Fitoterapia最新文献

{"title":"Study on chemical composition, plasma-absorbed components, and anti-inflammatory and antibacterial activity of Hypericum patulum","authors":"Qiya Zhang ,&nbsp;Meiqin Wang ,&nbsp;Li Jiang ,&nbsp;Yang Wang ,&nbsp;Yuan Lu ,&nbsp;Xue Ma ,&nbsp;Yongjun Li","doi":"10.1016/j.fitote.2025.107044","DOIUrl":"10.1016/j.fitote.2025.107044","url":null,"abstract":"<div><div>This study investigated the chemical composition of <em>Hypericum patulum</em> and its plasma metabolites in rats using ultra-high-performance liquid chromatography coupled to a Q-Exactive Plus Orbitrap mass spectrometer (UPLC-Q Exactive Plus Orbitrap MS). A total of 162 compounds were identified in the plant extract of <em>H. patulum</em>, encompassing 50 flavonoids, 32 organic acids, 26 xanthones, 12 polycyclic polyprenylated acylphloroglucinols (PPAPs), 10 amino acids, 8 coumarins, 5 alkaloids, 3 sugars, 2 terpenoids, 3 aldehydes, and 11 others. Analysis of rat plasma revealed 48 compounds, consisting of 20 prototypes (predominantly xanthones, organic acids, and flavonoids) and 28 metabolites generated via glucuronidation, methylation, and sulfation. In LPS-induced RAW 264.7 macrophages, <em>H. patulum</em> extract significantly inhibited NO release and suppressed the expression of pro-inflammatory mediators, including IL-6, TNF-<em>α</em>, COX-2, iNOS, and IL-1<em>β</em>. In vivo, quercetin and shikimic acid attenuated xylene-induced ear edema and inflammatory infiltration in mice, and also lowered serum levels of the pro-inflammatory cytokines TNF-<em>α</em>, IL-6, and IL-1<em>β</em>. Antibacterial testing revealed that shikimic acid demonstrated activity against a panel of pathogens, with minimum inhibitory concentration (MIC) of 13.00, 6.50, and 20.08 mg/mL against <em>Staphylococcus aureus</em>, <em>Escherichia coli</em>, and <em>β-hemolytic Streptococcus</em>, respectively. Vanillic acid showed MICs of 6.50 mg/mL against <em>S. aureus</em> and 3.25 mg/mL against <em>E. coli</em>, whereas quercetin inhibited <em>S. aureus</em> at a concentration of 3.25 mg/mL. These findings characterize the pharmacologically active components of <em>H. patulum</em>, thereby validating its traditional use and laying a foundation for future investigations into its therapeutic mechanisms.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107044"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145849585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bee bread as a natural shield against genetic damage: Investigation of antigenotoxic and antimutagenic potential","authors":"Karolina Matejczuk ,&nbsp;Piotr M. Kuś ,&nbsp;Piotr Szweda","doi":"10.1016/j.fitote.2025.107077","DOIUrl":"10.1016/j.fitote.2025.107077","url":null,"abstract":"<div><div>Bee bread (BB), a fermented bee product rich in bioactive compounds, such as aminoacids and peptides, vitamins and polyphenols, has garnered attention for its potential health benefits, particularly high antioxidant potential. However, its role in protecting genetic material from damage (antigenotoxic and antimutagenic activity) remains largely unexplored. This study provides the first comprehensive evaluation of both antigenotoxic and antimutagenic activities of Polish bee bread extracts in human HEK293 cells, integrating functional assays with advanced chemical profiling.</div><div>Twenty Polish BB samples were analyzed for antioxidant activity using DPPH Radical Scavenging Assay and Cellular Antioxidant Activity assays, revealing significant radical scavenging and intracellular ROS reduction capacities. UHPLC-DAD-QqTOF-MS profiling identified 71 compounds, predominantly flavonoids and phenolamides, known for their cytoprotective roles.</div><div>Importantly, comet assay results demonstrated that BB extracts significantly reduced methyl methanesulfonate (MMS)-induced DNA strand breaks, while cytokinesis-block micronucleus assay confirmed up to 91 % reduction in mutagenicity without intrinsic genotoxicity. Unlike previous studies that focused primarily on antioxidant activity, our work establishes bee bread as a natural chemoprotective agent against DNA damage and chromosomal instability, highlighting its potential in disease prevention. These findings expand the current understanding of bee bread bioactivity and position it as a promising candidate for functional food and nutraceutical applications.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107077"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145855107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory perylenequinones from the endolichenic fungus Phialocephala fortinii triggered by a histone deacetylase inhibitor","authors":"Ke Xu ,&nbsp;Xiao-Bin Li ,&nbsp;Yu-Liang Xu ,&nbsp;Fei Xie ,&nbsp;Hong-Xiang Lou","doi":"10.1016/j.fitote.2025.107051","DOIUrl":"10.1016/j.fitote.2025.107051","url":null,"abstract":"<div><div>Inclusion of the histone deacetylase (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) into the culture medium of the endolichenic fungus <em>Phialocephala fortinii</em> resulted in the separation of three new perylenequinones phialocephalarins <em>E</em>-G (<strong>1</strong>–<strong>3</strong>) and enhanced the production of the known compounds phialocephalarins A-B (<strong>4</strong>–<strong>5)</strong>. A comprehensive spectral analysis combined with electron circular dichroism (ECD) data has elucidated their structures. Phialocephalarin E (<strong>1</strong>) displayed anti-inflammatory activity with the maximum inhibition rate of 57.3 % ± 7.3 % (50 μM). Further molecular docking experiment revealed the affinity degree of compound <strong>1</strong> to the prostaglandin E2 receptor 4 (EP4R) and the molecular dynamics (MD) simulation results showed compound <strong>1</strong>-EP4R complex had relatively good binding stability.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107051"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145827027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yuxingcao formula suppresses acute erythroleukemia through inhibition of AKT1 and FLI1","authors":"Zhongyou Yang ,&nbsp;Jiankun Hong ,&nbsp;Wuling Liu ,&nbsp;Kunlin Yu ,&nbsp;Anling Hu ,&nbsp;Yi Kuang ,&nbsp;Eldad Zacksenhaus ,&nbsp;Xiao Xiao ,&nbsp;Jingrui Song ,&nbsp;Lei Huang ,&nbsp;Chunlin Wang ,&nbsp;Yanmei Li ,&nbsp;Yaacov Ben-David","doi":"10.1016/j.fitote.2025.107079","DOIUrl":"10.1016/j.fitote.2025.107079","url":null,"abstract":"<div><div>Acute erythroid leukemia (AEL), a rare form of acute myeloid leukemia (AML), is defined as type M6 under the FAB classification, and characterized by inhibition of terminal differentiation and rapid expansion of erythroid progenitors. Despite its poor prognosis, AEL responds well to chemotherapy. Yet, the frequent emergence of resistant clones is a major concern, necessitating the development of alternative therapeutic strategies to treat this rare disease. Yuxingcao formula (YXCF), which consists of 5 herbs, is used in Traditional Chinese Medicine to treat various diseases including cancer, although the underlying mechanisms are not fully understood. Herein, we shown that in an animal model of erythroleukemia induced by Friend virus, YXCF treatment strongly inhibits leukemia progression accompanied by induction of erythroid differentiation, apoptosis and cell cycle arrest. UPLC-MS/MS and network pharmacology analyses of YXCF identified 89 compounds, 20 of which are known to have anti-cancer activity. Molecular docking identified AKT1 as a potential target of one of these compounds, baicalein. In docking and CETSA analyses, baicalein binds AKT leading to inhibition of its phosphorylation and its target mTOR. Baicalein significantly inhibited proliferation of leukemic cells in culture associated with induction of apoptosis and cell cycle arrest as well as suppression of erythroleukemogenesis in vivo. YXCF is also inhibits the FLI1 oncogene, known to play a critical role in erythroleukemia, likely through kaempferol, another YXCF compound. Understanding the role of other components of YXCF may eventually enable the development of a combination drug therapy with optimal anti-leukemia activity for the treatment of AEL.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107079"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vladimiria souliei alleviated ethanol induced gastric ulcer through inhibition of RIP1-RIP3-MLKL necrosome activation","authors":"Xiaojing Wei ,&nbsp;Binjing Zhao ,&nbsp;Xinyi Jiang ,&nbsp;Lunli Lan ,&nbsp;Rui Wang ,&nbsp;Yuanyuan Li","doi":"10.1016/j.fitote.2026.107102","DOIUrl":"10.1016/j.fitote.2026.107102","url":null,"abstract":"<div><div><em>Vladimiria souliei(Franch.)Ling</em> is used as medicine with dried roots, which is a perennial herb in Asteraceae family. It has been generally applied as traditional Chinese medicine with relieving abdominal swelling or pain, and treatment of digestive system disease. However, the pharmacological mechanism of alleviating gastric ulcer (GU) for this herb were still unknown. UPLC-Q-TOF/MS was performed to analyze the main components of <em>Vladimiria souliei</em> extract (VSE). Based on the results of histopathological observation, cytokines assay, and immunohistochemistry staining, the therapeutic effect of VSE on ethanol-induced GU was appraised. Meanwhile, the CCK-8, LDH assay and crystal violet staining were assessed to evaluate the appropriate concentration of VSE <em>in vitro.</em> In GES-1 cells induced by ethanol, the cell viability, ROS and cell membrane damage assay were detected after the interference of VSE. Then, several pivotal proteins relating to necroptosis in GES-1 were evaluated through immunofluorescence staining to illustrate the further pharma-cological mechanisms. As a result, VSE significantly restrained the expansion of ulcer area, reduced the pepsin activity, restored the levels of SOD, decreased the levels of MDA, ROS, TNF-α, IL-1β, IL-6, and IL-18, ultimately ameliorated the gastric mucosa injury. Further analysis exhibited that VSE significantly inhibited the cellular necroptosis by down-regulating the levels of RIP1, RIP3 and MLKL. Conclusions: This research clarified that the VSE could improve gastric mucosal injury through inhibition of RIP1-RIP3-MLKL necrosome activation, which might provide scientific evidences on the application of <em>Vladimiria souliei</em> as a ethnic herb to ameliorate GU.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107102"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular crosstalk between herbal phytochemicals and microRNAs in electronic cigarette vapor: Implications for respiratory health","authors":"Putri Cahaya Situmorang ,&nbsp;Helen Helen ,&nbsp;Syafruddin Ilyas ,&nbsp;Pandu Surya Pangestu ,&nbsp;Syahputra Wibowo ,&nbsp;Kaniwa Berliani ,&nbsp;Alexander Patera Nugraha ,&nbsp;Khatarina Meldawati Pasaribu","doi":"10.1016/j.fitote.2026.107120","DOIUrl":"10.1016/j.fitote.2026.107120","url":null,"abstract":"<div><div>The increasing use of herbal electronic cigarettes raises concerns regarding their biological impact beyond conventional nicotine delivery. Although marketed as “natural” alternatives, their phytochemical vapors interact with molecular pathways that may influence respiratory health. This review summarizes current evidence on the molecular crosstalk between herbal phytochemicals and microRNAs (miRNAs) in the context of electronic cigarette vapor exposure, emphasizing their roles in oxidative stress, inflammation, and tissue remodelling. Literature was systematically examined to evaluate in vitro, in vivo, and bioinformatics findings on phytochemicals commonly incorporated in herbal e-liquids. Key molecular pathways (NF-κB, Nrf2/HO-1, MAPK, PI3K/Akt/mTOR, and TGF-β/Smad) and miRNA regulators were analyzed to elucidate mechanisms of lung injury and protection. The search strategy involved PubMed, Web of Science, and Scopus databases, using the keywords “herbal e-liquid,” “phytochemicals,” “lung injury,” “miRNA,” and “molecular pathway.” Evidence indicates that bioactive phytochemicals such as quercetin, linalool, and EGCG can modulate the expression of miRNAs (e.g., miR-21, miR-146a, miR-155, miR-210), thereby regulating inflammatory and oxidative responses. Crosstalk between these phytochemicals and miRNAs contributes to dual effects: mitigating reactive oxygen species and cytokine overproduction, while in some cases promoting pro-inflammatory or fibrotic signalling under chronic exposure. This bidirectional modulation highlights the complex balance between potential therapeutic benefits and risks of herbal vapor inhalation. Herbal electronic cigarettes cannot be considered risk-free. Their phytochemicals engage in intricate interactions with miRNA networks and molecular pathways that may influence respiratory health. Understanding this molecular crosstalk is essential for evaluating safety, guiding regulatory strategies, and identifying therapeutic prospects of phytochemical-based inhalation systems.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107120"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Six undescribed diterpenoids from the aerial parts of Siegesbeckia pubescens Makino and their anti-inflammatory activity","authors":"LingXia Qu,&nbsp;JinBo Li,&nbsp;Jiang Zhou,&nbsp;MeiHui Zhou,&nbsp;HaiXue Kuang,&nbsp;Liu Yang,&nbsp;Hai Jiang","doi":"10.1016/j.fitote.2025.107068","DOIUrl":"10.1016/j.fitote.2025.107068","url":null,"abstract":"<div><div>Six novel diterpenoids (<strong>1</strong>–<strong>6</strong>) and seventeen known compounds (<strong>7</strong>–<strong>23</strong>) were isolated from the aerial parts of <em>Siegesbeckia pubescens</em> Makino. The chemical structures of the new compounds were established through comprehensive spectroscopic characterization, including HR-ESI-MS and 1D/2D NMR analyses, and their absolute configurations were determined by ECD calculations. The anti-inflammatory effect of all compounds against lipopolysaccharide (LPS)-induced RAW 264.7 cells was tested. The results indicated that compounds <strong>2</strong>–<strong>18</strong> and <strong>20</strong>–<strong>23</strong> significantly suppressed nitric oxide (NO) production.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107068"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145849559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening 5-lipoxygenase inhibitors from Choerospondias axillaris via a novel enzyme immobilization targeted fishing technology","authors":"Shiyu Li ,&nbsp;Kai Xu ,&nbsp;Shanpeng Ma ,&nbsp;Xueying Qin ,&nbsp;Li Jiang ,&nbsp;Xiaohong Hou ,&nbsp;Zibo Dong ,&nbsp;Xun Gao ,&nbsp;Lingling Huo","doi":"10.1016/j.fitote.2025.107047","DOIUrl":"10.1016/j.fitote.2025.107047","url":null,"abstract":"<div><div>Currently, coronary heart disease (CHD) is prevalent worldwide, with its harmful impact second only to that of malignant tumors. A growing number of studies have confirmed that <em>Choerospondias axillaris</em> can significantly improve the therapeutic effect of CHD treatment and reduce the incidence of adverse reactions. However, its bioactive components and pharmacological mechanisms remain unclear. Based on a targeted fishing strategy, this study synthesized magnetic nanomaterials immobilized with 5-lipoxygenase (5-LOX) for screening and identifying 5-LOX inhibitors from the crude extract of <em>Choerospondias axillaris</em>. A total of 21 active components were identified and analyzed using ultra-high performance liquid chromatography-Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS/MS). By comparing the screened active components with reported 5-LOX inhibitors, 9 novel 5-LOX inhibitors were discovered. Further molecular docking was performed on these 9 inhibitors, and the pharmacological activity of the selected ligands was verified using a 5-LOX inhibitor screening kit (fluorescence method).The compounds are ranked by activity from strongest to weakest: Lobetyolin (23.92 μM), Anhydroicaritin (29.55 μM), Narirutin(33.66 μM), Fisetin (47.28 μM) and Naringin(59.02 μM). This novel enzyme-immobilized targeted fishing technology is expected to efficiently and accurately screen bioactive components targeting different receptors from complex substrates.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107047"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145849551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of anti-tumor research on natural products combined with doxorubicin","authors":"Yanqing Wu,&nbsp;Jianguo Gao,&nbsp;Yuwei Zhao,&nbsp;Yingzhi Lu,&nbsp;Xiaoqing Cai","doi":"10.1016/j.fitote.2025.107076","DOIUrl":"10.1016/j.fitote.2025.107076","url":null,"abstract":"<div><div>Cancer represents a major global public health challenge, with its incidence and mortality rates increasing annually. Doxorubicin (DOX) is a commonly used chemotherapeutic agent. Despite its marked antitumor efficacy, the clinical application of DOX is limited by drug resistance and cumulative toxicity. Numerous natural products have garnered significant attention owing to their favorable antitumor, antioxidant, anti-inflammatory, and anti-apoptotic properties, and are increasingly being developed as adjuvant agents in combination therapy with DOX. The co-administration of natural products with DOX has emerged as a promising strategy to enhance therapeutic outcomes while mitigating adverse effects. However, this combination approach is constrained by poor drug solubility and disparate pharmacokinetic profiles. The advent of multifunctional nanodelivery systems has improved drug bioavailability and tumor targeting, thereby laying the groundwork for synergistic interactions between natural products and DOX. This review systematically summarizes the antitumor mechanisms and toxic side effects of DOX, elucidates the mechanisms of action and structure-activity relationships of certain natural products serving as DOX sensitizers and protective agents, and highlights recent advances in nano-platform-based co-delivery strategies, offering valuable insights for future cancer therapeutics.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107076"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145855128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of different stinging nettle preparations regarding to their immunomodulating effect on primary human T-lymphocytes","authors":"Adrian Wilmers ,&nbsp;Olivier Potterat ,&nbsp;Roman Huber ,&nbsp;Stefanie Kowarschik","doi":"10.1016/j.fitote.2025.107081","DOIUrl":"10.1016/j.fitote.2025.107081","url":null,"abstract":"<div><div><em>Urtica dioica</em> L. (<em>U. dioica</em>) has shown anti-inflammatory effects and is regarded as a potential natural agent for treating chronic inflammatory conditions. The differential effects of <em>U. dioica</em> preparations have not yet been investigated. The influence of nine different <em>U. dioica</em> preparations (teas, extracts, juice and tincture) on proliferation, apoptosis/necrosis and viability of primary human T cells were analyzed with standard methods. The effect on T lymphocytes was evaluated via cluster of differentiation (CD)25/CD69 marker expression, degranulation assays and the production of pro-inflammatory cytokines. Using Jurkat reporter cell lines, an influence on transcription factors was analyzed. <em>U. dioica</em> preparations selectively modulate T cell activation by downregulating the key activation markers CD25 and CD69. In addition, they reduce the production of the pro-inflammatory cytokine interleukin-2, inhibit T cell degranulation and suppress nuclear factor of activated T-cells (NFAT)-dependent transcription. The results highlight the potential of <em>U. dioica</em> as a natural agent, capable to modulate T cell-mediated immune responses. The immunomodulating effect differs between the extracts and preparations due to extraction methods and used plant material.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"189 ","pages":"Article 107081"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}