Frontiers in Bioengineering and Biotechnology最新文献

{"title":"Microspheres for 3D bioprinting: a review of fabrication methods and applications.","authors":"Dmitri Karaman, Kira Williams, Jolene Phelps, Fynn La Boucan, Gretchen Lewandowski, Kerrin O'Grady, Bosco Yu, Stephanie M Willerth","doi":"10.3389/fbioe.2025.1551199","DOIUrl":"10.3389/fbioe.2025.1551199","url":null,"abstract":"<p><p>Bioprinting incorporates printable biomaterials into 3D printing to create intricate tissues that maintain a defined 3D structure while supporting the survival and function of relevant cell types. A major challenge in 3D bioprinting is tuning material properties to ensure compatibility with different types of cells, while accurately mimicking the physiological microenvironment. Developing novel bioinks tailored to specific applications can help address this challenge by combining various materials and additives to tune the bioink formulation. Microspheres - small spherical particles - can incorporate drugs or growth factors to enable their controlled release, encapsulate cells to provide protection during printing, and provide structural reinforcement to tune mechanical properties and enable complex architectures. The particles range in size from 1 to 1000 μm and can be tuned to meet desired functions by optimizing their mode of production and the materials used for fabrication. This review presents an overview of microsphere production methods and considerations for optimizing the production process. It then summarizes how microspheres have been used to date in bioprinting applications. Finally, the existing challenges associated with the creation and use of microspheres are discussed along with avenues for future research.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1551199"},"PeriodicalIF":4.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"hiPSC-derived cardiac fibroblasts dynamically enhance the mechanical function of hiPSC-derived cardiomyocytes on an engineered substrate.","authors":"Mitchell Josvai, Jodi Lawson, Harshal Kanade, Meghana Kalluri, Corey L Anderson, Jianhua Zhang, Alana Stempien, Lee L Eckhardt, Timothy J Kamp, Wendy C Crone","doi":"10.3389/fbioe.2025.1546483","DOIUrl":"10.3389/fbioe.2025.1546483","url":null,"abstract":"<p><strong>Introduction: </strong>Cardiac fibroblasts deposit and turnover the extracellular matrix in the heart, as well as secrete soluble factors that play critical roles in development, homeostasis, and disease. Coculture of CFs and human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CMs) enhances CM mechanical output, yet the mechanism remains unclear.</p><p><strong>Methods: </strong>Here, we use an <i>in vitro</i> engineered platform to compare the effects on CM mechanical function of direct CM-CF Coculture and soluble signaling alone through CF Conditioned Medium to a CM Only monoculture. Mechanical analysis is performed using digital image correlation and custom software to quantify the coordination and organization of CM contractile behavior.</p><p><strong>Results: </strong>CM-CF Coculture induces larger CM contractile strains, and an increased rate of spontaneous contraction compared to CM Only. Additionally, CM-CF Cocultures have increased contractile anisotropy and myofibril alignment and faster kinetics. The paracrine effects of fibroblast conditioned medium (FCM) are sufficient to induce larger contractile strains and faster contraction kinetics with these effects remaining after the removal of FCM. However, FCM does not influence CM spontaneous rate, contractile alignment, anisotropy, or relaxation kinetics compared to CM Only control.</p><p><strong>Discussion: </strong>These data suggest that hiPSC-CFs exert dynamic and multifactorial effects on the mechanical function of hiPSC-CMs and highlight the importance of CFs in both the native heart and <i>in vitro</i> cardiac models. Further, this work demonstrates the applicability of the coculture-conditioned medium-monoculture paradigm to decouple the effects of paracrine factor and cell-cell signaling on hiPSC-CM mechanical function and maturation.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1546483"},"PeriodicalIF":4.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validity and reliability of single camera markerless motion capture systems with RGB-D sensors for measuring shoulder range-of-motion: a systematic review.","authors":"Unhyung Lee, Suji Lee, Sung-A Kim, Yohwan Kim, Seunghoon Lee","doi":"10.3389/fbioe.2025.1570637","DOIUrl":"10.3389/fbioe.2025.1570637","url":null,"abstract":"<p><strong>Introduction: </strong>Assessing shoulder joint range-of-motion (ROM) is crucial for evaluating shoulder mobility but remains challenging due to its complexity. This review examined the potential of single-camera markerless motion capture systems with an RGB-depth (RGB-D) sensor for shoulder ROM measurements, focusing on their reliability and validity.</p><p><strong>Methods: </strong>We systematically searched nine databases through December 2022 for studies that evaluated the reliability and validity of single-camera markerless motion-capture systems in measuring simple (one-directional) and complex (multi-directional) shoulder movements. We extracted data on participant characteristics, device details, and measurement outcomes, and then assessed the methodological quality using the Consensus-Based Standards for the Selection of Health.</p><p><strong>Results: </strong>Of the 2,976 articles identified, 14 were included in this review. The findings indicate that intra-rater reliability findings across six studies were inconsistent, with simple movements like abduction and flexion demonstrating better reliability and less heterogeneity compared to complex movements. Validity assessments across 12 studies also showed inconsistency, with abduction and flexion measurements exhibiting higher validity than rotational movements. Studies focusing on simple movements reported good to excellent validity, particularly for abduction and flexion. Quality assessments using the COSMIN checklist revealed that the methodological quality varied across studies, ranging from inadequate to very good.</p><p><strong>Discussion: </strong>This systematic review suggests that RGB-D sensors show promise for measuring shoulder joint ROM, especially in simple movements like flexion and abduction. However, complex movements and inconsistencies limit their immediate clinical applicability, necessitating further high-quality research with advanced devices to ensure accurate and reliable assessments.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1570637"},"PeriodicalIF":4.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different pressure midfoot wraps on balance and proprioception in amateur basketball athletes.","authors":"Chengliang Wu, Shuai Zhang, Tao Wu, Sheng-Wei Jia, Zhaowei Chu, Fan Yang","doi":"10.3389/fbioe.2025.1560522","DOIUrl":"10.3389/fbioe.2025.1560522","url":null,"abstract":"<p><strong>Introduction: </strong>Ankle sprains are prevalent in basketball. This study sought to determine how midfoot wraps affect postural stability and ankle proprioception.</p><p><strong>Methods: </strong>Twenty-two amateur basketball athletes performed three single-leg balance tests (static, head-elevated static, and unstable foam pad) under four wrap conditions (no wrap, low, medium, and high pressure), and balance measures were taken using a force platform. Standing time, center of pressure dynamics, surface electromyographic of the supporting leg musculature were recorded. Ankle proprioception joint position matching error was assessed by a digital inclinometer.</p><p><strong>Results and discussion: </strong>Results indicated that during balance tests on foam padding, participants demonstrated significantly longer standing time when wearing low-pressure midfoot wraps, compared to high-pressure wraps (<i>F</i> (3,63) = 4.32, <i>p</i> = 0.008, <i>η</i> ² = 0.17). Wearing high-pressure wraps reduced anterior-posterior dynamic stability index variability (<i>F</i> (3,63) = 3.89, <i>p</i> = 0.044, <i>η</i> ² = 0.16), suggesting enhanced sagittal-plane control. Intriguingly, high-pressure conditions evidenced convergent activation trends between medial and lateral gastrocnemius (GM/GL ratio shift from 1.3 to 1.0), albeit without statistical significance (<i>p</i> > 0.05). No significant difference was detected in joint position sense in ankle dorsiflexion, plantarflexion, eversion and inversion between different wrap conditions (<i>p</i> > 0.05). These findings suggest that low-pressure midfoot wraps may improve balance through enhanced cutaneous feedback, while high-pressure wraps enhance anterior-posterior dynamic stability, providing biomechanically informed strategies for ankle injury prevention in basketball.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1560522"},"PeriodicalIF":4.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on rehabilitation robot control based on port-Hamiltonian systems and fatigue dissipation port compensation.","authors":"Jingjing Li, Zhen Chen, Jian Li, Hongyu Yan, Zhen Li, Minshan Feng, Jiawen Zhan, Liwei Shao","doi":"10.3389/fbioe.2025.1609548","DOIUrl":"10.3389/fbioe.2025.1609548","url":null,"abstract":"<p><strong>Introduction: </strong>Upper-limb rehabilitation robots have been demonstrated to effectively promote motor recovery in stroke patients. However, in active training modes, control instability may be induced by the nonlinear and time-varying characteristics of muscle fatigue, increasing the risks of physical human-robot interaction and ultimately limiting rehabilitation outcomes.</p><p><strong>Methods: </strong>A novel control strategy within the port-Hamiltonian framework, incorporating a dynamic muscle fatigue model. Fatigue levels were assessed in real time using surface electromyography (sEMG) signals and mapped to damping parameters in joint space, enabling the port-based modeling of fatigue-related energy dissipation. A hierarchical control architecture was constructed, consisting of outer-loop admittance control and inner-loop energy shaping.</p><p><strong>Results: </strong>Theoretical analysis confirmed that the closed-loop passivity of the system was preserved and stability was ensured. Experimental validation further showed that, compared to fixed damping parameters, the proposed fatigue compensation approach reduced muscle fatigue accumulation by 45% and increased training duration by 40%.</p><p><strong>Discussion: </strong>The proposed fatigue-adaptive control framework was shown to enhance the safety, effectiveness, and physiological adaptability of rehabilitation training. The integration of real-time sEMG feedback and port-Hamiltonian modeling offers a promising solution for personalized robotic rehabilitation.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1609548"},"PeriodicalIF":4.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innate immune pathway activated mesenchymal stromal cells improve function and histologic outcomes in a rodent osteoarthritis model.","authors":"Cody Plaisance, Lyndah Chow, Renata Impastato, Zoë J Williams, Isabella Sabino, Katie J Sikes, Kelly S Santangelo, Steven Dow, Lynn M Pezzanite","doi":"10.3389/fbioe.2025.1525969","DOIUrl":"10.3389/fbioe.2025.1525969","url":null,"abstract":"<p><strong>Introduction: </strong>Intra-articular administration of mesenchymal stromal cells (MSC) has demonstrated anti-inflammatory and chondroprotective activity in both preclinical models and in randomized clinical trials in patients with osteoarthritis (OA). Nonetheless, precedent from MSC studies in non-OA models suggests that the overall anti-inflammatory effectiveness of MSC can be improved by prior immune activation through cytokines or innate immune pathways.</p><p><strong>Methods: </strong>Therefore, in the current study, we determined whether activation of MSC by two different innate immune pathways (Toll-like receptor 3 (TLR3) pathway or Stimulator of Interferon Genes (STING) pathway could improve their effectiveness for intra-articular treatment of OA, using a murine destabilization of the medial meniscus (DMM) model. Outcome parameters included voluntary gait activity, joint histology and RNA transcriptomic analyses of synovial tissues.</p><p><strong>Results: </strong>We found that activation of MSC via either innate immune pathway improved functional voluntary movement outcomes compared to treatment with non-activated MSC. Moreover, cartilage integrity, including cartilage preservation, was significantly improved in mice receiving activated MSC, with greater benefits observed in animals treated with STING pathway-activated MSC compared to animals treated with non-activated MSC alone. Transcriptomic analysis of joint tissues revealed that treatment with activated MSC upregulated pathways associated with tissue remodeling, angiogenesis, and wound healing compared to tissues from animals treated with non-activated MSC.</p><p><strong>Discussion: </strong>These findings indicate therefore that innate immune activation of MSC prior to intra-articular delivery for treatment of OA can significantly improve functional gait activity and chondroprotective effects compared to non-activated MSC and suggest that this strategy could be evaluated clinically.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1525969"},"PeriodicalIF":4.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12138259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in volumetric bone mineral density after ACL reconstruction with single-bundle and double-bundle: a 1-year follow-up study using peripheral quantitative computed tomography.","authors":"Binbin Yin, Chaohua Fang, Ren Hai Feng, Jian Min Wu","doi":"10.3389/fbioe.2025.1606404","DOIUrl":"10.3389/fbioe.2025.1606404","url":null,"abstract":"<p><strong>Background: </strong>The purpose of this study is to quantify changes in volumetric bone mineral density (vBMD) in different regions of the tibial plateau after single-bundle (SB) and double-bundle (DB) anterior cruciate ligament reconstruction (ACLR).</p><p><strong>Methods: </strong>Twenty-six patients with diagnosed anterior cruciate ligament (ACL) rupture were allocated into SB (10) or DB group (16) and completed the last follow-up at 12 months. Peripheral quantitative computed tomography (pQCT) was performed before surgery and at 1, 3, 6, and 12 months after surgery. Four regions of interest (ROI) were identified 2 mm below the medial and lateral subchondral plate of the knee joint, namely, the anteromedial (AM), posteromedial (PM), anterolateral (AL) and posterolateral (PL) regions. The vBMD of each ROI was measured and compared between the groups at different timepoints.</p><p><strong>Results: </strong>For the vBMD, a significant change in all ROIs can be found over time, with the values at all ROIs decreased until 6 months postoperatively and then steadily increased, but the values did not reach preoperative levels. The AM region had the highest vBMD, followed by the PL region, and the vBMD was lowest in the AL region. For the change percent, the decrease magnitude was comparable between AM and PM region at postoperative 1 and 12 months, but it was greater at PM region at postoperative 3 and 6 months. Comparing DB ACLR and SB ACLR, there was no significant difference in the change percent of vBMD in all ROIs, except for in the AL region at 1 month postoperatively.</p><p><strong>Conclusion: </strong>A partially reversible decline in vBMD was found in the proximal tibia at 12 months after ACLR. But the change percent varied among the different regions, which may indicate inadequate restoration of knee kinematics after ACLR. DB and SB ACLR methods may have a similar effect on knee kinematics.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1606404"},"PeriodicalIF":4.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regional bone density patterns of the tibial plateau: implications for finite element analysis.","authors":"Pablo Besa, Anselmo Alegría, Nicolás González, Fiorella Biancardi, Catalina Vidal, Pablo Cikutovic, Marcelo E Andia, Joaquín Mura","doi":"10.3389/fbioe.2025.1541536","DOIUrl":"10.3389/fbioe.2025.1541536","url":null,"abstract":"<p><p>The tibial plateau has different anatomical regions and heterogeneous bone densities. Most finite element simulation (FEM) studies of tibial plateau fracture fail to account these regional variations, which may significantly influence biomechanical behavior. This study aimed to quantify the regional density profile of the tibial plateau using Hounsfield Units (HU) from computed tomography (CT) scans and explore associations between density, age, and sex. We developed a novel measurement protocol to compare HU values of the subchondral bone and cancellous bone in eight different regions of the tibial plateau. Results demonstrated that patient age and female sex were associated with reduced bone density. Subchondral bone and medial bone had significantly higher density than metaphyseal and lateral bone, respectively. This findings could have implications on orthopedic modeling of tibial plateau fractures using FEM. Current FEM should consider distinct regions in tibial plateau to improve accuracy. Conclusion: Tibial plateau heterogenous bone density distribution could contribute to explain the low predictive accuracy in FEM models.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1541536"},"PeriodicalIF":4.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach with modified suture bridge fixation technique for posterior cruciate ligament tibial avulsion fracture in adult.","authors":"Xiong Wang, Qing Gu, Shuming Zi, Wenqiang Wei, Biao Cheng, Liehu Cao","doi":"10.3389/fbioe.2025.1496728","DOIUrl":"10.3389/fbioe.2025.1496728","url":null,"abstract":"<p><strong>Background: </strong>Posterior cruciate ligament (PCL) tibial avulsion fractures are relatively rare injuries that often result in chronic pain, limited range of motion, knee instability, and osteoarthritis. Most cases require surgical intervention to restore the fragment's normal anatomy, the ligament's tension, and the knee joint's stability. In this study, we propose a novel approach utilizing a modified suture bridge fixation technique to treat PCL tibial avulsion fractures and explore the clinical outcome and applicability.</p><p><strong>Methods: </strong>We retrospectively reviewed and collected the clinical data from March 2020 to April 2023. Of the 24 patients (14 males and 10 females) with PCL tibial avulsion fractures who underwent modified suture bridge fixation technique were enrolled in the study. The surgical data of the surgery time and intraoperative blood loss were analyzed. The knee range of motion (ROM), the Lysholm knee scoring scale, and the International Knee Documentation Committee (IKDC) were used to evaluate the recovery of knee joint function.</p><p><strong>Results: </strong>All 24 patients were followed up for a duration ranging from 11 to 16 months, with an average of 13.00 ± 1.32 months. The surgery time was 40∼60 min, with a mean of 50.88 ± 4.85 min. The intraoperative blood loss was approximately 25∼45 mL, averaging 36.75 ± 4.89 mL. No instances of wound infection, neurovascular injuries, fracture nonunion, fixation failure, deep vein thrombosis, or secondary operation were reported during follow-up. The knee joint range of motion (ROM) was 118°∼134°, with an average of 127.46° ± 4.16° at the final follow-up. The Lysholm score was 41.17 ± 3.48 at the preoperative stage and improved to 90.25 ± 2.59 at the final follow-up. The IKDC score was 40.38 ± 2.16 at the preoperative stage, and 88.54 ± 1.77 at the final follow-up.</p><p><strong>Conclusion: </strong>The results indicate that the novel approach utilizing a modified suture bridge fixation technique can provide effective stabilization and favorable clinical outcomes. The suture bridge structure can be applied to displaced posterior cruciate ligament (PCL) tibial avulsion fractures through its compression capabilities, especially in comminuted fractures. This procedure is straightforward, minimizing the risk of injury to peripheral neurovascular structures and eliminating the need for a second operation. Consequently, this technique represents a viable alternative treatment option for primary care facilities or hospitals that lack arthroscopic equipment.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1496728"},"PeriodicalIF":4.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cementless long-stem fixation in periprosthetic clamshell fracture: a biomechanical investigation.","authors":"Lingqi Zhu, Li Xie, Mingchang He, Jianyun Miao, Liang Zhou, Lianshui Huang, Hui Liu, Wei Xie, Wenliang Zhai","doi":"10.3389/fbioe.2025.1604441","DOIUrl":"10.3389/fbioe.2025.1604441","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the biomechanical strength of cementless long-stem fixation for the treatment of periprosthetic clamshell fracture.</p><p><strong>Methods: </strong>Eighteen Sawbones artificial femur models were used and divided into three groups. Group A had an intact medial wall. Group B, the proximal periprosthetic femoral medial wall was osteotomized to simulate periprosthetic clamshell fractures. Group C, following identical osteotomy to Group B, cerclage wiring was applied to fix the medial wall fracture fragment. After molding, the cementless long-stem were implanted in all models, which were then evaluated through axial compression tests, torsion tests, and axial failure tests. The axial stiffness, axial displacement, torsional stiffness, torque, and maximum failure load were recorded and statistically analyzed.</p><p><strong>Results: </strong>In the axial compression tests, no statistically significant differences were observed among Groups A, B, and C in terms of axial displacement, axial stiffness, or maximum failure load (the displacement greater than 3 mm). However, in the torsional tests, Group B showed significantly lower torsional stiffness or torque compared to Groups A and C (<i>p</i> < 0.05). No significant differences in torsional stiffness or torque were detected between Groups A and C.</p><p><strong>Conclusion: </strong>The integrity of the femoral medial wall is crucial for femoral stem stability. In case of medial wall fracture, while cementless long-stem implantation can achieve satisfactory axial stability, its torsional stability remains inadequate. The application of supplemental cerclage wiring for medial wall fragment fixation provides reliable improvement in torsional stability of the stem.</p>","PeriodicalId":12444,"journal":{"name":"Frontiers in Bioengineering and Biotechnology","volume":"13 ","pages":"1604441"},"PeriodicalIF":4.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12137322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}