Fitoterapia最新文献

{"title":"Aloe Vera flowers extracts inhibit melanogenesis via activating PI3K/Akt signaling pathway: Network pharmacology and experimental validation","authors":"Jiaxin Li ,&nbsp;Wei Zou ,&nbsp;MingKun Li ,&nbsp;Yongtao Yan ,&nbsp;Yue Yu ,&nbsp;Xiaomin Li ,&nbsp;Yan-Long Ma","doi":"10.1016/j.fitote.2025.106530","DOIUrl":"10.1016/j.fitote.2025.106530","url":null,"abstract":"<div><div><em>Aloe vera</em> is a traditional herbal medicine that has been used for thousands of years. However, its flowers are commonly considered useless and discarded. Although, a few recent studies find <em>Aloe vera</em> flowers extracts (AVFE) with ability to inhibit tyrosinase (TYR) activity <em>in vitro</em>, systematic research on AVFE as skin whitening agent against melanogenesis <em>in vitro</em> and <em>in vivo</em> is yet lack. Therefore, the anti-TYR activity and anti-melanogenic effects of AVFEs <em>in vitro</em> B16F10 cell model and <em>in vivo</em> zebrafish embryo model, analysis of all-components of AVFE, network pharmacology and protein expression of melanogenesis-related signaling pathways were well conducted in this study. The results demonstrated that AVFE could significantly reduce cellular TYR activity and melanin content <em>via</em> activating the phosphorylation of p-Akt and p-GSK-3β in PI3K/Akt signaling pathway, followed by reducing the phosphorylation of MITF and then downregulation of TYR, TRP1 and TRP2 expression levels, and finally decreasing TYR, TRP1, TRP2 and melanin syntheses. In addition, 24 components of AVFE were most likely responsible for its skin depigmentation. In conclusion, this study provides a basis for understanding the mechanism of AVFE on skin depigmentation, as well as a potential for applying AVFE as skin whitening agent in cosmetics.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106530"},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The hepatoprotective effect of Lonicera japonica Flos on rats with high-fat diet-induced non-alcoholic fatty liver disease","authors":"Xue Ma,&nbsp;Chun-Yong Sun,&nbsp;Yan Zhang,&nbsp;Jia Li,&nbsp;Dong-Sheng Zhao","doi":"10.1016/j.fitote.2025.106516","DOIUrl":"10.1016/j.fitote.2025.106516","url":null,"abstract":"<div><div><em>Lonicerae Japonica</em> Flos (LJF) is an edible-medicinal herb, rich in phenolic acids, flavonoids, iridoids and other bioactive ingredients, that has anti-inflammatory, antioxidant, antilipemic, and hepatoprotective effects. However, its effect on non-alcoholic fatty liver disease (NAFLD) remains to be elucidated. The aim of this study was to determine the effect of LJF on liver injury in rats with high-fat diet (HFD)-induced NAFLD. The administration of LJF extract to rats with HFD-induced NAFLD significantly improved their body weight and daily food intake, liver tissue steatosis, lipid droplet vacuolization, and inflammatory cell infiltration. In addition, the LJF extract also improved to varying degrees the serum biochemical parameters, liver lipid content, levels of inflammatory factors, and oxidative stress markers. Among the treatment groups, the LJF high-dose group (LJF-H) showed the most significant improvement effect. Additionally, the correlation matrix heatmap visualization indicated that LJF may ameliorate NAFLD mainly by lowering liver lipid content and improving serum biochemical parameters.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106516"},"PeriodicalIF":2.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absorption, distribution, metabolism, and excretion (ADME) of R,S-Goitrin in Radix Isatidis in rats by LC-MS/MS determination","authors":"Jia Tang ,&nbsp;Lisha Ye ,&nbsp;Baolian Wang","doi":"10.1016/j.fitote.2025.106514","DOIUrl":"10.1016/j.fitote.2025.106514","url":null,"abstract":"<div><div><em>Radix Isatidis</em> (syn. Isatis indigotica Fort.) is employed in the treatment of fever, influenza, acute tonsillitis, viral hepatitis, and COVID-19, demonstrating diverse pharmacological activities, including antibacterial, antiviral, antioxidant, anticancer, and immune-regulatory effects. Furthermore, there is significant potential for the development of new clinical applications. In order to investigate the pharmacokinetic characteristics, a quantitative method for determining R,S-goitrin in rat plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. The established LC-MS/MS method was employed to investigate the pharmacokinetics, tissue distribution, plasma protein binding, excretion, and metabolic characteristics of <em>Radix Isatidis</em> extract following oral administration in rats. Meanwhile, by comparing with the oral monomer group, the absorption profile of the extract in rats was assessed. After oral administration of different doses of <em>Radix Isatidis</em> extract (0.1,0.3,1 g/kg) to male rats, showed dose-dependent increases in R,S-goitrin's C_max and AUC, with bioavailability at 56.33 %. No gender differences in pharmacokinetics (PK) were observed. Compared with the monomer R,S-goitrin (0.03 mg/kg), it was observed higher <em>in vivo</em> exposure AUC_(0-t) and peak concentration C_max of R,S-goitrin after dosing of the Radix Isatidis extract with equal dosage of R,S-goitrin. R,S-goitrin was widely distributed in immune organs (adrenal glands, thymus, lymph nodes), liver, spleen, and gastrointestinal tract after oral administration of <em>Radix Isatidis</em> extract (0.1 g/kg) in rats. R,S-goitrin was primarily excreted <em>via</em> urine, accounting for 56 % of the administered dose, with plasma protein binding ranging from 13 % to 16.4 % across different species. These findings provide data to support <em>Radix Isatidis</em> clinical use in antibacterial, anti-inflammatory, anticancer therapies, and formulation development.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106514"},"PeriodicalIF":2.5,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenoxybiphenyl derivatives from an endophyte, Neohendersonia kickxii","authors":"Reo Takahashi ,&nbsp;Zulfikar ,&nbsp;Mayuka Hakozaki ,&nbsp;Yuko Kanno ,&nbsp;Shota Uesugi ,&nbsp;Takuya Koseki ,&nbsp;Yoshihito Shiono","doi":"10.1016/j.fitote.2025.106518","DOIUrl":"10.1016/j.fitote.2025.106518","url":null,"abstract":"<div><div>Three undescribed phenoxybiphenyl, neohenbiphenylols A (<strong>1</strong>), B (<strong>2</strong>) and C (<strong>3</strong>) along with known of diphenyl ethers <strong>4</strong>, and <strong>5</strong>, and dibenzofuran <strong>6</strong> were isolated from the endophyte <em>Neohendersonia kickxii</em> RT-749. The structures of these undescribed compounds, including their absolute configurations, were elucidated by analyses of NMR, UV, IR, HRESITOFMS, and ECD data. Compounds <strong>1</strong>–<strong>3</strong> showed cytotoxicity against human promyelocytic leukemia (HL60) cells with IC₅₀ values of 3.7, 4.3 and 7.3 μM, respectively, and antimicrobial activity against <em>Bacillus subtilis</em> with an MIC value of 25 <em>μ</em>gmL^-1.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106518"},"PeriodicalIF":2.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shuteria involucrata alleviates OVA-induced asthma through the TLR4/NF-κB pathway","authors":"Lue Bao ,&nbsp;Lisha Wei ,&nbsp;Xiaohong Li ,&nbsp;Xiaoyun Jiang ,&nbsp;Rong Duan ,&nbsp;Lingrui Zhou ,&nbsp;Lu Liu ,&nbsp;Wenhong Tan ,&nbsp;Feng Huang ,&nbsp;Zhuya Yang","doi":"10.1016/j.fitote.2025.106520","DOIUrl":"10.1016/j.fitote.2025.106520","url":null,"abstract":"<div><div>The roots of <em>Shuteria involucrata</em> (Wall.) Wight &amp; Arn., known as “Tong-qian-ma-huang” in <em>Dai</em> folk medicine in China, are renowned for their efficacy in treating asthma. Despite the use of <em>S. involucrata</em> has been reported as ameliorating respiratory diseases, its mechanism of action remains unclear. This work described the effect of ethyl acetate extract of <em>S. involucrate</em> (SSE) on allergic asthma and its potential mechanism of action was assessed. Ovalbumin was used <em>in vivo</em> to establish a BALB/c asthma mouse model. Lipopolysaccharide was used <em>in vitro</em> to stimulate the human macrophage cell line THP-1 to establish an inflammatory model. <em>In vivo</em> and <em>in vitro</em> experiments showed that SSE effectively ameliorated pathological symptoms and attenuated airway inflammation and cellular inflammation in allergic asthma mice, with a mechanism of action potentially associated to the inhibition of the TLR4/NF-κB signaling pathway.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106520"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of anti-arthritic and anti-inflammatory potential of Ajugarin-I from Ajuga Bracteosa wall ex. Benth against Freund's complete adjuvant induced arthritis","authors":"Fatima Zafar ,&nbsp;Hammad Ismail ,&nbsp;Ihsan-ul Haq","doi":"10.1016/j.fitote.2025.106517","DOIUrl":"10.1016/j.fitote.2025.106517","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is a prolonged autoimmune disease that targets the lining of small joints, causing inflammation and destruction of bone and cartilage. Anti-inflammatory drugs are available but offer short-term relief with adverse side effects. The present research was planned to study the in-vivo antiarthritic potential of ajugarin-I, sequestered from the <em>Ajuga bracteosa</em>. In-vivo studies include anti-inflammatory, analgesic, antidepressant, hematology profile, histopathological, radiological, and antioxidant analysis. The in-vivo results revealed that a high dose (HD, 60 mg/kg) of ajugarin-I significantly decreased the inflammatory paw edema (40 ± 0.04 %). Ajugarin-I also showed excellent analgesic behavior with 89 ± 0.55 % and 85 ± 0.55 % increments in pain threshold induced by cold and hot stimuli. Whereas ajugarin-I showed 95.1 ± 0.04 % antidepressant activity. Moreover, it showed strong antioxidant potential by raising the level of catalase, glutathione-S-transferase, and reducing glutathione while decreasing MDA levels in the liver, kidney, brain, paw, and spleen. Reduced serum levels of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) (with 67.5 ± 3.53 %, 83.5 ± 3.53 % and 85 ± 7.07 % respectively), were observed with HD of ajugarin-I. Furthermore, its 60 mg/kg dose restored hematological abnormalities by raising the Hb level (85 ± 0.7 %) RBCs (91 ± 0.5 %), MCV (83 ± 2 %), platelet count (83 ± 0.5 %) and lowered the WBC (68 ± 0.35 %) level. Abnormalities in histoarchitecture of paw, liver, and kidney were curtailed by HD of ajugarin-I demonstrating its regenerative properties. Radiological analysis of the left hind paw showed that ajugarin-I lessened the inflammation in articular areas. These findings will guide a novel area of research for exploiting ajugarin-I as novel anti-arthritic and anti-inflammatory agent.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106517"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eight new C21-steroidal glycosides from the roots of Cynanchum auriculatum","authors":"Ya-Yu Wu ,&nbsp;Lan-Wen Gao ,&nbsp;Yu-Jun Tang ,&nbsp;Bo Hu ,&nbsp;Ya-Lin Hu ,&nbsp;Yu-Bo Zhang ,&nbsp;Guang-Xiong Zhou","doi":"10.1016/j.fitote.2025.106519","DOIUrl":"10.1016/j.fitote.2025.106519","url":null,"abstract":"<div><div>Eight new C₂₁-steroidal glycosides (<strong>1</strong>–<strong>8</strong>) and fourteen known analogs (<strong>9</strong>–<strong>22</strong>) were isolated from the roots of <em>Cynanchum auriculatum</em> Royle ex Wight (Asclepiadaceae). The structures of the new compounds were elucidated on the basis of their IR, HR-ESI-MS, and NMR data. The cytotoxicities of compounds <strong>1</strong>–<strong>22</strong> were evaluated against two prominent human cancer cell lines, SW480 and MDA-MB-231, leading to preliminary structure-activity relationships (SARs). Notably, compounds <strong>1</strong>, <strong>5</strong>, <strong>8</strong>, <strong>10</strong>, and <strong>22</strong> exhibited significant cytotoxic activity against both cell lines, with IC₅₀ values ranging from 8.76 ± 1.36 to 17.90 ± 1.77 μM for SW480 and from 10.97 ± 1.73 to 12.07 ± 1.13 μM for MDA-MB-231.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106519"},"PeriodicalIF":2.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-diabetic sesquiterpenoids from the heartwood of Entandrophragma cylindricum","authors":"Hong-Yan Mu ,&nbsp;Kai-Wen Kang ,&nbsp;Yan Zhang ,&nbsp;Ju-Cai Xu ,&nbsp;Dong-Li Li ,&nbsp;Jing-Wei Jin ,&nbsp;Jia-Le Gan ,&nbsp;Hong-Guang Li ,&nbsp;Li-She Gan","doi":"10.1016/j.fitote.2025.106515","DOIUrl":"10.1016/j.fitote.2025.106515","url":null,"abstract":"<div><div>(<img>)-(7<em>S</em>,8<em>S</em>)-10,11-dihydro-curcumene-3,8-diol (<strong>1</strong>) and one new cadinane-type sesquiterpenoid, (<img>)-(1<em>R</em>,4<em>S</em>,6<em>S</em>,7<em>S</em>,10<em>R</em>)-cadinan-1,4-diol (<strong>2</strong>), together with six known ones (<strong>3–8</strong>), were isolated from 95 % ethanol extract of the heartwood of <em>Entandrophragma cylindricum</em>. Structures of the new compounds were elucidated on the basis of detailed spectroscopic analysis and quantum chemical calculations, including GIAO calculation of NMR chemical shifts and TDDFT calculation of ECD spectrum, which verified the relative and absolute configurations on the flexible side chain of <strong>1</strong>, respectively. All sesquiterpenoids were evaluated for their potential to enhance glucose uptake in C2C12 myotubes. Compound <strong>2</strong> (20 μM) significantly increased glucose uptake to the same level as that of metformin (2 mM). Western blot analysis on AMPKα, GLUT4, and ACC1 revealed that compound <strong>2</strong> activated the AMPK-GLUT4 signaling pathway in C2C12 myotubes.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106515"},"PeriodicalIF":2.5,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cassane butenolides from the fruits of Caesalpinia pulcherrima","authors":"Huiping Xie ,&nbsp;Ting Long ,&nbsp;Yongchun Gao ,&nbsp;Dan Huang ,&nbsp;Liqin Wang","doi":"10.1016/j.fitote.2025.106500","DOIUrl":"10.1016/j.fitote.2025.106500","url":null,"abstract":"<div><div>Seven undescribed cassane diterpenes, caeslpulrins A-G (<strong>1, 3, 4,</strong> and <strong>6–9</strong>), the C-12 ethoxy derivatives of caeslpulrins A and C (<strong>2</strong> and <strong>5</strong>), along with five artificial or known cassane diterpenes (<strong>10</strong>–<strong>14</strong>) and eight known sesquiterpenoids (<strong>15</strong>–<strong>22</strong>) were isolated from the fruits of <em>Caesalpinia pulcherrima</em>. Their structures were elucidated by extensive spectroscopic analyses, including HR-ESI-MS, 1D and 2D NMR, and electronic circular dichroism (ECD) calculations. Caeslpulrins A-F were identified as a group of rare cassane butenolides possessing a C-3 benzoyloxy unit. This is the first report of a series of such cassane diterpenes from a <em>Caesalpinia</em> plants<em>.</em> Compounds <strong>1–7</strong> were evaluated their inhibitory effects on NO production in the LPS-induced RAW 264.7 macrophages, and their cytotoxicities against several human tumor cell lines using MTS method.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106500"},"PeriodicalIF":2.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ten-membered lactones with antifungal activity against the postharvest pathogens from Penicillium restrictum","authors":"Jing Sun ,&nbsp;Feng-Shuang Wei ,&nbsp;Tong-De Zhao ,&nbsp;Rong-Fei Ma ,&nbsp;Xiao-Xiao Hu ,&nbsp;Heng-Xiang Li ,&nbsp;Chao Liu ,&nbsp;Ju-Cheng Zhang ,&nbsp;Xue-Qiong Yang ,&nbsp;Ya-Bin Yang ,&nbsp;Zhong-Tao Ding","doi":"10.1016/j.fitote.2025.106495","DOIUrl":"10.1016/j.fitote.2025.106495","url":null,"abstract":"<div><div>Two undescribed ten-membered lactones, named penicirestricols A (<strong>1</strong>) and B (<strong>2</strong>), and two unprecedented ten-membered lactone-adenine hybrids, penicirestricols C (<strong>3</strong>) and D (<strong>4</strong>), along with four known compounds, decarestrictines B (<strong>5</strong>), A₁ (<strong>6</strong>), F (<strong>7</strong>), and I (<strong>8</strong>) were isolated from the endophytic fungus <em>Penicillium restrictum</em>. The structures were determined by 1D, 2D NMR techniques, mass spectrometry, ECD, and NMR calculations. Compounds <strong>3</strong>, <strong>7</strong>, and <strong>8</strong> from <em>P. restrictum</em> showed significant antifungal activities against postharvest pathogens <em>Fusarium asiaticum</em> and <em>Alternaria tenuissima</em> with MICs ≤8 μg/mL. Thus, the compounds isolated from <em>P. restrictum</em> have the potentiality to be developed as the novel antifungal agents.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"183 ","pages":"Article 106495"},"PeriodicalIF":2.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}