Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Effects of workload on 3D joint moments in cycling and their implications for injury prevention.","authors":"Ezequiel Martín-Sosa, Juana Mayo, Joaquín Ojeda","doi":"10.3389/fbioe.2025.1657558","DOIUrl":"10.3389/fbioe.2025.1657558","url":null,"abstract":"<p><strong>Background and introduction: </strong>Several studies have been published in the literature analysing the effect of workload on joint moments during pedalling. Most of these studies focus on a single plane, mainly the sagittal plane, or on one joint (the knee). In this work a workload effect analysis is proposed on the three main joints of the lower body and on the three anatomical planes. Usually, this type of study has been carried out using 0D metrics such as the maximum or the variable range. In this work the analysis has been extended by complementing it with a statistical parametric mapping analysis.</p><p><strong>Methodology: </strong>Ten participants pedalling at 90 rpm and three pedal powers (170, 240 and 310 W) were analysed.</p><p><strong>Results: </strong>The results obtained show that pedalling power affects the moments in the three joints and in the three anatomical planes.</p><p><strong>Discussion: </strong>Analysis of the common causes of the main overload injuries that occur in cycling suggests that a three-dimensional analysis of the joint moments of the three main joints of the lower limb, including 0D and 1D data, is useful for the prevention of these injuries.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1657558"},"PeriodicalIF":4.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refillable silicone pump with precise switching for timed therapeutic delivery.","authors":"Naaz Thotathil, John J Amante, Micah Wingell, Grace W Hutter, Ultan Fallon, Yiling Fan, Keegan Mendez, Ellen T Roche, Cathal J Kearney","doi":"10.3389/fbioe.2025.1649771","DOIUrl":"10.3389/fbioe.2025.1649771","url":null,"abstract":"<p><strong>Introduction: </strong>Given the precise temporal coordination of natural biological processes, administering therapeutic agents at specific times can be used to enhance efficacy in a range of applications. To achieve such controlled drug delivery, various stimulus-responsive techniques (e.g., ultrasound, temperature changes, and electromagnetic radiation) have been developed. However, many of these current methods exhibit limitations, such as premature leakage prior to stimulus activation or delayed and prolonged responsiveness to stimuli. Our research introduces a soft robotic pressure-actuated drug delivery pump aimed at improving therapeutic efficacy through precisely-timed drug administration.</p><p><strong>Methods: </strong>This device utilizes silicone - a low-modulus material - for both the therapeutic reservoir and the actuation chamber to create a biocompatible and conformable interface, facilitating controlled drug release and offering the potential to be adapted as an implantable drug delivery system. Two ports in the actuation chamber allow the therapeutic reservoir to be refilled. We actuated the pressure reservoir of the device in the range of 28.5 - 59.8 mmHg and tested: the pressure-dependent release from the device; repeated release; baseline release, and the ability to deliver a wide-range of therapeutics.</p><p><strong>Results: </strong>Importantly, the system demonstrated a reliable On/Off mechanism - confirmed by actuating to ∼80% of opening pressure over 5 days - which addresses a key limitation in many existing technologies. In vitro, the device was used to deliver a range of therapeutics and had non-significant differences versus manual delivery of therapeutics in relevant assays: antibiotics (doxycycline; reduced E. coli viability by 49.6% vs. 49.8%); adeno-associated virus (AAV; transduced 73.5% vs. 76.2% of cells); dexamethasone (2D fibroblast scratch wound closure 50.9% vs. 51.0%); and successful delivery of viable cells (viability of 83% vs. 100%). We additionally developed a finite element model to model the pressure/volume release trend, and demonstrated the effect of membrane stiffness on release.</p><p><strong>Discussion: </strong>Our results demonstrate that the device can consistently administer therapeutics and molecules of various sizes and functions while maintaining their bioactivity, showcasing its potential for repeated, precisely-timed therapeutic delivery.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1649771"},"PeriodicalIF":4.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable cinnamaldehyde-loaded ZIF-8/Gallic Acid-Grafted gelatin hydrogel for enhanced angiogenesis and skin regeneration in diabetic wound healing.","authors":"Bo Zhou, Chen Zhang, Sheng Dai, Jin Zhao, Huaiyu Li, Yanbin Peng, Yunfeng Chu, Zhong Chen, Haotian Qin, Hui Zeng","doi":"10.3389/fbioe.2025.1660821","DOIUrl":"10.3389/fbioe.2025.1660821","url":null,"abstract":"<p><strong>Background: </strong>Chronic diabetic wounds remain a major clinical challenge due to persistent ischemia, oxidative stress, and impaired angiogenesis. Injectable hydrogels capable of adapting to irregular wound beds and delivering bioactive cues offer promising therapeutic potential for enhancing tissue regeneration.</p><p><strong>Methods: </strong>We developed a multifunctional injectable hydrogel by incorporating cinnamaldehyde-loaded ZIF-8 nanoparticles (CA@ZIF-8) into a gallic acid-grafted gelatin (GGA) matrix, followed by transglutaminase-mediated crosslinking. The physicochemical characteristics, drug release behavior, and mechanical performance of the CA@ZIF-8/GGA hydrogel were systematically evaluated. <i>In vitro</i> assays using human umbilical vein endothelial cells (HUVECs) were conducted to assess cytocompatibility and angiogenic activity. A full-thickness skin wound model in streptozotocin-induced diabetic rats was employed to evaluate <i>in vivo</i> wound healing efficacy and biocompatibility.</p><p><strong>Results: </strong>The CA@ZIF-8/GGA hydrogel exhibited favorable injectability, enhanced mechanical strength, and sustained release of both cinnamaldehyde and Zn²⁺. <i>In vitro</i>, the hydrogel significantly promoted HUVEC proliferation, migration, and tube formation, accompanied by upregulated expression of CD31 and VEGF. <i>In vivo</i>, CA(0.6)@ZIF-8/GGA-treated wounds demonstrated accelerated closure, enhanced granulation tissue formation, increased neovascularization, and re-epithelialization compared with control groups. No histological abnormalities were observed in major organs, indicating good systemic biocompatibility.</p><p><strong>Conclusion: </strong>This study presents an injectable CA@ZIF-8/GGA composite hydrogel that effectively promotes angiogenesis and diabetic wound regeneration. The synergistic integration of MOF-based controlled release and polyphenol-enhanced bioactivity highlights its potential as a clinically translatable platform for chronic wound management.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1660821"},"PeriodicalIF":4.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative review: clinical and pathological heterogeneity in knee <i>versus</i> temporomandibular joint osteoarthritis.","authors":"Jiannan Zhao, Ruoyi Wang, Songsong Zhu, Zhen Li, Rong Ren, Nan Jiang","doi":"10.3389/fbioe.2025.1684481","DOIUrl":"10.3389/fbioe.2025.1684481","url":null,"abstract":"<p><p>Temporomandibular joint osteoarthritis (TMJOA) remains a clinically underrecognized and insufficiently studied disorder, despite exerting a comparable impact on quality of life as knee osteoarthritis (knee OA). TMJOA can lead to chronic pain, limited mouth opening, joint dysfunction, and craniofacial deformities, yet it receives disproportionately less research attention and lacks standardized diagnostic and therapeutic frameworks. While TMJOA and knee OA share several hallmark pathological features-including cartilage degeneration, subchondral bone remodeling, and synovitis-these manifestations are shaped by joint-specific anatomical and biomechanical environments, resulting in distinct disease trajectories. Current evidence highlights that the pathogenesis of TMJOA remains poorly defined, with unresolved questions surrounding the role of mechanical loading in altering the cartilage microenvironment, the mechanisms underlying pathological calcification, and the influence of sex hormones such as estrogen and progesterone on disease onset and progression. In contrast, decades of knee OA research have yielded validated preclinical models, detailed molecular insights, and emerging regenerative strategies. This review systematically compares the two forms of osteoarthritis from clinical, anatomical, and pathological perspectives. We propose that TMJOA research may benefit significantly from cross-joint insights derived from the more extensively studied knee OA. Cross-comparative approaches not only provide a valuable framework for understanding joint-specific disease mechanisms but also offer new directions for the development of targeted therapies and diagnostic tools tailored to TMJOA. Bridging the current knowledge gap through interdisciplinary and translational research may ultimately improve outcomes for patients affected by this overlooked joint disease.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1684481"},"PeriodicalIF":4.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gait phase recognition of children with cerebral palsy via deep learning based on IMU data from a soft ankle exoskeleton.","authors":"Zhi Pang, Zewei Li, Ying Li, Bingshan Hu, Qiu Wang, Hongliu Yu, Wujing Cao","doi":"10.3389/fbioe.2025.1679812","DOIUrl":"10.3389/fbioe.2025.1679812","url":null,"abstract":"<p><p>Accurate gait-phase identification in children with Cerebral Palsy (CP) constitutes a pivotal prerequisite for evidence-based rehabilitation. Addressing the precise detection of gait disturbances under natural ambulation, we propose a deep-learning framework that integrates a stacked denoising autoencoder (SDA) with a long short-term memory network (SDA-LSTM) to classify four canonical gait phases. A community-oriented dataset was constructed by synchronizing ankle-mounted inertial measurement units (IMU) with plantar-pressure insoles; natural gait sequences of six children with mild CP were acquired in open environments. The SDA layer robustly extracts discriminative representations from non-stationary, high-noise signals, whereas the LSTM module models inter-phase temporal dependencies, thereby enhancing generalization cross-user. In noise-free conditions the SDA-LSTM framework attained 97.83% accuracy, significantly exceeding SVM (94.68%), random forest (96.05%), and standalone LSTM (95.86%). Under additive Gaussian noise with SNR ranging from 5 to 30 dB, the model preserved stable performance; at 10 dB SNR (Signal-to-Noise Ratio), accuracy remained 90.96%, corroborating its exceptional robustness. These findings demonstrate that SDA-LSTM effectively handles the complex, heterogeneous gait patterns of children with CP and is readily deployable for clinical assessment and exoskeletal assistance systems, indicating substantial translational potential.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1679812"},"PeriodicalIF":4.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> evaluation of methylglyoxal as an antibacterial additive to bone cement.","authors":"Maja Charlotte Bohn, Hilke Oltmanns, Jessica Meißner","doi":"10.3389/fbioe.2025.1661383","DOIUrl":"10.3389/fbioe.2025.1661383","url":null,"abstract":"<p><p>Periprosthetic joint infections (PJIs) are severe complications following surgical joint replacement and one of the main reasons for implant failure in human and veterinary medicine. Due to the global rise in antibiotic resistances and failure to prevent and treat PJIs, it is necessary to identify new antibacterial substances for the management of these infections. Methylglyoxal (MGO) is a dicarbonyl compound that has been identified as the main antibacterial component in Manuka honey. The aim of the study was to evaluate the suitability of MGO as an additive to polymethylmethacrylate bone cement in connection to PJIs. To test the antibacterial activity of pure MGO and MGO-containing bone cement against clinical isolates of <i>Staphylococcus (S.) pseudintermedius</i>, minimal inhibitory concentrations (MICs) were determined, growth of bacteria on bone cement was visualized, and the influence on infection of human osteosarcoma (HOS) cells was examined. Cytotoxicity of pure MGO and MGO-containing bone cement against HOS cells was analyzed with viability and proliferation assays, staining of cells on bone cement surface, and measurement of Interleukin-6 (IL-6) release. Activation of p38 MAP kinase was analyzed using Western blotting. MGO inhibited growth of <i>S. pseudintermedius</i> at 0.15 mg/mL, reduced bacterial colonization of bone cement at 25 mg per bone cement platelet, and reduced infection of HOS cells at 0.05 mg/mL. The IC₅₀ of pure MGO for cell viability was 0.17 mg/mL. At higher concentrations, bone cement with MGO reduced viability and proliferation, but did not cause IL-6 release. Western blots revealed p38 activation following MGO treatment, indicating involvement of the p38 pathway in stress reactions due to the treatment. Taken together, effectiveness of MGO against PJI-relevant <i>S. pseudintermedius</i> could be shown but biocompatibility was limited and further research is necessary to enhance biocompatibility.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1661383"},"PeriodicalIF":4.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Joint-level proprioceptive deficits and postural instability in Fibromyalgia: a biomechanical assessment using digital inclinometry and dynamic posturography.","authors":"Praveen Kumar Kandakurti, Ravi Shankar Reddy, Hani Hassan Alnakhli, Batool A Alkhamis, Ghada M Koura, Mohammad A ALMohiza, Faisal M Alyazedi, Debjani Mukherjee, Vikram Sreenivasa Rao","doi":"10.3389/fbioe.2025.1622679","DOIUrl":"10.3389/fbioe.2025.1622679","url":null,"abstract":"<p><strong>Objective: </strong>Fibromyalgia syndrome (FMS) is characterized by chronic musculoskeletal pain, fatigue, and sensory disturbances, often leading to impaired proprioception and postural control. This study aimed to examine joint reposition sense (JRS) at the hip, knee, and ankle, alongside limits of stability (LOS), in elderly individuals with FMS using digital inclinometers and computerized posturography.</p><p><strong>Methods: </strong>A total of 108 participants (54 with FMS, 54 age-matched healthy controls) were assessed. JRS was assessed at standardized joint angles of the hip (60° flexion), knee (45° flexion), and ankle (15° plantarflexion) using calibrated digital inclinometers, while LOS parameters-reaction time, maximum excursion, and directional control-were recorded with dynamic posturography.</p><p><strong>Results: </strong>Participants with FMS showed significantly higher joint position errors at the hip (mean difference = 2.53°), knee (2.51°), and ankle (2.24°) (p < 0.001, Cohen's d > 1.8). LOS parameters were also impaired in the FMS group, with slower reaction time (Δ = 0.97 s), reduced maximum excursion (Δ = -3.44%), and lower directional control (Δ = -22.64%) (all p < 0.001). JRS errors negatively correlated with LOS metrics, particularly at the hip and knee. Regression analysis confirmed JRS as a significant predictor of postural control.</p><p><strong>Conclusion: </strong>Lower limb proprioceptive deficits significantly impact postural stability in individuals with FMS. Targeted proprioceptive training, especially at the hip and knee, may improve functional balance and reduce fall risk in this population.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1622679"},"PeriodicalIF":4.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Cu-doped cotton textile against respiratory pathogens for preventing hospital-acquired infections.","authors":"Jianhui Gao, Deliang Liu, Zhiqiang Lin, Yang Zhou, Zhuojun He, Xiafei Dai, Pengfei Zhao, Hongzhou Lu, Mingbin Zheng","doi":"10.3389/fbioe.2025.1641123","DOIUrl":"10.3389/fbioe.2025.1641123","url":null,"abstract":"<p><p>Respiratory pathogens transmitted <i>via</i> clinical textiles represent a major source of hospital-acquired infections, yet current antibacterial fabric strategies are limited by poor durability and weak bacterial inhibition. Here, we reported a molecular-level strategy for the fabrication of copper-doped antibacterial cotton textiles (Cu@textile) <i>via</i> a simple immersion of common cotton in a Cu(II)-saturated NaOH solution. This process enabled stable coordination between the copper ions and cellulose hydroxyl groups, forming stable Cu-O bonds throughout the fiber matrix. Structural and spectroscopic analyses confirmed uniform copper integration and chemical bonding. The resulting Cu@textile exhibited potent, broad-spectrum antibacterial activity against key respiratory pathogens, including <i>Pseudomonas aeruginosa</i>, <i>Acinetobacter baumannii</i>, and <i>Mycobacterium tuberculosis</i>, with >99% sterilization efficiency. Mechanistic studies revealed this efficacy as copper-induced reactive oxygen species (ROS) production and bacterial membrane disruption. This accessible and scalable antimicrobial textile eliminates the need for specialized equipment or nanoparticle synthesis, and may represent a strategic intervention to reduce bacteria propagation and contact infection risks in healthcare settings.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1641123"},"PeriodicalIF":4.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing LDD diagnosis with YOLOv9-AID: simultaneous detection of pfirrmann grading, disc herniation, HIZ, and Schmorl's nodules.","authors":"Erling Xiang, Yongkang Zou, Jiale Chen, Jian Peng, Chunhai Huang, Feiwen Li, Xiaoping Li, Shenghua Qin, Zhiyu Li, Nanyu Li, Xu Zhou, Mingzheng Zhang","doi":"10.3389/fbioe.2025.1626299","DOIUrl":"10.3389/fbioe.2025.1626299","url":null,"abstract":"<p><p>This study develops an intelligent diagnostic model for LDD based on a novel YOLOv9-AID detection network and evaluates the impact of multiple innovative strategies on detection performance. A total of 222 adult patients who underwent lumbar MRI for low back pain or radicular leg pain were enrolled, yielding 1110 de-identified sagittal T2-weighted images (five per case). After excluding cases with prior spinal trauma, tumors, postoperative changes, congenital malformations, or severe artefacts, 202 cases (1,010 images) were randomly divided into training, validation, and internal test sets (8:1:1), while 20 cases (100 images) formed an external dataset for generalization assessment. The YOLOv9-AID model introduces three key enhancements to the baseline YOLOv9: a SlideLoss function to rebalance training weights between high- and low-quality samples; spatial-channel collaborative attention modules (SCSA) embedded at layers 5 and 11 to strengthen lesion feature extraction; and an ExtraDW-inspired redesign of the ResNCSPELAN4 module to boost precision and reduce parameter count. In the internal test set, the model achieved an mAP50 of 82.8% and an overall detection precision of 80.3%, with Schmorl's node detection at 92.9%, Pfirrmann grading accuracy at 93.3%, and disc herniation accuracy at 73.2% (an 8.4% improvement). Recall rates increased by approximately 5% on average, with Schmorl's node recall up 15.1%, Pfirrmann recall up 1.8%, and herniation recall improvements of up to 12.3%. External validation confirmed robust generalization, and detection rates for small lesions such as high-intensity zones and Schmorl's nodes significantly outperformed conventional methods. These results demonstrate that the YOLOv9-AID network, through the integration of SlideLoss and spatial-channel attention mechanisms, substantially enhances the accuracy and robustness of LDD detection on MRI and offers a promising tool to support clinical diagnosis efficiency and consistency.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1626299"},"PeriodicalIF":4.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel platelet-rich plasma/ hyaluronic acid lyophilized formulations for wound healing applications.","authors":"Antonella D'Agostino, Maria d'Agostino, Marta Nardini, Anita Muraglia, Celeste Di Meo, Maddalena Mastrogiacomo, Chiara Schiraldi","doi":"10.3389/fbioe.2025.1619633","DOIUrl":"10.3389/fbioe.2025.1619633","url":null,"abstract":"<p><p>Platelet-rich plasma (PRP) is a well-known biological product used in regenerative medicine. One of the limitations of its clinical use is the need for surgeons to obtain ready-to-use preparations. A lyophilized formulation with specific and reproducible levels of growth factors can, therefore, be considered a significant improvement for tissue regeneration. Therefore, it is of great interest to develop a formulation that enables the prolonged release of the growth factors and bioactive components present in PRP while improving the stability of these biomolecules during storage. To this aim, specific preparations obtained by mixing hyaluronic acid (HA) of low-medium molecular weight (56 or 200 kDa) with PRP were lyophilized to achieve an \"off-the-shelf\" product. These formulations were characterized from both biophysical and biological perspectives. Primary human dermal fibroblast proliferation and time-lapse scratch assays were performed on freshly lyophilized formulations and during storage at different temperatures (4°C and 25°C) to assess their biological activity upon resuspension for up to 6 months. Gene and protein expressions of collagen type 1 and elastin at time zero were evaluated. Platelet-Derived Growth Factor (PDGF)-BB and Vascular Endothelial Growth Factor (VEGF) releases were measured using the ELISA assay. All HA/PRP formulations were able to induce cell proliferation compared to PRP alone. HA/PRP formulations exhibited a superior repair rate compared to PRP in the scratch assay, confirming HA's ability to improve fibroblast migration. In the presence of HA, 80% of wound closure was achieved within 24 h, whereas PRP-treated samples reached approximately 60% of the repaired area. These data were supported by collagen and elastin expression levels. In <i>in vitro</i> wound healing assays, lyophilized HA/PRP products exhibited a superior effect compared to PRP alone at 3 months, but no significant improvement was found after 6 months. Prolonged storage needs very low temperatures to preserve PRP features (-20°C). In conclusion, we propose novel lyophilized HA/PRP formulations as promising products for topical and intra-dermic applications, especially for wound healing. The combination of HA as a biopolymer facilitates the slow release of growth factors contained in PRP while also allowing for a prolonged shelf life under cold-controlled conditions (4°C).</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1619633"},"PeriodicalIF":4.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}