Wan-Bi Feng , Ming-Shang Liu , Tong-Xin Zheng , Zi-Xin Lin , Hai-Wei Xu , Hai-Tao Cai , Yu-Bo Zhang , Jia-Lue Bai , Mao-Xun Yang , Hui Luo , Xiao-San Li
{"title":"中国海草块茎中新的多羟基葫芦烷型三萜及其细胞毒活性","authors":"Wan-Bi Feng , Ming-Shang Liu , Tong-Xin Zheng , Zi-Xin Lin , Hai-Wei Xu , Hai-Tao Cai , Yu-Bo Zhang , Jia-Lue Bai , Mao-Xun Yang , Hui Luo , Xiao-San Li","doi":"10.1016/j.fitote.2025.106840","DOIUrl":null,"url":null,"abstract":"<div><div>Phytochemical investigation of the 95 % ethanol extract of the tubers of <em>Hemsleya chinensis</em> led to the isolation of five previously undescribed cucurbitane-type triterpenoids (<strong>1–5</strong>, named hemslyencins G-K), along with five known analogues (<strong>6–10</strong>). The structures of these compounds were elucidated through comprehensive spectroscopic analysis (UV, IR, HR-ESI-MS, 1D and 2D NMR data). Compounds <strong>1</strong>, <strong>2</strong>, <strong>7</strong>, <strong>9</strong>, and <strong>10</strong> are characterized as 24‑oxygenated cucurbitane-type triterpenoids, were rarely found in the genus <em>Hemsleya</em>. The cytotoxic activities of <strong>1–10</strong> were evaluated against MCF-7, HCT-116, HeLa, and HepG2 cancer cell lines, in which compounds <strong>1</strong> and <strong>8–10</strong> exhibited significant cytotoxic activities with the value of 0.382–13.77 μM. Further mechanism studies displayed that compound <strong>1</strong> dose-dependently induced apoptosis and caused G0/G1 phase cell cycle arrest in MCF-7 cells<em>.</em></div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"186 ","pages":"Article 106840"},"PeriodicalIF":2.6000,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"New polyhydroxy cucurbitane-type triterpenoids from the tubers of Hemsleya chinensis and their cytotoxic activities\",\"authors\":\"Wan-Bi Feng , Ming-Shang Liu , Tong-Xin Zheng , Zi-Xin Lin , Hai-Wei Xu , Hai-Tao Cai , Yu-Bo Zhang , Jia-Lue Bai , Mao-Xun Yang , Hui Luo , Xiao-San Li\",\"doi\":\"10.1016/j.fitote.2025.106840\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Phytochemical investigation of the 95 % ethanol extract of the tubers of <em>Hemsleya chinensis</em> led to the isolation of five previously undescribed cucurbitane-type triterpenoids (<strong>1–5</strong>, named hemslyencins G-K), along with five known analogues (<strong>6–10</strong>). The structures of these compounds were elucidated through comprehensive spectroscopic analysis (UV, IR, HR-ESI-MS, 1D and 2D NMR data). Compounds <strong>1</strong>, <strong>2</strong>, <strong>7</strong>, <strong>9</strong>, and <strong>10</strong> are characterized as 24‑oxygenated cucurbitane-type triterpenoids, were rarely found in the genus <em>Hemsleya</em>. The cytotoxic activities of <strong>1–10</strong> were evaluated against MCF-7, HCT-116, HeLa, and HepG2 cancer cell lines, in which compounds <strong>1</strong> and <strong>8–10</strong> exhibited significant cytotoxic activities with the value of 0.382–13.77 μM. Further mechanism studies displayed that compound <strong>1</strong> dose-dependently induced apoptosis and caused G0/G1 phase cell cycle arrest in MCF-7 cells<em>.</em></div></div>\",\"PeriodicalId\":12147,\"journal\":{\"name\":\"Fitoterapia\",\"volume\":\"186 \",\"pages\":\"Article 106840\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-08-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fitoterapia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0367326X25004666\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fitoterapia","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0367326X25004666","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
New polyhydroxy cucurbitane-type triterpenoids from the tubers of Hemsleya chinensis and their cytotoxic activities
Phytochemical investigation of the 95 % ethanol extract of the tubers of Hemsleya chinensis led to the isolation of five previously undescribed cucurbitane-type triterpenoids (1–5, named hemslyencins G-K), along with five known analogues (6–10). The structures of these compounds were elucidated through comprehensive spectroscopic analysis (UV, IR, HR-ESI-MS, 1D and 2D NMR data). Compounds 1, 2, 7, 9, and 10 are characterized as 24‑oxygenated cucurbitane-type triterpenoids, were rarely found in the genus Hemsleya. The cytotoxic activities of 1–10 were evaluated against MCF-7, HCT-116, HeLa, and HepG2 cancer cell lines, in which compounds 1 and 8–10 exhibited significant cytotoxic activities with the value of 0.382–13.77 μM. Further mechanism studies displayed that compound 1 dose-dependently induced apoptosis and caused G0/G1 phase cell cycle arrest in MCF-7 cells.
期刊介绍:
Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas:
1. Characterization of active ingredients of medicinal plants
2. Development of standardization method for bioactive plant extracts and natural products
3. Identification of bioactivity in plant extracts
4. Identification of targets and mechanism of activity of plant extracts
5. Production and genomic characterization of medicinal plants biomass
6. Chemistry and biochemistry of bioactive natural products of plant origin
7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.