ELECTROPHORESIS最新文献_第3页

Integration of Forward-Scatter Interferometry-Based Refractive Index Detector Into Capillary Electrophoresis With Laser-Induced Fluorescence. 基于前向散射干涉的折射率检测器集成到激光诱导荧光毛细管电泳中。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-09-14 DOI: 10.1002/elps.70031

Miyuru De Silva, Robert C Dunn

{"title":"Integration of Forward-Scatter Interferometry-Based Refractive Index Detector Into Capillary Electrophoresis With Laser-Induced Fluorescence.","authors":"Miyuru De Silva, Robert C Dunn","doi":"10.1002/elps.70031","DOIUrl":"https://doi.org/10.1002/elps.70031","url":null,"abstract":"A simple and inexpensive method for integrating universal refractive index (RI) detection with existing capillary electrophoresis (CE) platforms utilizing epifluorescence detection is demonstrated. The approach uses the same epi-illumination laser source used for fluorescence detection to create a forward-scattered interference pattern for RI detection. The forward-scattered interferometry (FSI) approach only requires the addition of a bicell photodiode detector and mechanism for aligning it in the fringe pattern to add complementary universal RI detection with existing highly specific epi-fluorescence detection. FSI and fluorescence electropherograms illustrate the utility of measuring both channels simultaneously. Because both signals are generated from the same laser source focused into the same detection zone, the electropherograms are perfectly registered. The sample plug is directly detected with FSI in every separation, providing an intrinsic marker of the electroosmotic flow (EOF) without the need for neutral markers or separate measurements. Moreover, continuous EOF monitoring is also demonstrated using the introduction of thermal markers that are sensed in the FSI signal. Finally, it is shown that the FSI signal responds to both RI and the separation voltage, as has been shown previously for back-scatter interferometry (BSI) detection. This leads to enhanced signal-to-noise at elevated separation voltages, conditions that reduce analysis time and improve peak efficiency.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Sensitive Determination of Bacillus subtilis by Microchip Electrophoresis Combining With A Dual Nucleic Acid Recycling Amplification. 芯片电泳结合双核酸循环扩增高灵敏度测定枯草芽孢杆菌。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-09-10 DOI: 10.1002/elps.70034

Shuang Tang, Qihui Xie, Jianan Lv, Xing Geng, Fan Zhang, Qingjiang Wang

引用次数: 0

Modification of Amino-Functionalized Organic-Silica Hybrid Monoliths via a Catalyst-Free Amino-Yne Click Reaction for Capillary Liquid Chromatography. 用无催化剂的氨基键合反应修饰氨基功能化有机硅杂化单体。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-09-08 DOI: 10.1002/elps.70022

Zedong Huang, Haoran Wang, Wenfeng He, Shulei Qin, Yuxuan Chen, Shanshan Cai, Jun Luo, Miaoduo Deng

{"title":"Modification of Amino-Functionalized Organic-Silica Hybrid Monoliths via a Catalyst-Free Amino-Yne Click Reaction for Capillary Liquid Chromatography.","authors":"Zedong Huang, Haoran Wang, Wenfeng He, Shulei Qin, Yuxuan Chen, Shanshan Cai, Jun Luo, Miaoduo Deng","doi":"10.1002/elps.70022","DOIUrl":"https://doi.org/10.1002/elps.70022","url":null,"abstract":"A novel post-modification strategy was developed for rapid functionalization of monoliths through amino-yne click chemistry. This approach enabled the conjugation of activated alkynes onto amino-functionalized organic-silica hybrid monolith surfaces under mild, catalyst-free conditions. Systematic investigation of critical reaction parameters was conducted to optimize the post-modification process. The morphological and structural characteristics of the prepared monolith were characterized by scanning electron microscopy (SEM) and nitrogen adsorption measurements. The successful grafting of functional groups onto the monolith surface was confirmed by contact angle analysis, Fourier transform infrared (FT-IR) spectroscopy, and x-ray photoelectron spectroscopy (XPS). The functionalized monolith demonstrated chromatographic selectivity for diverse analytes, including phenolic compounds and some weakly polar/nonpolar compounds (benzoin/benzoin methyl ether, styrene/p-chlorostyrene, and 1-naphthylethylamine/naphthalene). Furthermore, it was successfully applied to the quantitative analysis of honokiol in authentic magnolol samples, showcasing its potential for practical analytical applications.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Low-Voltage-Driven Droplet Sorter for High-Stability and Small-Deformation Droplet Sorting. 一种用于高稳定性和小变形液滴分选的低压驱动液滴分选机。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-09-08 DOI: 10.1002/elps.70025

Lin Jiang, Qingqing Liu, Hang Yang, Qinhong Wang, Nan Xiang

{"title":"A Low-Voltage-Driven Droplet Sorter for High-Stability and Small-Deformation Droplet Sorting.","authors":"Lin Jiang, Qingqing Liu, Hang Yang, Qinhong Wang, Nan Xiang","doi":"10.1002/elps.70025","DOIUrl":"https://doi.org/10.1002/elps.70025","url":null,"abstract":"Electric droplet sorting is widely applied in the screening of target molecules, cells, drugs, and microparticles. Previous studies have made several optimizations on the electrode materials, structures, and arrangements. However, voltages of over 1 kV are required to realize droplet sorting, which causes the undesired droplet splitting. In addition, great droplet deformation caused by the high voltage decreases the sorting accuracy and generates negative effects on the targets encapsulated within the droplets. In this study, we developed a low-voltage-driven droplet sorter (LV-DS) for droplet sorting with high stability and small deformation. Our LV-DS consisted of one square active and four triangular ground electrodes which were fabricated using liquid metal with higher stability and higher electrical conductivity than the previous electrode materials of low-melting alloy and salt water. Our LV-DS could realize the high-stability droplet sorting with small deformation at the voltage as low as 80 V. The results showed that our LV-DS presented great prospects in the high-stability droplet sorting with a low voltage. In addition, the advantage of small droplet deformation makes our LV-DS be attractive for the applications, such as droplet-based cell sorting with a high viability and droplet-based material screening with a small deformation.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Hidden Influence: Impacts of Residual Dimethylformamide in NDSB-211 on icIEF Separation for Monoclonal Antibodies. 隐性影响：NDSB-211中残留二甲基甲酰胺对单克隆抗体icIEF分离的影响。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-09-08 DOI: 10.1002/elps.70024

Delaney Doran, Matthew Myers, Katarina Moravcevic, Steven LaBrenz, Pinger Wang, Xiaoying Ji

{"title":"The Hidden Influence: Impacts of Residual Dimethylformamide in NDSB-211 on icIEF Separation for Monoclonal Antibodies.","authors":"Delaney Doran, Matthew Myers, Katarina Moravcevic, Steven LaBrenz, Pinger Wang, Xiaoying Ji","doi":"10.1002/elps.70024","DOIUrl":"https://doi.org/10.1002/elps.70024","url":null,"abstract":"Monoclonal antibodies (mAbs) present analytical challenges due to their inherent heterogeneity and susceptibility to post-translational modifications (PTMs) during production and storage. Monitoring of charge heterogeneity profiles by imaged capillary isoelectric focusing (icIEF) has been aided by the use of non-detergent sulfobetaines (NDSBs), particularly NDSB-211, to enhance protein solubility and stability. When used in a quality control laboratory setting, NDSB-211 has shown performance variability over time due to residual manufacturing impurities that impact the capillary isoelectric focusing separation. Dimethylformamide (DMF) was detected using 1H nuclear magnetic resonance (NMR) in several lots of NDSB-211 that simultaneously produced poor resolution of basic variants of a mAb on icIEF. A parallel spiking study using DMF at 1%-10% (v/v) on 1H NMR and icIEF illustrates the interference in charge heterogeneity separation when a large amount of DMF is present in the NDSB-211 reagent. As DMF is not found on the NDSB-211 certificate of analysis from any vendor, more traditional additives for icIEF are suggested to limit the potential interference for analytical methods.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis. 间接毛细管电泳法研究山奈啶对食源性致病菌的抑菌作用。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-09-08 DOI: 10.1002/elps.70023

Qingqing Wang, Junlan Ye, Wei Wang

{"title":"Study of Bacteriostasis of Kaempferide on Foodborne Pathogenic Bacteria by Indirect Determination of Capillary Electrophoresis.","authors":"Qingqing Wang, Junlan Ye, Wei Wang","doi":"10.1002/elps.70023","DOIUrl":"https://doi.org/10.1002/elps.70023","url":null,"abstract":"Foodborne pathogenic bacteria always threaten human health. Flavonoids are commonly used in antibacterial applications. Studying the antibacterial effect of flavonoids on bacteria is significant. Capillary electrophoresis is a versatile separation means. It has different separation modes, so, it can be applied in the analysis of both bacteria and flavonoids. In this study, an efficient method of indirect separation and detection of three bacteria Vibrio parahaemolyticus, Escherichia coli, and Staphylococcus aureus was developed. The determination of bacteria was realized by separating the aptamers corresponding to the bacteria by capillary sieving electrophoresis. This method avoided the disadvantages of low resolution, channel adsorption and bacterial aggregation, encountered in the direct separation of bacteria. The separation of the flavonoid kaempferide was realized by capillary zone electrophoresis, and kaempferide in Kaempferia galanga was determined. Kaempferide and the extract of Kaempferia galanga were applied in the antibacterial tests separately. Using the developed method, the bacterial growth curves and the curves of the kaempferide concentration were studied respectively. The results showed that both kaempferide and Kaempferia galanga could effectively inhibit the bacteria. The inhibitory effect on the terrestrial bacteria Escherichia coli, and Staphylococcus aureus was better than that on the marine bacterium Vibrio parahaemolyticus.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the Specific Binding Between Aptamers and Cytochrome c With Pressure-Assisted Capillary Electrophoresis Frontal Analysis. 用压力辅助毛细管电泳正面分析表征适体与细胞色素c的特异性结合。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-09-01 DOI: 10.1002/elps.70018

Shuanghao Wang, Chunliang Li, Shuangshuang Wang, Huihui Li, David Da Yong Chen

{"title":"Characterization of the Specific Binding Between Aptamers and Cytochrome c With Pressure-Assisted Capillary Electrophoresis Frontal Analysis.","authors":"Shuanghao Wang, Chunliang Li, Shuangshuang Wang, Huihui Li, David Da Yong Chen","doi":"10.1002/elps.70018","DOIUrl":"https://doi.org/10.1002/elps.70018","url":null,"abstract":"Cytochrome c (cyt c) is a heme protein located in the mitochondrial intermembrane space. Because the release of cyt c is a highly specific event in apoptotic signaling, it can serve as an apoptosis-related marker. To date, three frequently used aptamers for cyt c (Apt40, Apt61, and Apt76) have been selected and applied in the field of sensing. The response of these aptamers is not clear, partly because of their weak affinity and nonspecific binding inherent to the system. In this study, pressure-assisted capillary electrophoresis frontal analysis (PACE-FA) was used to characterize the interactions between the aptamers and cyt c, and an electrophoretic mobility-based correction was introduced to obtain accurate binding constants. A nonlinear curve-fitting approach was used for evaluating specific binding interactions in the presence of nonspecific binding. Apt76 was found to bind specifically to cyt c, exhibiting the highest binding constant (1.53 × 106 M-1), and all three aptamers interacted with cyt c at 1:1 stoichiometry. Fluorescence titrations were performed to verify the effectiveness of the reference-free PACE-FA method. This study demonstrates that specific binding between biomolecules has different characteristics compared to nonspecific binding and that the PACE-FA method can be widely used in the evaluation of biological macromolecular interactions.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proof of Concept for Flow Through Nanoparticle Trapping Using a Dielectrophoretic Metal-Coated Nanofiber Mat. 使用介电金属涂层纳米纤维垫进行纳米颗粒捕获流动的概念证明。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-08-31 DOI: 10.1002/elps.70019

Tonoy K Mondal, Christian Baryla, Hannah Stanley, Stuart J Williams

{"title":"Proof of Concept for Flow Through Nanoparticle Trapping Using a Dielectrophoretic Metal-Coated Nanofiber Mat.","authors":"Tonoy K Mondal, Christian Baryla, Hannah Stanley, Stuart J Williams","doi":"10.1002/elps.70019","DOIUrl":"https://doi.org/10.1002/elps.70019","url":null,"abstract":"While traditional dielectrophoretic methods for nanoparticle enrichment and filtration are versatile and selective, they struggle to handle higher throughput applications. To address this challenge and enhance the practical application of dielectrophoresis, we propose an innovative design for porous sandwiched nanofiber electrodes. The electrode is fabricated through a simple process involving the electrospinning of nanofibers with a diameter of 216 ± 28 nm and mat thickness of around 70 µm, followed by the deposition of a thin chromium/gold layer (approximately 140 nm thick) on both sides. This process ensures no electrical short circuit occurs between the electrodes, and it maintains a sheet resistance of 7.19 Ω/□. The resulting significant electric field gradients are capable of trapping nanoparticles with diameters of 100 nm and 40 nm. The structure's sub-micrometer features and large active surface area allow for trapping of nanoparticles at a flow rate of 3.6 mL/h. To evaluate the effects of applied voltage and volumetric flow rate, we conducted experiments with constant voltage while varying the flow rate and constant flow rate while varying the voltage. Our findings indicate that trapping performance improves with higher AC voltage but decreases at higher flow rates. These insights are crucial for optimizing parameters for large-scale nanoparticle enrichment and filtration. This proof-of-concept study for flow through dielectrophoresis of nanoparticles paves the way for a device suitable for large-scale sample processing and higher throughput/separation efficiency in practical settings.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the Reciprocal Interaction Between Tumor Cells and Macrophages Based on the Biomimic Microfluidic Device. 基于仿生微流控装置的肿瘤细胞与巨噬细胞相互作用研究。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-08-28 DOI: 10.1002/elps.70020

Shuxuan Jin, Qian Wu, Shiqi Chang, Shaojiang Zeng, Jiqiu Yin, Huipeng Ma

{"title":"Study of the Reciprocal Interaction Between Tumor Cells and Macrophages Based on the Biomimic Microfluidic Device.","authors":"Shuxuan Jin, Qian Wu, Shiqi Chang, Shaojiang Zeng, Jiqiu Yin, Huipeng Ma","doi":"10.1002/elps.70020","DOIUrl":"https://doi.org/10.1002/elps.70020","url":null,"abstract":"Tumor-macrophage interactions play a key role in various physiological and pathological processes, such as angiogenesis, immune suppression, and extracellular matrix remodeling. In this study, a biomimetic microfluidic chip was developed to simulate the immune microenvironment of glioma through the co-culture of glioma cells and macrophages in a three-dimensional (3D) matrix. Glioma cells were embedded in collagen I solution after forming spheroids in the microwell array chip and subsequently co-cultured with macrophages in different channels. This chip enabled the real-time monitoring of morphological changes in macrophages, the invasion of glioma cell spheroids, and molecular interactions between different cell types. Two distinct cell types could be extracted and isolated in situ for subsequent molecular biological detection, such as Western blotting or qPCR. The results demonstrated that glioma cell spheroids significantly enhanced invasiveness in the presence of macrophages. Moreover, the phenotype of macrophages altered from M0 to M2 (tumor-supportive) under the influence of tumor cells. The molecular mechanism mediating this reciprocal process was extensively explored. It is believed that this 3D microfluidic tumor model could serve as a useful tool for studying the biological properties of the glioma microenvironment. In addition, a more comprehensive understanding of the mechanisms involved in glioma metastasis could be obtained, especially of how tumor inflammatory cells, including tumor-associated macrophages (TAM), affect invasion process.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Proteinogram in the Free-Living Brazilian Common Opossum (Didelphis aurita). 自由生活巴西负鼠（Didelphis aurita）血清蛋白图谱。

IF 2.5 3区生物学

ELECTROPHORESIS Pub Date : 2025-08-23 DOI: 10.1002/elps.70016

Andrés Mauricio Ortega Orozco, Lucas Drumond Bento, Pollyanna Cordeiro Souto, Fabrícia Modolo Girardi, Verônica Rodrigues Castro, João Vitor Gonçalves de Oliveira, Camilo José Ramirez Lopez, Edvaldo Barros, Artur Kanadani Campos, Leandro Abreu da Fonseca

{"title":"Serum Proteinogram in the Free-Living Brazilian Common Opossum (Didelphis aurita).","authors":"Andrés Mauricio Ortega Orozco, Lucas Drumond Bento, Pollyanna Cordeiro Souto, Fabrícia Modolo Girardi, Verônica Rodrigues Castro, João Vitor Gonçalves de Oliveira, Camilo José Ramirez Lopez, Edvaldo Barros, Artur Kanadani Campos, Leandro Abreu da Fonseca","doi":"10.1002/elps.70016","DOIUrl":"https://doi.org/10.1002/elps.70016","url":null,"abstract":"Didelphis aurita is a synanthropic marsupial widely distributed in southeastern Brazil, known for its resistance to venom and its relevance in biomedical research. This study aimed to characterize the serum proteinogram of free-living D. aurita individuals. Blood samples from 27 animals, classified as \"healthy\" or \"diseased,\" were analyzed. Eighteen protein bands were identified, with molecular weights ranging from 24 to 242 kDa. Among these, variations in specific bands were associated with health status (band J), sex (bands D, M, N, and P), and age (bands N and P). Mass spectrometry (liquid chromatography-tandem mass spectrometry [LC-MS/MS]) identified seven proteins, including DM64, ceruloplasmin, von Willebrand factor A (VWFA) domain protein, alpha-2-macroglobulin, fibronectin, and actin depolymerizing factor. These results highlight the influence of biological factors on serum protein profiles and reinforce the potential of D. aurita as a model for immunological and proteomic studies.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144947010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0