Fitoterapia最新文献

{"title":"New dibenzofurans from Phellinus ribis and their anti-inflammatory activity","authors":"Ying Wang,&nbsp;Xiangyun Guo,&nbsp;Xinya Wang,&nbsp;Yuan Lu,&nbsp;Yiqun Li,&nbsp;Yongqing Zhang,&nbsp;Zhongying Fang,&nbsp;Ran Yang","doi":"10.1016/j.fitote.2026.107333","DOIUrl":"10.1016/j.fitote.2026.107333","url":null,"abstract":"<div><div>Four previously undescribed dibenzofurans and derivatives, named ribisin G–J (<strong>1</strong>–<strong>4</strong>), along with seven known compounds (<strong>5</strong>–<strong>11</strong>) were isolated from <em>Phellinus ribis</em>. Among them, compounds <strong>1</strong>–<strong>3</strong> were identified as fully aromatic dibenzofurans, whereas compound <strong>4</strong> was a dibenzofuran derivative. Their structures were elucidated by comprehensive spectroscopic analysis. The absolute configuration of compound <strong>4</strong> was further confirmed by ECD calculation. The anti-inflammatory activity of all compounds was assessed in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Notably, compound <strong>4</strong> exhibited inhibitory activity against nitric oxide (NO) production, with an IC₅₀ value of 12.23 ± 0.81 <em>μ</em>mol·L⁻¹. Further studies revealed that compound <strong>4</strong> significantly inhibited the expression of IL-6, IL-1<em>β</em> and TNF-<em>α</em>.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"192 ","pages":"Article 107333"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148257769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “ZhuYang TongBi decoction ameliorates pulmonary fibrosis by degrading TGFBR via CAV1” [Fitoterapia 192 (2026) 107300]","authors":"Jing Wang,&nbsp;Gege Liu,&nbsp;Ying Zhang,&nbsp;Zeyu Wang,&nbsp;Lin Tian,&nbsp;Tan Wang","doi":"10.1016/j.fitote.2026.107318","DOIUrl":"10.1016/j.fitote.2026.107318","url":null,"abstract":"","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"192 ","pages":"Article 107318"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148162341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taming the thunder god vine: Antiviral terpenoids from Tripterygium wilfordii","authors":"Wei Hong,&nbsp;Po Hu,&nbsp;Yingjie Qing","doi":"10.1016/j.fitote.2026.107330","DOIUrl":"10.1016/j.fitote.2026.107330","url":null,"abstract":"<div><div><em>Tripterygium wilfordii</em> (commonly known as thunder god vine), a perennial woody vine of the Celastraceae family predominantly distributed in southern China, has held a significant place in traditional Chinese medicine (TCM) for centuries. Its roots and leaves are rich repositories of diverse bioactive compounds, including the well-known Triptolide and Celastrol. Among these constituents, terpenoids stand out as a major and structurally complex class of phytochemicals that are fundamental to the plant's documented medicinal properties. Terpenoids have demonstrated particularly potent and unique antiviral effects against a range of specific viruses, positioning them as a highly promising class for antiviral drug discovery. Their inherent structural diversity presents significant opportunities for targeted modifications aimed at optimizing pharmacological profiles, such as enhancing potency and reducing potential toxicity. This review systematically summarizes the traditional applications and chemical profile of <em>Tripterygium wilfordii</em>, with a specific focus on the structural classification and antiviral mechanisms of its constituent terpenoids. We will detail their efficacy against specific viral targets, elucidate the underlying molecular mechanisms of action, and highlight recent advances in structural modification strategies. Ultimately, this synthesis seeks to provide a robust theoretical foundation for the rational development of <em>Tripterygium wilfordii</em>-derived terpenoids as novel antiviral agents.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"192 ","pages":"Article 107330"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148210586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into the inhibition of recombinant hepatitis E virus papain-like cysteine protease by Khaya grandifoliola hydro-Ethanolic extract: UHPLC-MS profiling, enzyme kinetics, computational modeling, and cell-based assays","authors":"Arnaud Fondjo Kouam,&nbsp;Cromwel Tepap Zemnou,&nbsp;Armel Jackson Seukep,&nbsp;Babayemi Olawale Oladejo,&nbsp;Brice Fredy Nemg Simo,&nbsp;Kerinyuy Juliene Kongnyuy,&nbsp;Armel Gaelle Kwesseu Fepa,&nbsp;Elisabeth Menkem Zeuko'o,&nbsp;Frédéric Nico Njayou,&nbsp;Paul Fewou Moundipa","doi":"10.1016/j.fitote.2026.107335","DOIUrl":"10.1016/j.fitote.2026.107335","url":null,"abstract":"<div><div>Hepatitis E virus (HEV), a significant cause of liver diseases, lacks specific treatments. <em>Khaya grandifoliola</em> used in traditional medicine for liver ailments, shows antiviral potential. While known to act against hepatitis C, its effect on HEV is unclear. This study investigates its hydro-ethanolic extract as a potential HEV inhibitor, targeting the HEV papain-like cysteine protease (HEV-PLpro). KHE's phytochemical profiling was determined using Waters-Acquity UHPLC-MS system. Recombinant HEV-PLpro, expressed in baculovirus-infected insect cells and purified via nickel-affinity and size-exclusion chromatography, was used to screen KHE's antiviral activity. The IC₅₀ and inhibition mechanism were determined using the fluorogenic substrate <em>Z</em>-RLRGG-AMC. Molecular docking and dynamics simulations predicted interactions and analyzed the stability of KHE's compounds with HEV-ORF1 (PDB ID: <span><span>6NU9</span><svg><path></path></svg></span>). HEV replication inhibition and cytotoxicity were evaluated in Huh7.5 cells using a Gaussia luciferase reporter system and MTT assay, respectively. Eighteen compounds comprising flavonoid-O-glycosides, phenolic and terpene glycoside were successfully identified. KHE exhibited mixed-type inhibition of HEV-PLpro proteolytic activity, with an IC₅₀ of 20.86 μg/mL, comparable to ribavirin (19.13 μg/mL), acting as competitive inhibitor. Notably, quercetin-3-[rhamnosyl-(1 → 2)-α-L-arabinopyranoside] and quercitrin exhibited stronger binding affinities (−7.68 and − 7.43 kcal/mol) and greater structural stability, robustness, and compactness than ribavirin (−4.87 kcal/mol). In cell-based assays, KHE suppressed HEV replication more effectively than ribavirin (IC₅₀: 12.75 μg/mL vs. 17.96 μg/mL), with no cytotoxicity at ≤100 μg/mL. Our results demonstrate that KHE exhibits potent anti-HEV activity by inhibiting both viral protease function and replication. Its mixed-type inhibition mechanism and favorable safety profile underscore its potential as promising source of lead candidate for HEV therapeutics.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"192 ","pages":"Article 107335"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148263897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isoquinoline alkaloids from Stephania japonica var. discolor and their neuroprotective effects against OGD/R-induced oxidative injury","authors":"Jiayi Du,&nbsp;Jiao Xiao,&nbsp;Di Zhou,&nbsp;Gang Chen,&nbsp;Yingjie Wang,&nbsp;Ning Li","doi":"10.1016/j.fitote.2026.107338","DOIUrl":"10.1016/j.fitote.2026.107338","url":null,"abstract":"<div><div><em>Stephania japonica</em> var. <em>discolor</em> is a traditional medicinal plant reported to contain alkaloids. In this study, eight isoquinoline alkaloids were isolated from the whole plant, including four previously undescribed compounds (<strong>1</strong>–<strong>3</strong>, <strong>6</strong>). These compounds were evaluated for neuroprotective effects using an oxygen-glucose deprivation/reperfusion (OGD/R) model in PC-12 cells. Several of the isolated alkaloids significantly improved cell viability and reduced intracellular reactive oxygen species (ROS) levels under OGD/R conditions. These results suggest that the neuroprotective activity of these compounds may be closely associated with their antioxidant properties. This is the first study to report the neuroprotective activity of isoquinoline alkaloids from <em>S. japonica</em> var. <em>discolor</em> and to explore their preliminary antioxidant mechanisms. The findings expand the chemical profile of this plant and highlight promising natural product-derived leads for the development of therapeutic agents against ischemic stroke.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"192 ","pages":"Article 107338"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148263853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multidimensional strategy for identifying quality markers of Radix ginseng-Schisandra chinensis in the disruption of the inflammation-cancer transformation process of hepatocellular carcinoma based on metabolic regulation and chemical properties","authors":"Saiyu Li,&nbsp;Yijing Dong,&nbsp;Rui Gu,&nbsp;Lifang Guan,&nbsp;Jiaqi Song,&nbsp;Mingzhe Zhang,&nbsp;Qian Zhang,&nbsp;Qing Li,&nbsp;Yiwen Zhang","doi":"10.1016/j.fitote.2026.107317","DOIUrl":"10.1016/j.fitote.2026.107317","url":null,"abstract":"<div><div>Comprehensive quality control is essential for ensuring the efficacy and safety of traditional Chinese medicines (TCMs). However, current quality control methods of TCMs primarily focus on quantifiable indicators, neglect the variation of pharmacodynamic constituents across disease progression. This study aimed to develop a comprehensive pathogenesis-oriented quality control framework for TCMs, using <em>Radix ginseng</em>-<em>Schisandra chinensis</em> (R-S) herb pair intervention in hepatocellular carcinoma (HCC) progression as an example. A rat model of “hepatitis-cirrhosis-HCC-advanced HCC” was established to evaluate the effects of R-S. Untargeted metabolomics revealed that bile acid (BA) dysregulation was the key pathological mechanism in HCC progression. Target metabolomics, liver-incorporated constituents analysis, immunoblotting techniques, correlation analysis and weighted average algorithm were integrated to screen the pharmacodynamic substances of R-S. HPLC fingerprinting of R-S was established and analyzed using machine learning (ML) algorithms to identify characteristic constituents. Ultimately, the Q-markers of R-S were ascertained and verified by molecular docking and molecular dynamics simulation. R-S effectively restored BA homeostasis during the progression in HCC by modulating the aberrant expression of BA-regulatory proteins (BSEP, MRP2, OATP1B1, NTCP1, CYP27A1, and CYP7A1). 6, 8, and 8 pharmacodynamic components of R-S were identified for preventing the further progression of hepatitis, cirrhosis, and HCC, respectively. Furthermore, 7 characteristic constituents of R-S were determined. Ultimately, 5 specific, measurable, and effectively Q-markers were identified. This study provides a reliable framework for facilitating precise quality control and applications of TCMs.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"192 ","pages":"Article 107317"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148210648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MALDI-MSI spatial mapping of phenanthrenes in Bletilla striata leaves uncovers BsLAC1 as the first laccase catalysing phenanthrene dimerisation","authors":"Lizhi Li,&nbsp;Jiayi Ye,&nbsp;Ruolin Geng,&nbsp;Jinfang Ye,&nbsp;Sheng Yao,&nbsp;Weichang Huang,&nbsp;Ping Xu,&nbsp;Chunping Tang,&nbsp;Yang Ye","doi":"10.1016/j.fitote.2026.107321","DOIUrl":"10.1016/j.fitote.2026.107321","url":null,"abstract":"<div><div><em>Bletilla striata</em>, a traditional Chinese medicinal herb, is particularly rich in phenanthrenes and their dimers, which exhibit anti-inflammatory, antibacterial, and antitumor properties. While phenanthrenes are biosynthesized from bibenzyl precursors through free radical oxidative coupling, and that monomers may further dimerize through radical reactions, the enzymatic basis of the dimerisation remains unexplored. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was employed to visualize the distribution of metabolites across four tissues—pseudobulbs, stems, petioles, and leaf tips. Bibenzyls, phenanthrenes, and their dimers exhibited distinct spatial patterns, with significantly stronger signals in leaf tips than in petioles. Quantitative analysis revealed that phenanthrene dimers were significantly more abundant in leaf tips, suggesting that enzymes promoting monomer coupling into dimers may be more active there. Given the well-established role of laccases in radical coupling reactions in plants, we hypothesized that these enzymes may catalyse phenanthrene dimerisation. In vitro assays confirmed that commercial laccases from <em>Trametes versicolor</em> and <em>Rhus verniciflua</em> catalysed phenanthrene dimer formation. Transcriptomic comparison between leaf tips and petioles, coupled with AlphaFold3-based structural modelling and homology alignment identified five candidate laccase genes, with Unigene10810 showing the highest similarity to the <em>R. verniciflua</em> laccase. Transient expression of Unigene10810 in <em>Nicotiana benthamiana</em> confirmed its catalytic activity, and it was designated as <em>Bletilla striata</em> laccase1 (BsLAC1). This study provides the first evidence of laccase-mediated radical oxidative coupling in phenanthrene dimer biosynthesis, enhancing our understanding of their biosynthetic pathway in <em>B. striata</em>.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"192 ","pages":"Article 107321"},"PeriodicalIF":2.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148155908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Decoding the heart-saving power of Sanweidoukou decoction: Mitochondrial and molecular insights” [Fitoterapia 190 (2026) 107134]","authors":"Hongyang Wang ,&nbsp;Yuting Sun ,&nbsp;Xi Yang ,&nbsp;Wutang Shi ,&nbsp;Wenyi Zhu ,&nbsp;Jingjing Bai ,&nbsp;Danli Yang ,&nbsp;Yu Wang","doi":"10.1016/j.fitote.2026.107183","DOIUrl":"10.1016/j.fitote.2026.107183","url":null,"abstract":"","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"190 ","pages":"Article 107183"},"PeriodicalIF":2.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147510769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Insight into the effect and mechanisms of ginsenosides and perilla seed extracts on hepatocellular bioactivities: Hypolipidemic, hypoglycemic, and antioxidant properties’ [Fitoterapia 187 (2025) 106932]","authors":"Shang Jia ,&nbsp;Huasong Bai ,&nbsp;Tong Liu ,&nbsp;Hengyan Wang ,&nbsp;Ye Yue ,&nbsp;Zhanzhong Wang","doi":"10.1016/j.fitote.2026.107151","DOIUrl":"10.1016/j.fitote.2026.107151","url":null,"abstract":"","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"190 ","pages":"Article 107151"},"PeriodicalIF":2.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147364582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical constituents and toxicological, anti-inflammatory, and antinociceptive assessment of Baliospermum effusum Pax et Hoffm.","authors":"Qing-Ying Hong,&nbsp;Zu-Hui Wang,&nbsp;Yi-Hang Ba,&nbsp;Yi-Huan Li,&nbsp;De-Xin Li,&nbsp;Hong-Ping He,&nbsp;Rui Yang,&nbsp;Hua-Yi Jiang","doi":"10.1016/j.fitote.2025.107078","DOIUrl":"10.1016/j.fitote.2025.107078","url":null,"abstract":"<div><div><em>Baliospermum effusum</em> Pax et Hoffm., a shrub known as “Baodongdian” in Dai folk medicine, has been traditionally employed for treating traumatic injuries, rheumatism, and jaundice. The present study investigated the acute toxicity, anti-inflammatory and antinociceptive properties, and phytochemical constituents of the 95 % ethanolic extract of <em>B</em>. <em>effusum</em> (EEBe). The toxicity assessment revealed that EEBe (2000 mg/kg) induced no adverse effects in behavior, mortality, body/organ weights, histopathology, or biochemical parameters over 14 d. Pharmacological assays on EEBe (150, 300, and 600 mg/kg) revealed that it effectively inhibited carrageenan-induced paw edema (<em>n</em> = 10), xylene-stimulated ear edema (<em>n</em> = 8), and acetic acid-triggered writhes (<em>n</em> = 8), while showing no significant antinociceptive activity in the hot-plate test (<em>n</em> = 8). ELISA (<em>n</em> = 8) showed that EEBe modulated serum IL-1<em>β</em>, IL-6, and IL-10 levels, while qRT-PCR (<em>n</em> = 5) demonstrated the downregulation of IL-1<em>β</em> and IL-6 mRNA expression. Furthermore, eight compounds including 4-hydroxy-2,3-dimethyl-2-nonen-4-olide (<strong>1</strong>), 22-dehydroclerosterol (<strong>2</strong>), <em>β</em>-sitosterol (<strong>3</strong>), vanillin (<strong>4</strong>), 1-linolenoylglycerol (<strong>5</strong>), 1-<em>O</em>-stearoyl-glycerol (<strong>6</strong>), bis(2,3-dihydroxypropyl)nonanedioate (<strong>7</strong>), and (3<em>S</em>,5<em>S</em>)-diverniciaoid A (<strong>8</strong>) were isolated from EEBe, with compounds <strong>5</strong> and <strong>7</strong> suppressing NO production in LPS-induced RAW 264.7 macrophages. These data revealed that EEBe was non-toxic at 2000 mg/kg and exhibited anti-inflammatory and antinociceptive properties, and that <strong>5</strong> and <strong>7</strong>, reported for the first time from this species, showed anti-inflammatory activity in vitro, collectively supporting the folk use of <em>B</em>. <em>effusum</em> for inflammatory and pain-related disorders. To our knowledge, this is the first report on the in vivo pharmacological activities and toxicity of <em>B</em>. <em>effusum</em>.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107078"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145892275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}