Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Semaphorin 3A promotes the long-term persistence of human SVF-derived microvascular networks in engineered grafts.","authors":"Juan M Schwager, Nunzia Di Maggio, Andrea Grosso, Abeelan Rasadurai, Nadja Minder, Jeffrey A Hubbell, Elisabeth A Kappos, Dirk J Schaefer, Priscilla S Briquez, Andrea Banfi, Maximilian G Burger","doi":"10.3389/fbioe.2024.1396450","DOIUrl":"10.3389/fbioe.2024.1396450","url":null,"abstract":"<p><strong>Introduction: </strong>The stromal vascular fraction (SVF) of human adipose tissue is an attractive cell source for engineering grafts with intrinsic vascularization potential, as it is rich in vasculogenic progenitors. However, in order to maintain their functional perfusion it is important to promote the <i>in vivo</i> stabilization of newly assembled microvascular networks. We previously found that Semaphorin 3A (Sema3A) promotes the rapid stabilization of new blood vessels induced by VEGF overexpression in skeletal muscle. Here we investigated whether Sema3A could promote the assembly, connection to circulation and persistence of human SVF-derived microvascular networks in engineered grafts.</p><p><strong>Methods: </strong>Recombinant Sema3A was engineered with a transglutaminase substrate sequence (TG-Sema3A) to allow cross-linking into fibrin hydrogels. Grafts were prepared with freshly isolated human SVF cells in fibrin hydrogels decorated with 0, 0.1 or 100 μg/ml TG-Sema3A and implanted subcutaneously in immune-deficient mice.</p><p><strong>Results: </strong>After 1 week in vivo, the assembly of human-derived networks was similar in all conditions. The outer part of the grafts was populated by blood vessels of both human and mouse origin, which formed abundant hybrid structures within a common basal lamina. About 90% of human-derived blood vessels were functionally connected to the host circulation in all conditions. However, in the control samples human vessels were unstable. In fact, they significantly regressed by 6 weeks and could no longer be found by 12 weeks. In contrast, a low Sema3A dose (0.1 μg/ml) promoted further human vascular expansion by about 2-fold at 6 weeks and protected them from regression until 12 weeks. From a mechanistic point of view, the stabilization of SVF-derived vessels by 0.1 μg/ml of Sema3A correlated with the recruitment of a specific population of monocytes expressing its receptor Neuropilin-1.</p><p><strong>Discussion: </strong>In conclusion, Sema3A is a potent stimulator of <i>in vivo</i> long-term persistence of microvascular networks derived from human SVF. Therefore, decoration of matrices with Sema3a can be envisioned to promote the functional support of tissue engineered grafts.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiofrequency ablation with sine and square electrical waveforms to enhance ablation range.","authors":"Dong-Sung Won, Jinsu An, Ji Won Kim, Yubeen Park, Sang Soo Lee, Hyung-Sik Kim, Jung-Hoon Park","doi":"10.3389/fbioe.2024.1450331","DOIUrl":"10.3389/fbioe.2024.1450331","url":null,"abstract":"<p><p>Radiofrequency ablation (RFA) is a local treatment modality for primary liver cancers. Although various input parameters of the RF generator have been adjusted to improve the ablation ranges, the limited ablation ranges remain an obstacle to RFA. This study aimed to compare the ablation ranges and efficacy of sine and square electrical waveforms in a mouse tumor model. An RF generator with an adjustable electrical waveform was developed, and its ablation range in the porcine liver was compared. For all RF parameters, the square electrical waveform ablation range was greater than that of the sine electrical waveform (all <i>p</i> < 0.001) in the porcine liver. The 45 BALB/c nude mice were used to evaluate the efficacy of the two electrical waveforms after the RFA. The mean tumor volume in the square group was significantly lower than that in the sine group (<i>p</i> < 0.001), indicating a higher survival rate (60%). The cellular coagulative necrosis, inflammatory cell infiltration, heat shock proteins, cellular necrosis, and tumor necrosis were significantly greater in square electrical waveform than in sine electrical waveform (all; <i>p</i> < 0.05). RFA with square electrical waveforms has therapeutic potential for tumor management with an enhanced ablation range.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding public perspectives on genetically engineered Brinjal and the adoption of modern biotechnology in Bangladesh.","authors":"Sium Ahmed, Abdullah Mohammad Shohael, Tanvir Ahamed, Razu Ahmed, Shawon Ahmed, H M Shahid Hassan","doi":"10.3389/fbioe.2024.1471201","DOIUrl":"10.3389/fbioe.2024.1471201","url":null,"abstract":"<p><p>The agricultural sector in Bangladesh is currently facing numerous challenges. The country is currently endeavoring to adopt modern biotechnological tools, such as genetic engineering, to modify crops with the aim of ensuring food security. Notably, Bt Brinjal represents a significant milestone as the first genetically engineered (GE) food crop commercially cultivated in South Asia. Public perception and awareness are crucial steps forward for accepting and commercializing GE crops within society. The study discussed here aims to assess public perception and awareness regarding modern biotechnology and GE crops, focusing mainly on Bt Brinjal in Bangladesh. A random survey considered demographic factors such as age, gender, hometown, educational qualification, and occupation to explore the public attitudes towards Bt Brinjal and modern biotechnology. Approximately one-third of those surveyed considered Bt Brinjal safe for consumption, and a third expressed a willingness to buy Bt Brinjal, while nearly two-thirds believed it would gain popularity in the market alongside other crops. Most respondents recognized the necessity of utilizing modern biotechnology for crop improvement beyond Bt Brinjal, and respondents with science backgrounds displayed higher awareness and a more positive attitude than those with limited education or non-science backgrounds. This study explores the public perceptions of Bt Brinjal and the adoption of modern biotechnology in Bangladesh by examining factors such as knowledge dissemination, acceptance levels, and concerns related to GE crops, and offers a meaningful perspective that can shape decision-making processes to promote agricultural sustainability and achieve relevant sustainable development goals in Bangladesh.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered activation patterns of the sensory-motor cortex in patients with knee osteoarthritis during knee isokinetic movement at different speeds.","authors":"Kun Yang, Yuwu Ding, Lixi Chu, Changfeng Cheng, Xiaoming Yu, Haichen Xu, Ying Tao, Tiantian Liu, Lei Yin, Xubo Wu, Bingli Liu, Liming Jiang","doi":"10.3389/fbioe.2024.1444731","DOIUrl":"10.3389/fbioe.2024.1444731","url":null,"abstract":"<p><strong>Background: </strong>Abnormal brain activation patterns in patients with knee osteoarthritis (KOA) at rest have been revealed, but it is unclear how brain activation patterns change during movement. This study aimed to investigate the alterations in brain activation patterns in KOA patients during knee isokinetic movement, and the correlation between cortical activity changes and pain severity and dysfunction.</p><p><strong>Methods: </strong>Eighteen patients with KOA and 18 healthy controls (HC) were recruited, and to performed the knee isokinetic test with three speeds. Functional near-infrared spectroscopy (fNIRS) was used to detect the cerebral cortex hemodynamics changes of primary somatosensory (S1), primary motor (M1) and somatosensory association cortex (SAC) in the region of interest (ROI) during movement. Then, we evaluated potential correlations between M1, S1 and SAC values and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and visual analog scale (VAS) scores.</p><p><strong>Results: </strong>The results showed that peak torque of knee extension in KOA patients was significantly smaller than that in HC. For HC, unilateral knee movement activated bilateral ROIs. The contralateral activation was dominant, showing the phenomenon of high contralateral activation. For KOA patients, there were no statistical difference in the activation level between the left and right of the cerebral cortex, with both sides showing lower activation levels compared to HC. Further analysis found that the contralateral M1, S1, and SAC of the affected knee in KOA patients were significantly lower than those in HC, while no difference was found on the ipsilateral side. Moreover, during isokinetic movement at 180°/s, VAS score in KOA patients was negatively correlated with the activation level of the contralateral S1 and M1 values, and WOMAC was negatively correlated with the activation level of the contralateral M1 value.</p><p><strong>Conclusion: </strong>Contralateral activation of the sensorimotor cortex exists during unilateral knee movement, but in KOA patients, this contralateral cortical activation is suppressed. Furthermore, the clinical pain and dysfunction in KOA patients are associated with activation levels of specific brain regions. These findings can provide a better understanding of KOA brain science and are expected to contribute to the development of central intervention for the disease.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction: Multicomponent siRNA/miRNA-loaded modified mesoporous silica nanoparticles targeted bladder cancer for a highly effective combination therapy.","authors":"","doi":"10.3389/fbioe.2024.1478191","DOIUrl":"https://doi.org/10.3389/fbioe.2024.1478191","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.3389/fbioe.2022.949704.].</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun bioresorbable polymer membranes for coronary artery stents.","authors":"Maria A Rezvova, Evgeny A Ovcharenko, Kirill Yu Klyshnikov, Tatiana V Glushkova, Alexander E Kostyunin, Daria K Shishkova, Vera G Matveeva, Elena A Velikanova, Amin R Shabaev, Yulia A Kudryavtseva","doi":"10.3389/fbioe.2024.1440181","DOIUrl":"10.3389/fbioe.2024.1440181","url":null,"abstract":"<p><p>Percutaneous coronary intervention, a common treatment for atherosclerotic coronary artery lesions, occasionally results in perforations associated with increased mortality rates. Stents coated with a bioresorbable polymer membrane may offer an effective solution for sealing coronary artery perforations. Additionally, such coatings could be effective in mitigating neointimal hyperplasia within the vascular lumen and correcting symptomatic aneurysms. This study examines polymer membranes fabricated by electrospinning of polycaprolactone, polydioxanone, polylactide-co-caprolactone, and polylactide-co-glycolide. In uniaxial tensile tests, all the materials appear to surpass theoretically derived elongation thresholds necessary for stent deployment, albeit polydioxanone membranes are found to disintegrate during the experimental balloon expansion. As revealed by <i>in vitro</i> hemocompatibility testing, polylactide-co-caprolactone membranes exhibit higher thrombogenicity compared to other evaluated polymers, while polylactide-co-glycolide samples fail within the first day post-implantation into the abdominal aorta in rats. The PCL membrane exhibited significant water leakage in the permeability test. Comprehensive evaluation of mechanical testing, bio- and hemocompatibility, as well as biodegradation dynamics shows the advantage of membranes based on and the mixture of polylactide-co-caprolactone and polydioxanone over other polymer groups. These findings lay a foundational framework for conducting preclinical studies on stent configurations in large laboratory animals, emphasizing that further investigations under conditions closely mimicking clinical use are imperative for making definitive conclusions.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of controlled release of HGF on extracellular vesicle secretion by urine-derived stem cells.","authors":"Abdelrahman Alwan, Fatma Khalil, Joshua Bowlby, Gabrielle Peko, Exel Valle Estrada, Sangeeta Singh, Gagan Deep, Yuanyuan Zhang, Alan C Farney, Emmanuel C Opara","doi":"10.3389/fbioe.2024.1436296","DOIUrl":"10.3389/fbioe.2024.1436296","url":null,"abstract":"<p><strong>Introduction: </strong>The hepatic growth factor (HGF) stimulates DNA synthesis and cell proliferation and plays a role in tissue protection and regeneration. In this study, we have examined the effect of incubation of HGF with urine-derived stem cells (USCs) on the secretion of small extracellular vesicles (sEV) by the cells.</p><p><strong>Materials and methods: </strong>HGF in the incubation medium was either a bolus administration or a controlled release of an equivalent amount from microbeads within the size range of 50-200 µm made with ultrapurified low-viscosity high-guluronic acid (UP-LVG) alginate. USCs were incubated with or without HGF for 3 days or 7 days before removal of the incubation media, followed by harvesting sEV by the precipitation method. The protein content of isolated sEV was measured by bicinchoninic acid assay (BCA) for these three groups: control (no HGF beads), bolus HGF, and HGF beads. We also performed nanoparticle tracking analysis (NTA), Western blot assay, and ELISA for the HGF content of samples.</p><p><strong>Results: </strong>We found a significantly higher concentration of proteins in the HGF microbead group (control release group) compared to the bolus group and the control group after 7 days (<i>p</i> < 0.0017). The NTA data aligned with the BCA; they showed a significantly higher concentration of particles within the size range of sEV (<200 nm) in the group treated with HGF beads compared to the two other groups on day 7 (<i>p</i> < 0.0001).</p><p><strong>Conclusion: </strong>We found that administration of HGF to USCs by controlled release of the growth factor significantly enhances the levels of sEV secretion during 7 days of incubation.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of finite element modeling methods for predicting compression screw failure in a custom pelvic implant.","authors":"Yuhui Zhu, Ata Babazadeh-Naseri, Matthew R W Brake, John E Akin, Geng Li, Valerae O Lewis, Benjamin J Fregly","doi":"10.3389/fbioe.2024.1420870","DOIUrl":"10.3389/fbioe.2024.1420870","url":null,"abstract":"<p><p><b>Introduction:</b> Three-dimensional (3D)-printed custom pelvic implants have become a clinically viable option for patients undergoing pelvic cancer surgery with resection of the hip joint. However, increased clinical utilization has also necessitated improved implant durability, especially with regard to the compression screws used to secure the implant to remaining pelvic bone. This study evaluated six different finite element (FE) screw modeling methods for predicting compression screw pullout and fatigue failure in a custom pelvic implant secured to bone using nine compression screws. <b>Methods:</b> Three modeling methods (tied constraints (TIE), bolt load with constant force (BL-CF), and bolt load with constant length (BL-CL)) generated screw axial forces using functionality built into Abaqus FE software; while the remaining three modeling methods (isotropic pseudo-thermal field (ISO), orthotropic pseudo-thermal field (ORT), and equal-and-opposite force field (FOR)) generated screw axial forces using iterative physics-based relationships that can be implemented in any FE software. The ability of all six modeling methods to match specified screw pretension forces and predict screw pullout and fatigue failure was evaluated using an FE model of a custom pelvic implant with total hip replacement. The applied hip contact forces in the FE model were estimated at two locations in a gait cycle. For each of the nine screws in the custom implant FE model, likelihood of screw pullout failure was predicted using maximum screw axial force, while likelihood of screw fatigue failure was predicted using maximum von Mises stress. <b>Results:</b> The three iterative physics-based modeling methods and the non-iterative Abaqus BL-CL method produced nearly identical predictions for likelihood of screw pullout and fatigue failure, while the other two built-in Abaqus modeling methods yielded vastly different predictions. However, the Abaqus BL-CL method required the least computation time, largely because an iterative process was not needed to induce specified screw pretension forces. Of the three iterative methods, FOR required the fewest iterations and thus the least computation time. <b>Discussion:</b> These findings suggest that the BL-CL screw modeling method is the best option when Abaqus is used for predicting screw pullout and fatigue failure in custom pelvis prostheses, while the iterative physics-based FOR method is the best option if FE software other than Abaqus is used.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11372789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing a single-cell multi-omic analytic platform to characterize <i>ex vivo</i>-engineered T-cell therapy products.","authors":"Maryam Moshref, Jerry Hung-Hao Lo, Andrew McKay, Julien Camperi, Joseph Schroer, Norikiyo Ueno, Shu Wang, Saurabh Gulati, Somayeh Tarighat, Steffen Durinck, Ho Young Lee, Dayue Chen","doi":"10.3389/fbioe.2024.1417070","DOIUrl":"10.3389/fbioe.2024.1417070","url":null,"abstract":"<p><p>Genetically engineered CD8⁺ T cells are being explored for the treatment of various cancers. Analytical characterization represents a major challenge in the development of genetically engineered cell therapies, especially assessing the potential off-target editing and product heterogeneity. As conventional sequencing techniques only provide information at the bulk level, they are unable to detect off-target CRISPR translocation or editing events occurring in minor cell subpopulations. In this study, we report the analytical development of a single-cell multi-omics DNA and protein assay to characterize genetically engineered cell products for safety and genotoxicity assessment. We were able to quantify on-target edits, off-target events, and potential translocations at the targeting loci with per-cell granularity, providing important characterization data of the final cell product. Conclusion: A single-cell multi-omics approach provides the resolution required to understand the composition of cellular products and identify critical quality attributes (CQAs).</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11368872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usage of <i>Chlorella</i> and diverse microalgae for CO₂ capture - towards a bioenergy revolution.","authors":"Mohamed Ashour, Abdallah Tageldein Mansour, Yousef A Alkhamis, Mostafa Elshobary","doi":"10.3389/fbioe.2024.1387519","DOIUrl":"10.3389/fbioe.2024.1387519","url":null,"abstract":"<p><p>To address climate change threats to ecosystems and the global economy, sustainable solutions for reducing atmospheric carbon dioxide (CO₂) levels are crucial. Existing CO₂ capture projects face challenges like high costs and environmental risks. This review explores leveraging microalgae, specifically the <i>Chlorella</i> genus, for CO₂ capture and conversion into valuable bioenergy products like biohydrogen. The introduction section provides an overview of carbon pathways in microalgal cells and their role in CO₂ capture for biomass production. It discusses current carbon credit industries and projects, highlighting the <i>Chlorella</i> genus's carbon concentration mechanism (CCM) model for efficient CO₂ sequestration. Factors influencing microalgal CO₂ sequestration are examined, including pretreatment, pH, temperature, irradiation, nutrients, dissolved oxygen, and sources and concentrations of CO₂. The review explores microalgae as a feedstock for various bioenergy applications like biodiesel, biooil, bioethanol, biogas and biohydrogen production. Strategies for optimizing biohydrogen yield from <i>Chlorella</i> are highlighted. Outlining the possibilities of further optimizations the review concludes by suggesting that microalgae and <i>Chlorella</i>-based CO₂ capture is promising and offers contributions to achieve global climate goals.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11368733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}