ELECTROPHORESIS最新文献_第10页

An immuno-northern technique to measure the size of dsRNA byproducts in in vitro transcribed RNA 一种用于测量体外转录 RNA 中 dsRNA 副产物大小的免疫北方技术。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-24 DOI: 10.1002/elps.202400036

Nathaniel E. Clark, Matthew R. Schraut, Roger A. Winters, Kelley Kearns, Thomas C. Scanlon

{"title":"An immuno-northern technique to measure the size of dsRNA byproducts in in vitro transcribed RNA","authors":"Nathaniel E. Clark, Matthew R. Schraut, Roger A. Winters, Kelley Kearns, Thomas C. Scanlon","doi":"10.1002/elps.202400036","DOIUrl":"10.1002/elps.202400036","url":null,"abstract":"Double-stranded RNA is an immunogenic byproduct present in RNA synthesized with in vitro transcription. dsRNA byproducts engage virus-sensing innate immunity receptors and cause inflammation. Removing dsRNA from in vitro transcribed messenger RNA (mRNA) reduces immunogenicity and improves protein translation. Levels of dsRNA are typically 0.1%–0.5% of total transcribed RNA. Because they form such a minor fraction of the total RNA in transcription reactions, it is difficult to confidently identify discrete bands on agarose gels that correspond to the dsRNA byproducts. Thus, the sizes of dsRNA byproducts are largely unknown. Total levels of dsRNA are typically assayed with dsRNA-specific antibodies in ELISA and immuno dot-blot assays. Here we report a dsRNA-specific immuno-northern blot technique that provides a clear picture of the dsRNA size distributions in transcribed RNA. This technique could complement existing dsRNA analytical methods in studies of dsRNA byproduct synthesis, dsRNA removal, and characterization of therapeutic RNA drug substances.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141087122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Succession changes of microbial community for inferring the time since deposition of saliva 用于推断唾液沉积时间的微生物群落继承变化。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-22 DOI: 10.1002/elps.202300267

Xiaoye Jin, Shunyi Tian, Hongling Zhang, Zheng Ren, Qiyan Wang, Yubo Liu, Hao Zheng, Meiqing Yang, Jiang Huang

{"title":"Succession changes of microbial community for inferring the time since deposition of saliva","authors":"Xiaoye Jin, Shunyi Tian, Hongling Zhang, Zheng Ren, Qiyan Wang, Yubo Liu, Hao Zheng, Meiqing Yang, Jiang Huang","doi":"10.1002/elps.202300267","DOIUrl":"10.1002/elps.202300267","url":null,"abstract":"Saliva is a common biological examination material at crime scenes and has high application value in forensic case investigations. It can reflect the suspect's time of crime at the scene and provide evidence of the suspect's criminal facts. Even though many researchers have proposed their experimental protocols for estimating the time since deposition (TsD) of saliva, there is still a relative lack of research on the use of microorganisms to estimate TsD. In the current study, the succession change of microbial community in saliva with different TsD values was explored to discern the microbial markers related to TsD of saliva. We gathered saliva samples from six unrelated healthy Han individuals living in Guizhou, China and exposed these samples to indoor conditions at six time points (0, 1, 3, 7, 15, and 28 days). Temporal changes of microbial compositions in these samples were investigated by 16S rRNA sequencing (V3–V4 regions). By assessing temporal variation patterns of microbial abundance at the genus level, four bacteria (Brucella, Prevotella, Pseudomonas, and Fusobacterium) were observed to show good time dependence in these samples. In addition, the hierarchical clustering and principal co-ordinates analysis results revealed that these saliva samples could be classified into t-short (≤7 days) and t-long (>7 days) groups. In the end, the random forest model was developed to predict the TsD of these samples. For the model, the root mean square error, R2, and mean absolute error between predicted and actual TsD values were 1.5213, 0.9851, and 1.1969, respectively. To sum up, we identified TsD-related microbial markers in saliva samples, which could be viewed as valuable markers for inferring the TsD of saliva.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contents: Electrophoresis 9–10'24 内容：电泳 9-10'24

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-22 DOI: 10.1002/elps.202470053

引用次数: 0

Editorial Board: Electrophoresis 9–10'24 编辑委员会：电泳 9-10'24

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-22 DOI: 10.1002/elps.202470052

引用次数: 0

Evaluation of tauroursodeoxycholic acid in liver cells’ cultures by MEKC: Initial hints to comprehend its role in diabetes mellitus of obese individuals 用 MEKC 评估肝细胞培养物中的牛磺脱氧胆酸：初步了解其在肥胖者糖尿病中的作用。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-22 DOI: 10.1002/elps.202300223

Bruna Eduarda Santos Simões, Mariana Roberta Rodrigues Muniz, Thiago dosReis Araujo, Everardo Magalhães Carneiro, Ana Valéria Colnaghi Simionato

{"title":"Evaluation of tauroursodeoxycholic acid in liver cells’ cultures by MEKC: Initial hints to comprehend its role in diabetes mellitus of obese individuals","authors":"Bruna Eduarda Santos Simões, Mariana Roberta Rodrigues Muniz, Thiago dosReis Araujo, Everardo Magalhães Carneiro, Ana Valéria Colnaghi Simionato","doi":"10.1002/elps.202300223","DOIUrl":"10.1002/elps.202300223","url":null,"abstract":"Genetic factors, diet, lifestyle, and other factors lead to various complications in the body, such as obesity and other chronic diseases. The inflammatory state caused by excessive accumulation of body fat affects the pathways related to the control of glycemic homeostasis, leading to a high demand for insulin, to subsequent failure of stressed β cells, and development of type 2 diabetes mellitus (T2DM). The study of new endocrine signalers, such as bile acids (BAs), becomes necessary as it allows the development of alternatives for T2DM treatment. In this work, a methodology was developed to quantify tauroursodeoxycholic BA (TUDCA) in liver cells of the HepG2 strain treated in hyperlipidic medium. This BA helps to improve insulin clearance by increasing the expression of the insulin-degrading enzyme, restoring sensitivity to this hormone, and making it viable for treating T2DM. Herein, a targeted metabolomic method for TUDCA determination in extracellular medium of hepatocyte matrices by micellar electrokinetic chromatography-UV was optimized, validated, and applied. The optimized background electrolyte was composed of 40 mmol/L sodium cholate and 30 mmol/L sodium tetraborate at pH 9.0. The following figures of merit were evaluated: linearity, limit of quantification, limit of detection, accuracy, and precision. Data obtained with the validated electrophoretic method showed a self-stimulation of TUDCA production in media supplemented only with BA. On the other hand, TUDCA concentration was reduced in the hyperlipidic medium. This suggests that, in these media, the effect of TUDCA is reduced, such as self-stimulated production and consequent regulation of glycemic homeostasis. Therefore, the results reinforce the need for investigating TUDCA as a potential T2DM biomarker as well as its use to treat several comorbidities, such as obesity and diabetes mellitus.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Front Cover: Pilot validation of on-field STR typing and human identity testing by MinION nanopore sequencing 封面：利用 MinION 纳米孔测序技术进行现场 STR 分型和人类身份测试的试点验证

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-22 DOI: 10.1002/elps.202470051

Yuan Luo, Jiarong Zhang, Ming Ni, Zhusong Mei, Qiao Ye, Bingqian Guo, Longmei Fang, Dongyun Feng, Lu Wang, Jiangwei Yan, Guangyun Wang

引用次数: 0

Quantitative analysis of therapeutic peptides by CZE using multiple sample injection in hydrodynamically closed separation system. 在流体动力封闭分离系统中使用多样品进样，通过 CZE 对治疗肽进行定量分析。

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-13 DOI: 10.1002/elps.202400039

Ondrej Stefanik, Peter Mikus, Juraj Piestansky

{"title":"Quantitative analysis of therapeutic peptides by CZE using multiple sample injection in hydrodynamically closed separation system.","authors":"Ondrej Stefanik, Peter Mikus, Juraj Piestansky","doi":"10.1002/elps.202400039","DOIUrl":"https://doi.org/10.1002/elps.202400039","url":null,"abstract":"Therapeutic peptides have emerged as an innovative and promising class of therapeutic compounds in modern medicine. Synthetic peptide analogs triptorelin and lanreotide are known for their pronounced clinical versatility and potency. In this study, we present the development and validation of novel methods based on capillary zone electrophoresis performed in hydrodynamically closed system (HCS) and paired with ultraviolet detection and repeated injection sample introduction. To the best of our knowledge, we developed the first capillary electrophoresis-based method for the determination of lanreotide, and concurrently, the first HCS method for the determination of triptorelin. Maximal separation efficiency and signal intensity were achieved using background electrolytes composed of 50 mM formic acid with the addition of 0.05% (v/v) methyl-hydroxyethyl cellulose. The proposed methods exhibit favorable performance characteristics, namely, calibration curve (r2 exceeding 0.99), low limits of detection (0.25 µg/mL in a water matrix and 0.5 µg/mL in synthetic urine), acceptable precision (relative standard deviation ranging from 2.2% to 9.6% for intraday repeatability and between 5.2% and 14.9% for interday reproducibility), and accuracy (relative errors falling within the 91.1%-107.8% range). The method for triptorelin determination was then used for its quantification in a commercially available drug dosage form (powder for injection) and in spiked synthetic urine samples. The developed methods were also evaluated according to the novel blue applicability grade index, revealing their superior applicability. The results collectively point out the potential of the proposed methods for both quality control and clinical investigations.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophoresis on Enantioseparations 2024 对映体分离电泳 2024。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-13 DOI: 10.1002/elps.202470064

Bezhan Chankvetadze

引用次数: 0

Dielectrophoresis: Measurement technologies and auxiliary sensing applications Dielectrophoresis：测量技术和辅助传感应用。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-13 DOI: 10.1002/elps.202300299

Sheng Hu, Junyou Ji, Xiaoming Chen, Ruijie Tong

{"title":"Dielectrophoresis: Measurement technologies and auxiliary sensing applications","authors":"Sheng Hu, Junyou Ji, Xiaoming Chen, Ruijie Tong","doi":"10.1002/elps.202300299","DOIUrl":"10.1002/elps.202300299","url":null,"abstract":"Dielectrophoresis (DEP), which arises from the interaction between dielectric particles and an aqueous solution in a nonuniform electric field, contributes to the manipulation of nano and microparticles in many fields, including colloid physics, analytical chemistry, molecular biology, clinical medicine, and pharmaceutics. The measurement of the DEP force could provide a more complete solution for verifying current classical DEP theories. This review reports various imaging, fluidic, optical, and mechanical approaches for measuring the DEP forces at different amplitudes and frequencies. The integration of DEP technology into sensors enables fast response, high sensitivity, precise discrimination, and label-free detection of proteins, bacteria, colloidal particles, and cells. Therefore, this review provides an in-depth overview of DEP-based fabrication and measurements. Depending on the measurement requirements, DEP manipulation can be classified into assistance and integration approaches to improve sensor performance. To this end, an overview is dedicated to developing the concept of trapping-on-sensing, improving its structure and performance, and realizing fully DEP-assisted lab-on-a-chip systems.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification of the electrokinetic motion of microalgae through light illumination for viability assessment. 通过光照改变微藻的电动运动以评估其活力。

IF 2.9 3区生物学

ELECTROPHORESIS Pub Date : 2024-05-13 DOI: 10.1002/elps.202400040

Deyu Li, Rui Jiang, Jun Li, Zhen Liu, Yasmeen Zamir Ahmed, Qiankun Zhao, Yongxin Song, Mengqi Li

{"title":"Modification of the electrokinetic motion of microalgae through light illumination for viability assessment.","authors":"Deyu Li, Rui Jiang, Jun Li, Zhen Liu, Yasmeen Zamir Ahmed, Qiankun Zhao, Yongxin Song, Mengqi Li","doi":"10.1002/elps.202400040","DOIUrl":"https://doi.org/10.1002/elps.202400040","url":null,"abstract":"The viability detection of microalgae with the electrokinetic (EK) technique shows vast applications in the biology and maritime industry. However, due to the slight variations in the EK properties between alive and dead microalgae cells, the accuracy and practicability of this technique is limited. In this paper, the light illumination pretreatment was conducted to modify the EK velocity of microalgae for enhancing the EK difference. The effects of the illumination time and light color on the EK velocities of Chlorella vulgaris and Isochrysis galbana were systematically measured, and the EK differences between alive and dead cells were calculated and compared. The results indicate that under light illumination, the photosynthesis of the alive cells leads to the amplification of the zeta potential, leading toward increase in the EK difference along with the illumination time. By using light with different color spectra to treat the microalgae, it was found that the EK difference changes with the light color according to the following order: white light > red light > blue light > green light. The difference in EK potential with exposure to white light treatment surpasses over 10-fold in comparison to those without such treatment. The light pretreatment technique, as illustrated in this study, offers an advantageous strategy to enhance the EK difference between living and dead cells, proving beneficial in the field of microalgae biotechnology.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0