ELECTROPHORESIS最新文献_第3页

Enhanced Green Fluorescent Protein Streaming Dielectrophoresis in Insulator-Based Microfluidic Devices. 绝缘体基微流体设备中的增强型绿色荧光蛋白流介电泳。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-11 DOI: 10.1002/elps.202400123

Jerry Sheu, Sean Seyler, A K M Fazlul Karim Rasel, Mark A Hayes

{"title":"Enhanced Green Fluorescent Protein Streaming Dielectrophoresis in Insulator-Based Microfluidic Devices.","authors":"Jerry Sheu, Sean Seyler, A K M Fazlul Karim Rasel, Mark A Hayes","doi":"10.1002/elps.202400123","DOIUrl":"https://doi.org/10.1002/elps.202400123","url":null,"abstract":"There is tantalizing evidence that proteins can be accurately and selectively manipulated by higher order electric field effects within microfluidic devices. The accurate and precise manipulation of proteins in these platforms promises to disrupt and revolutionize many fields, most notably analytical biochemistry. Several lines of experimental evidence suggest much higher forces are generated compared to those calculated from traditional theories and those higher forces arise from subtle structural features of the proteins providing selectivity. New theories reflect some of the experimental evidence in the magnitude of the force predicted and inclusion of subtle structural features absent in traditional continuum theory. Unfortunately, the experimental evidence is largely exploratory in nature and lacks one or more important elements that prevents a clear interpretation and comparison to not only the other existing data, but also quantitative comparison to the evolving theoretical descriptions. Here, a clear and interpretable experimental system is presented that quantitatively determines the dielectrophoretic susceptibility of unlabeled, unaggregated native-structure protein molecules that are exposed to modest electric fields (105-106 V/m) for short periods of time (∼5 ms) without significant increases in local concentration. The platform uses sub-nanogram quantities of protein, the probed volume upon determination is a few picoliters, and the total analysis time is 10 s.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty Acid Analysis by Capillary Electrophoresis and Contactless Conductivity Detection for Future Life Detection Missions. 利用毛细管电泳和非接触式电导检测进行脂肪酸分析，用于未来的生命探测任务。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-11 DOI: 10.1002/elps.202400162

Miranda G M Kok, Maria F Mora

{"title":"Fatty Acid Analysis by Capillary Electrophoresis and Contactless Conductivity Detection for Future Life Detection Missions.","authors":"Miranda G M Kok, Maria F Mora","doi":"10.1002/elps.202400162","DOIUrl":"https://doi.org/10.1002/elps.202400162","url":null,"abstract":"Future life-detection missions will likely search for biosignatures within a wide range of organic compounds, including fatty acids. In order to determine such biosignatures, it is necessary to identify and quantify individual fatty acids present within a sample. In this study, we present a method using capillary electrophoresis coupled to contactless conductivity detection (CE-C4D) for the separation and detection of both saturated and unsaturated fatty acids after derivatization with N,N-diethylethylenediamine, triethylamine, and 2-chloro-1-methylpyridinium iodide at 40°C for 10 min. Operating conditions (background electrolyte, separation voltage, and temperature) were optimized to provide maximum separation of fatty acids, thereby allowing their identification and quantification. Using a background electrolyte of 2 M acetic acid in 45% acetonitrile, an optimal separation was obtained with a separation voltage of 10 kV and a capillary temperature of 15°C. The optimized CE-C4D method was used to analyze samples of the cyanobacterium Spirulina. Multiple fatty acids were detected in the samples, showing the potential of this method for detection of fatty acid biosignatures during future spaceflight missions.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of Viscous Jeffrey Fluid Flow Through Darcian Medium With Hall Current and Quadratic Buoyancy. 带有霍尔电流和二次浮力的粘性杰弗里流体流经达尔西亚介质的动力学。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-11 DOI: 10.1002/elps.202400078

Mojeed T Akolade, Samson A Agunbiade, Timothy L Oyekunle, Peter B Malgwi

{"title":"Dynamics of Viscous Jeffrey Fluid Flow Through Darcian Medium With Hall Current and Quadratic Buoyancy.","authors":"Mojeed T Akolade, Samson A Agunbiade, Timothy L Oyekunle, Peter B Malgwi","doi":"10.1002/elps.202400078","DOIUrl":"https://doi.org/10.1002/elps.202400078","url":null,"abstract":"This contribution builds on existing studies by investigating the dynamics of Hall current in Jeffery fluid under radiative heat, convective boundary conditions, Joule heating, and Darcy dissipation. Hall current, an important phenomenon in engineering applications involving strong magnetic fields, highlights the impact of electromagnetic force in examining blood flow rate, determining charge drift velocity, density, and movement, and is used in power generators and high-voltage transformers. This analysis incorporates dissipative and thermal radiative heat and employs the effects of Hall current and Joule heating, resulting from porous medium resistance, to derive the partial differential equations governing the dynamic systems. These equations are then reduced to ordinary differential equations (ODEs) through similarity variables. The Galerkin weighted residual method (GWRM) is employed to examine the dynamics of Hall current and quadratic thermal buoyancy, shedding light on the thermal properties and hydrodynamics of Jeffrey fluid convection within a porous medium. The analysis reveals that in the presence of an applied magnetic field, the contribution of Hall current to flow and heat dynamics induces a magnetic force that enhances fluid motion and negatively impacts heat energy patterns. The imposition of dissipative heat physically increases the fluid temperature, owing to an increase in buoyancy current. The occurrence of thermal radiation, Hall current, viscous dissipation, and Joule heating can efficiently optimize the rate of heat transfer and shear stress. Moreover, the tabular results indicate that Jeffrey fluid, exhibiting higher relaxation time, will experience a lower friction coefficient and heat transfer rate.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Micro-Flow Liquid Chromatography-Mass Spectrometry Method for the Quantification of Oxylipins in Volume-Limited Human Plasma. 微流液相色谱-质谱法定量测定容积有限的人体血浆中的氧脂类物质

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-11 DOI: 10.1002/elps.202400151

Bingshu He, Rawi Ramautar, Marian Beekman, P Eline Slagboom, Amy Harms, Thomas Hankemeier

{"title":"A Micro-Flow Liquid Chromatography-Mass Spectrometry Method for the Quantification of Oxylipins in Volume-Limited Human Plasma.","authors":"Bingshu He, Rawi Ramautar, Marian Beekman, P Eline Slagboom, Amy Harms, Thomas Hankemeier","doi":"10.1002/elps.202400151","DOIUrl":"https://doi.org/10.1002/elps.202400151","url":null,"abstract":"Oxylipins are well-known lipid mediators in various inflammatory conditions. Their endogenous concentrations range from low picomolar to nanomolar, and there are growing demands to determine their concentrations in low-volume matrices for pathological studies, including blood, cerebrospinal fluids from animal disease models, infants, and microsampling devices. Most of the published quantification methods for comprehensive profiling of oxylipins still require more than 50 µL plasma as a starting volume to detect these low levels. The aim of our study is to develop a sensitive and reliable method for the quantification of oxylipins in volume-limited human plasma samples. We established and validated a micro-liquid chromatography (LC)-mass spectrometry (MS)/MS method that requires only 5 µL of human plasma for the determination of 66 oxylipins. The optimized micro-LC-MS/MS method utilized a flow rate of 4 µL/min with a 0.3-mm inner diameter column. With an injection volume of 3 µL, our method provides limits of detection in the range from 0.1 to 91.9 pM, and limits of quantification range from 0.3 to 306.2 pM. The sensitivity enhancement compared to conventional flow ranged from 1.4 to 180.7 times for 51 compounds depending on their physical-chemical properties. After validation, the method was applied to analyze 40 plasma samples from a healthy aging study to demonstrate robustness and sensitivity.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of Silver Nanoparticles in a Water-in-Oil Microemulsion Stabilized by Ecosurf EH3 and Determination of Their Electrophoretic Mobility 在经 Ecosurf EH3 稳定的油包水微乳液中制备银纳米粒子并测定其电泳迁移率

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-07 DOI: 10.1002/elps.202400200

Pavel S. Popovetskiy, Sofia A. Petrochenko

{"title":"Preparation of Silver Nanoparticles in a Water-in-Oil Microemulsion Stabilized by Ecosurf EH3 and Determination of Their Electrophoretic Mobility","authors":"Pavel S. Popovetskiy, Sofia A. Petrochenko","doi":"10.1002/elps.202400200","DOIUrl":"10.1002/elps.202400200","url":null,"abstract":"This work describes a study on the electrophoresis of silver nanoparticles in reverse microemulsions with varying water content. The microemulsion was stabilized using a nonionic ethoxylated surfactant, 2-ethylhexanol triethoxylate (Ecosurf EH3). This study represents the second example of electrophoresis research conducted in media with a low dielectric constant for etoxylated surfactants. The study also determined the boundaries of thermodynamic stability and the conditions required to obtain nanoparticles with a high yield. The hydrodynamic diameter and electrophoretic mobility of nanoparticles were measured using dynamic light scattering and laser Doppler electrophoresis. The study determined the boundary conditions for applying these methods to laser-absorbing samples. The electrophoretic mobility of nanoparticles was found to be dependent on the fraction of water in the range of 2–5% vol. (equivalent to a metal content of 10–25 mM), as determined by electrophoresis in a free medium. The increase in volume fraction of water leads to agglomeration of micelles, which causes a decrease in the electrokinetic potential of nanoparticles, likely due to the blurring of the diffuse part of the electrical double layer.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 21-22","pages":"1955-1962"},"PeriodicalIF":3.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental Validation of a DIP-InDel/DIP-STR System for the Detection of Unbalanced DNA Mixtures. 用于检测不平衡 DNA 混合物的 DIP-InDel/DIP-STR 系统的开发验证。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-07 DOI: 10.1002/elps.202400119

Mingming Zhang, Yuxin Zhang, Hailing Yang, Huan Yu, Jiajia Fan, Jiaqi Wang, Zidong Liu, Jinding Liu, Jianbo Ren, Gengqian Zhang

{"title":"Developmental Validation of a DIP-InDel/DIP-STR System for the Detection of Unbalanced DNA Mixtures.","authors":"Mingming Zhang, Yuxin Zhang, Hailing Yang, Huan Yu, Jiajia Fan, Jiaqi Wang, Zidong Liu, Jinding Liu, Jianbo Ren, Gengqian Zhang","doi":"10.1002/elps.202400119","DOIUrl":"https://doi.org/10.1002/elps.202400119","url":null,"abstract":"Extracting \"masked\" minor donor information from an unbalanced DNA mixture stain is important for the identification of the person of interest. In recent years, a series of compound markers genotyped based on allele-specific amplifications have been proposed to detect minor contributors of mixed stains. In this study, we selected out 18 DIP-InDel markers from previous literatures and established a multiplex system encompassing 18 DIP-InDel markers and 6 DIP-STR markers in two separate reactions. The allele frequencies were estimated based on 200 samples, and 23 of the 24 DIP-linked length polymorphic markers had a relatively high probability of informative markers (I value >0.267), which indicated their potential usefulness in the Chinese Han population. Moreover, the multiplex was highly sensitive (requiring >0.025 ng) and specific to human DNA. This system is capable of detecting the minor contributor comprising 1% in unbalanced mixtures of two individuals. The capacity of profiling cell-free DNA was also analyzed in few cases. At least one paternal allele could be detected in maternal plasma samples with gestation periods ranging from 27 to 40 weeks. To conclude, the DIP-linked length polymorphic markers may serve as a convenient method for detecting the presence of a minor donor in unbalanced mixtures and show promise for noninvasive prenatal diagnosis.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Review on Capillary Electrophoresis-Mass Spectrometry in Advancing Biomolecular Research. 全面评述毛细管电泳-质谱法在推进生物分子研究中的应用。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-07 DOI: 10.1002/elps.202400122

Laura Pont, María Vergara-Barberán, Enrique Javier Carrasco-Correa

引用次数: 0

mRNA for Body Fluid and Individual Identification. 用于体液和个体识别的 mRNA。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-05 DOI: 10.1002/elps.202400077

Zidong Liu, Jiaqi Wang, Zeqin Li, Gengqian Zhang

引用次数: 0

Recent Progress and Applications of Advanced Nanomaterials in Solid-Phase Extraction. 先进纳米材料在固相萃取中的最新进展和应用。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-05 DOI: 10.1002/elps.202400152

Pin Chen, Qiuyue Zhang, Hang Yin, Siyuan Di, Huan Liu, Hailan Qin, Ming Liu, Yunkang Liu, Zihan Li, Shukui Zhu

{"title":"Recent Progress and Applications of Advanced Nanomaterials in Solid-Phase Extraction.","authors":"Pin Chen, Qiuyue Zhang, Hang Yin, Siyuan Di, Huan Liu, Hailan Qin, Ming Liu, Yunkang Liu, Zihan Li, Shukui Zhu","doi":"10.1002/elps.202400152","DOIUrl":"https://doi.org/10.1002/elps.202400152","url":null,"abstract":"Sample preparation maintains a key bottleneck in the whole analytical procedure. Solid-phase sorbents (SPSs) have garnered increasing attention in sample preparation research due to their crucial roles in achieving high clean-up and enrichment efficiency in the analysis of trace targets present in complex matrices. Novel nanoscale materials with improved characteristics have garnered considerable interest across different scientific disciplines due to the limited capabilities of traditional bulk-scale materials. The purpose of this review is to offer a thorough summary of the latest developments and uses of SPSs in preparing samples for chromatographic analysis, focusing on the years 2020-2024. The techniques for preparing SPSs are examined, such as metal-organic frameworks (MOFs), covalent organic frameworks (COFs), carbon nanoparticles (CNPs), molecularly imprinted polymers (MIPs), and metallic nanomaterials (MNs). Examining the pros and cons of different extraction methods, including solid-phase extraction (SPE), magnetic SPE (MSPE), flow-based SPE (FBA-SPE), solid-phase microextraction (SPME), stir-bar sorptive extraction (SBSE), and dispersive SPE (DSPE), is the main focus. Furthermore, this article presents the utilization of SPE technology for isolating common contaminants in various environmental, biological, and food specimens. We highlight the persistent challenges in SPSs and anticipate future advancements and applications of novel SPSs.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer-Modified Magnetic Beads and Permalloy Micromagnets 使用 Aptamer 改性磁珠和坡莫合金微磁体从混合细胞样本中分离肺癌细胞

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-11-05 DOI: 10.1002/elps.202400147

Shu-Hui Lin, Yun-Jung Tsai, Tzu-Cheng Su, Shih-Lun Lai, Chun-Ping Jen

{"title":"Separation of Lung Cancer Cells From Mixed Cell Samples Using Aptamer-Modified Magnetic Beads and Permalloy Micromagnets","authors":"Shu-Hui Lin, Yun-Jung Tsai, Tzu-Cheng Su, Shih-Lun Lai, Chun-Ping Jen","doi":"10.1002/elps.202400147","DOIUrl":"10.1002/elps.202400147","url":null,"abstract":"<div>\u0000 \u0000 This study involved the design and fabrication of a microfluidic chip integrated with permalloy micromagnets. The device was used with aptamer-modified magnetic beads (MBs) of various sizes to successfully separate lung cancer cells from a mixture of other cells. The overall separation efficiency was evaluated based on the ratios of cells in the different outlets and inlets of the chip. The results showed efficiencies ranging from 43.4% to 50.2% for MB sizes between 1.36 and 4.50 µm. Interestingly, efficiency slightly decreased as the size of the MBs increased, contrary to predictions. Further examination revealed that larger MBs exerted gravitational force on the cell-bound MBs at low flow rates, causing the targets to settle before reaching the main microchannel region. This was attributed to fluidic resistance caused by a size mismatch between the inlet tube and the microfluidic conduit. An increase in cell accumulation at the inlet was observed with larger MB sizes due to gravity. Therefore, the definition of effective separation efficiency was revised to exclude the effect of cell accumulation at the inlet. Effective separation efficiencies were found to be 71.6%, 76.4%, and 79.4% for MB sizes of 1.36, 3.00, and 4.50 µm, respectively. The study concluded that larger MBs interacted more with the magnetic force, resulting in better separation. However, cells with smaller MBs were more likely to evade the magnetic force. The investigation provides valuable insights into isolating lung cancer cells using this method, with the potential for clinical application in cancer diagnosis and treatment.\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 21-22","pages":"2054-2064"},"PeriodicalIF":3.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0