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Isolation of anti-inflammatory and cytotoxic secondary metabolites from Valeriana phu and evaluation of their mechanisms of action. 缬草抗炎和细胞毒性次生代谢物的分离及其作用机制的评价。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-06 DOI: 10.1016/j.fitote.2025.106377
Cansel Çelik, Özge Doğa İdiş, Yağmur Özhan, Dilruba Tirpanlar, Naz Unal, Burcin Gungor, Başak Aru, Enise Ece Gurdal, Wolfgang Sippl, Hande Sipahi, Hasan Kırmızıbekmez
{"title":"Isolation of anti-inflammatory and cytotoxic secondary metabolites from Valeriana phu and evaluation of their mechanisms of action.","authors":"Cansel Çelik, Özge Doğa İdiş, Yağmur Özhan, Dilruba Tirpanlar, Naz Unal, Burcin Gungor, Başak Aru, Enise Ece Gurdal, Wolfgang Sippl, Hande Sipahi, Hasan Kırmızıbekmez","doi":"10.1016/j.fitote.2025.106377","DOIUrl":"10.1016/j.fitote.2025.106377","url":null,"abstract":"<p><p>As a result of anti-inflammatory activity-guided fractionation, 16 secondary metabolites from the underground parts of Valeriana phu L. were obtained, including five new ones belonging to iridoid (1, 2, and 5), phenylpropanoid (6) and neolignan (7) chemical classes. Their structures were elucidated by 1D and 2D NMR analyses as well as HRESIMS. The in vitro anti-inflammatory activities of the extract, fractions and isolates were evaluated through NO inhibition assay on LPS-induced RAW 264.7 cells. Compounds 1-3, 7-9, 11, 13, and 16 which significantly inhibited the nitrite release (IC<sub>50</sub> 14.94-94.81 μM) were also assessed for their reducing capacity on TNF-α, IL-1β, IL-6, PGE2 and COX-2 production. Compounds 3, 8, and 16 inhibited LPS induced iNOS expression levels in Western blotting. Molecular docking studies for the active compounds targeting iNOS, TNF-α and COX-2 were also carried out. Moreover, compounds with remarkable anti-inflammatory activities were tested for their potential cytotoxicity against breast (MCF-7 and MDA-MB-231), glioblastoma (U87 and A172), pancreas (MIA PaCa-2 and PANC-1), hepatocellular (Mahlavu and Hep3B) cancer cell lines by WST-8. Compounds, 7, 8, and 16 showed significant cytotoxicity against A172 and PANC-1 cell lines (IC<sub>50</sub> 18.3-21.8 μM) via causing cell cycle arrest, especially in the G2/M phase and triggering the apoptotic pathway.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106377"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid isolation of cytotoxic daphnane diterpenoids from Daphne altaica Pall. using MS-DIAL. 快速分离达芙妮中具有细胞毒性的水仙烷二萜。使用MS-DIAL。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-06 DOI: 10.1016/j.fitote.2025.106385
Zi-Han Xi, Ning-Ning Du, Yan-Jiao Qiao, Gu-Xue Zhang, Guo-Dong Yao, Xiao-Xiao Huang, Shao-Jiang Song
{"title":"Rapid isolation of cytotoxic daphnane diterpenoids from Daphne altaica Pall. using MS-DIAL.","authors":"Zi-Han Xi, Ning-Ning Du, Yan-Jiao Qiao, Gu-Xue Zhang, Guo-Dong Yao, Xiao-Xiao Huang, Shao-Jiang Song","doi":"10.1016/j.fitote.2025.106385","DOIUrl":"10.1016/j.fitote.2025.106385","url":null,"abstract":"<p><p>Daphnane diterpenoids occurring in plants of the Thymelaeaceae are the focus of natural product drug discovery because of the wide range of their therapeutically biological activities. Considering the limited occurrence in some plants of the Thymelaeaceae, it is imperative to design a strategy for the target isolation of daphnane diterpenoids. In this study, a strategy was developed to filter the data using MZmine, generate the molecular network using the Global Natural Product Social Molecular Network Platform (GNPS), and determine the retention time of target compounds using MS-DIAL. Under the guidance of the approach which integrates the analysis of LC-MS/MS, compounds 1-5, representative diterpenoids from Daphne altaica Pall., were isolated. Their structures were determined through detailed spectroscopic analyses and ECD calculations. The growth-inhibitory activities of the isolated compounds against MCF-7, A549, and HepG2 cell lines was examined. Notably, compound 1 demonstrated the most noticeable cytotoxicity, exhibiting potent growth inhibition activities against A549 and HepG2 cells with IC<sub>50</sub> values of 2.89 μM and 5.30 μM, respectively. Further morphological and staining analyses corroborated that compound 1 induced apoptosis in A549 and HepG2 cells, highlighting its potential as a bioactive agent.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106385"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing nature's arsenal: Targeting the TGF-β/Smad Cascade with novel natural anti-fibrotic agents. 利用自然的武器库:用新的天然抗纤维化药物靶向TGF-β/Smad级联。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-06 DOI: 10.1016/j.fitote.2024.106372
Maohua Chen, Linjiang Song, Anqi Zeng
{"title":"Harnessing nature's arsenal: Targeting the TGF-β/Smad Cascade with novel natural anti-fibrotic agents.","authors":"Maohua Chen, Linjiang Song, Anqi Zeng","doi":"10.1016/j.fitote.2024.106372","DOIUrl":"10.1016/j.fitote.2024.106372","url":null,"abstract":"<p><strong>Background: </strong>Hepatic fibrosis is a wound healing response that leads to excessive deposition of extracellular matrix (ECM) due to sustained liver injury. Hepatic stellate cells (HSCs) are key players in ECM synthesis, with the TGF-β/Smad signaling pathway being central to their activation. Despite advances in understanding the pathogenesis of hepatic fibrosis, effective anti-fibrotic therapies are still lacking.</p><p><strong>Methods: </strong>This treatise conducts a comprehensive review of the literature on the hepatoprotective effects of natural products, including natural medicine compounds, herbal extracts, and polysaccharides. The focus is on their ability to modulate the TGF-β pathway, which is critical in the activation of HSCs and ECM synthesis in hepatic fibrosis.</p><p><strong>Results: </strong>The review identifies a variety of natural products that have shown promise in inhibiting the TGF-β/Smad signaling cascade, thereby reducing the activation of HSCs and ECM accumulation. These findings highlight the potential of these natural products as therapeutic agents in the treatment of hepatic fibrosis.</p><p><strong>Conclusions: </strong>The exploration of natural products as modulators of the TGF-β pathway presents a novel avenue for both clinical and preclinical research into hepatic fibrosis. Further investigation is warranted to fully understand the mechanisms of action and to develop these compounds into effective anti-fibrotic pharmaceuticals.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106372"},"PeriodicalIF":2.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative metabolomic study of twelve Acacia species by UHPLC-q-tof-ESI-MS coupled with chemometrics in correlation with antibacterial activity. UHPLC-q-tof-ESI-MS联合化学计量学对12种金合欢植物抗菌活性的比较代谢组学研究。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-05 DOI: 10.1016/j.fitote.2025.106378
Salma Elshamy, Nikolai Kuhnert, Mohamed El-Shazly, James Ziemah, Heba Handoussa
{"title":"Comparative metabolomic study of twelve Acacia species by UHPLC-q-tof-ESI-MS coupled with chemometrics in correlation with antibacterial activity.","authors":"Salma Elshamy, Nikolai Kuhnert, Mohamed El-Shazly, James Ziemah, Heba Handoussa","doi":"10.1016/j.fitote.2025.106378","DOIUrl":"https://doi.org/10.1016/j.fitote.2025.106378","url":null,"abstract":"<p><p>Genus Acacia comprises around 1500 species. They are widely used to treat inflammation as well as bacterial and fungal infections as they are enriched in phytochemicals, especially phenolics. The aim of this study was to evaluate the antibacterial activity of leaves' methanolic extracts of twelve Acacia species growing in Egypt against Vibrio parahaemolyticus, Salmonella enterica, Listeria monocytogens, Klebsiella pnemoniae, Bacillus aquimaris, Bacillus subtilis, and Escherichia coli. These species are Acacia nilotica (wild and cultivated), Acacia seyal, Acacia auriculiformis, Acacia saligna, Acacia xanthophloea, Acacia tortilis subsp. raddiana (Gabal Elba and Aswan), Acacia tortilis, Acacia laeta (wild and cultivated), and Acacia albida. Furthermore, to study the metabolomic composition and variation among these species using ultra-high-performance liquid chromatography-electrospray ionization quadrupole time of flight mass spectrometry (UHPLC-q-tof-ESI-MS) coupled with multivariate statistical analysis and correlate it to the antibacterial potential. Results showed that Acacia nilotica (AN) has superior antibacterial activity over the other species. In addition, it exhibited a distinct segregation in Principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA). Full profiling of AN using UHPLC-ESI-q-tof-MS revealed 42 phenolics mainly catechins. It was further subjected to bio-guided fractionation and revealed the presence of methyl gallic acid, gallic acid, catechin gallate, and digallate isomers in its most bioactive fraction. These compounds were identical to the compounds annotated as VIPs and were responsible for the segregation of AN in both PCA and PLS-DA analyses. Hence, this study sheds light on the use of chemometrics as an early tool for the detection of bioactive compounds.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"181 ","pages":"106378"},"PeriodicalIF":2.5,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects on neuroprotection and α-synuclein expression of a Canna coccinea rhizome extract. 美人蕉根茎提取物对神经保护作用及α-突触核蛋白表达的影响。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-05 DOI: 10.1016/j.fitote.2025.106380
Viviana G Spotorno, Mariana Roxo, Bernhard Wetterauer, Osvaldo A Zabal, Michael Wink
{"title":"Effects on neuroprotection and α-synuclein expression of a Canna coccinea rhizome extract.","authors":"Viviana G Spotorno, Mariana Roxo, Bernhard Wetterauer, Osvaldo A Zabal, Michael Wink","doi":"10.1016/j.fitote.2025.106380","DOIUrl":"https://doi.org/10.1016/j.fitote.2025.106380","url":null,"abstract":"<p><p>Parkinson's disease (PD) is the second most common neurodegenerative disorder in the elderly, currently with no cure. Its mechanisms are not well understood, however α-synuclein protein aggregation plays a central role in the pathogenesis of PD, leading to neurodegeneration. We demonstrated that in a PD model dietary in Caenorhabditis elegans treatment with an extract from the rhizome of Canna coccinea decreased the accumulation of α-synuclein. The extract showed low toxicity to neuro 2a cells and protected them from oxidative damage with H<sub>2</sub>O<sub>2</sub> as a model for neurodegeneration. We studied the chemical profile of the extract using liquid chromatography couple to Mass Spectrometry. In order to characterize the herbal extract, we quantified rosmarinic acid as a marker compound and measured its antioxidant activity in vitro. Altogether, apart from its potential as a functional food, Canna coccinea may be an interesting source of secondary metabolites in the development of potential novel anti-neurodegenerative drugs.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"181 ","pages":"106380"},"PeriodicalIF":2.5,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diterpenoids from the bark of Torreya grandis and their cytotoxicity activity. 香榧树皮中的二萜类化合物及其细胞毒性活性。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-04 DOI: 10.1016/j.fitote.2025.106376
Yu-Fan Meng, Jing-Jing Yuan, Mao-Sheng Zhang, Da-Le Guo, Pan-Xiang Cao, Jian-Wen Yang, Min-Jian Dong, Jun Dai, Cheng-Xin Sun, Shi-Ji Xiao
{"title":"Diterpenoids from the bark of Torreya grandis and their cytotoxicity activity.","authors":"Yu-Fan Meng, Jing-Jing Yuan, Mao-Sheng Zhang, Da-Le Guo, Pan-Xiang Cao, Jian-Wen Yang, Min-Jian Dong, Jun Dai, Cheng-Xin Sun, Shi-Ji Xiao","doi":"10.1016/j.fitote.2025.106376","DOIUrl":"https://doi.org/10.1016/j.fitote.2025.106376","url":null,"abstract":"<p><p>Four previously undescribed diterpenoids (2-5) were isolated from the bark of Torreya grandis, along with ten known analogues. The structures of the compounds were elucidated using NMR, HR-ESIMS, and ECD calculation. The cytotoxic effects of the isolated compounds on HCT-116, AsPC-1, and HepG2 cells were evaluated using the MTT assay. Compound 6 exhibited significant cytotoxicity against HepG2 cells. Further experiments revealed that compound 6 could arrest the G2 phase process in HepG2 cells through the JAK2/STAT3 signaling pathway.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"181 ","pages":"106376"},"PeriodicalIF":2.5,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolomics and dereplication strategies for the study of bioactive metabolites derived from plants and fungi. 植物和真菌生物活性代谢物研究的代谢组学和去复制策略。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-03 DOI: 10.1016/j.fitote.2024.106375
Paula Carolina Pires Bueno, Karen de Jesus Nicácio, Daniela Aparecida Chagas-Paula
{"title":"Metabolomics and dereplication strategies for the study of bioactive metabolites derived from plants and fungi.","authors":"Paula Carolina Pires Bueno, Karen de Jesus Nicácio, Daniela Aparecida Chagas-Paula","doi":"10.1016/j.fitote.2024.106375","DOIUrl":"https://doi.org/10.1016/j.fitote.2024.106375","url":null,"abstract":"","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106375"},"PeriodicalIF":2.5,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of the chemical profiles and biological activities of Thesium bertramii Azn. Extracts using a combination of in vitro, in silico, and network pharmacology methods. bertramii Azn的化学特征和生物活性的表征。采用体外、硅片和网络药理学相结合的方法提取。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-01 Epub Date: 2024-12-05 DOI: 10.1016/j.fitote.2024.106329
Eulogio J Llorent-Martínez, Sakina Yagi, Gokhan Zengin, Mehmet Veysi Cetiz, Abdullahi Ibrahim Uba, Ozgur Yuksekdag, Bengusu Hacer Akgul, Evren Yildiztugay, Ismail Koyuncu
{"title":"Characterization of the chemical profiles and biological activities of Thesium bertramii Azn. Extracts using a combination of in vitro, in silico, and network pharmacology methods.","authors":"Eulogio J Llorent-Martínez, Sakina Yagi, Gokhan Zengin, Mehmet Veysi Cetiz, Abdullahi Ibrahim Uba, Ozgur Yuksekdag, Bengusu Hacer Akgul, Evren Yildiztugay, Ismail Koyuncu","doi":"10.1016/j.fitote.2024.106329","DOIUrl":"10.1016/j.fitote.2024.106329","url":null,"abstract":"<p><p>The genus Thesium, family Santalaceae, comprises about 350 species, and, although many of them are used as functional food and in traditional medicine, there are limited studies evaluating their pharmacological potential. The present study was designed to evaluate the chemical profile, antioxidant, and enzyme inhibition potential of aerial parts and roots of T. bertramii Azn. Extracts were rich in phenolics: MeOH and aqueous extracts of the aerial parts showed the highest total phenolic and flavonoid contents and the best antioxidant activity in most assays. Ethyl acetate extracts of both organs exerted comparable anti-butyrylcholinesterase activity, while their methanol extracts displayed comparable anti-tyrosinase activity. The highest acetylcholinesterase inhibitory activity was recorded from the root's ethyl acetate extract, while that of the aerial parts revealed the best α-amylase and α-glucosidase inhibitory activity. Chemically, the aerial parts were dominated by quercetin derivatives, feruloylquinic acids, caffeoylquinic acids, and elenolic acid glucoside. Roots showed a lower diversity of compounds with elenolic acid, quercetin glycoside, and kaempferol glycoside as major compounds. Additionally, network pharmacology analyses (KEGG and STRING) identified critical molecular pathways and hub genes, including IL6, TNF, BCL-2, and JUN, indicating the multi-target potential of T. bertramii in cancer and cardiovascular diseases. In conclusion, this study assessed the chemical and biological properties of T. bertramii for the first time, and the obtained results indicated the potential of this species as a valuable source of bioactive molecules for the pharmaceutical, food, and cosmetic industries.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106329"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142784956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A review of the traditional uses, phytochemistry, pharmacology and toxicity for the genus Geum (Rosaceae). 综述了蔷薇科金属植物的传统用途、植物化学、药理和毒性。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-01 Epub Date: 2024-12-09 DOI: 10.1016/j.fitote.2024.106333
Xiyan Mo, Yangkun Zhou, Meng Zhan, Ying Zhang, Jun Liu, Hongfeng Quang, Lin Dong
{"title":"A review of the traditional uses, phytochemistry, pharmacology and toxicity for the genus Geum (Rosaceae).","authors":"Xiyan Mo, Yangkun Zhou, Meng Zhan, Ying Zhang, Jun Liu, Hongfeng Quang, Lin Dong","doi":"10.1016/j.fitote.2024.106333","DOIUrl":"10.1016/j.fitote.2024.106333","url":null,"abstract":"<p><p>The genus Geum (family Rosaceae) comprises about 70 species, of which several species have been characterized from Eurasia as notably as folk medicines for the treatment of anti-inflammatory, antiseptic, diuretics and astringent. Phytochemical analysis indicated that Geum species produce monoterpenoids, sesquiterpenes, triterpenoids, flavonoids, tannins, phenylpropanoids, and others compounds. Additionally, modern studies have found they possess diverse pharmacological activities such as antioxidant, anti-inflammatory, neuroprotective, antimicrobial, anti-diabetic, cardiovascular, and antitumor effects. Regardless of the remarkable medical potential of Geum extracts, clinical studies are limited and need to be performed to produce safer and less expensive plant-based drugs. This literature review aims to provide a comprehensive overview of Geum species, covering their traditional uses, phytochemistry, and pharmacological activities, and to summarize the current research status to better understand the application value of Geum plants in modern phytotherapy.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106333"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing medicinal proteins production in plant bioreactors: A focal review on promoters. 提高植物生物反应器中药用蛋白质的产量:关于促进剂的重点综述。
IF 2.5 3区 医学
Fitoterapia Pub Date : 2025-01-01 Epub Date: 2024-12-10 DOI: 10.1016/j.fitote.2024.106338
Cai Xiaoqi, Ning Kang, Xu Pei
{"title":"Enhancing medicinal proteins production in plant bioreactors: A focal review on promoters.","authors":"Cai Xiaoqi, Ning Kang, Xu Pei","doi":"10.1016/j.fitote.2024.106338","DOIUrl":"10.1016/j.fitote.2024.106338","url":null,"abstract":"<p><p>The use of plant bioreactors for the production of medicinal proteins has emerged as a promising and cost-effective alternative to traditional microbial and mammalian cell culture systems. This review provides a focused examination of the critical role of promoters in enhancing the production of therapeutic proteins within plant-based platforms. We discuss the latest advancements in promoter discovery, modification, and optimization for the expression of medicinal proteins in plants. The review highlights the challenges and opportunities associated with various types of promoters, including constitutive, tissue-specific, and inducible promoters, and their impact on medicinal protein yield and quality. Case studies are presented to illustrate the successful application of these promoter engineering techniques in plant bioreactors, emphasizing the potential for scalable and sustainable production of pharmaceutical proteins. Additionally, we explore the strategies for improving promoter function. This review is intended to guide researchers and industry professionals in the selection and design of promoters for the enhanced production of medicinal proteins in plant bioreactors.</p>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":" ","pages":"106338"},"PeriodicalIF":2.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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