Fitoterapia最新文献_第9页

Zanthoxylum armatum DC. Extract induced liver injury via DNA double-strand breaks-mediated cellular senescence pathway 花椒花椒提取物通过DNA双链断裂介导的细胞衰老途径诱导肝损伤

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-21 DOI: 10.1016/j.fitote.2025.106841

Jiayu Wen , Qiwen Xiang , Jiafu Guo , Xinyi Zhou , Yan Chen , Yan Huang , Chaolong Rao

{"title":"Zanthoxylum armatum DC. Extract induced liver injury via DNA double-strand breaks-mediated cellular senescence pathway","authors":"Jiayu Wen , Qiwen Xiang , Jiafu Guo , Xinyi Zhou , Yan Chen , Yan Huang , Chaolong Rao","doi":"10.1016/j.fitote.2025.106841","DOIUrl":"10.1016/j.fitote.2025.106841","url":null,"abstract":"<div><h3>Aim of the study</h3><div>To comprehensively explore the impact of <em>Zanthoxylum armatum DC</em>. (ZADC) on liver injury and disclose its mechanistic underpinnings, thereby laying a scientific foundation for the secure application of ZADC.</div></div><div><h3>Materials and methods</h3><div>The ethyl acetate extract of ZADC (ZADC-EA) was subjected to analysis via Q-Orbitrap LC-MS/MS. The damage effect of ZADC-EA was appraised by determining cell viability, liver function index, and inflammation levels. Western blot, RT-qPCR, flow cytometry, and immunofluorescence were employed to detect DNA double-strand breaks (DSBs) and cellular senescence, with the aim of unraveling the mechanism of liver injury instigated by ZADC-EA.</div></div><div><h3>Results</h3><div>Chemical composition analysis unveiled the presence of flavonoids, organic acids, and coumarins in ZADC-EA. Exposure to ZADC-EA caused a decrease in the viability of HepG2 cells and an elevation in the levels of liver injury markers and inflammatory factors. Moreover, treatment with ZADC-EA triggered DSBs and activated the p53-p21 pathway, culminating in the induction of cellular senescence.</div></div><div><h3>Conclusions</h3><div>The research provides a novel perspective for the safety assessment of ZADC. It has been demonstrated that ZADC-EA can precipitate DSBs and induce cellular senescence by activating the p53-p21 signaling pathway, thereby giving rise to liver damage.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106841"},"PeriodicalIF":2.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yanghuo Sanqi capsule against Alzheimer's disease by upregulating lipid metabolism: Network pharmacology and multi-omics analysis 养火三七胶囊通过上调脂质代谢抗阿尔茨海默病：网络药理学和多组学分析

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-21 DOI: 10.1016/j.fitote.2025.106837

Jiali Rao , Haoxue Zheng , Min Lu , Jingxu Chen , Chengcheng Wang , Yan Cao

{"title":"Yanghuo Sanqi capsule against Alzheimer's disease by upregulating lipid metabolism: Network pharmacology and multi-omics analysis","authors":"Jiali Rao , Haoxue Zheng , Min Lu , Jingxu Chen , Chengcheng Wang , Yan Cao","doi":"10.1016/j.fitote.2025.106837","DOIUrl":"10.1016/j.fitote.2025.106837","url":null,"abstract":"<div><div>Yanghuo Sanqi capsule (YSC) consists of Yingyanghuo <em>(Epimedium brevicornu Maxim.</em>, YYH) and Sanqi (<em>Panax notoginseng</em> (Burk.) F.H.Chen, SQ), which is used to treat cardiovascular diseases in Traditional Chinese Medicine (TCM). Additionally, the accumulated evidence suggests that it has the potential to treat Alzheimer's disease (AD). This study tries to elucidate the mechanisms of YSC against AD by combining network pharmacology of absorbed components with multi-omics of <em>Caenorhabditis elegans</em> (<em>C. elegans</em>). Firstly, network pharmacology was used to predict the target of the YSC in the treatment of AD. Then, <em>Caenorhabditis elegans</em> (<em>C. elegans</em>) CL4176 and CL2355 were used to study the effect of YSC on AD, and the mechanism was studied through multi-omics. Finally, potential therapeutic targets and bioactivity ingredients were identified through validation experiments, molecular docking, and molecular dynamics simulation. The network pharmacology of 15 mice blood components suggested that YSC may intervene in AD by regulating lipid metabolism and amyloid precursor protein (APP). In addition, YSC can alleviate Aβ-induced toxicity in both muscle and nerve in <em>C. elegans</em>. Transcriptomic and metabolomic analysis in <em>C. elegans</em> showed that YSC protected against AD by increasing lipid metabolism, which can maintain lipid homeostasis inside and outside the cell and down-regulating APP to alleviate Aβ-induced toxicity. Anhydroicaritin (AHI) is an important bioactive ingredient involved in regulating APP error clipping and lipid metabolism in YSC. This study reveals the mechanism of YSC anti-AD and provides experimental support for expanding its clinical use.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106837"},"PeriodicalIF":2.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiproliferative withanolides from Jaborosa riojana 抗增殖的紫檀花内酯类物质

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-20 DOI: 10.1016/j.fitote.2025.106826

Virginia Lobatto , Carina N. Casero , Aday González-Bakker , José M. Padrón , Viviana E. Nicotra

引用次数: 0

New C-24 epimers of dammarane-type saponins from Gynostemma pentaphyllum and their absolute configurations 绞股蓝达玛烷型皂苷新的C-24外旋体及其绝对构型

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-20 DOI: 10.1016/j.fitote.2025.106825

Peng-Xin Lu , Gang Li , Hai-Zhen Liang, Xiao-Juan Chen, Guang Yang, Qi Li, Jie Zhang, Bai-Ping Ma

{"title":"New C-24 epimers of dammarane-type saponins from Gynostemma pentaphyllum and their absolute configurations","authors":"Peng-Xin Lu , Gang Li , Hai-Zhen Liang, Xiao-Juan Chen, Guang Yang, Qi Li, Jie Zhang, Bai-Ping Ma","doi":"10.1016/j.fitote.2025.106825","DOIUrl":"10.1016/j.fitote.2025.106825","url":null,"abstract":"<div><div>Seventeen new dammarane-type saponins, gypenosides FJ1 <strong>−</strong> FJ17 (<strong>1–17</strong>), and five known compounds (<strong>18–22</strong>) including nine pairs of C-24 epimers were isolated from <em>Gynostemma pentaphyllum</em>. Their structures were determined through comprehensive 1D and 2D NMR spectroscopic analyses and HR-ESI-MS data. The absolute configurations of five pairs of C-24 epimer compounds (<strong>1</strong>–<strong>10</strong>) were assigned via electronic circular dichroism (ECD) analyses. Interestingly, a detailed spectroscopic analysis indicated that the C-24 configuration of epimers was associated with the chemical shift difference value of H-22 (Δ<em>δ</em><sub>H-22</sub> > 0.5, 24<em>R</em>; Δ<em>δ</em><sub>H-22</sub> < 0.5, 24<em>S</em>), leading to a simple and convenient method for the rapid assignment of C-24 configuration in C-24 epimers of dammarane-type saponins. This rule enabled rapid C-24 configuration assignment for compounds <strong>11</strong>–<strong>22</strong>. Anti-hypoxic activity evaluation in PC12 cells under hypoxia demonstrated that compounds <strong>8, 9, 11, 13</strong> and <strong>16–20</strong> effectively protected PC12 cells from hypoxia-induced injury.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106825"},"PeriodicalIF":2.6,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lathyrane and jatrophane diterpenoids: Potential lipid-lowering agents from Euphorbia helioscopia L 大戟烯和麻风碱二萜：潜在的降脂剂

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-19 DOI: 10.1016/j.fitote.2025.106830

Sheng Chen , Wen-Zheng Zhao , Zhi-Yan Zhang , Hui-Lan Yue , Qiang-Qiang Shi

引用次数: 0

New polyhydroxy cucurbitane-type triterpenoids from the tubers of Hemsleya chinensis and their cytotoxic activities 中国海草块茎中新的多羟基葫芦烷型三萜及其细胞毒活性

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-19 DOI: 10.1016/j.fitote.2025.106840

Wan-Bi Feng , Ming-Shang Liu , Tong-Xin Zheng , Zi-Xin Lin , Hai-Wei Xu , Hai-Tao Cai , Yu-Bo Zhang , Jia-Lue Bai , Mao-Xun Yang , Hui Luo , Xiao-San Li

{"title":"New polyhydroxy cucurbitane-type triterpenoids from the tubers of Hemsleya chinensis and their cytotoxic activities","authors":"Wan-Bi Feng , Ming-Shang Liu , Tong-Xin Zheng , Zi-Xin Lin , Hai-Wei Xu , Hai-Tao Cai , Yu-Bo Zhang , Jia-Lue Bai , Mao-Xun Yang , Hui Luo , Xiao-San Li","doi":"10.1016/j.fitote.2025.106840","DOIUrl":"10.1016/j.fitote.2025.106840","url":null,"abstract":"<div><div>Phytochemical investigation of the 95 % ethanol extract of the tubers of <em>Hemsleya chinensis</em> led to the isolation of five previously undescribed cucurbitane-type triterpenoids (<strong>1–5</strong>, named hemslyencins G-K), along with five known analogues (<strong>6–10</strong>). The structures of these compounds were elucidated through comprehensive spectroscopic analysis (UV, IR, HR-ESI-MS, 1D and 2D NMR data). Compounds <strong>1</strong>, <strong>2</strong>, <strong>7</strong>, <strong>9</strong>, and <strong>10</strong> are characterized as 24‑oxygenated cucurbitane-type triterpenoids, were rarely found in the genus <em>Hemsleya</em>. The cytotoxic activities of <strong>1–10</strong> were evaluated against MCF-7, HCT-116, HeLa, and HepG2 cancer cell lines, in which compounds <strong>1</strong> and <strong>8–10</strong> exhibited significant cytotoxic activities with the value of 0.382–13.77 μM. Further mechanism studies displayed that compound <strong>1</strong> dose-dependently induced apoptosis and caused G0/G1 phase cell cycle arrest in MCF-7 cells<em>.</em></div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106840"},"PeriodicalIF":2.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secondary metabolites from the karst cave fungus Roussoella sp. LCP1-2 with proangiogenic activity 具有促血管生成活性的溶洞真菌Roussoella sp. LCP1-2次生代谢物

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-19 DOI: 10.1016/j.fitote.2025.106838

Caixia Wei , Jian Sun , Jingyuan Tian , Ting Shu , Rongxiang Wu , Yi Hao , Kexin Cheng , Cong Wang , Fandong Kong , Liman Zhou

{"title":"Secondary metabolites from the karst cave fungus Roussoella sp. LCP1-2 with proangiogenic activity","authors":"Caixia Wei , Jian Sun , Jingyuan Tian , Ting Shu , Rongxiang Wu , Yi Hao , Kexin Cheng , Cong Wang , Fandong Kong , Liman Zhou","doi":"10.1016/j.fitote.2025.106838","DOIUrl":"10.1016/j.fitote.2025.106838","url":null,"abstract":"<div><div>Six compounds were isolated from the fermentation broth of <em>Roussoella</em> sp. LCP1–2, including four new metabolites: papyracillic acid D (<strong>1</strong>), 7-<em>epi</em>-4-<em>epi</em>-papyracillic acid B (<strong>2</strong>), 4-<em>epi</em>-papyracillic acid B (<strong>3</strong>), and 3-hydroxyl-11α,18-Dihydroxy-7-oxo-13-<em>epi</em>-<em>ent</em>-pimara-8(9),15-diene (<strong>5</strong>), along with two known compounds, papyracillic acid B (<strong>4</strong>) and saccharonol A (<strong>6</strong>). Their planar structures were elucidated through comprehensive analysis of NMR and MS spectroscopic data. The structure of <strong>1</strong> was unambiguously confirmed by single-crystal X-ray diffraction analysis. The absolute configurations of compounds <strong>2</strong>, <strong>3</strong>, and <strong>5</strong> were established through a combination of experimental ECD spectroscopy and computational ECD calculations. Biological evaluation revealed that compound <strong>3</strong> exhibited moderate proangiogenic activity in a zebrafish model at a concentration of 40 μM.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106838"},"PeriodicalIF":2.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of lignans from leaves of Phyllanthus emblica L. and their antioxidant-antiinflammatory activity 余甘子叶中木脂素的发现及其抗氧化抗炎活性

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-19 DOI: 10.1016/j.fitote.2025.106831

Dian Luo , Min Yang , Yuan-Meng Li , Hong-Tao Zhu , Ping Zhao , Ying-Jun Zhang

引用次数: 0

Natural long-chain fatty acyl solamines from the leaves of Solanum bulbocastanum Dun. show antiseptic properties 从龙葵叶中提取的天然长链脂肪酸酰基茄胺。显示防腐性能

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-19 DOI: 10.1016/j.fitote.2025.106836

Alexander Sonntag , Karin Gorzolka , Franziska Schanbacher , Myong-Su Lenz , Fabian Herrmann , Anton M. Zürbig , Stefan Bereswill , Markus M. Heimesaat , Matthias F. Melzig , Alexander Weng

{"title":"Natural long-chain fatty acyl solamines from the leaves of Solanum bulbocastanum Dun. show antiseptic properties","authors":"Alexander Sonntag , Karin Gorzolka , Franziska Schanbacher , Myong-Su Lenz , Fabian Herrmann , Anton M. Zürbig , Stefan Bereswill , Markus M. Heimesaat , Matthias F. Melzig , Alexander Weng","doi":"10.1016/j.fitote.2025.106836","DOIUrl":"10.1016/j.fitote.2025.106836","url":null,"abstract":"<div><div>Long-chain fatty acyl solamines, recently identified in the leaves of <em>Solanum bulbocastanum</em> Dun., have been shown to confer resistance against plant pests such as the Colorado potato beetle and the phytopathogenic oomycete <em>Phytophthora infestans</em> (Mont.) de Bary. Owing to their structural resemblance to the antiseptic cetylpyridinium chloride, this study aimed to evaluate a fraction of a methanolic <em>S. bulbucastanum</em> leaf extract mainly containing long-chain fatty acyl solamines for potential antiseptic applications. Minimal inhibitory concentrations (MICs) were determined against eight clinically relevant human pathogens, and minimal bactericidal concentrations (MBCs) were assessed for two model bacteria. Atomic force microscopy and lactate dehydrogenase (LDH) release assays were employed to investigate the mechanism of action. Cytotoxicity was evaluated on normal human dermal fibroblasts and Vero E6 cells using MTT and LDH assays, as well as real-time cell analysis and live-cell imaging. The fraction exhibited antimicrobial activity against all tested pathogens (MICs of 128–256 μg/mL for the majority of the pathogens) and demonstrated bactericidal properties (MBCs of 256 μg/mL), likely mediated by nonspecific membrane disruption. In mammalian cell assays, the fraction mirrored the cytotoxic effect of cetylpyridinium chloride in MTT assay (Fibroblasts: IC<sub>50</sub> = 1.2 μg/mL, Vero E6: IC<sub>50</sub> = 2.2 μg/mL) and the profile in real time cell analysis, suggesting a comparable mode of action. These findings highlight long-chain fatty acyl solamines as promising candidates for the development as bio-based antiseptics, offering a potential green alternative to conventional agents.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106836"},"PeriodicalIF":2.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotransformation of oleanonic acid from jujube by food fermentation fungus Actinomucor elegans and the potential anti-neuroinflammatory properties of metabolites 食品发酵真菌对枣中齐墩果酸的生物转化及其代谢产物的潜在抗神经炎症特性

IF 2.6 3区医学

Fitoterapia Pub Date : 2025-08-18 DOI: 10.1016/j.fitote.2025.106828

Wei Liu , Liutong Guan , Wen Xu, Yaoyu Liu, Wenli Wang, Boyi Fan, Guangtong Chen

{"title":"Biotransformation of oleanonic acid from jujube by food fermentation fungus Actinomucor elegans and the potential anti-neuroinflammatory properties of metabolites","authors":"Wei Liu , Liutong Guan , Wen Xu, Yaoyu Liu, Wenli Wang, Boyi Fan, Guangtong Chen","doi":"10.1016/j.fitote.2025.106828","DOIUrl":"10.1016/j.fitote.2025.106828","url":null,"abstract":"<div><div>This study investigated the biotransformation of oleanonic acid <strong>(1),</strong> a major bioactive constituent of jujube, using <em>Actinomucor elegans</em>, a filamentous fungus commonly employed in food fermentation. The process yielded 12 previously undescribed metabolites (compounds <strong>4</strong>, <strong>5</strong>, <strong>7</strong>, <strong>10</strong>, <strong>11</strong>, and <strong>14</strong>–<strong>20</strong>), along with seven known compounds. Structural elucidation of these metabolites was conducted through comprehensive spectroscopic analyses. The anti-neuroinflammatory activities of these metabolites were investigated by assessing their ability to inhibit nitric oxide (NO) production in BV-2 microglial cells stimulated by lipopolysaccharide (LPS). Notably, compounds <strong>4</strong>, <strong>5</strong>, <strong>10</strong>, and <strong>13</strong> exhibited greater inhibitory activity compared to oleanonic acid. Among them, compound <strong>10</strong> demonstrated remarkable efficacy, significantly reducing the transcription levels of inflammation-associated factors, including IL-6, IL-1<em>β</em>, COX-2, and TNF-<em>α</em>. Furthermore, compound <strong>10</strong> enhanced autophagy activation by upregulating the expression of Beclin 1 and LC3 II at the protein level, underscoring its possible inflammation-reducing effects.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106828"},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144863435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0