FitoterapiaPub Date : 2024-11-14DOI: 10.1016/j.fitote.2024.106299
Priscilla O. Aiyedun , Mubo A. Sonibare , Badara Gueye , Dirk C. Albach , Julia Heil , Gertrud E. Morlock
{"title":"Antidiabetic and antioxidant profiling of 67 African trifoliate yam accessions by planar on-surface assays versus in vitro assays","authors":"Priscilla O. Aiyedun , Mubo A. Sonibare , Badara Gueye , Dirk C. Albach , Julia Heil , Gertrud E. Morlock","doi":"10.1016/j.fitote.2024.106299","DOIUrl":"10.1016/j.fitote.2024.106299","url":null,"abstract":"<div><div>Trifoliate yam (<em>Dioscorea dumetorum</em>) is traditionally used to treat diabetics in Nigeria. However, almost no information is available on its antidiabetic constituents and their natural variance. Hence, the activity of methanolic tuber extracts of 67 trifoliate yam accessions from the largest collection in Africa was proven by four colorimetric antidiabetic and antioxidant <em>in vitro</em> assays, as diabetes is also linked with oxidative stress. For the first time, selected accessions were also analyzed by planar bioactivity profiling. It has a comparatively higher, more differentiated information content, is more sustainable in terms of material consumption, and enables straightforward compound prioritization and characterization. Up to a dozen individual antioxidant zones were revealed as well as one prominent zone inhibiting α-glucosidase and α-amylase. The latter inhibition zone was tentatively assigned to palmitic, linoleic, oleic, linolenic, oxo-nonanoic fatty acids by direct elution to heated electrospray ionization high-resolution mass spectrometry.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"180 ","pages":"Article 106299"},"PeriodicalIF":2.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
FitoterapiaPub Date : 2024-11-13DOI: 10.1016/j.fitote.2024.106296
Mingliang Zhang , Mengrong Niu , Jiangping Fan , Zihan Lu , Zhixiang Zhu , Bowen Gao , She-Po Shi
{"title":"Synthesis and pharmacological activity evaluation of 2-(2-Phenylethyl)chromone analogues: The principal components in agarwood","authors":"Mingliang Zhang , Mengrong Niu , Jiangping Fan , Zihan Lu , Zhixiang Zhu , Bowen Gao , She-Po Shi","doi":"10.1016/j.fitote.2024.106296","DOIUrl":"10.1016/j.fitote.2024.106296","url":null,"abstract":"<div><div>2-(2-Phenylethyl)chromones (PECs), the pivotal constituents responsible for the distinctive aroma and pharmacological potential of agarwood, are primarily obtained through extraction from natural materials. The restricted availability of agarwood has, however, hindered a comprehensive and systematic evaluation of their biological properties. In this study, we have chemically synthesized a total of 38 PEC derivatives, including 23 new compounds that had not been previously isolated from agarwood. These compounds were then evaluated for their inhibitory effects on nitric oxide release, neutrophil superoxide production, as well as their activities against tyrosinase, acetylcholinesterase, and <em>α</em>-glucosidase. The preliminary screening resulted in the identification of promising leads with potential therapeutic activities, and the structure-activity relationship of the synthesized PECs have also been briefly discussed. The results provide a foundation for the synthesis and pharmacological exploration of PECs, which sheds light on the pharmacological potential of agarwood's secondary metabolites.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"180 ","pages":"Article 106296"},"PeriodicalIF":2.5,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chemical profiles and antibacterial actions of Zanthoxylum acanthopodium DC. Essential oil growing in Indonesia","authors":"Abdi Wira Septama , Eldiza Puji Rahmi , Aprilia Nur Tasfiyati , Nur Aini Khairunnisa , Halimah Raina Nasution , Nilesh Nirmal , Sofna Dewita Sari Banjarnahor , Nurhadi , Dadang Priyatmojo","doi":"10.1016/j.fitote.2024.106300","DOIUrl":"10.1016/j.fitote.2024.106300","url":null,"abstract":"<div><div>Foodborne illness caused by pathogens has been a major health problem. Antibacterial resistance renders therapeutic options for treating the condition more limited, hence there is a growing interest in finding novel antibacterial alternatives. <em>Zanthoxylum acanthopodium</em> (Rutaceae) essential oil (ZAEO) has a substantial potential to suppress the growth of bacterial infections. This study was conducted to evaluate antibacterial activity of ZAEO against four species of foodborne pathogens that cause potential infections. The checkerboard assay was used to identify synergistic effect of ZAEO and tetracycline, while bacteriolysis test was applied to evaluate the ability of ZAEO releasing material genetics of bacteria, inhibiting biofilm formation, and suppressing efflux pump. The oils of <em>Z. acanthopodium</em> was evaluated and showed that the highest constituents were limonene and geranyl acetate at 47.13 % and 23.84 %, respectively. These oils have the strongest ability to inhibit <em>Escherichia coli</em> compared to other pathogens with MIC value of 62.5 μg/mL. ZAEO and tetracycline also presented a synergistic effect against <em>E. coli</em> with FICI value of 0.37. Moreover, this combination of ZAEO and antibiotic shown as potential antibacterial agent by several mechanisms such as, decreasing biofilm formation, releasing ions, rupturing membrane cells, and suppressing the efflux pump of <em>E. coli.</em> It can be concluded that ZAEO and tetracycline presented a promising antibacterial activity, which can be explored further to develops antibacterial agents against foodborne pathogens.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"180 ","pages":"Article 106300"},"PeriodicalIF":2.5,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
FitoterapiaPub Date : 2024-11-11DOI: 10.1016/j.fitote.2024.106274
Hongliu Xie, Fang Lin, Fei Shi, Elaine Johnstone, Yaqi Wang, Yang An, Jun Su, Jiayin Liu, Qinghai Dong, Jihua Liu
{"title":"Synthesis, biological evaluation and mechanism study based on network pharmacology of amino acids esters of 20(S)-protopanaxadiol as novel anticancer agents.","authors":"Hongliu Xie, Fang Lin, Fei Shi, Elaine Johnstone, Yaqi Wang, Yang An, Jun Su, Jiayin Liu, Qinghai Dong, Jihua Liu","doi":"10.1016/j.fitote.2024.106274","DOIUrl":"https://doi.org/10.1016/j.fitote.2024.106274","url":null,"abstract":"<p><p>As one of the metabolites of ginseng, 20(S)-protopanaxadiol (PPD) is a compound with dammarane-type tetracyclic triterpene, which performs a wide range of anticancer activities. In this study, PPD was used as a lead. A series of compounds were synthesized respectively with 11 amino acids through esterification and were evaluated for their cytotoxicity against several cancer cell lines. One of the synthetic products (PL) exhibited potent inhibitory effect on Huh-7 cells relative to that of PPD in vitro. Subsequently, the Annexin V-FITC /PI staining assay was used to verify that PL induced apoptosis of Huh-7 cells in a dose-dependent manner. A UPLC-Q/TOF-MS analysis method was established and validated for assessing pharmacokinetic properties after the administration of PPD and PL in rats. The results showed that compared with PPD, T<sub>1/2</sub>of PL in rats was prolonged, and the peak time was delayed, resulting in broader tissue distribution of the compound in the body. In addition, the targets of PL against several cancers were predicted and analyzed via network pharmacology. Molecular docking simulations demonstrated that PL interacted with the active sites of the above targets. In conclusion, this study provided a theoretical basis for the development and clinical application of anti-tumor activity of PPD.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106274"},"PeriodicalIF":2.5,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of therapeutic and cosmetic cream based on flavonoids","authors":"S.M. Adekenov, D.L. Savchenko, A.O. Kenzhebekov, A.N. Zhabaeva, A.S. Adekenova, V.V. Polyakov","doi":"10.1016/j.fitote.2024.106283","DOIUrl":"10.1016/j.fitote.2024.106283","url":null,"abstract":"<div><div>The article discusses the results of the development of a cream based on <em>Populus balsamifera</em> L. bud flavonoids, which have anti-inflammatory, skin-regenerating effects.</div><div>A cream's anti-inflammatory and wound-healing activity based on a flavonoid substance has been studied. In an experiment on a model of an cut skin wound, it was determined that a cream based on the sum of flavonoids of <em>Populus balsamifera</em> L. buds, containing pinostrobin, pinocembrin, tectochrysin, exhibits anti-inflammatory and wound-healing activity. Considering that the effectiveness of local wound treatment mainly depends on the rational treatment of the first phase of the wound process, we have developed a 1 % flavonoid cream and its production technology.</div><div>The technology for the production of flavonoid cream includes extraction and isolation of the sum of flavonoids from the raw material of <em>Populus balsamifera</em> L. buds, preparation of flavonoid substance and excipients, preparation of the cream base and cream, packaging and labelling of cream. Based on the results of our experiments, a pilot industrial regulation for the production of a new cream based on the sum of flavonoids of <em>Populus balsamifera</em> L. buds has been developed, and pilot production of an original therapeutic and cosmetic cream was organised.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"180 ","pages":"Article 106283"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
FitoterapiaPub Date : 2024-11-01DOI: 10.1016/j.fitote.2024.106282
Idris Adewale Ahmed , Nor Hisam Zamakshshari , Maryam Abimbola Mikail , Ibrahim Bello , Md. Sanower Hossain
{"title":"Garcinia flavonoids for healthy aging: Anti-senescence mechanisms and cosmeceutical applications in skin care","authors":"Idris Adewale Ahmed , Nor Hisam Zamakshshari , Maryam Abimbola Mikail , Ibrahim Bello , Md. Sanower Hossain","doi":"10.1016/j.fitote.2024.106282","DOIUrl":"10.1016/j.fitote.2024.106282","url":null,"abstract":"<div><div>Cellular senescence, the irreversible arrest of cell division, is a hallmark of aging and a key contributor to age-related disorders. Targeting senescent cells represents a promising therapeutic approach to combat these ailments. This review explores the potential of <em>Garcinia</em> species, a genus rich in flavonoids with established antioxidant and anti-inflammatory properties, as a source of natural anti-senescence agents. We investigate the intricate connections between aging, cellular senescence, and oxidative stress, highlighting the detrimental effects of free radicals on cellular health. Furthermore, we analyze the diverse array of flavonoids identified within <em>Garcinia</em> and their established cellular mechanisms. We critically evaluate the emerging evidence for the anti-senescence potential of flavonoids in general and the limited research on <em>Garcinia</em> flavonoids in this context. By identifying existing knowledge gaps and paving the way for future research, this review underscores the exciting potential of <em>Garcinia</em> flavonoids as natural anti-senescence agents. These agents hold promise for not only promoting healthy aging but also for the development of cosmeceutical products that combat the visible signs of aging.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"180 ","pages":"Article 106282"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
FitoterapiaPub Date : 2024-10-29DOI: 10.1016/j.fitote.2024.106279
Prateek Singh Bora, Shivani Puri, Prithvi Pal Singh, Upendra Sharma
{"title":"Biochemometric-guided isolation of new Isosteroidal alkaloids from Fritillaria cirrhosa D.Don (Liliaceae, syn. Fritillaria roylei Hook) as acetylcholinesterase inhibitors.","authors":"Prateek Singh Bora, Shivani Puri, Prithvi Pal Singh, Upendra Sharma","doi":"10.1016/j.fitote.2024.106279","DOIUrl":"10.1016/j.fitote.2024.106279","url":null,"abstract":"<p><p>Globally, Alzheimer's disease is an urgent public health concern with the ageing population in developing nations. Recent studies have identified isosteroidal alkaloids as promising therapeutic agents for Alzheimer's treatment. Fritillaria species are well-known rich sources of steroidal and isosteroidal alkaloids. In this context, the current study focuses on the biochemometric-guided isolation of three previously undescribed and two known isosteroidal alkaloids as acetylcholinesterase (AChE) inhibitors from the bulbs of Fritillaria cirrhosa D.Don. The isolated molecules were characterized by NMR, HR-ESI-MS, FT-IR, and DP4+ analysis. Subsequently, all isolates were evaluated for AChE inhibitory activity using Ellman's method. Among the evaluated molecules, 1 (IC<sub>50</sub>: 33.0 ± 4.4 μM) and 5 (IC<sub>50</sub>: 24.7 ± 4.5 μM) showed promising AChE inhibition in vitro. Enzyme kinetic studies of isolated molecules revealed mixed inhibition kinetics with K<sub>i</sub> varying from 1.3 to 24.4 μM. Moreover, the in silico studies showed excellent binding affinities of isolated molecules with the target protein and good drug-like ADMET properties. The present study identified new isosteroidal alkaloids as promising AChE inhibitors from F. cirrhosa bulbs via a biochemometric approach and advocated their further exploration for treating neurodegenerative disorders.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106279"},"PeriodicalIF":2.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
FitoterapiaPub Date : 2024-10-28DOI: 10.1016/j.fitote.2024.106281
Wei-Wei Fan , Dan Yang , Zhong-Quan Cheng , Liu Yang , Hui-Yan Shao , Xiao-Nian Li , Feng-Qing Xu , Jiang-Miao Hu
{"title":"Isosativene and sativene sesquiterpene derivatives from Dendrobium nobile","authors":"Wei-Wei Fan , Dan Yang , Zhong-Quan Cheng , Liu Yang , Hui-Yan Shao , Xiao-Nian Li , Feng-Qing Xu , Jiang-Miao Hu","doi":"10.1016/j.fitote.2024.106281","DOIUrl":"10.1016/j.fitote.2024.106281","url":null,"abstract":"<div><div>Two unusual isosativene sesquiterpene derivatives, named dendronobilol A (<strong>1</strong>) and dendronobilside A (<strong>2</strong>), and two unusual sativene sesquiterpene derivatives, named dendronobilsides B (<strong>3</strong>) and C (<strong>4</strong>), had been isolated from the stems of <em>Dendrobium nobile</em>. The structures of all the compounds were established using spectroscopic methods and by comparison with literature data, and their absolute configurations were confirmed via single-crystal X-ray diffraction data and electronic circular dichroism (ECD) calculations. Dendronobilol A (<strong>1</strong>) and dendronobilside A (<strong>2</strong>) possessed a unique tricyclo[4.3.0.1<sup>2, 8</sup>]decan ring system, while dendronobilsides B (<strong>3</strong>) and C (<strong>4</strong>) presented a unique tricyclo[4.4.0.0<sup>2, 8</sup>]decan core carbon skeleton. The above two types of sesquiterpene derivatives had been isolated and purified from plants for the first time. Compounds <strong>1</strong> and <strong>2</strong> exhibited significant effects on glucose consumption with doses of 20 and 40 μmol/L in insulin-resistant HepG2 cells, and thus improve insulin resistance.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"179 ","pages":"Article 106281"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Polygala tenuifolia root extract attenuates ischemic stroke by inhibiting HMGB1 trigger neuroinflammation","authors":"Pingping Shen , Libang Zhang , Xuewa Jiang , Richa Raj , Boyang Yu , Jian Zhang","doi":"10.1016/j.fitote.2024.106280","DOIUrl":"10.1016/j.fitote.2024.106280","url":null,"abstract":"<div><div><em>Polygala tenuifolia</em> Willd., a famous traditional Chinese medicine, has been widely applied to treat central nervous system diseases. In this study, <em>P. tenuifolia</em> root extract exhibited a moderate anti-ischemic effect on <em>in-vitro</em> oxygen-glucose deprivation/reperfusion (OGD/R) model. In transient middle cerebral artery occlusion (tMCAO) rats, <em>P. tenuifolia</em> root extract significantly attenuated brain infarction and neurological deficits in a dose-dependent manner. Compared with the sham group, the release of damage-associated molecular patterns (DAMPs)-HMGB1 in the ischemic brain was significantly higher, which was inhibited by <em>P. tenuifolia</em> root extract. To further explore such neuroprotective effects whether associated with aseptic inflammation, HMGB1-activated BV2 microglial cells model was established. The extract of <em>P. tenuifolia</em> was found to inhibit the downstream inflammatory response driven by HMGB1, with an IC<sub>50</sub> value of 49.46 μg/mL. In addition, the extract was also found to be able to directly interact with HMGB1 in the surface plasmon resonance (SPR) experiment. Phytochemical studies showed that the extract of <em>P. tenuifolia</em> root contains a large number of terpenoids, oligosaccharides and phenolic compounds, which likely contribute to the above observed biological activities. Our results not only provide some data support for the clinical application of <em>P. tenuifolia</em> against cerebral ischemia, but also clarify the potential target of <em>P tenuifolia</em>'s anti-inflammatory properties.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"180 ","pages":"Article 106280"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
FitoterapiaPub Date : 2024-10-28DOI: 10.1016/j.fitote.2024.106278
Guo Xu , Penghong Xu , Nan Wang , Weimin Qi , Yuxuan Pu , Nannan Kang , Jianlin Chu , Bingfang He
{"title":"Rare crocins ameliorate thrombus in zebrafish larvae by regulating lipid accumulation and clotting factors","authors":"Guo Xu , Penghong Xu , Nan Wang , Weimin Qi , Yuxuan Pu , Nannan Kang , Jianlin Chu , Bingfang He","doi":"10.1016/j.fitote.2024.106278","DOIUrl":"10.1016/j.fitote.2024.106278","url":null,"abstract":"<div><div>Crocin-4 is a water-soluble carotenoid that exhibits cardiovascular protection effects through anti-inflammatory and antioxidant effects. However, the pharmacodynamic effects and mechanisms of its analogues crocin-1 and crocin-2′ have not been reported. In this study, we evaluated the protective effects of rare crocins on cardiovascular systems. In ox-LDL induced HUVECs model, 0.02, 0.1, 0.5, 1, 2, 3, 4, 5, 6 μg/mL crocin-1 and crocin-2′ can increase cell viability by up to 80 %. Meanwhile, rare crocins at concentrations between 25–100 μg/mL crocin-1 and crocin-2′ reduced the lipid accumulation by 30 % in cholesterol-induced zebrafish larvae. What's more, the therapeutic potential of rare crocins on thrombosis has also been explored. In vitro experiments, rare crocin-1 and crocin-2′ at concentrations of 0.02, 0.05, 0.2, 0.5, 1, 2, 5, 10 μg/mL protected Human Umbilical Vein Endothelial Cells (HUVECs) against lipopolysaccharides-induced oxidative stress and inflammation. In vivo studies revealed that rare crocins at concentrations of 25, 50, 100, 150, 200, and 300 μg/mL exerted significant antithrombotic effect on arachidonic acid (AA)-induced zebrafish and there was nearly no potential risk for the deformity of zebrafish at 300 μg/mL dosages. In brief, rare crocins was viewed as a potentially useful candidate for the treatment of cardiovascular diseases because of its anti-inflammatory, antioxidant, and anticoagulant properties.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"179 ","pages":"Article 106278"},"PeriodicalIF":2.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}