs to facilitate innervation <i>in vivo</i>.</p><p><strong>Results: </strong>Our results demonstrate that the neurotrophic factors released from the microspheres provide a chemical cue, significantly enhancing the neurite sprouting and functional innervation of the muscle cells in the biofabricated muscle construct within 12 weeks post-implantation.</p><p><strong>Discussion: </strong>Our approach provides a clinically applicable treatment option for VML through accelerated innervation of biomanufactured muscle implants and subsequent improvements in functionality.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"12 ","pages":"1476370"},"PeriodicalIF":4.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}