Study on the therapeutic effect of Fallopia aubertii (L.Henry) Holub against HUA combined with AGA and the separation of chemical components of ethyl acetate extract.

Abstract

Fallopia aubertii (L.Henry) Holub, also known as Polygonum aubertii, is a traditional Tibetan medicinal plant widely used for its pharmacological effects, including clearing heat, dispelling wind and dampness, and promoting diuresis.Modern pharmacological studies have shown that Fallopia aubertii has better pharmacological effects on analgesic, anti-inflammatory and anti-hyperuricemia. However, its mechanism of action has not been elucidated.Therefore, in this study, the therapeutic effects of different extracts of Fallopia aubertii on HUA + AGA model rats were verified by in vivo experiments. Meanwhile, the main active parts of Fallopia aubertii were enriched and purified by on-line MCI column combined with two-dimensional preparative liquid chromatography to prepare high-purity single compounds, which initially explored the pharmacological constituents and mechanism of action of Fallopia aubertii. The pharmacological experiments showed that the ethyl acetate extracted part of Fallopia aubertii had significant efficacy in the treatment of acute gouty arthritis combined with hyperuricemia in rats. In addition, the main compounds isolated from the Fr6 fraction of the ethyl acetate fraction of Fallopia aubertii were flavonoids. It is speculated that the mechanism of action may be that the flavonoid compounds in Fallopia aubertii ethyl acetate inhibit the activity of XOD and reduce the level of inflammatory factors, thereby slowing down the synthesis of uric acid and alleviating inflammation. The online MCI column combined with two-dimensional liquid chromatography used in this study showed great potential in the separation of high-purity compounds from Fallopia aubertii, providing broad application prospects for the efficient separation of single compounds in natural plants.