ELECTROPHORESIS最新文献

{"title":"A Continuum Modeling Approach to Nanoparticle Polarizability Characterization Using Fluorescence Intensity Profiles.","authors":"Mens Wout, Wood Jeffery A, Chengxun Liu, Lagae Liesbet, Willems Kherim","doi":"10.1002/elps.70087","DOIUrl":"10.1002/elps.70087","url":null,"abstract":"<p><p>The use of dielectrophoresis (DEP) for the manipulation of electrically polarizable particles has received a lot of interest in the past decades. Theories relying on the Clausius-Mossotti (CM) factor describe the DEP behavior of macroscale particles with great accuracy. However, under nanoscale conditions, these classical CM factor theories can break down. Therefore, experimental characterization of particle polarizabilities is of utmost importance. We present an integrated experimental-computational methodology that allows the quantification of effective particle polarizabilities from DEP experiments. The methodology is based on the comparison of experimental and simulated concentration profiles of fluorescent nanoparticles captured by a set of electrodes. We obtain effective particle polarizabilities for 52 and 105 nm particles that agree well with values predicted by CM theory. The considered particles hence serve as a calibration model for our approach, demonstrating that realistic particle polarizabilities can be obtained and paving the way for polarizability quantification for particles where existing theories are inadequate.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":"396-405"},"PeriodicalIF":2.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147485097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Biomimetic Protein Immobilization Method for Studying Drug-Protein Interactions Based on Affinity Capillary Electrochromatography.","authors":"Jiaping Cui, Yawen Tian, Xinru Fu, Xinhui Jiang, Kai Zhou, Zhining Xia, Yike Huang","doi":"10.1002/elps.70086","DOIUrl":"10.1002/elps.70086","url":null,"abstract":"<p><p>In complex systems, a universal and effective method for accurately determining the dissociation constant (K_d) of drug-protein interactions (DPI) has not been identified yet. In this study, a novel biomimetic affinity capillary electrochromatography (ACEC) platform was developed. Polydopamine (PDA) was employed not only as a coating material but also as an immobilization agent for human serum albumin (HSA), resulting in the fabrication of the PDA/PDA/HSA@capillary. A series of characterization experiments on the PDA/PDA/HSA@capillary showed that PDA was successfully coated on the inner wall of the capillary, and HSA was also successfully immobilized on the capillary column. The optimal concentration of PDA for preparing the PDA/PDA/HSA@capillary was determined to be 0.8 mg mL^-1, and the optimal concentration for immobilizing HSA was 0.25 mM, respectively. Moreover, the PDA/PDA/HSA@capillary showed good separation effects in the complex systems of different drugs. Furthermore, the electrophoretic performance of individual and mixed samples was compared across different capillary columns. Through interaction studies between proteins and compounds, the K_d value of rutin was 1.09 × 10³ mol L^-1 that of quercitrin was 2.78 × 10³ mol L^-1, and that of quercetin was 7.44 × 10⁴ mol L^- ¹, aligning with results from other established methods. The method was applied to the Sophora japonica extract, and the K_d was consistent with the rutin in the mixed system. Reproducibility studies demonstrated that high separation efficiency was maintained even after 50 consecutive runs. By integrating biomimetic material science with chromatographic innovation, this work overcomes critical bottlenecks in traditional affinity capillary electrophoresis (ACE), providing a universal tool for high-throughput drug screening and structure-guided therapeutic design.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":"373-386"},"PeriodicalIF":2.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Microfluidic Platform for Five-Order-of-Magnitude Concentration Control With Stable Droplet Size.","authors":"Sung-Jin Kim","doi":"10.1002/elps.70085","DOIUrl":"10.1002/elps.70085","url":null,"abstract":"<p><p>Generating monodisperse droplets with tunable concentrations is essential for a wide range of microfluidic applications. We present a platform that overcomes the trade-off between concentration range and droplet size stability using a passive, water-head-driven system with asymmetric fluidic resistances. This design effectively decouples concentration tuning from droplet generation within the tested regime by stabilizing the junction pressure to ensure a steady total aqueous flow rate. We demonstrate theoretically and experimentally that this results in monodisperse droplets of nearly constant size across the programmed concentration range. Concurrently, by adjusting a single passive pressure inlet, the system achieves continuous control of the concentration ratio over a five-order-of-magnitude dynamic range. This robust, simple method eliminates complex pumps, offering a powerful tool for high-throughput screening and combinatorial studies.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":"414-419"},"PeriodicalIF":2.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Signatures of Spontaneous Isolated Superior Mesenteric Artery Dissection: Indole Derivatives and Lysophospholipids as Novel Biomarkers","authors":"Lei Ji,&nbsp;Xun Wang,&nbsp;Yangyan He,&nbsp;Ziheng Wu,&nbsp;Donglin Li,&nbsp;Hongkun Zhang","doi":"10.1002/elps.70078","DOIUrl":"10.1002/elps.70078","url":null,"abstract":"<div>\u0000 \u0000 <p>Spontaneous isolated superior mesenteric artery dissection (SISMAD) is a rare but increasingly recognized vascular disorder that can lead to intestinal dysfunction with severe complications. Currently, no plasma biomarkers, particularly metabolomics-based markers, have been identified for SISMAD. To identify novel plasma biomarkers and potential therapeutic targets for SISMAD and its associated intestinal dysfunction, we analyzed plasma samples from 30 patients with SISMAD and 30 healthy controls. Blood was collected within 72 h of diagnosis. Ultra-high-performance liquid chromatography coupled with orbitrap mass spectrometry was used to profile metabolites, followed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) to analyze the data. A total of 123 and 128 metabolites were significantly changed in the positive and negative ion modes, respectively. Notably, tryptophan-related metabolites, including methyl 2-(1<i>H</i>-indol-3-yl) acetate and indole-3-pyruvic acid (IPA), were significantly reduced in the SISMAD group (<i>p</i> &lt; 0.001), with a strong correlation between the two (Corr. = 0.989, <i>p</i> &lt; 0.001). Pathway analysis revealed that glycerophospholipid metabolism was most enriched, primarily driven by lysophospholipids (LPLs), particularly lysophosphatidylethanolamine (LysoPE), which showed the highest fold change and diagnostic accuracy (area under the curve [AUC] = 0.986). This study identifies significant metabolic alterations in SISMAD, including reductions in indole derivatives (methyl 2-(1<i>H</i>-indol-3-yl) acetate and IPA and several LPLs, such as LysoPE, lysophosphatidylcholine (LysoPC), and lysophosphatidylglycerol (LysoPG). These findings highlight potential diagnostic biomarkers and therapeutic targets for SISMAD and its associated intestinal dysfunction, providing a promising avenue for clinical management and intervention.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 4","pages":"328-338"},"PeriodicalIF":2.5,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146164718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Board: Electrophoresis 4'26","authors":"","doi":"10.1002/elps.70095","DOIUrl":"10.1002/elps.70095","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 4","pages":"273-275"},"PeriodicalIF":2.5,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Genetic Profiling for Cattle Parentage Testing via Next-Generation Sequencing","authors":"Jiaojiao Geng,&nbsp;Jiajun Liu,&nbsp;Zhentang Liu,&nbsp;Zhiyong Liu,&nbsp;Chaobo Bao,&nbsp;Xiaohui Yan,&nbsp;Xiaoyue Wang,&nbsp;Zhanze Wu,&nbsp;Hongyu Sun,&nbsp;Riga Wu","doi":"10.1002/elps.70082","DOIUrl":"10.1002/elps.70082","url":null,"abstract":"<div>\u0000 \u0000 <p>Cattle are frequently involved in civil disputes and offenses. Currently, the paternity testing of cattle is mainly done by analysis of dinucleotide STR (di-STR) or SNPs. However, the di-STR marker usually produces many stutter artifacts that affect the accurate genotyping, and the polymorphic level of SNPs is not high enough. To overcome these limitations, we developed and validated a novel integrated next-generation sequencing (NGS) panel for cattle. It comprises 33 reported di-STRs and 30 newly identified tetranucleotide STRs (tetra-STRs), the mitochondrial displacement loop (D-loop) region, as well as cytochrome b (<i>CYTB</i>) and recombination-activating gene 1 (<i>RAG1</i>). The results showed a high genotyping success rate (99.6% on average) and balanced amplification efficiency across most STR loci. In addition, the panel's average genotyping consistency rate reached 96.4% between blood and hair samples. The total discrimination power and cumulative probability of exclusion for duos of this panel were 1–1.8 × 10⁻¹³ and 1–1.7 × 10⁻⁸, respectively. Application in paternity testing caseworks demonstrates its applicability and reliability for resolving paternity disputes. Overall, this study provided a cattle profiling NGS panel with a substantial advancement over current capillary electrophoresis (CE)-based systems, offering superior accuracy, multiplex capability, and efficiency for forensic genetic testing in cattle.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 4","pages":"339-352"},"PeriodicalIF":2.5,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147316759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capillary Zone Electrophoresis for the Analysis of Phenolic Preservatives in Plasma and Insulin Formulations Using Salting-Out Assisted Liquid–Liquid Extraction","authors":"Meriem Dadouch,&nbsp;Yoann Ladner,&nbsp;Catherine Perrin","doi":"10.1002/elps.70083","DOIUrl":"10.1002/elps.70083","url":null,"abstract":"<p>Phenolic preservatives are critical excipients in biopharmaceutical formulations such as insulin and its analogs. The determination of these analytes in insulin formulations and human plasma is crucial for various applications. This study reports the first use of a method combining salting-out assisted liquid–liquid extraction (SALLE) with capillary zone electrophoresis (CZE). The CZE-based separation of phenolic preservatives remains particularly challenging, as the alkaline conditions required for adequate resolution lead to elevated electric current and accelerated capillary deterioration. Analytical parameters, including the nature of the background electrolyte (BGE) and ionic strength, were optimized to ensure reliable separation of phenol, <i>m</i>-cresol, and the internal standard. SALLE was performed using acetonitrile (ACN) as the extraction solvent and ammonium acetate as the salting-out agent. CZE separation was completed in less than 6 min using a carbonate buffer at pH 10.3. The SALLE procedure yielded good recoveries (96%–102%) and repeatability (relative standard deviation [RSD] for concentration determination &lt; 5.5%). The SALLE–CZE–UV methodology showed excellent linearity (<i>r²</i> &gt; 0.994) and repeatability (RSD for corrected migration times: 0.4% for phenol and 0.5% for <i>m</i>-cresol; RSD for the ratio of corrected peak areas [analyte/internal standard]: 2.6% for phenol and 1.8% for <i>m</i>-cresol). Moreover, appropriate limits of detection (LOD) and quantification (LOQ) were obtained for insulin formulations in line with regulatory requirements. In addition, suitable LOD and LOQ (0.014; 0.047 g.L⁻¹ for phenol and 0.007; 0.022 g.L⁻¹ for <i>m</i>-cresol) were obtained for spiked human plasma.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 4","pages":"308-319"},"PeriodicalIF":2.5,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147431632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of Peak Integration in Capillary Electrophoresis: Reference Data Set No. 1","authors":"Marlon Krompholz,&nbsp;Timothy Blanc,&nbsp;Huixin Lu,&nbsp;Patricia Christensen,&nbsp;Frédéric Ginot,&nbsp;Gábor Járvás,&nbsp;Trang D. Nguyen,&nbsp;Ashley Prout,&nbsp;Timothy Riehlman,&nbsp;Brian Wei,&nbsp;Andrei Hutanu,&nbsp;Steffen Kiessig,&nbsp;Knut Baumann,&nbsp;Cari E. Sänger-van de Griend,&nbsp;Hermann Wätzig","doi":"10.1002/elps.70077","DOIUrl":"10.1002/elps.70077","url":null,"abstract":"<p>Capillary electrophoresis (CE) often provides superior separation of macromolecules such as monoclonal antibodies (mAbs), a major biopharmaceutical class, compared with liquid chromatography. However, electropherograms frequently exhibit complex baselines and peak shapes that are not reliably handled by integration algorithms designed for chromatographic data, and manual integration is often required. Many concepts have been proposed to improve peak integration, ranging from incremental algorithmic refinements and signal-to-noise (S/N)-based approaches to artificial intelligence (AI)-driven strategies, but objective performance comparisons are not possible without shared reference data sets and agreed peak limits. To address this gap, we initiated a multinational collaboration involving industrial and academic laboratories to create a comprehensive reference data set for CE peak integration. A total of 227 challenging and practically relevant electropherograms were collected from diverse applications, converted to a standardized format, and independently integrated by multiple experts. Using dedicated software tools and a structured consensus process, mutually accepted reference integration limits were established for each data set. These reference electropherograms, together with the underlying integration rules, are now made available to the scientific community. Analysis of the reference data set identified general principles for reliable peak integration, including the importance of standardized zoom levels and consistent handling of small peaks near the noise level. The data set provides a common foundation for benchmarking commercial chromatography data systems (CDS) and for developing and validating new algorithmic and AI-based integration methods. We expect this work to speed up the development of practical, automated integration strategies for CE and that these core concepts can be applied to other separation techniques.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 4","pages":"276-294"},"PeriodicalIF":2.5,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.70077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147431584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 4","pages":"273-275"},"PeriodicalIF":2.5,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148081798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrilineal Genetic Analysis of Tibeto-Burman-Speaking Populations in Guizhou","authors":"Shuaiji Pan,&nbsp;Yuhang Feng,&nbsp;Li Chen,&nbsp;Lin Wang,&nbsp;Cuiyun Le,&nbsp;Hongling Zhang,&nbsp;Xiaoye Jin,&nbsp;Qiyan Wang,&nbsp;Yubo Liu,&nbsp;Meiqing Yang,&nbsp;Wen Wan,&nbsp;Jiang Huang,&nbsp;Zheng Ren","doi":"10.1002/elps.70080","DOIUrl":"10.1002/elps.70080","url":null,"abstract":"<div>\u0000 \u0000 <p>Studies on the genetic characteristics of Tibeto-Burman (TB)-speaking populations have primarily concentrated on high-altitude Tibetan populations, while studies involving TB-speaking populations in Guizhou remain scarce. This research involved sequencing the whole mitochondrial genome of 321 individuals from TB-speaking populations in Guizhou, such as Bai, Yi, and Tujia. Haplotype diversity (Hd), molecular diversity indices, neutrality tests, mismatch distributions, and Bayesian skyline plots were utilized to evaluate genetic characteristics and population fluctuations. To investigate the genetic architecture of TB populations in Guizhou, analyses of population genetics were conducted utilizing complete mitochondrial genome sequences obtained from 91 worldwide populations. The TB-speaking populations in Guizhou exhibit a significant presence of prevalent southern haplogroups (49.53%) and prevalent northern haplogroups (36.45%). Genetic analyses revealed closer genetic relationships between Guizhou TB populations and Han populations of southern China, as well as Guizhou Tai-Kadai-speaking populations. This study fills the gap in maternal genetic research at the whole mitochondrial genome level for Guizhou TB populations and provides valuable data for population genetic studies of ethnic minorities in Guizhou.</p>\u0000 </div>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":"47 4","pages":"353-368"},"PeriodicalIF":2.5,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146164678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}