Fitoterapia最新文献

{"title":"Investigation of anti-hepatocellular carcinoma active components and mechanism of Prinsepia utilis seed residue via spectrum-effect relationship integrated with network pharmacology","authors":"Hongchao Deng ,&nbsp;Yixin Liu ,&nbsp;Chen Zhang ,&nbsp;Xin Pang ,&nbsp;Zitao Guo ,&nbsp;Moying Li ,&nbsp;Liang Zhang","doi":"10.1016/j.fitote.2025.106834","DOIUrl":"10.1016/j.fitote.2025.106834","url":null,"abstract":"<div><div>In recent years, the pharmacological activities and potential applications of <em>Prinsepia utilis</em> have garnered significant attention. However, most studies have focused on <em>Prinsepia utilis</em> oil, whereas research on its seed residue remains limited. In this study, the anti-hepatocellular carcinoma activity of <em>Prinsepia utilis</em> seed residue was firstly investigated using an integrated strategy of spectrum-effect relationship analysis and network pharmacology. The chemical fingerprint of <em>Prinsepia utilis</em> seed residue was established via UPLC-Q-TOF-MS. Proliferation inhibition (CCK-8 assay) and migration capacity (scratch assay) were evaluated in HepG₂/HSF cells. Ten potential bioactive components including five flavonoid glycosides and five diterpenoid glycosides were identified via spectrum-effect relationship analysis. Network pharmacology revealed 46 anti-tumor and 57 anti-scar hyperplasia targets. Key pathways included cancer, lipid and atherosclerosis, relaxin signaling, and IL-17 signaling. Core targets were prioritized, and molecular docking confirmed favorable binding affinities between bioactive aglycones and these targets. The flavonoid glycosides and diterpenoid glycosides in <em>Prinsepia utilis</em> seed residue exert anti-hepatocellular carcinoma activity primarily by targeting MAPK14, IL-6, STAT3, HRAS, GNAS, CXCL8, and SRC, thus establishing a scientific rationale for their development as potential therapeutic agents.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106834"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of dopamine D1 receptor antagonists from Stephania epigaea using label-free cell phenotypic assays","authors":"Hongjian Kang ,&nbsp;Hongming Tang ,&nbsp;Jixia Wang ,&nbsp;Yanfang Liu ,&nbsp;Feifei Huang ,&nbsp;Han Zhou ,&nbsp;Jiapeng Guo ,&nbsp;Lai Wei ,&nbsp;Xinmiao Liang ,&nbsp;Xingya Xue","doi":"10.1016/j.fitote.2025.106832","DOIUrl":"10.1016/j.fitote.2025.106832","url":null,"abstract":"<div><div>The traditional Chinese medicine <em>Stephania epigaea</em> (<em>S. epigaea</em>) has been used for medicinal purposes, including the treatment of abdominal pain and malaria. Increasing evidence suggests a relationship between pain relief, gut regulation, and the dopaminergic system, particularly the dopamine D1 receptor (D1R). However, it remains unclear whether and how active components of <em>S. epigaea</em> exert their effects through dopamine receptors. This study aims to discover active alkaloids targeting D1R from <em>S. epigaea</em>, evaluate their potencies, and analyze their structure-activity relationships (SARs). Twenty-four alkaloids were screened on an HEK293-D1R cell model using label-free cell phenotypic assays, and six were identified for the first time as exhibiting D1R antagonistic activity, including capaurimine, (−)-discretine, (+)-corydaline, <em>N</em>-methyllaurotetanine, (+)-isocorydine and <em>O</em>-methylbulbocapnine. Among these, protoberberine-type alkaloids capaurimine and (−)-discretine were found to be potent D1R antagonists, with IC₅₀ values of 0.16 ± 0.02 μM and 0.25 ± 0.01 μM, respectively. The aporphine-type alkaloid <em>N</em>-methyllaurotetanine also exhibited micromolar-level D1R antagonistic activity (IC₅₀ = 1.65 ± 0.20 μM). Their kinetic binding profiles were characterized using co-stimulation assay, confirming them as competitive D1R antagonists. Additionally, these compounds were docked with the crystal structure of human D1R, allowing for an analysis their SARs. These findings provide potential mechanisms underlying the analgesic and gastrointestinal effects of <em>S. epigaea</em> and offer valuable information for D1R antagonist drug design.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106832"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo and in silico investigations of flavonoids from Rhamnus alaternus L. in mitigating ethanol-induced oxidative stress and inflammation","authors":"Tahsine Kosksi ,&nbsp;Mabrouk Horchani ,&nbsp;Salima Selmi ,&nbsp;Nawres Debbabi ,&nbsp;Arem Selmi ,&nbsp;Marwa Rejeb ,&nbsp;Sihem Safta Skhiri ,&nbsp;Hichem Ben Jannet ,&nbsp;Leila Chekir Ghedira ,&nbsp;Ines Bouhlel Chatti","doi":"10.1016/j.fitote.2025.106835","DOIUrl":"10.1016/j.fitote.2025.106835","url":null,"abstract":"<div><div>To investigate the protective effects of <em>Rhamnus alaternus</em> L. against ethanol-induced toxicity, female Wistar rats were divided into 3 groups of 8 rats: control, ethanol (4 g/kg - 40 %), and ethanol with total oligomer flavonoids (TOF) (5 mg/kg). Various parameters, including biochemical and oxidative stress markers, histopathological damage, reactive oxygen species (ROS), nitric oxide (NO) and lysosomal activity in peritoneal macrophages, and cytokine levels, were evaluated after 4 weeks of treatment. Our study demonstrated that ethanol induced a considerable disruption of hematological indices, an increased level of biochemical markers, and MDA level with decreased antioxidant enzyme activities. Furthermore, an increase in ROS, NO levels, lysosomal activity and a significant increase in cytokines levels, indicated inflammation. TOF, however, restored normal levels of hematological indices, biochemical, antioxidant enzymes, and cytokines, and improved histological anomalies. TOF was found to protect rats from ethanol-induced damage owing to its antioxidant and anti-inflammatory properties. In addition, molecular docking simulations of the identified phytocompounds against ‘superoxide dismutase (pdb:4A7G)’ and ‘glutathione peroxidase 1 (pdb:2F8A)’ were performed. Significant results are obtained especially with ‘Kaempferol-3-O-rutinoside’.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106835"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pomegranate (Punica granatum L.): A narrative review of its protective role against nephrotoxicity","authors":"Zahra Yazdanpanah ,&nbsp;Ali Sirjani ,&nbsp;Elnaz Vafadar Moradi ,&nbsp;Behrang Rezvani Kakhki ,&nbsp;Jalileh Jalali ,&nbsp;Mahboobeh Ghasemzadeh Rahbardar","doi":"10.1016/j.fitote.2025.106827","DOIUrl":"10.1016/j.fitote.2025.106827","url":null,"abstract":"<div><h3>Background</h3><div>Pomegranate (<em>Punica granatum</em> L.) has been traditionally used in various cultures for its health benefits, particularly in promoting kidney health. Its rich composition of bioactive compounds, including polyphenols and flavonoids, has drawn attention for their potential protective effects against nephrotoxicity, a condition characterized by kidney damage due to various toxic agents.</div></div><div><h3>Aim of the study</h3><div>This review aims to evaluate the efficacy of pomegranate and its active constituents in mitigating nephrotoxicity and promoting kidney health.</div></div><div><h3>Methods</h3><div>A literature review was conducted from 2010 to 2025, encompassing relevant <em>in vitro</em> and <em>in vivo</em> studies sourced from databases such as Scopus, Google Scholar, Web of Science, and PubMed. The focus was on the effects of pomegranate on nephrotoxicity and its underlying mechanisms.</div></div><div><h3>Results</h3><div>The nephroprotective effects of pomegranate are attributed to its bioactive compounds, including ellagic acid, gallic acid, and punicalagin, which exhibit robust antioxidant properties and reduce inflammation, apoptosis, and fibrosis. These compounds suppress the TGF-β1/Smad pathway while enhancing protective regulators (SIRT1, SIRT6, TUG1, and Nrf2), contributing to antioxidant defense and cellular homeostasis. Furthermore, pomegranate mitigates nephrotoxicity by decreasing markers like MCP-1, NF-κB, LDH, HIF-1α, KIM-1, and NGAL, and it modulates renal transport proteins (OAT1 and OAT3). By preserving glomerular filtration rate, alleviating tubular damage, and promoting renal repair mechanisms, pomegranate demonstrates its protective actions against nephrotoxicity.</div></div><div><h3>Conclusion</h3><div>Pomegranate shows multifaceted nephroprotective properties through modulation of oxidative stress, inflammation, fibrosis, and apoptosis-related signaling pathways. However, further research is essential to confirm these findings, explore clinical applications, and evaluate safety profiles and potential interactions with other medications.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106827"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Justicia procumbens ameliorates aortic aging via ferroptosis regulation in senescent mice and vascular smooth muscle cells","authors":"Jong Min Kim ,&nbsp;Hyun-Jin Na ,&nbsp;Yiseul Kim,&nbsp;Sang Hee Lee,&nbsp;Chang Hwa Jung,&nbsp;Mi Jeong Sung","doi":"10.1016/j.fitote.2025.106829","DOIUrl":"10.1016/j.fitote.2025.106829","url":null,"abstract":"<div><div>Vascular aging involves structural remodeling, oxidative stress, lipid peroxidation, and phenotypic switching of vascular smooth muscle cells (VSMCs), all contributing to age-related cardiovascular diseases. Ferroptosis, a regulated iron-dependent cell death, is a key mechanism of vascular aging. <em>Justicia procumbens</em> (JP), a medicinal plant rich in lignans and flavonoids, exhibits antioxidant properties; however, its effects on vascular aging are unclear. This study investigated the vascular protective effects of JP using senescent mice and Erastin-induced A7r5 smooth muscle cells. In aged mice, JP suppressed aortic thickening, reduced serum iron levels, and normalized transferrin receptor and apoptosis-inducing factor mitochondria-associated 2 expression. JP attenuated ferroptosis by decreasing the levels of 4-hydroxynonenal, prostaglandin-endoperoxide synthase 2, and acyl-CoA synthetase long-chain family member 1, and restoring the levels of solute carrier family 7 member 11 and glutathione peroxidase 4. Furthermore, JP preserved the expression of the VSMC contractile markers (alpha-smooth muscle actin, smooth muscle 22 alpha, and calponin), reduced the level of the synthetic marker osteopontin, and enhanced the activity of antioxidant enzymes (superoxide dismutase, catalase, and NADPH oxidase 1). In vitro, JP protected A7r5 cells from ferroptosis-induced cytotoxicity, promoted proliferation and migration, and restored ferroptosis and antioxidant markers. These results suggest that JP mitigates vascular aging by modulating ferroptosis, iron homeostasis, oxidative stress, and VSMC phenotype. JP may serve as a promising natural therapeutic agent for preventing age-related vascular degeneration and promoting vascular health.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106829"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted isolation of new antibacterial sesquiterpene coumarins from ammoniacum (Ferula communis L.)","authors":"Capucine Braillon ,&nbsp;Elora Aubert ,&nbsp;Régine Janet-Bintz ,&nbsp;Megi Gjikolaj ,&nbsp;Rania Hamidi ,&nbsp;Léa Blondé ,&nbsp;Jean Peluso ,&nbsp;Pascal Villa ,&nbsp;Véronique Pitchon ,&nbsp;Pierre Fechter ,&nbsp;Catherine Vonthron-Sénécheau ,&nbsp;Sergio Ortiz","doi":"10.1016/j.fitote.2025.106833","DOIUrl":"10.1016/j.fitote.2025.106833","url":null,"abstract":"<div><div>The use of plant-based remedies in traditional medecine to combat bacterial infections has been scientifically studied. As part of an ongoing research for the discovery of new therapeutic options to treat bacterial infections, Ammoniacum gum resin from <em>Ferula communis</em> L. roots was explored. Ammoniacum appeared in many traditional formulations of Arab, Indian and Western medicines and its use has been reported throughout the centuries. This present study aimed to further explore this gum-resin using a molecular-networking-guided method for the accelerated discovery of new antibacterial compounds. HPLC-PDA-HRMS/MS molecular-networking-based dereplication strategy highlighted the presence of known sesquiterpene coumarins among potential new derivatives. By comparison of MS/MS fragmentation spectra between known and unknown compounds, a hydroxycinnamic sesquiterpene coumarin structure was predicted as a potential new compound. Mass-guided isolation followed by structural elucidation allowed us to corroborate the predicted structure of this new derivative identified as <em>o</em>-coumaric-<em>ω</em>-hydroxyferulenol (<strong>10</strong>). The new derivative showed MIC₉₀ values of 1.84–6.17 μM against cutaneous Gram-positive bacteria with a bactericidal effect measured by MBC/MCI ratio evaluation and flow cytometry methodology. Furthermore, the new compound showed no <em>in vitro</em> cytotoxicity against eukaryotic cells. This study confirms the interest to analyse with modern techniques ancient traditional remedies in the exploration of new bioactive compounds with therapeutical potential.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106833"},"PeriodicalIF":2.6,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-targeted metabolomic analysis of Xueshuantong injection in patients with STEMI","authors":"Xiaowan Han ,&nbsp;Wang Lei ,&nbsp;Li Chunyan,&nbsp;Xie Jing,&nbsp;Ren Xiaoxia,&nbsp;Xuan Changbo,&nbsp;Wang Zichen,&nbsp;Wang Nina,&nbsp;Peng Wenhua,&nbsp;Guozhong Pan","doi":"10.1016/j.fitote.2025.106802","DOIUrl":"10.1016/j.fitote.2025.106802","url":null,"abstract":"<div><div>Xueshuantong injection (XST) is a widely used traditional Chinese medicine for the treatment of cardiovascular diseases, particularly in the context of acute ST-segment elevation myocardial infarction (STEMI). This study aimed to explore the therapeutic mechanism of XST on STEMI by conducting a metabolomics analysis of plasma samples from 23 patients with STEMI. The patients were divided into a control group (13 patients) and an experimental group (10 patients), both undergoing emergency coronary intervention (PCI) and standardized drug therapy. The experimental group received XST for 7 days. The results demonstrated that XST can improve ventricular remodeling by modulating 71 different metabolites. Metabolic pathway enrichment analysis revealed significant enrichment in several pathways, including caffeine metabolism, phenylalanine, tyrosine, and tryptophan biosynthesis, as well as starch and sucrose metabolism. Further analysis suggested that the protective effect of XST on STEMI patients might be attributed to the regulation of the PI3K-Akt signaling pathway, MAPK signaling pathway, Ras signaling pathway, and lipid metabolism and atherosclerosis. Molecular docking technology confirmed that the main component of XST has high binding activity and stable docking conformation with key targets for treating STEMI. These findings contribute to a better understanding of the therapeutic potential of XST for treating STEMI.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106802"},"PeriodicalIF":2.6,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tanshinone in acne therapeutics: Integrating molecular mechanisms, drug development, and multimodal treatment strategies","authors":"Tengyue Zhang ,&nbsp;Zhijie Gao ,&nbsp;Yanhua Wang","doi":"10.1016/j.fitote.2025.106822","DOIUrl":"10.1016/j.fitote.2025.106822","url":null,"abstract":"<div><div>Tanshinones (TS), bioactive lipophilic diterpenoids derived from <em>Salvia miltiorrhiza</em>(SM), demonstrate significant therapeutic promise for acne vulgaris (AV)—a multifactorial dermatosis characterized by P.acnes colonization, aberrant follicular keratinization, and immune-inflammatory cascades exacerbated by endocrine dysregulation and psychosocial stressors. This comprehensive review synthesizes recent advances elucidating TS's tripartite mechanistic framework: (1) Direct antibacterial activity against <em>P. acnes</em> and multidrug-resistant <em>Staphylococcus aureus</em>, disrupting biofilm formation and quorum sensing; (2) Immunomodulation via suppression of NLRP3 inflammasomes, inhibition of MAPK/NF-κB signaling pathways, and downregulation of pro-inflammatory cytokines; (3) Sebostatic and antioxidant effects through modulation of SREBP-1/FASN-mediated lipid biosynthesis, Nrf2/ARE pathway activation, SOD/COX-2 regulation, and potent ROS scavenging. Innovations in pharmaceutical engineering—including carrier-free self-assembled nanoparticles, autolytic microneedle arrays, photoactivated nanoemulsions, and chitosan-based nanoencapsulated gels—have markedly enhanced TS bioavailability, dermal retention, and pilosebaceous targeting. Clinical evaluations confirm TS formulations significantly reduce inflammatory lesion counts, acne recurrence rates, serum testosterone, and oxidative stress biomarkers. Synergistic regimens combining TS with photodynamic therapy, intense pulsed light, or conventional agents demonstrate enhanced anti-acne efficacy while minimizing cutaneous irritation. Despite promising outcomes, translational challenges persist, including pharmacokinetic limitations, clinical validation gaps in long-term safety, and scale-up complexities of advanced delivery systems. Future research should prioritize randomized multicenter trials, predictive ex vivo models, and hybrid delivery platforms to accelerate evidence-based integration into dermatological practice.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106822"},"PeriodicalIF":2.6,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three new polyketones from mangrove endophytic fungus aspergillus sp. HMGh1–1 and their insecticidal activity","authors":"Wenjun Li,&nbsp;Meijing Tan,&nbsp;Shichao Ju,&nbsp;Fan He,&nbsp;Jiao Wu,&nbsp;Youquan Wei,&nbsp;Jinyu Wen,&nbsp;Baoliang Li,&nbsp;Xishan Huang","doi":"10.1016/j.fitote.2025.106803","DOIUrl":"10.1016/j.fitote.2025.106803","url":null,"abstract":"<div><div>A new phenylacetic acid, methoxy-graphislactones C (<strong>1</strong>), a new anthraquinone (1′E)-6,8-di-<em>O</em>-methyl averanti (<strong>2</strong>), a new phenanthrene derivative, 12-methyl entonaemin A (<strong>3</strong>) and five previously reported compounds (<strong>4</strong>–<strong>8</strong>) were isolated from the fermented extracts of the mangrove endophytic fungus <em>Aspergillus</em> sp. HMGh1–1. The structures and absolute configurations of the new compounds were determined by spectroscopic techniques (high-resolution mass spectrometry, NMR), ECD calculations, and single-crystal X-ray analysis. Compounds <strong>1</strong>–<strong>8</strong> were screened for insecticidal activity against <em>Diaphorina citri</em> Kuwayama, and compound <strong>3</strong> exhibited significant insecticidal activity, with a corrected efficacy of 90.97 %.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106803"},"PeriodicalIF":2.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stellarine A attenuates anxiety in rats through ERK/CREB/BDNF signaling: A 1HNMR metabolomics study revealing metabolic pathways and biomarkers","authors":"Meng Zhan ,&nbsp;Ying Zhang ,&nbsp;Xiyan Mo ,&nbsp;Miao Ma ,&nbsp;Yangkun Zhou ,&nbsp;Tingting Li ,&nbsp;Hongfeng Quan ,&nbsp;Lin Dong","doi":"10.1016/j.fitote.2025.106820","DOIUrl":"10.1016/j.fitote.2025.106820","url":null,"abstract":"<div><div>Anxiety is one of the most prevalent mental health issues. Stellariae Radix, a specialty medicinal material from Ningxia, is known for its ability to clear defunct heat. Research group prior research demonstrated that the ethanol extract of Stellariae Radix possesses a significant anxiolytic effect. We have separated and identified the β-carboline alkaloid compounds from Stellariae Radix. The anxiolytic properties of Stellarine A were assessed in rats by conducting behavioral assays and examining pathological histology. Concurrently, ELISA was utilized to detect changes in neurotransmitter and hormone levels, and molecular biology techniques were employed to explore its mechanisms of action. ¹HNMR analysis was conducted to examine metabolic changes in rats, aiming to identify biomarkers and metabolic pathways. The pharmacodynamic data demonstrated that Stellarine A can significantly improve rat anxiety behavior. Moreover, Stellarine A significantly ameliorates the reduction and disarray of neurons. Immunohistochemical and molecular biology techniques reveal that Stellarine A upregulates the expression of CB1 and CB2, enhances ERK, CREB, and BDNF mRNA and protein expression. ¹HNMR analysis identified 28 endogenous differential metabolites associated with the anxiety model, and Stellarine A significantly modulates 21 endogenous metabolites, thereby improving anxiety symptoms by regulating multiple metabolites. The component Stellarine A in Stellariae Radix possesses anxiolytic potential, which may exert its effects by activating the ERK/CREB/BDNF pathway. The anxiolytic action of Stellarine A is associated with metabolic pathways such as amino acid metabolism, sugar metabolism, and lipids. Therefore, Stellarine A shows promise in the development of therapeutic drugs for anxiety disorders.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106820"},"PeriodicalIF":2.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144862230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}