Fitoterapia最新文献

{"title":"The aqueous extract of Atractylodes Macrocephalae Rhizoma attenuates sarcopenia caused by unhealthy dietary patterns and immobilization via the alleviation of atrophy of skeletal muscles in C57BL/6JNifdc.","authors":"Su Gao, Jie Su, Jingjing Yu, Meiqiu Yan, Yu Wang, Caozheng Shi, Hengpu Zhou, Yuzhong Du, Suhong Chen, Guiyuan Lv","doi":"10.1016/j.fitote.2025.106727","DOIUrl":"https://doi.org/10.1016/j.fitote.2025.106727","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of aqueous extract of Atractylodes atractylodes Rhizoma (AME) in improving muscle atrophy in mice, and to explore its mechanism of action.</p><p><strong>Methods: </strong>We induced skeletal muscle atrophy in mice by simulating human unhealthy lifestyle habits and concurrently administered them with AME. The polysaccharide content in the AME was determined using a UV-Vis spectrophotometer. The contents of three primary pharmacologically active components in the AME were quantified via HPLC. Body composition analysis was conducted weekly on the mice, and the mass of hindlimb skeletal muscles was measured after six weeks of treatment. Histopathological analysis of the skeletal muscles was performed using hematoxylin and eosin (HE) staining to evaluate the effect of AME on muscle atrophy. Mice grip strength and endurance were measured to assess their motor abilities and skeletal muscle function. Mechanisms underlying the ameliorative effect of AME on skeletal muscle atrophy were explored using qPCR and immunofluorescence staining techniques.</p><p><strong>Results: </strong>The modeling method employing alternate-day feeding with high-fat high-sugar diet combined with hindlimb immobilization significantly reduced the body weight of model mice within two weeks (P < 0.0001). Administration of Atractylodis Macrocephalae Radix aqueous extract to mice for six weeks increased body weight, total lean mass, and fat mass (P < 0.01, P < 0.001, P < 0.001 respectively). It also augmented the wet weight of the quadriceps femoris, gastrocnemius, soleus, and tibialis anterior muscles (P < 0.001, P < 0.01, P < 0.05, P < 0.05 respectively), enlarged the cross-sectional area of gastrocnemius muscle fibers, and improved the pathological morphology of skeletal muscles. Additionally, it enhanced skeletal muscle function in mice, increasing grip strength and endurance (P < 0.05), and augmented hindlimb microcirculatory blood flow (P < 0.01). Serum creatinine levels in mice were increased (P < 0.001), and the expression levels of genes related to skeletal muscle proliferation and differentiation, including Myf5, MyoD, Myogenin, Mef2, and myosin, were upregulated, Trim63 and Fbxo32 were downregulated. Furthermore, the conversion of type I to type II muscle fibers in the soleus muscle was improved.</p><p><strong>Conclusion: </strong>Long-term IF & HFHS dietary patterns combined with hind limb immobilization can decrease overall muscle mass in mice, leading to a transformation of slow muscle fibers to fast muscle fibers. AME can increase skeletal muscle mass, enhance muscle function, and resist the transformation of slow muscle fibers to fast muscle fibers by regulating the expression of myogenic markers.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106727"},"PeriodicalIF":2.5,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathophysiology and impact of testicular torsion/detorsion on male reproduction: Therapeutic approach with some phytochemicals","authors":"Deiaa E. Elsayed Abouzed ,&nbsp;Duaa Abdullah Bafail ,&nbsp;Shereen Mahmoud Refaie ,&nbsp;Mohamed S.A. Abdelkader ,&nbsp;Hend A.A. Ezelarab ,&nbsp;Moustafa O. Aboelez","doi":"10.1016/j.fitote.2025.106722","DOIUrl":"10.1016/j.fitote.2025.106722","url":null,"abstract":"<div><div>Testicular torsion is a typical urologic emergency that occurs when the spermatic cord twists and impairs blood flow to the testicles. It results in hypoxia, oxidative stress, inflammation, and ultimately fetal harm and apoptosis. It takes a pathologist's quick diagnosis and surgical action to save fetal function. Reperfusion following extended ischemia exacerbates inflammation and oxidative damage, making germ-cell apoptosis more difficult. Several complicated signaling pathways, including neutrophil activation, endothelial dysfunction, cytokine release, and stress-related gene expression, contribute to germ cell death, followed by subfertility or sterility. Many phytochemicals have shown promising potential in experimental models to counteract some pathophysiological mechanisms. This article reviews the complex pathophysiology caused by reflow, the resulting disruption of the hypothalamic-pituitary-gonadal axis, and the adverse effects on male fertility. The therapeutic implications of some bioactive phytochemicals as potential contributors to preserving testicular viability are also discussed to explore their roles. They determine the scope for well-designed clinical research. This may pave the way for combination therapy to improve outcomes and preserve fertility in cases of testicular torsion.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"185 ","pages":"Article 106722"},"PeriodicalIF":2.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition and anti-colitis efficacy analysis of ethanol and aqueous extraction of Moringa oleifera leaves","authors":"Yuemin Feng ,&nbsp;Junjie Huang ,&nbsp;Jingzhi Gong ,&nbsp;Weilong Peng ,&nbsp;Chenglong Yu ,&nbsp;Yuying Huai ,&nbsp;Chaoyang Chen ,&nbsp;Ruonan Bo ,&nbsp;Mingjiang Liu ,&nbsp;Jingui Li","doi":"10.1016/j.fitote.2025.106720","DOIUrl":"10.1016/j.fitote.2025.106720","url":null,"abstract":"<div><div><em>Moringa oleifera</em> (MO)is a perennial tropical deciduous plant, its leaves (MOL) have both medicinal and edible values. Ulcerative colitis (UC) is characterized by recurrent and nonspecific intestinal inflammatory disease, and developing new treatment is still urgently needed. This study focused on the main composition and anti-colitis efficacy of crude ethanol and aqueous extraction of MOL (EEMOL &amp; AEMOL). The results showed that EEMOL and AEMOL had similar contents and monosaccharide composition, while EEMOL had over three times more flavonoid content than AEMOL. Both EEMOL and AEMOL noticeably alleviated dextran sodium sulfate-induced murine UC by reducing the serious weight loss, disease activity index scores, colon shortening, and bloody diarrhea. EEMOL and AEMOL significantly inhibited the TLR4/NF-κB signaling pathway activation, reversed the expression of pro-inflammatory cytokines mRNA and anti-apoptotic proteins, while safeguarded the intestinal structure and tight junctions. In addition, MOL extract restored gut microbiota dysbiosis to varying degrees in the colitis model. Overall, above findings revealed the protective effect of EEMOL and AEMOL on UC is mainly achieved <em>via</em> inhibiting inflammation and apoptosis, maintaining the intestinal barrier, and reversing the microbiota dysbiosis. These data illustrated that both EEMOL and AEMOL have the potential to be an alternative to UC.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"185 ","pages":"Article 106720"},"PeriodicalIF":2.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue distribution of seven coptidis alkaloids in normal and insomnia rats affected by Cinnamomi cortex (Cinnamomum cassia) using liquid chromatography-tandem mass spectrometry.","authors":"Yunfang Huang, Qing Miao, Ning Chen, Tianyue Yan, Yanyi Li, Xiaobin Huang, Yujie Zhang","doi":"10.1016/j.fitote.2025.106723","DOIUrl":"https://doi.org/10.1016/j.fitote.2025.106723","url":null,"abstract":"<p><p>Jiao-Tai-Wan (JTW), composed of Coptidis Rhizoma (CR) and Cinnamomi Cortex (CC) in a 10:1 ratio (w/w), is an ancient prescription effectively used for treating insomnia in China. The pharmacodynamic components of JTW are primarily coptidis alkaloids from CR; however, the therapeutic mechanism remains unclear now. This study aimed to discuss the effects of CC on pharmacokinetic characteristics of CR by analyzing the tissue distribution of seven main coptidis alkaloids. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established to simultaneously quantify the contents of these seven coptidis alkaloids in tissues. The tissue distribution and pharmacokinetic profiles of these alkaloids were investigated in both normal and insomniac rats treated with CR alone or JTW. The LC-MS/MS method demonstrated high reproducibility and accuracy upon validation. Coptidis alkaloids were predominantly distributed in the heart, liver, and kidneys. Berberine was the most accumulated component in all tissues, while magnoflorine and berberrubine mainly accumulated in the liver and kidneys, respectively. There were significant differences in the distribution pharmacokinetic parameters of coptidis alkaloids between normal and insomniac rats (p < 0.05). The combination of CR and CC reduced the accumulation of coptidis alkaloids in normal rats and significantly increased it in insomniac rats (p < 0.05). These findings suggest that tissue distribution characteristics of coptidis alkaloids can be altered in a state of insomnia and after compatibility, the accumulation of alkaloids can be increased in vivo. This study reveals the pharmacokinetic interaction between CR and CC from the perspective of tissue distribution and provides valuable insights into the rationality of the combination of CR and CC and the mechanism of JTW in treating insomnia.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106723"},"PeriodicalIF":2.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights to phytochemistry, analytical methods, pharmacological activities, stability, and specifications of alantolactone.","authors":"Shimple Thakur, Anchal Bhatia, Rupali Kohal, Ghanshyam Das Gupta, Sant Kumar Verma","doi":"10.1016/j.fitote.2025.106716","DOIUrl":"https://doi.org/10.1016/j.fitote.2025.106716","url":null,"abstract":"<p><p>Alantolactone, a sesquiterpene lactone derived from plants of the Asteraceae family, has been extensively studied for its diverse pharmacological potential. Research suggests that various biochemical pathways are involved in the bioactivity of alantolactone, such as STAT3 (Single transducer and activator of transcription 3), MAPK/NF-κB (Mitogen-activated protein kinases/ Nuclear factor kappa-light chain enchancerof B cells), and YAP1/TAZ (Yes-associated protein 1/Transcriptional co-activator with PDZ-binding motif pathway) pathways for anticancer, proinflammatory cytokines NF-ΚB and STAT3 signaling for anti-inflammatory, Nrf2 (Nuclear factor erythroid 2-related factor 2) pathway for antioxidant, L-type voltage-gated calcium channels for antihypertensive, enhanced reactive oxygen species (ROS) production for antifungal properties, and potential to alter lipid metabolism via suppression of IL-6 (Interleukin-6) stimulated TLR-4 (Toll-like receptor) expression. Additionally, alantolactone has been explored for its insecticidal, antibacterial, and antiviral properties. The review provides a comprehension on phytochemistry, including chemical framework, biosynthesis, techniques for isolating and extracting alantolactone, synthetic transformations, analytical aspects, a detailed understanding of the therapeutic potential established through in vivo and in vitro studies, bioavailability and bio-accessibility, toxicity profile, and the patent landscape on alantolactone.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106716"},"PeriodicalIF":2.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of anti-inflammatory pyrrole alkaloids from Curvularia lunata CL1","authors":"Qianqian Xu ,&nbsp;Yuben Qiao ,&nbsp;Chong Dai ,&nbsp;Liangfang Pang ,&nbsp;Liangxia Li ,&nbsp;Yuanyuan Lu","doi":"10.1016/j.fitote.2025.106718","DOIUrl":"10.1016/j.fitote.2025.106718","url":null,"abstract":"<div><div>Two novel pyrrole alkaloids, pyrinoids A (<strong>1</strong>) and B (<strong>2</strong>), were isolated from the culture of <em>Curvularia lunata</em> CL1. Their structures were elucidated using extensive spectroscopic methods, including NMR, HRESIMS, IR, and UV, alongside electronic circular dichroism (ECD) analysis for absolute configuration determination. Both compounds exhibited significant inhibitory activity against lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW264.7 cells, with IC₅₀ values of 14.06 ± 0.74 and 12.64 ± 3.82 μM, respectively. Additionally, pyrinoids A and B enhanced the secretion of the anti-inflammatory cytokine IL-10 and suppressed pro-inflammatory mediators IL-6 and PGE2. They also modulated the transcription levels of key inflammatory markers, downregulating IL-6 and iNOS while upregulating IL-10 expression. These findings suggest that pyrinoids A and B possess promising potential as anti-inflammatory agents for mitigating inflammation in macrophage-mediated pathways.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"185 ","pages":"Article 106718"},"PeriodicalIF":2.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Megastigmane sesquiterpenoids from the pericarps of Trichosanthes kirilowii and their anti-complementary and anti-inflammatory activities","authors":"Ji-Bin Zou ,&nbsp;Jia-Ren Lyu ,&nbsp;Leverne Wang ,&nbsp;Shui-Yuan Yang ,&nbsp;Yan Lu ,&nbsp;Dao-Feng Chen","doi":"10.1016/j.fitote.2025.106715","DOIUrl":"10.1016/j.fitote.2025.106715","url":null,"abstract":"<div><div>The pericarps of <em>Trichosanthes kirilowii</em> Maxim. <em>(T. kirilowi)</em> have been traditionally used in Chinese medicine for their heat-clearing properties. This study employed anti-complementary activity-guided fractionation and purification to isolate eleven compounds from the pericarps of <em>T. kirilowi</em>, including two new megastigmane sesquiterpenoids (<strong>6, 8</strong>), seven previously identified megastigmane sesquiterpenoids (<strong>1–5, 7, 9</strong>), and two known flavonoids (<strong>10,11</strong>). The structures were elucidated using spectroscopic techniques (NMR, HRESIMS, IR, and UV) and computational chemistry methods with CD spectroscopy. Biological evaluation demonstrated that four megastigmane sesquiterpenoids (<strong>3, 4, 5, 6</strong>) and two flavonoids (<strong>10, 11</strong>) exhibited both anti-complementary activities (CH₅₀ values ranging from 0.906 ± 0.075 mM to 4.933 ± 0.318 mM) and anti-inflammatory activities (inhibition rates: 14.30 ± 4.234 % to 72.87 ± 1.594 %). Furthermore, the new megastigmane sesquiterpenoid (<strong>6</strong>), featuring a 3-hydroxy-3-methylglutaryl (HMG) substituent, and the flavonoid glucoside (<strong>11</strong>) modulated cytokine levels in LPS-stimulated ANA-1 cells by reducing IL-6 and MCP-1 while increasing IL-10.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"185 ","pages":"Article 106715"},"PeriodicalIF":2.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the antihypertensive activity of the aqueous extract and the major flavonoid from Boldoa purpurascens Cav. leaves","authors":"Dulce María González Mosquera ,&nbsp;Yudith Cañizares Carmenate ,&nbsp;Amanda Ivet Rivero Arbolay ,&nbsp;Ramón Romero Borges ,&nbsp;María Luisa García Gómez ,&nbsp;Freisman Blanco Machado ,&nbsp;Kenia González Valcarel ,&nbsp;Roberto Díaz-Amador ,&nbsp;Anayancy Osorio-Madrazo ,&nbsp;Gilberto Cairo Saez ,&nbsp;Emmy Tuenter","doi":"10.1016/j.fitote.2025.106724","DOIUrl":"10.1016/j.fitote.2025.106724","url":null,"abstract":"<div><div><em>Boldoa purpurascens</em> Cav., traditionally used in Cuba for hypertension, lacks ethnopharmacological validation. This study evaluated its aqueous extract and major flavonoid (4,5-dihydroxy-6,7-methylenedioxyflavonol-3-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-xylopyranoside) for antihypertensive effects in a salt-induced hypertensive rat model. Sprague Dawley rats (<em>n</em> = 34) were divided into six groups: normotensive control, hypertensive untreated, hypertensive + enalapril (15 mg/kg/day), hypertensive + <em>B. purpurascens</em> extract (40 or 80 mg/kg/day), and hypertensive + flavonoid (3 mg/kg/day). Blood pressure was measured pre- and post-treatment, while a histopathological analysis assessed the impact on kidney, heart, and brain tissues. It was found that the extract (80 mg/kg/day) significantly reduced systolic (−8.0 mmHg) and diastolic (−5.2 mmHg) blood pressures, comparable to control (enalapril). The flavonoid showed milder effects (−0.7 mmHg systolic, −2.5 mmHg diastolic). Pathological findings revealed reduced myocardial hypertrophy and reversible renal tubular damage with flavonoid treatment, while brain edema and congestion were absent. It was concluded that <em>B. purpurascens</em> exhibits dose-dependent antihypertensive activity, likely mediated by flavonoids. The major flavonoid attenuated organ damage, suggesting therapeutic potential. Future studies should assess the presence of other bioactive compounds in the <em>B. purpurascens</em> extract, as well as clarify the mechanism of action.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"185 ","pages":"Article 106724"},"PeriodicalIF":2.5,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanism of TLR4/NF-κB signaling pathway in hypoxic myocardial injury: Implications for traditional Chinese medicine therapy","authors":"Keke Liang ,&nbsp;Liang Xu ,&nbsp;Yanling Ta ,&nbsp;Renjie Wang ,&nbsp;Shuhe Ma ,&nbsp;Chenrong Xiao ,&nbsp;Xianxie Zhang ,&nbsp;Yue Gao ,&nbsp;Maoxing Li","doi":"10.1016/j.fitote.2025.106721","DOIUrl":"10.1016/j.fitote.2025.106721","url":null,"abstract":"<div><div>Hypoxic myocardial injury is the core mechanism of many cardiovascular diseases and poses a serious threat to global public health. Its pathogenesis involves energy metabolism disorder, oxidative stress, inflammatory response, apoptosis regulation imbalance, and other links, resulting in myocardial dysfunction and damage. In recent years, a large number of studies have confirmed that the Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling network can regulate hypoxic myocardial injury by mediating HMGB1, MyD88, Caspase-3, HIF-1α, TNF-α, NLRP3, HSP70, etc. Traditional Chinese Medicine (TCM) and its active components have obvious advantages in the treatment of complex diseases such as hypoxic myocardial injury, and inhibiting the key target of TLR4/NF-κB signaling network is one of the mechanisms of myocardial protection. However, there are few systematic reviews and summaries in this field. Based on this, this review summarizes the regulatory mechanism of TLR4/NF-κB signaling pathway involved in hypoxic myocardial injury and the intervention effect of TCM in recent years, to provide a theoretical basis for basic research and new drug development of hypoxic myocardial injury.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"185 ","pages":"Article 106721"},"PeriodicalIF":2.5,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Terpenoids from Euphorbia wallichii and their anti-inflammatory therapeutic potential","authors":"Qin Yang ,&nbsp;Yali Wang ,&nbsp;Zhiqi Zhang ,&nbsp;Ziyan Gao ,&nbsp;Qianhui Liu ,&nbsp;Hua Li ,&nbsp;Lixia Chen","doi":"10.1016/j.fitote.2025.106725","DOIUrl":"10.1016/j.fitote.2025.106725","url":null,"abstract":"<div><div>Twelve known compounds and four undescribed terpenoids, including wallbiaoid B (<strong>1</strong>), wallingen A (<strong>8</strong>), wallabielide B (<strong>13</strong>) and wallabiaid A (<strong>14</strong>) were isolated from the plant <em>Euphorbia wallichii</em>. The structure and stereochemistry of these compounds were evaluated by 1D and 2D NMR, calculated electronic circular dichroism (ECD) and mass spectrometry analysis. In addition, the inhibitory effect of all these isolated compounds on LPS-induced nitric oxide (NO) overproduction in mouse monocyte-macrophage leukemia cell RAW264.7 was determined. Representative compound <strong>10</strong> was molecularly docked with ALB protein to predict its efficacy against inflammation.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"185 ","pages":"Article 106725"},"PeriodicalIF":2.5,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}