ELECTROPHORESIS最新文献_第10页

Possibility of using the imaged capillary isoelectric method as a multi-attribute method for bispecific antibodies 将成像毛细管等电法用作双特异性抗体多属性方法的可能性。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-08 DOI: 10.1002/elps.202400066

Gang Wu, Jialiang Du, Gangling Xu, Meng Li, Chuanfei Yu

引用次数: 0

Enantiomeric separation of nefopam and cathinone derivatives using a supramolecular deep eutectic solvent as a chiral selector in capillary electrophoresis 在毛细管电泳中使用超分子深共晶溶剂作为手性选择器分离奈福泮和卡西酮衍生物的对映体。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-04 DOI: 10.1002/elps.202400092

Katerina A. Ioannou, Maria N. Georgiou, Georgia D. Ioannou, Atalanti Christou, Ioannis J. Stavrou, Martin G. Schmid, Constantina P. Kapnissi-Christodoulou

{"title":"Enantiomeric separation of nefopam and cathinone derivatives using a supramolecular deep eutectic solvent as a chiral selector in capillary electrophoresis","authors":"Katerina A. Ioannou, Maria N. Georgiou, Georgia D. Ioannou, Atalanti Christou, Ioannis J. Stavrou, Martin G. Schmid, Constantina P. Kapnissi-Christodoulou","doi":"10.1002/elps.202400092","DOIUrl":"10.1002/elps.202400092","url":null,"abstract":"The present study investigates the utilization of a supramolecular deep eutectic solvent (SUPRADES), consisting of sulfated-β-cyclodextrin (S-β-CD) and citric acid (CA), as a chiral selector (CS) in capillary electrophoresis for the enantiomeric separation of nefopam (NEF) and five cathinone derivatives (3-methylmethcathinone [3-MMC], 4-methylmethcathinone [4-MMC], 3,4-dimethylmethcathinone [3,4-DMMC], 4-methylethcathinone [4-MEC], and 3,4-methylendioxycathinone [MDMC]). A significant improvement in enantiomeric separation of the target analytes was observed upon the addition of S-β-CD-CA to the background electrolyte (BGE), leading to a baseline separation of all analytes. In particular, the optimum percentage of S-β-CD-CA, added to the BGE, was determined to be 0.075% v/v for NEF (Rs = 1.5) and 0.050% v/v for three out of five cathinone derivatives (Rs = 1.5, 1.6, and 2.4 for 3-MMC, 4-MEC, and 3,4-DMMC, respectively). In the case of 4-MMC and MDMC, a higher percentage of the CS, equal to 0.075% and 0.10% v/v, respectively, was required to achieve baseline separation (Rs = 1.5, 1.9 for MDMC and 4-MMC, respectively). The outcomes of the present study highlight the potential effectiveness of using SUPRADES as a CS in electrophoretic enantioseparations.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 19-20","pages":"1721-1726"},"PeriodicalIF":3.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing water hyacinth plants from two South African rivers for the detection of seven pharmaceuticals and their metabolites. 分析南非两条河流中的水葫芦植物，以检测七种药物及其代谢物。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-04 DOI: 10.1002/elps.202400101

Markus Himmelsbach, Franz Mlynek, Wolfgang Buchberger, Lawrence Madikizela, Christian W Klampfl

引用次数: 0

Local nano-electrode fabrication utilizing nanofluidic and nano-electrochemical control. 利用纳米流体和纳米电化学控制制造局部纳米电极。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-07-04 DOI: 10.1002/elps.202300002

Kyojiro Morikawa, Tomoaki Takeuchi, Takehiko Kitamori

{"title":"Local nano-electrode fabrication utilizing nanofluidic and nano-electrochemical control.","authors":"Kyojiro Morikawa, Tomoaki Takeuchi, Takehiko Kitamori","doi":"10.1002/elps.202300002","DOIUrl":"https://doi.org/10.1002/elps.202300002","url":null,"abstract":"Miniaturized systems have attracted much attention with the recent advances in microfluidics and nanofluidics. From the capillary electrophoresis, the development of glass-based microfluidic and nanofluidic technologies has supported advances in microfluidics and nanofluidics. Most microfluidic systems, especially nanofluidic systems, are still simple, such as systems constructed with simple straight nanochannels and bulk-scale electrodes. One of the bottlenecks to the development of more complicated and sophisticated systems is to develop the locally integrated nano-electrodes. However, there are still issues with integrating nano-electrodes into nanofluidic devices because it is difficult to fit the nano-electrode size into a nanofluidic channel at the nanometer level. In this study, we propose a new method for the fabrication of local nano-electrodes in nanofluidic devices with nanofluidic and nano-electrochemistry-based experiments. An electroplating solution was introduced to a nanochannel with control of the flow and the electroplating reaction, by which nano-electrodes were successfully fabricated. In addition, a nanofluidic device was available for nanofluidic experiments with the application of 200 kPa. This method can be applied to any electroplating material such as gold and copper. The local nano-electrode will make a significant contribution to the development of more complicated and sophisticated nanofluidic electrophoresis systems and to local electric detection methods for various nanofluidic devices.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proposal and performance evaluation of a new parallel plate continuous cell separation device using dielectrophoresis 利用介电泳技术的新型平行板连续细胞分离装置的提案和性能评估。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-06-27 DOI: 10.1002/elps.202400027

Yoshinori Seki, Aoi Nagasaka, Tsukushi Gondo, Shigeru Tada

引用次数: 0

Development of capillary electrophoresis methods for the detection of microbial metabolites on potential future spaceflight missions 开发毛细管电泳方法，用于检测未来潜在航天飞行任务中的微生物代谢物。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-06-26 DOI: 10.1002/elps.202400073

Emily A. Kurfman, Maria F. Mora, Peter A. Willis, Susan M. Lunte

{"title":"Development of capillary electrophoresis methods for the detection of microbial metabolites on potential future spaceflight missions","authors":"Emily A. Kurfman, Maria F. Mora, Peter A. Willis, Susan M. Lunte","doi":"10.1002/elps.202400073","DOIUrl":"10.1002/elps.202400073","url":null,"abstract":"The search for chemical indicators of life is a fundamental component of potential future spaceflight missions to ocean worlds. Capillary electrophoresis (CE) is a useful separation method for the determination of the small organic molecules, such as amino acids and nucleobases, that could be used to help determine whether or not life is present in a sample collected during such missions. CE is under development for spaceflight applications using multiple detection systems, such as laser induced fluorescence (LIF) and mass spectrometry (MS). Here we report CE-based methods for separation and detection of major polar metabolites in cells, such as amino acids, nucleobases/sides, and oxidized and reduced glutathione using detectors that are less expensive alternatives to LIF and MS. Direct UV detection, indirect UV detection, and capacitvely coupled contactless conductivity detection (C4D) were tested with CE, and a combination of direct UV and C4D allowed the detection of the widest variety of metabolites. The optimized method was used to profile metabolites found in samples of Escherichia coli and Pseudoalteromonas haloplanktis and showed distinct differences between the species.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 19-20","pages":"1684-1691"},"PeriodicalIF":3.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141455963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of plasma affinity depletion methods: Impact on protein composition and phosphopeptide abundance in human plasma 血浆亲和力耗竭方法的比较分析：对人体血浆中蛋白质组成和磷酸肽丰度的影响

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-06-21 DOI: 10.1002/elps.202400030

Pallaprolu Nikhil, Dande Aishwarya, Sameer Dhingra, Krishna Pandey, V. Ravichandiran, Ramalingam Peraman

{"title":"Comparative analysis of plasma affinity depletion methods: Impact on protein composition and phosphopeptide abundance in human plasma","authors":"Pallaprolu Nikhil, Dande Aishwarya, Sameer Dhingra, Krishna Pandey, V. Ravichandiran, Ramalingam Peraman","doi":"10.1002/elps.202400030","DOIUrl":"10.1002/elps.202400030","url":null,"abstract":"Affinity-based protein depletion and TiO2 enrichment methods play a crucial role in detection of low-abundant proteins and phosphopeptides enrichment, respectively. Here, we assessed the effectiveness of HSA/IgG (HU2) and Human 7 (HU7) depletion methods and their impact on phosphopeptides coverage through comparative proteome analysis, utilizing in-solution digestion and nano-LC-Orbitrap mass spectrometry (MS). Our results demonstrated that both HU2 and HU7 affinity depletion significantly decreased high-abundant proteins by 1.5–7.8-fold (p < 0.001). A total of 1491 proteins were identified, with 48 proteins showing significant expression in the depleted groups. Notably, cadherin-13, neutrophil defensin 1, APM1, and desmoplakin variant protein were exclusively detected in the HU2/HU7-depleted groups. Furthermore, study on effect of depletion on phosphopeptides revealed an increase in tandem MS spectral counts with notable decrease (∼50%) in peptide spectrum matching in depleted groups, which was attributed to significant reduction in protein counts. Our post translation modification workflow for phosphoproteomics detected 42 phosphorylated peptides, corresponding to 12 phosphoproteins with unique peptide match ≥2 (high false discovery rates confidence). Among them, 10 phosphorylated proteins are highly expressed in depleted groups. Overall, these findings offer valuable insights in selection of protein depletion methods for comprehensive plasma proteomics analysis.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 19-20","pages":"1860-1873"},"PeriodicalIF":3.0,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141523775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of residual T7 RNA polymerase used in mRNA in vitro transcription by Simple Western 用 Simple Western 方法检测 mRNA 体外转录过程中使用的残余 T7 RNA 聚合酶。

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-06-20 DOI: 10.1002/elps.202400044

Yvonne Shieh, Andrew R. Swartz, Richard R. Rustandi

{"title":"Detection of residual T7 RNA polymerase used in mRNA in vitro transcription by Simple Western","authors":"Yvonne Shieh, Andrew R. Swartz, Richard R. Rustandi","doi":"10.1002/elps.202400044","DOIUrl":"10.1002/elps.202400044","url":null,"abstract":"Therapeutic messenger RNA (mRNA) has been demonstrated as a scalable and versatile vaccine platform for the rapid development and manufacture of new vaccine candidates. mRNA is synthesized enzymatically through in vitro transcription (IVT) using bacteriophage T7 RNA polymerase (T7 RNAP), a 99 kDa protein with high binding affinity for the promoter sequence and a low error rate. Post-IVT, mRNA is purified to remove impurities, but if T7 RNAP is insufficiently cleared, undesirable clinical side effects may result. Therefore, it is important to quantitate T7 RNAP concentrations in IVT and process intermediates to understand clearance during downstream purification. A high-throughput T7 RNAP assay was developed using Simple Western (SW), a capillary immunoassay technology, to quantitate concentrations as low as 5.3 ng/mL with good precision and accuracy. Compared to existing T7 RNAP immunoassays or total protein assays such as bicinchoninic acid assays or Bradford, the SW T7 RNAP assay is specific to T7 RNAP, requires <10 µL of sample volume, and consists of minimal sample handling and hands-on time. This work highlights the development and optimization of a highly sensitive and robust T7 RNAP quantitation assay using the SW platform.","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"45 19-20","pages":"1834-1839"},"PeriodicalIF":3.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202400044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141426594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Effects of nonlinear thermal radiation on magnetized A l 2 O 3 − B l o o d ${Al_2 O_3}-Blood$ nanofluid flow through an inclined microporous channel: An investigation of second law analysis” 对 "非线性热辐射对磁化 A l 2 O 3 - B l o o d ${Al_2 O_3}-Blood$ 纳米流体流经倾斜微孔通道的影响：第二定律分析研究"

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-06-20 DOI: 10.1002/elps.202400050

Amos W. Ogunsola, Mathew F. Oyedotun

引用次数: 0

Editorial Board: Electrophoresis 11–12'24 编辑委员会：电泳 11-12'24

IF 3 3区生物学

ELECTROPHORESIS Pub Date : 2024-06-20 DOI: 10.1002/elps.202470062

引用次数: 0