Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Rapid molecular detection of <i>Senecavirus A</i> based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and CRISPR/Cas12a.","authors":"Chenghui Jiang, Huibao Wang, Rongxia Guo, Rui Yang, Xiaoming Li, Ping Liu, Jing Wang, Jincai Yang, Yanyan Chang, Qiaoying Zeng","doi":"10.3389/fbioe.2025.1451125","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1451125","url":null,"abstract":"<p><strong>Introduction: </strong><i>Senecavirus A</i> (SVA), an emerging vesicular pathogen, is responsible for porcine idiopathic vesicular disease (PIVD). This disease is closely associated with porcine vesicular disease and acute neonatal piglet mortality, presenting a substantial threat to the global swine industry. At present, the absence of effective drugs or vaccines for treating the disease makes accurate diagnosis of SVA of paramount importance for the effective prevention and control of the disease.</p><p><strong>Methods: </strong>In this study, we combined reverse transcription loop-mediated isothermal amplification (RT-LAMP) and Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated protein12a (CRISPR/Cas12a) using a dual-labelled fluorescence quencher or fluorescent biotin single-stranded DNA reporter molecule to develop two rapid, reliable, and portable visual SVA assays: RT-LAMP-Cas12a-FQ and RT-LAMP-Cas12a-FB.</p><p><strong>Results: </strong>The two methods exhibited comparable detection limits, with 9.6 copies/μL achieved in 40 and 45 minutes, respectively. They did not cross-react with non-target nucleic acids extracted from other related viruses and showed high specificity for SVA RNA detection. Furthermore, the methods demonstrated satisfactory performance in detecting 69 porcine adventitious samples, with no significant differences from that of quantitative reverse transcription polymerase chain reaction (RT-qPCR).</p><p><strong>Discussion: </strong>In summary, the RT-LAMP-Cas12a-FQ and RT-LAMP-Cas12a-FB methods developed are promising for early detection and routine surveillance of porcine SVA in resource-poor areas.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1451125"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic heterojunction effects in Ag₃PO₄/SnO₂ nanocomposites: a photocatalytic study on isoproturon degradation.","authors":"Rishi Ram, Bhawna, Sanjeev Kumar, Akanksha Gupta, Ravinder Kumar, Kashyap Kumar Dubey, Vinod Kumar","doi":"10.3389/fbioe.2025.1458965","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1458965","url":null,"abstract":"<p><strong>Introduction: </strong>Pesticides such as isoproturon are widely employed and represent a considerable environmental concern. The development of sustainable and efficient degrading techniques is crucial. Photocatalytic degradation employing semiconductor materials is a compelling solution. This study examines the synergistic advantages of heterojunction formation by synthesizing, characterizing, and improving the photocatalytic efficacy of Ag₃PO₄/SnO₂ nanocomposites for the degradation of isoproturon.</p><p><strong>Methods: </strong>The Ag₃PO₄/SnO₂ nanocomposite was characterised using powder X-ray diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Ultraviolet-Diffuse Reflectance Spectroscopy (UV-DRS) and X-ray Photoelectron Spectroscopy (XPS). The effective synthesis of the Ag₃PO₄/SnO₂ heterojunction was confirmed by characterization data from various techniques (PXRD, FTIR, SEM, UV-DRS, XPS).</p><p><strong>Results and discussion: </strong>Elemental mapping confirmed uniform distribution of O, P, Ag, and Sn. High-resolution mass spectrometry (HRMS) was employed to analyse degradation products. The Ag₃PO₄/SnO₂ nanocomposite exhibited improved photocatalytic degradation of isoproturon compared to its precursors. In contrast to 25% for pure SnO₂ and 41% for Ag₃PO₄, over 97% degradation was achieved using Ag₃PO₄/SnO₂ nanocomposite within 120 min of light irradiation under identical conditions. The synergistic effects of heterojunction formation significantly enhanced isoproturon degradation using the Ag₃PO₄/SnO₂ nanocomposite. The heterojunction reduces electron-hole recombination rate and enhances photogenerated charge carriers for degradation via effective charge separation. The improved photocatalytic activity is ascribed to the increased surface area of the nanocomposite. The analysis of HRMS data revealed the degradation products. The findings demonstrate the efficacy of Ag₃PO₄/SnO₂ nanocomposites as photocatalysts for environmental remediation, namely in the breakdown of pesticides.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1458965"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143993072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of energy metabolism in macrophages to block MPS for enhancing the chemotherapy efficacy.","authors":"Li Bin, Linlin Huang, Aiyu Chen, Yinyi Yang, Yanmei Zheng, Hanwen Zhang, Qinfang Zhang, Jiahui Zheng, Meiting Qiu, Xiajin Li, Yangbo Tan","doi":"10.3389/fbioe.2025.1549101","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1549101","url":null,"abstract":"<p><p>Various biological barriers hinder the effective use of administered nanoparticles, with the mononuclear phagocyte system (MPS) being a major obstacle to their <i>in vivo</i> efficacy. Glucose metabolism is an important factor for macrophages to perform MPS clearance <i>in vivo</i>. In this study, energy metabolism-blocking nanoparticles PEG-S-S-PLA@RGD @Dox@BAY876 (RPDB NPs) were developed to change drug distribution in the body, improving the efficacy of chemotherapy. First, BAY876 showed an excellent inhibition effects on macrophage energy metabolism <i>in vitro</i>. This inhibitory behavior of energy metabolism reduced the aggregation of nanoparticles in macrophages. Similarly, the migration capacity of macrophages was also limited by reduced energy metabolism. Second, the fluorescence distribution in the mice also showed that the fluorescence intensity of RPDB NPs in the liver was about 40% of that of RPD NPs, suggesting that reducing energy metabolism helps to downregulate the uptake of mononuclear phagocytic cell (MPS), and change the distribution of the drug <i>in vivo</i>. Furthermore, anti-tumor effects of RPDB NPs were evaluated both <i>in vivo</i> and <i>in vitro</i>. <i>In vivo</i>, RPDB nanomicelles inhibited breast cancer by up to 68.3%, higher than other administration groups. Moreover, the pathological section of tumor exhibited a significantly greater increase in cell apoptosis in RPDB NPs group. Hence, inhibition of macrophage energy metabolism is a promising approach to eliminate MPS effects, while also opening up a new window for the effective inhibition of tumors development and metastasis.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1549101"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical and biological properties of 3D printed bone tissue engineering scaffolds.","authors":"Mingxuan Wang, Yunpeng Xu, Luoxi Cao, Le Xiong, Depeng Shang, Yang Cong, Dan Zhao, Xiaowei Wei, Junlei Li, Dapeng Fu, Haoyi Lian, Zhenhua Zhao","doi":"10.3389/fbioe.2025.1545693","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1545693","url":null,"abstract":"<p><p>Bone defects have historically represented a significant challenge in clinical practice, with traditional surgical intervention remaining the gold standard for their management. However, due to the problem of the origin of autologous and allogeneic bone and the complex and diverse bone defects, traditional surgical methods sometimes cannot meet the treatment needs and expectations of patients. The development of bone tissue engineering and 3D printing technology provides new ideas for bone defect repair. Ideal bioscaffold materials must have good mechanical properties, biocompatibility, osteoinduction and bone conduction capabilities. Additionally, factors such as degradation rate, appropriate porosity and a sustained antibacterial effect must be taken into account. The combination of 3D printing technology and synthetic composite biomaterial scaffolds has become a well-established approach in the treatment of complex bone defects, offering innovative solutions for bone defect repair. The combined application of seed cells, signalling factors and biological scaffolds is also beneficial to improve the therapeutic effect of complex bone defects. This article will therefore examine some of the most commonly used 3D printing technologies for biological scaffolds and the most prevalent bioscaffold materials suitable for 3D printing. An analysis will be conducted on the mechanical and biological properties of these materials to elucidate their respective advantages and limitations.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1545693"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12010109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143983543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical factors that contribute to tibial periprosthetic fracture after cementless Oxford Unicompartmental Knee Replacement: a finite element analysis.","authors":"Xiaoyi Min, Laurence Marks, Stephen Mellon, Takafumi Hiranaka, David Murray","doi":"10.3389/fbioe.2025.1543792","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1543792","url":null,"abstract":"<p><strong>Background: </strong>Tibial periprosthetic fracture (TPF) is a severe complication of cementless Oxford Unicompartmental Knee Replacement (OUKR) with patient risk factors including small tibial size and tibia vara with an overhanging medial tibial condyle. Surgical factors also influence fracture but remain poorly defined. This finite element (FE) analysis study identified surgical risk factors for TPF after OUKR and determined the optimal tibial component positioning to minimise fracture risk.</p><p><strong>Methods: </strong>Knees in two very high-risk, small, bilateral OUKR patients who had a TPF in one knee and a good result in the other were studied with FE analysis. Each patient's unfractured tibia was used as a comparator to study surgical factors. The tibial geometries were segmented from the pre-operative CT scans and FE models were built with the tibial components implanted in their post-operative positions. The resections in the fractured and unfractured tibias were compared regarding their mediolateral position, distal-proximal position, internal-external rotation and varus-valgus orientation. Models of the TPF tibial resections in the contralateral sides were also built in both patients. The risk of TPF was assessed by examining the magnitude and location of the highest maximum principal stress.</p><p><strong>Results: </strong>In both patients, large differences were found in the position and orientation of the tibial components in the fractured and unfractured tibias with the components in the fractured tibias placed more medially and distally. Suboptimal saw cuts resulted in poor positioning of the tibial components and created very high local stresses in the bone, particularly anteriorly (157 MPa and 702 MPa in the fractured side vs. 49 MPa and 63 MPa in the unfractured side in patient 1 and 2 respectively), causing fractures.</p><p><strong>Conclusion: </strong>In small patients with marked tibia vara the surgery is unforgiving. To avoid fracture, the horizontal cut should be conservative, aiming for a 3 bearing, the vertical cut should abut the apex of the medial tibial spine, and extreme internal or external rotation should be avoided. The component should be aligned with the posterior cortex and should not overhang anteriorly. In addition, contrary to current recommendations, the tibial component should be placed in varus (about 5°).</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1543792"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced preoperative prediction of pancreatic fistula using radiomics and clinical features with SHAP visualization.","authors":"Yan Li, Kenzhen Zong, Yin Zhou, Yuan Sun, Yanyao Liu, Baoyong Zhou, Zhongjun Wu","doi":"10.3389/fbioe.2025.1510642","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1510642","url":null,"abstract":"<p><strong>Background: </strong>Clinically relevant postoperative pancreatic fistula (CR-POPF) represents a significant complication after pancreaticoduodenectomy (PD). Therefore, the early prediction of CR-POPF is of paramount importance. Based on above, this study sought to develop a CR-POPF prediction model that amalgamates radiomics and clinical features to predict CR-POPF, utilizing Shapley Additive explanations (SHAP) for visualization.</p><p><strong>Methods: </strong>Extensive radiomics features were extracted from preoperative enhanced Computed Tomography (CT) images of patients scheduled for PD. Subsequently, feature selection was performed using Least Absolute Shrinkage and Selection Operator (Lasso) regression and random forest (RF) algorithm to select pertinent radiomics and clinical features. Last, 15 CR-POPF prediction models were developed using five distinct machine learning (ML) predictors, based on selected radiomics features, selected clinical features, and a combination of both. Model performance was compared using DeLong's test for the area under the receiver operating characteristic curve (AUC) differences.</p><p><strong>Results: </strong>The CR-POPF prediction model based on the XGBoost predictor with the combination of the radiomics and clinical features selected by Lasso regression and RF exhibited superior performance among these 15 CR-POPF prediction models, achieving an accuracy of 0.85, an AUC of 0.93. DeLong's test showed statistically significant differences (<i>P</i> < 0.05) when compared to the radiomics-only and clinical-only models, with recall of 0.63, precision of 0.65, and F1 score of 0.64.</p><p><strong>Conclusion: </strong>The proposed CR-POPF prediction model based on the XGBoost predictor with the combination of the radiomics and clinical features selected by Lasso regression and RF can effectively predicting the CR-POPF and may provide strong support for early clinical management of CR-POPF.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1510642"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing therapeutic antibody production through amino acid-induced pH shift in Protein A affinity chromatography.","authors":"Senzhu Lin, Yue Wang","doi":"10.3389/fbioe.2025.1567923","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1567923","url":null,"abstract":"<p><p>Protein aggregation, denaturation, and loss of potency often occur during Protein A chromatography due to the harsh acidic conditions required for antibody elution. This study presents a pH shift-based elution strategy that effectively mitigates these issues by introducing amino acid-based elution buffers to create a milder elution environment and increase the final elution pH. By optimizing the combination of pre-elution and elution buffers, the elution pool pH was increased up to 7.2, significantly enhancing protein stability. Among various elution buffers tested, amino acids with non-polar or polar uncharged side chains-such as leucine, glycine, and serine-exhibited the most effective pH transition, resulting in 0.5-2.9 units pH shifts. Additionally, the use of 50 mM Bis-Tris, pH 7.2 as a pre-elution buffer demonstrated the highest capacity for stabilizing pH shifts. The scalability of this approach was validated using a 10 cm diameter column, where yields remained comparable to small-scale experiments, and elution pool stability was able to be maintained for 72 h at 26°C. These findings establish pH-shifting elution as a scalable, cost-effective method for improving the recovery and stability of low pH-unstable therapeutic antibodies in Protein A chromatography.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1567923"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autofluorescence lifetime imaging classifies human B and NK cell activation state.","authors":"Rebecca L Schmitz, Jeremiah M Riendeau, Kelsey E Tweed, Peter Rehani, Kayvan Samimi, Dan L Pham, Isabel Jones, Elizabeth M Maly, Emmanuel Contreras Guzman, Matthew H Forsberg, Ankita Shahi, Lucia Hockerman, Jose M Ayuso, Christian M Capitini, Alex J Walsh, Melissa C Skala","doi":"10.3389/fbioe.2025.1557021","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1557021","url":null,"abstract":"<p><p>New non-destructive tools with single-cell resolution are needed to reliably assess B cell and NK cell function for applications including adoptive cell therapy and immune profiling. Optical metabolic imaging (OMI) is a label-free method that measures the autofluorescence intensity and lifetime of the metabolic cofactors NAD(P)H and FAD to quantify metabolism at a single-cell level. Here, we demonstrate that OMI can resolve metabolic changes between primary human quiescent and IL-4/anti-CD40 activated B cells and between quiescent and IL-12/IL-15/IL-18 activated NK cells. We found that stimulated B and NK cells had an increased proportion of free compared to protein-bound NAD(P)H, a reduced redox state, and produced more lactate compared to control cells. The NAD(P)H mean fluorescence lifetime decreased in the stimulated B and NK cells compared to control cells. Random forest models classified B cells and NK cells according to activation state (CD69⁺) based on OMI variables with an accuracy of 93%. Our results show that autofluorescence lifetime imaging can accurately assess B and NK cell activation in a label-free, non-destructive manner.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1557021"},"PeriodicalIF":4.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological analysis of myocardial bridging leading to myocardial ischemia: myocardial coronary coupling.","authors":"Guanghao Yu, Zhaokai Ming, Dan Qiao, Zhiguo Cheng, Liandi Li, Wei Guo, Xiaoqiang Ye, Wei Ma, Guangxin Chen, Mingming Ren, Jian Xing, Wenchang Tan, Dongliang Zhao","doi":"10.3389/fbioe.2025.1559963","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1559963","url":null,"abstract":"<p><strong>Introduction: </strong>Myocardial bridge (MB) is a segment of an otherwise extramyocardial blood vessel that traverses the myocardium. This congenital condition typically lacks obvious clinical manifestations during adolescence. However, as individuals age, the accumulated myocardial pressure on the coronary arteries can lead to non-obstructive coronary ischemia, angina pectoris, and even heart failure. Early diagnosis is crucial for assessing the risk of cardiovascular events.</p><p><strong>Methods: </strong>This study performed a morphological analysis of MB in 75 patients using dual-source Computed Tomographic Angiography (CTA). Through geometric three-dimensional reconstruction, measurements and statistical analyses were conducted on muscle bridge length, depth, length-to-depth ratio, cross-sectional area, and coronary artery curvature.</p><p><strong>Results: </strong>This study explores the morphological differences among normal individuals, those with superficial MB, and those with deep MB during diastole and systole under varying conditions of myocardial coronary coupling. The study found that the compression degree is greatest in the deep MB group, with the average compression level being approximately 17 times that of normal individuals and about 4.6 times that of patients with superficial MB.</p><p><strong>Discussion: </strong>The differences in the average cross-sectional area are more significant than those in the minimum cross-sectional area. The depth of the MB is more closely related to the degree of compression, suggesting that clinical intervention and attention should be focused on deep MBs.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1559963"},"PeriodicalIF":4.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of elastic band and vibration training on muscle strength, balance, and mobility in older women with a history of falls: a randomised controlled trial.","authors":"Zhou Zhang, Weizhi Xiong, Heng Liu","doi":"10.3389/fbioe.2025.1525000","DOIUrl":"https://doi.org/10.3389/fbioe.2025.1525000","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effects of combined elastic band resistance training (EBRT) and whole-body vibration training (WBVT) on lower limb isokinetic muscle strength, balance and physical performance in older women with history of falls.</p><p><strong>Methods: </strong>A total of 102 older women with a history of falls was randomly assigned to the elastic band group (<i>n</i> = 28), vibration group (<i>n</i> = 28), elastic band plus vibration group (<i>n</i> = 28) and control group (<i>n</i> = 28) for a 12-week program. All groups performed identical exercises (half squats, static squats, left/right lunges) thrice weekly. The control group trained on a flat ground without elastic bands or vibration; the elastic band group used elastic bands on the flat ground, while the vibration and combined groups exercised on a vibration platform. Training parameters included 3 sets × 10 repetitions with 20-s rest intervals.</p><p><strong>Results: </strong>Post-intervention, knee flexion peak torque increased by 9.7% (<i>p</i> < 0.05). Total length (TL), elliptical area (EA), total offset index (TAI), timed up and go test (TUG) and five times sit-to-stand test (FTSST) decreased by 16.2%, 14.2%, 21.9%, 16.9% and 14.0%, respectively; and modified fall efficacy scale (MFES) increased by 17.6% (<i>p</i> < 0.01) in the elastic band group. Knee extension peak torque increased by 16.3% (<i>p</i> < 0.05). TL, EA, TAI, TUG and FTSST decreased by 14.8%, 12.2%, 18.9%, 12.3% and 11.5%, respectively; and MFES increased by 16.2% in the vibration group (<i>p</i> < 0.01). Hip and knee flexion and knee and ankle extension peak torque increased by 19.5%, 18.8%, 30.2% and 30.1% (<i>p</i> < 0.01), respectively, in the elastic band plus vibration group. TAI, TL, EA, TUG and FTSST decreased by 28.5%, 24.6%, 38.3%, 22.4% and 29.0%, respectively, and MFES increased by 42.1% in the elastic band plus vibration group (<i>p</i> < 0.01). The elastic band plus vibration group demonstrated greater improvements in knee and ankle strength compared to single interventions, while the elastic band and vibration groups showed smaller changes (<i>p</i> < 0.05). Additionally TL, EA, TAI, TUG, and FTSST all decreased, while MFES increased (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>The combined EBRT and WBVT can enhance knee and ankle isokinetic muscle strength, improve balance ability and physical performance and reduce fear of falls by a greater degree than single interventions. EBRT and WBVT had limited effects on improving lower limb isokinetic muscle strength but enhanced balance ability, physical performance, and reduced fear of falls in older women with a history of falls. Both training modalities showed similar effects.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1525000"},"PeriodicalIF":4.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}