Fitoterapia最新文献

{"title":"Anti-inflammatory polyketides produced by Antarctic-derived fungus Aspergillus ochraceopetaliformis SCSIO 05702","authors":"Meng-Jing Cong ,&nbsp;Xue Ren ,&nbsp;Zi-Yi Wu ,&nbsp;Xiao-Yan Pang ,&nbsp;Yong-Hong Liu ,&nbsp;Peng Guo ,&nbsp;Jun-Feng Wang","doi":"10.1016/j.fitote.2026.107095","DOIUrl":"10.1016/j.fitote.2026.107095","url":null,"abstract":"<div><div>Five undescribed compounds (<strong>1</strong>–<strong>5</strong>), together with four known compounds (<strong>6</strong>–<strong>9</strong>) were isolated from the Antarctic-derived fungus <em>Aspergillus ochraceopetaliformis</em> SCSIO 05702. Their structures were elucidated by the nuclear magnetic resonance spectrum (NMR), mass spectrometry (MS). In addition, the absolute configurations of these compounds were determined by the combination of ECD and DP4⁺ calculations, chiral-phase HPLC analysis, Mosher's ester analysis and Mo₂(OAc)₄ induced circular dichroism method. Among them, <strong>5</strong> and <strong>6</strong> exhibited potent anti-inflammatory responses induced by LPS in RAW264.7 cells. Specifically, <strong>5</strong> and <strong>6</strong> were able to markedly inhibit the production of NO and pro-inflammatory cytokines including IL-6, TNF-<em>α</em>, and MCP-1 in RAW264.7 cells exposed to 0.1 μg/mL LPS. Moreover, they effectively upregulated the anti-inflammatory cytokines, such as IL-4, IL-10, and Arg-1 gene expression levels impaired by LPS without obvious cytotoxicity.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107095"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145988919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnolia officinalis Lignans induce apoptosis and epigenetic reprogramming via the miR-148a-3p/DNMT-1/UTF-1 axis in pancreatic cancer cells","authors":"Jinwon Choi ,&nbsp;Han-Saem Lee ,&nbsp;Hyo Jeong Kim ,&nbsp;Min Choi ,&nbsp;Trina E. Tallei ,&nbsp;Chi-Hoon Ahn ,&nbsp;Jai-Hyun So ,&nbsp;Moon Nyeo Park ,&nbsp;Bonglee Kim","doi":"10.1016/j.fitote.2026.107115","DOIUrl":"10.1016/j.fitote.2026.107115","url":null,"abstract":"<div><div><em>Magnolia officinalis</em> Rehder et Wilson (MRW) is a traditional herbal medicine with well-documented anti-inflammatory and antioxidative properties, yet its molecular basis in cancer therapy remains incompletely defined. This study aimed to elucidate the multi-target anticancer potential of MRW against pancreatic cancer through integrated in vitro and in silico analyses. LC–MS/MS profiling identified honokiol and magnolol as the major bioactive constituents, confirmed by retention time and UV spectra. MRW treatment suppressed cell viability and induced apoptosis in PANC-1 and MIA PaCa-2 cells by promoting reactive oxygen species (ROS) generation, mitochondrial membrane potential (∆Ψm) depolarization, and caspase activation, while sparing normal epithelial cells. Mechanistically, MRW inhibited DNA methyltransferase 1 (DNMT-1) and JAK2/STAT3 signaling while restoring undifferentiated embryonic cell transcription factor 1 (UTF-1) and miR-148a-3p expression, thereby reversing the epigenetic silencing and ROS overproduction characteristic of pancreatic cancer cells. Molecular docking further demonstrated strong binding affinities of honokiol, magnolol, and magnolin toward DNMT-1, UTF-1, STAT3, JAK2, IL-6, and Survivin, forming stable hydrogen-bond and π–π stacking interactions within catalytic pockets. These interactions suggest that MRW constituents' function as non-nucleoside DNMT-1 inhibitors and ROS–immune modulators that disrupt oncogenic feedback loops and re-activate apoptotic pathways. Collectively, these findings identify MRW as a multi-target phytomedicine integrating ROS-mediated oxidative stress, epigenetic remodeling, and immune–apoptotic signaling, supporting its translational potential as a low-toxicity adjunct strategy to conventional pancreatic cancer therapies.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107115"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146104519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of the hepatoprotective effects of the three processed products of Tetrastigma hemsleyanum Diels et Gilg: in vitro pharmacological investigation and integrating Network pharmacology","authors":"Zijin Xu ,&nbsp;Shaoxian Wang ,&nbsp;Hao Chen ,&nbsp;Tao Lv ,&nbsp;Zhiwen Zhang ,&nbsp;Faxiang Pu ,&nbsp;Zhangfu Xie ,&nbsp;Longfei Feng ,&nbsp;Ping Wang","doi":"10.1016/j.fitote.2025.107050","DOIUrl":"10.1016/j.fitote.2025.107050","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Tetrastigma hemsleyanum</em> Diels et Gilg (TDG), a traditional Chinese medicinal (TCM) plant, is historically used for liver injury treatment. Despite its ethnopharmacological significance, systematic studies on how processing affects its composition and efficacy remain lacking.</div></div><div><h3>Aim of the study</h3><div>This study aimed to compare the chemical profiles and hepatoprotective effects of extracts from TDG processed with three different Paozhi (TCM processing) methods (fresh, freeze-dried, and hot-air drying) and explore their mechanisms against drug-induced liver injury (DILI).</div></div><div><h3>Materials and methods</h3><div>Chemical constituents were analyzed <em>via</em> UPLC-Q-TOF-MS/MS. Hepatoprotection was evaluated using an acetaminophen (APAP)-induced human normal hepatocytes (LO2) cell model. Network pharmacology and molecular docking identified targets and pathways.</div></div><div><h3>Results</h3><div>Thirty-two compounds were identified across TDG extracts, with 13 shared and 19 unique to specific formulations. All extracts alleviated liver injury, but freeze-dried TDG (TDG-b) showed the strongest effect. Quercetin, procyanidin B1, catechins, kaempferol, and isorhamnetin emerged as key active constituents in TDG-b, targeting DILI through cancer, lipid and atherosclerosis, Hypoxia-Inducible Factor 1 (HIF-1), and Tumor Necrosis Factor (TNF) signaling pathways. Molecular docking confirmed robust binding between these compounds and core therapeutic targets.</div></div><div><h3>Conclusions</h3><div>TDG-b, a freeze-dried extract, optimally preserves bioactive constituents and demonstrates superior anti-DILI activity, validating traditional processing wisdom. This study bridges ethnopharmacological knowledge and mechanistic evidence, guiding TDG-based therapeutic optimization.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107050"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145827046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astragalus membranaceus improves blood glucose and renal function in diabetic kidney disease mice via gut microbial metabolite axis","authors":"Pei-Pei Zhang ,&nbsp;Ming-Yang Cui ,&nbsp;Shui-Yuan Yang ,&nbsp;Bo Han ,&nbsp;Wei Yu ,&nbsp;Tian-Tian Wei ,&nbsp;Ke-Wu Zeng ,&nbsp;Peng-Fei Tu","doi":"10.1016/j.fitote.2025.107048","DOIUrl":"10.1016/j.fitote.2025.107048","url":null,"abstract":"<div><div>Recent studies have demonstrated the therapeutic potential of <em>Astragalus membranaceus</em> in diabetic kidney disease (DKD); however, the underlying mechanisms remain incompletely elucidated. In this study, we established a streptozotocin-induced DKD mouse model to evaluate the effects of <em>A. membranaceus</em> extract (AME) on glycemic control, renal function, gut microbiota composition, and metabolic profiles. Biochemical analyzes revealed that <em>A. membranaceus</em> significantly attenuated hyperglycemia and improved renal function, as indicated by reduced serum creatinine and blood urea nitrogen levels. Metagenomic sequencing demonstrated that <em>A. membranaceus</em> reversed microbial dysbiosis by suppressing pathogenic bacteria (e.g., <em>Aerococcus urinaeequi</em>) and enriching beneficial probiotics (e.g., <em>Thomasclavelia cocleata</em>). Furthermore, LC/MS-based metabolomics identified key metabolic pathways, including glycerophospholipid metabolism and bile acid synthesis, as potential mediators of the therapeutic effects. These findings underscore the crucial role of the gut-renal axis in DKD pathogenesis and provide a mechanistic basis for the clinical application of <em>A. membranaceus</em>.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107048"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145818606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purpurin as a promising anticancer agent: A review of preclinical evidence","authors":"Fui-Ling Voon ,&nbsp;Yu Zhao Lee ,&nbsp;Xiao Ying Ooi ,&nbsp;Charlotte Zi Ern Tay ,&nbsp;Ji Wei Tan ,&nbsp;Chau Ling Tham ,&nbsp;Yu-Cheng Ho ,&nbsp;Ming Tatt Lee","doi":"10.1016/j.fitote.2025.107052","DOIUrl":"10.1016/j.fitote.2025.107052","url":null,"abstract":"<div><div>Purpurin, a naturally occurring anthraquinone pigment, has gained attention for its promising anticancer properties. This systematic-narrative hybrid review summarises current preclinical evidence on its mechanisms of action, pharmacology, and translational potential. Literature searches were conducted using PubMed, Web of Science, Scopus, and Google Scholar up to June 2025. Purpurin demonstrates selective cytotoxicity across multiple cancer models through redox imbalance, mitochondrial dysfunction, inhibition of PI3K/AKT signalling, and upregulation of the tumour suppressor LHPP. It also interferes with amino acid and glutamine metabolism and suppresses oncogenic protein aggregation. As a photosensitiser, purpurin enhances photodynamic therapy through light-activated ROS generation. Despite these promising mechanistic insights, its clinical applicability remains limited by poor aqueous solubility, rapid metabolism, and insufficient pharmacokinetic and toxicological data. Early <em>in vivo</em> studies indicate favourable safety, and emerging nanoparticle-based delivery systems show potential to improve bioavailability and tumour targeting. Collectively, current findings highlight purpurin as a compelling candidate for further development in oncology, particularly as part of combination or photo-enhanced therapeutic approaches. Continued research is required to address existing pharmacological gaps and to evaluate purpurin in clinically relevant models.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107052"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145801955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota combined with metabolomics approach to investigate the processing-based detoxification mechanism of processed cream of Croton tiglium L. seeds","authors":"Wen Pan ,&nbsp;Ze-Fei Jia ,&nbsp;Xiao-Bin Deng ,&nbsp;Liang-Ying Li ,&nbsp;Shu-Li Man ,&nbsp;Shi-Qiang Tan ,&nbsp;Jing Hu","doi":"10.1016/j.fitote.2025.107067","DOIUrl":"10.1016/j.fitote.2025.107067","url":null,"abstract":"<div><div><em>Croton tiglium</em> L. (Crotonis Fructus, CF), a classic toxic but valued Chinese herb used for over 2000 years, requires detoxification processing for clinical safety. Crotonis Semen Pulveratum (CP) is the most common processed product of CF, which has been utilized since the Song Dynasty. Nevertheless, the detoxification mechanisms underlying CP processing is still unclear. Therefore, this study investigated toxicity differences between raw and processed CF in rats and preliminarily predicted the potential attenuation mechanism after processing. UPLC-Q-Exactive-MS characterized CF and CP constituents, and 16S rRNA sequencing analyzed gut microbiota changes. Additionally, serum metabolomics was adopted to identify differential biomarkers and principal metabolic pathways associated with CP toxicity attenuation. The results show that chemical profiling identified 18 compounds that differentiated CP from CF. Histopathology showed processing significantly alleviated CF-induced gut damage in normal rats. CP restored colonic mucosa, reversing CF-induced damage to epithelium, crypts, and goblet cells. Moreover, CP treatment exhibited substantial regulatory effects on inflammatory and oxidative markers. Gut microbiota studies revealed that processing-mediated toxicity attenuation was closely associated with the restoration of gut microbial diversity and specific modulation of key bacterial taxa, including Eubacterium_coprostanoligenes_group, Escherichia-Shigella and Lactobacillus. Processing ameliorated CF-induced serum metabolic disorders, identifying 12 biomarkers linked to glycerolipid, lysine, and arginine/proline metabolism pathways. Additionally, quantitative analysis indicated that the reduction of crotonane diterpenoids after processing might contribute to the observed detoxification effects of CP. This study provides new insights into the possible processing-mediated toxicity attenuation of CP, offering a scientific foundation for its quality optimization and safe clinical application.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107067"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145846173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkamides from Piper nigrum and their potential inhibitory effect on NLRP3 inflammatory activation","authors":"Yadong Lv ,&nbsp;Guiping Wu ,&nbsp;Fenglin Gu ,&nbsp;Xin Lu ,&nbsp;Jinglin Zhang ,&nbsp;Zhiqiang Niu ,&nbsp;Xu Wang ,&nbsp;Fei Xu ,&nbsp;Fenglun Zhang ,&nbsp;Xiaode Huang ,&nbsp;Yunhe Lian ,&nbsp;Zhanjiao Wei ,&nbsp;Fu Li ,&nbsp;Haifeng Wu ,&nbsp;Weicheng Hu","doi":"10.1016/j.fitote.2026.107084","DOIUrl":"10.1016/j.fitote.2026.107084","url":null,"abstract":"<div><div>Four previously undescribed alkamides (<strong>1</strong>–<strong>4</strong>) were isolated from the fruits of <em>Piper nigrum</em>. Their chemical structures were elucidated using HRESIMS, NMR, and optical rotation analyses. A classical NLRP3 inflammasome activation model was established by priming macrophages with LPS followed by nigericin stimulation. Compounds <strong>1</strong>–<strong>4</strong> exhibited markedly stronger inhibition than the reference compound piperine. Molecular docking further revealed their binding modes within the NACHT domain of NLRP3. All four derivatives displayed higher docking scores than piperine, with compound <strong>2</strong> showing the strongest predicted binding affinity. Molecular dynamics simulations have verified that the molecule had good binding free energy with NLRP3 and indicated the key amino acids involved in the binding. These findings enrich the structural diversity of piperine derivatives and expand the molecular scaffold available for further structure–activity relationship studies, thereby providing a solid molecular basis for the rational design and synthesis of new piperine-derived NLRP3 inhibitors.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107084"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethyl acetate fraction of Striga hermonthica (Delile) Benth. inhibits AGEs-mediated inflammatory markers in THP-1 monocytes","authors":"Abdoulaye Segda ,&nbsp;Priya Tufail ,&nbsp;Roland Nâg-Tiéro Méda ,&nbsp;Aneela Fayaz ,&nbsp;Benjamin Kouliga Koama ,&nbsp;Georges Anicet Ouédraogo ,&nbsp;Humera Jahan ,&nbsp;M. Iqbal Choudhary","doi":"10.1016/j.fitote.2026.107082","DOIUrl":"10.1016/j.fitote.2026.107082","url":null,"abstract":"<div><div>The present study investigated the antiglycation activities of extracts and fractions of <em>Phyllanthus amarus</em>, <em>Chrysanthellum americanum</em>, <em>Striga hermonthica</em> and, based on cytotoxicity results, evaluated the anti-inflammatory potential of the ethyl acetate fraction of <em>Striga hermonthica</em> (<em>EtOAc-Sh</em>) in AGE-challenged THP-1 monocytes. The <em>in vitro</em> methylglyoxal (MGO)-bovine serum albumin (BSA) assay revealed notable inhibition of AGE formation by <em>EtOAc-Sh</em> (IC₅₀ = 100.1 ± 0.001 μg/mL; quercetin 1.23 μM, gallic acid 0.131 μM, rutin 0.0021 μM), with rutin used as the standard (IC₅₀ = 402 ± 0.30 μM). Cell metabolic assay showed <em>EtOAc-Sh</em> was non-cytotoxic to HepG2 hepatocytes (∼ 94 % cell viability at 250 μg/mL), and THP-1 monocytes (≥ 90 % cell viability at 500 μg/mL). Moreover, <em>EtOAc-Sh</em> significantly (<em>p &lt;</em> 0.001) reduced the AGE-mediated ROS production (83 % at 100 μg/mL), as compared to apocynin (69 % at 100 μM). Furthermore, <em>EtOAc-Sh</em> suppressed the NF-κB (p⁶⁵) (RFU: 9.18 at 100 μg/mL) activation, as compared to PDTC (RFU: 6.97) at 100 μM. <em>EtOAc-Sh</em> also significantly (<em>p</em> &lt; 0.001) reduced the COX-2 levels (1.52-fold decrease at 100 μg/mL; PDTC, 1.68-fold decrease) in THP-1 monocytes, while significantly (<em>p</em> &lt; 0.001) reversing the AGE-induced suppression of COX-1 levels (1.89-fold increase at 100 μg/mL; PDTC, 1.88-fold increase) at 100 μM. HPLC-UV analysis identified quercetin, gallic acid, and rutin, as the active constituents of the <em>EtOAc-Sh</em> fraction. These findings suggest that <em>EtOAc-Sh</em> fraction as a potential antiglycation, and anti-inflammatory agent, which supporting the traditional use of <em>Striga hermonthica</em> in diabetes management in Burkina Faso.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107082"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting of endophytic fungi from medicinal plants as a source of antimicrobial agents: In vitro evaluation, metabolite profiling, and docking-based elucidation","authors":"Shaymaa Ahmed Gouda ,&nbsp;E.A. Gamal ,&nbsp;Hanaa Mohamed Gouda ,&nbsp;Ahmed A.M.A. Selim","doi":"10.1016/j.fitote.2026.107104","DOIUrl":"10.1016/j.fitote.2026.107104","url":null,"abstract":"<div><div>The emergence of microbial resistance to antimicrobial agents emphasizes the need to discover new ones. The potential use of endophytic fungi as a source of antimicrobial agents and their mode of action has not been fully explored. Thus, this research aimed to evaluate the antimicrobial activity of 16 endophytic fungal species isolated from some plants collected from Wadi Hagul, Egypt, along with assessing the mechanism of action of the most active one. <em>Alternaria</em> E15 was the most effective fungus against <em>S. aureus</em> with an MIC of 321.5 μgmL⁻¹, <em>E. coli</em>, <em>A. niger</em> with MICs of 1250 μgmL⁻¹, and <em>C. albicans</em> with an MIC of 2500 μgmL⁻¹. It was identified morphologically and molecularly as <em>Alternaria alternata</em> (accession no. PX106371). Sorbitol protection and ergosterol-binding assays indicated that its ethyl acetate extract does not target the fungal cell wall but acts through direct ergosterol binding in the fungal membrane. LC/MS analysis of <em>A. alternata</em> extract revealed seventeen major compounds belonging to different antimicrobial chemical classes. The docking studies on CYP51 and Penicillin-Binding Protein 3 showed that Okanin4-(6-acetylglucoside) and alternariol (docking scores −11.957 and − 7.009, respectively) may inhibit fungal ergosterol biosynthesis, while Okanin4-(6-acetylglucoside) and altenusin may inhibit bacterial cell wall synthesis (docking scores −8.537 and − 8.019, respectively). This study highlights, for the first time to our knowledge, the investigation of Egyptian endophytic fungi isolated from certain plants as a potential source for antimicrobial products, with an understanding of the responsible compounds and their mechanisms supporting their potential use in developing novel medicinal applications.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107104"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New dihydrohomoisoflavones from Polygonatum sibiricum with neuroprotective activity","authors":"Yuan-Yuan Zhu ,&nbsp;Xin Meng ,&nbsp;Qing-Shan Chen ,&nbsp;Li-Li Zhang ,&nbsp;Hai-Xue Kuang ,&nbsp;Yang Liu ,&nbsp;Juan Pan ,&nbsp;Yan Liu","doi":"10.1016/j.fitote.2026.107110","DOIUrl":"10.1016/j.fitote.2026.107110","url":null,"abstract":"<div><div>Six new dihydroisoflavones (<strong>1–6</strong>) along with eight congeners (<strong>7–9</strong>, <strong>11–15</strong>) as well as a known chalcone derivative (<strong>10</strong>) were isolated from <em>Polygonatum sibiricum</em>. To enhance the specificity and efficiency of the separation process, a combination of MSDIAL and MassQL techniques was used alongside column chromatography with silica gel, ODS, and preparative HPLC. The structural elucidation of the compounds was accomplished via thorough spectral characterisation, encompassing techniques such as 1D and 2D NMR, HR-ESI-MS, IR, UV and ECD. These findings were further validated by comparing them with data from existing literature. Furthermore, the neuroprotective activity of all purified compounds was assessed using a PC12 cell model subjected to H₂O₂ induction. The results of our study indicated that compounds <strong>2</strong>, <strong>3</strong>, <strong>5</strong>, <strong>11</strong>, and <strong>12</strong> significantly alleviated H₂O₂-induced damage in PC12 cells, increasing cell viability to approximately 74%, 76%, 79%, 76%, and 72%, respectively, at 50 μM without exhibiting cytotoxicity.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107110"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146043763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}