马齿苋骨架生物碱的抗炎机制研究。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-07-26 DOI:10.1016/j.fitote.2025.106760

Xiaoqian Zhang , Yingdai Zhao , Jinpeng Liu , Hongzhe Zhang , Junjie Yao , Jing Liu , Yujuan Niu , Xixiang Ying

{"title":"马齿苋骨架生物碱的抗炎机制研究。","authors":"Xiaoqian Zhang , Yingdai Zhao , Jinpeng Liu , Hongzhe Zhang , Junjie Yao , Jing Liu , Yujuan Niu , Xixiang Ying","doi":"10.1016/j.fitote.2025.106760","DOIUrl":null,"url":null,"abstract":"<div><div>A new skeleton alkaloid was isolated from <em>Portulaca oleracea</em> L., which was identified as ethyl (2<em>Z</em>,4<em>E</em>,6<em>E</em>)-3-amino-7-(4-amino-6-ethyl-3-glycyl-5-methylpyridin-2-yl)-2-methylocta-2,4,6-trienoate, trivial named, olerapyridin and the structure was clarified through IR, UV, 1D and 2D NMR as well as UHPLC-ESI-Q-TOF/MS methods. Moreover, the bioactivities of anti-inflammation of the compound were investigated via testing RAW 264.7 macrophage cell stimulated by lipopolysaccharide. The results showed that olerapyridin at 5 μ<em>M</em> remarkably downregulated the levels of inflammatory mediators, including IL-1<em>β</em>, the proteins expression levels of iNOS and the mRNA expression levels of COX-2, Additionally, at 15 μ<em>M</em>, olerapyridin effectively reduced the levels of NO and TNF-<em>a</em>, as well as the proteins expression levels of COX-2 and mRNA expression levels of iNOS.</div></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":"185 ","pages":"Article 106760"},"PeriodicalIF":2.6000,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The anti-inflammatory mechanism of a new skeleton alkaloid isolated from Portulaca oleracea L.\",\"authors\":\"Xiaoqian Zhang , Yingdai Zhao , Jinpeng Liu , Hongzhe Zhang , Junjie Yao , Jing Liu , Yujuan Niu , Xixiang Ying\",\"doi\":\"10.1016/j.fitote.2025.106760\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>A new skeleton alkaloid was isolated from <em>Portulaca oleracea</em> L., which was identified as ethyl (2<em>Z</em>,4<em>E</em>,6<em>E</em>)-3-amino-7-(4-amino-6-ethyl-3-glycyl-5-methylpyridin-2-yl)-2-methylocta-2,4,6-trienoate, trivial named, olerapyridin and the structure was clarified through IR, UV, 1D and 2D NMR as well as UHPLC-ESI-Q-TOF/MS methods. Moreover, the bioactivities of anti-inflammation of the compound were investigated via testing RAW 264.7 macrophage cell stimulated by lipopolysaccharide. The results showed that olerapyridin at 5 μ<em>M</em> remarkably downregulated the levels of inflammatory mediators, including IL-1<em>β</em>, the proteins expression levels of iNOS and the mRNA expression levels of COX-2, Additionally, at 15 μ<em>M</em>, olerapyridin effectively reduced the levels of NO and TNF-<em>a</em>, as well as the proteins expression levels of COX-2 and mRNA expression levels of iNOS.</div></div>\",\"PeriodicalId\":12147,\"journal\":{\"name\":\"Fitoterapia\",\"volume\":\"185 \",\"pages\":\"Article 106760\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fitoterapia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0367326X25003867\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fitoterapia","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0367326X25003867","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}

引用次数: 0

摘要

从马牙苋（Portulaca oleracea L.）中分离到一种新的骨架生物碱，经鉴定为(2Z,4E,6E)-3-氨基-7-（4-氨基-6-乙基-3-甘酰基-5-甲基吡啶-2-基）-2-甲基吡啶-2,4,6-三烯酸酯，并用IR、UV、1D、2D NMR及UHPLC-ESI-Q-TOF/MS等方法对其结构进行了鉴定。此外，通过脂多糖刺激巨噬细胞RAW 264.7，研究化合物的抗炎活性。结果表明，在5 μM下，大鼠鼠藿苷显著下调炎症介质IL-1β水平、iNOS蛋白表达水平和COX-2 mRNA表达水平；在15 μM下，大鼠藿苷有效降低NO、TNF-a水平、COX-2蛋白表达水平和iNOS mRNA表达水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The anti-inflammatory mechanism of a new skeleton alkaloid isolated from Portulaca oleracea L.

查看原文本刊更多论文

The anti-inflammatory mechanism of a new skeleton alkaloid isolated from Portulaca oleracea L.

A new skeleton alkaloid was isolated from Portulaca oleracea L., which was identified as ethyl (2Z,4E,6E)-3-amino-7-(4-amino-6-ethyl-3-glycyl-5-methylpyridin-2-yl)-2-methylocta-2,4,6-trienoate, trivial named, olerapyridin and the structure was clarified through IR, UV, 1D and 2D NMR as well as UHPLC-ESI-Q-TOF/MS methods. Moreover, the bioactivities of anti-inflammation of the compound were investigated via testing RAW 264.7 macrophage cell stimulated by lipopolysaccharide. The results showed that olerapyridin at 5 μM remarkably downregulated the levels of inflammatory mediators, including IL-1β, the proteins expression levels of iNOS and the mRNA expression levels of COX-2, Additionally, at 15 μM, olerapyridin effectively reduced the levels of NO and TNF-a, as well as the proteins expression levels of COX-2 and mRNA expression levels of iNOS.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.