具有促血管生成活性的溶洞真菌Roussoella sp. LCP1-2次生代谢物

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-08-19 DOI:10.1016/j.fitote.2025.106838

Caixia Wei , Jian Sun , Jingyuan Tian , Ting Shu , Rongxiang Wu , Yi Hao , Kexin Cheng , Cong Wang , Fandong Kong , Liman Zhou

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引用次数: 0

摘要

从Roussoella sp. LCP1-2发酵液中分离得到6个化合物，包括4个新的代谢物：papyracillic acid D (1), 7-epi-4-epi-papyracillic acid B (2), 4-epi-papyracillic acid B (3), 3- hydroxy- 11α，18- di羟基-7-oxo-13-epi- - -pimara-8(9),15-diene(5)，以及2个已知的化合物papyracillic acid B(4)和saccharonol A(6)。通过对核磁共振和质谱数据的综合分析，确定了它们的平面结构。单晶x射线衍射分析明确了1的结构。化合物2、3、5的绝对构型通过实验ECD光谱和计算ECD计算相结合得到。生物学评价表明，化合物3在浓度为40 μM的斑马鱼模型中具有中等促血管生成活性。

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Secondary metabolites from the karst cave fungus Roussoella sp. LCP1-2 with proangiogenic activity

Six compounds were isolated from the fermentation broth of Roussoella sp. LCP1–2, including four new metabolites: papyracillic acid D (1), 7-epi-4-epi-papyracillic acid B (2), 4-epi-papyracillic acid B (3), and 3-hydroxyl-11α,18-Dihydroxy-7-oxo-13-epi-ent-pimara-8(9),15-diene (5), along with two known compounds, papyracillic acid B (4) and saccharonol A (6). Their planar structures were elucidated through comprehensive analysis of NMR and MS spectroscopic data. The structure of 1 was unambiguously confirmed by single-crystal X-ray diffraction analysis. The absolute configurations of compounds 2, 3, and 5 were established through a combination of experimental ECD spectroscopy and computational ECD calculations. Biological evaluation revealed that compound 3 exhibited moderate proangiogenic activity in a zebrafish model at a concentration of 40 μM.

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.