Fitoterapia最新文献

{"title":"Auslactones A−E, austalide meroterpenoids from Aspergillus ustus NRRL 275","authors":"Lin Liu ,&nbsp;Yinhui Zhou ,&nbsp;Shu-Ming Li ,&nbsp;Hanli Ruan","doi":"10.1016/j.fitote.2026.107105","DOIUrl":"10.1016/j.fitote.2026.107105","url":null,"abstract":"<div><div>Five new austalide meroterpenoids, named auslactones A − E (<strong>1</strong>–<strong>5</strong>), one new natural product (<strong>6</strong>), together with ten known analogues (<strong>7</strong>–<strong>16</strong>), were isolated from the fungus <em>Aspergillus ustus</em> NRRL 275. Auslactones A − B (<strong>1</strong>–<strong>2</strong>) represent a class of austalides containing 5/6/6/6/6/5 hexacyclic ring with a hydroxymethyl group at C-15. Auslactones C − D (<strong>3</strong>–<strong>4</strong>) enrich the type of 5/6/6/6/5/6/5 heptacyclic austalides. Auslactone E (<strong>5</strong>) contains 5/6/6/6 tetracyclic ring system. Meanwhile, the NMR data of auslactone F (<strong>6</strong>) were reported for the first time. Their structures were elucidated by extensive spectroscopic analysis. The absolute configuration of compound <strong>1</strong> was established by single-crystal X-ray diffraction, whereas those for the others were established by circular dichroism (CD) data analysis. No cytotoxic or immunosuppressive activity was found for compounds <strong>1</strong>–<strong>6</strong>.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107105"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146040777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vasorelaxation and antihypertensive activity of standardized extract of Gymnema sylvestre in rodent model","authors":"Poonam Rani ,&nbsp;Deepak Kumar ,&nbsp;Kumari Savita ,&nbsp;Divya Mishra ,&nbsp;Shweta Parashar ,&nbsp;Palak Singh ,&nbsp;Manisha Yadav ,&nbsp;Feroz Khan ,&nbsp;Madhav Nilakath Mugale ,&nbsp;Prasant Kumar Rout ,&nbsp;Debabrata Chanda","doi":"10.1016/j.fitote.2026.107111","DOIUrl":"10.1016/j.fitote.2026.107111","url":null,"abstract":"<div><div><em>Gymnema sylvestre</em> (GS) leaf extract is one of the most important functional food indicated for management of diabetes, obesity and associated disorders and is also indicated in Ayurveda for heart diseases. Although the plant is currently under intense exploration for its therapeutic potential in cardiometabolic diseases but efficacy against hypertension and in inducing vasorelaxation in vasculature was never well explored. The present study envisaged vasorelaxation and antihypertensive activity of biomarkers enriched extract (Gym-cit) in rodent model. When given at 30 mg kg⁻¹, 100 mg kg⁻¹ and 300 mg kg⁻¹ once orally for 14-days, Gym-cit significantly decreased the systolic, diastolic and mean arterial pressure in SHR. GS biomarkers like Gymnemagenin (GMN), Deacyl gymnemic acid (DAC), Gymnemic acid IV (GA IV) and standardized extract exhibited concentration dependent vasorelaxation in <em>ex-vivo</em> model in mesenteric artery. Gymnemagenin (GMN) exhibited maximum vasorelaxation potential (E_max; 59.44 ± 1.88; <em>n</em> = 6), putatively through opening of BK_Ca channel and blocking of L-type voltage dependent calcium channel in vascular smooth muscle. Gym-cit showed decent vasorelaxation response and significant antihypertensive activity in SHRs with significant decrease in systolic, diastolic and mean arterial pressure. The study suggests great promise in Gym-cit against cardiometabolic diseases associated with hypertension.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107111"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146043755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new antiviral isorhamnetin glycoside from the Tunisian plant Herniaria hirsuta L.","authors":"Hana Slimi ,&nbsp;Hayet Edziri ,&nbsp;Fatma Nouira ,&nbsp;Lamjed Bouslama ,&nbsp;Maha Mastouri ,&nbsp;Adele Cutignano","doi":"10.1016/j.fitote.2026.107092","DOIUrl":"10.1016/j.fitote.2026.107092","url":null,"abstract":"<div><div><em>Herniaria hirsuta</em> L. (Caryophyllaceae) is an annual herb known as hairy rupturewort. It is used in traditional medicine, particularly in North Africa, to prevent and cure kidney stones and as a diuretic. Rich in saponins, it possesses many other bioactive secondary metabolites including flavonoids and their glycosides. Plant specimens harvested in Tunisia were chemically investigated and their extracts tested for antiviral activity. Ethyl acetate and butanol extracts showed significant activity against Herpes Simplex type-2 (HSV-2) virus (IC₅₀ = 31.15 μg/mL, SI =10.97 and IC₅₀ = 46.04 μg/mL, SI = 6.44, respectively). Ultra-High Performance Liquid Chromatography-High Resolution ElectroSpray Ionization Mass Spectrometry (UHPLC-HRESIMS) analyses combined with Nuclear Magnetic Resonance (NMR) spectroscopy revealed the occurrence of a family of isorhamnetin glycosides, containing 1 to 3 sugar units. By using a bioassay guided fractionation approach, we identified a new flavonoid glycoside: its chemical structure was resolved by extensive use of NMR spectroscopy and HRESIMS/MS as isorhamnetin-3-<em>O</em>-(2-<em>O</em>-(4-acetyl)-α-L-rhamnopyranosyl, 6-<em>O</em>-α-L-rhamnopyranosyl)-β-D-galactopyranoside, which exhibited an IC₅₀ = 20.34 μM (SI &gt;15). Notably, its co-occurring deacetyl derivative resulted inactive. <em>In vitro</em> assays suggested that the antiviral action is exerted during the early stages of the viral cycle, preventing HSV-2 from infecting target cells.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107092"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145965741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-faceted bioactivity of three unexplored Turkish plants: A comparative phytochemical and pharmacological profiling","authors":"Nilofar ,&nbsp;Gunes Ak ,&nbsp;Enver Saka ,&nbsp;Gokhan Zengin ,&nbsp;Cziaky Zoltan ,&nbsp;Josef Jeko ,&nbsp;Annalisa Chiavaroli ,&nbsp;Simonetta Cristina Di Simone ,&nbsp;Mariachiara Gabriele ,&nbsp;Maria Loreta Libero ,&nbsp;Giustino Orlando ,&nbsp;Luigi Menghini ,&nbsp;Claudio Ferrante ,&nbsp;Jasmina Glamočlija ,&nbsp;Mladen Rajaković ,&nbsp;Evren Yildiztugay ,&nbsp;Meng-Yao Li","doi":"10.1016/j.fitote.2025.107066","DOIUrl":"10.1016/j.fitote.2025.107066","url":null,"abstract":"<div><div>Since ancient times, plants have been used as a valuable source for treating serious diseases. Three different plants, namely <em>Aubrieta pinardii</em>, <em>Johrenia dichotoma</em>, and <em>Malabaila dasyantha</em> were used and extracted using the extraction solvents ethyl acetate (EA), methanol (MeOH), and water. Antioxidant activity was determined using several in vitro assays. Subsequently, the enzyme inhibition activities of the extracts were evaluated against cholinesterases (AChE and BChE), tyrosinase, amylase, and glucosidase. The antibacterial and antifungal effects of the extracts were assessed. Methanol extracts of <em>A</em>. <em>pinardii</em>, <em>J</em>. <em>dichotoma</em>, and <em>M</em>. <em>dasyantha</em> yielded the highest total flavonoids (TFC) compared to EA and water extracts. The analysis using HPLC-ESI-Q-TOF-MS identified various classes of phytochemicals within the species examined. <em>Aubrieta pinardii</em> extracts were abundant in glucosinolates, whereas <em>J. dichotoma</em> and <em>M. dasyantha</em> extracts were characterized by the presence of coumarins and furanocoumarins. The EA extracts exhibited greater inhibition of microorganism growth compared with MeOH and water extracts. Notably, <em>J. dichotoma</em> showed similar or superior antifungal activity against <em>A. niger</em> (MIC/MFC 0.25/0.50 mg/mL) and <em>M. dasyantha</em> demonstrated comparable efficacy against <em>T. viride</em> (MIC/MFC 0.50/1.00 mg/mL) and <em>P. verrucosum</em> var. <em>cyclopium</em> (MIC/MFC 0.125/0.25 mg/mL) when benchmarked against the reference drug ketoconazole. These results provide valuable information about <em>A</em>. <em>pinardii</em>, <em>J</em>. <em>dichotoma</em>, and <em>M</em>. <em>dasyantha</em> chemical profiles and underline the importance of selecting appropriate solvents for the extraction of metabolites from plant matrix for potential applications in the treatment of various diseases.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107066"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145846318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quinoline and para-aminobenzoic acid derivatives with anti-osteoclastic activity from a marine mesophotic zone Demosponge sponge-associated fungus Xylaria sp. NBUF245","authors":"Yu-Ying Liu ,&nbsp;Chan-Ting Lai ,&nbsp;Salman Khan ,&nbsp;Qian-Jing Zheng ,&nbsp;Rong Zhang ,&nbsp;Wei-Yi Wang ,&nbsp;Bin Wu ,&nbsp;Shan He ,&nbsp;Li Yang ,&nbsp;Ting-Ting Wang","doi":"10.1016/j.fitote.2025.107046","DOIUrl":"10.1016/j.fitote.2025.107046","url":null,"abstract":"<div><div>One undescribed quinoline derivative, xylaminoquin A (<strong>1</strong>), one new <em>p</em>-aminobenzoic acid derivative, xylamidate B (<strong>2</strong>), and methyl 4-(butanoylamino)-3-hydroxybenzoate, reported for the first time as a natural product and named xylamidate C (<strong>3</strong>), together with known alkaloids and diketopiperazines, methyl 4-acetylamino-3-hydroxybenzoate (<strong>4</strong>), methyl 4-[(1-oxobutyl)amino]benzoate (<strong>5</strong>), pseurotin A (<strong>6</strong>), azaspirofuran A (<strong>7</strong>), cyclo(<span>l</span>-Tyr-<span>l</span>-Leu) (<strong>8</strong>), and cyclo-(<span>l</span>-Tyr-<span>l</span>-Val) (<strong>9</strong>) were isolated from <em>Xylaria</em> sp. NBUF245, a fungus associated with a <em>Demosponge</em> sp. collected from the marine mesophotic zone. Structurally, compound <strong>1</strong> is the first example of 3,7-dissubstitented quinoline derivative isolated from fungi. The structures of all compounds were elucidated by combined analysis of spectroscopic data and X-ray crystallography. Bioassay results showed that compound <strong>5</strong> effectively prevented prednisolone-induced bone loss in zebrafish at a concentration of 30 μM, suggesting potential osteoprotective activity.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107046"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145833376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nine feruloyltyramine derivatives and one pyrrole with hepatoprotective activity isolated from fresh Portulaca oleracea L.","authors":"Hongqing Wang ,&nbsp;Yaru Jiao ,&nbsp;Jianbo Liu,&nbsp;Junhua Sun,&nbsp;Zhanrong Zhou,&nbsp;Ruoyun Chen,&nbsp;Jie Kang","doi":"10.1016/j.fitote.2026.107098","DOIUrl":"10.1016/j.fitote.2026.107098","url":null,"abstract":"<div><div>Nine feruloyltyramine derivatives (<strong>1</strong>, <strong>2</strong>, <strong>4</strong>–<strong>10</strong>) and one pyrrole (<strong>3</strong>) were isolated from 70% EtOH extract of fresh <em>Portulaca oleracea</em> L.. Among them, compounds <strong>1</strong>, <strong>2</strong> and <strong>4</strong> were cyclized feruloyltyramine derivatives, the other feruloyltyramine derivatives (<strong>5</strong>–<strong>10</strong>) were non-cyclized. Compounds <strong>1</strong> and <strong>2</strong> were racemic mixtures, and were separated into enantiomers <strong>1</strong>-<strong>a</strong> (<em>R</em>), <strong>1</strong>-<strong>b</strong> (<em>S</em>), <strong>2</strong>-<strong>a</strong> (<em>R</em>) and <strong>2</strong>-<strong>b</strong> (<em>S</em>), respectively. Three compounds (<strong>1</strong>–<strong>3</strong>) were new, and their structures were elucidated by UV, IR, HRESIMS, 1D and 2D NMR, and ECD spectra, as well as optical rotations. All the compounds (<strong>1</strong>−<strong>10</strong>) were tested for their hepatoprotective activity <em>in vitro</em>. At a concentration of 10 <em>μ</em>M, three cyclized feruloyltyramine derivatives (<strong>1</strong>-<strong>a</strong>, <strong>2</strong>-<strong>a</strong> and <strong>4</strong>) possessed significant hepatoprotective activity against the damage induced by <em>N</em>-acetyl-para-aminophenol (APAP) in human HepG2 liver cancer cells, with cell survival rates of 63.61, 65.44 and 65.52%. It was suggested that the cyclized feruloyltyramine derivatives with <em>R</em> configuration had better hepatoprotective activity than that of <em>S</em> configuration and non-cyclized feruloyltyramine derivatives.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107098"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146009459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel diterpenoid glycosides from Rubus chingii Hu induce apoptosis in BPH-1 cells through fas/caspase-8 pathway activation","authors":"Jing Wang ,&nbsp;Xue Zhang ,&nbsp;Lu Chen ,&nbsp;Jun Du ,&nbsp;Xiaohong Wu ,&nbsp;Liang Chen ,&nbsp;Yingchun Wu ,&nbsp;YiMing Li","doi":"10.1016/j.fitote.2025.107069","DOIUrl":"10.1016/j.fitote.2025.107069","url":null,"abstract":"<div><div><em>Rubus chingii</em> Hu, a food material with medicinal value, has been used to treat urinary system diseases, such as prostatitis, benign prostate hyperplasia (BPH) and lower urinary tract symptoms (LUTS). However, there is no research on the efficacy and mechanism of compounds of <em>R. chingii</em>. in treating BPH. In this study, 10 previously unreported <em>ent</em>-pimarane-type (rubuschinoside A–B, <strong>1</strong>–<strong>2</strong>), <em>ent</em>-isopimarane-type (rubuschinoside C<img>D, <strong>3</strong>–<strong>4</strong>) an<em>d ent-</em>labdane<em>-</em>type (goshonoside J16–21, <strong>5</strong>–<strong>10</strong>) diterpene glycosides were isolated from the dried fruits of <em>R. chingii</em>. Their structures were elucidated and confirmed using high-resolution electrospray ionization mass spectrometry (HRESIMS), nuclear magnetic resonance spectroscopy (NMR), single crystal X-ray diffraction and electron capture detector (ECD). All compounds were tested for their apoptosis-inducing activity in BPH cells, and compounds <strong>1</strong>, <strong>3</strong>, <strong>4</strong> and <strong>8</strong> exhibited significant pro-apoptotic effects. Further apoptosis mechanistic studies on compound <strong>8</strong> revealed that it could increase the expression of Fas, FADD, caspase 3 and caspase 8 mRNA expression levels at 50 μM. Collectively, this study suggested that diterpenoid glycosides from <em>R. Chingii</em> may induce apoptosis in hyperplastic prostate cells through activation of the Fas/caspase-8 signaling cascade.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107069"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of series reactive dialdehyde intermediates by metabolism of monotropein","authors":"Xiaoli Li ,&nbsp;Zejun Liu ,&nbsp;Qiuyi Jing ,&nbsp;Hong Pan ,&nbsp;Fuguo Shi","doi":"10.1016/j.fitote.2025.107072","DOIUrl":"10.1016/j.fitote.2025.107072","url":null,"abstract":"<div><div>Monotropein, an iridoid glycoside, is the principal bioactive constituent with the highest amount isolated from <em>Morindae officinalis</em> radix, which has been reported to have potent pharmacological properties. Monotropein contains a hemiacetal structure. The hemiacetal may be converted into a reactive dialdehyde intermediate and covalently bind to the primary amine. Therefore, we speculated that the metabolites of monotropein with hemiacetal can generate dialdehyde intermediates, a class of highly reactive metabolites that enable covalent modification of proteins. This study characterized the metabolic profiling of monotropein, especially the unstable dialdehyde intermediates, using UPLC-Q/Orbitrap MS. A total of eighteen stable metabolites were characterized in monotropein-treated rats. The main metabolic pathways were dehydration, deglycosylation, and decarboxylation. It is noteworthy that six unstable dialdehyde intermediates of monotropein were characterized for the first time. They were the hemiacetal ring-opening products of M4 (monotropein aglycone), M5 (decarboxylated aglycone), M6 (methylated aglycone), M7 (dehydrated aglycone), M8 (hydrogenated aglycone), and M9 (dehydroxymethyl aglycone). The dialdehyde intermediates were identified by their stable dihydropyridine conjugates with amino acids. Five dialdehyde intermediates enabled covalent modification of proteins in the intestine. In conclusion, eighteen stable metabolites and six reactive dialdehyde intermediates were characterized in this study, which contributes to understanding the pharmacological effects of monotropein.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107072"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gu-Jin-Xiao-Ji Decoction potentiates anti-PD-1 immunotherapy in lung cancer by inhibiting the PI3K/AKT/NF-κB/PD-L1 axis","authors":"Yuxin Xie ,&nbsp;Gaoyang Lin ,&nbsp;Chuanlong Guo ,&nbsp;Jin Tian ,&nbsp;Lin Long ,&nbsp;Lili Zhao ,&nbsp;Longjiang Huang ,&nbsp;Hongmei Wei ,&nbsp;Jun Xiao ,&nbsp;Xuemao Liu","doi":"10.1016/j.fitote.2026.107085","DOIUrl":"10.1016/j.fitote.2026.107085","url":null,"abstract":"<div><div>This study aims to elucidate the molecular mechanisms by which the traditional Chinese medicine Gu-Jin-Xiao-Ji Decoction (GJXJD) enhances the anti-tumor efficacy of PD-1 inhibitors in lung cancer and to characterize its major chemical components. The major components of GJXJD were identified and quantitatively analyzed using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). A mouse Lewis lung carcinoma (LLC) subcutaneous xenograft model was established, and high-throughput transcriptome sequencing was performed. Combined treatment with GJXJD and a PD-1 inhibitor significantly suppressed tumor growth, achieving a tumor inhibition rate of 54.48 %, which was substantially higher than that of either monotherapy (GJXJD: 27.58 %; anti-PD-1: 25.76 %). Transcriptomic analysis indicated notable enrichment and downregulation of the PI3K/AKT signaling pathway. Immunohistochemistry revealed inhibition of the PI3K/AKT/NF-κB axis and subsequent downregulation of PD-L1 expression. GJXJD treatment increased spleen and thymus indices, reduced serum IL-17 levels by approximately 40 %, and promoted the secretion of IL-2, IFN-γ, and TNF-α by about 1.5- to 2-fold. Enhanced infiltration of CD3⁺, CD4⁺, and CD8⁺ T cells were observed in tumor tissues, with CD8⁺ T cell infiltration increasing by over 3-fold in the combination group. H&amp;E staining showed no significant pathological changes in major organs. These findings demonstrate that GJXJD potentiates the anti-tumor effect of PD-1 inhibitors by inhibiting the PI3K/AKT/NF-κB pathway, reducing PD-L1 expression, and ameliorating immune evasion. The identified active components provide a chemical basis for its efficacy, supporting GJXJD as a promising adjuvant agent for lung cancer immunotherapy.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107085"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition and evaluation of the antipsoriatic potential of Cistus sintenisii in mouse model","authors":"Dionysia Alvanou ,&nbsp;Ekaterina-Michaela Tomou ,&nbsp;Maria Anagnostou ,&nbsp;Christina Barda ,&nbsp;Anastasia-Ioanna Papantonaki ,&nbsp;Ioannis Sfiniadakis ,&nbsp;Jane Anastassopoulou ,&nbsp;Andreas Tzakos ,&nbsp;Michail Rallis ,&nbsp;Helen Skaltsa","doi":"10.1016/j.fitote.2026.107103","DOIUrl":"10.1016/j.fitote.2026.107103","url":null,"abstract":"<div><div><em>Cistus</em> species have been used in the treatment of various ailments, including skin disorders in the traditional medicine. The present study aimed to evaluate the antipsoriatic potential of <em>Cistus sintenisii</em> extracts, using an imiquimod-induced psoriasis mouse model. The most promising effects were observed in the highest concentrations of the extracts, which attenuated psoriatic alterations, such as hyperplasia of the epidermis and inflammation and improved epidermal structure. Furthermore, reduction of approximately 50% in the Psoriasis Area and Severity Index (PASI) score on day 6 indicated a favorable clinical response along with the histological outcomes. In addition, the chemical composition of the aqueous extract was studied, and nine compounds were isolated and identified, namely catechin, quercetin 3-O-α-L-rhamnoside, myricetin 3-O-α-L-rhamnoside, myricetin 3-O-α-L-arabinoside, myricetin 3-O-β-D-galactoside, gallic acid, shikimic acid, protocatechuic acid, and gentisic acid 5-O-β-D-xyloside. These findings may play a key role in the therapeutic potency of <em>C. sintenisii</em> against psoriasis, as well as support the traditional use of <em>Cistus</em> species in treating skin disorders.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107103"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}