Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Challenges of engineering a functional growth plate <i>in vitro</i>.","authors":"Gangyu Zhang, Adrien Moya, Arnaud Scherberich, Ivan Martin","doi":"10.3389/fbioe.2025.1550713","DOIUrl":"10.3389/fbioe.2025.1550713","url":null,"abstract":"<p><p>Several cartilage and bone organoids have been developed <i>in vitro</i> and <i>in vivo</i> using adult mesenchymal stromal/stem cells (MSCs) or pluripotent stem cells (PSCs) to mimic different phases of endochondral ossification (ECO), as one of the main processes driving skeletal development and growth. While cellular and molecular features of growth plate-like structures have been observed through the generation and <i>in vivo</i> implantation of hypertrophic cartilage tissues, no functional analogue or model of the growth plate has yet been engineered. Herein, after a brief introduction about the growth plate architecture and function, we summarize the recent progress in dissecting the biology of the growth plate and indicate the knowledge gaps to better understand the mechanisms of its development and maintenance. We then discuss how this knowledge could be integrated with state-of-art bioengineering approaches to generate a functional <i>in vitro</i> growth plate model.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1550713"},"PeriodicalIF":4.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different exosomes are loaded in hydrogels for the application in the field of tissue repair.","authors":"Yanyan Zhang, Wenjing Yan, Le Wu, Zihao Yu, Ying Quan, Xin Xie","doi":"10.3389/fbioe.2025.1545636","DOIUrl":"10.3389/fbioe.2025.1545636","url":null,"abstract":"<p><p>Exosomes are double-membrane vesicular nanoparticles in the category of extracellular vesicles, ranging in size from 30 to 150 nm, and are released from cells through a specific multi-step exocytosis process. Exosomes have emerged as promising tools for tissue repair due to their ability to transfer bioactive molecules that promote cell proliferation, differentiation, and tissue regeneration. However, the therapeutic application of exosomes is hindered by their rapid clearance from the body and limited retention at the injury site. To overcome these challenges, hydrogels, known for their high biocompatibility and porous structure, have been explored as carriers for exosomes. Hydrogels can provide a controlled release mechanism, prolonging the retention time of exosomes at targeted tissues, thus enhancing their therapeutic efficacy. This review focuses on the combination of different exosomes with hydrogels in the context of tissue repair. We first introduce the sources and functions of exosomes, particularly those from mesenchymal stem cells, and their roles in regenerative medicine. We then examine various types of hydrogels, highlighting their ability to load and release exosomes. Several strategies for encapsulating exosomes in hydrogels are discussed, including the impact of hydrogel composition and structure on exosome delivery efficiency. Finally, we review the applications of exosomes-loaded hydrogels in the repair of different tissues, such as skin, bone, cartilage, and nerve, and explore the challenges and future directions in this field. The combination of exosomes with hydrogels offers significant promise for advancing tissue repair strategies and regenerative therapies.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1545636"},"PeriodicalIF":4.3,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfer learning-enhanced CNN-GRU-attention model for knee joint torque prediction.","authors":"Hao Xie, Yingpeng Wang, Tingting Liu, Songhua Yan, Jizhou Zeng, Kuan Zhang","doi":"10.3389/fbioe.2025.1530950","DOIUrl":"10.3389/fbioe.2025.1530950","url":null,"abstract":"<p><strong>Introduction: </strong>Accurate prediction of joint torque is critical for preventing injury by providing precise insights into the forces acting on joints during activities. Traditional approaches, including inverse dynamics, EMG-driven neuromusculoskeletal (NMS) models, and standard machine learning methods, typically use surface EMG (sEMG) signals and kinematic data. However, these methods often struggle to reveal the complex, non-linear relationship between muscle activation and joint motion, particularly with complex or unfamiliar movements. The generalization of joint torque estimation models across different individuals faces a significant challenge, as feature transferability tends to decline in higher, task-specific layers, reducing model performance.</p><p><strong>Methods: </strong>In this study, we proposed a CNN-GRU-Attention neural network model combining a neuromusculoskeletal (NMS) solver-informed (hybrid-CNN) augmented with transfer learning, designed to predict knee joint torque with higher accuracy. The neural network was trained using EMG signals, joint angles, and muscle forces as inputs to predict knee joint torque in different activities, and the predictive performance of the model was evaluated both within and between subjects. Additionally, we have developed a transfer learning method in the inter-subject model, which improved the accuracy of knee torque prediction by transferring the learning knowledge of previous participants to new participants.</p><p><strong>Results: </strong>Our results showed that the hybrid-CNN model can predict knee joint torque within subjects with a significantly lower error (root mean square error ≤0.16 Nm/kg). A transfer learning technique was adopted in the inter-subject tests to significantly improve the generalizability with a lower error (root mean square error ≤0.14 Nm/kg).</p><p><strong>Conclusion: </strong>The transfer learning-enhanced CNN-GRU-Attention with the NMS model shows great potential in the prediction of knee joint torque.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1530950"},"PeriodicalIF":4.3,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endothelial cell-modified BMSC-GT/PCL nanofiber membrane sheet constructs promote bone tissue regeneration.","authors":"Qian Zhou, Mengnan Wen, Yiwu Zhang, Zhinan Wang, Guangdong Zhou, Xiaoqin Liang","doi":"10.3389/fbioe.2025.1557279","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1557279","url":null,"abstract":"<p><strong>Introduction: </strong>Bone defect repair remains a major challenge in modern medicine. Although bone marrow mesenchymal stem cells (BMSCs) possess multilineage differentiation potential, traditional BMSC constructs are often limited in clinical applications due to insufficient osteogenic differentiation efficiency and inadequate vascularization.</p><p><strong>Methods: </strong>This study developed an innovative bone tissue engineering strategy by combining BMSCs with gelatin/polycaprolactone (GT/PCL) nanofiber membranes to form cell sheets, which were then modified with endothelial cells (ECs) on the surface. The sheets were subsequently rolled into three-dimensional scaffolds to systematically evaluate their osteogenic potential and underlying mechanisms.</p><p><strong>Resuilts: </strong>Results showed that electrospun GT/PCL nanofiber membranes exhibited uniform fiber structure (diameter 200-500 nm), successfully mimicking the microstructure of natural extracellular matrix. <i>In vitro</i> experiments demonstrated that after 14 days of culture, EC modification significantly enhanced the osteogenic differentiation of BMSCs compared to unmodified controls, with approximately 3-fold increase in <i>ALP</i> expression (p < 0.05) and 2.5-fold increase in angiogenic factor <i>VEGF</i> expression (p < 0.01). Subcutaneous implantation in nude mice revealed superior bone formation capability of EC-modified constructs at both 4 and 8 weeks: micro-CT analysis showed bone density reaching 350 mg/cm³, bone surface area approaching 400 mm², and bone volume fraction of approximately 20%, significantly higher than control groups (p < 0.0001). Immunohistochemical evaluation further confirmed more mature trabecular bone structure and richer vascular networks in EC-modified groups.</p><p><strong>Discussion: </strong>Mechanistic studies revealed that EC modification promoted bone regeneration through three key pathways: optimization of local vascular microenvironment for improved nutrient supply, activation of intercellular synergistic signaling pathways, and reconstruction of physiological bone tissue microenvironment. This study not only validates the application value of this composite strategy in bone tissue engineering but also provides important theoretical basis for developing novel bone regeneration solutions.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1557279"},"PeriodicalIF":4.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Batch-fabricated full glassy carbon fibers for real-time tonic and phasic dopamine detection.","authors":"Umisha Siwakoti, May Yoon Pwint, Austin M Broussard, Daniel R Rivera, X Tracy Cui, Elisa Castagnola","doi":"10.3389/fbioe.2025.1543882","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1543882","url":null,"abstract":"<p><p>Dopamine (DA) is a critical neurotransmitter that is key in regulating motor functions, motivation, and reward-related behavior. Measuring both tonic (baseline, steady-state) and phasic (rapid, burst-like) DA release is essential for elucidating the mechanisms underlying neurological disorders, such as schizophrenia and Parkinson's disease, which are associated with dysregulated tonic and phasic DA signaling. Carbon fiber microelectrodes (CFEs) are considered the gold standard for measuring rapid neurotransmitter changes due to their small size (5-10 µm), biocompatibility, flexibility, and excellent electrochemical properties. However, achieving consistent results and large-scale production of CFE arrays through manual fabrication poses significant challenges. We previously developed flexible glassy carbon (GC) microelectrode arrays (MEAs) and GC fiber-like MEAs (GCF MEAs) for neurotransmitter detection and electrophysiology recording. We also demonstrated the feasibility of fabricating GC MEA with both GC electrodes and interconnects made from a single homogeneous material, eliminating the need for metal interconnections and addressing related concerns about electrical and mechanical stability under prolonged electrochemical cycling. Building on our prior experience, we now present a double-etching microfabrication technique for the batch production of 10 μm × 10 µm full GC fibers (fGCFs) and fGCF arrays, composed entirely of homogeneous GC material. This process uses a 2 µm-thick low-stress silicon nitride as the bottom insulator layer for the fGCFs. The effectiveness of the fabrication process was validated through scanning electron microscophy (SEM) and energy dispersive X-ray spectroscopy (EDS) elemental analyses, which confirmed the uniformity of the Si₃N₄ insulation layer and ensured the overall integrity of the fGCFs. Using finite element analysis, we optimized the fGCF form factor to achieve self-penetration up to 3 mm into the mouse striatum without additional support. The electrochemical characterization of fGCFs demonstrated high electrical conductivity and a wide electrochemical window. The ability of fGCFs to detect phasic and tonic DA release was confirmed using fast scan cyclic voltammetry (FSCV) and square wave voltammetry (SWV), respectively, both <i>in vitro</i> and <i>in vivo</i>. With their high sensitivity for phasic and tonic DA detection, combined with a scalable fabrication process and self-supporting insertion capability, fGCFs are promising sensors that offer enhanced practicality for comprehensive DA monitoring.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1543882"},"PeriodicalIF":4.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularization techniques: unveiling the blueprint for tracheal tissue engineering.","authors":"Keisha T Gomes, Palla Ranga Prasad, Jagnoor Singh Sandhu, Ashwini Kumar, Naveena A N Kumar, N B Shridhar, Bharti Bisht, Manash K Paul","doi":"10.3389/fbioe.2025.1518905","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1518905","url":null,"abstract":"<p><p>Certain congenital or acquired diseases and defects such as tracheo-oesophageal fistula, tracheomalacia, tracheal stenosis, airway ischemia, infections, and tumours can cause damage to the trachea. Treatments available do not offer any permanent solutions. Moreover, long-segment defects in the trachea have no available surgical treatments. Tissue engineering has gained popularity in current regenerative medicine as a promising approach to bridge this gap. Among the various tissue engineering techniques, decellularization is a widely used approach that removes the cellular and nuclear contents from the tissue while preserving the native extracellular matrix components. The decellularized scaffolds exhibit significantly lower immunogenicity and retain the essential biomechanical and proangiogenic properties of native tissue, creating a foundation for trachea regeneration. The present review provides an overview of trachea decellularization advancements, exploring how recellularization approaches can be optimized by using various stem cells and tissue-specific cells to restore the scaffold's structure and function. We examine critical factors such as mechanical properties, revascularization, and immunogenicity involved in the transplantation of tissue-engineered grafts.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1518905"},"PeriodicalIF":4.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysregulated biosynthesis and hydrolysis of cyclic-di-adenosine monophosphate impedes sporulation and butanol and acetone production in <i>Clostridium beijerinckii</i> NCIMB 8052.","authors":"Marian M Awaga-Cromwell, Santosh Kumar, Hieu M Truong, Eric Agyeman-Duah, Christopher C Okonkwo, Victor C Ujor","doi":"10.3389/fbioe.2025.1547226","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1547226","url":null,"abstract":"<p><strong>Introduction: </strong>Although solventogenic <i>Clostridium</i> species (SCS) produce butanol, achieving high enough titers to warrant commercialization of biobutanol remains elusive. Thus, deepening our understanding of the intricate cellular wiring of SCS is crucial to unearthing new targets and strategies for engineering novel strains capable of producing and tolerating greater concentrations of butanol.</p><p><strong>Methods: </strong>This study investigated the potential role of cyclic-di-adenosine monophosphate (c-di-AMP) in regulating solvent biosynthesis in <i>C. beijerinckii</i> NCIMB 8052. Genes for c-di-AMP-producing and degrading enzymes [DNA integrity scanning protein A (<i>disA</i>) and phosphodiesterase (<i>pde</i>), respectively] were cloned in this organism and the recombinant strains were characterized relative to the control strain.</p><p><strong>Results: </strong>Plasmid-borne expression of disA in <i>C. beijerinckii</i> led to a 1.83-fold increase in c-di-AMP levels and near complete (∼100%) inhibition of butanol and acetone biosynthesis. Conversely, c-di-AMP concentrations in the pde-expressing strain reduced 7.54-fold relative to the control with 4.20- and 2.3-fold reductions in butanol and acetone concentrations, respectively, when compared to the control strain. Relative to the control and the <i>pde</i>-expressing strains, the <i>disA</i>-expressing strain produced 1.50- and 1.90-fold more ethanol, respectively. Enzyme activity assays show that core solvent biosynthesis enzymes are mostly inhibited <i>in vitro</i> by exogenously supplemented c-di-AMP (50 nM). Both recombinant strains of <i>C. beijerinckii</i> are impaired for sporulation, particularly the <i>disA</i>-expressing strain.</p><p><strong>Discussion: </strong>Collectively, the results show that dysregulated production and hydrolysis of c-di-AMP severely impair butanol and acetone biosynthesis in <i>C. beijerinckii</i>, suggesting broader roles of this second messenger in the regulation of solventogenesis and likely, sporulation in this organism.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1547226"},"PeriodicalIF":4.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable polyester-based hyperbranched nanocarrier-modified with N-acetyl glucosamine for efficient drug delivery to cancer cells through GLUTs.","authors":"Aazam Shaikh, Rajesh Salve, Devyani Sengar, Virendra Gajbhiye","doi":"10.3389/fbioe.2025.1491206","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1491206","url":null,"abstract":"<p><p>Cancer, ranking just below cardiovascular diseases, is a leading cause of mortality worldwide. The key to enhancing survival rates among cancer patients lies in the early detection, removal, and treatment of tumors. However, the broad-spectrum nature of current treatments, including chemotherapy and radiation therapy, results in significant collateral damage to healthy cells and tissues. In this context, hyperbranched polymers present a promising avenue for more targeted therapy. These polymers can be loaded with chemotherapeutic drugs and modified with specific ligands to selectively target cancer cells via glucose transporters, which are overexpressed in many cancer types. To enhance the delivery of drugs to cancer cells, we have engineered an N-acetyl glucosamine conjugated version of this polymer. The characterization of these nanocarriers was evaluated using various techniques, including ¹H NMR, dynamic light scattering, and FTIR spectroscopy. Additionally, confocal microscopy was utilized to compare the accumulation of doxorubicin in cancer cells using both the N-acetyl glucosamine-conjugated and unmodified versions of H40 Boltorn™. Our observations indicated a superior accumulation of doxorubicin in cells treated with the modified H40 polymer. Further evaluation of the drug-loaded nanocarriers was conducted on MDA-MB-231 and 4T1 breast cancer cell lines, focusing on their cytotoxic effects. This suggests that the targeted delivery of anticancer drugs using the modified H40 Boltorn™ nanocarriers significantly enhances the ability to kill breast cancer cells, offering a more efficient and selective approach to chemotherapy that minimizes impact on healthy tissues and cells.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1491206"},"PeriodicalIF":4.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mathematical modeling of a MoSe₂-based SPR biosensor for detecting SARS-CoV-2 at nM concentrations.","authors":"Talia Tene, Nataly Bonilla García, Jessica Alexandra Marcatoma Tixi, Martha Ximena Dávalos Villegas, Cristian Vacacela Gomez, Stefano Bellucci","doi":"10.3389/fbioe.2025.1547248","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1547248","url":null,"abstract":"<p><p>The rapid and accurate detection of SARS-CoV-2 remains a critical challenge in biosensing technology, necessitating the development of highly sensitive and selective platforms. In this study, we present a mathematical modeling approach to optimize a MoSe₂-based Surface Plasmon Resonance (SPR) biosensor for detecting the novel coronavirus at nM scale. Using the Transfer Matrix Method (TMM), we systematically optimize the biosensor's structural parameters, including silver (Ag), silicon nitride (Si₃N₄), molybdenum diselenide (MoSe₂), and thiol-tethered single-stranded DNA (ssDNA) layers, to enhance sensitivity, detection accuracy, and optical performance. The results indicate that an optimized 45 nm Ag layer, 10 nm Si₃N₄ layer, and monolayer MoSe₂ configuration achieves a resonance shift (Δθ) of 0.3° at 100 nM, with a sensitivity of 197.70°/RIU and a detection accuracy of 5.24 × 10⁻². Additionally, the incorporation of a 10 nm ssDNA functionalization layer significantly enhances molecular recognition, lowering the limit of detection (LoD) to 2.53 × 10⁻⁵ and improving overall biosensing efficiency. Sys₅ (MoSe₂ + ssDNA) outperforms Sys₄ (MoSe₂ without ssDNA) in terms of specificity and reliability, making it more suitable for practical applications. These findings establish the MoSe₂-based SPR biosensor as a highly promising candidate for SARS-CoV-2 detection, offering a balance between high sensitivity, optical stability, and molecular selectivity, crucial for effective viral diagnostics.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1547248"},"PeriodicalIF":4.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyanobacterial type I CRISPR-Cas systems: distribution, mechanisms, and genome editing applications.","authors":"Yongjiu Zhang, Shuxiao Yang, Xianliang Zheng, Xiaoming Tan","doi":"10.3389/fbioe.2025.1552030","DOIUrl":"10.3389/fbioe.2025.1552030","url":null,"abstract":"<p><p>Cyanobacteria, renowned for their photosynthetic capabilities, serve as efficient microbial chassis capable of converting carbon dioxide into a spectrum of bio-chemicals. However, conventional genetic manipulation strategies have proven incompatible with the precise and systematic modifications required in the field of cyanobacterial synthetic biology. Here, we present an in-depth analysis of endogenous CRISPR-Cas systems within cyanobacterial genomes, with a particular focus on the Type I systems, which are the most widely distributed. We provide a comprehensive summary of the reported DNA defense mechanisms mediated by cyanobacterial Type I CRISPR-Cas systems and their current applications in genome editing. Furthermore, we offer insights into the future applications of these systems in the context of cyanobacterial genome editing, underscoring their potential to revolutionize synthetic biology approaches.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1552030"},"PeriodicalIF":4.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11903412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}