Development and Validation of the 9-Dye Fluorescent Autosomal and Y Chromosome Multiplex STR Amplification System

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-03-06 DOI:10.1002/elps.8130

Yu Hao, Zhang Zhe, Cui Yuling, Li Chenjia, Mo Xiaoting, Shang Lei, Song Wen, Jia Lina, Wang Jing, Li Wanshui, Bai Xue

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引用次数: 0

Abstract

To develop a 9-dye fluorescent autosomal and Y chromosome STR multiplex amplification system and verify its performance. Using the 9-dye fluorescent labeling technology, construct a multiplex amplification system that uses the autosomal and Y chromosome core and preferred loci recommended by GB/T 41009-2021 national standard as candidate loci, then evaluate its accuracy, sensitivity, species specificity, and other system performance index. A 9-dye fluorescent multiplex amplification system containing 70 loci, including Amel, D5S818, D21S11, D7S820, CS1FPO, D2S1338, D19S433, D8S1132, D2S441, VWA, D8S1179, D16S539, D10S1435, PentaD, D3S3045, TPOX, TH01, D22S1045, D18S51, FGA, D19S253, D10S1248, D6S477, D6S1043, D13S317, D12S391, D15S659, PentaE, D1S1656, D3S1358, DYS460, DYS389I, DYS390, DYS533, DYS392, DYS518, DYS557, DYS570, DYS458, DYS437, DYS385, GATA-H4, DYS576, DYS643, DYS508, DYS627, DYS456, DYS391, DYF404S1, DYS438, DYS448, DYF387S1, DYS596, DYS549, DYS399S1, DYS393, DYS635, DYS439, DYS19, DYS444, DYS449, DYS481, DYS527a/b, and DYS447 was successfully constructed. The various loci in this system are well balanced, with a sensitivity of 0.0625 ng and a certain ability to detect mixture samples. This system can obtain rich locus information through a single test and meet the needs of routine testing cases and DNA database construction. It has a good practical significance in forensic medicine.

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9-染料荧光常染色体和Y染色体多重STR扩增系统的建立和验证。

建立9染料荧光常染色体和Y染色体STR多重扩增系统并验证其性能。采用9染料荧光标记技术，以GB/T 41009-2021国家标准推荐的常染色体和Y染色体核心及优选位点为候选位点，构建多重扩增系统，并对其准确性、灵敏度、物种特异性等系统性能指标进行评价。一个包含70个基因座的9染料荧光多路扩增系统，包括Amel， D5S818, D21S11, D7S820, CS1FPO, D2S1338, D19S433, D8S1132, D2S441， VWA, D8S1179, d16s439, D10S1435, PentaD, D3S3045， TPOX, TH01, D22S1045, D18S51， FGA, D19S253, D10S1248, D6S477, D6S1043, D13S317, D12S391, D15S659, PentaE, D1S1656, D3S1358, DYS460, DYS389I, DYS390, DYS533, DYS518, DYS557, dys558, DYS437, DYS385, GATA-H4, DYS576, DYS643, DYS508, DYS627, DYS456, DYS391, DYF404S1, DYS438, DYS448，成功构建了DYF387S1、DYS596、DYS549、DYS399S1、DYS393、DYS635、DYS439、DYS19、DYS444、DYS449、DYS481、DYS527a/b和DYS447。该系统各基因座平衡良好，灵敏度为0.0625 ng，对混合样品有一定的检测能力。该系统通过一次检测即可获得丰富的基因座信息，满足常规检测案例和DNA数据库建设的需要。在法医学中具有很好的实践意义。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.