Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Bioimpedance measurement: a non-invasive diagnosis of limb compartment syndrome.","authors":"Martin Novak, Vladimir Jecminek, Leopold Pleva, Marek Penhaker, Martin Schmidt, Tomas Mimra, Jan Kubicek, Martin Augustynek","doi":"10.3389/fbioe.2024.1433284","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1433284","url":null,"abstract":"<p><strong>Introduction: </strong>The methods for diagnosing compartment syndrome non-invasively remain under debate. Bioimpedance measurements offer a promising avenue in clinical practice, detecting subtle changes in organ impedance due to volume shifts. This study explores bioimpedance measurement as a novel, painless method for diagnosing compartment syndrome, potentially enabling continuous monitoring.</p><p><strong>Objective: </strong>This work aims to develop a prototype device for non-invasive diagnosis of compartment syndrome based on bioimpedance changes and assess initial results through <i>in vitro</i> experiments on inanimate biological material. We assume a change in the bioimpedance value after the application of physiological solution.</p><p><strong>Materials and methods: </strong>Between 2018 and 2022, a prototype device for diagnosing limb compartment syndrome was collaboratively developed with the Department of Cybernetics and Biomedical Engineering at the Technical University of Ostrava, Czech Republic. This device operates by comparing bioimpedance between two compartments, one of which is pathologically affected (experiencing compartment syndrome). The Bioimpedance Analyzer for Compartment Syndrome (BACS) has been utilized to conduct measurements on inanimate biological material in laboratory settings. Two samples of duck and chicken tissue, as well as piglets, were employed for these experiments. According to the size of sample was compartment syndrome simulated by injecting 20-120 mL saline into one limb (breast) while leaving the other as a control. Invasive intramuscular pressure measurements were conducted post-saline injection using a conventional device (Stryker). Changes in bioimpedance were evaluated following saline application.</p><p><strong>Results: </strong>The non-invasive bioimpedance measurement instrument has been developed. It meets the safety requirements of European standard EN 60601-1. Measurement of accuracy showed minimal deviation for both channels (1.08% for the left channel and 1.84% for the right channel) when measuring on resistors. Ten measurements were conducted using the BACS prototype - two on chicken legs, two on duck breasts, two on duck legs, and four on piglets. Compartment syndrome simulation was achieved for all 10 measurements (IMP variance 31-45 mmHg). Following saline application, a notable decrease in bioimpedance was observed in the compartment simulating compartment syndrome (decrease by 12-78 Ω).</p><p><strong>Conclusion: </strong>Non-invasive methods could revolutionize limb compartment syndrome diagnosis, offering advantages such as non-invasiveness and continuous monitoring of compartment swelling.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-domain prediction approach of human lower limb voluntary movement intention for exoskeleton robot based on EEG signals.","authors":"Runlin Dong, Xiaodong Zhang, Hanzhe Li, Zhufeng Lu, Cunxin Li, Aibin Zhu","doi":"10.3389/fbioe.2024.1448903","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1448903","url":null,"abstract":"<p><strong>Background and objective: </strong>Exoskeleton robot control should ideally be based on human voluntary movement intention. The readiness potential (RP) component of the motion-related cortical potential is observed before movement in the electroencephalogram and can be used for intention prediction. However, its single-trial features are weak and highly variable, and existing methods cannot achieve high cross-temporal and cross-subject accuracies in practical online applications. Therefore, this work aimed to combine a deep convolutional neural network (CNN) framework with a transfer learning (TL) strategy to predict the lower limb voluntary movement intention, thereby improving the accuracy while enhancing the model generalization capability; this would also provide sufficient processing time for the response of the exoskeleton robotic system and help realize robot control based on the intention of the human body.</p><p><strong>Methods: </strong>The signal characteristics of the RP for lower limb movement were analyzed, and a parameter TL strategy based on CNN was proposed to predict the intention of voluntary lower limb movements. We recruited 10 subjects for offline and online experiments. Multivariate empirical-mode decomposition was used to remove the artifacts, and the moment of onset of voluntary movement was labeled using lower limb electromyography signals during network training.</p><p><strong>Results: </strong>The RP features can be observed from multiple data overlays before the onset of voluntary lower limb movements, and these features have long latency periods. The offline experimental results showed that the average movement intention prediction accuracy was 95.23% ± 1.25% for the right leg and 91.21% ± 1.48% for the left leg, which showed good cross-temporal and cross-subject generalization while greatly reducing the training time. Online movement intention prediction can predict results about 483.9 ± 11.9 ms before movement onset with an average accuracy of 82.75%.</p><p><strong>Conclusion: </strong>The proposed method has a higher prediction accuracy with a lower training time, has good generalization performance for cross-temporal and cross-subject aspects, and is well-prioritized in terms of the temporal responses; these features are expected to lay the foundation for further investigations on exoskeleton robot control.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lumbar osteopathic manipulative treatment can improve KOA symptoms: short-term efficacy observation and mechanism analysis.","authors":"Peiyu Du, Xi Li, Shilin Yin, Wenyi Li, Xilong Sun, Zekun Zhang, Jianyong Zhao, Gao Shijun, Shuangqing Du, Di Zhang","doi":"10.3389/fbioe.2024.1431527","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1431527","url":null,"abstract":"<p><strong>Background: </strong>Manipulative treatment can effectively improve knee pain and function, but no previous studies have shown that lumbar osteopathic manipulative treatment can improve knee symptoms. To explore the influence of lumbar manipulation on KOA and analyze its principlerelationship between coronal position of lumbar spine and KOA.</p><p><strong>Methods: </strong>Patients were divided into OMT group and DT group according to treatment. WOMAC scores were compared between the two groups, and X-ray examinations before and after treatment were performed in OMT group to analyze the imaging changes.</p><p><strong>Results: </strong>Both OMT group and DT group showed significant improvement in WOMAC score after treatment, and the improvement in OMT group was better than that in DT group. After OMT treatment, cTMI(<i>P</i> = 0.034), mL-SOD (<i>P</i> < 0.001), mΔL-KOD (<i>P</i> = 0.001), LL (<i>P</i> = 0.036), and FTA(<i>P</i> = 0.026) were significantly changed.</p><p><strong>Conclusion: </strong>Compared with drug therapy, lumbar manipulation can better improve WOMAC scores in KOA patients. It relives symptoms by loosening muscles and correcting small joint disorders to improve local knee alignment.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving regioselectivity of phenylalanine aminomutase from <i>Taxus chinensis</i> by semi-rational mutagenesis for the biocatalytic amination of cinnamates.","authors":"Tao Tang, Miao Wang, Yunyun Zhang, Yijun Chen","doi":"10.3389/fbioe.2024.1417962","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1417962","url":null,"abstract":"<p><p>The occupancy of the binding pocket by the substrate ultimately determines the outcome of enzyme catalysis. Previous engineering and substrate scope of phenylalanine aminomutase from <i>Taxus chinensis</i> (TcPAM) has generated valuable knowledge about the regioselectivity with biocatalytic potentials for the preparation of α- and β-phenylalanine and their derivatives. However, the significantly different regioselectivity during the amination of cinnamates by TcPAM is not fully understood. In this study, we take a reconstruction approach to change the whole binding pocket of TcPAM for probing the factors affecting the regioselectivity, resulting in variant C107S/Q319M/I431V reaching a 25.5-fold enhancement of the β/α product ratio toward <i>trans</i>-cinnamate acid. Furthermore, when substituted cinnamates were used as substrates, the regioselectivity was strongly correlated with various changes in the binding pocket, and value-added 2-Cl-α-Phe (100% α-selectivity) and 4-CH₃-β-Phe (98% β-selectivity) were individually verified by the mutants L104A and Q319M at a preparative scale, exemplifying the application feasibility of our engineering strategy. The present study uncovered the cooperative connection between aromatic binding and carboxylate binding to affect the regioselectivity, which provides new insights into the determinants of the regioselectivity possessed by TcPAM and paves the way for its biocatalytic applications on phenylalanine derivatives.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification, structural characterization, and bioactive properties of exopolysaccharides from <i>Saccharomyces cerevisiae</i> HD-01.","authors":"Ruoxi Yang, Lina Liu, Dongni Gao, Dan Zhao","doi":"10.3389/fbioe.2024.1455708","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1455708","url":null,"abstract":"<p><p>Exopolysaccharides (EPSs), which show excellent biological activities, like anti-tumor, immune regulation, and anti-oxidation activities, have gained widespread attention. In this study, an EPS-producing <i>Saccharomyces cerevisiae</i> HD-01 was identified based on 18S rDNA sequence analysis and an API 20C test. The purified HD-01 EPS was obtained by gel filtration chromatography. High-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) revealed that it was a heteropolysaccharide composed of <i>α</i>-1 (38.3%), <i>α</i>-1, 2 (17.5%), <i>α</i>-1, 6 (14.8%)-linked mannose and <i>α</i>-1, 2, 3, 6 (24.3%), <i>α</i>-1 (3.3%), <i>β</i>-1, 4 (1.8%)-linked glucose. Chemical composition and elemental analysis indicated the existence of sulfation modifications. A scanning electron microscope (SEM) and an atomic force microscope (AFM) revealed that it exhibited a flaky structure with thorn-like protrusions on the three-dimensional surface. X-ray diffraction (XRD) revealed that it was an amorphous non-crystalline substance. HD-01 EPS had great thermostability; probiotic properties; strong antioxidant properties to DPPH, ABTS, and hydroxyl; and good reducing power. The MTT, NO, and neutral red assays demonstrated that it had a great immunomodulatory effect on macrophages RAW264.7. All results suggested that the HD-01 EPS had the potential to be applied in the food and pharmaceutical fields.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomedicines in diagnosis and treatment of prostate cancers: an updated review","authors":"Jiajia Wang, Xuan Zhang, Jiazhen Xing, Lijian Gao, Hua Lu","doi":"10.3389/fbioe.2024.1444201","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1444201","url":null,"abstract":"Prostate cancer (PC) is the third most common male cancer in the world, which occurs due to various mutations leading to the loss of chromatin structure. There are multiple treatments for this type of cancer, of which chemotherapy is one of the most important. Sometimes, a combination of different treatments, such as chemotherapy, radiotherapy, and surgery, are used to prevent tumor recurrence. Among other treatments, androgen deprivation therapy (ADT) can be mentioned, which has had promising results. One of the drawbacks of chemotherapy and ADT treatments is that they are not targeted to the tumor tissue. For this reason, their use can cause extensive side effects. Treatments based on nanomaterials, known as nanomedicine, have attracted much attention today. Nanoparticles (NPs) are one of the main branches of nanomedicine, and they can be made of different materials such as polymer, metal, and carbon, each of which has distinct characteristics. In addition to NPs, nanovesicles (NVs) also have therapeutic applications in PC. In treating PC, synthetic NVs (liposomes, micelles, and nanobubbles) or produced from cells (exosomes) can be used. In addition to the role that NPs and NVs have in treating PC, due to being targeted, they can be used to diagnose PC and check the treatment process. Knowing the characteristics of nanomedicine-based treatments can help design new treatments and improve researchers’ understanding of tumor biology and its rapid diagnosis. In this study, we will discuss conventional and nanomedicine-based treatments. The results of these studies show that the use of NPs and NVs in combination with conventional treatments has higher efficacy in tumor treatment than the individual use of each of them.","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical study on the cerebral blood flow regulation in the circle of Willis with the vascular absence and internal carotid artery stenosis.","authors":"Hong Lv, Kailei Fu, Wei Liu, Zhiyi He, Zhiqing Li","doi":"10.3389/fbioe.2024.1467257","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1467257","url":null,"abstract":"<p><strong>Objectives: </strong>This study explores how vascular stenosis and absence affect the regulation of cerebral blood flow in the Circle of Willis (CoW) and the hemodynamic changes downstream of the stenosis.</p><p><strong>Materials and methods: </strong>Forty idealized CoW models were simulated to analyze the impact of vascular absence and internal carotid artery (ICA) stenosis on hemodynamics. Inlet conditions were set using a physiological pressure waveform, and outflow boundaries were modeled using three-element Windkessel models.</p><p><strong>Results: </strong>The absence of vessels such as RP1, LP1, RA1, or LA1 had a comparable effect on total blood flow to a 40% stenosis of the left internal carotid artery (LICA) across the entire CoW. Specifically, when LP1 and RA1 were absent with a 50% LICA stenosis, the total blood flow closely resembled that of a complete CoW with 75% LICA stenosis. In cases of proximal ICA stenosis, downstream regions showed elevated oscillatory shear index (OSI >0.2) and reduced time-averaged wall shear stress (TAWSS <1 Pa). With increasing stenosis severity, areas of high OSI shifted, and regions of low TAWSS expanded notably. At 75% stenosis, the area with TAWSS <1 Pa downstream significantly increased. Until complete occlusion, the area of low TAWSS and high OSI were maximized.</p><p><strong>Conclusion: </strong>This study underscores how anatomical variations in the CoW, combined with ICA stenosis, impact both total cerebral blood flow and its distribution among different outlets. Moreover, it highlights the potential for increased atherosclerosis development in affected areas. Particularly notable is the finding the absence of LP1 and RA1 vessels alongside 50% LICA stenosis results in blood flow patterns similar to those seen with 75% LICA stenosis in a complete CoW, emphasizing clinical implications for the patient. Hemodynamic changes, including TAWSS and OSI, are most pronounced downstream of the stenosis especially when the stenosis rate exceeds 75%.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11374663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: Targeted delivery of 5-fluorouracil, miR-532-3p, and si-KRAS to the colorectal tumor using layer-by-layer liposomes.","authors":"","doi":"10.3389/fbioe.2024.1478182","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1478182","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.3389/fbioe.2022.1013541.].</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing tracheal reconstruction: innovations in vascularized composite allograft transplantation.","authors":"Yiyuan Zhang, Shixiong Wei, Mingqian Li, Guoyue Lv","doi":"10.3389/fbioe.2024.1452780","DOIUrl":"10.3389/fbioe.2024.1452780","url":null,"abstract":"<p><p>Tracheal defects, particularly those extending over long segments, present substantial challenges in reconstructive surgery due to complications in vascularization and integration with host tissues. Traditional methods, such as extended tracheostomies and alloplastic stents, often result in significant morbidity due to mucus plugging and mechanical erosion. Recent advances in vascularized composite allograft (VCA) transplantation have opened new avenues for effective tracheal reconstruction. This article reviews the evolution of tracheal reconstruction techniques, focusing on the shift from non-vascularized approaches to innovative revascularization methods that enhance graft integration and functionality. Key advancements include indirect revascularization techniques and the integration of regenerative medicine, which have shown promise in overcoming historical barriers to successful tracheal transplantation. Clinical case studies are presented to illustrate the complexities and outcomes of recent tracheal transplantation procedures, highlighting the potential for long-term success through the integration of advanced vascular engineering and immune modulation strategies. Furthermore, the role of chimerism in reducing graft rejection and the implications for future tracheal transplantation and tissue engineering efforts are discussed. This review underscores the transformative potential of VCA in tracheal reconstruction, paving the way for more reliable and effective treatments for extensive tracheal defects.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the state of the art: a systematic review and meta-analysis of paper-based microfluidic devices.","authors":"Rodrigo García-Azuma, Karen Werner, Cristina Revilla-Monsalve, Oscar Trinidad, Nelly F Altamirano-Bustamante, Myriam M Altamirano-Bustamante","doi":"10.3389/fbioe.2024.1421831","DOIUrl":"10.3389/fbioe.2024.1421831","url":null,"abstract":"<p><strong>Introduction: </strong>This systematic review and meta-analysis present a comprehensive evaluation of paper-based microfluidic devices, focusing on their applications in immunoassays. These devices are emerging as innovative solutions to democratize access to diagnostic technologies, especially in resource-limited settings. Our review consolidates findings from diverse studies to outline advancements in paper-based microfluidic technology, including design intricacies and operational efficacy. Key advantages such as low cost, portability, and ease of use are highlighted.</p><p><strong>Materials and methods: </strong>The review categorizes literature based on the design and operational nuances of these diagnostic tools, exploring various methodologies, fabrication techniques, detection methods, and applications, particularly in protein science. The meta-analysis extends to the diverse applications of these technologies, providing a framework for classifying and stratifying their uses in diagnostics.</p><p><strong>Results and discussion: </strong>Notable findings include a critical analysis of performance metrics, such as sensitivity and specificity. The review addresses challenges, including the need for further validation and optimization for broader clinical applications. A critical discussion on the validation processes, including cross-validation and rigorous control testing, is provided to ensure the robustness of microfluidic devices. This study offers novel insights into the computational strategies underpinning these technologies and serves as a comprehensive roadmap for future research, potentially broadening the impact across the protein science universe.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}